马齿苋提取物治疗急性湿疹的药效研究及作用机理实验研究
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摘要
目的：观察马齿苋提取物对湿疹大鼠皮肤的治疗效果,探讨单品马齿苋的药效以及促进湿疹病变部位修复的作用机理。
方法：
1.SD大鼠70只,按体重随机分为7个组,每组10只,分别作为正常组、模型组、Caspase-14基因敲除组、阳性组、马齿苋提取物高、中、低剂量组。除正常组外,用电推剪及电剃须刀去除腹部毛发,面积约2cm×3cm,并涂5%的2,4-二硝基氯苯(DNCB)30μl于皮肤首次致敏；第5天,在右背部2cm×2cm剃毛区涂0.2%DNCB50μl进行二次抗原攻击,左背部对称的剃毛区不用药物作为对照部位。以后每隔3d激发1次,连续3次。于第9天观察大鼠右背部较左背部明显出现红斑,水肿,丘疹,糜烂或渗出,表明造模成功。实验第21天断颈处死,剪取造模皮肤,用分析天平称重测量湿疹大鼠皮肤肿胀度；QRT-PCR检测各实验组大鼠皮肤Caspase-14和Filaggrin基因表达；免疫组化检测TNF-α、IL-22、IL-4、IFN-γ积分光密度。
2.另取大鼠50只,随机分为5组：正常对照组,阳性对照组,马齿苋低剂量组、中剂量组、高剂量组。各组按规定的剂量外用给药。实验当日,用0号砂纸擦伤大鼠剃毛处,局部再涂各组相应药物1次,末次涂药后10min,开始于创面处滴0.01%磷酸组胺每只0.05mL,此后每隔3min依0.01%,0.02%,0.03%,0.04%…浓度递增,每一浓度滴加后观察3min内大鼠是否回头舔右后足背,按此时磷酸组胺总量计算各组大鼠的致痒阂。
结果：
1.与空白对照组相比,高剂量组小鼠皮肤肿胀度明显小于空白对照组(P<0.01),中剂量组、阳性药物组小鼠皮肤肿胀度也小于模型组(P<0.05)。
2.与正常组比较,模型组和Caspase-14基因敲除组大鼠皮肤组织细胞中Caspase-14和Filaggrin基因的2-ΔΔCT值极显著减小(P<0.01),与模型组和Caspase-14基因敲除组比较,马齿苋三个剂量组大鼠皮肤组织细胞中Caspase-14和Filaggrin基因的2-ΔΔCT值极显著增大(P<0.01)。
3.与正常组比较,模型组大鼠皮肤组织TNF-α免疫组织化学检测积分光密度(IOD)极显著增大(P<0.01)；模型组大鼠皮肤组织IL-22免疫组织化学检测积分光密度(IOD)极显著增大(P<0.01)；模型组大鼠皮肤组织IL-4免疫组织化学检测积分光密度(IOD)极显著增大(P<0.01)；模型组大鼠皮肤组织IFN-γ免疫组织化学检测积分光密度(IOD)显著减小(P<0.05)。
4.磷酸组胺对不同剂量组的致痒阈记录显示,马齿苋提取物中剂量组的致痒阈高于阳性对照组(P<0.05),高剂量组明显高于阳性对照组(P<0.05)。
结论：马齿苋提取物可以使血管收缩,抑制毛细血管通透性,抑制炎性肿胀,从而减轻湿疹局部的瘙痒、水肿症状。马齿苋提取物还可显著上调湿疹动物皮肤屏障系统中Caspase-14和Filaggrin基因的表达,提示马齿苋通过促进Caspase-14基因的表达而加速ProFilaggrin去磷酸化成为Filaggrin,进而加速Filaggrin水解成各种天然保湿因子,维护皮肤屏障功能。同时,马齿苋提取液通过降低TNF-a和IL-22在湿疹发病过程中的表达,提高IFN-γ的分泌抑制IL-4的表达,增强了机体的细胞免疫功能。
Objective To investigate the impact of the Purslane on the eczema skin, explore the effect and mechanism of single Purslane on the repairing lesion site of eczema
Methods
1.70SD rats were randomly divided into control group, model group, Caspase-14gene knockout group, positive group and high, medium, low dose of Purslane group. Except The normal group, the hair of rat's abdomen were shaved by electric clippers and electric shaver, the area is2cm X3cm, then,2,4-dinitrochlorobenzene was used to make rats acur first sensitization. After5days,2,4-dinitrochlorobenzene was used again on the right back of rat, the area is2cm X2cm, the left back as the control site. To use excitation on rat every3days,3times in a row. At the9th day, the right back has erythema, edema, erosion or exudative papules, it showed eczema model was made successful.21days later, all animals were sacrificed, eczema skins were taken out, analytical balance was used to measure swelling of eczema skin, QRT-PCR was used to detect the expression of Caspase-14and Filaggrin gene in each experimental group rat skin. Immunohistochemical detection was ued to measure TNF-α、IL-22、IL-4. IFN-γ
2.50SD rats were randomly divided into control group, positive group and high, medium, low dose of Purslane group. The recommended external dosage were given. At the experimental day,0standard Sandpaper was used to scrape the right leg of rat, then, all drug were applied again, after10minutes, the0.01%histamine phosphate was applied on the scraping area, more and more concentrated histamine phosphate were used every3minutes,0.01%,0.02%,0.03%,0.04%...The concentration is the itch threshold when the rat lick the right foot back.
Results:
1. Compared with the blank control group, swelling of high dose group was significantly less than the control group (P<0.01), The medium dose group, positive drug group is lower than model group (P<0.05).
2. Compared with the normal group, Caspase-14and Filaggrin cells' value in skin tissue of model group and knockout was significantly decreased (P<0.01). Compared with the medel group Caspase-14gene knockout group, Caspase-14and Filaggrin gene in the three dose of Purslane groups was significantly increased (P<0.01).
3. Compared with the normal group, IOD of TNF-α, IL-22, IL-4of model group was significantly increased (P<0.01), the IOD of IFN-γ was significantly decreased,(P<0.01); but the IOD of TNF-α, IL-22, IL-4of high and medium group was decreased (P<0.05), IFN-γ was decreased,(P<0.05);
4. The histamine phosphate in different dose groups of itching threshold records showed the medium group was higher than positive group(P<0.05), the high group was significantly higher than positive group(P<0.01),
Conclusion Purslane extract can constrict the blood vessels, inhibit capillary permeability, inhibit the inflammatory swelling, reduce the itching and swelling symptoms of eczema edema. Purslane extract can raise the expression of Caspase-14and Filaggrin of skin barrier system, it means Purslane extract can improve gene expressionthe of Caspase-14to accelerate dephosphorylation of ProFilaggrin to transformed into filaggrin, accelerating the hydrolysis of Filaggrin into a variety of natural moisturizing factor to enhance skin protective function. At the same time, Purslane extract decrease the expression of TNF-a and IL-22in the eczema, and increase the secretion of IFN-γ to inhibit IL-4, then, the cellular immune function was enhance.
方法：
1.SD大鼠70只,按体重随机分为7个组,每组10只,分别作为正常组、模型组、Caspase-14基因敲除组、阳性组、马齿苋提取物高、中、低剂量组。除正常组外,用电推剪及电剃须刀去除腹部毛发,面积约2cm×3cm,并涂5%的2,4-二硝基氯苯(DNCB)30μl于皮肤首次致敏；第5天,在右背部2cm×2cm剃毛区涂0.2%DNCB50μl进行二次抗原攻击,左背部对称的剃毛区不用药物作为对照部位。以后每隔3d激发1次,连续3次。于第9天观察大鼠右背部较左背部明显出现红斑,水肿,丘疹,糜烂或渗出,表明造模成功。实验第21天断颈处死,剪取造模皮肤,用分析天平称重测量湿疹大鼠皮肤肿胀度；QRT-PCR检测各实验组大鼠皮肤Caspase-14和Filaggrin基因表达；免疫组化检测TNF-α、IL-22、IL-4、IFN-γ积分光密度。
2.另取大鼠50只,随机分为5组：正常对照组,阳性对照组,马齿苋低剂量组、中剂量组、高剂量组。各组按规定的剂量外用给药。实验当日,用0号砂纸擦伤大鼠剃毛处,局部再涂各组相应药物1次,末次涂药后10min,开始于创面处滴0.01%磷酸组胺每只0.05mL,此后每隔3min依0.01%,0.02%,0.03%,0.04%…浓度递增,每一浓度滴加后观察3min内大鼠是否回头舔右后足背,按此时磷酸组胺总量计算各组大鼠的致痒阂。
结果：
1.与空白对照组相比,高剂量组小鼠皮肤肿胀度明显小于空白对照组(P<0.01),中剂量组、阳性药物组小鼠皮肤肿胀度也小于模型组(P<0.05)。
2.与正常组比较,模型组和Caspase-14基因敲除组大鼠皮肤组织细胞中Caspase-14和Filaggrin基因的2-ΔΔCT值极显著减小(P<0.01),与模型组和Caspase-14基因敲除组比较,马齿苋三个剂量组大鼠皮肤组织细胞中Caspase-14和Filaggrin基因的2-ΔΔCT值极显著增大(P<0.01)。
3.与正常组比较,模型组大鼠皮肤组织TNF-α免疫组织化学检测积分光密度(IOD)极显著增大(P<0.01)；模型组大鼠皮肤组织IL-22免疫组织化学检测积分光密度(IOD)极显著增大(P<0.01)；模型组大鼠皮肤组织IL-4免疫组织化学检测积分光密度(IOD)极显著增大(P<0.01)；模型组大鼠皮肤组织IFN-γ免疫组织化学检测积分光密度(IOD)显著减小(P<0.05)。
4.磷酸组胺对不同剂量组的致痒阈记录显示,马齿苋提取物中剂量组的致痒阈高于阳性对照组(P<0.05),高剂量组明显高于阳性对照组(P<0.05)。
结论：马齿苋提取物可以使血管收缩,抑制毛细血管通透性,抑制炎性肿胀,从而减轻湿疹局部的瘙痒、水肿症状。马齿苋提取物还可显著上调湿疹动物皮肤屏障系统中Caspase-14和Filaggrin基因的表达,提示马齿苋通过促进Caspase-14基因的表达而加速ProFilaggrin去磷酸化成为Filaggrin,进而加速Filaggrin水解成各种天然保湿因子,维护皮肤屏障功能。同时,马齿苋提取液通过降低TNF-a和IL-22在湿疹发病过程中的表达,提高IFN-γ的分泌抑制IL-4的表达,增强了机体的细胞免疫功能。
Objective To investigate the impact of the Purslane on the eczema skin, explore the effect and mechanism of single Purslane on the repairing lesion site of eczema
Methods
1.70SD rats were randomly divided into control group, model group, Caspase-14gene knockout group, positive group and high, medium, low dose of Purslane group. Except The normal group, the hair of rat's abdomen were shaved by electric clippers and electric shaver, the area is2cm X3cm, then,2,4-dinitrochlorobenzene was used to make rats acur first sensitization. After5days,2,4-dinitrochlorobenzene was used again on the right back of rat, the area is2cm X2cm, the left back as the control site. To use excitation on rat every3days,3times in a row. At the9th day, the right back has erythema, edema, erosion or exudative papules, it showed eczema model was made successful.21days later, all animals were sacrificed, eczema skins were taken out, analytical balance was used to measure swelling of eczema skin, QRT-PCR was used to detect the expression of Caspase-14and Filaggrin gene in each experimental group rat skin. Immunohistochemical detection was ued to measure TNF-α、IL-22、IL-4. IFN-γ
2.50SD rats were randomly divided into control group, positive group and high, medium, low dose of Purslane group. The recommended external dosage were given. At the experimental day,0standard Sandpaper was used to scrape the right leg of rat, then, all drug were applied again, after10minutes, the0.01%histamine phosphate was applied on the scraping area, more and more concentrated histamine phosphate were used every3minutes,0.01%,0.02%,0.03%,0.04%...The concentration is the itch threshold when the rat lick the right foot back.
Results:
1. Compared with the blank control group, swelling of high dose group was significantly less than the control group (P<0.01), The medium dose group, positive drug group is lower than model group (P<0.05).
2. Compared with the normal group, Caspase-14and Filaggrin cells' value in skin tissue of model group and knockout was significantly decreased (P<0.01). Compared with the medel group Caspase-14gene knockout group, Caspase-14and Filaggrin gene in the three dose of Purslane groups was significantly increased (P<0.01).
3. Compared with the normal group, IOD of TNF-α, IL-22, IL-4of model group was significantly increased (P<0.01), the IOD of IFN-γ was significantly decreased,(P<0.01); but the IOD of TNF-α, IL-22, IL-4of high and medium group was decreased (P<0.05), IFN-γ was decreased,(P<0.05);
4. The histamine phosphate in different dose groups of itching threshold records showed the medium group was higher than positive group(P<0.05), the high group was significantly higher than positive group(P<0.01),
Conclusion Purslane extract can constrict the blood vessels, inhibit capillary permeability, inhibit the inflammatory swelling, reduce the itching and swelling symptoms of eczema edema. Purslane extract can raise the expression of Caspase-14and Filaggrin of skin barrier system, it means Purslane extract can improve gene expressionthe of Caspase-14to accelerate dephosphorylation of ProFilaggrin to transformed into filaggrin, accelerating the hydrolysis of Filaggrin into a variety of natural moisturizing factor to enhance skin protective function. At the same time, Purslane extract decrease the expression of TNF-a and IL-22in the eczema, and increase the secretion of IFN-γ to inhibit IL-4, then, the cellular immune function was enhance.
引文
[1]徐拯,周江睿,孙学军.氢生理盐水对小鼠足肿胀的抗炎效应.现代生物医学进展,2010,10(23)：4421-4423.
[2]Ui H, Andoh T, Lee JB, etal. Potent pruritogenic action of tryptase mediated by PAR-2 receptor and its involvement on anti-pruritic effect of nafamostat mesilate in mice[J]. Eur J Pharmacol,2006,530 (1-2):172-178.
[3]邓小元.皮肤源性瘙痒发生机制的研究进展.中国医药科学,2012,2(3)：42-43其中组胺是公认的、经典的致痒介质.
[4]Segre JA. Epidermal barrier formation and recovery in skin disorders[J]. ClinInvest,2006,116(5):1150-1158.
[5]Katoh M, Hamajima F,Ogasawara T, etal. Assessment of human epidermal model LabCyte EPI-MODEL for in vitro,skin irritation testing according to European Centre for the Validation of Alternative Methods(ECVAM)-validate protocol[J]. Toxicol Sci,2009,34(3):327-334.
[6]张芳芳,车雅敏,傅志宜Filaggrin和Caspase-14在皮肤屏障中的作用.皮肤性病诊疗学杂志,2012,19(2)：114-117.
[7]Ballardini N, Kull I, Soderhall C, Eczema severity in preadolescent children and its relation to sex, filaggrin mutations, asthma, rhinitis, aggravating factors and topical treatment:a report from the BAMSE birth cohort. Br J Dermatol.2013 Mar;168(3):588-94.
[8]Sandilands A, Sutherland C, Irvine AD, etal. Filaggrin in the frontline: role in skin barrier function and disease[J]. Cell Sci,2009, 22(9):1285-1294.
[9]Eckhart L, Ban J,Fischer H, etal. Caspase-14:analysis of gene structure and mRNA expression during keratinocyte differentiation[J]. Biochem Biophys Res Commun,2000,277(3):655-659.
[10]Alibardi L, Tschachler E, Eckhart L. Distribution of Caspase-14 in epidermis and hair follicles is evolutionarilyconserved among mammals[J].Anat Rec A Discov Mol Cell Evol Biol,2005,286(2):962-973.
[11]李军,吴伽.一氧化氮对小鼠皮肤Caspase-14表达及屏障功能的影响.吉林医学,201莹31(10)：1301-1302.
[12]Denecker G, Hoste E, Gilbert B, et al. Caspase-14 protects against epidermal UVB photo damage and water loss[J]. Nat Cell Biol,2007,9(4):666.
[13]Landeck L, Visser M, Kezic S, Genotype-phenotype associations in filaggrin loss-of-function mutation carriers. Contact Dermatitis.2013 Mar; 68 (3):149-55.
[14]Thyssen JP, Linneberg A, Ross-Hansen K. Filaggrin mutations are strongly associated with contact sensitization in individuals with dermatitis.Contact Dermatitis.2013, Jan;24.
[15]Segre JA. Epidermal barrier formation and recovery in skin dis-orders[J].J Clin Invest,2006,116:1150-1158.
[16]董海胜,陈斌.茶树籽油与马齿苋籽油中脂肪酸及维生素E组成分析.营养学报,2012,34(1)：94-95.
[17]起珏,庞勤,涂颖.含马齿苋、青刺果提取物护肤品对激素依赖性皮炎辅助治疗作用的临床观察.2011全国中西医结合皮肤性病学术会议论文汇编.
[18]Marly JP. NMF and cosmetology of cutaneous hydration [J]. Ann Derm atol Venereol,2002,129:131-136.
[19]Nakagawa N, Sakai S, Matsumoto M, etal. Relationship between NMF (lactate and potassium)content and the physical properties of the stratum corneum in healthy subjects [J].J Invest Dermatol,2004,122:755-763.
[20]杨怀珠.雷公藤多贰对湿疹患者T淋巴细胞亚群的影响.中国中西医结合杂志,2007,27(7)：592.
[21]宋瑜,李咏梅,马绍尧,等.除湿止痒合剂对变应性接触性皮炎小鼠模型.Thl／Th2相关细胞因子水平的影响仁[T].成都中医药大学学报,2008,31(1)：32-3.
[22]Drago L, Toscano M, De Vecchi E, Changing of fecal flora and clinical effect of L. salivarius LS01 in adults with atopic dermatitis.J Clin Gastroenterol.2012 Oct,46 Suppl:S56-63.
[23]Sabin BR, Peters N, Peters AT. Chapter 20:Atopic dermatitis. Allergy Asthma Proc.2012 May-Jun;33.
[24]邢艳玲,张玉环,许建.工P--10对变应性接触性皮炎小鼠Thl／ThZ型细胞因子表达的影响[J].天津医科大学学报,2008,14(4)：492-494.
[25]王远红,王相明,等.中西结合治疗湿疹近况[J].黑龙江中医药,2009,38(3)：54-56.
[26]王远红,焦彦民,孙惠敏.除湿饮治疗湿疹(湿热型)的临床观察及对血清TNF-a水平的影响.黑龙江中医药,2011,(5)：28.
[27]吴华勋,魏伟.Th22细胞在炎症免疫性疾病中作用的研究进展.免疫学杂志,2012,28(9)：816-817.
[28]Fujita H. The role of IL-22 in the pathogenesis of skin diseases. Nihon Rinsho Meneki Gakkai Kaishi.2012,35(3):168-75.
[29]吕晶,徐军发.Th22细胞：一种新型的辅助型T细胞.中国热带医学,2011.11(1).
[30]Eyerich S, Eyerich K, Pennino D, etal. Th22 cellsrepresent a distinct human T cell subset involved inepidermalimmunity and remodeling[J]. J Clin Invest,2009,119(12):3573-3585.
[31]Stefanie E, Kilian E, Davide P, etal. Th22 cells represent a distinct human T cell subset involved in epidermal immunity and remodel ing [J]. J. Clin.Invest,2009,119:3573-3585.
[32]Kerstan A, Brocker EB, Trautmann A. Decisive role of tumor necrosis factor-a for spongiosis formation in acute eczematous dermatitis. Arch Dermatol Res.2011 Nov;303(9):651-8.
[33]Ngatu NR, Okajima MK, Yokogawa M, Anti-allergic effects of Vernonia amygdalina leaf extracts in hapten-induced atopic dermatitis-like disease in mice. Allergol Int.2012 Dec;61(4):597-607.
[34]van Hamburg JP, Corneth OB, Paulissen SM. IL-17/Th17 mediated synovial inflammation is IL-22 independent. Ann Rheum Dis.2013, Jan 17.
[35]NogralesKE, ZabaLC, ShemerA, etal. IL-22-produeing "T22" TeellsaeeountforuPregulatedIL-22inatopiedermatitisdespiteredueed IL-17 rodueing TH17 Teells. JA11. rgyClinInununol.2009,123(6):1244-52.
[36]Jabbari A, Johnson-Huang LM, Krueger JG. Role of the immune system and immunological circuits in psoriasis.G Ital Dermatol Venereol.2011 Feb; 146(1):17-30.
[37]Ring J, Mohrenschlager M, Weidinger S. Molecular genetics of atopic eczema. Chem Immunol Allergy.2012,96:24-9.
[38]Rebane A, Zimmermann M, Aab A. Mechanisms of IFN-γ-induced apoptosis of human skin keratinocytes in patients with atopic dermatitis. J Allergy Clin Immunol.2012 May;129(5):1297-306.
[39]Leung DY,Gao PS, Grigoryev DN,Human atopic dermatitis complicated by eczema herpeticum is associated with abnormalities in IFN-γ response. J Allergy Clin Immunol.2011 Apr;127(4):965-73.
[40]Werfel T.The role of leukocytes, keratinocytes, and allergen-speciIgE in the development of atopic dermatitis [J]. J Invest Dermatol,2009,129(8):1878-91.
[41]Heninger E, Falus A, Darvas Z, etal. Both interferon (IFN) alpha and IFN gamma inhibit histidine decarboxylase expression in the HT168 human melanoma cell line. Inflammation Res,2000,49(8):393-397.
[42]张玉环,陈保疆,焦振山,等.特应性皮炎患者单个核细胞Thl/Th2和IFN-Y及IL-4 mRNA表达[J].天津医药,2006,34(12)：833-834.
[43]Yang XY, Wang HY, Zhao XY,Th22, but not Th17 Might be a Good Index to Predict the Tissue Involvement of Systemic Lupus Erythematosus. J Clin Immunol.2013 Feb,23.
[44]KarinM. Nuclearfaetor-kaPPaBincaneerdeveloPmentandProgression[J]. N atural,2006,441(7092):431-436.
[45]Jennifer L, Katia B, Rene de Waal M.Development and function of TH17 cells in health and disease[J]. J Allergy ClinImmunol,2009,123:1004-1011.
[46]陆丽明,程楷涛.湿疹发生机理的研究进展.亚太传统医 药,2007,3(9)：76-78.
[47]Wang H, Mu W, Ja Q, Cytokine Profile of a Self-Healing Fonsecaea pedrosoi Infection in Murine Model.Cell Biochem Biophys.2013 Mar,12.
[48]Ali R, Kumar S, Naqvi RA, B and T cell epitope mapping and study the humoral and cell mediated immune response to B-T constructs of YscF antigen of Yersinia pestis. Comp Immunol Microbiol Infect Dis.2013
[49]李丽,甄莉.黄芪对湿疹大鼠皮损中白细胞介素-4和干扰素-Y影响的实验研究.中国药物与临床,2010,10(1).
[50]林星游,王炎,翁雪云.湿疹的中医辨治探析.中国当代医药,2009,16(11).
[51]罗杰,漆永平,喻中明.紫草荆防汤湿敷治疗创伤后湿疹疗效观察.现代中西医结合杂志,2012,21(7)：721-722.
[52]Epidemiology of human atopic dermatitis--seven areas of notable progress and seven areas of notable ignorance. Williams HC. Vet Dermatol. 2013 Feb,24(1):3-9.
[53]张振奎.婴儿湿疹情况调查分析[J].医药论坛杂志,2006,27(2)：42.
[54]Oral H1 antihistamines as monotherapy for eczema. Apfelbacher CJ, van Zuuren EJ, Fedorowicz Z, etc.2013 Feb,28(2):CD007770.
[55]Maternal fish consumption during pregnancy and risks of wheezing and eczema in childhood:The Generation R Study. Leermakers ET, Sonnenschein-van der Voort AM, Heppe DH, etc. Eur J Clin Nutr.2013 Feb,27.
[56]Invited commentary:childhood acute lymphoblastic leukemia and allergies:biology or biasL inabery AM, Spector LG. Am J Epidemiol.2012 Dec 1,176(11):979-83.
[57]Thyssen JP, Johansen JD, Zachariae C, Xerosis is Associated with Atopic Dermatitis, Hand Eczema and Contact Sensitization Independent of Filaggrin Gene Mutations.Acta Derm Venereol.2013, Feb,18.
[58]匡玉珍,刘志军,段明.衡阳地区荨麻疹和湿疹患者过敏原检测分析.南华大学学报(医学版),2叭0,38(2)：236-238.
[59]Kang K, Stevens SR. Pathophysiology of atopic dermatitis[J]. ClinDermatol,2003,21:116-121.
[60]盛楠,宗文凯,余美文,等.咪唑斯汀治疗湿疹和特应性皮炎临床疗效及免疫机制的初步研究[J].临床皮肤科杂志,2009,38(7)：440-442.
[61]李邻峰.微生物变态反应与微生物性湿疹[J].岭南皮肤性病科杂志,2009,16(4)：211-212.
[62]DahlMV. Clinical demato logy[M].3rded. Mosby Si Louis,1996,20.
[63]Wehner J, Neuber K. Staphy Lococcus aureus enterotoxins inducehistam in e and leukotrien e release in patients with atopic eczema[J].B r J Dem ato,2001,145(2):302-305.
[64]赵凤臣,吴洪军,潘高荣.马齿苋栽培技术.中国林副特产,2002,63(4)：11.
[65]李秀花,高志花,靳玲品.马齿苋的营养及应用.畜牧与兽医,2006,38(8)：34-35.
[66]黄海辉.感染性腹泻的经验治疗[J].中国临床医生,2002,30(7)：5-6
[67]侯俊然,范迎.马齿苋体外抗感染性腹泻菌群的作用研究.中外医疗,2010,28：29.
[68]张秀娟,季宇彬,曲中原,等.马齿苋体外抗茵作用的实验研究.中国微生态学杂志,2002,14(5)：277-279.
[69]李晓,冯雷,胡尊丽.马齿苋多糖的抗氧化活性研究.中国生化药物杂志,2010,31(4)：244-245.
[70]Lee AS, Kim JS, Lee YJ, Kang DG,Anti-TNF-a Activity of Portulaca oleracea in Vascular Endothelial Cells. Int J Mol Sci.2012; 13(5):5628-44.
[71]向兰,邢东明,王伟.马齿苋的化学成分研究进展.亚太传统医药,2006,7：64-68.
[72]崔曼,尹苗,安利国.马齿苋多糖的抗肿瘤活性.山东师大学报(自然科学版),2002,17(1)：73-75.
[73]杨桂芹,王长泉.马齿苋甜菜红素抗肿瘤实验研究.时珍国医国药,2010,21(2)：388-389.
[74]史仁玖,苗苗,李艳玲.不同生长期马齿苋ω-3脂肪酸及矿物质元素含量分析.营养学报,2010,32(4)：404-406.
[75]Wanyin W, Liwei D, Lin J, etc. Ethanol extract of Portulaca oleracea L. protects against hypoxia-induced neuro damage through modulating endogenous erythropoietin expression. J Nutr Biochem.2012,23(4):385-91.
[76]余南才,黄浩,肖宏.马齿苋水提液抗D-半乳糖诱导的脑神经毒性损伤作用机制.科技成果,2009.
[77]康洁.马齿苋多糖对胎鼠大脑皮层细胞保护作用的研究.现代预防医学,2010,37(4)：747-748.
[78]高红梅,陈新,王月珍.马齿苋降血糖有效部位研究.长春中医药大学学报,2012,28(3)：536.
[79]Shobeiri SF, Sharei S, Heidari A. Portulaca oleracea L. in the treatment of patients with abnormal uterine bleeding:a pilot clinical trial.Phytother Res.2009 Oct,23(10):1411-4.
[80]金英子,张红英,付柳静,等.马齿苋提取物的抗炎消肿及镇痛作用研究[J].延边大学医学学报,2008,31(4)：259-260.
[81]王国玉,王浩字,佟继铭.马齿苋的化学成分与药理作用研究现状.承德医学院学报,2012,29(1)：82-84.
[82]朱丽,徐为公,赵广荣.马齿苋的研究现状与综合开发利用.北林果研究,2006,21(2).
[83]朱晓宦,吴向阳,仰榴青.马齿苋的化学成分和药理活性研究进展.常熟理工学院学报(自然科学版),2007,21(4).
[84]Y. P. Shi, Yan-Ping Sh, i Juan Chen, etc. Determination ofnoradrenaline and dopamine in Chinese herbalextracts from Por-tulaca oleracea L by high-performanceliquid chromatography[J]. ChromatographyA,2003,1003: 127-132.
[85]李湘力,黄鸣清,李万瑶.马齿苋治疗蜂毒过敏反应的实验研究[J].海峡药学,2007,19(6)：39-41.
[86]陆霄鹤,等.马齿苋及鱼腥草水提取液体外抑菌实验.抗感染药学,2010,7(1).
[87]杨柳,等.马齿苋提取物体外清除自由基活性的研究.药学服务与研究, 2011,11(3).
[88]董立巍,王万银,岳义田.马齿苋总黄酮抗小鼠缺氧作用及其机制研究.中西医结合学报,2005,3(6).
[89]佘鸿燕,王见宾,刘福来.复方马齿苋溶液治疗婴儿急性湿疹的临床对比研究.时珍国医国药,2007,18(12).
[90]马玲,李月红,刘红霞.除湿解毒汤治疗湿热型急性湿疹的疗效及对血清总IgE水平的影响.新疆医科大学学报,2010,33(12).
[91]李元红.复方黄柏液皮肤科新用[J].中国中医急诊,2006,15(6)：627-672.
[92]魏红英,刘景桢.复方黄柏液治疗婴儿湿疹疗效观察.皮肤病与性病,2011,33(4).
[93]曾晓会,杜铁良,陈玉兴.小儿湿疹软膏对动物接触性湿疹模型的影响.江西中医学院学报,2012,2,24(1)：50-51.
[94]Man M Q,Hupe M, Sun R, Topical apigenin alleviates cutaneous inflammation in murine models. Evid Based Complement Alternat Med. 2012,2012:912028.
[95]于均峰,朱启星.三氯乙烯引起BALB/c裸鼠刺激性接触性皮炎作用机制的研究.中国职业医学,2009,2,36(1).
[96]Stewart I,Seawright AA, Schluter PJ, Primary irritant and delayed-contact hypersensitivity reactions to the freshwater cyanobacterium Cylindrospermopsis raciborskii and its associated toxin cylindrospermopsin. BMC Dermatol.2006 Mar 31(6):5.
[97]宗桂珍,王冰,李德凤.系统性光变态反应检测动物模型的建立.中国比较医学杂志,2009,19(1)：22-24.
[98]Greenspoon J, Ahluwalia R, Juma N, Allergic and photoallergic contact dermatitis:a 10-year experience. Dermatitis.2013 Jan-Feb;24(1):29-32.
[99]Conti R, Bassi A, Difonzo EM, A case of photoallergic contact dermatitis caused by unusual exposure to ketoprofen. Dermatitis.2012 Nov-Dec;23(6):295-6.
[100]李秀芳,金若敏,符胜光.2种品系大鼠被动皮肤过敏反应的差异性比较.中 国实验方剂学杂志,2010,16(10)：102-103.
[101]Cho IH, Lee MJ, Kim JH, Fritillaria ussuriensis extract inhibits the production of inflammatory cytokine and MAPKs in mast cells. Biosci Biotechnol Biochem.2011;75(8):1440-5.
[102]Kim SR, Choi HS, Seo HS. Topical application of herbal mixture extract inhibits ovalbumin- or 2,4-dinitrochlorobenzene-induced atopic dermatitis. Evid Based Complement Alternat Med.2012:545497.
[103]马松阳,薛东运.应用1%吡美莫司乳膏治疗遗传性过敏性皮炎临床分析.中国社区医师·医学专业,2010,22(12)：247.
[104]张玉锁.抗过敏汤治疗急性湿疹的实验研究.中华中医药学刊,2008,26(12).
[105]林清,张建军,李伟.五倍子与黄柏外用治疗急性湿疹的配伍合理性研究.中华中医药杂志(原中国医药学报),2011,26(9).
[106]芦源.小鼠背部慢性皮炎一湿疹模型的初步研究.辽宁中医药大学学报,2009,11(10).
[107]王国江,顾军.小鼠背部变应性接触性皮炎模型的建立[J].中国职业医学,2004,31(1)：17-19.
[1]徐晶钰,张璇,魏品康.中医药治疗湿疹合并抑郁症思路[J].中国中医药信息杂志,2011,8(10)：95.
[2]刘纯莉,李红军,尹东辉.活血化瘀法治疗慢性湿疹综述[J].北京中医药大学学报：中医临床版,2011,18(1)：44-45.
[3]胡赞,朱国强.湿疮病辨治及用药心得.光明中医,2012,2,27(2):CJGMCM February 2012. Vol27.2.
[4]李咏梅,宋瑜,马绍尧.脏腑辨证治疗湿疹265例临床观察[J].浙江中西医结合杂志,2004,14(9)：547-549.
[5]赵丽平.中医辨证论治湿疹86例[J].中国中医药信息杂志,2000,7(7)：66-67.
[6]宋瑜,马绍尧,李咏梅.健脾除湿方治疗湿疹48例临床观察.上海中医药杂志,2012,46(9)SH. J. TCM Sep.,2012; Vol.46 No.9
[7]张志礼.张志礼皮肤病临床经验辑要[M].北京：中国医药科技出版社,2001：105.
[8]北京中医医院.赵炳南临床经验集.人民卫生出版社,2006,168.
[9]许金华,伶雪梅.中医辨证论治湿疹浅识.辽宁中医药大学学报,2009,11(1)：27.
[10]韩春波.中医辨证治疗湿疹的临床体会.中国医药指南,2012,10(21)：255-256.
[11]宋飞妮,余土根.湿疹的中医辨证治疗.浙江省中医院浙江中西医结合杂志, 2011,21(8)：587-588.
[12]夏吉续,尹军良,杜中.医辨证治疗湿疹88例临床效果观察.吉林医学,2012,1,33(3)605-606.
[13]陈国勤,周兰,周聪和.中医养生法干预对中医辨证治疗慢性湿疹患者影响的研究.光明中医,2012,6,27(6)：1125-1126.
[14]刘爱民,屠远辉,胡会丽,刘爱民.季节、脏腑、经络、部位四位一体辨证治疗湿疹的体会.中国皮肤性病学杂志,2011,25(4)：306-308.
[15]张伟,陈平.马律洗剂湿敷治疗婴儿湿疹105例.中医外治杂志,2010,19(1).
[16]樊梅,林军.苦参荆芥饮湿敷治疗急性湿疹疗效观察.陕西中医,2013,3(7)：861-862.
[17]高淑荣.桑菊饮湿敷治疗婴儿湿疹40例.光明中医,2010,1,25(1).
[18]罗杰,漆永平,喻中明.紫草荆防汤湿敷治疗创伤后湿疹疗效观察.现代中西医结合杂志,2012,21(7).
[20]骆伟雄.双白止瘁洗剂外用治疗慢性手湿疹疗效及实验研究.中国中西医结合皮肤性病学杂志,2012,11,(5)：296-296.
[21]石志刚,梁发波.自拟湿疹灵外洗治疗婴幼儿湿疹临床疗效总结报告.中外医学研究,201 1,1,(93)：49-50.
[22]矫承媛,赵春红,郭丽娟.自制湿疹膏治疗小儿湿疹200例.中医儿科杂志,2009,5(3).
[23]李杰辉,张力,覃文玺.外用自拟吴蛸散治疗慢性湿疹的疗效观察.广西中医药,2011,2,(34)：14-15.
[24]熊玉仙,强仕兴.三黄油剂外用治疗慢性阴部湿疹38例.中国中医药现代远程教育,2010,8(15).
[25]卓鹰,王惠玲,张晖.苦甘方外涂治疗儿童湿疹150例疗效观察.上海中医药杂志,2010,44,(5)SH.J.TCM May,2010,Vo.144 No.5-66.
[26]莫礼滨,磨盘草.艾叶煎液浸浴治疗婴儿湿疹36例.广西中医药2011,10,34(5)： 31.
[27]任朝霞,苗斌,袁秀丽.醋浴方浸浴治疗掌跖皲裂性湿疹43例.陕西中医,2010,31,(12)：1628-1629.
[28]陈建香.中药熏洗治疗湿疹50例.山西中医,2009,25：26-27.
[29]郭新宇,苑艳娟,王延峰.清热除湿汤熏洗治疗肛门急性湿疹53例.河北中医,2010,6,32(6)：843-844.
[30]张蕾.中药熏洗治疗手足皲裂性湿疹.中国民间疗法,2012,20(9)：18.
[31]张纬,罗新媛.中药外用治疗小儿湿疹23例临床观察.国医论坛,2012,3,27(2)：27-28.
[32]刘彩霞.中药内服外洗治疗慢性湿疹60例.环球中医药,2012,6,5(6)：459.
[33]郑赫.中药内服配合湿敷治疗湿疹106例.浙江中医杂志,2010,45(6)
[34]刘韬,何多谊,桑志强.五皮汤外洗方和二妙散联合运用治疗慢性湿疹的疗效观察.宁夏医科大学学报,2012,34(7).
[35]王向义,王敦林,仇裕丰.针灸辨证治疗慢性湿疹65例『J].四川中医,2006,24(9)：109.
[36]谢长才,钟建能,孙建,符文彬.针刺加刺络放血疗法治疗慢性湿疹20例疗效观察[J].新中医,2009,41(2)：90-9.
[37]毕明燕,刘龙壮,林均霞.艾灸治疗顽气固性湿疹32例.上海针灸杂志,2005,24(2).
[38]邓肖英.刺血拔罐结合艾灸治疗急性湿疹46例疗效观察.甘肃中医学院学报,2011,28,(5)53：-54.
[39]杜彩霞,周艳丽.刺血拔罐治疗泛发性湿疹1例.中医研究,2009,5,22(5)：44-45.
[40]潘小霞.多头火针围刺治疗湿疹.中国针灸,2003,23(4).
[41]周震,齐崇玲,李岩.火针结合其他疗法治疗毅裂性湿疹28例.天津中医药,2003,20(2).
[42]徐蓉,高凡桑,李福伦.针药结合治疗亚急性湿疹的随机对照研究.中国中西医结合皮肤性病学杂志,2011,10,(3)：165-166.
[43]林燕,吴春节,曹明璐.慢性湿疹的针灸与中药综合治疗.中国临床医生,2012,40(6)：76-77.
[44]齐庆,李勇,赖宽.西药联合养血祛风法中药复方治疗慢性湿疹的临床疗效观察.中国实用医药,2011,3,6(7)：137-138.
[45]杨娟,史思淑,肖红.中西药合用治疗急性湿疹35例观察及护理实用.中医药杂志,2010,6,26(6)：401-402.
[46]吴学宁,赵红波,葛志明.中药坐浴配合微波理疗治疗肛周湿疹对照观察.实用中医内科杂志,2012,26(10)：55-56.
[47]权亚玲,黄惠玲,高亚梅.紫草油外涂联合红外线照射治疗腹泻所致肛周湿疹的疗效观察.护理管理,2010,25(5)：38-39.
[48]刘焱,方丽.远红外线光谱照射联合外用药治疗婴幼儿湿疹的临床研究.中国妇幼保健,2009,24：4162-4163.
[49]李菁,唐峻.蛇床子汤外用治疗81例皮炎湿疹的临床观察.数理医药学杂志,2009,10,15.
[50]娄方璐,刁庆春,刘毅.湿疹中医外治处方用药规律分析.陕西中医,2012,33(1)：97-98.
[51]吴波.参卿止痒洗液治疗急性湿疹48例.中医外治杂志,2010,20(6).
[52]张子尧.蝉蛇抗敏煎治疗急性湿疹57例临床观察.国医论坛,2007.22(6).
[53]周穗文,陈群雄,岑帼英.复方白鲜皮乳膏的制备及临床应用.中国实用医药,2008,3(11).
[54]陶水仙,张国芳.自制鸡金膏治疗阴囊湿疹56例疗效观察.中国中医药科技,2011,18(1)45.
[55]蔡晓玲,柏倍湿疹散治疗婴儿湿疹疗效观察.中医儿科杂志,2011,7(3)：37-38.
[56]周建芹,刘芳.苦豆子油搽剂治疗小儿湿疹疗效观察.医护论坛,2011,7(6)：163-164.
[57]殷振勇.复方黄柏酊的研制及临床应用.中国实用医药,2011,2,6(5).
[58]陈国勤,周聪和,周兰.利湿止痒片联合消炎止痒霜治疗肛周湿疹临床观察.湖北中医杂志,2009,31(5)：39.
[59]王红,李瑞霞,同军.野菊花治疗新生儿尿布皮炎疗效观察与护理野.护理学杂志,2009,24(5)：53-54.
[60]姜兰香,王爽,庞传超.苦参素治疗老年湿疹的临床疗效.中国老年学杂志,2011,8,(31)：3187-3188.
[61]梁园园,于天浩,陈萍.报黄柏的药理活性及在化妆品中的应用.辽宁中医药大学,2013,15(1)：149-151.
[62]丛欢,李磊.白鲜皮提取物抗湿疹实验研究.中国医学创新,2012,9(12)：18-19.
[63]郝飞,宋志强,钟华.正确认识皮炎湿疹.实用皮肤病学杂志,2010,3,3(1)：18-20.
[64]何玉华,康静,刘光珍等.太原市479名婴幼儿湿疹发病率及相关因素调查[J].中国中医药信息杂,2006,13(8)：12-13.
[65]Oral H1 antihistamines as monotherapy for eczema. Apfelbacher CJ, van Zuuren EJ, Fedorowicz Z, etc.2013 Feb 28;2:CD007770.
[66]Maternal fish consumption during pregnancy and risks of wheezing and eczema in childhood:The Generation R Study. Leermakers ET, Sonnenschein-van der Voort AM, Heppe DH, etc. Eur J Clin Nutr.2013 Feb 27.
[67]Invited commentary:childhood acute lymphoblastic leukemia and allergies:biology or bias?Linabery AM, Spector LG. Am J Epidemiol.2012 Dec 1;176 (11):979-83.
[68]李云涛,朱岩,蔡淑焕.卡介菌多糖核酸注射液治疗慢性湿疹的疗效观察.皮肤病与性病,2002,24(2)：34-35.
[69]Compalati E, Rogkakou A,Passalacqua G. Evidences of efficacy of allergen immunotherapy in atopic dermatitis:an updated review. Curr Opin Allergy Clin Immunol.2012 Aug;12(4):427-33.
[70]柳朋生,郑家润.湿疹治疗的一些最新进展.国外医学皮肤性病学分册,2003,29(1)：58.
[71]Compalati E, Rogkakou A, Passalacqua G. Evidences of efficacy of allergen immunotherapy in atopic dermatitis:an updated review. Curr Opin Allergy Clin Immunol.2012 Aug;12(4):427-33.
[72]黎昌强,陈德宇.湿疹的物理治疗进展.泸卅医学院学报,2008,31(1)：108.
[73]Egawa K.Topical vitamin D3 derivatives in treating hyperkeratotic palmoplantar eczema:a report of five patients[J].J Dermatol.2005,3 2(5):381.
[74]Spring P, Bigliardi P. Chronic hand eczema, conventional and new treatments]. Rev Med Suisse.2012 Apr,48(335):736-8.
[75]黄咏梅.窄谱UVB联合复方氟米松软膏治疗手足慢性湿疹.军医进修学院学报,2010,31(4).
[76]Kerr AC, Ferguson J, Attili SK, Ultraviolet Al phototherapy:a British Photodermatology Group workshop report. Clin Exp Dermatol.2012 Apr,37(3):219-26.
[77]Tan E, Lim D, Rademaker M. Narrowband UVB phototherapy in children:A New Zealand experience. Australas J Dermatol.2010 Nov;51(4):268-73. doi: 10.1111/j.1440-0960.2010.00701. x. Epub 2010, Oct,13.
[78]RolfP Bochmann, Woldemar Seibel, Elke Haase, et al. Externalcompression increases forearm perf us ion [J].J Appl Physiol, 2005, Dec,99 (6):2337.
[79]江向东,黄艳华.量子物理学[M].上海科技教育出版社,2001,81.
[80]李泽春,陈丹,涂春BICOM生物共振治疗慢性湿疹的观察及护理.护士进修杂志,2011,26,(20)：1857-1858.
[81]RolfP Bochmann, Woldemar Seibel, Elke Haase, et al.Externalcompression increases forearm perfusion [J]. J Appl Physiol,2005, Dec,99 (6):2337.
[2]Ui H, Andoh T, Lee JB, etal. Potent pruritogenic action of tryptase mediated by PAR-2 receptor and its involvement on anti-pruritic effect of nafamostat mesilate in mice[J]. Eur J Pharmacol,2006,530 (1-2):172-178.
[3]邓小元.皮肤源性瘙痒发生机制的研究进展.中国医药科学,2012,2(3)：42-43其中组胺是公认的、经典的致痒介质.
[4]Segre JA. Epidermal barrier formation and recovery in skin disorders[J]. ClinInvest,2006,116(5):1150-1158.
[5]Katoh M, Hamajima F,Ogasawara T, etal. Assessment of human epidermal model LabCyte EPI-MODEL for in vitro,skin irritation testing according to European Centre for the Validation of Alternative Methods(ECVAM)-validate protocol[J]. Toxicol Sci,2009,34(3):327-334.
[6]张芳芳,车雅敏,傅志宜Filaggrin和Caspase-14在皮肤屏障中的作用.皮肤性病诊疗学杂志,2012,19(2)：114-117.
[7]Ballardini N, Kull I, Soderhall C, Eczema severity in preadolescent children and its relation to sex, filaggrin mutations, asthma, rhinitis, aggravating factors and topical treatment:a report from the BAMSE birth cohort. Br J Dermatol.2013 Mar;168(3):588-94.
[8]Sandilands A, Sutherland C, Irvine AD, etal. Filaggrin in the frontline: role in skin barrier function and disease[J]. Cell Sci,2009, 22(9):1285-1294.
[9]Eckhart L, Ban J,Fischer H, etal. Caspase-14:analysis of gene structure and mRNA expression during keratinocyte differentiation[J]. Biochem Biophys Res Commun,2000,277(3):655-659.
[10]Alibardi L, Tschachler E, Eckhart L. Distribution of Caspase-14 in epidermis and hair follicles is evolutionarilyconserved among mammals[J].Anat Rec A Discov Mol Cell Evol Biol,2005,286(2):962-973.
[11]李军,吴伽.一氧化氮对小鼠皮肤Caspase-14表达及屏障功能的影响.吉林医学,201莹31(10)：1301-1302.
[12]Denecker G, Hoste E, Gilbert B, et al. Caspase-14 protects against epidermal UVB photo damage and water loss[J]. Nat Cell Biol,2007,9(4):666.
[13]Landeck L, Visser M, Kezic S, Genotype-phenotype associations in filaggrin loss-of-function mutation carriers. Contact Dermatitis.2013 Mar; 68 (3):149-55.
[14]Thyssen JP, Linneberg A, Ross-Hansen K. Filaggrin mutations are strongly associated with contact sensitization in individuals with dermatitis.Contact Dermatitis.2013, Jan;24.
[15]Segre JA. Epidermal barrier formation and recovery in skin dis-orders[J].J Clin Invest,2006,116:1150-1158.
[16]董海胜,陈斌.茶树籽油与马齿苋籽油中脂肪酸及维生素E组成分析.营养学报,2012,34(1)：94-95.
[17]起珏,庞勤,涂颖.含马齿苋、青刺果提取物护肤品对激素依赖性皮炎辅助治疗作用的临床观察.2011全国中西医结合皮肤性病学术会议论文汇编.
[18]Marly JP. NMF and cosmetology of cutaneous hydration [J]. Ann Derm atol Venereol,2002,129:131-136.
[19]Nakagawa N, Sakai S, Matsumoto M, etal. Relationship between NMF (lactate and potassium)content and the physical properties of the stratum corneum in healthy subjects [J].J Invest Dermatol,2004,122:755-763.
[20]杨怀珠.雷公藤多贰对湿疹患者T淋巴细胞亚群的影响.中国中西医结合杂志,2007,27(7)：592.
[21]宋瑜,李咏梅,马绍尧,等.除湿止痒合剂对变应性接触性皮炎小鼠模型.Thl／Th2相关细胞因子水平的影响仁[T].成都中医药大学学报,2008,31(1)：32-3.
[22]Drago L, Toscano M, De Vecchi E, Changing of fecal flora and clinical effect of L. salivarius LS01 in adults with atopic dermatitis.J Clin Gastroenterol.2012 Oct,46 Suppl:S56-63.
[23]Sabin BR, Peters N, Peters AT. Chapter 20:Atopic dermatitis. Allergy Asthma Proc.2012 May-Jun;33.
[24]邢艳玲,张玉环,许建.工P--10对变应性接触性皮炎小鼠Thl／ThZ型细胞因子表达的影响[J].天津医科大学学报,2008,14(4)：492-494.
[25]王远红,王相明,等.中西结合治疗湿疹近况[J].黑龙江中医药,2009,38(3)：54-56.
[26]王远红,焦彦民,孙惠敏.除湿饮治疗湿疹(湿热型)的临床观察及对血清TNF-a水平的影响.黑龙江中医药,2011,(5)：28.
[27]吴华勋,魏伟.Th22细胞在炎症免疫性疾病中作用的研究进展.免疫学杂志,2012,28(9)：816-817.
[28]Fujita H. The role of IL-22 in the pathogenesis of skin diseases. Nihon Rinsho Meneki Gakkai Kaishi.2012,35(3):168-75.
[29]吕晶,徐军发.Th22细胞：一种新型的辅助型T细胞.中国热带医学,2011.11(1).
[30]Eyerich S, Eyerich K, Pennino D, etal. Th22 cellsrepresent a distinct human T cell subset involved inepidermalimmunity and remodeling[J]. J Clin Invest,2009,119(12):3573-3585.
[31]Stefanie E, Kilian E, Davide P, etal. Th22 cells represent a distinct human T cell subset involved in epidermal immunity and remodel ing [J]. J. Clin.Invest,2009,119:3573-3585.
[32]Kerstan A, Brocker EB, Trautmann A. Decisive role of tumor necrosis factor-a for spongiosis formation in acute eczematous dermatitis. Arch Dermatol Res.2011 Nov;303(9):651-8.
[33]Ngatu NR, Okajima MK, Yokogawa M, Anti-allergic effects of Vernonia amygdalina leaf extracts in hapten-induced atopic dermatitis-like disease in mice. Allergol Int.2012 Dec;61(4):597-607.
[34]van Hamburg JP, Corneth OB, Paulissen SM. IL-17/Th17 mediated synovial inflammation is IL-22 independent. Ann Rheum Dis.2013, Jan 17.
[35]NogralesKE, ZabaLC, ShemerA, etal. IL-22-produeing "T22" TeellsaeeountforuPregulatedIL-22inatopiedermatitisdespiteredueed IL-17 rodueing TH17 Teells. JA11. rgyClinInununol.2009,123(6):1244-52.
[36]Jabbari A, Johnson-Huang LM, Krueger JG. Role of the immune system and immunological circuits in psoriasis.G Ital Dermatol Venereol.2011 Feb; 146(1):17-30.
[37]Ring J, Mohrenschlager M, Weidinger S. Molecular genetics of atopic eczema. Chem Immunol Allergy.2012,96:24-9.
[38]Rebane A, Zimmermann M, Aab A. Mechanisms of IFN-γ-induced apoptosis of human skin keratinocytes in patients with atopic dermatitis. J Allergy Clin Immunol.2012 May;129(5):1297-306.
[39]Leung DY,Gao PS, Grigoryev DN,Human atopic dermatitis complicated by eczema herpeticum is associated with abnormalities in IFN-γ response. J Allergy Clin Immunol.2011 Apr;127(4):965-73.
[40]Werfel T.The role of leukocytes, keratinocytes, and allergen-speciIgE in the development of atopic dermatitis [J]. J Invest Dermatol,2009,129(8):1878-91.
[41]Heninger E, Falus A, Darvas Z, etal. Both interferon (IFN) alpha and IFN gamma inhibit histidine decarboxylase expression in the HT168 human melanoma cell line. Inflammation Res,2000,49(8):393-397.
[42]张玉环,陈保疆,焦振山,等.特应性皮炎患者单个核细胞Thl/Th2和IFN-Y及IL-4 mRNA表达[J].天津医药,2006,34(12)：833-834.
[43]Yang XY, Wang HY, Zhao XY,Th22, but not Th17 Might be a Good Index to Predict the Tissue Involvement of Systemic Lupus Erythematosus. J Clin Immunol.2013 Feb,23.
[44]KarinM. Nuclearfaetor-kaPPaBincaneerdeveloPmentandProgression[J]. N atural,2006,441(7092):431-436.
[45]Jennifer L, Katia B, Rene de Waal M.Development and function of TH17 cells in health and disease[J]. J Allergy ClinImmunol,2009,123:1004-1011.
[46]陆丽明,程楷涛.湿疹发生机理的研究进展.亚太传统医 药,2007,3(9)：76-78.
[47]Wang H, Mu W, Ja Q, Cytokine Profile of a Self-Healing Fonsecaea pedrosoi Infection in Murine Model.Cell Biochem Biophys.2013 Mar,12.
[48]Ali R, Kumar S, Naqvi RA, B and T cell epitope mapping and study the humoral and cell mediated immune response to B-T constructs of YscF antigen of Yersinia pestis. Comp Immunol Microbiol Infect Dis.2013
[49]李丽,甄莉.黄芪对湿疹大鼠皮损中白细胞介素-4和干扰素-Y影响的实验研究.中国药物与临床,2010,10(1).
[50]林星游,王炎,翁雪云.湿疹的中医辨治探析.中国当代医药,2009,16(11).
[51]罗杰,漆永平,喻中明.紫草荆防汤湿敷治疗创伤后湿疹疗效观察.现代中西医结合杂志,2012,21(7)：721-722.
[52]Epidemiology of human atopic dermatitis--seven areas of notable progress and seven areas of notable ignorance. Williams HC. Vet Dermatol. 2013 Feb,24(1):3-9.
[53]张振奎.婴儿湿疹情况调查分析[J].医药论坛杂志,2006,27(2)：42.
[54]Oral H1 antihistamines as monotherapy for eczema. Apfelbacher CJ, van Zuuren EJ, Fedorowicz Z, etc.2013 Feb,28(2):CD007770.
[55]Maternal fish consumption during pregnancy and risks of wheezing and eczema in childhood:The Generation R Study. Leermakers ET, Sonnenschein-van der Voort AM, Heppe DH, etc. Eur J Clin Nutr.2013 Feb,27.
[56]Invited commentary:childhood acute lymphoblastic leukemia and allergies:biology or biasL inabery AM, Spector LG. Am J Epidemiol.2012 Dec 1,176(11):979-83.
[57]Thyssen JP, Johansen JD, Zachariae C, Xerosis is Associated with Atopic Dermatitis, Hand Eczema and Contact Sensitization Independent of Filaggrin Gene Mutations.Acta Derm Venereol.2013, Feb,18.
[58]匡玉珍,刘志军,段明.衡阳地区荨麻疹和湿疹患者过敏原检测分析.南华大学学报(医学版),2叭0,38(2)：236-238.
[59]Kang K, Stevens SR. Pathophysiology of atopic dermatitis[J]. ClinDermatol,2003,21:116-121.
[60]盛楠,宗文凯,余美文,等.咪唑斯汀治疗湿疹和特应性皮炎临床疗效及免疫机制的初步研究[J].临床皮肤科杂志,2009,38(7)：440-442.
[61]李邻峰.微生物变态反应与微生物性湿疹[J].岭南皮肤性病科杂志,2009,16(4)：211-212.
[62]DahlMV. Clinical demato logy[M].3rded. Mosby Si Louis,1996,20.
[63]Wehner J, Neuber K. Staphy Lococcus aureus enterotoxins inducehistam in e and leukotrien e release in patients with atopic eczema[J].B r J Dem ato,2001,145(2):302-305.
[64]赵凤臣,吴洪军,潘高荣.马齿苋栽培技术.中国林副特产,2002,63(4)：11.
[65]李秀花,高志花,靳玲品.马齿苋的营养及应用.畜牧与兽医,2006,38(8)：34-35.
[66]黄海辉.感染性腹泻的经验治疗[J].中国临床医生,2002,30(7)：5-6
[67]侯俊然,范迎.马齿苋体外抗感染性腹泻菌群的作用研究.中外医疗,2010,28：29.
[68]张秀娟,季宇彬,曲中原,等.马齿苋体外抗茵作用的实验研究.中国微生态学杂志,2002,14(5)：277-279.
[69]李晓,冯雷,胡尊丽.马齿苋多糖的抗氧化活性研究.中国生化药物杂志,2010,31(4)：244-245.
[70]Lee AS, Kim JS, Lee YJ, Kang DG,Anti-TNF-a Activity of Portulaca oleracea in Vascular Endothelial Cells. Int J Mol Sci.2012; 13(5):5628-44.
[71]向兰,邢东明,王伟.马齿苋的化学成分研究进展.亚太传统医药,2006,7：64-68.
[72]崔曼,尹苗,安利国.马齿苋多糖的抗肿瘤活性.山东师大学报(自然科学版),2002,17(1)：73-75.
[73]杨桂芹,王长泉.马齿苋甜菜红素抗肿瘤实验研究.时珍国医国药,2010,21(2)：388-389.
[74]史仁玖,苗苗,李艳玲.不同生长期马齿苋ω-3脂肪酸及矿物质元素含量分析.营养学报,2010,32(4)：404-406.
[75]Wanyin W, Liwei D, Lin J, etc. Ethanol extract of Portulaca oleracea L. protects against hypoxia-induced neuro damage through modulating endogenous erythropoietin expression. J Nutr Biochem.2012,23(4):385-91.
[76]余南才,黄浩,肖宏.马齿苋水提液抗D-半乳糖诱导的脑神经毒性损伤作用机制.科技成果,2009.
[77]康洁.马齿苋多糖对胎鼠大脑皮层细胞保护作用的研究.现代预防医学,2010,37(4)：747-748.
[78]高红梅,陈新,王月珍.马齿苋降血糖有效部位研究.长春中医药大学学报,2012,28(3)：536.
[79]Shobeiri SF, Sharei S, Heidari A. Portulaca oleracea L. in the treatment of patients with abnormal uterine bleeding:a pilot clinical trial.Phytother Res.2009 Oct,23(10):1411-4.
[80]金英子,张红英,付柳静,等.马齿苋提取物的抗炎消肿及镇痛作用研究[J].延边大学医学学报,2008,31(4)：259-260.
[81]王国玉,王浩字,佟继铭.马齿苋的化学成分与药理作用研究现状.承德医学院学报,2012,29(1)：82-84.
[82]朱丽,徐为公,赵广荣.马齿苋的研究现状与综合开发利用.北林果研究,2006,21(2).
[83]朱晓宦,吴向阳,仰榴青.马齿苋的化学成分和药理活性研究进展.常熟理工学院学报(自然科学版),2007,21(4).
[84]Y. P. Shi, Yan-Ping Sh, i Juan Chen, etc. Determination ofnoradrenaline and dopamine in Chinese herbalextracts from Por-tulaca oleracea L by high-performanceliquid chromatography[J]. ChromatographyA,2003,1003: 127-132.
[85]李湘力,黄鸣清,李万瑶.马齿苋治疗蜂毒过敏反应的实验研究[J].海峡药学,2007,19(6)：39-41.
[86]陆霄鹤,等.马齿苋及鱼腥草水提取液体外抑菌实验.抗感染药学,2010,7(1).
[87]杨柳,等.马齿苋提取物体外清除自由基活性的研究.药学服务与研究, 2011,11(3).
[88]董立巍,王万银,岳义田.马齿苋总黄酮抗小鼠缺氧作用及其机制研究.中西医结合学报,2005,3(6).
[89]佘鸿燕,王见宾,刘福来.复方马齿苋溶液治疗婴儿急性湿疹的临床对比研究.时珍国医国药,2007,18(12).
[90]马玲,李月红,刘红霞.除湿解毒汤治疗湿热型急性湿疹的疗效及对血清总IgE水平的影响.新疆医科大学学报,2010,33(12).
[91]李元红.复方黄柏液皮肤科新用[J].中国中医急诊,2006,15(6)：627-672.
[92]魏红英,刘景桢.复方黄柏液治疗婴儿湿疹疗效观察.皮肤病与性病,2011,33(4).
[93]曾晓会,杜铁良,陈玉兴.小儿湿疹软膏对动物接触性湿疹模型的影响.江西中医学院学报,2012,2,24(1)：50-51.
[94]Man M Q,Hupe M, Sun R, Topical apigenin alleviates cutaneous inflammation in murine models. Evid Based Complement Alternat Med. 2012,2012:912028.
[95]于均峰,朱启星.三氯乙烯引起BALB/c裸鼠刺激性接触性皮炎作用机制的研究.中国职业医学,2009,2,36(1).
[96]Stewart I,Seawright AA, Schluter PJ, Primary irritant and delayed-contact hypersensitivity reactions to the freshwater cyanobacterium Cylindrospermopsis raciborskii and its associated toxin cylindrospermopsin. BMC Dermatol.2006 Mar 31(6):5.
[97]宗桂珍,王冰,李德凤.系统性光变态反应检测动物模型的建立.中国比较医学杂志,2009,19(1)：22-24.
[98]Greenspoon J, Ahluwalia R, Juma N, Allergic and photoallergic contact dermatitis:a 10-year experience. Dermatitis.2013 Jan-Feb;24(1):29-32.
[99]Conti R, Bassi A, Difonzo EM, A case of photoallergic contact dermatitis caused by unusual exposure to ketoprofen. Dermatitis.2012 Nov-Dec;23(6):295-6.
[100]李秀芳,金若敏,符胜光.2种品系大鼠被动皮肤过敏反应的差异性比较.中 国实验方剂学杂志,2010,16(10)：102-103.
[101]Cho IH, Lee MJ, Kim JH, Fritillaria ussuriensis extract inhibits the production of inflammatory cytokine and MAPKs in mast cells. Biosci Biotechnol Biochem.2011;75(8):1440-5.
[102]Kim SR, Choi HS, Seo HS. Topical application of herbal mixture extract inhibits ovalbumin- or 2,4-dinitrochlorobenzene-induced atopic dermatitis. Evid Based Complement Alternat Med.2012:545497.
[103]马松阳,薛东运.应用1%吡美莫司乳膏治疗遗传性过敏性皮炎临床分析.中国社区医师·医学专业,2010,22(12)：247.
[104]张玉锁.抗过敏汤治疗急性湿疹的实验研究.中华中医药学刊,2008,26(12).
[105]林清,张建军,李伟.五倍子与黄柏外用治疗急性湿疹的配伍合理性研究.中华中医药杂志(原中国医药学报),2011,26(9).
[106]芦源.小鼠背部慢性皮炎一湿疹模型的初步研究.辽宁中医药大学学报,2009,11(10).
[107]王国江,顾军.小鼠背部变应性接触性皮炎模型的建立[J].中国职业医学,2004,31(1)：17-19.
[1]徐晶钰,张璇,魏品康.中医药治疗湿疹合并抑郁症思路[J].中国中医药信息杂志,2011,8(10)：95.
[2]刘纯莉,李红军,尹东辉.活血化瘀法治疗慢性湿疹综述[J].北京中医药大学学报：中医临床版,2011,18(1)：44-45.
[3]胡赞,朱国强.湿疮病辨治及用药心得.光明中医,2012,2,27(2):CJGMCM February 2012. Vol27.2.
[4]李咏梅,宋瑜,马绍尧.脏腑辨证治疗湿疹265例临床观察[J].浙江中西医结合杂志,2004,14(9)：547-549.
[5]赵丽平.中医辨证论治湿疹86例[J].中国中医药信息杂志,2000,7(7)：66-67.
[6]宋瑜,马绍尧,李咏梅.健脾除湿方治疗湿疹48例临床观察.上海中医药杂志,2012,46(9)SH. J. TCM Sep.,2012; Vol.46 No.9
[7]张志礼.张志礼皮肤病临床经验辑要[M].北京：中国医药科技出版社,2001：105.
[8]北京中医医院.赵炳南临床经验集.人民卫生出版社,2006,168.
[9]许金华,伶雪梅.中医辨证论治湿疹浅识.辽宁中医药大学学报,2009,11(1)：27.
[10]韩春波.中医辨证治疗湿疹的临床体会.中国医药指南,2012,10(21)：255-256.
[11]宋飞妮,余土根.湿疹的中医辨证治疗.浙江省中医院浙江中西医结合杂志, 2011,21(8)：587-588.
[12]夏吉续,尹军良,杜中.医辨证治疗湿疹88例临床效果观察.吉林医学,2012,1,33(3)605-606.
[13]陈国勤,周兰,周聪和.中医养生法干预对中医辨证治疗慢性湿疹患者影响的研究.光明中医,2012,6,27(6)：1125-1126.
[14]刘爱民,屠远辉,胡会丽,刘爱民.季节、脏腑、经络、部位四位一体辨证治疗湿疹的体会.中国皮肤性病学杂志,2011,25(4)：306-308.
[15]张伟,陈平.马律洗剂湿敷治疗婴儿湿疹105例.中医外治杂志,2010,19(1).
[16]樊梅,林军.苦参荆芥饮湿敷治疗急性湿疹疗效观察.陕西中医,2013,3(7)：861-862.
[17]高淑荣.桑菊饮湿敷治疗婴儿湿疹40例.光明中医,2010,1,25(1).
[18]罗杰,漆永平,喻中明.紫草荆防汤湿敷治疗创伤后湿疹疗效观察.现代中西医结合杂志,2012,21(7).
[20]骆伟雄.双白止瘁洗剂外用治疗慢性手湿疹疗效及实验研究.中国中西医结合皮肤性病学杂志,2012,11,(5)：296-296.
[21]石志刚,梁发波.自拟湿疹灵外洗治疗婴幼儿湿疹临床疗效总结报告.中外医学研究,201 1,1,(93)：49-50.
[22]矫承媛,赵春红,郭丽娟.自制湿疹膏治疗小儿湿疹200例.中医儿科杂志,2009,5(3).
[23]李杰辉,张力,覃文玺.外用自拟吴蛸散治疗慢性湿疹的疗效观察.广西中医药,2011,2,(34)：14-15.
[24]熊玉仙,强仕兴.三黄油剂外用治疗慢性阴部湿疹38例.中国中医药现代远程教育,2010,8(15).
[25]卓鹰,王惠玲,张晖.苦甘方外涂治疗儿童湿疹150例疗效观察.上海中医药杂志,2010,44,(5)SH.J.TCM May,2010,Vo.144 No.5-66.
[26]莫礼滨,磨盘草.艾叶煎液浸浴治疗婴儿湿疹36例.广西中医药2011,10,34(5)： 31.
[27]任朝霞,苗斌,袁秀丽.醋浴方浸浴治疗掌跖皲裂性湿疹43例.陕西中医,2010,31,(12)：1628-1629.
[28]陈建香.中药熏洗治疗湿疹50例.山西中医,2009,25：26-27.
[29]郭新宇,苑艳娟,王延峰.清热除湿汤熏洗治疗肛门急性湿疹53例.河北中医,2010,6,32(6)：843-844.
[30]张蕾.中药熏洗治疗手足皲裂性湿疹.中国民间疗法,2012,20(9)：18.
[31]张纬,罗新媛.中药外用治疗小儿湿疹23例临床观察.国医论坛,2012,3,27(2)：27-28.
[32]刘彩霞.中药内服外洗治疗慢性湿疹60例.环球中医药,2012,6,5(6)：459.
[33]郑赫.中药内服配合湿敷治疗湿疹106例.浙江中医杂志,2010,45(6)
[34]刘韬,何多谊,桑志强.五皮汤外洗方和二妙散联合运用治疗慢性湿疹的疗效观察.宁夏医科大学学报,2012,34(7).
[35]王向义,王敦林,仇裕丰.针灸辨证治疗慢性湿疹65例『J].四川中医,2006,24(9)：109.
[36]谢长才,钟建能,孙建,符文彬.针刺加刺络放血疗法治疗慢性湿疹20例疗效观察[J].新中医,2009,41(2)：90-9.
[37]毕明燕,刘龙壮,林均霞.艾灸治疗顽气固性湿疹32例.上海针灸杂志,2005,24(2).
[38]邓肖英.刺血拔罐结合艾灸治疗急性湿疹46例疗效观察.甘肃中医学院学报,2011,28,(5)53：-54.
[39]杜彩霞,周艳丽.刺血拔罐治疗泛发性湿疹1例.中医研究,2009,5,22(5)：44-45.
[40]潘小霞.多头火针围刺治疗湿疹.中国针灸,2003,23(4).
[41]周震,齐崇玲,李岩.火针结合其他疗法治疗毅裂性湿疹28例.天津中医药,2003,20(2).
[42]徐蓉,高凡桑,李福伦.针药结合治疗亚急性湿疹的随机对照研究.中国中西医结合皮肤性病学杂志,2011,10,(3)：165-166.
[43]林燕,吴春节,曹明璐.慢性湿疹的针灸与中药综合治疗.中国临床医生,2012,40(6)：76-77.
[44]齐庆,李勇,赖宽.西药联合养血祛风法中药复方治疗慢性湿疹的临床疗效观察.中国实用医药,2011,3,6(7)：137-138.
[45]杨娟,史思淑,肖红.中西药合用治疗急性湿疹35例观察及护理实用.中医药杂志,2010,6,26(6)：401-402.
[46]吴学宁,赵红波,葛志明.中药坐浴配合微波理疗治疗肛周湿疹对照观察.实用中医内科杂志,2012,26(10)：55-56.
[47]权亚玲,黄惠玲,高亚梅.紫草油外涂联合红外线照射治疗腹泻所致肛周湿疹的疗效观察.护理管理,2010,25(5)：38-39.
[48]刘焱,方丽.远红外线光谱照射联合外用药治疗婴幼儿湿疹的临床研究.中国妇幼保健,2009,24：4162-4163.
[49]李菁,唐峻.蛇床子汤外用治疗81例皮炎湿疹的临床观察.数理医药学杂志,2009,10,15.
[50]娄方璐,刁庆春,刘毅.湿疹中医外治处方用药规律分析.陕西中医,2012,33(1)：97-98.
[51]吴波.参卿止痒洗液治疗急性湿疹48例.中医外治杂志,2010,20(6).
[52]张子尧.蝉蛇抗敏煎治疗急性湿疹57例临床观察.国医论坛,2007.22(6).
[53]周穗文,陈群雄,岑帼英.复方白鲜皮乳膏的制备及临床应用.中国实用医药,2008,3(11).
[54]陶水仙,张国芳.自制鸡金膏治疗阴囊湿疹56例疗效观察.中国中医药科技,2011,18(1)45.
[55]蔡晓玲,柏倍湿疹散治疗婴儿湿疹疗效观察.中医儿科杂志,2011,7(3)：37-38.
[56]周建芹,刘芳.苦豆子油搽剂治疗小儿湿疹疗效观察.医护论坛,2011,7(6)：163-164.
[57]殷振勇.复方黄柏酊的研制及临床应用.中国实用医药,2011,2,6(5).
[58]陈国勤,周聪和,周兰.利湿止痒片联合消炎止痒霜治疗肛周湿疹临床观察.湖北中医杂志,2009,31(5)：39.
[59]王红,李瑞霞,同军.野菊花治疗新生儿尿布皮炎疗效观察与护理野.护理学杂志,2009,24(5)：53-54.
[60]姜兰香,王爽,庞传超.苦参素治疗老年湿疹的临床疗效.中国老年学杂志,2011,8,(31)：3187-3188.
[61]梁园园,于天浩,陈萍.报黄柏的药理活性及在化妆品中的应用.辽宁中医药大学,2013,15(1)：149-151.
[62]丛欢,李磊.白鲜皮提取物抗湿疹实验研究.中国医学创新,2012,9(12)：18-19.
[63]郝飞,宋志强,钟华.正确认识皮炎湿疹.实用皮肤病学杂志,2010,3,3(1)：18-20.
[64]何玉华,康静,刘光珍等.太原市479名婴幼儿湿疹发病率及相关因素调查[J].中国中医药信息杂,2006,13(8)：12-13.
[65]Oral H1 antihistamines as monotherapy for eczema. Apfelbacher CJ, van Zuuren EJ, Fedorowicz Z, etc.2013 Feb 28;2:CD007770.
[66]Maternal fish consumption during pregnancy and risks of wheezing and eczema in childhood:The Generation R Study. Leermakers ET, Sonnenschein-van der Voort AM, Heppe DH, etc. Eur J Clin Nutr.2013 Feb 27.
[67]Invited commentary:childhood acute lymphoblastic leukemia and allergies:biology or bias?Linabery AM, Spector LG. Am J Epidemiol.2012 Dec 1;176 (11):979-83.
[68]李云涛,朱岩,蔡淑焕.卡介菌多糖核酸注射液治疗慢性湿疹的疗效观察.皮肤病与性病,2002,24(2)：34-35.
[69]Compalati E, Rogkakou A,Passalacqua G. Evidences of efficacy of allergen immunotherapy in atopic dermatitis:an updated review. Curr Opin Allergy Clin Immunol.2012 Aug;12(4):427-33.
[70]柳朋生,郑家润.湿疹治疗的一些最新进展.国外医学皮肤性病学分册,2003,29(1)：58.
[71]Compalati E, Rogkakou A, Passalacqua G. Evidences of efficacy of allergen immunotherapy in atopic dermatitis:an updated review. Curr Opin Allergy Clin Immunol.2012 Aug;12(4):427-33.
[72]黎昌强,陈德宇.湿疹的物理治疗进展.泸卅医学院学报,2008,31(1)：108.
[73]Egawa K.Topical vitamin D3 derivatives in treating hyperkeratotic palmoplantar eczema:a report of five patients[J].J Dermatol.2005,3 2(5):381.
[74]Spring P, Bigliardi P. Chronic hand eczema, conventional and new treatments]. Rev Med Suisse.2012 Apr,48(335):736-8.
[75]黄咏梅.窄谱UVB联合复方氟米松软膏治疗手足慢性湿疹.军医进修学院学报,2010,31(4).
[76]Kerr AC, Ferguson J, Attili SK, Ultraviolet Al phototherapy:a British Photodermatology Group workshop report. Clin Exp Dermatol.2012 Apr,37(3):219-26.
[77]Tan E, Lim D, Rademaker M. Narrowband UVB phototherapy in children:A New Zealand experience. Australas J Dermatol.2010 Nov;51(4):268-73. doi: 10.1111/j.1440-0960.2010.00701. x. Epub 2010, Oct,13.
[78]RolfP Bochmann, Woldemar Seibel, Elke Haase, et al. Externalcompression increases forearm perf us ion [J].J Appl Physiol, 2005, Dec,99 (6):2337.
[79]江向东,黄艳华.量子物理学[M].上海科技教育出版社,2001,81.
[80]李泽春,陈丹,涂春BICOM生物共振治疗慢性湿疹的观察及护理.护士进修杂志,2011,26,(20)：1857-1858.
[81]RolfP Bochmann, Woldemar Seibel, Elke Haase, et al.Externalcompression increases forearm perfusion [J]. J Appl Physiol,2005, Dec,99 (6):2337.