微波辅助合成用于开管毛细管电色谱柱的制备及应用性能的研究
|
摘要
开管毛细管电色谱作为毛细管电色谱的重要分支,结合了毛细管区带电泳的高柱效和高效液相色谱高选择性的优点,成为当今分析化学领域的研究热点。大量的末端官能团、紧密且精确控制的分子结构是树状大分子所具有的独特性质,PAMAM是通过支化基元逐步重复的反应得到的具有树枝状高度支化结构的大分子,将其引入到毛细管电色谱柱中可以大大增加柱的表面积、容量和选择性,提升柱的性能。微波加热作为一种不同于传统加热模式,具有内外同热、热应力小、效率高、加热速度快的特点。本文将开管毛细管电色谱柱的制备技术,PAMAM树枝状大分子和微波辅助合成方法相结合,开展以下研究工作。
采用微波辅助合成方法快速地制备了PAMAM树枝状大分子为固定相的开管毛细管电色谱柱。在微波加热的条件下,通过丙烯酸甲酯与KH 550的端氨基发生Micheal加成反应,得到半代的PAMAM,之后端酯基与乙二胺发生酰胺化反应,得到一代的枝形大分子PAMAM,重复以上,制得二代、三代树枝状大分子修饰的毛细管开管电色谱柱。首次尝试用FITC荧光标记的方法对制得的PAMAM修饰的开管毛细管电色谱柱进行表征,结果表明微波辅助合成方法用于制备PAMAM树枝状大分子修饰毛细管柱是可行的。与常规方法相比,微波辅助合成方法大大地缩短了PAMAM/OTCEC柱的制备周期。获得的3G PAMAM/OTCEC柱对丙氨酸和辅氨酸达到了基线分离。用丙酮考察获得PAMAM/OTCEC柱,迁移时间的RSD＜3.O%,说明制备的毛细管柱具有良好的重现性。用丙氨酸和辅氨酸对3 GPAMAM/OTCEC柱的稳定性进行测试,10天后峰面积RSD＜6.0%,说明制得的开管毛细管色谱柱的具有良好的稳定性。
利用微波辅助合成方法制备β-环糊精衍生物(Br-β-CD)为固定相的开管毛细管电色谱柱。通过FITC荧光标记的方法优化KH 550修饰毛细管柱的微波反应时间,在微波功率140 W,微波温度40℃的条件下,微波反应10 min,KH 550修饰毛细管柱的荧光强度最大。继续通入Br-β-CD,微波反应30 min,制得Br-β-CD/OTCEC柱。获得的毛细管柱对N-乙酰-DL-蛋氨酸、l-苯基-1,2-乙二醇达到基线分离。用丙酮和1-苯基-1,2-乙二醇考察制得毛细管柱的性能,实验结果表明,制得的Br-β-CD/OTCEC柱具有良好的稳定性和重现性。
毛细管酶微反应器是从固定化酶术发展而来的,将酶固定在毛细管内,可以进一步减少酶的消耗量。本文以氨基酰化酶为模型,成功地制备3G PAMAM树枝分子修饰的毛细管柱酶微反应器。通过高分支PAMAM树枝状大分子的引入,可以大大增加表面固定酶的容量,并且减小固定化的酶分子在刚性基体表面出现构像变化的可能性,保持酶的活性。利用毛细管区带电泳(CZE)优化制备的酶微反应器的酶解条件,实验结果表明,酶解温度37℃,17.5μg mL~(-1)N-乙酰-DL-蛋氨酸磷酸缓冲溶液(pH7.5,0.02 moL L~(-1)),底物的流速4μL min~(-1),制备的毛细管酶微反应器具有良好的酶解效果。在优化条件下,考察10天内(每天酶解5次)酶解产物峰面积的变化。实验结果表明,10天后酶解产物的峰面积的RSD<10%,说明制备的毛细管柱酶微反应器具有良好的稳定性。
β-环糊精柱撑水滑石(Mg_2Al/SO_3-β-CD/LD)是一种新型有机/无机杂化的纳米材料。本文以Mg_2Al/SO_3-β-CD/LDH为固定相,制备水滑石内壁修饰的毛细管电色谱开管柱,获得的毛细管柱在pH8.0Tris-HCl缓冲溶液中对1-苯基-1,2-乙二醇达到基线分离(Rs=3.03)。
Open tubular capillary electrochromatography(OTCEC) is one of the most important approaches in capillary electrochromatography(CEC). Microwave irradiation method has become increasingly popular for modern chemical synthesis strategies sine.it's first application towards organic chemistry.Compared with conventional synthesis process, microwave irradiation method has the advantages such as,heating system with uniform temperature,selective heating and no hysteresis effect. Polyamidoamine(PAMAM) dendrimers stationary phase for OTCEC was prepared from silanized fused-silica capillaries by 3-Aminopropylmethyldimethoxysilane(KH 550) with repeating tertiary amine/amide branching units using microwave-assisted method. Fluorescein isothiocyanate(FITC) is one of the most commonly used fluorescent dyes which act as an amine reactive label in bioassay.FITC was used as a label for the characterization of PAMAM dendrimer modified capillary column.The results verified that the preparation of PAMAM dendrimer modified column with microwave irradiation is feasible.Comparing with the conventional synthesis,microwave irradiation significantly improved the reaction speed and shortened the preparation time.The separation of Alanine and Proline was demonstrated with prepared PAMAM/OTCEC column.The results showed that the separation was gradually improved with the increasing of PAMAM generation,and the baseline separation can be obtained by G3 PAMAM dendrimer modified capillary column.The reproducibility of the PAMAM/OTCEC column was evaluated by measuring the RSD of the migration time.The results showed that the reproducibility of the column was satisfactory(RSD＜3.0%).The rate of column efficiency decline was used to examine the stability of G3 PAMAM/OTCEC column. Alanine and Proline was used to determine the stability for consecutive 10 days,the results showed that the columns have a good stability.
At present,cyclodextrins(CDs) and their derivatives are the most widely applied chiral selector due to its low price,low UV absorbance, and unique structure.In this study,FITC was used as label for the characterization of KH 550 modified capillary column prepared by microwave assisted method.The results verified that the preparation of KH 550 modified column with microwave irradiation is feasible. Bromoacetate-substituted-β-cyclodextrins(Br-β-CD) have been successfully bonded to KH 550 modified column as chiral stationary phase in OTCEC.Comparing with the conventional synthesis,microwave assisted process significantly improved the reaction speed and shortened the preparation time from 16h to 40min.Baseline chiral separation of N-acetyl-DL-methionine and 1-phenyl-1,2-ethanediol was obtained in Br-β-CD modified column.The reproducibility of the Br-β-CD/OTCEC column was evaluated by measuring the RSD of the migration times of chiral compound 1-phenyl-1,2-ethanediol.The migration time data indicated that the reproducibility of the column was satisfactory(RSD＜2 %).Separation efficiencies of acetone in the Br-β-CD/OTCEC column decreased 6.77%after 10 days,good stabilization was obtained.
An enzyme is a biological catalyst,which has high efficiency under mild conditions and is highly selective.In the concept of utilizing the natural catalysts to manufacture various products,such as foods or pharmaceuticals,immobilizing enzyme technique was born.The ability to easily separate immobilized enzymes from the reaction mixture offers an additional advantage for multiple use of the enzyme,resulting in reduced cost and time to remove the enzyme from the measurement samples.The preparation of the immobilized microenzyme reactors inside capillaries or channels of the microfluidic devices represents another step in the development of the miniaturized systems for rapid analyses of very small sample sizes.The studied enzymes can be covalently attached on the surface of the capillaries or channels.The advantage of the covalent bonding is that the leakage of the immobilized enzyme is minimized. PAMAM dendrimers stationary phase for OTCEC was prepared successfully using microwave-assisted method.Through the high branches of the dendrimers,the capacity of the surface introduction can greatly increase the immobilized enzyme and reduced the enzyme molecules immobilized in the rigid matrix surface,thus reducing the possibility of changes in conformation to maintain the enzyme activity.In this work,the enzyme micro-reactor was prepared in PAMAM dendrimers modified capillary using glutaraldehyde as a cross-linking agent and aminoacylase as immobilized enzyme.The product of enzyme capillary micro-reactor was separated by capillary zone electrophoresis. Peak area of the product represented the enzyme activity. N-acetyl-DL-methioine was passed through a 15cm enzyme capillary micro-reactor.The results showed that the optimal conditions were, substrate concentration 17.5μg mL-1 having a flow rate of 4μL min-1 at 37℃using phosphate buffer of pH 7.5.Under the optimized conditions, the substrate N-acetyl-DL-methionine showed enzymatic degradation for 10 days consecutive(analyzed 5 times a day).The enzyme capillary micro-reactor showed good stability.
Layered double hydroxides(LDHs) are layered compounds with anion-exchange ability.The intercalation of sulfatedβ-cyclodextrin into LDHs by the ion-exchange method was used.In this study,the sulfatedβ-cyclodextrin intercalated in layered double hydroxides (Mg2Al/SO3-β-CD/LDH) was chosen as stationary phase for open tubular capillary electrochromatography and was applied to separate the receme of 1-phenyl-1,2-ethanediol under the optimized conditions.
采用微波辅助合成方法快速地制备了PAMAM树枝状大分子为固定相的开管毛细管电色谱柱。在微波加热的条件下,通过丙烯酸甲酯与KH 550的端氨基发生Micheal加成反应,得到半代的PAMAM,之后端酯基与乙二胺发生酰胺化反应,得到一代的枝形大分子PAMAM,重复以上,制得二代、三代树枝状大分子修饰的毛细管开管电色谱柱。首次尝试用FITC荧光标记的方法对制得的PAMAM修饰的开管毛细管电色谱柱进行表征,结果表明微波辅助合成方法用于制备PAMAM树枝状大分子修饰毛细管柱是可行的。与常规方法相比,微波辅助合成方法大大地缩短了PAMAM/OTCEC柱的制备周期。获得的3G PAMAM/OTCEC柱对丙氨酸和辅氨酸达到了基线分离。用丙酮考察获得PAMAM/OTCEC柱,迁移时间的RSD＜3.O%,说明制备的毛细管柱具有良好的重现性。用丙氨酸和辅氨酸对3 GPAMAM/OTCEC柱的稳定性进行测试,10天后峰面积RSD＜6.0%,说明制得的开管毛细管色谱柱的具有良好的稳定性。
利用微波辅助合成方法制备β-环糊精衍生物(Br-β-CD)为固定相的开管毛细管电色谱柱。通过FITC荧光标记的方法优化KH 550修饰毛细管柱的微波反应时间,在微波功率140 W,微波温度40℃的条件下,微波反应10 min,KH 550修饰毛细管柱的荧光强度最大。继续通入Br-β-CD,微波反应30 min,制得Br-β-CD/OTCEC柱。获得的毛细管柱对N-乙酰-DL-蛋氨酸、l-苯基-1,2-乙二醇达到基线分离。用丙酮和1-苯基-1,2-乙二醇考察制得毛细管柱的性能,实验结果表明,制得的Br-β-CD/OTCEC柱具有良好的稳定性和重现性。
毛细管酶微反应器是从固定化酶术发展而来的,将酶固定在毛细管内,可以进一步减少酶的消耗量。本文以氨基酰化酶为模型,成功地制备3G PAMAM树枝分子修饰的毛细管柱酶微反应器。通过高分支PAMAM树枝状大分子的引入,可以大大增加表面固定酶的容量,并且减小固定化的酶分子在刚性基体表面出现构像变化的可能性,保持酶的活性。利用毛细管区带电泳(CZE)优化制备的酶微反应器的酶解条件,实验结果表明,酶解温度37℃,17.5μg mL~(-1)N-乙酰-DL-蛋氨酸磷酸缓冲溶液(pH7.5,0.02 moL L~(-1)),底物的流速4μL min~(-1),制备的毛细管酶微反应器具有良好的酶解效果。在优化条件下,考察10天内(每天酶解5次)酶解产物峰面积的变化。实验结果表明,10天后酶解产物的峰面积的RSD<10%,说明制备的毛细管柱酶微反应器具有良好的稳定性。
β-环糊精柱撑水滑石(Mg_2Al/SO_3-β-CD/LD)是一种新型有机/无机杂化的纳米材料。本文以Mg_2Al/SO_3-β-CD/LDH为固定相,制备水滑石内壁修饰的毛细管电色谱开管柱,获得的毛细管柱在pH8.0Tris-HCl缓冲溶液中对1-苯基-1,2-乙二醇达到基线分离(Rs=3.03)。
Open tubular capillary electrochromatography(OTCEC) is one of the most important approaches in capillary electrochromatography(CEC). Microwave irradiation method has become increasingly popular for modern chemical synthesis strategies sine.it's first application towards organic chemistry.Compared with conventional synthesis process, microwave irradiation method has the advantages such as,heating system with uniform temperature,selective heating and no hysteresis effect. Polyamidoamine(PAMAM) dendrimers stationary phase for OTCEC was prepared from silanized fused-silica capillaries by 3-Aminopropylmethyldimethoxysilane(KH 550) with repeating tertiary amine/amide branching units using microwave-assisted method. Fluorescein isothiocyanate(FITC) is one of the most commonly used fluorescent dyes which act as an amine reactive label in bioassay.FITC was used as a label for the characterization of PAMAM dendrimer modified capillary column.The results verified that the preparation of PAMAM dendrimer modified column with microwave irradiation is feasible.Comparing with the conventional synthesis,microwave irradiation significantly improved the reaction speed and shortened the preparation time.The separation of Alanine and Proline was demonstrated with prepared PAMAM/OTCEC column.The results showed that the separation was gradually improved with the increasing of PAMAM generation,and the baseline separation can be obtained by G3 PAMAM dendrimer modified capillary column.The reproducibility of the PAMAM/OTCEC column was evaluated by measuring the RSD of the migration time.The results showed that the reproducibility of the column was satisfactory(RSD＜3.0%).The rate of column efficiency decline was used to examine the stability of G3 PAMAM/OTCEC column. Alanine and Proline was used to determine the stability for consecutive 10 days,the results showed that the columns have a good stability.
At present,cyclodextrins(CDs) and their derivatives are the most widely applied chiral selector due to its low price,low UV absorbance, and unique structure.In this study,FITC was used as label for the characterization of KH 550 modified capillary column prepared by microwave assisted method.The results verified that the preparation of KH 550 modified column with microwave irradiation is feasible. Bromoacetate-substituted-β-cyclodextrins(Br-β-CD) have been successfully bonded to KH 550 modified column as chiral stationary phase in OTCEC.Comparing with the conventional synthesis,microwave assisted process significantly improved the reaction speed and shortened the preparation time from 16h to 40min.Baseline chiral separation of N-acetyl-DL-methionine and 1-phenyl-1,2-ethanediol was obtained in Br-β-CD modified column.The reproducibility of the Br-β-CD/OTCEC column was evaluated by measuring the RSD of the migration times of chiral compound 1-phenyl-1,2-ethanediol.The migration time data indicated that the reproducibility of the column was satisfactory(RSD＜2 %).Separation efficiencies of acetone in the Br-β-CD/OTCEC column decreased 6.77%after 10 days,good stabilization was obtained.
An enzyme is a biological catalyst,which has high efficiency under mild conditions and is highly selective.In the concept of utilizing the natural catalysts to manufacture various products,such as foods or pharmaceuticals,immobilizing enzyme technique was born.The ability to easily separate immobilized enzymes from the reaction mixture offers an additional advantage for multiple use of the enzyme,resulting in reduced cost and time to remove the enzyme from the measurement samples.The preparation of the immobilized microenzyme reactors inside capillaries or channels of the microfluidic devices represents another step in the development of the miniaturized systems for rapid analyses of very small sample sizes.The studied enzymes can be covalently attached on the surface of the capillaries or channels.The advantage of the covalent bonding is that the leakage of the immobilized enzyme is minimized. PAMAM dendrimers stationary phase for OTCEC was prepared successfully using microwave-assisted method.Through the high branches of the dendrimers,the capacity of the surface introduction can greatly increase the immobilized enzyme and reduced the enzyme molecules immobilized in the rigid matrix surface,thus reducing the possibility of changes in conformation to maintain the enzyme activity.In this work,the enzyme micro-reactor was prepared in PAMAM dendrimers modified capillary using glutaraldehyde as a cross-linking agent and aminoacylase as immobilized enzyme.The product of enzyme capillary micro-reactor was separated by capillary zone electrophoresis. Peak area of the product represented the enzyme activity. N-acetyl-DL-methioine was passed through a 15cm enzyme capillary micro-reactor.The results showed that the optimal conditions were, substrate concentration 17.5μg mL-1 having a flow rate of 4μL min-1 at 37℃using phosphate buffer of pH 7.5.Under the optimized conditions, the substrate N-acetyl-DL-methionine showed enzymatic degradation for 10 days consecutive(analyzed 5 times a day).The enzyme capillary micro-reactor showed good stability.
Layered double hydroxides(LDHs) are layered compounds with anion-exchange ability.The intercalation of sulfatedβ-cyclodextrin into LDHs by the ion-exchange method was used.In this study,the sulfatedβ-cyclodextrin intercalated in layered double hydroxides (Mg2Al/SO3-β-CD/LDH) was chosen as stationary phase for open tubular capillary electrochromatography and was applied to separate the receme of 1-phenyl-1,2-ethanediol under the optimized conditions.
引文
[1]Otsuka S,Listowsky L.High-resolution preparative electrochromatography for purification of two subunit types of ferritin[J].AnaL.Bioehem.,1980,102:419-422
[2]Tsuda T,Nomura K,Nakagawa G.Separation of organic and metal ions by high-voltage capillary electrophoresis[J].J Chromatogr.,1983,264:385-392
[3]Jorgenson J W,Lukacs K D.Zone electrophoresis in open-tubular glass capillaries[J].Anal.Chem.,1981,53:1298-1302
[4]Tsuda T.Electrochromatography using high applied voltage[J].Anal.Chem.,1987,59:521-523
[5]Mould D L,Synge R L M.Electrokinetic ultrafiltration analysis of polysaccharides a new approach to the chromatography of large molecule.Analyst,1952,77:964-968
[6]Pretorius V,Hopkins B J,Schieke J D.Electrouosmosis:a new concept for high-speed liquid chromatography[J].J.Chromatogr.,1974,99:23-30
[7]Otsuka S,Listowsky I.High-resolution preparative electrochromatography for purification of two subunit types of ferritin.Anal.Biochem.1980,102:419-422.
[8]Jorgenson J W,Lukaes K D.High-resolution separations based on electrophoresis and electroosmosis[J].J.Chromatogr.1981,218:209-216
[9]Tsuda T,Nomura K,Nakagawa G J.Open-tubular microcapillary liquid chromatography with electro-osmosis flow using a UV detector[J].J.Chromatogr.,1982,248:241-247
[10]Knox J H.Miniaturization in pressure and electroendosmotically driven liquid chromatography:some theoretical considerations[J].Chromatographia,1984,24,135-143
[11]Knox J H.Thermal effects and band spreading in capillary electro-separation[J].Chromatographia,1988,26,329-337
[12]Lord G A,Gordon D B,Tetier L W,et.al.Electrochromatography-electrospray mass spectrometry of textile dyes[J].J Chromatogr A.,1995,700:27-33
[13]Fujimoto C.Charged polyacrylamide gels for capillary electrochromatographic separations of uncharged,low molecular weight compounds[J].Anal.Chem.,1995,67(13):2050-2053.
[14]Mohammad J,Zeerak A,Hjerten S.Dye-ligand affinity-chromatographyon continuous beds[J].Biomed.Chromatogr.1995,9:80-84.
[15]Yan C,Dadoo R,Zare R N,Gradient elution in capillary electrochro-matography[J].Anal.Chem.,1996,68,:2726-2730
[16]Choudhary G.,Horvath C,Dynamics of capillary electrochromatography:experimental study on the electroosmotic flow and conductance in open and packed capillaries[J].J.Chromatogr.A.,1997,781:161-183
[17]Pusecher K,Schewitz J,Bayer E.On-line coupling of capillary electr-ochromatography,capillary electrophoresis and capillary HPLC with nuclearmagnetic resonance spectroscopy [J].Anal.Chem.,1998,70:3280-3285
[18]Wen E,Asiaie R,Horwath C.Dynamics of capillary electrochromatography Ⅱ.Comparison of column efficiency parameters in microscale high-performance liquid chromatography and capillary electrochromatography[J].J.Chromatogr.A.,1999,11:263-296
[19]Stol R,Kok-Wim Th,Poppe H.Capillary electrochromatography with macroporous particles [J].J.Chroamtogr.A.,1999,953:45-54
[20]Zhang L,Zhang Y,Zou H F.Properties and applications of mixed packing capillary electrochromatography[J].J.High Resol.Chromatogr.,1999,22:666-670
[21]Ye M,Zou H F,Zhang Y.Capillary electrochromatography with a silica column with a dynamically modified cationic sufactant[J].J.Chromatogr.A,1999,855:137-145
[22]Wanders B J,Van D,Goor A M,Everaerts F M,Methods for on-line determination and control of electroendosmosis in capillary electrochromatography andelectrophoresis[J].J.Chromatogr.,1989,490:89-93
[23]陈义.毛细管电泳技术及应用[M].北京:化学工业出版社,2000.1-78
[24]邓延卓,何金兰.高效毛细管电泳[M].北京,科学出版社,2000年,p24
[25]Ye M L,Zou H F,Ni J Y.Separation of acidic compounds by strong anion-exchange capillary electrochromatography[J].J.Chromatogr.A.,2000,887:223-231
[26]王俊德,商振华,郁蕴璐,高效液相色谱法[M]北京,中国石化出版社,1992年,p12
[27]邹汉法,刘震,叶明亮,张玉奎,毛细管电色谱及其应用[M].北京,科学出版社,2001年,p75
[28]Golay M J E.Vapor phase chromatography and telegrapher's equation[J].Anal.Chem.,1957,29:928-932
[29]Ishii D,Hibi K,Asai K.etal.Studies of microhigh-performance liquid chromatography:Ⅱ.Application to gel permeation chromatography of techniques developed for micro high-perfromance liquid chromatography[J].J.Chromatogr.,1978,151:147-154
[30]尤慧艳,张维冰,张玉奎,等.毛细管电色谱柱制备技术的进展[J].色谱,2005,23(5):464-469
[31]Li S,Lloyd D K.Direct chiral separation by capillary electrophoresis using capillaries packed with an al-acid glycoprotein chiral stationary phase[J].Anal.Chem.,1993,65:3684-3691
[32]Knox J H.Terminology and nomenclature in capillary electroseparation systems[J].J.Chromatpgr.,1994,680:3-13
[33]Tsuda T,Nomura K,Nakagawa G J.Open-tubular microcapillary liquid chromatography with electro-osmosis flow using a UV detector[J]J.Chromatogr.,1982,248:241-247
[34]Martin M,Guioehon G.Axial dispersion in open-tubular capillary liquid chromatography with electroosmotic flow[J].AnaLChem.,1984,56(4):614-620
[35]Martin M,Guioehon G.,Walboel Y,et al.Broadening in open-tubular liquid chromatography with electroosmotic flow[J].Anal.Chem.1985,57(2):559-561
[36]Bruin G.J M,Toek P P H,Krak J C,PoPPe H.Electrically driven open-tubular liquid chromatography[J].J Chromatogr.,1990,517:557-572
[37]Guo Y,Colon L A.A stationary phase for open tubular liquid chromatography and electrochromatography using sol-gel technology[J].Anal.Chem.,1995,67(15):2511-2516
[38]Pfeffer W D,Yeung E S.Open-tubular liquid chromatography with surfactant-enhanced electroosmotic flow[J].Anal.Chem.,1990,62(20):2178-2182
[39]Pfeffer W D.Yeung E S.Electroosmotically driven electrochromatography of anions having similar electrophoretic mobilities by ion pairing[J].J.Chromatogr.A,1991,557:125-136
[40]Mayer S,SchurigV.Enantiomer separation by electrochromatography on capillaries coated with chirasil-dex[J].J.High Resolut Chromatogr&Chromatogr Commun,1992,15:129-131
[41]Mayer S,Schurig V.Enantiomer separation by electrochromatography in open tubular columns coated with chirasil-dex[J].J.Liquid.Chromatogr.,1993,16:915-931
[42]Arnlstrong D W,Tang Y,Ward T,Niehols M.Derivatized cyclodextrins immobilized on fused-silica capillaries for enantiomeric separations via capillary electrophoresis,gas hromatography,or supercritical fluid chromatography[J].Anal.Chem.,1993,65(8):1114-1117
[43]Sehurig V,Jung M,Mayer S,et al.Toward unified enantioselective chromatography with a single capillary column coated with chirasil-dex[J].Angew.Chem.I nt.ED.Eng.,1994,33:2222.-2232
[44]Sehurig V,Jung M,Mayer S,et al.Unified enantioselective capillary chromatography on a Chirasil-DEX stationary phase Advantages of column miniaturization[J].J Chromatogr A,1995,694:119-128
[45]Francotte E,Jung M.Enantiomer separation by open tubular liquidChromatography and electrochromatography in cellulose2coated capillaries[J].Chromatographia,1996,42:521-527
[46]Cikalo M G.,Bartle K D,Myers P.Behavior of Cation-Exchange Materials in Capillary Electrochromatography[J].Anal.Chem.,1999,71(9):1820-1825
[47]Liu Z,Zou H F,Ni J Y,et al.Open tubular capillary electrochromatography with adsorbed stationary phase[J].Anal.Chim.Acta,1999,378:73-76
[48]Liu Z,Zou H F,Ye M L,el at.Study of physically adsorbed stationary phases for open tubular capillary electrochromatography[J].Electrohoresis,1999,20:2891-2897
[49]Liu Z,Otsuka K,Terabe S.Chiral separation by open tubular capillary electrochromatography with adsorbed avidin as a stationary phase[J].J.Sep.Sci.,2001,24(1):17-26
[50]Breadmore M C,Maeka M,Avdalovie N,et al.Open-tubular ion-exchange capillary electrochromatography of inorganic anions[J].Analyst.2000,125:1235
[51]Hibi K,Tsuda T.Studies of open-tubular microcapillary liquid chromatography:Ⅲ.β,β＇-oxydipropionitrile and ethylene glycol stationary phases[J].J.Chromatogr.,1979,175:105-111
[52]Ishii D,Tsuda T,Takeuchi T.Studies of open-tubular micro-capillary liquid chromatography:IV.Soda-lime glass columns treated with alkaline solution[J].J.Chromalogr.,1979,185:73-78
[53]Tsuda T,Tsuboi K,Nakagama G,Open-tubular microcapillary liquid chromatography with 20-μtm I.D.columns[J].J.Chromatogor.,1981,214:283-290
[54]Pesek J J,Matyska M T,Separation of tetracyclines by high-performance capillary electrophoresis and capillary electrochromatography[J].J.Chromatogr.A,1996,736:313-320
[55]Pesek J J,Matyska M T,Mauskar L.Separation of proteins and peptides by capillary electrochromatography in diol-and octadecyl-modified etched capillaries[J].J Chromatogr A,1997,763:307-314
[56]Pesek J J,Matyska M T,Cho S J.Open tubular capillary electrochromatography in etched,chemically modified 20 μtm I.D.capillaries[J].J.Chrolnalogr.A,1999,845:237-246
[57]Matyska M T,Pesek J.J,Katrekar A.Open tubular capillary electrochromatography using etched fused-tilica tubing modified with chemically bonded liquid crystals[J].Anal.Chem.,1999,71:5508-5514
[58]Matyska M T,Pesek J J,Boysen R I.Electrochromatographic characterization of etched chemically-modified capillaries with small synthetic peptides[J].J.Chromatogr A,.2001,924:211-221
[59] Catabay A P, Sawada H, Jinno K, et al. Determination of cefobid using adsorptive stripping voltammetric technique in different media [J]. Electrophoresis, 1998, 5 (1): 85-89
[60] Guan N, Zeng Z R, Wang Y C. Open tubular capillary electrochromatography in fused-silica capillaries chemically bonded with macrocyclic dioxopolyamine [J]. Anal. Chim. Acta, 2000, 418: 145-151.
[61] Toek P P H, Boshoven C, Poppe H. Performance of porous silica layers in open-tubular columns for liquid chromatography [J]. J. Chromatogr. A, 1989,477: 95-106
[62] Bruin G J M, Toe K P H, Kraak J C. Electrically driven open-tubular liquid chromatography [J]. J. Chromatogr. A, 1990, 517: 557-572
[63] Crego A L, Diezmasa J C, Dabrio M V. Preparation of open tubular columns for reversed-phase liquid chromatography[J]. Anal. Chem., 1993, 65: 1613-1624
[64] Narang P,Col6n L A.Sol-gel derived fluorinated stationary phase for open tubular capillary electrochromatography[J], J. Chromatogr. A, 1997,773: 65-72
[65] Freitag R,Constantin s. Investigation of factors influencing the performance of open-tubular stationary phases in capillary electrochromatography [J]. J. Sep. Sci.2003, 26: 835-843
[66] Constantin S,Freitag R,Solignac D,Sayah A,Gijs M A M.Utilization of the sol-gel technique for the development of novel stationary phases for capillary electrochromatography on a chip[J]. Sen. Actuat. B 2001,78: 267-272
[67] Rodriguez S A, colon L A. Investigations of a sol-gel derived stationary phase for open tubular capillary electrochromatography [J]. Anal. Chim. Acta, 1999, 397: 207-215
[68] Constantin S, Freitag R. Preparation of stationary phases for open-tubular capillary electrochromatography using the sol-gel method [J] J. Chromatogr. A, 2000, 887: 253-263
[69] Hayes J D, Malik A. Sol-gel open tubular ODS columns with reversed electroosmotic flow for capillary electrochromatography [J]. Anal. Chem., 2001,73: 987-996
[70] Huang X, Zhang J, Horva'th C. Capillary electrochromatography of proteins and peptides with porous layer open-tubular columns [J]. J. Chromatogr. A, 1999, 858: 91-101
[71] Xu W S, Regnier F E. Electrokinetically-driven cation-exchange chromatography of proteins and its comparison with pressure-driven high-performance liquid chromatography [J]. J. Chromatogr. A, 1999, 853: 243-256
[72] Liu Z, Zou H F, Ni J Y. Open tubular capillary electrochromatography with adsorbed stationary phase[J]. Anal. Chim. Acta., 1999, 378: 73-76
[73] Charvatova J, KasickaV, Kral V, et al. Capillary electrochromatographic study of the interactions of porphyrin derivatives with amino acids and oligopeptides [J]. J. Chromatogr. B, 2002, 770: 165-175
[74] Charvatova J, KasickaV, Deyl Z, et al. Open tubular capillary electrochromatography of uuderivatized amino acids using Rh (III) tetrakis (phenoxyphenyl) porphyrinate as wall modifier [J]. J. Chromatogr. A., 2003, 990: 159-167
[75] Shiue C C, Lin S Y, Liu C Y. Chemically bonded fulleredn C_(60) capillary column for the electrophoretic separation of plant phenols [J]. Chin. J. Chem. Soc, 2001,48: 1029-1034
[76] Zeng Z R, Xie C H, Li H B, et al. Open-tubular capillary electrochromatography using capillary columns chemically bonded with the new host molecules calix [6] crown, calix [6] arene [J]. Electrophoresis, 2002, 23: 1272-1278
[77] Wu X J, Liu H X., Liu H, et al. Preparation and characterization of ptertbutylcalix [8] arene bonded capillaries for open-tubular capillary electrochromatography [J]. Anal. Chim. Acta., 2003,478:191-197
[78]Mayer S,Schurig V.Enantiomer separation by eletrochromatography in open tubular columns coated with chirasil-dex[J].J.Liq.Chromatogr.,1993,16(4):915-931
[79]Li S,Lloyd D K.Direct chiral separation by capillary electrophoresis using capillaries packed with an al-acid glycoprotein chiral stationary phase[J].Anal.Chem.,1993,65:3684-3691
[80]Lloyd D K,Li S,Ryan P.Protein chiral selectors in free-solution capillary electrophoresis and packed-capillary electrochromatography[J].J.Chromatogr.A,1995,694:285-296
[81]Zhao Y X,Zhao R,Shangguan D H,e tal.A new type of capillary column for open-tubular electrochromatography[J].Electrophoresis,2002,23(17):2990-2995
[82]Pfeffer W D,Yeung E S.Electroosmotically driven electrochromatography of anions having similar electrophoretic mobilities by ion pairing[J].J.Chromatogr.A,1991,557:125-136
[83]Schweitz L,Andersson L I,Nilsson S.Capillary electrochromatography with predetermined selectivity obtained through molecular imprinting[J].Anal.Chem.,1997,69(6):1179-1183
[84]Wu J T,Huang P,Li M X,et al.Open-tubular capillary electrochromatography with an on-line ion trap storage/reflectron time-of-flight mass detector for ultrafast peptide mixture analysis[J].Anal.Chem.,1997,69(3):320-326
[85]曾昭睿,谢传辉,王园朝,等.溶胶-凝胶法制备丙二酰胺型二氧大环多胺用作开管柱电色谱固定相的研究[J].高等学校化学学报,2001,22(7):1108-1110
[86]Kapnissi C P,Valle B C,Warner I M.,et al.Chiral separations using polymeric surfactants and polyelectrolyte multilayers in open-tubular capillary electrochromatography[J].Anal.Chem.,2003,75(22):6097-6104
[87]Bhleier E,Wehner W,Vogtle F."Casad" and "Nonskid-chain-like" Syntheses of Moleeular Cavity Topologies[J].Synthesis,1978,2:155-158
[88]Deenkewalter R G,Kole J,LukasavgaeW J.Moeormoleeular highly barnehed homogeneous compound based on lysine units[P].US:4289 872,1981-07
[89]Tomalia D A,Bkaer H,Deward J,et al.A new classof polymesr:sartbusrt-dendritie macromoleeules[J].Polym.J.1985,17:117-132
[90]Newkome G R,Yao Z Q,Bkaer G R,et al.Casedae moleeules:A new approach to mieelles a arboorl[J].J.Ogr.Chem.,1985,50:2003-2004
[91]Hawker C J,Freehet J M J.Preparation of polymers with contorlled moleeular aerhiteetuer.A new convergent approaeh to dendritic macromoleeules[J].J.Am.Chem.Soc.,1990,112:7638-7647
[92]Serroni S,Denci G,Gapgaha S.Arborist form luminescent and redox-actjve transition metal complex fragments[J].Angew.Chem.hnt.Ed.Eng.,1992,31(11):1493-149
[93]Vogtle F,Gestermann S,Hesse R,et al.Functional dendrimers[J].J.Prog.Polym.Sci.,2000,25:987-1041
[94]Lothian-Tomalia M K,Hedstrand D M,Tomalia D A.The divergent synthesis of poly(thioether) dendrimers.amplified,genealogically directed synthesis leading to the de gennes dense packed state[J].Tetrahedron,1997,53:15495-15513
[95]Furka A,Sebastyen F,Asgedom M,et al.General method for rapid synthesis of multicomponent peptide mixtures[J].J.Pept.Protein.Res.,1991,37:487-493
[96]Haag R.Dendrimers and hyperbranched polymers as high-loading supports for organic synthesis[J].Chem.Eur.J.,2001,7:327-335
[97]Lebreton S,Monaghan S,Bradley M.Solid-phase dendrimer chemistry:synthesis and application[J].J.Aldrichimica.Acta.,2001,34(3):75-83
[98]Genderen M H P,Baars M W P L,Hest J C M,et al.Obser vingin dividual generationsin poly(propyleneimine) dendrimers withnatural-abundancel(15)~N-NMR spectroscopy[J].J.Reel.Trav.Chim.Pays-Bas.,1994,113:573-574
[99]吴文娟,徐冬梅,张可达,等.聚酰胺-胺树状大分子的应用[J].高分子学报,2003,8:67-71
[100]赵立文,范青华,周海峰,等.树状大分子在有机合成中的应用[J].化学进展,2006,18(2):317-330
[101]Valerio C,Alonso E,Ruiz J,et al.A polycationic metallodendrimer with 24 organoiron termini which senses chlorideand bromide anions[J].Angew.Chem.Int.Ed.Engl,1999,38:1747-175
[102]Tanaka N,Fukutome T,Tanigawa T,et al.Structural selectivity provided by starburst dendrimers as pseudostationary phase in electrokinetie chromatography[J].J.Chromatogr A,1995,699:331-341
[103]Shi X,Lesniak W,Islam MT,et al.Comprehensive characterization of surfaee-functionalized poly(amidoamine) dendrimers with acetamide,hydroxyl,and carboxyl groups[J].Colloid.Surface.A:Physicochem.Eng.Aspects,2006,272:139-150
[104]Shi X,Banyai I,Rodriguez K,etal.Electrophoretic mobility and molecular distribution studies of poly(amidoamine) dendrimers of defined charges[J].Electrophoresis,2006,27:1758-1767
[105]Chao H C,Hanson J E.Dendritic polymers as bonded stationary phase's incapillary electrochromatography[J].J.Sep.Sci.,2002,25:345-350
[106]张志胜,杨屹.以树形大分子聚酰胺-胺(PAMAM)为键合固定相的开管毛细管电色谱柱的制备及评价[J].高等学校化学学报,2006,27(1):47-51
[107]郭宝晶,杨屹,苏萍.牛血清白蛋白-PAMAM修饰的开管毛细管电色谱柱的制备[J].高等学校化学学报,2007,28(7):1267-1269
[108]王静,姜凤超.微波有机合成反应的新进展[J].有机化学,2002,22(3):212-219
[110]Gedye R,Snuth F,Westaway K,et al.The use of microwave ovens for rapid organic synthesis[J].Tetrahedron.Lett.,1986,27:279-282
[111]Giguere R.J,Bray T L,Duncan S M,et al.Application of commercial microwave ovens to organic synthesis[J].Tetrahedron.Lett.,1986,27:4945-4948
[112]Vanderhoff J W.Carrying out chemical reactions using microware[P].US 3432413.1969-03-11
[113]孟庆华,陈虹锦,方能虎,等.面向化学研究的微波反应器[J].实验室研究与探索,2003,22(3):94-97
[114]Willging E M.Microware digestion techniques in the sequential extraction techniques in the sequential extraction of calcium,iron,chromium,manganese,leasd and zinc in sedimenes [J].Anal.Chem.,1987,59(7):938-945
[115]梅乐和,岑沛霖.现代酶工程.化学工业出版社[M].2002,92-1451
[116]w Aehle,林章凛,李爽.工业酶—制备与应用[M].化学工业出版社,2002,22-85
[117]马俊锋,段继诚,梁振,等.固定化酶反应器及其在蛋白质组研究中的应用[J].分析化学,2006,34(11):1649-1655
[118]肖海军,贺筱蓉.固定化酶及其应用研究进展[J].生物学通报,2001,36:9-10
[119]刘建龙,王瑞明,刘建军,等.酶的固定化技术研究进展[J].中国酿造,2005,9:4-6
[120]Yang J,Hage D S.Chiral separations in capillary electrophoresis using human serum albumin as a buffer additive[J].Anal.Chem.,1994,66:2719-2725
[121]Charvatova J,Deyl Z,Kasicka V,dt al.Open tubular capillary electrochromatography of underivatized amino acids using Rh(Ⅲ) phenoxyphenyl porphyrinate as wall modifier[J].J.Chromatogr.A,2003,990:159-167
[122]Ellerby L M,Nishida CR,Nishida F,et al,Encapsulation of proteins in transparent porous silicate glasses prepared by the sol-gel method[J].Science,1992,255:1113-1115
[123]Edmiston P L,Wambolt C L,Smith M K.Spectroscopic characterization of albumin and myoglobin entrapped in bulk sol-gel glasses[J].Colloid.Interface.Sci.,1994,163:395-406
[124]Braun S,Rappoport S,Zusman R.Biochemically active sol-gel glass:the trapping of enzymes[J].J.Mater.Lett.,1990,10(1-2):1-5
[125]Miller S A,Hong E D and Wright D.Rapid and Efficient enzyme encapsulation in a dendrimer silica nanocompositea[J].Macromol.Biosci.,2006,6:839-845
[126]Flora K.Brennan J D.Fluorometric detection of Ca~(2+) based on anInduced change in the conformation of sol-gel entrapped parvalbumin[J].Anal.Chem.,1998,70:4505-4513
[127]Svobodova L,Snejdarkova M,Hianik T.Properties of glucose biosensors based on dendrimer layers.Effect of enzyme immobilization[J].Anal.Bioanal.Chem.,2002,373:735-741
[128]吴红,姜忠义.溶胶-凝胶法包埋生物活性分子[J].现代化工,2002,22(7):60-65
[129]Minakuchi H,Nakanishi K,Soga N,Ishizuka N,et al.Octadecylsilylated porous silica rods as separation media for reversed-phase liquid chromatography[J].Anal.Chem.,1996,68:3498-3501
[130]Ishizuka N,Minakuchi H,Nakanishi K,etal.Performance of a monolithic silica column in a capillary under pressure-driven and electrodriven conditions[J].Anal.Chem.,2000,72:1275-1280
[131]Birnbaumt S,Nilsson S.Protein-based capllary affinity gel electropgoresis for the separation of optbal Isomers[J].Anal.Chem.,1992,64:2872-2874
[132]Licklider L,Kuhr W.Optimization of on-line peptide mapping by capillary zone electrophoresis[J].Anal.Chem.,1994,66:4400-4407
[133]Licklider L,Kuhr W G,Lacoy M P.On-line microreactors/capillary electrophoresis/Mass spectrometry for the analysis of proteinsand peptides[J].Anal.Chem.,1995,67:4170-4177
[134]Ye M,Hu S,Schoenherr RM.On-line protein digestion and peptide mapping bycapillary electrophoresis with post-column labeling for laser-induced fluorescence detection[J].Electrophoresis,2004,25:1319-1326
[135]Sakai-Kato K,Kato M,Toyo'oka T.On-Line Trypsin-encapsulated enzyme reactor by the sol-gel method integrated into capillary electrophoresis[J].Anal.Chem.,2002,74:2943-2949
[136]Kato M,Sakai-KatoK,Jin H M,etal.Integration of on-line protein digestion,peptide separation,and protein identification usingpepsin-coated photopolymerized sol-gel colunm sand capillary electrophoresis/mass spectrometry[J].Anal.Chem.,2004,76:1896-1902
[137]Amankwa LN,Kuhr WG.Trypsin-modified fused-silica capillary microreactor for peptidemapping by capillary zone electrophoresis[J].Anal.Chem.,1992,64:1610-1613
[138]Krvenkova J,Bilkova Z,Foret F.Characterization of a monolithic immobilized trypsin microreactor with on-line coupling to ESI-MS[J].J.Sep.Sci.,2005,28:1675-1684
[139]Sakai-Kato K,Kato M,Toyo'oka T.On-Line Trypsin-Encapsulated Enzyme Reactor by the Sol-Gel Method Integrated into Capillary Electrophoresis[J].Anal.Chem.,2002,74:2943-2949
[1]Xu L,Sun Y.Fabrication and characterization of open tubular CEC modified with tentacle-type metal-chelating polymer chains[J].Electrophoresis,2007,28:1658-1667
[2]Eeckhaut A V,Michotte Y.Chiral separations by capillary electrophoresis:Recent developments and applications[J].Electrophoresis,2006,27:2880-2895
[3]Jorgenson J W,Lukacs K D.Zone electrophoresis in open-tubular glass capillaries[J].Anal.hem.,1981,53:1298-1302
[4]Terabe S,Otsuka K,Ichikawa K,et al.Electrokinetic separations with micellar solutions and open-tubular capillaries,(Direct analysis of thin-layer chromatography spots by fast atom bombardment mass spectrometry)[J].Anal.Chem.,1984,56:111-113
[5]Jingwu Kang,Dorothee Wistuba,Volker Schurig,Recent progress in enantiomeric separation by capillary electrochromatography[J].Electrophoresis,2002,23:4005-4021
[6]寿崇琦,邢希学,康杰分等.有机硅树枝状大分子涂层毛细管电泳柱的制备及性能评价[J].分析化学,2008,36(2):167-171
[7]Tomalia D A,Berry.V,Hall M,et al.Communications to the Editor[J].Macromolecules,1987,20:1164-1167
[8]Shi X,Majoros I J,Patri A K,et al.Molecular heterogeneity analysis of poly(amidoamine)dendrimer-based mono-and multifunctional nanodevices by capillary electrophoresis[J].Analyst,2006,131:374-381
[9]Shi X,Majoros I J,Patri A K,et al.Electrophoretic mobility and molecular distribution studies of poly(amidoamine) dendrimers of defined charges[J].Electrophoresis,2006,27:1758-1767
[10]Lebreton S,Monaghan S,Bradley M.Solid-phase dendrimer chemistry:synthesis and applications[J].Aldrichimica Acta,2001,34(3):75-82.
[11]Swali V,Wells N J,Langley G J,et al.Solid-phase dendrimer synthesis and the generation of super-high-loading resin beads for combinatorial chemistry[J].J.Org.Chem,1997,62:4902-4903.
[12]张志胜,杨屹.以枝形大分子聚酰胺-胺(PAMAM)为键合固定相的开管毛细管电色谱柱的制备及评价[J].2006,27(1):47-51
[13]郭宝晶,杨屹,苏萍.牛血清白蛋白2PAMAM修饰的开管毛细管电色谱柱的制备[J].2007,28(7):1267-1269
[14]Gedye R,Smith F,Westanay K,et al.The use of microwave ovens for rapid organic synthesis[J].Tetrahedron Lett,1986,27(3):279-282
[15]Giguere R J,Bray T L,Duncan S M.Application of commercial microwave ovens to organic synthesis[J].Tetrahedron Lett.,1986,27(41):4945-4948
[16]金钦汉.微波化学[M].北京:科学技术出版社,2001.126-146
[17]戈明亮.微波在聚合物科学中的应用[J].合成材料老化与应用,2004,33(2):37-40
[18]倪春梅,盛凤军.微波合成技术及在有机合成中的应用[J].广州化工,2004,32(2):11-14
[19]杨莹丽,于东冬,刘岩,等.合成树枝状高分子聚酰胺-胺及其荧光性质[J].辽宁石油化工大学学报,2006,26(4):56-57
[20]Zhang Y,Ye X,Tian M,etal.Novel method to prepare polystyrene-based monolithic columns for chromatographic and electrophoretic separations by microwave irradiation[J].J. Chromatogr.A,2008,1188:43-49
[21]Wang Y C,Zeng Z R,Guan N,el at.Sol-gel technique for the preparation ofβ-cyclodextrin derivative stationary phase in open-tubular capillary electrochromatography[J].Electrophoresis,2001,22:2167-2172
[22]Lin X,Wang J,Li L,et al.Separation and determination of five major opium alkaloids with mixed mode of hydrophilic/cationexchange monolith by pressurized capillary electrochromatography[J].J.Sep.Sci.2007,30:3011-3017
[23]Imhof A,Megens M,Engelberts J J,et al.Spectroscopy of Fluorescein(FITC) dyed colloidal silica spheres[J].J.Phys.Chem.B 1999,103:1408-1415
[24]Son Y J,Jang J,Cho Y W,et al.Biodistribution and anti-tumor efficacy of doxorubicin loaded glycol-chitosan nanoaggregates by EPR effect,J.Control.Release,2003,91:135-145
[25]Hungerford G,Benesch J,Mano J F,et al.Effect of the labelling ratio on the photophysics of fluorescein isothiocyanate(FITC) conjugated to bovine serum albumin[J],Photochem.Photobiol.Sci.,2007,6,152-158
[26]Onga K K,Jenkins A L,Chengc R,et al.Dendrimer enhanced immunosensors for biological detection[J].Anal.Chim.Acta,2001,444:143-148
[27]Domanski D M,Klajnert B,Bryszewska M,Incorporation of fluorescent probes into PAMAM dendrimers[J],Bioelectrochemistry,2004,63:193-197
[28]Kitagawa F,Inoue K,Hasegawa T,etal.Chiral separation of acidic drug components by open tubular electrochromatography using avidin immobilized capillaries[J].J.Chromatogr.A,2006,1130:219-226
[29]黄坚,陈胜福,曾涛,等.酸碱滴定分析在聚酰胺-胺表征中的应用[J].高分子材料科学与工程,2007,23(4):185-191
[1]G(u|¨)ibitz G,Schmid M G.Chiral separation by capillary electromigration techniques[J].J.Chromatogr.A,2008,1204:140-156
[2]Wang Y,Yin X,Shi M,etal.Probing chiral amino acids at sub-picomolar level based on bovine serum albumin enantioselective films coupled with silver-enhanced gold nanoparticles[J].Talanta,2006,69:1240-1245
[3]张红丽,赵亮,明永飞,等.模拟移动床色谱分离制备手性农药甲霜灵的研究[J].分析实验室,2007,26(10):14-18
[4]陈立仁.液相色谱手性分离[M].北京:科学出版社,2006.
[5]Peng A,Li R,Hu J,et al.Flow rate gradient high-speed counter-current chromatography separation of five diterpenoids from Triperygium wilfordii and scale-up[J].J.Chromatogr.A,2008,1200:129-134
[6]Fu X,Huang L,Gao F,etal,Carboxymethyl chitosan-coated capillary and its application in CE of proteins[J].Electrophoresis,2007,28:1958-1963
[7]Wang Z,Ouyanga J,Baeyens W R G,Recent developments of enantioseparation techniques for adrenergic drugs using liquid chromatography and capillary electrophoresis:A review[J].J.Chromatogr.B,2008,862:1-14
[8]Wahl A,Schnell I,Pyell U.Capillary electrochromatography with polymeric continuous beds synthesized via free radical polymerization in aqueous media using derivatized cyclodextrins as solubilizing agents[J].J.Chromatogr.A,2004,1044:211-222
[9]Li S,Lloyd D K.Direct chiral separations by capillary electrophoresis using capillaries packed with an acid glycoprotein stationary phase[J].Anal.Chem.,1993,65:3684-3690
[10]Fujimura K,Ueda T,Ando T.Retention behavior of some aromatic compounds on chemically bonded cyclodextrin silica stationary phase in liquid chromatography[J].Anal.Chem.,1983,55(3),446-450.
[11]Tanaka M,Kawaguchi Y,Shono T,Retention behaviour of disubstituted benzene isomers on acetylated cyclodextrin stationary phases[J].J.Chromatogr.A,1983,267:285-292
[12]Matyska M T,Pesek J J,Boysen I,et al,Characterization and applications of etched chemically modified capillaries for open-tubular capillary electrochromatography[J].Electrophoresis,2001,22:2620-2628
[13]Wang Y,Zeng Z,Guan N,et al.Sol-gel technique for the preparation of β-cyclodextrin derivative stationary phase in open-tubular capillary electrochromatography[J].Electrophoresis,2001,22:2167-2172
[14]Wang Y,Xiao Y,Tan Y,et al.Click chemistry for facile immobilization of cyclodextrin derivatives onto silica as chiral stationary phases[J].Tetrahedron Lett,2008,49:5190-5191
[15]杨莹丽,于东冬,刘岩,等.合成树枝状高分子聚酰胺-胺及其荧光性质[J].辽宁石油化工大学学报,2006,26(4):56-57
[16]Giguere R J,Bray T L,Duncan S M.Application of commercial microwave ovens to organic synthesis[J].Tetrahedron Lett.,1986,27(41):4945-4948
[17]金钦汉.微波化学[M].北京:科学技术出版社,2001.126-146
[18]Zhang Y,Ye X,Tian M,etal.Novel method to prepare polystyrene-based monolithic columns for chromatographic and electrophoretic separations by microwave irradiation[J].J.Chromatogr.A,2008,1188:43-49
[19]Gong Y,Lee H K,Application of Cyclam-capped β-cyclodextrin-bonded silica particles as a chiral Stationary phase in capillary electrochromatography for enantiomeric separations[J].Anal.Chem.2003,75:1348-1354
[20]Lee M,Sun C M.Traceless synthesis of hydantoin by focused microwave irradiation[J].Tetrahedron Lett.,2004,45:437-440
[21]Vargas-Dlaz M E,Chaco'n-Garia L,Velazquez P,etal.Enantioselective synthesis of 1-alkyl-substituted 1-phenyl-1,2-ethanediols using a myrtenal-derived chiral auxiliary[J],Tetrahedron-Asymmetr,2003,14:3225-3232
[1]肖海军,贺莜蓉.固定化酶及其应用研究进展[J].生物学报,2001,36(7):9-10
[2]Tang Z,Wang T,Kang J.Immobilized capillary enzyme reactor based on layer-by-layer assembling acetylcholinesterase for inhibitor screening by CE[J].Electrophoresis,2007,28:2981-2897
[3]Krenkova J,Kleparnik K,Foret F.Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries[J].J.Chromatogr.A,2007,1159:110-118
[4]郭忠,张清春,雷政登,等.固定化酶纳升微反应器用于痕量蛋白质快速肽谱分析的研究[J].高等学校化学学报,2002,23(7):1277-1280
[5]马俊锋,段继诚,梁振,等.固定化酶反应器及其在蛋白质组研究中的应用[J].分析化学,2006,34:1649-1665
[6]Sakai-Kato K,Kato M,Toyooka T.On-line trypsin-encapsulated enzyme reactor by the sol-gel method integrated into capillary electrophoresis[J].Anal.Chem.,2002,74(13):2943-2949
[7]Kato M,Sakai-Kato M,Matsumoto N,etal.A protein-encapsulation technique by the sol-gel method for the preparation of monolithic columns for capillary electrochromatography[J].Anal Chem,2002,74:1915-1921
[8]Sakai-Kato K,Kato M,Toyooka T.Creation of an on-chip enzyme reactor by encapsulating trypsin in sol-gel on a plastic microchip[J].Anal Chem,2003,75:388-393
[9]Dulay M T,Baca Q J,Zare R N.Enhanced proteolytic activity of covalently bound enzymes in photopolymerized sol-gel[J].Anal.Chem.2005,77:4604-4610
[10]Bauer M,Griengl H,Steiner W,Kinetic studies on the enzyme(S)-hydroxynitrile lyase from Hevea brasiliensis using initial rate methods and progress curve analysis[J],Biotechnol Bioeng,1999,62:20-35
[11]Ye M L.,Hu S,Schoenherr R M,et al,On-line protein digestion and peptide mapping by capillary electrophoresis with post-column labeling for laser-induced fluorescence detection[J].Electrophoresis,2004,25:1319-1326
[12]Krenkova.J,Foret F.Imniobilized microfluidic enzymatic reactors[J].Electrophoresis,2004,25:3550-3563
[13]Amankwa L N,Kuhr W G,Trypsin-modified fused-silica capillary microreactor for peptidemapping by capillary zone electrophoresis[J].Anal Chem,1992,64:1610-1613
[14]Tang Z M,Kang J W.Enzyme inhibitor screening by capillary electrophoresis with an on-column immobilized enzyme microreactor created by an ionic binding technique[J].Anal Chem.,2006,78:2514-2520
[15]Nashaben W,Rassi Z E.Enzymophoresis of nucleic acids by tandem capillary enzyme reactor-capillary zone electrophoresis[J].J Chromatogr.,1992,596:251-264
[16]Khandare J J,Jayant S,Singh A,et al.Dendrimer versus linear conjugate:influence of polymeric architecture on the delivery and anticancer effect of paclitaxel[J].Bioconjugate chem.,2006,17:1464-1472
[17]Miller S A,Hong E D,Wright D.Rapid and efficient enzyme encapsulation in a dendrimer silica nanocomposite macromolecular bioscience[J].Macromol.Biosci.,2006,6:839-845
[18]Yoon H C,Kim H S.Multilayered assembly of dendrimers with enzymes on gold: thickness-controlled biosensing interface[J].Anal.Chem.,2000,72:922-926
[19]Zhu N N,Gu Y F,Chang Z,et al.PAMAM dendrimers-based DNA biosensors for electrochemical detection of DNA hybridization[J].Electroanalysis,2006,18(21):2107-2114
[20]邓彩,龚跃法.新型树枝状分子复合载体的合成及其对脂肪酶的固定化[J].应用化学,2004,21(10):1024-1028
[1]Reichlew T,Kang S Y,Everhardt D S.The nature of the thermal decomposition of a catalytically active an-ionic claymineral[J].J.Catal.,1986,101(2):352-359
[2]Vaccari A.Clays and catalysis:a promising future[J].Appl.Clay.Sci.,1999,14(4):161-198
[3]Cavani F,Trifiro F,Vaccari C.Hydrotalcite-type anionic clays:Preparation,properties and app lications[J].Catal.toda.,1991,11(2):173-301
[4]孟锦宏,张慧,王治强,等.插层组装超分子结构有机物柱撑阴离子层状材料[J].化学通报,2003.35(2):85-94
[5]Legrouri A,LakraimiM,Barroug A,et al.Removal of the herbicide 2,4-dichlorophenoxyacetate from water to zinc-aluminium-chloride layered double hydroxides [J].Wat.Res.,2005,39(15):3441-3448
[6]Bert F S,Dirk E D V,Mieke B,et al.Transition metal anion exchanged layered double hydroxides as a bioinspired model of vanadium bromop eroxidase[J].J.Catal.,2003,216(122):288-297.
[7]杨巧珍,李峰,段雪.层状双羟基氢氧化物(LDH)的表面有机化研究[J].塑料,2003(3):6-9
[8]景晓燕,郑崇辉,王慧颖,等.水滑石类化合物的研究现状及进展[J].应用科技,2004(10):42-46
[9]吴旭,谢鲜梅,杜亚丽,等.水滑石类材料碱性催化性能的应用研究[J].应用化工,2005(8):469-472
[10]Wang J,Wei M,Rao G,Evans D G,et al.Structure and thermal decomposition of sulfated β-cyclodextrin intercalated in a layered double hydroxide[J].J.Solid.State.Chem.,2004,177:366-371
[11]Zhao Y,Li F,Zhang R,et al.Preparation of layered double-hydroxide nonmaterial with a uniform crystallite size using a new method involving separate nucleation and aging steps[J].J.Solid.State.Chem.,1999,146:506-515
[12]Wei M,Wang J,He J,et al.In situ FI-IR,in situ HT-XRD and TPDE study of thermal decomposition of sulfated-β-cyclodextrin intercalated in layered double by droxides[J].Micropor.Mesopor.Mat.,2005:53-61
[13]Hasegawa J,Masahiro S,Satoru T,et al.High-yield production of optically active 1,2-dios from the corresponding race mates by microbial stereo inversion[J].Agric.Biol.Chem.,1990,54(7):1819-1827.
[14]欧阳健,徐岩,穆晓清.加强辅酶循环再生实现不对称合成(S)-1-苯基-1,2-乙二醇[J].四川大学学报(自然科学版),2007,44(4):561-866
[15]Hyungdon Y,Yung H Y,Byung K C,et al.Simultaneous synthesis of enantiomerically pure (R)-1,2-phenylethanol and(R)-α-methylbenzylamine from racemic α-methylbenzylamine using ω-transaminase/ alcohol dehydrogenase/ glucose dehydrognease coupling reaction [J].Biotechnol.Letters,2003,25:809-812
[2]Tsuda T,Nomura K,Nakagawa G.Separation of organic and metal ions by high-voltage capillary electrophoresis[J].J Chromatogr.,1983,264:385-392
[3]Jorgenson J W,Lukacs K D.Zone electrophoresis in open-tubular glass capillaries[J].Anal.Chem.,1981,53:1298-1302
[4]Tsuda T.Electrochromatography using high applied voltage[J].Anal.Chem.,1987,59:521-523
[5]Mould D L,Synge R L M.Electrokinetic ultrafiltration analysis of polysaccharides a new approach to the chromatography of large molecule.Analyst,1952,77:964-968
[6]Pretorius V,Hopkins B J,Schieke J D.Electrouosmosis:a new concept for high-speed liquid chromatography[J].J.Chromatogr.,1974,99:23-30
[7]Otsuka S,Listowsky I.High-resolution preparative electrochromatography for purification of two subunit types of ferritin.Anal.Biochem.1980,102:419-422.
[8]Jorgenson J W,Lukaes K D.High-resolution separations based on electrophoresis and electroosmosis[J].J.Chromatogr.1981,218:209-216
[9]Tsuda T,Nomura K,Nakagawa G J.Open-tubular microcapillary liquid chromatography with electro-osmosis flow using a UV detector[J].J.Chromatogr.,1982,248:241-247
[10]Knox J H.Miniaturization in pressure and electroendosmotically driven liquid chromatography:some theoretical considerations[J].Chromatographia,1984,24,135-143
[11]Knox J H.Thermal effects and band spreading in capillary electro-separation[J].Chromatographia,1988,26,329-337
[12]Lord G A,Gordon D B,Tetier L W,et.al.Electrochromatography-electrospray mass spectrometry of textile dyes[J].J Chromatogr A.,1995,700:27-33
[13]Fujimoto C.Charged polyacrylamide gels for capillary electrochromatographic separations of uncharged,low molecular weight compounds[J].Anal.Chem.,1995,67(13):2050-2053.
[14]Mohammad J,Zeerak A,Hjerten S.Dye-ligand affinity-chromatographyon continuous beds[J].Biomed.Chromatogr.1995,9:80-84.
[15]Yan C,Dadoo R,Zare R N,Gradient elution in capillary electrochro-matography[J].Anal.Chem.,1996,68,:2726-2730
[16]Choudhary G.,Horvath C,Dynamics of capillary electrochromatography:experimental study on the electroosmotic flow and conductance in open and packed capillaries[J].J.Chromatogr.A.,1997,781:161-183
[17]Pusecher K,Schewitz J,Bayer E.On-line coupling of capillary electr-ochromatography,capillary electrophoresis and capillary HPLC with nuclearmagnetic resonance spectroscopy [J].Anal.Chem.,1998,70:3280-3285
[18]Wen E,Asiaie R,Horwath C.Dynamics of capillary electrochromatography Ⅱ.Comparison of column efficiency parameters in microscale high-performance liquid chromatography and capillary electrochromatography[J].J.Chromatogr.A.,1999,11:263-296
[19]Stol R,Kok-Wim Th,Poppe H.Capillary electrochromatography with macroporous particles [J].J.Chroamtogr.A.,1999,953:45-54
[20]Zhang L,Zhang Y,Zou H F.Properties and applications of mixed packing capillary electrochromatography[J].J.High Resol.Chromatogr.,1999,22:666-670
[21]Ye M,Zou H F,Zhang Y.Capillary electrochromatography with a silica column with a dynamically modified cationic sufactant[J].J.Chromatogr.A,1999,855:137-145
[22]Wanders B J,Van D,Goor A M,Everaerts F M,Methods for on-line determination and control of electroendosmosis in capillary electrochromatography andelectrophoresis[J].J.Chromatogr.,1989,490:89-93
[23]陈义.毛细管电泳技术及应用[M].北京:化学工业出版社,2000.1-78
[24]邓延卓,何金兰.高效毛细管电泳[M].北京,科学出版社,2000年,p24
[25]Ye M L,Zou H F,Ni J Y.Separation of acidic compounds by strong anion-exchange capillary electrochromatography[J].J.Chromatogr.A.,2000,887:223-231
[26]王俊德,商振华,郁蕴璐,高效液相色谱法[M]北京,中国石化出版社,1992年,p12
[27]邹汉法,刘震,叶明亮,张玉奎,毛细管电色谱及其应用[M].北京,科学出版社,2001年,p75
[28]Golay M J E.Vapor phase chromatography and telegrapher's equation[J].Anal.Chem.,1957,29:928-932
[29]Ishii D,Hibi K,Asai K.etal.Studies of microhigh-performance liquid chromatography:Ⅱ.Application to gel permeation chromatography of techniques developed for micro high-perfromance liquid chromatography[J].J.Chromatogr.,1978,151:147-154
[30]尤慧艳,张维冰,张玉奎,等.毛细管电色谱柱制备技术的进展[J].色谱,2005,23(5):464-469
[31]Li S,Lloyd D K.Direct chiral separation by capillary electrophoresis using capillaries packed with an al-acid glycoprotein chiral stationary phase[J].Anal.Chem.,1993,65:3684-3691
[32]Knox J H.Terminology and nomenclature in capillary electroseparation systems[J].J.Chromatpgr.,1994,680:3-13
[33]Tsuda T,Nomura K,Nakagawa G J.Open-tubular microcapillary liquid chromatography with electro-osmosis flow using a UV detector[J]J.Chromatogr.,1982,248:241-247
[34]Martin M,Guioehon G.Axial dispersion in open-tubular capillary liquid chromatography with electroosmotic flow[J].AnaLChem.,1984,56(4):614-620
[35]Martin M,Guioehon G.,Walboel Y,et al.Broadening in open-tubular liquid chromatography with electroosmotic flow[J].Anal.Chem.1985,57(2):559-561
[36]Bruin G.J M,Toek P P H,Krak J C,PoPPe H.Electrically driven open-tubular liquid chromatography[J].J Chromatogr.,1990,517:557-572
[37]Guo Y,Colon L A.A stationary phase for open tubular liquid chromatography and electrochromatography using sol-gel technology[J].Anal.Chem.,1995,67(15):2511-2516
[38]Pfeffer W D,Yeung E S.Open-tubular liquid chromatography with surfactant-enhanced electroosmotic flow[J].Anal.Chem.,1990,62(20):2178-2182
[39]Pfeffer W D.Yeung E S.Electroosmotically driven electrochromatography of anions having similar electrophoretic mobilities by ion pairing[J].J.Chromatogr.A,1991,557:125-136
[40]Mayer S,SchurigV.Enantiomer separation by electrochromatography on capillaries coated with chirasil-dex[J].J.High Resolut Chromatogr&Chromatogr Commun,1992,15:129-131
[41]Mayer S,Schurig V.Enantiomer separation by electrochromatography in open tubular columns coated with chirasil-dex[J].J.Liquid.Chromatogr.,1993,16:915-931
[42]Arnlstrong D W,Tang Y,Ward T,Niehols M.Derivatized cyclodextrins immobilized on fused-silica capillaries for enantiomeric separations via capillary electrophoresis,gas hromatography,or supercritical fluid chromatography[J].Anal.Chem.,1993,65(8):1114-1117
[43]Sehurig V,Jung M,Mayer S,et al.Toward unified enantioselective chromatography with a single capillary column coated with chirasil-dex[J].Angew.Chem.I nt.ED.Eng.,1994,33:2222.-2232
[44]Sehurig V,Jung M,Mayer S,et al.Unified enantioselective capillary chromatography on a Chirasil-DEX stationary phase Advantages of column miniaturization[J].J Chromatogr A,1995,694:119-128
[45]Francotte E,Jung M.Enantiomer separation by open tubular liquidChromatography and electrochromatography in cellulose2coated capillaries[J].Chromatographia,1996,42:521-527
[46]Cikalo M G.,Bartle K D,Myers P.Behavior of Cation-Exchange Materials in Capillary Electrochromatography[J].Anal.Chem.,1999,71(9):1820-1825
[47]Liu Z,Zou H F,Ni J Y,et al.Open tubular capillary electrochromatography with adsorbed stationary phase[J].Anal.Chim.Acta,1999,378:73-76
[48]Liu Z,Zou H F,Ye M L,el at.Study of physically adsorbed stationary phases for open tubular capillary electrochromatography[J].Electrohoresis,1999,20:2891-2897
[49]Liu Z,Otsuka K,Terabe S.Chiral separation by open tubular capillary electrochromatography with adsorbed avidin as a stationary phase[J].J.Sep.Sci.,2001,24(1):17-26
[50]Breadmore M C,Maeka M,Avdalovie N,et al.Open-tubular ion-exchange capillary electrochromatography of inorganic anions[J].Analyst.2000,125:1235
[51]Hibi K,Tsuda T.Studies of open-tubular microcapillary liquid chromatography:Ⅲ.β,β＇-oxydipropionitrile and ethylene glycol stationary phases[J].J.Chromatogr.,1979,175:105-111
[52]Ishii D,Tsuda T,Takeuchi T.Studies of open-tubular micro-capillary liquid chromatography:IV.Soda-lime glass columns treated with alkaline solution[J].J.Chromalogr.,1979,185:73-78
[53]Tsuda T,Tsuboi K,Nakagama G,Open-tubular microcapillary liquid chromatography with 20-μtm I.D.columns[J].J.Chromatogor.,1981,214:283-290
[54]Pesek J J,Matyska M T,Separation of tetracyclines by high-performance capillary electrophoresis and capillary electrochromatography[J].J.Chromatogr.A,1996,736:313-320
[55]Pesek J J,Matyska M T,Mauskar L.Separation of proteins and peptides by capillary electrochromatography in diol-and octadecyl-modified etched capillaries[J].J Chromatogr A,1997,763:307-314
[56]Pesek J J,Matyska M T,Cho S J.Open tubular capillary electrochromatography in etched,chemically modified 20 μtm I.D.capillaries[J].J.Chrolnalogr.A,1999,845:237-246
[57]Matyska M T,Pesek J.J,Katrekar A.Open tubular capillary electrochromatography using etched fused-tilica tubing modified with chemically bonded liquid crystals[J].Anal.Chem.,1999,71:5508-5514
[58]Matyska M T,Pesek J J,Boysen R I.Electrochromatographic characterization of etched chemically-modified capillaries with small synthetic peptides[J].J.Chromatogr A,.2001,924:211-221
[59] Catabay A P, Sawada H, Jinno K, et al. Determination of cefobid using adsorptive stripping voltammetric technique in different media [J]. Electrophoresis, 1998, 5 (1): 85-89
[60] Guan N, Zeng Z R, Wang Y C. Open tubular capillary electrochromatography in fused-silica capillaries chemically bonded with macrocyclic dioxopolyamine [J]. Anal. Chim. Acta, 2000, 418: 145-151.
[61] Toek P P H, Boshoven C, Poppe H. Performance of porous silica layers in open-tubular columns for liquid chromatography [J]. J. Chromatogr. A, 1989,477: 95-106
[62] Bruin G J M, Toe K P H, Kraak J C. Electrically driven open-tubular liquid chromatography [J]. J. Chromatogr. A, 1990, 517: 557-572
[63] Crego A L, Diezmasa J C, Dabrio M V. Preparation of open tubular columns for reversed-phase liquid chromatography[J]. Anal. Chem., 1993, 65: 1613-1624
[64] Narang P,Col6n L A.Sol-gel derived fluorinated stationary phase for open tubular capillary electrochromatography[J], J. Chromatogr. A, 1997,773: 65-72
[65] Freitag R,Constantin s. Investigation of factors influencing the performance of open-tubular stationary phases in capillary electrochromatography [J]. J. Sep. Sci.2003, 26: 835-843
[66] Constantin S,Freitag R,Solignac D,Sayah A,Gijs M A M.Utilization of the sol-gel technique for the development of novel stationary phases for capillary electrochromatography on a chip[J]. Sen. Actuat. B 2001,78: 267-272
[67] Rodriguez S A, colon L A. Investigations of a sol-gel derived stationary phase for open tubular capillary electrochromatography [J]. Anal. Chim. Acta, 1999, 397: 207-215
[68] Constantin S, Freitag R. Preparation of stationary phases for open-tubular capillary electrochromatography using the sol-gel method [J] J. Chromatogr. A, 2000, 887: 253-263
[69] Hayes J D, Malik A. Sol-gel open tubular ODS columns with reversed electroosmotic flow for capillary electrochromatography [J]. Anal. Chem., 2001,73: 987-996
[70] Huang X, Zhang J, Horva'th C. Capillary electrochromatography of proteins and peptides with porous layer open-tubular columns [J]. J. Chromatogr. A, 1999, 858: 91-101
[71] Xu W S, Regnier F E. Electrokinetically-driven cation-exchange chromatography of proteins and its comparison with pressure-driven high-performance liquid chromatography [J]. J. Chromatogr. A, 1999, 853: 243-256
[72] Liu Z, Zou H F, Ni J Y. Open tubular capillary electrochromatography with adsorbed stationary phase[J]. Anal. Chim. Acta., 1999, 378: 73-76
[73] Charvatova J, KasickaV, Kral V, et al. Capillary electrochromatographic study of the interactions of porphyrin derivatives with amino acids and oligopeptides [J]. J. Chromatogr. B, 2002, 770: 165-175
[74] Charvatova J, KasickaV, Deyl Z, et al. Open tubular capillary electrochromatography of uuderivatized amino acids using Rh (III) tetrakis (phenoxyphenyl) porphyrinate as wall modifier [J]. J. Chromatogr. A., 2003, 990: 159-167
[75] Shiue C C, Lin S Y, Liu C Y. Chemically bonded fulleredn C_(60) capillary column for the electrophoretic separation of plant phenols [J]. Chin. J. Chem. Soc, 2001,48: 1029-1034
[76] Zeng Z R, Xie C H, Li H B, et al. Open-tubular capillary electrochromatography using capillary columns chemically bonded with the new host molecules calix [6] crown, calix [6] arene [J]. Electrophoresis, 2002, 23: 1272-1278
[77] Wu X J, Liu H X., Liu H, et al. Preparation and characterization of ptertbutylcalix [8] arene bonded capillaries for open-tubular capillary electrochromatography [J]. Anal. Chim. Acta., 2003,478:191-197
[78]Mayer S,Schurig V.Enantiomer separation by eletrochromatography in open tubular columns coated with chirasil-dex[J].J.Liq.Chromatogr.,1993,16(4):915-931
[79]Li S,Lloyd D K.Direct chiral separation by capillary electrophoresis using capillaries packed with an al-acid glycoprotein chiral stationary phase[J].Anal.Chem.,1993,65:3684-3691
[80]Lloyd D K,Li S,Ryan P.Protein chiral selectors in free-solution capillary electrophoresis and packed-capillary electrochromatography[J].J.Chromatogr.A,1995,694:285-296
[81]Zhao Y X,Zhao R,Shangguan D H,e tal.A new type of capillary column for open-tubular electrochromatography[J].Electrophoresis,2002,23(17):2990-2995
[82]Pfeffer W D,Yeung E S.Electroosmotically driven electrochromatography of anions having similar electrophoretic mobilities by ion pairing[J].J.Chromatogr.A,1991,557:125-136
[83]Schweitz L,Andersson L I,Nilsson S.Capillary electrochromatography with predetermined selectivity obtained through molecular imprinting[J].Anal.Chem.,1997,69(6):1179-1183
[84]Wu J T,Huang P,Li M X,et al.Open-tubular capillary electrochromatography with an on-line ion trap storage/reflectron time-of-flight mass detector for ultrafast peptide mixture analysis[J].Anal.Chem.,1997,69(3):320-326
[85]曾昭睿,谢传辉,王园朝,等.溶胶-凝胶法制备丙二酰胺型二氧大环多胺用作开管柱电色谱固定相的研究[J].高等学校化学学报,2001,22(7):1108-1110
[86]Kapnissi C P,Valle B C,Warner I M.,et al.Chiral separations using polymeric surfactants and polyelectrolyte multilayers in open-tubular capillary electrochromatography[J].Anal.Chem.,2003,75(22):6097-6104
[87]Bhleier E,Wehner W,Vogtle F."Casad" and "Nonskid-chain-like" Syntheses of Moleeular Cavity Topologies[J].Synthesis,1978,2:155-158
[88]Deenkewalter R G,Kole J,LukasavgaeW J.Moeormoleeular highly barnehed homogeneous compound based on lysine units[P].US:4289 872,1981-07
[89]Tomalia D A,Bkaer H,Deward J,et al.A new classof polymesr:sartbusrt-dendritie macromoleeules[J].Polym.J.1985,17:117-132
[90]Newkome G R,Yao Z Q,Bkaer G R,et al.Casedae moleeules:A new approach to mieelles a arboorl[J].J.Ogr.Chem.,1985,50:2003-2004
[91]Hawker C J,Freehet J M J.Preparation of polymers with contorlled moleeular aerhiteetuer.A new convergent approaeh to dendritic macromoleeules[J].J.Am.Chem.Soc.,1990,112:7638-7647
[92]Serroni S,Denci G,Gapgaha S.Arborist form luminescent and redox-actjve transition metal complex fragments[J].Angew.Chem.hnt.Ed.Eng.,1992,31(11):1493-149
[93]Vogtle F,Gestermann S,Hesse R,et al.Functional dendrimers[J].J.Prog.Polym.Sci.,2000,25:987-1041
[94]Lothian-Tomalia M K,Hedstrand D M,Tomalia D A.The divergent synthesis of poly(thioether) dendrimers.amplified,genealogically directed synthesis leading to the de gennes dense packed state[J].Tetrahedron,1997,53:15495-15513
[95]Furka A,Sebastyen F,Asgedom M,et al.General method for rapid synthesis of multicomponent peptide mixtures[J].J.Pept.Protein.Res.,1991,37:487-493
[96]Haag R.Dendrimers and hyperbranched polymers as high-loading supports for organic synthesis[J].Chem.Eur.J.,2001,7:327-335
[97]Lebreton S,Monaghan S,Bradley M.Solid-phase dendrimer chemistry:synthesis and application[J].J.Aldrichimica.Acta.,2001,34(3):75-83
[98]Genderen M H P,Baars M W P L,Hest J C M,et al.Obser vingin dividual generationsin poly(propyleneimine) dendrimers withnatural-abundancel(15)~N-NMR spectroscopy[J].J.Reel.Trav.Chim.Pays-Bas.,1994,113:573-574
[99]吴文娟,徐冬梅,张可达,等.聚酰胺-胺树状大分子的应用[J].高分子学报,2003,8:67-71
[100]赵立文,范青华,周海峰,等.树状大分子在有机合成中的应用[J].化学进展,2006,18(2):317-330
[101]Valerio C,Alonso E,Ruiz J,et al.A polycationic metallodendrimer with 24 organoiron termini which senses chlorideand bromide anions[J].Angew.Chem.Int.Ed.Engl,1999,38:1747-175
[102]Tanaka N,Fukutome T,Tanigawa T,et al.Structural selectivity provided by starburst dendrimers as pseudostationary phase in electrokinetie chromatography[J].J.Chromatogr A,1995,699:331-341
[103]Shi X,Lesniak W,Islam MT,et al.Comprehensive characterization of surfaee-functionalized poly(amidoamine) dendrimers with acetamide,hydroxyl,and carboxyl groups[J].Colloid.Surface.A:Physicochem.Eng.Aspects,2006,272:139-150
[104]Shi X,Banyai I,Rodriguez K,etal.Electrophoretic mobility and molecular distribution studies of poly(amidoamine) dendrimers of defined charges[J].Electrophoresis,2006,27:1758-1767
[105]Chao H C,Hanson J E.Dendritic polymers as bonded stationary phase's incapillary electrochromatography[J].J.Sep.Sci.,2002,25:345-350
[106]张志胜,杨屹.以树形大分子聚酰胺-胺(PAMAM)为键合固定相的开管毛细管电色谱柱的制备及评价[J].高等学校化学学报,2006,27(1):47-51
[107]郭宝晶,杨屹,苏萍.牛血清白蛋白-PAMAM修饰的开管毛细管电色谱柱的制备[J].高等学校化学学报,2007,28(7):1267-1269
[108]王静,姜凤超.微波有机合成反应的新进展[J].有机化学,2002,22(3):212-219
[110]Gedye R,Snuth F,Westaway K,et al.The use of microwave ovens for rapid organic synthesis[J].Tetrahedron.Lett.,1986,27:279-282
[111]Giguere R.J,Bray T L,Duncan S M,et al.Application of commercial microwave ovens to organic synthesis[J].Tetrahedron.Lett.,1986,27:4945-4948
[112]Vanderhoff J W.Carrying out chemical reactions using microware[P].US 3432413.1969-03-11
[113]孟庆华,陈虹锦,方能虎,等.面向化学研究的微波反应器[J].实验室研究与探索,2003,22(3):94-97
[114]Willging E M.Microware digestion techniques in the sequential extraction techniques in the sequential extraction of calcium,iron,chromium,manganese,leasd and zinc in sedimenes [J].Anal.Chem.,1987,59(7):938-945
[115]梅乐和,岑沛霖.现代酶工程.化学工业出版社[M].2002,92-1451
[116]w Aehle,林章凛,李爽.工业酶—制备与应用[M].化学工业出版社,2002,22-85
[117]马俊锋,段继诚,梁振,等.固定化酶反应器及其在蛋白质组研究中的应用[J].分析化学,2006,34(11):1649-1655
[118]肖海军,贺筱蓉.固定化酶及其应用研究进展[J].生物学通报,2001,36:9-10
[119]刘建龙,王瑞明,刘建军,等.酶的固定化技术研究进展[J].中国酿造,2005,9:4-6
[120]Yang J,Hage D S.Chiral separations in capillary electrophoresis using human serum albumin as a buffer additive[J].Anal.Chem.,1994,66:2719-2725
[121]Charvatova J,Deyl Z,Kasicka V,dt al.Open tubular capillary electrochromatography of underivatized amino acids using Rh(Ⅲ) phenoxyphenyl porphyrinate as wall modifier[J].J.Chromatogr.A,2003,990:159-167
[122]Ellerby L M,Nishida CR,Nishida F,et al,Encapsulation of proteins in transparent porous silicate glasses prepared by the sol-gel method[J].Science,1992,255:1113-1115
[123]Edmiston P L,Wambolt C L,Smith M K.Spectroscopic characterization of albumin and myoglobin entrapped in bulk sol-gel glasses[J].Colloid.Interface.Sci.,1994,163:395-406
[124]Braun S,Rappoport S,Zusman R.Biochemically active sol-gel glass:the trapping of enzymes[J].J.Mater.Lett.,1990,10(1-2):1-5
[125]Miller S A,Hong E D and Wright D.Rapid and Efficient enzyme encapsulation in a dendrimer silica nanocompositea[J].Macromol.Biosci.,2006,6:839-845
[126]Flora K.Brennan J D.Fluorometric detection of Ca~(2+) based on anInduced change in the conformation of sol-gel entrapped parvalbumin[J].Anal.Chem.,1998,70:4505-4513
[127]Svobodova L,Snejdarkova M,Hianik T.Properties of glucose biosensors based on dendrimer layers.Effect of enzyme immobilization[J].Anal.Bioanal.Chem.,2002,373:735-741
[128]吴红,姜忠义.溶胶-凝胶法包埋生物活性分子[J].现代化工,2002,22(7):60-65
[129]Minakuchi H,Nakanishi K,Soga N,Ishizuka N,et al.Octadecylsilylated porous silica rods as separation media for reversed-phase liquid chromatography[J].Anal.Chem.,1996,68:3498-3501
[130]Ishizuka N,Minakuchi H,Nakanishi K,etal.Performance of a monolithic silica column in a capillary under pressure-driven and electrodriven conditions[J].Anal.Chem.,2000,72:1275-1280
[131]Birnbaumt S,Nilsson S.Protein-based capllary affinity gel electropgoresis for the separation of optbal Isomers[J].Anal.Chem.,1992,64:2872-2874
[132]Licklider L,Kuhr W.Optimization of on-line peptide mapping by capillary zone electrophoresis[J].Anal.Chem.,1994,66:4400-4407
[133]Licklider L,Kuhr W G,Lacoy M P.On-line microreactors/capillary electrophoresis/Mass spectrometry for the analysis of proteinsand peptides[J].Anal.Chem.,1995,67:4170-4177
[134]Ye M,Hu S,Schoenherr RM.On-line protein digestion and peptide mapping bycapillary electrophoresis with post-column labeling for laser-induced fluorescence detection[J].Electrophoresis,2004,25:1319-1326
[135]Sakai-Kato K,Kato M,Toyo'oka T.On-Line Trypsin-encapsulated enzyme reactor by the sol-gel method integrated into capillary electrophoresis[J].Anal.Chem.,2002,74:2943-2949
[136]Kato M,Sakai-KatoK,Jin H M,etal.Integration of on-line protein digestion,peptide separation,and protein identification usingpepsin-coated photopolymerized sol-gel colunm sand capillary electrophoresis/mass spectrometry[J].Anal.Chem.,2004,76:1896-1902
[137]Amankwa LN,Kuhr WG.Trypsin-modified fused-silica capillary microreactor for peptidemapping by capillary zone electrophoresis[J].Anal.Chem.,1992,64:1610-1613
[138]Krvenkova J,Bilkova Z,Foret F.Characterization of a monolithic immobilized trypsin microreactor with on-line coupling to ESI-MS[J].J.Sep.Sci.,2005,28:1675-1684
[139]Sakai-Kato K,Kato M,Toyo'oka T.On-Line Trypsin-Encapsulated Enzyme Reactor by the Sol-Gel Method Integrated into Capillary Electrophoresis[J].Anal.Chem.,2002,74:2943-2949
[1]Xu L,Sun Y.Fabrication and characterization of open tubular CEC modified with tentacle-type metal-chelating polymer chains[J].Electrophoresis,2007,28:1658-1667
[2]Eeckhaut A V,Michotte Y.Chiral separations by capillary electrophoresis:Recent developments and applications[J].Electrophoresis,2006,27:2880-2895
[3]Jorgenson J W,Lukacs K D.Zone electrophoresis in open-tubular glass capillaries[J].Anal.hem.,1981,53:1298-1302
[4]Terabe S,Otsuka K,Ichikawa K,et al.Electrokinetic separations with micellar solutions and open-tubular capillaries,(Direct analysis of thin-layer chromatography spots by fast atom bombardment mass spectrometry)[J].Anal.Chem.,1984,56:111-113
[5]Jingwu Kang,Dorothee Wistuba,Volker Schurig,Recent progress in enantiomeric separation by capillary electrochromatography[J].Electrophoresis,2002,23:4005-4021
[6]寿崇琦,邢希学,康杰分等.有机硅树枝状大分子涂层毛细管电泳柱的制备及性能评价[J].分析化学,2008,36(2):167-171
[7]Tomalia D A,Berry.V,Hall M,et al.Communications to the Editor[J].Macromolecules,1987,20:1164-1167
[8]Shi X,Majoros I J,Patri A K,et al.Molecular heterogeneity analysis of poly(amidoamine)dendrimer-based mono-and multifunctional nanodevices by capillary electrophoresis[J].Analyst,2006,131:374-381
[9]Shi X,Majoros I J,Patri A K,et al.Electrophoretic mobility and molecular distribution studies of poly(amidoamine) dendrimers of defined charges[J].Electrophoresis,2006,27:1758-1767
[10]Lebreton S,Monaghan S,Bradley M.Solid-phase dendrimer chemistry:synthesis and applications[J].Aldrichimica Acta,2001,34(3):75-82.
[11]Swali V,Wells N J,Langley G J,et al.Solid-phase dendrimer synthesis and the generation of super-high-loading resin beads for combinatorial chemistry[J].J.Org.Chem,1997,62:4902-4903.
[12]张志胜,杨屹.以枝形大分子聚酰胺-胺(PAMAM)为键合固定相的开管毛细管电色谱柱的制备及评价[J].2006,27(1):47-51
[13]郭宝晶,杨屹,苏萍.牛血清白蛋白2PAMAM修饰的开管毛细管电色谱柱的制备[J].2007,28(7):1267-1269
[14]Gedye R,Smith F,Westanay K,et al.The use of microwave ovens for rapid organic synthesis[J].Tetrahedron Lett,1986,27(3):279-282
[15]Giguere R J,Bray T L,Duncan S M.Application of commercial microwave ovens to organic synthesis[J].Tetrahedron Lett.,1986,27(41):4945-4948
[16]金钦汉.微波化学[M].北京:科学技术出版社,2001.126-146
[17]戈明亮.微波在聚合物科学中的应用[J].合成材料老化与应用,2004,33(2):37-40
[18]倪春梅,盛凤军.微波合成技术及在有机合成中的应用[J].广州化工,2004,32(2):11-14
[19]杨莹丽,于东冬,刘岩,等.合成树枝状高分子聚酰胺-胺及其荧光性质[J].辽宁石油化工大学学报,2006,26(4):56-57
[20]Zhang Y,Ye X,Tian M,etal.Novel method to prepare polystyrene-based monolithic columns for chromatographic and electrophoretic separations by microwave irradiation[J].J. Chromatogr.A,2008,1188:43-49
[21]Wang Y C,Zeng Z R,Guan N,el at.Sol-gel technique for the preparation ofβ-cyclodextrin derivative stationary phase in open-tubular capillary electrochromatography[J].Electrophoresis,2001,22:2167-2172
[22]Lin X,Wang J,Li L,et al.Separation and determination of five major opium alkaloids with mixed mode of hydrophilic/cationexchange monolith by pressurized capillary electrochromatography[J].J.Sep.Sci.2007,30:3011-3017
[23]Imhof A,Megens M,Engelberts J J,et al.Spectroscopy of Fluorescein(FITC) dyed colloidal silica spheres[J].J.Phys.Chem.B 1999,103:1408-1415
[24]Son Y J,Jang J,Cho Y W,et al.Biodistribution and anti-tumor efficacy of doxorubicin loaded glycol-chitosan nanoaggregates by EPR effect,J.Control.Release,2003,91:135-145
[25]Hungerford G,Benesch J,Mano J F,et al.Effect of the labelling ratio on the photophysics of fluorescein isothiocyanate(FITC) conjugated to bovine serum albumin[J],Photochem.Photobiol.Sci.,2007,6,152-158
[26]Onga K K,Jenkins A L,Chengc R,et al.Dendrimer enhanced immunosensors for biological detection[J].Anal.Chim.Acta,2001,444:143-148
[27]Domanski D M,Klajnert B,Bryszewska M,Incorporation of fluorescent probes into PAMAM dendrimers[J],Bioelectrochemistry,2004,63:193-197
[28]Kitagawa F,Inoue K,Hasegawa T,etal.Chiral separation of acidic drug components by open tubular electrochromatography using avidin immobilized capillaries[J].J.Chromatogr.A,2006,1130:219-226
[29]黄坚,陈胜福,曾涛,等.酸碱滴定分析在聚酰胺-胺表征中的应用[J].高分子材料科学与工程,2007,23(4):185-191
[1]G(u|¨)ibitz G,Schmid M G.Chiral separation by capillary electromigration techniques[J].J.Chromatogr.A,2008,1204:140-156
[2]Wang Y,Yin X,Shi M,etal.Probing chiral amino acids at sub-picomolar level based on bovine serum albumin enantioselective films coupled with silver-enhanced gold nanoparticles[J].Talanta,2006,69:1240-1245
[3]张红丽,赵亮,明永飞,等.模拟移动床色谱分离制备手性农药甲霜灵的研究[J].分析实验室,2007,26(10):14-18
[4]陈立仁.液相色谱手性分离[M].北京:科学出版社,2006.
[5]Peng A,Li R,Hu J,et al.Flow rate gradient high-speed counter-current chromatography separation of five diterpenoids from Triperygium wilfordii and scale-up[J].J.Chromatogr.A,2008,1200:129-134
[6]Fu X,Huang L,Gao F,etal,Carboxymethyl chitosan-coated capillary and its application in CE of proteins[J].Electrophoresis,2007,28:1958-1963
[7]Wang Z,Ouyanga J,Baeyens W R G,Recent developments of enantioseparation techniques for adrenergic drugs using liquid chromatography and capillary electrophoresis:A review[J].J.Chromatogr.B,2008,862:1-14
[8]Wahl A,Schnell I,Pyell U.Capillary electrochromatography with polymeric continuous beds synthesized via free radical polymerization in aqueous media using derivatized cyclodextrins as solubilizing agents[J].J.Chromatogr.A,2004,1044:211-222
[9]Li S,Lloyd D K.Direct chiral separations by capillary electrophoresis using capillaries packed with an acid glycoprotein stationary phase[J].Anal.Chem.,1993,65:3684-3690
[10]Fujimura K,Ueda T,Ando T.Retention behavior of some aromatic compounds on chemically bonded cyclodextrin silica stationary phase in liquid chromatography[J].Anal.Chem.,1983,55(3),446-450.
[11]Tanaka M,Kawaguchi Y,Shono T,Retention behaviour of disubstituted benzene isomers on acetylated cyclodextrin stationary phases[J].J.Chromatogr.A,1983,267:285-292
[12]Matyska M T,Pesek J J,Boysen I,et al,Characterization and applications of etched chemically modified capillaries for open-tubular capillary electrochromatography[J].Electrophoresis,2001,22:2620-2628
[13]Wang Y,Zeng Z,Guan N,et al.Sol-gel technique for the preparation of β-cyclodextrin derivative stationary phase in open-tubular capillary electrochromatography[J].Electrophoresis,2001,22:2167-2172
[14]Wang Y,Xiao Y,Tan Y,et al.Click chemistry for facile immobilization of cyclodextrin derivatives onto silica as chiral stationary phases[J].Tetrahedron Lett,2008,49:5190-5191
[15]杨莹丽,于东冬,刘岩,等.合成树枝状高分子聚酰胺-胺及其荧光性质[J].辽宁石油化工大学学报,2006,26(4):56-57
[16]Giguere R J,Bray T L,Duncan S M.Application of commercial microwave ovens to organic synthesis[J].Tetrahedron Lett.,1986,27(41):4945-4948
[17]金钦汉.微波化学[M].北京:科学技术出版社,2001.126-146
[18]Zhang Y,Ye X,Tian M,etal.Novel method to prepare polystyrene-based monolithic columns for chromatographic and electrophoretic separations by microwave irradiation[J].J.Chromatogr.A,2008,1188:43-49
[19]Gong Y,Lee H K,Application of Cyclam-capped β-cyclodextrin-bonded silica particles as a chiral Stationary phase in capillary electrochromatography for enantiomeric separations[J].Anal.Chem.2003,75:1348-1354
[20]Lee M,Sun C M.Traceless synthesis of hydantoin by focused microwave irradiation[J].Tetrahedron Lett.,2004,45:437-440
[21]Vargas-Dlaz M E,Chaco'n-Garia L,Velazquez P,etal.Enantioselective synthesis of 1-alkyl-substituted 1-phenyl-1,2-ethanediols using a myrtenal-derived chiral auxiliary[J],Tetrahedron-Asymmetr,2003,14:3225-3232
[1]肖海军,贺莜蓉.固定化酶及其应用研究进展[J].生物学报,2001,36(7):9-10
[2]Tang Z,Wang T,Kang J.Immobilized capillary enzyme reactor based on layer-by-layer assembling acetylcholinesterase for inhibitor screening by CE[J].Electrophoresis,2007,28:2981-2897
[3]Krenkova J,Kleparnik K,Foret F.Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries[J].J.Chromatogr.A,2007,1159:110-118
[4]郭忠,张清春,雷政登,等.固定化酶纳升微反应器用于痕量蛋白质快速肽谱分析的研究[J].高等学校化学学报,2002,23(7):1277-1280
[5]马俊锋,段继诚,梁振,等.固定化酶反应器及其在蛋白质组研究中的应用[J].分析化学,2006,34:1649-1665
[6]Sakai-Kato K,Kato M,Toyooka T.On-line trypsin-encapsulated enzyme reactor by the sol-gel method integrated into capillary electrophoresis[J].Anal.Chem.,2002,74(13):2943-2949
[7]Kato M,Sakai-Kato M,Matsumoto N,etal.A protein-encapsulation technique by the sol-gel method for the preparation of monolithic columns for capillary electrochromatography[J].Anal Chem,2002,74:1915-1921
[8]Sakai-Kato K,Kato M,Toyooka T.Creation of an on-chip enzyme reactor by encapsulating trypsin in sol-gel on a plastic microchip[J].Anal Chem,2003,75:388-393
[9]Dulay M T,Baca Q J,Zare R N.Enhanced proteolytic activity of covalently bound enzymes in photopolymerized sol-gel[J].Anal.Chem.2005,77:4604-4610
[10]Bauer M,Griengl H,Steiner W,Kinetic studies on the enzyme(S)-hydroxynitrile lyase from Hevea brasiliensis using initial rate methods and progress curve analysis[J],Biotechnol Bioeng,1999,62:20-35
[11]Ye M L.,Hu S,Schoenherr R M,et al,On-line protein digestion and peptide mapping by capillary electrophoresis with post-column labeling for laser-induced fluorescence detection[J].Electrophoresis,2004,25:1319-1326
[12]Krenkova.J,Foret F.Imniobilized microfluidic enzymatic reactors[J].Electrophoresis,2004,25:3550-3563
[13]Amankwa L N,Kuhr W G,Trypsin-modified fused-silica capillary microreactor for peptidemapping by capillary zone electrophoresis[J].Anal Chem,1992,64:1610-1613
[14]Tang Z M,Kang J W.Enzyme inhibitor screening by capillary electrophoresis with an on-column immobilized enzyme microreactor created by an ionic binding technique[J].Anal Chem.,2006,78:2514-2520
[15]Nashaben W,Rassi Z E.Enzymophoresis of nucleic acids by tandem capillary enzyme reactor-capillary zone electrophoresis[J].J Chromatogr.,1992,596:251-264
[16]Khandare J J,Jayant S,Singh A,et al.Dendrimer versus linear conjugate:influence of polymeric architecture on the delivery and anticancer effect of paclitaxel[J].Bioconjugate chem.,2006,17:1464-1472
[17]Miller S A,Hong E D,Wright D.Rapid and efficient enzyme encapsulation in a dendrimer silica nanocomposite macromolecular bioscience[J].Macromol.Biosci.,2006,6:839-845
[18]Yoon H C,Kim H S.Multilayered assembly of dendrimers with enzymes on gold: thickness-controlled biosensing interface[J].Anal.Chem.,2000,72:922-926
[19]Zhu N N,Gu Y F,Chang Z,et al.PAMAM dendrimers-based DNA biosensors for electrochemical detection of DNA hybridization[J].Electroanalysis,2006,18(21):2107-2114
[20]邓彩,龚跃法.新型树枝状分子复合载体的合成及其对脂肪酶的固定化[J].应用化学,2004,21(10):1024-1028
[1]Reichlew T,Kang S Y,Everhardt D S.The nature of the thermal decomposition of a catalytically active an-ionic claymineral[J].J.Catal.,1986,101(2):352-359
[2]Vaccari A.Clays and catalysis:a promising future[J].Appl.Clay.Sci.,1999,14(4):161-198
[3]Cavani F,Trifiro F,Vaccari C.Hydrotalcite-type anionic clays:Preparation,properties and app lications[J].Catal.toda.,1991,11(2):173-301
[4]孟锦宏,张慧,王治强,等.插层组装超分子结构有机物柱撑阴离子层状材料[J].化学通报,2003.35(2):85-94
[5]Legrouri A,LakraimiM,Barroug A,et al.Removal of the herbicide 2,4-dichlorophenoxyacetate from water to zinc-aluminium-chloride layered double hydroxides [J].Wat.Res.,2005,39(15):3441-3448
[6]Bert F S,Dirk E D V,Mieke B,et al.Transition metal anion exchanged layered double hydroxides as a bioinspired model of vanadium bromop eroxidase[J].J.Catal.,2003,216(122):288-297.
[7]杨巧珍,李峰,段雪.层状双羟基氢氧化物(LDH)的表面有机化研究[J].塑料,2003(3):6-9
[8]景晓燕,郑崇辉,王慧颖,等.水滑石类化合物的研究现状及进展[J].应用科技,2004(10):42-46
[9]吴旭,谢鲜梅,杜亚丽,等.水滑石类材料碱性催化性能的应用研究[J].应用化工,2005(8):469-472
[10]Wang J,Wei M,Rao G,Evans D G,et al.Structure and thermal decomposition of sulfated β-cyclodextrin intercalated in a layered double hydroxide[J].J.Solid.State.Chem.,2004,177:366-371
[11]Zhao Y,Li F,Zhang R,et al.Preparation of layered double-hydroxide nonmaterial with a uniform crystallite size using a new method involving separate nucleation and aging steps[J].J.Solid.State.Chem.,1999,146:506-515
[12]Wei M,Wang J,He J,et al.In situ FI-IR,in situ HT-XRD and TPDE study of thermal decomposition of sulfated-β-cyclodextrin intercalated in layered double by droxides[J].Micropor.Mesopor.Mat.,2005:53-61
[13]Hasegawa J,Masahiro S,Satoru T,et al.High-yield production of optically active 1,2-dios from the corresponding race mates by microbial stereo inversion[J].Agric.Biol.Chem.,1990,54(7):1819-1827.
[14]欧阳健,徐岩,穆晓清.加强辅酶循环再生实现不对称合成(S)-1-苯基-1,2-乙二醇[J].四川大学学报(自然科学版),2007,44(4):561-866
[15]Hyungdon Y,Yung H Y,Byung K C,et al.Simultaneous synthesis of enantiomerically pure (R)-1,2-phenylethanol and(R)-α-methylbenzylamine from racemic α-methylbenzylamine using ω-transaminase/ alcohol dehydrogenase/ glucose dehydrognease coupling reaction [J].Biotechnol.Letters,2003,25:809-812