栝蒌薤白半夏汤对慢性阻塞性肺疾病大鼠气道炎症干预作用的研究
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摘要
目的:研究栝蒌薤白半夏汤对慢性阻塞性肺疾病(chronic obstructivepulmonary disease,COPD)气道炎症的保护作用及其作用机理。
方法:采用烟熏加气管内注入脂多糖法复制COPD模型,从整体状况及肺组织病理形态学等方面观察栝蒌薤白半夏汤对COPD大鼠的保护作用;对支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)进行细胞学计数和分类检查;采用酶联免疫吸附法检测各组血清和/或BALF中细胞因子(白介素-8(interleukin 8,IL-8)、肿瘤坏死因子-α(Tumornecrosis factor alpha,TNF-α))含量。
结果:1.栝蒌薤白半夏汤可使COPD大鼠整体状况、肺组织病理损害得到改善,作用强度优于或类似于泼尼松,以高剂量组为佳。2.栝蒌薤白半夏汤对COPD大鼠中小气道管壁的增厚有不同抑制作用;栝蒌薤白半夏汤可使COPD大鼠平均肺泡面积明显减少(p＜0.01);栝蒌薤白半夏汤可使大鼠BALF细胞总数、单核细胞及中性粒细胞计数比COPD组明显降低(p＜0.01),作用强度优于或类似于泼尼松,以高剂量组为佳。3.栝蒌薤白半夏汤可不同程度地降低COPD大鼠血清及BALF中IL-8、TNF-α水平(p＜0.01或p＜0.05),且高剂量组对BALF中IL-8、TNF-α作用强度优于泼尼松组。
结论:IL-8、TNF-α是COPD发病的主要细胞因子之一,在COPD气道炎症中起重要作用。栝蒌薤白半夏汤对COPD气道炎症有一定治疗作用,其机理与抑制COPD气道炎症中性粒细胞聚集和活化以及抑制IL-8、TNF-α释放有关。
Objective To observe the protective effects of Gualou Xiebai Banxia Decoction(GXBD) on airway inflammation of chronic obstructive pulmonary disease(COPD),and investigate its mechanism initially.
Methods The rat models of COPD were established by intratracheal instillation of lipopolysaccharide(LPS) twice and exposure to cigarette smoke daily.The effects of GXBD on COPD rats were approached by observing general status(such as body weight,movement and respiration, et al) and observing the changes in pathomorhology under light micrioscope.Total and differential cell counts in bronchoalveolar lavage fluids(BALF) were examined.The levels of interleukin-8 (IL-8),tumor necrosis factor-α(TNF-α) in(BALF) or serum were measured by enzyme-linked immunosorbant assays technique(ELISA).
Results After GXBD treatment,the weight of COPD rats increased significantly,movement increased and breath improved.HE staining demonstrated that the characteristics of these groups were lighter than modol group.The thickness of the middle and small airway wall of COPD rats was inhibited by GXBD.Compared with model group,the average area of alveoli in high and low dosage of GXBD groups decreased significantly(p＜0.01).The numbers of total white cell counts, neutrophils and alveolar monocyte-macrophage in BALF after GXBD intervention were significantly lower than COPD rats(P＜0.01).The levels of IL-8,TNF-αin BALE or serum were decreased in the high and low dosage of GXBD groups than COPD group(P＜0.01 or p＜0.05).
Conclusion IL-8 and TNF-αmight play important roles in the airway inflammation of COPD.Gualou Xiebai Banxia Decoction might partly prevent the development of airway inflammation in COPD by inhibiting the neutrophils accumulation in the lung and reducing the release of IL-8 and TNF-α.
方法:采用烟熏加气管内注入脂多糖法复制COPD模型,从整体状况及肺组织病理形态学等方面观察栝蒌薤白半夏汤对COPD大鼠的保护作用;对支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)进行细胞学计数和分类检查;采用酶联免疫吸附法检测各组血清和/或BALF中细胞因子(白介素-8(interleukin 8,IL-8)、肿瘤坏死因子-α(Tumornecrosis factor alpha,TNF-α))含量。
结果:1.栝蒌薤白半夏汤可使COPD大鼠整体状况、肺组织病理损害得到改善,作用强度优于或类似于泼尼松,以高剂量组为佳。2.栝蒌薤白半夏汤对COPD大鼠中小气道管壁的增厚有不同抑制作用;栝蒌薤白半夏汤可使COPD大鼠平均肺泡面积明显减少(p＜0.01);栝蒌薤白半夏汤可使大鼠BALF细胞总数、单核细胞及中性粒细胞计数比COPD组明显降低(p＜0.01),作用强度优于或类似于泼尼松,以高剂量组为佳。3.栝蒌薤白半夏汤可不同程度地降低COPD大鼠血清及BALF中IL-8、TNF-α水平(p＜0.01或p＜0.05),且高剂量组对BALF中IL-8、TNF-α作用强度优于泼尼松组。
结论:IL-8、TNF-α是COPD发病的主要细胞因子之一,在COPD气道炎症中起重要作用。栝蒌薤白半夏汤对COPD气道炎症有一定治疗作用,其机理与抑制COPD气道炎症中性粒细胞聚集和活化以及抑制IL-8、TNF-α释放有关。
Objective To observe the protective effects of Gualou Xiebai Banxia Decoction(GXBD) on airway inflammation of chronic obstructive pulmonary disease(COPD),and investigate its mechanism initially.
Methods The rat models of COPD were established by intratracheal instillation of lipopolysaccharide(LPS) twice and exposure to cigarette smoke daily.The effects of GXBD on COPD rats were approached by observing general status(such as body weight,movement and respiration, et al) and observing the changes in pathomorhology under light micrioscope.Total and differential cell counts in bronchoalveolar lavage fluids(BALF) were examined.The levels of interleukin-8 (IL-8),tumor necrosis factor-α(TNF-α) in(BALF) or serum were measured by enzyme-linked immunosorbant assays technique(ELISA).
Results After GXBD treatment,the weight of COPD rats increased significantly,movement increased and breath improved.HE staining demonstrated that the characteristics of these groups were lighter than modol group.The thickness of the middle and small airway wall of COPD rats was inhibited by GXBD.Compared with model group,the average area of alveoli in high and low dosage of GXBD groups decreased significantly(p＜0.01).The numbers of total white cell counts, neutrophils and alveolar monocyte-macrophage in BALF after GXBD intervention were significantly lower than COPD rats(P＜0.01).The levels of IL-8,TNF-αin BALE or serum were decreased in the high and low dosage of GXBD groups than COPD group(P＜0.01 or p＜0.05).
Conclusion IL-8 and TNF-αmight play important roles in the airway inflammation of COPD.Gualou Xiebai Banxia Decoction might partly prevent the development of airway inflammation in COPD by inhibiting the neutrophils accumulation in the lung and reducing the release of IL-8 and TNF-α.
引文
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[2]程显声,徐希胜,张珍详,等.1992-1999年慢性阻塞性肺疾病、肺心病社区人群综合干预结果[J].中华结核和呼吸杂志,2001,24(10):579-583.
[3]Mueller R,Chanez P,Campbell AM,et al.Different cytokine patterns in bronchial biopsies in asthma and chronic bronchitis.Respir bled,1996,90(2):79-85.
[4]施杞,柯雪帆.现代中医药应用与研究大系[M].第1版,上海:上海中医药大学出版社,1995:139-144.
[5]宋一平,崔德健,茅培英,等.慢性阻塞性肺疾病大鼠模型气道重塑及生长因子的研究[J].中华结核和呼吸杂志,2001,24(5):283-287.
[6]贺石林,王净净,王键.中医科研设计与统计学[M].长沙:湖南科技出版社,2001:48-49
[7]Rubio ML,Sanchez-Cifuentes MV,Ortega M,et al,N-acetylcysteine prevents cigarette smoke induced small airways alterations in rats[J].Eur Respir J,2000,15(3):505-511.
[8]奚肇庆,韩树人,朱佳,等.慢性阻塞性肺病从胸痹论治的临床探讨[J].中医杂志,1990,31(6):35-38.
[9]蔡少杭,吴小玲.胸痹与慢性支气管炎[J].实用中西医结合杂志,1997,10(3):260-261.
[10]魏道祥 栝蒌薤白半夏剂的临床应用探析[J].辽宁中医杂志,2002,29(5):293-294.
[11]江家华.中西医结合治疗肺心病急性发作期39例[J].河南中医,2003,10(6):336
[12]魏道祥.开泄法治疗哮喘101例[J].陕西中医,2003,24(10):877-879.
[13]奚肇庆,蒋萌,居文政,等.复方薤白胶囊治疗慢性阻塞性肺疾病36例临床与实验研究[J].中医杂志,2000,41(4):218-220.
[14]金道学,聂玉英.栝蒌薤白半夏汤加减运用临证二则[J].湖北中医学院学报,2003,5(4):77-78.
[15]王建华,曹世宏,韩树人,等.复方薤白雾化剂雾化吸入结合辨证治疗慢阻肺发作期的临床和实验研究[J].南京中医药大学学报.1991,(4):201-204.
[16]奚肇庆.《金匮要略》胸痹方在呼吸系统疾病中的应用[J].南京中医药大学学报,1998,14(1):39-40.
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