猪血多肽铁螯合盐的制备技术及性质研究
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摘要
缺铁性贫血是体内贮存铁不足,影响血红蛋白合成所引起的一种细胞低色素性贫血,是世界各地贫血中最常见的一种。据WHO报道,全世界约有10%～30%的人群有不同程度的缺铁,男性发病率约10%,女性大于20%。目前,缺铁性贫血的防治主要通过摄入补铁剂来实现。常见的补铁剂有硫酸亚铁、氯化亚铁、葡萄糖酸亚铁、乳酸亚铁、琥珀酸亚铁和富马酸亚铁等,这些补铁剂虽然铁含量高,补铁效果较好,但是由于它们在体内的利用率较低,毒副作用大,并且有特殊的金属铁锈味。多肽螯合铁是蛋白质水解生成的多肽与二价铁离子螯合而生成的生物态铁,在体内不受碳酸盐、单宁酸和纤维素等干扰物质的影响,在体内始终处于可吸收的二价状态,可以直接被肠粘膜细胞吸收,无毒副作用,不产生任何消化道刺激症状,生物利用率高,是一种理想的补铁剂。
我国是世界上生猪出栏数最多的国家,年出栏生猪4～5亿头,每头猪约有2kg猪血,因此每年由屠宰生猪而产生的副产物猪血大约有8～10亿kg,这相当于400万吨猪肉或460万吨全蛋所含的蛋白质。猪血虽然营养丰富,但因其色泽差,血腥味重,适口性差,血红蛋白不易消化吸收,因而未被很好利用。目前,我国的猪血除部分作食用和加工血粉外,还有约70%的猪血被废弃掉,不但浪费了宝贵的资源,而且污染了环境。
因此,本论文以猪血粉为原料,使用碱性蛋白酶进行水解,再将膜分离纯化得到的猪血多肽与亚铁盐进行螯合反应,然后对制得的猪血多肽铁螯合盐进行理化性质研究、功能性质研究和食品毒理学安全性评价,以期为缺铁性贫血人群提供理想的补铁制剂,并为其推广和应用提供理论依据和有益参考。本论文主要获得了以下研究结果:
1、猪血酶法水解制备猪血多肽的研究
1.1 2709碱性蛋白酶对猪血粉具有较好的水解效果,其水解最佳工艺参数为:pH值10.5,水解温度45℃,底物浓度2%,酶与底物质量比3.0%,水解时间6h。
1.2活性炭对猪血粉酶解液具有理想的脱色效果,其最佳脱色工艺参数为活性炭用量2.5%,pH4.0,脱色温度60℃,吸附时间1.0h,在此最佳脱色工艺条件下猪血粉酶解液的脱色率达92.20%,同时氮损失率为10.42%。
1.3离子交换树脂对猪血粉酶解脱色液具有较好的除盐效果。当猪血粉酶解脱色液进样速度为2BV/h时,除盐率达82.11%,同时氮损失率为6.59%。聚丙烯中空纤维超滤膜对除盐后的猪血粉酶解多肽液具有很好的截留分离效果,超滤的最佳操作压力为0.15MPa,多肽液最适温度为25℃,在最佳操作压力和最适温度条件下得到了10kDal以上、5～10kDal、1～5kDal和1kDal以下4个相对分子质量段的猪血多肽,多肽得率分别为4.38%,13.97%,74.76%和6.89%。可见,猪血多肽相对分子质量主要集中于1～5kDal之间。
1.4制备得到的猪血多肽呈浅黄色粉末状,无血腥气味,口感较细腻,味道适中,略带苦味。
2、猪血多肽铁螯合盐的制备及成分分析
2.1从硫酸亚铁和氯化亚铁在不同浓度乙醇中的溶解性可知,氯化亚铁能完全溶于不同浓度的乙醇溶液中,更适合作为螯合反应的铁源。
2.2猪血多肽铁螯合盐制备的最佳工艺参数为:pH值5,多肽与氯化亚铁质量比4:1,多肽溶液浓度3.0%。
2.3通过硫化钠法定性检测螯合反应产物可知,猪血多肽铁是以多肽螯合物形式存在的,是不同于猪血多肽和氯化亚铁的一种新物质,并且其主要成分是多肽和铁,二者质量百分数分别为70.02%和12.23%。
3、猪血多肽铁螯合盐的理化性质研究
3.1猪血多肽铁螯合盐能溶于水中,在中性条件下其溶解度最小,随着酸性或碱性的增强,溶解度增加;猪血多肽铁螯合盐难溶于有机溶剂。
3.2猪血多肽铁螯合盐的外观、固体和溶液的稳定性均优于硫酸亚铁。
3.3经过初步的紫外扫描分析和红外光谱分析可知,猪血多肽铁螯合盐与猪血多肽、氯化亚铁不同,是一种新型螯合物。
4、猪血多肽铁螯合盐的抗氧化和抗贫血功能研究
4.1猪血多肽铁螯合盐能清除超氧阴离子自由基(O_2~-·)和过氧化氢(H_2O_2),维生素C、猪血多肽铁和猪血多肽这三种物质对超氧阴离子自由基(O_2~-·)和过氧化氢(H_2O_2)清除能力的强弱顺序为维生素C＞猪血多肽铁＞猪血多肽,因此猪血多肽铁螯合盐具有较强的清除超氧阴离子自由基(O_2~-·)和过氧化氢(H_2O_2)的能力。
4.2通过低铁饲料喂养,辅以每周尾静脉放血的方法,4周后可以成功建立大鼠缺铁性贫血模型。用猪血多肽铁螯合盐灌胃缺铁性贫血大鼠4周后可使缺铁性贫血大鼠的血红蛋白、红细胞计数、血清铁水平显著升高(P＜0.05),故猪血多肽铁螯合盐可以明显改善大鼠缺铁性贫血,并且其抗贫血效果明显优于葡萄糖酸亚铁和氯化亚铁。
5、猪血多肽铁螯合盐的安全性评价
5.1经口急性毒性(LD_(50))试验表明,小鼠经口灌喂猪血多肽铁螯合盐后无毒性反应,由寇氏改良法计算公式得到猪血多肽铁螯合盐的LD_(50)=30 998mg/kg,大于15000mg/kg,故猪血多肽铁螯合盐为实际无毒物质。
5.2 Ames试验表明,猪血多肽铁螯合盐的5个剂量组在加S-9或不加S-9活化的情况下,4个菌株TA97、TA98、TA100和TA102的回变菌落数与阴性对照组较接近,并且没有超过阴性对照组的2倍,而与阳性对照组相比要低很多,故猪血多肽铁螯合盐不能使鼠伤寒沙门氏菌移码突变株和碱基置换突变株发生回复突变的现象,未呈现致突变性。微核试验和畸形试验表明,阳性对照组(环磷酰胺组)细胞微核率和精子畸形率与阴性对照组相比较,差异具有极显著性(p＜0.01),而猪血多肽铁螯合盐各剂量组细胞微核率和精子畸形率与阴性对照组相比较,差异均不显著(p＞0.05),并且无剂量-效应关系。因此,猪血多肽铁螯合盐对小鼠骨髓细胞染色体无断裂效应,对小鼠精子的畸形无影响。
5.3大鼠30天喂养试验表明,三个剂量试验组的大鼠生长发育与行为活动正常,动物体重和食物利用率与阴性对照组比较均无显著性差异(P＞0.05);各剂量组大鼠血液生化指标与阴性对照组比较均无显著性差异(p＞0.05),且无剂量-效应关系;各剂量组的脏体比与阴性对照组相比较均无显著性差异(p＞0.05),器官组织病理学检查未见异常。
Iron deficiency anemia(IDA),one of the most common anemia in the world,is a kind of cell hypochromic anemia due to insufficient iron which is needed to make hemoglobin.According to WHO,about 10%to 30%of the whole world population are suffered from different degrees of iron deficiency,the incidence of men by about 10%, 20%more than women.At present,prevention and treatment of iron deficiency anemia is mainly achieved through the intake of iron agents such as ferrous sulfate,ferrous chloride,ferrous gluconate,ferrous lactic,ferrous succinate and ferrous fumarate.These agents have good effects in iron supplementation for their high iron content,whereas they can be relatively low utilized in the body,and have side effects and a special taste of metal rust.Polypeptide chelated iron,a biostate iron chelated and generated by polypeptide from albuminolysis and ferrous iron ion,is an ideal agent for it can not be affected by such interferents as carbonate,tannic acid and cellulose,but can always be absorbed directly by intestinal cells without any toxic side effects and irritation.
China is one of the biggest pig producing countries,slaughtering 4 - 500,000,000, pigs each year.And each pig has about 2kg blood,so about 8 to 1,000,000,000 kg pig blood will be produced each year,which is equivalent to the protein content of 4,000,000 tons of pork or 4,600,000 tons of eggs.While the pig blood is nutritious,but because of its poor color,heavy blood smell,poor palatability and non-digestible absorption of hemoglobin,it has not been fully utilised.At present,only less than one third of the pig blood in China is processed as food and blood powder,and there are about 70%of it abandoned.That leads to a waste of valuable resources and environmental pollution.
So,in this paper the pig blood powder has been used to be raw materials,and been hydrolysed by alkaline proteinase,and then the chelating reaction has been conducted between the polypeptides separated and purified by film from the pig blood and ferrite. And then physicochemical properties and functional properties of the chelated pig blood polypeptide-iron salt have been studied,and its toxicological safety has been evaluated with a view to providing a ideal supplementation iron agent for people who are iron deficiency anemia.Finally,the theoretical basis for its promotion and application and a useful reference have been provided.This paper consists of the following findings:
1、Research on preparation of pig blood polypeptides by means of enzymatic hydrolysis
1.1 Concerning hydrolysis,2709 alkaline protease had relatively good effects on pig blood powder,and the optimum hydrolysis technology parameters were:pH value,10.5; hydrolysis temperature,45℃;concentration of substrate,2%;the ratio of enzyme and the substrate quality,3.0%;hydrolysis time,6h..
1.2 Activated carbon had good decolorization effects on the enzymolysed liquid of pig blood powder,and the best decolorization process parameters were:the dosage of activated carbon,2.5%;pH value,4.0;decolorization temperature,60℃;adsorption time, 1.0h.Under the best decolorization conditions the decolorization rate of the enzymolysed liquid of pig blood powder was 92.20%,while the loss rate of nitrogen was 10.42%.
1.3 Ion exchange resins had relatively good demineralization effects on the decolorized enzymolysis liquid of pig blood powder.The demineralization rate was 82.11%when the sample injection speed of decolorized enzymolysis liquid of pig blood powder was 2BV/h,while the loss rate of nitrogen was 6.59%.Polypropylene hollow fiber ultrafiltration membrane had relatively good effects of retention and separation on the demineralized enzymolysis polypeptides liquid of pig blood powder.The optimum operation pressure of ultrafiltration was 0.15MPa,and the optimum temperature of polypeptides solution was 25℃.Under the conditions of optimum operation pressure and optimum temperature the pig blood polypeptides of four relative molecular mass segments were obtained,and they were the segments above 10kDal,5～10kDal,1～5kDal and the segment below 1kDal.The rates of polypeptide yield were 4.38%,13.97 %,74.76%and 6.89%respectively,and the relative molecular mass concentrated on 1～5kDal.
1.4 The yielded pig blood polypeptides are in the form of light yellow powder with delicate mouth.feel and taste,slightly bitter but no smell of blood.
2、Preparation and component analysis of the chelated salt of pig blood polypeptide-iron
2.1 From dissolubility of ferrous sulfate and ferrous chloride in different concentrations of ethanol,we could see that ferrous chloride could be completely dissolved in different concentrations of ethanol solution,and it was more suitable for it to be used as the iron source in the chelation.
2.2 The optimum technology parameters of the preparation of the chelated salt of pig blood polypeptide-iron were:pH value,5;the mass ratio of polypeptides and ferrous chloride is 4:1;the polypeptides solution concentration 3.0%.
2.3 From the qualitation measurement of the chelating reaction products by sodium sulfide method we could see that the pig blood polypeptides iron existed in the form of polypeptides chelate compound,and it was a new material,and it was different from the pig blood polypeptides and ferrous chloride,and its major components were polypeptide and iron,the mass percentages of the two were 70.02%and 12.23% respectively.
3、Study on the physicochemical properties of the chelated salt of pig blood polypeptide-iron
3.1 The chelated salt of pig blood polypeptide-iron could be soluble in water,and under the neutral conditions,it had the minimum solubility,and with the increase of acidity or alkalinity,the solubility rised;and the chelated salt of pig blood polypeptide-iron could not be dissolved in organic solvents.
3.2 The chelated salt of pig blood polypeptide-iron were more stable in appearance and the state of solid and liquid than ferrous sulfate.
3.3 Through a preliminary scan of the ultraviolet and infrared spectroscopy analysis,we could see that the chelated salt of pig blood polypeptide-iron was different from pig blood polypeptide and ferrous chloride,and it was a new type of chelate.
4、The study on the antioxidant and anti-anemia function of the chelated salt of pig blood polypeptide-iron
4.1 The chelated salt of pig blood polypeptide-iron could remove superoxide radical anion(O_2.) and hydrogen peroxide(H_2O_2).The remove ability of the chelated salt of pig blood polypeptide-iron was weaker than that of vitamin C,but stronger than that of pig blood polypeptides,so the chelated salt of pig blood polypeptide-iron had relatively strong ability of removing superoxide radical anion(O_2) and hydrogen peroxide (H_2O_2).
4.2 To establish the model of rat iron deficiency anemia successfully,it involved bloodletting caudal vein each week and feeding it with low iron feed for four weeks. The rat's hemoglobin,the red blood cell count and serum iron levels increased significantly(P＜0.05),fed with the chelated salt of pig blood polypeptide-iron after 4 weeks.It was obvious that the chelated salt of pig blood polypeptide-iron could improve the rat's iron deficiency anemia,and its anti-anemia function was better than that of ferrous gluconate and ferrous chloride.
5、Safety evaluation of the chelated salt of pig blood polypeptide-iron
5.1 Acute oral toxicity(LD_(50)) test showed that the mice had no toxic reaction after being fed with the chelated salt of pig blood polypeptide-iron by mouth.Counted by the improved formula of Karber method the LD_(50) of the chelated salt of pig blood polypeptide-iron was 30 998 mg / kg,and it was more than 15 000mg/kg,so it could be concluded that the chelated salt of pig blood polypeptide-iron was innocuous substance. 5.2 Ames test showed that under the conditions of activating the five groups of different dose by adding S-9 or not,the number of anaplasia bacterial colony of the 4 strains, TA97,TA98,TA100 and TA102,was close to that of the negative control group,and less than 2 times.And compared with positive control group the number was much lower.So it could be concluded that the chelated salt of pig blood polypeptide-iron could not lead to back mutation between the Salmonella typhimurium mutation strain and base replacement mutant,and it had no mutagenicity.Micronucleus and abnormality test showed that the difference in the rate of cell micronuclear and abnormal sperm between the positive control group(fed with cyclophosphamide)and the negative control group was significant(p＜0.01),and that between the negative control group and the groups fed with different dose of the chelated salt of pig blood polypeptide-iron was not significant(p＞0.05),and there's no dose - effect relationship between them.So the chelated salt of pig blood polypeptide-iron had no abruption effect on mice bone marrow cells chromosome,and had no effect on mice abnormal sperm.
5.3 The test of feeding the mice with the chelated salt of pig blood polypeptide-iron for 30 days showed that the growth development and behaviour of mice of the three groups of different dose were normal,and compared with that of the negative control group the differences in body weight and food utilization were not significant(p＞0.05).Compared with that of the negative control group,the differences in blood biochemical indicator were not significantly different(P＞0.05),and there's no dose-effect relationship between them.Compared with that of the negative control group,the differences in the ratio of viscus and corpora were not significant(p＞0.05),and there is no abnormality in the test of organ histopathology.
我国是世界上生猪出栏数最多的国家,年出栏生猪4～5亿头,每头猪约有2kg猪血,因此每年由屠宰生猪而产生的副产物猪血大约有8～10亿kg,这相当于400万吨猪肉或460万吨全蛋所含的蛋白质。猪血虽然营养丰富,但因其色泽差,血腥味重,适口性差,血红蛋白不易消化吸收,因而未被很好利用。目前,我国的猪血除部分作食用和加工血粉外,还有约70%的猪血被废弃掉,不但浪费了宝贵的资源,而且污染了环境。
因此,本论文以猪血粉为原料,使用碱性蛋白酶进行水解,再将膜分离纯化得到的猪血多肽与亚铁盐进行螯合反应,然后对制得的猪血多肽铁螯合盐进行理化性质研究、功能性质研究和食品毒理学安全性评价,以期为缺铁性贫血人群提供理想的补铁制剂,并为其推广和应用提供理论依据和有益参考。本论文主要获得了以下研究结果:
1、猪血酶法水解制备猪血多肽的研究
1.1 2709碱性蛋白酶对猪血粉具有较好的水解效果,其水解最佳工艺参数为:pH值10.5,水解温度45℃,底物浓度2%,酶与底物质量比3.0%,水解时间6h。
1.2活性炭对猪血粉酶解液具有理想的脱色效果,其最佳脱色工艺参数为活性炭用量2.5%,pH4.0,脱色温度60℃,吸附时间1.0h,在此最佳脱色工艺条件下猪血粉酶解液的脱色率达92.20%,同时氮损失率为10.42%。
1.3离子交换树脂对猪血粉酶解脱色液具有较好的除盐效果。当猪血粉酶解脱色液进样速度为2BV/h时,除盐率达82.11%,同时氮损失率为6.59%。聚丙烯中空纤维超滤膜对除盐后的猪血粉酶解多肽液具有很好的截留分离效果,超滤的最佳操作压力为0.15MPa,多肽液最适温度为25℃,在最佳操作压力和最适温度条件下得到了10kDal以上、5～10kDal、1～5kDal和1kDal以下4个相对分子质量段的猪血多肽,多肽得率分别为4.38%,13.97%,74.76%和6.89%。可见,猪血多肽相对分子质量主要集中于1～5kDal之间。
1.4制备得到的猪血多肽呈浅黄色粉末状,无血腥气味,口感较细腻,味道适中,略带苦味。
2、猪血多肽铁螯合盐的制备及成分分析
2.1从硫酸亚铁和氯化亚铁在不同浓度乙醇中的溶解性可知,氯化亚铁能完全溶于不同浓度的乙醇溶液中,更适合作为螯合反应的铁源。
2.2猪血多肽铁螯合盐制备的最佳工艺参数为:pH值5,多肽与氯化亚铁质量比4:1,多肽溶液浓度3.0%。
2.3通过硫化钠法定性检测螯合反应产物可知,猪血多肽铁是以多肽螯合物形式存在的,是不同于猪血多肽和氯化亚铁的一种新物质,并且其主要成分是多肽和铁,二者质量百分数分别为70.02%和12.23%。
3、猪血多肽铁螯合盐的理化性质研究
3.1猪血多肽铁螯合盐能溶于水中,在中性条件下其溶解度最小,随着酸性或碱性的增强,溶解度增加;猪血多肽铁螯合盐难溶于有机溶剂。
3.2猪血多肽铁螯合盐的外观、固体和溶液的稳定性均优于硫酸亚铁。
3.3经过初步的紫外扫描分析和红外光谱分析可知,猪血多肽铁螯合盐与猪血多肽、氯化亚铁不同,是一种新型螯合物。
4、猪血多肽铁螯合盐的抗氧化和抗贫血功能研究
4.1猪血多肽铁螯合盐能清除超氧阴离子自由基(O_2~-·)和过氧化氢(H_2O_2),维生素C、猪血多肽铁和猪血多肽这三种物质对超氧阴离子自由基(O_2~-·)和过氧化氢(H_2O_2)清除能力的强弱顺序为维生素C＞猪血多肽铁＞猪血多肽,因此猪血多肽铁螯合盐具有较强的清除超氧阴离子自由基(O_2~-·)和过氧化氢(H_2O_2)的能力。
4.2通过低铁饲料喂养,辅以每周尾静脉放血的方法,4周后可以成功建立大鼠缺铁性贫血模型。用猪血多肽铁螯合盐灌胃缺铁性贫血大鼠4周后可使缺铁性贫血大鼠的血红蛋白、红细胞计数、血清铁水平显著升高(P＜0.05),故猪血多肽铁螯合盐可以明显改善大鼠缺铁性贫血,并且其抗贫血效果明显优于葡萄糖酸亚铁和氯化亚铁。
5、猪血多肽铁螯合盐的安全性评价
5.1经口急性毒性(LD_(50))试验表明,小鼠经口灌喂猪血多肽铁螯合盐后无毒性反应,由寇氏改良法计算公式得到猪血多肽铁螯合盐的LD_(50)=30 998mg/kg,大于15000mg/kg,故猪血多肽铁螯合盐为实际无毒物质。
5.2 Ames试验表明,猪血多肽铁螯合盐的5个剂量组在加S-9或不加S-9活化的情况下,4个菌株TA97、TA98、TA100和TA102的回变菌落数与阴性对照组较接近,并且没有超过阴性对照组的2倍,而与阳性对照组相比要低很多,故猪血多肽铁螯合盐不能使鼠伤寒沙门氏菌移码突变株和碱基置换突变株发生回复突变的现象,未呈现致突变性。微核试验和畸形试验表明,阳性对照组(环磷酰胺组)细胞微核率和精子畸形率与阴性对照组相比较,差异具有极显著性(p＜0.01),而猪血多肽铁螯合盐各剂量组细胞微核率和精子畸形率与阴性对照组相比较,差异均不显著(p＞0.05),并且无剂量-效应关系。因此,猪血多肽铁螯合盐对小鼠骨髓细胞染色体无断裂效应,对小鼠精子的畸形无影响。
5.3大鼠30天喂养试验表明,三个剂量试验组的大鼠生长发育与行为活动正常,动物体重和食物利用率与阴性对照组比较均无显著性差异(P＞0.05);各剂量组大鼠血液生化指标与阴性对照组比较均无显著性差异(p＞0.05),且无剂量-效应关系;各剂量组的脏体比与阴性对照组相比较均无显著性差异(p＞0.05),器官组织病理学检查未见异常。
Iron deficiency anemia(IDA),one of the most common anemia in the world,is a kind of cell hypochromic anemia due to insufficient iron which is needed to make hemoglobin.According to WHO,about 10%to 30%of the whole world population are suffered from different degrees of iron deficiency,the incidence of men by about 10%, 20%more than women.At present,prevention and treatment of iron deficiency anemia is mainly achieved through the intake of iron agents such as ferrous sulfate,ferrous chloride,ferrous gluconate,ferrous lactic,ferrous succinate and ferrous fumarate.These agents have good effects in iron supplementation for their high iron content,whereas they can be relatively low utilized in the body,and have side effects and a special taste of metal rust.Polypeptide chelated iron,a biostate iron chelated and generated by polypeptide from albuminolysis and ferrous iron ion,is an ideal agent for it can not be affected by such interferents as carbonate,tannic acid and cellulose,but can always be absorbed directly by intestinal cells without any toxic side effects and irritation.
China is one of the biggest pig producing countries,slaughtering 4 - 500,000,000, pigs each year.And each pig has about 2kg blood,so about 8 to 1,000,000,000 kg pig blood will be produced each year,which is equivalent to the protein content of 4,000,000 tons of pork or 4,600,000 tons of eggs.While the pig blood is nutritious,but because of its poor color,heavy blood smell,poor palatability and non-digestible absorption of hemoglobin,it has not been fully utilised.At present,only less than one third of the pig blood in China is processed as food and blood powder,and there are about 70%of it abandoned.That leads to a waste of valuable resources and environmental pollution.
So,in this paper the pig blood powder has been used to be raw materials,and been hydrolysed by alkaline proteinase,and then the chelating reaction has been conducted between the polypeptides separated and purified by film from the pig blood and ferrite. And then physicochemical properties and functional properties of the chelated pig blood polypeptide-iron salt have been studied,and its toxicological safety has been evaluated with a view to providing a ideal supplementation iron agent for people who are iron deficiency anemia.Finally,the theoretical basis for its promotion and application and a useful reference have been provided.This paper consists of the following findings:
1、Research on preparation of pig blood polypeptides by means of enzymatic hydrolysis
1.1 Concerning hydrolysis,2709 alkaline protease had relatively good effects on pig blood powder,and the optimum hydrolysis technology parameters were:pH value,10.5; hydrolysis temperature,45℃;concentration of substrate,2%;the ratio of enzyme and the substrate quality,3.0%;hydrolysis time,6h..
1.2 Activated carbon had good decolorization effects on the enzymolysed liquid of pig blood powder,and the best decolorization process parameters were:the dosage of activated carbon,2.5%;pH value,4.0;decolorization temperature,60℃;adsorption time, 1.0h.Under the best decolorization conditions the decolorization rate of the enzymolysed liquid of pig blood powder was 92.20%,while the loss rate of nitrogen was 10.42%.
1.3 Ion exchange resins had relatively good demineralization effects on the decolorized enzymolysis liquid of pig blood powder.The demineralization rate was 82.11%when the sample injection speed of decolorized enzymolysis liquid of pig blood powder was 2BV/h,while the loss rate of nitrogen was 6.59%.Polypropylene hollow fiber ultrafiltration membrane had relatively good effects of retention and separation on the demineralized enzymolysis polypeptides liquid of pig blood powder.The optimum operation pressure of ultrafiltration was 0.15MPa,and the optimum temperature of polypeptides solution was 25℃.Under the conditions of optimum operation pressure and optimum temperature the pig blood polypeptides of four relative molecular mass segments were obtained,and they were the segments above 10kDal,5～10kDal,1～5kDal and the segment below 1kDal.The rates of polypeptide yield were 4.38%,13.97 %,74.76%and 6.89%respectively,and the relative molecular mass concentrated on 1～5kDal.
1.4 The yielded pig blood polypeptides are in the form of light yellow powder with delicate mouth.feel and taste,slightly bitter but no smell of blood.
2、Preparation and component analysis of the chelated salt of pig blood polypeptide-iron
2.1 From dissolubility of ferrous sulfate and ferrous chloride in different concentrations of ethanol,we could see that ferrous chloride could be completely dissolved in different concentrations of ethanol solution,and it was more suitable for it to be used as the iron source in the chelation.
2.2 The optimum technology parameters of the preparation of the chelated salt of pig blood polypeptide-iron were:pH value,5;the mass ratio of polypeptides and ferrous chloride is 4:1;the polypeptides solution concentration 3.0%.
2.3 From the qualitation measurement of the chelating reaction products by sodium sulfide method we could see that the pig blood polypeptides iron existed in the form of polypeptides chelate compound,and it was a new material,and it was different from the pig blood polypeptides and ferrous chloride,and its major components were polypeptide and iron,the mass percentages of the two were 70.02%and 12.23% respectively.
3、Study on the physicochemical properties of the chelated salt of pig blood polypeptide-iron
3.1 The chelated salt of pig blood polypeptide-iron could be soluble in water,and under the neutral conditions,it had the minimum solubility,and with the increase of acidity or alkalinity,the solubility rised;and the chelated salt of pig blood polypeptide-iron could not be dissolved in organic solvents.
3.2 The chelated salt of pig blood polypeptide-iron were more stable in appearance and the state of solid and liquid than ferrous sulfate.
3.3 Through a preliminary scan of the ultraviolet and infrared spectroscopy analysis,we could see that the chelated salt of pig blood polypeptide-iron was different from pig blood polypeptide and ferrous chloride,and it was a new type of chelate.
4、The study on the antioxidant and anti-anemia function of the chelated salt of pig blood polypeptide-iron
4.1 The chelated salt of pig blood polypeptide-iron could remove superoxide radical anion(O_2.) and hydrogen peroxide(H_2O_2).The remove ability of the chelated salt of pig blood polypeptide-iron was weaker than that of vitamin C,but stronger than that of pig blood polypeptides,so the chelated salt of pig blood polypeptide-iron had relatively strong ability of removing superoxide radical anion(O_2) and hydrogen peroxide (H_2O_2).
4.2 To establish the model of rat iron deficiency anemia successfully,it involved bloodletting caudal vein each week and feeding it with low iron feed for four weeks. The rat's hemoglobin,the red blood cell count and serum iron levels increased significantly(P＜0.05),fed with the chelated salt of pig blood polypeptide-iron after 4 weeks.It was obvious that the chelated salt of pig blood polypeptide-iron could improve the rat's iron deficiency anemia,and its anti-anemia function was better than that of ferrous gluconate and ferrous chloride.
5、Safety evaluation of the chelated salt of pig blood polypeptide-iron
5.1 Acute oral toxicity(LD_(50)) test showed that the mice had no toxic reaction after being fed with the chelated salt of pig blood polypeptide-iron by mouth.Counted by the improved formula of Karber method the LD_(50) of the chelated salt of pig blood polypeptide-iron was 30 998 mg / kg,and it was more than 15 000mg/kg,so it could be concluded that the chelated salt of pig blood polypeptide-iron was innocuous substance. 5.2 Ames test showed that under the conditions of activating the five groups of different dose by adding S-9 or not,the number of anaplasia bacterial colony of the 4 strains, TA97,TA98,TA100 and TA102,was close to that of the negative control group,and less than 2 times.And compared with positive control group the number was much lower.So it could be concluded that the chelated salt of pig blood polypeptide-iron could not lead to back mutation between the Salmonella typhimurium mutation strain and base replacement mutant,and it had no mutagenicity.Micronucleus and abnormality test showed that the difference in the rate of cell micronuclear and abnormal sperm between the positive control group(fed with cyclophosphamide)and the negative control group was significant(p＜0.01),and that between the negative control group and the groups fed with different dose of the chelated salt of pig blood polypeptide-iron was not significant(p＞0.05),and there's no dose - effect relationship between them.So the chelated salt of pig blood polypeptide-iron had no abruption effect on mice bone marrow cells chromosome,and had no effect on mice abnormal sperm.
5.3 The test of feeding the mice with the chelated salt of pig blood polypeptide-iron for 30 days showed that the growth development and behaviour of mice of the three groups of different dose were normal,and compared with that of the negative control group the differences in body weight and food utilization were not significant(p＞0.05).Compared with that of the negative control group,the differences in blood biochemical indicator were not significantly different(P＞0.05),and there's no dose-effect relationship between them.Compared with that of the negative control group,the differences in the ratio of viscus and corpora were not significant(p＞0.05),and there is no abnormality in the test of organ histopathology.
引文
[1]Louise L.Dunn,Yohan Suryo Rahmanto,Des R.Richardson.Iron uptake and metabolism in the new millennium.Trends in Cell Biology,2006,17(2):93-100
[2]Ana B Pérez-Expósito,Salvador Villalpando,Juan A Rivera.et al.Ferrous Sulfate Is More Bioavailable among Preschoolers than Other Forms of Iron in a Milk-Based Weaning Food Distributed by PROGRESA,a National Program in Mexico 1,2.The Journal of Nutrition,2005,135(1):64-70
[3]Philip A.Candela.The evolution of aqueous vapor from silicate melts:Effect on oxygen fugacity.Geochimica et Cosmochimica Acta,1986,50(6):1205-1211
[4]Hua-Min Xu,Hong Jiang,Jun Wang.et al.Over-expressed Human Divalent Metal Transporter 1 is involved in Iron Accumulation in MES23.5 Cells.Neurochemistry International,In Press,Accepted Manuscript,2007.11.9
[5]Conrad ME,Umbreit JN,Moore EG.et al.Separate pathways for cellular uptake of ferric and ferrous iron.Am J Physiol,2000,279(4):G767-G774
[6]Marcel E.Conrad,Jay N.Umbreit.Pathways of Iron Absorption.Blood Cells,Molecules,and Diseases,2002,29(3):336-355
[7]Maria N.Garcia,Carlos Martinez-Torres,Irene Leets.et al.Heat treatment on heme iron and iron-containing proteins in meat:Iron absorption in humans from diets containing cooked meat fractions.The Journal of Nutritional Biochemistry,1996,7(1):49-54
[8]Levine DS,Huebers HA,Rubin CE.et al.Blocking action of parenteral desferrioxamine on iron absorption in rodents and men.Gastroenterol,1988,95:1242-1248.
[9]Boni RE,Hueh-Boni RA,Gallbraith RA.et al.Tinmesoporphyrin inhibits heme oxygenase activity and heme iron absorption in the intestine.Pharmacol,1993,47:318-329
[10]Yifan Cheng,Elmar Wolf,Mykol Larvie.et al.Single Particle Reconstructions of the Transferrin-Transferrin Receptor Complex Obtained with Different Specimen Preparation Techniques.Journal of Molecular Biology,2006,355(5):1048-1065
[11]Katerina Chvátalová,Iva Slaninová,Lenka Brezinová.et al.Influence of dietary phenolic acids on redox status of iron:Ferrous iron autoxidation and ferric iron reduction.Food Chemistry,2008,106(2):650-660
[12]S.Juanto,R.S.Schrebler,J.O.Zerbino.et al.The influence of different cations on the eletrochemical and ellipsometric behaviour of iron in alkaline media.Electrochiraica Acta,1991,36(7):1143-1150
[13]Mirjana Andjelkovic,John Van Camp,Bruno De Meulenaer.et al.Iron-chelation properties of phenolic acids bearing catechol and galloyl groups.Food Chemistry,2006,98(1):23-31
[14]Tomas Ganz,Elizabeta Nemeth.Regulation of iron acquisition and iron distribution in mammals.Biochimica et Biophysica Acta(BBA) - Molecular Cell Research,2006,1763(7):690-699
[15]Pauline T.Lieu,Marja Heiskala,Per A.Peterson.The roles of iron in health and disease.Molecular Aspects of Medicine,2001,22(1-2):1-87
[16]Teresa Olczak,Waltena Simpson,Xinyan Liu.et al.Iron and heme utilization in Porphyromonas gingivalis.FEMS Microbiology Reviews,2005,29(1):119-144
[17]Dachun Yao,Weibin Shi,Yulan Gou.et al.Fatty acid-mediated intracellular iron translocation:A synergistic mechanism of oxidative injury.Free Radical Biology and Medicine,2005,39(10):1385-1398
[18]Mikio Nakamura,Yoshiki Ohgo,Akira Ikezaki.Electronic ground states of low-spin iron(Ⅲ)porphyrinoids.Journal of Inorganic Biochemistry,In Press,Accepted Manuscript,2007
[19]Jana Gerber,Roland Lill.Biogenesis of iron-sulfur proteins in eukaryotes:components,mechanism and pathology.Mitochondrion,2002,2(1):71-86.
[20]Heiko B(o|¨)nisch,Christian L.Schmidt.et al.The Structure of the Soluble Domain of an Archaeal Rieske Iron-Sulfur Protein at 1.1A Resolution.Journal of Molecular Biology,2002,319(3):791-805
[21]中国营养学会.中国居民膳食营养素参考摄入量(Chinese DRIs).北京:中国轻工业出版社,2000
[22]Craig S.Broberg,Bridget E.Bax,Darlington O.Okonko.et al.Blood Viscosity and its Relationship to Iron Deficiency,Symptoms,and Exercise Capacity in Adults With Cyanotic Congenital Heart Disease.Journal of the American College of Cardiology,2006,48(2):356-365
[23]John W.Eaton,Mingwei Qian.Molecular bases of cellular iron toxicity.Free Radical Biology and Medicine,2002,32(9):833-840
[24]Yuhta Shiono,Shinya Wakusawa,Hisao Hayashi.et al.Iron accumulation in the liver of male patients with Wilson's disease.The American Journal of Gastroenterology,2001,96(11):3147-3151
[25]Srdjan Denic,Mukesh M.Agarwal.Nutritional iron deficiency:an evolutionary perspective.Nutrition,2007,23(7-8):603-614
[26]Philip Lanzkowsky.Iron-deficiency anemia.Manual of Pediatric Hematology and Oncology(Fourth Edition),2005
[27]C.Poitou Bernert,C.Ciangura,M.Coupaye.et al.Nutritional deficiency after gastric bypass:diagnosis,prevention and treatment.Diabetes & Metabolism,2007,33(1):13-24
[28]Yuan-Ji Pan,George Loo.Effect of copper deficiency on oxidative DNA damage in Jurkat T-lymphoeytes.Free Radical Biology and Medicine,2000,28(5):824-830
[29]Joao Banha,Liliana Marques,Rita Oliveira.et al.Ceruloplasmin expression by human peripheral blood lymphocytes:A new link between immunity and iron metabolism.Free Radical Biology and Medicine,In Press,Corrected Proof,2007
[30]Vivien M.Hodges,Susan Rainey.et al.Pathophysiology of anemia and erythrocytosis.Critical Reviews in Oncology/Hematology,2007,64(2):139-158
[31]Victoria Arija,Griselda Esparó,Joan Fernández-Ballart.et al.Nutritional status and performance in test of verbal and non-verbal intelligence in 6 year old children.Intelligence,2006,34(2):141-149.
[32]Jerome Y.Yager,Dawn S.Hartfield.Neurologic manifestations of iron deficiency in childhood.Pediatric Neurology,2002,27(2):85-92
[33]Jehn-Shan Yeh,Ching-Hsue Cheng.Using hierarchical soft computing method to discriminate microcyte anemia.Expert Systems with Applications,2005,29(3):515-524
[34]Robert T.Brown,Susan M.Mcintosh,Vicki R.et al.Iron status of adolescent female athletes.Journal of Adolescent Health Care,1985,6(5):349-352
[35]Kieran E.Fallon.Screening for haematological and iron-related abnormalities in elite athletes-Analysis of 576 cases.Journal of Science and Medicine in Sport,In Press,Corrected Proof,2007
[36]Kelly W.Mercer,John J.Densmore.Hematologic Disorders in the Athlete.Clinics in Sports Medicine,2005,24(3):599-621
[37]Beard J,Borel M J,Peterson F J.Change in iron status during weight loss with very-low energy diets.Clinic Nutrition,1997,66(1):104-110
[38]Shane R.Cunha,Peter J.Mohler.Cardiac ankyrins:Essential components for development and maintenance of excitable membrane domains in heart.Cardiovascular Research,2006,71(1):22-29
[39]Richard Shingles,Marisa North,Richard E.McCarty.Direct Measurement of Ferrous Ion Transport across Membranes Using a Sensitive Fluorometric Assay.Analytical Biochemistry,2001,296(1):106-113
[40]Maria Kapsokefalou,Vasilis Kakouris,Kyriakos Makris.et al.Oxidative activity and dialyzability of some iron compounds under conditions of a simulated gastrointestinal digestion in the presence of phytate.Food Chemistry,2007,101(1):419-427
[41]Alexandra Kiskini,Konstantina Argiri,Michael Kalogeropoulos.et al.Sensory characteristics and iron dialyzability of gluten-free bread fortified with iron.Food Chemistry,2007,102(1):309-316
[42]Oscar Pineda,H.DeWayne Ashmead.Effectiveness of treatment of iron-deficiency anemia in infants and young children with ferrous bis-glycinate chelate.Nutrition,2001,17(5):381-384
[43]Fernando Pizarro,Raúl Uicich,Manuel Olivares.et al.Iron absorption of ferric glycinate is controlled by iron stores.Nutrition Research,1998,18(1):3-9
[44]Hiroshi Fujii.Electronic structure and reactivity of high-valent oxo iron porphyrins. Coordination Chemistry Reviews,2002,226(1-2):51-60
[45]B.Gulbis,F.Cotton,F.Vertongen.Hémoglobines anormales rares.EMC -Hématologie,2004,1(4):106-114
[46]Tracey A.Rouault.The Intestinal Heme Transporter Revealed.Cell,2005,122(5):649-651
[47]钟才云,马凤楼,蔡凤鸣.血红素铁治疗女大学生缺铁性贫血的研究.中国校医,1995,9(3):167-169
[48]陈苏陵,唐明龙.从猪血中制备血红素铁及血红素铁强化食品纠正缺铁性贫血疗效分析.桂林医学院学报,1994(1):8-9
[49]钟才云,马凤楼.血红素铁的生物利用研究.营养学报,1989,11(2):135-140
[50]刘雅琴,王德颖,王朝辉等.CMC-血红素铁防治小儿缺铁性贫血的临床研究.医师进修杂志,1999(3):21
[51]J.A.Rendleman.Metal-polysaccharide complexes-Part Ⅰ.Food Chemistry,1978,3(1):47-79
[52]Vlasta Mohacek-Grosev,Romano Bozac,Gerwin J.Puppels.Vibrational spectroscopic characterization of wild growing mushrooms and toadstools.Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2001,5 7(14):2815-2829
[53]Rosalee S.Rasmussen,Michael T.Morrissey.Marine Biotechnology for Production of Food Ingredients.Advances in Food and Nutrition Research,2007,52:237-292
[54]孟凡德,赵全芹,李明霞.紫菜多糖Fe(Ⅲ)配合物的生物利用度初步研究.中国现代应用药学杂志,2002,19(3):207-209
[55]李公斌,王振宇.黑木耳多糖与Fe(Ⅱ)螯合物的制备工艺及红外光谱研究.食品工业科技,2006,27(5):136-138
[56]Roberta J.Ward,Martin J.O'Connell,Dominic P.E.Dickson.et al.Biochemical studies of the iron cores and polypeptide shells of haemosiderin isolated from patients with primary or secondary haemochromatosis.Biochimica et Biophysica Acta(BBA) - General Subjects,1989,993,(1):131-133
[57]张亚丽,徐忠.脱脂豆粕多肽络合亚铁食品添加剂的制备及应用研究.食品工业科技,2004,25(4):120-122
[58]王稳航,刘安军,王丽霞等.酪蛋白酶解多肽-Fe~(2+)的制备及性质研究.食品工业科技,2004,25(8):53-56
[59]邓尚贵,杨燊,秦小明.低值鱼蛋白多肽-铁(Ⅱ)螯合物的酶解制备及其抗氧化、抗菌活性.湛江海洋大学学报,2006,26(4):54-58
[60]刘志伟.补血功能食品[M].北京:化学工业出版社,2005,3
[61]车振明,高平.猪血的综合开发和利用[J].农牧产品开发,2000(3):29-30
[62]冯咏梅,王文华,陈小平.猪血制品的研究与开发[J].食品研究与开发,2002,23(6):71-74
[63]李雨露.猪血的功能特性及开发利用[J].肉类工业,2002(4):25-26
[64]Judit Gál,Andrew Hursthouse,Paul Tatner,et al.Cobalt and secondary poisoning in the terrestrial food chain:Data review and research gaps to support risk assessment.Environment International,In Press,Corrected Proof,Available online,2007,12
[65]Michael Kalafatis,Nancy A.Swords,Matthew D.Rand,et al.Membrane-dependent reactions in blood coagulation:role of the vitamin K-dependent enzyme complexes.Biochimica et Biophysica Acta(BBA) - Molecular Basis of Disease,1994,122 7(3):113-129
[66]J.Rubes,L.Borkovec,Z.Hoíínová,et al.Cytogenetic monitoring of farm animals under conditions of environmental pollution.Mutation Research Letters,1992,283(3):199-210
[67]刘志伟.补血功能食品[M].北京:化学工业出版社,2005,3
[68]褚庆环.动物性食品副产品加工技术[M].青岛:青岛出版社,2005,1
[69]Tanja Goyarts,Nicola Grove,Sven D(a|¨)nicke.Effects of the Fusarium toxin deoxynivalenol from naturally contaminated wheat given subchronicaIly or as one single dose on the in vivo protein synthesis of peripheral blood lymphocytes and plasma proteins in the pig.Food and Chemical Toxicology,2006,44(12):1953-1965
[70]Rajesh Sreenivasan,Preeti G.Joshi,Nanda B.Joshi.Hematoporphyrin derivative induced photodamage to brain tumor cells:Alterations in subcellular membranes.Radiation Physics and Chemistry,1997,49(1):145-149
[71lYing Shan,Jianyu Zheng,Richard W.Lambrecht,et al.Reciprocal Effects of Micro-RNA-122 on Expression of Heme Oxygenase-1 and Hepatitis C Virus Genes in Human Hepatocytes.Gastroenterology,2007,133(4):1166-1174
[72]Robert A.Floyd.Neuroinflammatory processes are important in neurodegenerative diseases:an hypothesis to explain the increased formation of reactive oxygen and nitrogen species as major factors involved in neurodegenerative disease development.Free Radical Biology and Medicine,1999,26(9-10):1346-1355
[73]车志敏,王玉芬,张春晖等.血红蛋白水解液脱除血红素工艺的优化[J].食品工业(上海),2005,26(6):50-52
[74]马美湖.血红蛋白脱色技术及其在食品中的应用[J].农牧产品开发,1996,(10):3-6
[75]王远明.无硝血红蛋白着色剂的研制[J].华东理工大学学报,1998,24(6):655-658
[76]黄群,马美湖,杨抚林.畜禽血液血红蛋白的开发利用[J].肉类工业,2003,(10):19-25
[77]Silvestre M.Review of methods for the analysis of protein hydrolysates[J].Food Chemistry,1997,60(2):263-271
[78]Man-Jin In,Hee Jeong Chae,Nam-Soon Oh.Process development for heine-enriched peptide by enzymatic hydrolysis of hemoglobin[J].Biores.Technol.,2002,84.63-68
[79]杨炜,朋珍荣.高温蛋白酶酶解制备猪血蛋白的研究[J].氨基酸和生物资源,1996,18(2):1-4
[80]张华山.猪血蛋白的酶水解及氨基酸含量[J].氨基酸和生物资源,1999,21(3):35-37
[81]于美娟,马美湖,万佳蓉.复合酶水解猪血液工艺条件的研究[J].肉类工业,2005,(5):14-17
[82]张炜煜 周淡宜.一种提取血红素的新方法研究[J].中国现代应用药学杂志,2000,17(1):35-37
[83]杨严俊,王荣民.猪血中脱色球蛋白的制备及其功能性研究[J].无锡轻工大学学报,1997,16(2):15-20
[84]姜鹭,王寿亭.AAS系列新型吸附树脂对猪血粉水解液脱色性能的研究[J].氨基酸杂志,1989,(1):16-19
[85]马美湖,唐进明.亚硝基蛋白的合成制取及应用于香肠中降低NO_2-残留量的研究[J].食品科学,2001,22(8):67-70
[86]王君,陈红亮,张向东等.氯化血红素提取新工艺的研究[J].当代化工,2001,30(3):125-127
[87]王旭,曲晓波.天然多肽分离与分析方法研究进展[J].长春中医学院学报,2006,22(1):87-88
[88]李艳伟,江波,佟祥山.酶解猪血蛋白中活性肽的纯化和功能研究[J].高等学校化学学报,2005,26(1):61-63)
[89]方俊,卢向阳,蒋红梅.猪血多肽成分及其清除活性氧的能力观察[J].中国临床康复,2006.10(17):122-124
[90]Lantz I,Glamsta E,Talback L,et al.Hemophines derived from hemoglobin have an inhibitory action on angiotensin converting enzyme activity[J].FEBS Lett,1991,287:39
[91]Kazue M,Mikio F,Masayoshi K,et al.Antihypenensive effect of angiontensin I-converting enzyme inhibitory peptides derived from hemoglobin[J].European Journal of Pharmacology,1996,304:93-98
[92]Kyoichi K,Hisako M,Chizuko F.Globin digest,acidic protease hydrolysate,inhibits dietary hypertdglyceridemia and Val-Val-Tyr-Pro,one of its constituents,possesses most superior effect[J].Life Sciences,1996,58(20):1745-1755
[93]Zhao Q,Ganneau I,Sannier F,et al.Opioid peptides derived from hemoglobin:hemorphins [J].Biopoly.1997,43:75-98
[94]余奕珂,胡建恩,白雪芳.珠蛋白水解液生物活性的初步研究[J].食品与药品,2006,8(08A):37-39
[95]钟耀广,南庆贤.血肽的开发利用[J].肉类工业,2002,(3):39-40
[96]Adelia C,Bovell-Benjamin,Lindsay HAlle.Iron absorption from ferrous bisglycinate and ferric trisglycinate in whole maize is regulater by iron status[J].American Society for Clinical Nutrition.2000,71:1563-1569
[97]周桂莲.氨基酸螯合铁的研究与应用进展[J].饲料博览,1998,10(6):10-12
[98]Garcia,Layrisse.The effect of change in pH on the solubility of iron bis-glycinate chelate and other iron compounds[J].Archivos-Latinoamericanos-de-Nutricion.2001,51(1):35-36
[99]Karra.Experience with ferrous bis-glycine chelate as an iron fortificant in milk[J].International Journal for Vitamin and Nutrition Research.2002,72(4):257-263
[100]杨文炳,姚芬英.复合氨基铁合成及其螯合反应研究[J].化学通报,1991(1):46-47
[101]秦卫东,吕兆启,涂宝军.复合氨基酸亚铁的制备研究[J].中国食品添加剂.2003,(6):42-44
[102]林萍,宋常英,张晓鸣.甘氨酸螯合铁的合成工艺[J].无锡轻工大学学报,2004,23(2):53-57
[103]徐海青,明霞.由鸡羽毛制备复合氨基酸铁复合物的方法[J].沈阳农业大学学报.2001,32(1):51-53
[104]陈洁,赵郁,杨红芸等.氨基酸促进硫酸亚铁吸收的药效学研究[J].华西药学杂志.2001,16(6):419-420
[105]周桂莲,韩友文,徐子伟等.不同铁源生物学效价的研究[J].动物营养学报.2002,14(2):47-52
[106]靳雅笙,赖建强,赵显峰.甘氨酸亚铁改善大鼠营养性贫血的效果评价[J].卫生研究,2005,34(3):344-346
[107]Institute of Medicine.Iron.In:Dietary reference intakes for vitamin A,vitamin K,arsenic,boron,chromium,copper,iodine,iron,manganese,molybdenum,nickel,silicon,vanadium,and zinc.Washington,DC:National Academy Press,2002:290-293
[1]钟耀广,南庆贤.国内外畜血研究动态[J].中国农业科技导报,2003,5(3):26-29
[2]李雨露,吕长鑫.猪血的功能特性及开发利用[J].肉品卫生,2001,(12):26-27
[3]Haleem J.Issaq,King C.Chan,George M.Janini.et al.Multidimensional separation of peptides for effective proteomic analysis[J].Journal of Chromatography B,2005,817(1):35-47
[4]郭善广,赵谋明,崔春等.脱色猪血红蛋白酶解产物品质及营养特性研究[J].食品与发酵工业,2007,33(1):10-15
[5]于长青,任泊晓,修峰.鹅血酶解液活性炭脱色效果的研究[J].中国食品添加剂,2007(1):66-69
[6]姜瞻梅,田波,霍贵成.酪蛋白源ACE抑制肽的精制[J].食品工业科技,2007,28(2):187-189
[7]蒋菁莉,任发政,蔡华伟.牛乳酪蛋白降血压肽的超滤分离[J].食品科学,2006,27(7):124-128
[8]G.C.Birch,K.J.Parker.Enzymes and food processing.Applied Science.Publishers Ltd,1981.
[9]肖凯军,高孔荣,曾庆孝.酶解大豆分离蛋白的特性研究[J].广州食品工业科技,1995,11(2):5-10
[10]何照范,张迪清.保健食品化学及其检测技术[M].北京:中国轻工业出版社,1998
[11]周利亘,王君虹,陈新峰等.大豆多肽脱色工艺优化设计[J].食品科技,2004(12):22-25
[12]于美娟,马美湖,万佳蓉.复合酶水解猪血液工艺条件的研究[J].肉类工业,2005(5):14-17
[13]周存山,马海乐,余筱洁等.麦胚蛋白降压肽的大孔树脂脱盐研究[J].食品科学,2006,27(3):142-146
[14]J.T.Clark,L.J.Cutler,G.M.O'Meara.et al.Solubilisation of bovine rumen and decolorisation of bovine blood by enzymic hydrolysis with alcalase.Meat Science,1987,21(2):111-120
[15]Daniel L.Purich.Covalent enzyme-substrate compounds:Detection and catalytic competence.Methods in Enzymology,2002(354):1-27
[16]Stephen R.Sprang,Zhe Chen and Xinlin Du.Structural Basis of Effector Regulation and Signal Termination in Heterotrimeric Gα Proteins.Advances in Protein Chemistry,2007(74):1-65
[17]Jure Stojan,Marko Golicnik and Didier Fournier.Rational polynomial equation as an unbiased approach for the kinetic studies of Drosophila melanogaster acetylcholinesterase reaction mechanism.Biochimica et Biophysica Acta(BBA) - Proteins & Proteomics,2004.1703(1,):53-61
[18]Viadimir I.Krupyanko.A vector method of representation of enzymic reactions:1.Parametric classification of types of enzymic reaction.Process Biochemistry,2004,39(7):805-813
[19]Antonio Carlos Vassalo Alves,Edyr Rogana,Célia de Fátima Barbosa.et al.The correction of reaction rates in continuous fluorometric assays of enzymes.Journal of Biochemical and Biophysical Methods,2007,70(3):471-479
[20]M.C.Márquez and M.A.Vázquez.Modeling of enzymatic protein hydrolysis.Process Biochemistry,1999,35(1-2):111-117
[21]Adrian J.Mulholland and W.Graham Richards.A model of the condensation step in the citrate synthase reaction.Journal of Molecular Structure:THEOCHEM,1998,427(1-3):175-184
[22]Séverine Roué,Claude Lapinte.Iron(Ⅱ) and iron(Ⅲ) complexes containing ethynyl thiophene fragments.Journal of Organometallic Chemistry,2005,690(3):594-604
[23]廖丹葵,万顺刚,黄爱民等.蛋黄降压肽酶解液脱色工艺的优化[J].食品工业科技,2007,28(1):151-153
[24]王运生,李志伟,杨秀.pH值对味精活性炭脱色效果的影响[J].化工生产与技术,2007,14(4):41-43
[25]Sato Y,Hayakawna S,Hayakaea M.Preperation of blood globin through carboxymethyl cellulose chromatography.Food Technology,1981,16:81-89
[26]姚成虎,王志耕,许飞等.活性炭脱除猪血红蛋白酶解液色泽的技术研究[J].食品工业科技,2007,28(9):107-109
[27]刘菊湘,姜根华.用离子交换树脂脱除氨基酸与盐混合液中的盐.离子交换与吸附,2000,16(6):521-527
[28]Nalan Kabay,Sarper Sarp,Mithat Yuksel.et al.Removal of boron from seawater by selective ion exchange resins.Reactive and Functional Polymers,2007,67(12):1643-1650
[29]李云飞,葛克山.食品工程原理[M].中国农业大学出版社,2002,2
[30]Yu-Jin Kim,Sunghoon Park,You-Kwan Oh.et al.Purification and characterization of human easeinomacropeptide produced by a recombinant Saccharomyces cerevisiae.Protein Expression and Purification,2005,41(2):441-446
[1]Oscar Pineda,H.DeWayne Ashmead.Effectiveness of treatment of iron-deficiency anemia in infants and young children with ferrous bis-glycinate chelate.Nutrition,2001,17(5):381-384.
[2]Fernando Pizarro,Raǘl Uicich,Manuel Olivares.et al.Iron absorption of ferric glycinate is controlled by iron stores.Nutrition Research,1998,18(1):3-9
[3]张亚丽,徐忠.脱脂豆粕多肽络合亚铁食品添加剂的制备及应用研究[J].食品工业科技,2004,25(4):120-122
[4]王稳航,刘安军,王丽霞等.酪蛋白酶解多肽-Fe~(2+)的制备及性质研究[J].食品工业科技,2004,25(8):53-56
[5]邓尚贵,杨燊,秦小明.低值鱼蛋白多肽-铁(Ⅱ)螯合物的酶解制备及其抗氧化、抗菌活性[J].湛江海洋大学学报,2006,26(4):54-58
[6]张祥麟.络合物化学[M].北京:冶金工业出版社,1980
[7]中华人民共和国国家标准[S].GB/T 5009.3-2003.
[8]中华人民共和国国家标准[S].GB/5009.5-85
[9]中华人民共和国国家标准[S].GB/T 12457-90
[10]黄伟坤.食品检验与分析[M].北京:中国轻工业出版社,1981
[11]Eugene D.Weinberg.Iron Chelation in Chemotherapy.Advances in Applied Microbiology,2003,52:187-208
[12]Paloma Bermejo,Enrique Pinero,Angel M(?) Villar.Iron-chelating ability and antioxidant properties of phycocyanin isolated from a protean extract of Spirulina platensis.Food Chemistry,2008,110(2):436-445
[13]Joel G.Parkes,Edward W.Randell,Nancy F.Olivieri,et al.Modulation by iron loading and chelation of the uptake of non-transferrin-bound iron by human liver cells.Biochimica et Biophysica Acta(BBA) - General Subjects,1995,1243(3):373-380
[14]王丽霞,刘安军,王稳航.多肽-Fe抗贫血保健功能的研究[J].食品研究与开发,2006(27)7:179-181
[15]徐志固.现代配位化学[M].化学工业出版社,北京:1987,175-183
[1]Breno P.Esposito,Silvina Epsztejn,William Breuer,et al.A Review of Fluorescence Methods for Assessing Labile Iron in Cells and Biological Fluids.Analytical Biochemistry,2002,304(1):1-18
[2]裴夫国,庄红,吕学举等.多肽-铁制备及其性质研究[J].食品科学,2007,28(10):594-596
[3]Nancy C.Andrews.Iron Absorption.Physiology of the Gastrointestinal Tract(Fourth Edition),2006,1983-1992
[4]冯咏梅,王文华.猪血制品的研究与开发[J].食品研究与开发,2002,23(6):71-74
[5]黄伟坤.食品检验与分析[M].北京:中国轻工业出版社,1989,1
[6]张永安.无机化学[M].北京:北京师范大学出版社,2006,6
[7]高素蕴.大豆多肽锌螯合盐的制备及生理活性研究[D].西北农林科技大学硕士学位论文,2003,5
[8]潘思轶.大豆蛋白的分子修饰及特性研究[D].华中农业大学博士学位论文,2005,6
[9]李健,徐忠.复合氨基酸微量元素络合盐的制备工艺研究[J].中国粮油学报,2001,16(3):38-41
[10]钟国清.甘氨酸锌螫合物的合成与结构表征[J].精细化工,2001,18(7):391-393
[11]李小俊,胡克良.红外光谱对碱土金属二元羧酸盐结构的研究[J].光谱学与光谱分析,2002,22(3):392-395
[12]滕冰,韩有文.蛋氨酸螯合锌(Ⅱ)的合成与制备[J].饲料博览,2000(12):2-4
[13]孔薇,李怀洲.大豆饼粕制备复合氨基酸铜螯合物[J].郑州大学学报(医学版),2002.37(5):652-653
[1]张亚丽,徐忠.脱脂豆粕多肽络合亚铁食品添加剂的制备及应用研究[J].食品工业科技,2004,25(4):120-122
[2]刘安军,刘景彬,范艳丽等.多肽-Fe的抗氧化研究[J].食品科技,2006(5):136-138
[3]邓尚贵,杨粲,秦小明.低值鱼蛋白多肽-铁(Ⅱ)螯合物的酶解制备及其抗氧化、抗菌活性[J].湛江海洋大学学报,2006,26(4):54-58
[4]王丽霞,刘安军,王稳航.多肽-Fe抗贫血保健功能的研究[J].食品研究与开发,2006,27(7):179-181
[5]Horwitz W.Official methods of analysis of the association of official analytical chemists[J].14th ed.Washington:Association of Official Analytic chemists,1984:880-882
[6]郭艳华,胡思前.荸荠皮提取物的抗氧化活性研究[J].食品与发酵工业,2007,33(10):128-130
[7]焦中高,刘杰超,王思新.黑莓红色素抗氧化活性的研究[J].食品科技,2003(8):63-65
[8]毕和平,张立伟,韩长日等.三叉苦提取物抗氧化作用的研究[J].食品科学,2007,28(7):57-60
[9]龙盛京,罗佩卓,覃日昌.17种清热中药抗活性氧作用的研究[J].中草药,1999,30(1):40-43
[10]朴晋华,张丽,高天红等.生血素抗贫血的实验研究[J].中国中药杂志.2003,28(6):544-547
[11]杨祖英.食品检验[M].北京:化学工业出版社,2004,7
[12]赵保路.氧自由基和天然抗氧化剂[M]北京:科学出版社,1999,7
[13]Tze-Kiong Er,Shih-Meng Tsai,Szu-Hsien Wu,et al.Antioxidant status and superoxide anion radical generation in acute myeloid leukemia.Clinical Biochemistry,2007,40(13-14):1015-1019
[14]Rosario Jiménez,Rocío Lopez-Sepǘlveda,María Kadmiri,et al.Polyphenols restore endothelial function in DOCA-salt hypertension:Role of endothelin-1 and NADPH oxidase.Free Radical Biology and Medicine,2007,43(3):462-47
[15]徐中儒.回归分析与试验设计[M].北京:中国农业出版社,1997,3
[16]Mukesh Kumar Agarwal,Mohammad Iqbal,Mohammad Athar.Garlic oil ameliorates ferric nitrilotriacetate(Fe-NTA)-induced damage and tumor promotion:Implications for cancer prevention.Food and Chemical Toxicology,2007,45(9):1634-1640
[17]Shazib Pervaiz,Marie-Veronique Clement.Superoxide anion:Oncogenic reactive oxygen species? The International Journal of Biochemistry & Cell Biology,2007,39(7-8):1297-1304
[18]Roger A.Sheldon,Isabel W.C.E.Arends.Catalytic oxidations mediated by metal ions and nitroxyl radicals.Journal of Molecular Catalysis A:Chemical,2006,251(1-2):200-214
[19]Francesco Minisci,Francesco Recupero,Gian Franco Pedulli,et al.Transition metal salts catalysis in the aerobic oxidation of organic compounds:Thermochemical and kinetic aspects and new synthetic developments in the presence of N-hydroxy-derivative catalysts.Journal of Molecular Catalysis A:Chemical,2003,204-205:63-90
[20]郝庆红,陈冠华,冯雅琪等.厚朴中清除氧自由基主要有效成分的确定[J].河北大学学报(自然科学版),2005,25(3):164-166
[21]Gow- Chin Yen,Pin- Der Duh,Da- Yon Chung.Antioxidant activity of anthraquinones and anthrone[J].Food Chemistry,2002(70):437-441
[22]曹洪涛,王菊莉,任春清.缺铁性贫血的临床表现[J].中国社区医师,2004,20(3):13
[23]Hillary J.Dowling,Esther G.Offenbacher,F.Xavier Pi-Sunyer.Effects of amino acids on the absorption of trivalent chromium and its retention by regions of the rat small intestine.Nutrition Research,1990,10(11):1261-1271
[24]Trevor G.Smart,Xinmin Xie,Belinda J.Krishek.Modulation of inhibitory and excitatory amino acid receptor ion channels by zinc.Progress in Neurobiology,1994,42(3):393-441
[1]国家卫生部.GB15193-1994.食品安全性毒理学评价程序和方法[S].
[2]王向东.食品毒理学[M].南京:东南大学出版社,2007,9
[3]宿迷菊,王岳飞,骆耀平等.绿茶口含片安全性毒理学研究[J].茶叶科学,2006,26(2):122-127
[4]张卫明,顾龚平,史劲松.银杏叶食用安全性的毒理学评价.[J].茶叶科学,2005,28(1):80-83
[5]L.J.Thomson,A.A.Hoffmann.Field validation of laboratory-derived IOBC toxicity ratings for natural enemies in commercial vineyards.Biological Control,2006,39(3):507-515
[6]I.Vistad,M.Cvancarova,S.D.Fossa,et al.5027 POSTER Physician-assessed toxicity rating versus patient self-reporting in cervical cancer survivors treated with radiotherapy at Rikshospitalet-Radiumhospitalet Medical Center(RRMC).European Journal of Cancer Supplements,2007,5(4):319
[7]Nget-Hong Tan,Gnanajothy Ponnudurai.A comparative study of the biological properties of krait(genus Bungarus) venoms.Comparative Biochemistry and Physiology Part C:Comparative Pharmacology,1990,95(1):105-109
[8]Torgny N.Fredrickson,Joan M.G.Sechler,Gregory J.Palumbo,et al.Acute inflammatory response to cowpox virus infection of the chorioallantoic membrane of the chick embryo.Virology,1992,187(2):693-704
[9]James T.Macgregor,Mendel Friedman.Nonmutagenicity of tetrabromophthalic anhydride and tetrabromophthalic acid in the Ames Salmonella/microsome mutagenicity test.Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis,1977,56(1)81-83
[10]李建科.食品毒理学[M].北京:中国计量出版社,2007,2
[2]Ana B Pérez-Expósito,Salvador Villalpando,Juan A Rivera.et al.Ferrous Sulfate Is More Bioavailable among Preschoolers than Other Forms of Iron in a Milk-Based Weaning Food Distributed by PROGRESA,a National Program in Mexico 1,2.The Journal of Nutrition,2005,135(1):64-70
[3]Philip A.Candela.The evolution of aqueous vapor from silicate melts:Effect on oxygen fugacity.Geochimica et Cosmochimica Acta,1986,50(6):1205-1211
[4]Hua-Min Xu,Hong Jiang,Jun Wang.et al.Over-expressed Human Divalent Metal Transporter 1 is involved in Iron Accumulation in MES23.5 Cells.Neurochemistry International,In Press,Accepted Manuscript,2007.11.9
[5]Conrad ME,Umbreit JN,Moore EG.et al.Separate pathways for cellular uptake of ferric and ferrous iron.Am J Physiol,2000,279(4):G767-G774
[6]Marcel E.Conrad,Jay N.Umbreit.Pathways of Iron Absorption.Blood Cells,Molecules,and Diseases,2002,29(3):336-355
[7]Maria N.Garcia,Carlos Martinez-Torres,Irene Leets.et al.Heat treatment on heme iron and iron-containing proteins in meat:Iron absorption in humans from diets containing cooked meat fractions.The Journal of Nutritional Biochemistry,1996,7(1):49-54
[8]Levine DS,Huebers HA,Rubin CE.et al.Blocking action of parenteral desferrioxamine on iron absorption in rodents and men.Gastroenterol,1988,95:1242-1248.
[9]Boni RE,Hueh-Boni RA,Gallbraith RA.et al.Tinmesoporphyrin inhibits heme oxygenase activity and heme iron absorption in the intestine.Pharmacol,1993,47:318-329
[10]Yifan Cheng,Elmar Wolf,Mykol Larvie.et al.Single Particle Reconstructions of the Transferrin-Transferrin Receptor Complex Obtained with Different Specimen Preparation Techniques.Journal of Molecular Biology,2006,355(5):1048-1065
[11]Katerina Chvátalová,Iva Slaninová,Lenka Brezinová.et al.Influence of dietary phenolic acids on redox status of iron:Ferrous iron autoxidation and ferric iron reduction.Food Chemistry,2008,106(2):650-660
[12]S.Juanto,R.S.Schrebler,J.O.Zerbino.et al.The influence of different cations on the eletrochemical and ellipsometric behaviour of iron in alkaline media.Electrochiraica Acta,1991,36(7):1143-1150
[13]Mirjana Andjelkovic,John Van Camp,Bruno De Meulenaer.et al.Iron-chelation properties of phenolic acids bearing catechol and galloyl groups.Food Chemistry,2006,98(1):23-31
[14]Tomas Ganz,Elizabeta Nemeth.Regulation of iron acquisition and iron distribution in mammals.Biochimica et Biophysica Acta(BBA) - Molecular Cell Research,2006,1763(7):690-699
[15]Pauline T.Lieu,Marja Heiskala,Per A.Peterson.The roles of iron in health and disease.Molecular Aspects of Medicine,2001,22(1-2):1-87
[16]Teresa Olczak,Waltena Simpson,Xinyan Liu.et al.Iron and heme utilization in Porphyromonas gingivalis.FEMS Microbiology Reviews,2005,29(1):119-144
[17]Dachun Yao,Weibin Shi,Yulan Gou.et al.Fatty acid-mediated intracellular iron translocation:A synergistic mechanism of oxidative injury.Free Radical Biology and Medicine,2005,39(10):1385-1398
[18]Mikio Nakamura,Yoshiki Ohgo,Akira Ikezaki.Electronic ground states of low-spin iron(Ⅲ)porphyrinoids.Journal of Inorganic Biochemistry,In Press,Accepted Manuscript,2007
[19]Jana Gerber,Roland Lill.Biogenesis of iron-sulfur proteins in eukaryotes:components,mechanism and pathology.Mitochondrion,2002,2(1):71-86.
[20]Heiko B(o|¨)nisch,Christian L.Schmidt.et al.The Structure of the Soluble Domain of an Archaeal Rieske Iron-Sulfur Protein at 1.1A Resolution.Journal of Molecular Biology,2002,319(3):791-805
[21]中国营养学会.中国居民膳食营养素参考摄入量(Chinese DRIs).北京:中国轻工业出版社,2000
[22]Craig S.Broberg,Bridget E.Bax,Darlington O.Okonko.et al.Blood Viscosity and its Relationship to Iron Deficiency,Symptoms,and Exercise Capacity in Adults With Cyanotic Congenital Heart Disease.Journal of the American College of Cardiology,2006,48(2):356-365
[23]John W.Eaton,Mingwei Qian.Molecular bases of cellular iron toxicity.Free Radical Biology and Medicine,2002,32(9):833-840
[24]Yuhta Shiono,Shinya Wakusawa,Hisao Hayashi.et al.Iron accumulation in the liver of male patients with Wilson's disease.The American Journal of Gastroenterology,2001,96(11):3147-3151
[25]Srdjan Denic,Mukesh M.Agarwal.Nutritional iron deficiency:an evolutionary perspective.Nutrition,2007,23(7-8):603-614
[26]Philip Lanzkowsky.Iron-deficiency anemia.Manual of Pediatric Hematology and Oncology(Fourth Edition),2005
[27]C.Poitou Bernert,C.Ciangura,M.Coupaye.et al.Nutritional deficiency after gastric bypass:diagnosis,prevention and treatment.Diabetes & Metabolism,2007,33(1):13-24
[28]Yuan-Ji Pan,George Loo.Effect of copper deficiency on oxidative DNA damage in Jurkat T-lymphoeytes.Free Radical Biology and Medicine,2000,28(5):824-830
[29]Joao Banha,Liliana Marques,Rita Oliveira.et al.Ceruloplasmin expression by human peripheral blood lymphocytes:A new link between immunity and iron metabolism.Free Radical Biology and Medicine,In Press,Corrected Proof,2007
[30]Vivien M.Hodges,Susan Rainey.et al.Pathophysiology of anemia and erythrocytosis.Critical Reviews in Oncology/Hematology,2007,64(2):139-158
[31]Victoria Arija,Griselda Esparó,Joan Fernández-Ballart.et al.Nutritional status and performance in test of verbal and non-verbal intelligence in 6 year old children.Intelligence,2006,34(2):141-149.
[32]Jerome Y.Yager,Dawn S.Hartfield.Neurologic manifestations of iron deficiency in childhood.Pediatric Neurology,2002,27(2):85-92
[33]Jehn-Shan Yeh,Ching-Hsue Cheng.Using hierarchical soft computing method to discriminate microcyte anemia.Expert Systems with Applications,2005,29(3):515-524
[34]Robert T.Brown,Susan M.Mcintosh,Vicki R.et al.Iron status of adolescent female athletes.Journal of Adolescent Health Care,1985,6(5):349-352
[35]Kieran E.Fallon.Screening for haematological and iron-related abnormalities in elite athletes-Analysis of 576 cases.Journal of Science and Medicine in Sport,In Press,Corrected Proof,2007
[36]Kelly W.Mercer,John J.Densmore.Hematologic Disorders in the Athlete.Clinics in Sports Medicine,2005,24(3):599-621
[37]Beard J,Borel M J,Peterson F J.Change in iron status during weight loss with very-low energy diets.Clinic Nutrition,1997,66(1):104-110
[38]Shane R.Cunha,Peter J.Mohler.Cardiac ankyrins:Essential components for development and maintenance of excitable membrane domains in heart.Cardiovascular Research,2006,71(1):22-29
[39]Richard Shingles,Marisa North,Richard E.McCarty.Direct Measurement of Ferrous Ion Transport across Membranes Using a Sensitive Fluorometric Assay.Analytical Biochemistry,2001,296(1):106-113
[40]Maria Kapsokefalou,Vasilis Kakouris,Kyriakos Makris.et al.Oxidative activity and dialyzability of some iron compounds under conditions of a simulated gastrointestinal digestion in the presence of phytate.Food Chemistry,2007,101(1):419-427
[41]Alexandra Kiskini,Konstantina Argiri,Michael Kalogeropoulos.et al.Sensory characteristics and iron dialyzability of gluten-free bread fortified with iron.Food Chemistry,2007,102(1):309-316
[42]Oscar Pineda,H.DeWayne Ashmead.Effectiveness of treatment of iron-deficiency anemia in infants and young children with ferrous bis-glycinate chelate.Nutrition,2001,17(5):381-384
[43]Fernando Pizarro,Raúl Uicich,Manuel Olivares.et al.Iron absorption of ferric glycinate is controlled by iron stores.Nutrition Research,1998,18(1):3-9
[44]Hiroshi Fujii.Electronic structure and reactivity of high-valent oxo iron porphyrins. Coordination Chemistry Reviews,2002,226(1-2):51-60
[45]B.Gulbis,F.Cotton,F.Vertongen.Hémoglobines anormales rares.EMC -Hématologie,2004,1(4):106-114
[46]Tracey A.Rouault.The Intestinal Heme Transporter Revealed.Cell,2005,122(5):649-651
[47]钟才云,马凤楼,蔡凤鸣.血红素铁治疗女大学生缺铁性贫血的研究.中国校医,1995,9(3):167-169
[48]陈苏陵,唐明龙.从猪血中制备血红素铁及血红素铁强化食品纠正缺铁性贫血疗效分析.桂林医学院学报,1994(1):8-9
[49]钟才云,马凤楼.血红素铁的生物利用研究.营养学报,1989,11(2):135-140
[50]刘雅琴,王德颖,王朝辉等.CMC-血红素铁防治小儿缺铁性贫血的临床研究.医师进修杂志,1999(3):21
[51]J.A.Rendleman.Metal-polysaccharide complexes-Part Ⅰ.Food Chemistry,1978,3(1):47-79
[52]Vlasta Mohacek-Grosev,Romano Bozac,Gerwin J.Puppels.Vibrational spectroscopic characterization of wild growing mushrooms and toadstools.Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2001,5 7(14):2815-2829
[53]Rosalee S.Rasmussen,Michael T.Morrissey.Marine Biotechnology for Production of Food Ingredients.Advances in Food and Nutrition Research,2007,52:237-292
[54]孟凡德,赵全芹,李明霞.紫菜多糖Fe(Ⅲ)配合物的生物利用度初步研究.中国现代应用药学杂志,2002,19(3):207-209
[55]李公斌,王振宇.黑木耳多糖与Fe(Ⅱ)螯合物的制备工艺及红外光谱研究.食品工业科技,2006,27(5):136-138
[56]Roberta J.Ward,Martin J.O'Connell,Dominic P.E.Dickson.et al.Biochemical studies of the iron cores and polypeptide shells of haemosiderin isolated from patients with primary or secondary haemochromatosis.Biochimica et Biophysica Acta(BBA) - General Subjects,1989,993,(1):131-133
[57]张亚丽,徐忠.脱脂豆粕多肽络合亚铁食品添加剂的制备及应用研究.食品工业科技,2004,25(4):120-122
[58]王稳航,刘安军,王丽霞等.酪蛋白酶解多肽-Fe~(2+)的制备及性质研究.食品工业科技,2004,25(8):53-56
[59]邓尚贵,杨燊,秦小明.低值鱼蛋白多肽-铁(Ⅱ)螯合物的酶解制备及其抗氧化、抗菌活性.湛江海洋大学学报,2006,26(4):54-58
[60]刘志伟.补血功能食品[M].北京:化学工业出版社,2005,3
[61]车振明,高平.猪血的综合开发和利用[J].农牧产品开发,2000(3):29-30
[62]冯咏梅,王文华,陈小平.猪血制品的研究与开发[J].食品研究与开发,2002,23(6):71-74
[63]李雨露.猪血的功能特性及开发利用[J].肉类工业,2002(4):25-26
[64]Judit Gál,Andrew Hursthouse,Paul Tatner,et al.Cobalt and secondary poisoning in the terrestrial food chain:Data review and research gaps to support risk assessment.Environment International,In Press,Corrected Proof,Available online,2007,12
[65]Michael Kalafatis,Nancy A.Swords,Matthew D.Rand,et al.Membrane-dependent reactions in blood coagulation:role of the vitamin K-dependent enzyme complexes.Biochimica et Biophysica Acta(BBA) - Molecular Basis of Disease,1994,122 7(3):113-129
[66]J.Rubes,L.Borkovec,Z.Hoíínová,et al.Cytogenetic monitoring of farm animals under conditions of environmental pollution.Mutation Research Letters,1992,283(3):199-210
[67]刘志伟.补血功能食品[M].北京:化学工业出版社,2005,3
[68]褚庆环.动物性食品副产品加工技术[M].青岛:青岛出版社,2005,1
[69]Tanja Goyarts,Nicola Grove,Sven D(a|¨)nicke.Effects of the Fusarium toxin deoxynivalenol from naturally contaminated wheat given subchronicaIly or as one single dose on the in vivo protein synthesis of peripheral blood lymphocytes and plasma proteins in the pig.Food and Chemical Toxicology,2006,44(12):1953-1965
[70]Rajesh Sreenivasan,Preeti G.Joshi,Nanda B.Joshi.Hematoporphyrin derivative induced photodamage to brain tumor cells:Alterations in subcellular membranes.Radiation Physics and Chemistry,1997,49(1):145-149
[71lYing Shan,Jianyu Zheng,Richard W.Lambrecht,et al.Reciprocal Effects of Micro-RNA-122 on Expression of Heme Oxygenase-1 and Hepatitis C Virus Genes in Human Hepatocytes.Gastroenterology,2007,133(4):1166-1174
[72]Robert A.Floyd.Neuroinflammatory processes are important in neurodegenerative diseases:an hypothesis to explain the increased formation of reactive oxygen and nitrogen species as major factors involved in neurodegenerative disease development.Free Radical Biology and Medicine,1999,26(9-10):1346-1355
[73]车志敏,王玉芬,张春晖等.血红蛋白水解液脱除血红素工艺的优化[J].食品工业(上海),2005,26(6):50-52
[74]马美湖.血红蛋白脱色技术及其在食品中的应用[J].农牧产品开发,1996,(10):3-6
[75]王远明.无硝血红蛋白着色剂的研制[J].华东理工大学学报,1998,24(6):655-658
[76]黄群,马美湖,杨抚林.畜禽血液血红蛋白的开发利用[J].肉类工业,2003,(10):19-25
[77]Silvestre M.Review of methods for the analysis of protein hydrolysates[J].Food Chemistry,1997,60(2):263-271
[78]Man-Jin In,Hee Jeong Chae,Nam-Soon Oh.Process development for heine-enriched peptide by enzymatic hydrolysis of hemoglobin[J].Biores.Technol.,2002,84.63-68
[79]杨炜,朋珍荣.高温蛋白酶酶解制备猪血蛋白的研究[J].氨基酸和生物资源,1996,18(2):1-4
[80]张华山.猪血蛋白的酶水解及氨基酸含量[J].氨基酸和生物资源,1999,21(3):35-37
[81]于美娟,马美湖,万佳蓉.复合酶水解猪血液工艺条件的研究[J].肉类工业,2005,(5):14-17
[82]张炜煜 周淡宜.一种提取血红素的新方法研究[J].中国现代应用药学杂志,2000,17(1):35-37
[83]杨严俊,王荣民.猪血中脱色球蛋白的制备及其功能性研究[J].无锡轻工大学学报,1997,16(2):15-20
[84]姜鹭,王寿亭.AAS系列新型吸附树脂对猪血粉水解液脱色性能的研究[J].氨基酸杂志,1989,(1):16-19
[85]马美湖,唐进明.亚硝基蛋白的合成制取及应用于香肠中降低NO_2-残留量的研究[J].食品科学,2001,22(8):67-70
[86]王君,陈红亮,张向东等.氯化血红素提取新工艺的研究[J].当代化工,2001,30(3):125-127
[87]王旭,曲晓波.天然多肽分离与分析方法研究进展[J].长春中医学院学报,2006,22(1):87-88
[88]李艳伟,江波,佟祥山.酶解猪血蛋白中活性肽的纯化和功能研究[J].高等学校化学学报,2005,26(1):61-63)
[89]方俊,卢向阳,蒋红梅.猪血多肽成分及其清除活性氧的能力观察[J].中国临床康复,2006.10(17):122-124
[90]Lantz I,Glamsta E,Talback L,et al.Hemophines derived from hemoglobin have an inhibitory action on angiotensin converting enzyme activity[J].FEBS Lett,1991,287:39
[91]Kazue M,Mikio F,Masayoshi K,et al.Antihypenensive effect of angiontensin I-converting enzyme inhibitory peptides derived from hemoglobin[J].European Journal of Pharmacology,1996,304:93-98
[92]Kyoichi K,Hisako M,Chizuko F.Globin digest,acidic protease hydrolysate,inhibits dietary hypertdglyceridemia and Val-Val-Tyr-Pro,one of its constituents,possesses most superior effect[J].Life Sciences,1996,58(20):1745-1755
[93]Zhao Q,Ganneau I,Sannier F,et al.Opioid peptides derived from hemoglobin:hemorphins [J].Biopoly.1997,43:75-98
[94]余奕珂,胡建恩,白雪芳.珠蛋白水解液生物活性的初步研究[J].食品与药品,2006,8(08A):37-39
[95]钟耀广,南庆贤.血肽的开发利用[J].肉类工业,2002,(3):39-40
[96]Adelia C,Bovell-Benjamin,Lindsay HAlle.Iron absorption from ferrous bisglycinate and ferric trisglycinate in whole maize is regulater by iron status[J].American Society for Clinical Nutrition.2000,71:1563-1569
[97]周桂莲.氨基酸螯合铁的研究与应用进展[J].饲料博览,1998,10(6):10-12
[98]Garcia,Layrisse.The effect of change in pH on the solubility of iron bis-glycinate chelate and other iron compounds[J].Archivos-Latinoamericanos-de-Nutricion.2001,51(1):35-36
[99]Karra.Experience with ferrous bis-glycine chelate as an iron fortificant in milk[J].International Journal for Vitamin and Nutrition Research.2002,72(4):257-263
[100]杨文炳,姚芬英.复合氨基铁合成及其螯合反应研究[J].化学通报,1991(1):46-47
[101]秦卫东,吕兆启,涂宝军.复合氨基酸亚铁的制备研究[J].中国食品添加剂.2003,(6):42-44
[102]林萍,宋常英,张晓鸣.甘氨酸螯合铁的合成工艺[J].无锡轻工大学学报,2004,23(2):53-57
[103]徐海青,明霞.由鸡羽毛制备复合氨基酸铁复合物的方法[J].沈阳农业大学学报.2001,32(1):51-53
[104]陈洁,赵郁,杨红芸等.氨基酸促进硫酸亚铁吸收的药效学研究[J].华西药学杂志.2001,16(6):419-420
[105]周桂莲,韩友文,徐子伟等.不同铁源生物学效价的研究[J].动物营养学报.2002,14(2):47-52
[106]靳雅笙,赖建强,赵显峰.甘氨酸亚铁改善大鼠营养性贫血的效果评价[J].卫生研究,2005,34(3):344-346
[107]Institute of Medicine.Iron.In:Dietary reference intakes for vitamin A,vitamin K,arsenic,boron,chromium,copper,iodine,iron,manganese,molybdenum,nickel,silicon,vanadium,and zinc.Washington,DC:National Academy Press,2002:290-293
[1]钟耀广,南庆贤.国内外畜血研究动态[J].中国农业科技导报,2003,5(3):26-29
[2]李雨露,吕长鑫.猪血的功能特性及开发利用[J].肉品卫生,2001,(12):26-27
[3]Haleem J.Issaq,King C.Chan,George M.Janini.et al.Multidimensional separation of peptides for effective proteomic analysis[J].Journal of Chromatography B,2005,817(1):35-47
[4]郭善广,赵谋明,崔春等.脱色猪血红蛋白酶解产物品质及营养特性研究[J].食品与发酵工业,2007,33(1):10-15
[5]于长青,任泊晓,修峰.鹅血酶解液活性炭脱色效果的研究[J].中国食品添加剂,2007(1):66-69
[6]姜瞻梅,田波,霍贵成.酪蛋白源ACE抑制肽的精制[J].食品工业科技,2007,28(2):187-189
[7]蒋菁莉,任发政,蔡华伟.牛乳酪蛋白降血压肽的超滤分离[J].食品科学,2006,27(7):124-128
[8]G.C.Birch,K.J.Parker.Enzymes and food processing.Applied Science.Publishers Ltd,1981.
[9]肖凯军,高孔荣,曾庆孝.酶解大豆分离蛋白的特性研究[J].广州食品工业科技,1995,11(2):5-10
[10]何照范,张迪清.保健食品化学及其检测技术[M].北京:中国轻工业出版社,1998
[11]周利亘,王君虹,陈新峰等.大豆多肽脱色工艺优化设计[J].食品科技,2004(12):22-25
[12]于美娟,马美湖,万佳蓉.复合酶水解猪血液工艺条件的研究[J].肉类工业,2005(5):14-17
[13]周存山,马海乐,余筱洁等.麦胚蛋白降压肽的大孔树脂脱盐研究[J].食品科学,2006,27(3):142-146
[14]J.T.Clark,L.J.Cutler,G.M.O'Meara.et al.Solubilisation of bovine rumen and decolorisation of bovine blood by enzymic hydrolysis with alcalase.Meat Science,1987,21(2):111-120
[15]Daniel L.Purich.Covalent enzyme-substrate compounds:Detection and catalytic competence.Methods in Enzymology,2002(354):1-27
[16]Stephen R.Sprang,Zhe Chen and Xinlin Du.Structural Basis of Effector Regulation and Signal Termination in Heterotrimeric Gα Proteins.Advances in Protein Chemistry,2007(74):1-65
[17]Jure Stojan,Marko Golicnik and Didier Fournier.Rational polynomial equation as an unbiased approach for the kinetic studies of Drosophila melanogaster acetylcholinesterase reaction mechanism.Biochimica et Biophysica Acta(BBA) - Proteins & Proteomics,2004.1703(1,):53-61
[18]Viadimir I.Krupyanko.A vector method of representation of enzymic reactions:1.Parametric classification of types of enzymic reaction.Process Biochemistry,2004,39(7):805-813
[19]Antonio Carlos Vassalo Alves,Edyr Rogana,Célia de Fátima Barbosa.et al.The correction of reaction rates in continuous fluorometric assays of enzymes.Journal of Biochemical and Biophysical Methods,2007,70(3):471-479
[20]M.C.Márquez and M.A.Vázquez.Modeling of enzymatic protein hydrolysis.Process Biochemistry,1999,35(1-2):111-117
[21]Adrian J.Mulholland and W.Graham Richards.A model of the condensation step in the citrate synthase reaction.Journal of Molecular Structure:THEOCHEM,1998,427(1-3):175-184
[22]Séverine Roué,Claude Lapinte.Iron(Ⅱ) and iron(Ⅲ) complexes containing ethynyl thiophene fragments.Journal of Organometallic Chemistry,2005,690(3):594-604
[23]廖丹葵,万顺刚,黄爱民等.蛋黄降压肽酶解液脱色工艺的优化[J].食品工业科技,2007,28(1):151-153
[24]王运生,李志伟,杨秀.pH值对味精活性炭脱色效果的影响[J].化工生产与技术,2007,14(4):41-43
[25]Sato Y,Hayakawna S,Hayakaea M.Preperation of blood globin through carboxymethyl cellulose chromatography.Food Technology,1981,16:81-89
[26]姚成虎,王志耕,许飞等.活性炭脱除猪血红蛋白酶解液色泽的技术研究[J].食品工业科技,2007,28(9):107-109
[27]刘菊湘,姜根华.用离子交换树脂脱除氨基酸与盐混合液中的盐.离子交换与吸附,2000,16(6):521-527
[28]Nalan Kabay,Sarper Sarp,Mithat Yuksel.et al.Removal of boron from seawater by selective ion exchange resins.Reactive and Functional Polymers,2007,67(12):1643-1650
[29]李云飞,葛克山.食品工程原理[M].中国农业大学出版社,2002,2
[30]Yu-Jin Kim,Sunghoon Park,You-Kwan Oh.et al.Purification and characterization of human easeinomacropeptide produced by a recombinant Saccharomyces cerevisiae.Protein Expression and Purification,2005,41(2):441-446
[1]Oscar Pineda,H.DeWayne Ashmead.Effectiveness of treatment of iron-deficiency anemia in infants and young children with ferrous bis-glycinate chelate.Nutrition,2001,17(5):381-384.
[2]Fernando Pizarro,Raǘl Uicich,Manuel Olivares.et al.Iron absorption of ferric glycinate is controlled by iron stores.Nutrition Research,1998,18(1):3-9
[3]张亚丽,徐忠.脱脂豆粕多肽络合亚铁食品添加剂的制备及应用研究[J].食品工业科技,2004,25(4):120-122
[4]王稳航,刘安军,王丽霞等.酪蛋白酶解多肽-Fe~(2+)的制备及性质研究[J].食品工业科技,2004,25(8):53-56
[5]邓尚贵,杨燊,秦小明.低值鱼蛋白多肽-铁(Ⅱ)螯合物的酶解制备及其抗氧化、抗菌活性[J].湛江海洋大学学报,2006,26(4):54-58
[6]张祥麟.络合物化学[M].北京:冶金工业出版社,1980
[7]中华人民共和国国家标准[S].GB/T 5009.3-2003.
[8]中华人民共和国国家标准[S].GB/5009.5-85
[9]中华人民共和国国家标准[S].GB/T 12457-90
[10]黄伟坤.食品检验与分析[M].北京:中国轻工业出版社,1981
[11]Eugene D.Weinberg.Iron Chelation in Chemotherapy.Advances in Applied Microbiology,2003,52:187-208
[12]Paloma Bermejo,Enrique Pinero,Angel M(?) Villar.Iron-chelating ability and antioxidant properties of phycocyanin isolated from a protean extract of Spirulina platensis.Food Chemistry,2008,110(2):436-445
[13]Joel G.Parkes,Edward W.Randell,Nancy F.Olivieri,et al.Modulation by iron loading and chelation of the uptake of non-transferrin-bound iron by human liver cells.Biochimica et Biophysica Acta(BBA) - General Subjects,1995,1243(3):373-380
[14]王丽霞,刘安军,王稳航.多肽-Fe抗贫血保健功能的研究[J].食品研究与开发,2006(27)7:179-181
[15]徐志固.现代配位化学[M].化学工业出版社,北京:1987,175-183
[1]Breno P.Esposito,Silvina Epsztejn,William Breuer,et al.A Review of Fluorescence Methods for Assessing Labile Iron in Cells and Biological Fluids.Analytical Biochemistry,2002,304(1):1-18
[2]裴夫国,庄红,吕学举等.多肽-铁制备及其性质研究[J].食品科学,2007,28(10):594-596
[3]Nancy C.Andrews.Iron Absorption.Physiology of the Gastrointestinal Tract(Fourth Edition),2006,1983-1992
[4]冯咏梅,王文华.猪血制品的研究与开发[J].食品研究与开发,2002,23(6):71-74
[5]黄伟坤.食品检验与分析[M].北京:中国轻工业出版社,1989,1
[6]张永安.无机化学[M].北京:北京师范大学出版社,2006,6
[7]高素蕴.大豆多肽锌螯合盐的制备及生理活性研究[D].西北农林科技大学硕士学位论文,2003,5
[8]潘思轶.大豆蛋白的分子修饰及特性研究[D].华中农业大学博士学位论文,2005,6
[9]李健,徐忠.复合氨基酸微量元素络合盐的制备工艺研究[J].中国粮油学报,2001,16(3):38-41
[10]钟国清.甘氨酸锌螫合物的合成与结构表征[J].精细化工,2001,18(7):391-393
[11]李小俊,胡克良.红外光谱对碱土金属二元羧酸盐结构的研究[J].光谱学与光谱分析,2002,22(3):392-395
[12]滕冰,韩有文.蛋氨酸螯合锌(Ⅱ)的合成与制备[J].饲料博览,2000(12):2-4
[13]孔薇,李怀洲.大豆饼粕制备复合氨基酸铜螯合物[J].郑州大学学报(医学版),2002.37(5):652-653
[1]张亚丽,徐忠.脱脂豆粕多肽络合亚铁食品添加剂的制备及应用研究[J].食品工业科技,2004,25(4):120-122
[2]刘安军,刘景彬,范艳丽等.多肽-Fe的抗氧化研究[J].食品科技,2006(5):136-138
[3]邓尚贵,杨粲,秦小明.低值鱼蛋白多肽-铁(Ⅱ)螯合物的酶解制备及其抗氧化、抗菌活性[J].湛江海洋大学学报,2006,26(4):54-58
[4]王丽霞,刘安军,王稳航.多肽-Fe抗贫血保健功能的研究[J].食品研究与开发,2006,27(7):179-181
[5]Horwitz W.Official methods of analysis of the association of official analytical chemists[J].14th ed.Washington:Association of Official Analytic chemists,1984:880-882
[6]郭艳华,胡思前.荸荠皮提取物的抗氧化活性研究[J].食品与发酵工业,2007,33(10):128-130
[7]焦中高,刘杰超,王思新.黑莓红色素抗氧化活性的研究[J].食品科技,2003(8):63-65
[8]毕和平,张立伟,韩长日等.三叉苦提取物抗氧化作用的研究[J].食品科学,2007,28(7):57-60
[9]龙盛京,罗佩卓,覃日昌.17种清热中药抗活性氧作用的研究[J].中草药,1999,30(1):40-43
[10]朴晋华,张丽,高天红等.生血素抗贫血的实验研究[J].中国中药杂志.2003,28(6):544-547
[11]杨祖英.食品检验[M].北京:化学工业出版社,2004,7
[12]赵保路.氧自由基和天然抗氧化剂[M]北京:科学出版社,1999,7
[13]Tze-Kiong Er,Shih-Meng Tsai,Szu-Hsien Wu,et al.Antioxidant status and superoxide anion radical generation in acute myeloid leukemia.Clinical Biochemistry,2007,40(13-14):1015-1019
[14]Rosario Jiménez,Rocío Lopez-Sepǘlveda,María Kadmiri,et al.Polyphenols restore endothelial function in DOCA-salt hypertension:Role of endothelin-1 and NADPH oxidase.Free Radical Biology and Medicine,2007,43(3):462-47
[15]徐中儒.回归分析与试验设计[M].北京:中国农业出版社,1997,3
[16]Mukesh Kumar Agarwal,Mohammad Iqbal,Mohammad Athar.Garlic oil ameliorates ferric nitrilotriacetate(Fe-NTA)-induced damage and tumor promotion:Implications for cancer prevention.Food and Chemical Toxicology,2007,45(9):1634-1640
[17]Shazib Pervaiz,Marie-Veronique Clement.Superoxide anion:Oncogenic reactive oxygen species? The International Journal of Biochemistry & Cell Biology,2007,39(7-8):1297-1304
[18]Roger A.Sheldon,Isabel W.C.E.Arends.Catalytic oxidations mediated by metal ions and nitroxyl radicals.Journal of Molecular Catalysis A:Chemical,2006,251(1-2):200-214
[19]Francesco Minisci,Francesco Recupero,Gian Franco Pedulli,et al.Transition metal salts catalysis in the aerobic oxidation of organic compounds:Thermochemical and kinetic aspects and new synthetic developments in the presence of N-hydroxy-derivative catalysts.Journal of Molecular Catalysis A:Chemical,2003,204-205:63-90
[20]郝庆红,陈冠华,冯雅琪等.厚朴中清除氧自由基主要有效成分的确定[J].河北大学学报(自然科学版),2005,25(3):164-166
[21]Gow- Chin Yen,Pin- Der Duh,Da- Yon Chung.Antioxidant activity of anthraquinones and anthrone[J].Food Chemistry,2002(70):437-441
[22]曹洪涛,王菊莉,任春清.缺铁性贫血的临床表现[J].中国社区医师,2004,20(3):13
[23]Hillary J.Dowling,Esther G.Offenbacher,F.Xavier Pi-Sunyer.Effects of amino acids on the absorption of trivalent chromium and its retention by regions of the rat small intestine.Nutrition Research,1990,10(11):1261-1271
[24]Trevor G.Smart,Xinmin Xie,Belinda J.Krishek.Modulation of inhibitory and excitatory amino acid receptor ion channels by zinc.Progress in Neurobiology,1994,42(3):393-441
[1]国家卫生部.GB15193-1994.食品安全性毒理学评价程序和方法[S].
[2]王向东.食品毒理学[M].南京:东南大学出版社,2007,9
[3]宿迷菊,王岳飞,骆耀平等.绿茶口含片安全性毒理学研究[J].茶叶科学,2006,26(2):122-127
[4]张卫明,顾龚平,史劲松.银杏叶食用安全性的毒理学评价.[J].茶叶科学,2005,28(1):80-83
[5]L.J.Thomson,A.A.Hoffmann.Field validation of laboratory-derived IOBC toxicity ratings for natural enemies in commercial vineyards.Biological Control,2006,39(3):507-515
[6]I.Vistad,M.Cvancarova,S.D.Fossa,et al.5027 POSTER Physician-assessed toxicity rating versus patient self-reporting in cervical cancer survivors treated with radiotherapy at Rikshospitalet-Radiumhospitalet Medical Center(RRMC).European Journal of Cancer Supplements,2007,5(4):319
[7]Nget-Hong Tan,Gnanajothy Ponnudurai.A comparative study of the biological properties of krait(genus Bungarus) venoms.Comparative Biochemistry and Physiology Part C:Comparative Pharmacology,1990,95(1):105-109
[8]Torgny N.Fredrickson,Joan M.G.Sechler,Gregory J.Palumbo,et al.Acute inflammatory response to cowpox virus infection of the chorioallantoic membrane of the chick embryo.Virology,1992,187(2):693-704
[9]James T.Macgregor,Mendel Friedman.Nonmutagenicity of tetrabromophthalic anhydride and tetrabromophthalic acid in the Ames Salmonella/microsome mutagenicity test.Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis,1977,56(1)81-83
[10]李建科.食品毒理学[M].北京:中国计量出版社,2007,2