三种常见心血管疾病患者红细胞的光镊拉曼光谱研究
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摘要
目的:利用激光光镊拉曼光谱系统,测定健康者红细胞、脐带血红细胞与三种心血管疾病患者红细胞的拉曼光谱,研究其拉曼光谱之间是否存在差异,探讨健康者红细胞、脐带血红细胞和心血管疾病患者红细胞的内容物的改变情况及其变化机理,为疾病的诊断提供有价值的信息。
方法:取健康献血者、胎儿娩出胎盘未剥的脐带血和三种心血管疾病(先天性心脏病、心律失常、心肌梗死)患者新鲜静脉血标本,以上各标本经洗涤、离心处理后分离出红细胞,用激光光镊拉曼系统采集其红细胞的拉曼光谱。
结果:1.脐带血红细胞的拉曼光谱与健康者红细胞的光镊拉曼光谱差异不明显。2.脐带血红细胞与先天性心脏病患儿红细胞的光镊拉曼光谱有较大的差异;与脐带血红细胞相比,先天性心脏病患儿红细胞的整体谱线偏弱;部分的特征谱数发生位移,例如谱数1273 cm-1移到了1264 cm-1;而谱数1546 cm-1强度降低明显。3.健康者的红细胞与其它心血管疾病患者红细胞的光镊拉曼光谱:(1)健康者红细胞和心律失常患者红细胞比较:①心律失常患者红细胞的部分谱线整体强度有所减弱;②谱数1453 cm-1移至1449 cm-1;③谱数863 cm-1强度变弱,峰形变宽;谱数1212 cm-1和谱数1226 cm-1两峰合为谱数1216 cm-1。另外,低强度He-Ne激光对心律失常患者红细胞有一定的影响。(2)健康者红细胞和心肌梗死患者红细胞比较:①两谱线整体强度变化不大。②谱数1273 cm-1强度明显降低。③谱数1078 cm-1移至1089 cm-1。
结论:(1)利用光镊拉曼光谱可测定和分析脐带血红细胞与先天性心脏病患儿红细胞之间的差异,为先天性心脏病的产前诊断提供有用信息,这将可能为提高胎儿先天性心脏病的检出率提供一种新的、快速简便的光谱分析手段。(2)通过对健康者红细胞与三种常见心血管疾病患者红细胞的拉曼光谱比较,根据拉曼光谱差异性,了解红细胞在不同类型心血管疾病的作用下细胞中分子结构、构象、组成的变化,可以进一步研究常见心血管疾病的拉曼光谱模型,从分子水平探讨心血管疾病的类型及发病机制,为临床诊断提供有力的实验依据。
Objective: Making use of optical tweezers Raman spectroscopy system to study whether there are differences among normal human red blood cells, umbilical cord blood RBC and the RBC in patients with common heart disease and to investigate the changes of the contents in RBC and its variation mechanism, so as to provide valuable information for the diagnosis of disease. Methods: Taking the fresh venous blood samples from healthy blood donors, patients with common heart disease and umbilical cord blood of fetal which is delivered but the placenta is not peeled, and then isolating RBC after washed and centrifuged, and determining Raman spectra of RBC by optical tweezers Raman spectroscopy system.
Results:1. The differences of RBC Raman spectra between umbilical cord blood and normal human are not obvious. 2. Compared with umbilical cord blood RBC, the overall intensity of Raman spectra in patients with congenital heart disease is significantly different. The overall spectrum of RBC in patients with congenital heart disease is weaker then umbilical cord blood RBC. Some characteristic peaks have a few displacements. Spectrum of 1273 cm-1 moved to 1264 cm-1; Intensity of spectrum of 1546 cm-1 reduced significantly. 3. RBC Raman spectra of normal human and patients with other heart disease :(1) Comparison between normal humans’RBC and RBC in patients with arrhythmias:①Some of the overall intensity from patients with arrhythmias is also weak.②The spectrum of 1453 cm-1 moved to 1449 cm-1.③The intensity of spectral of 863 cm-1 weakens and peak deformation widens the peaks of spectrum 1212 cm-1 and 1226 cm-1 combine into spectrum 1216 cm-1. In addition, the low intensity He-Ne laser irradiation on RBC in patients with arrhythmias has a certain impact. (2) Comparison the RBC between normal humans’and patients with myocardial infarction:①There was only little change in the overall intensity of two spectrums.②The intensity of spectrum 1273 cm-1 reduced significantly.③The spectrum of 1078 cm-1 moved to 1089 cm-1.
Conclusion:(1) Optical tweezers Raman spectroscopy can determine and analysis the difference of RBC between umbilical cord blood and patients with congenital heart disease and provide useful information for prenatal diagnosis, which may provide a new, quick and easy methods of spectral analysis for improving the detection rate of fetal congenital heart disease. (2) By comparing the Raman spectra of normal RBC and three kinds of RBC in patients with heart disease, we can understand the changes of RBC molecular structure, conformation and composition in different types of cardiovascular disease, and further study Raman spectra model of cardiovascular disease. We can also investigate the type and pathogenesis of cardiovascular disease from the molecular level and provide reliable experimental evidence for clinical diagnosis.
方法:取健康献血者、胎儿娩出胎盘未剥的脐带血和三种心血管疾病(先天性心脏病、心律失常、心肌梗死)患者新鲜静脉血标本,以上各标本经洗涤、离心处理后分离出红细胞,用激光光镊拉曼系统采集其红细胞的拉曼光谱。
结果:1.脐带血红细胞的拉曼光谱与健康者红细胞的光镊拉曼光谱差异不明显。2.脐带血红细胞与先天性心脏病患儿红细胞的光镊拉曼光谱有较大的差异;与脐带血红细胞相比,先天性心脏病患儿红细胞的整体谱线偏弱;部分的特征谱数发生位移,例如谱数1273 cm-1移到了1264 cm-1;而谱数1546 cm-1强度降低明显。3.健康者的红细胞与其它心血管疾病患者红细胞的光镊拉曼光谱:(1)健康者红细胞和心律失常患者红细胞比较:①心律失常患者红细胞的部分谱线整体强度有所减弱;②谱数1453 cm-1移至1449 cm-1;③谱数863 cm-1强度变弱,峰形变宽;谱数1212 cm-1和谱数1226 cm-1两峰合为谱数1216 cm-1。另外,低强度He-Ne激光对心律失常患者红细胞有一定的影响。(2)健康者红细胞和心肌梗死患者红细胞比较:①两谱线整体强度变化不大。②谱数1273 cm-1强度明显降低。③谱数1078 cm-1移至1089 cm-1。
结论:(1)利用光镊拉曼光谱可测定和分析脐带血红细胞与先天性心脏病患儿红细胞之间的差异,为先天性心脏病的产前诊断提供有用信息,这将可能为提高胎儿先天性心脏病的检出率提供一种新的、快速简便的光谱分析手段。(2)通过对健康者红细胞与三种常见心血管疾病患者红细胞的拉曼光谱比较,根据拉曼光谱差异性,了解红细胞在不同类型心血管疾病的作用下细胞中分子结构、构象、组成的变化,可以进一步研究常见心血管疾病的拉曼光谱模型,从分子水平探讨心血管疾病的类型及发病机制,为临床诊断提供有力的实验依据。
Objective: Making use of optical tweezers Raman spectroscopy system to study whether there are differences among normal human red blood cells, umbilical cord blood RBC and the RBC in patients with common heart disease and to investigate the changes of the contents in RBC and its variation mechanism, so as to provide valuable information for the diagnosis of disease. Methods: Taking the fresh venous blood samples from healthy blood donors, patients with common heart disease and umbilical cord blood of fetal which is delivered but the placenta is not peeled, and then isolating RBC after washed and centrifuged, and determining Raman spectra of RBC by optical tweezers Raman spectroscopy system.
Results:1. The differences of RBC Raman spectra between umbilical cord blood and normal human are not obvious. 2. Compared with umbilical cord blood RBC, the overall intensity of Raman spectra in patients with congenital heart disease is significantly different. The overall spectrum of RBC in patients with congenital heart disease is weaker then umbilical cord blood RBC. Some characteristic peaks have a few displacements. Spectrum of 1273 cm-1 moved to 1264 cm-1; Intensity of spectrum of 1546 cm-1 reduced significantly. 3. RBC Raman spectra of normal human and patients with other heart disease :(1) Comparison between normal humans’RBC and RBC in patients with arrhythmias:①Some of the overall intensity from patients with arrhythmias is also weak.②The spectrum of 1453 cm-1 moved to 1449 cm-1.③The intensity of spectral of 863 cm-1 weakens and peak deformation widens the peaks of spectrum 1212 cm-1 and 1226 cm-1 combine into spectrum 1216 cm-1. In addition, the low intensity He-Ne laser irradiation on RBC in patients with arrhythmias has a certain impact. (2) Comparison the RBC between normal humans’and patients with myocardial infarction:①There was only little change in the overall intensity of two spectrums.②The intensity of spectrum 1273 cm-1 reduced significantly.③The spectrum of 1078 cm-1 moved to 1089 cm-1.
Conclusion:(1) Optical tweezers Raman spectroscopy can determine and analysis the difference of RBC between umbilical cord blood and patients with congenital heart disease and provide useful information for prenatal diagnosis, which may provide a new, quick and easy methods of spectral analysis for improving the detection rate of fetal congenital heart disease. (2) By comparing the Raman spectra of normal RBC and three kinds of RBC in patients with heart disease, we can understand the changes of RBC molecular structure, conformation and composition in different types of cardiovascular disease, and further study Raman spectra model of cardiovascular disease. We can also investigate the type and pathogenesis of cardiovascular disease from the molecular level and provide reliable experimental evidence for clinical diagnosis.
引文
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[3] Alexei V Feofanov,Alexei I Grichine,Larissa A Shitova et a1.Biophys.J,2000,7(8):499.
[4] Cheng Ji-Xin,Kevin Jia Y,Zheng Gengfeng et a1.Biophys.J.2002,83:502.
[5] ZHAO Hong-xia ,XU Yiming,ZHANG Zhi-yi.Chinese Science Bulletin(科学通报),1998,43(9):955.
[6] XU Yi-Ming,ZHANG Zhi-yi,ZHANG Wei.Science in China C(中国科学C),1998,28(5):417.
[7] SUN Chuan-Wen,xu Yi-zhuang,SUN Kai-hua et al (孙传文,徐怡庄,孙开华等).Spectroscopy and Spectral Analysis(光谱学与光谱分析),1996,16(5):22.
[8] YAN Xun-ling,WANG Qiu-guo,DONG Rui-xin(闫循领,王秋国,董瑞新).Spectro6copy and Spectral Analysis(光谱学与光谱分析) ,2003,23(6):1129.
[9] PuppelsG J,GarritsenH S,Segers-Nolten G M et a1.Biophys.J. 1991,60:1046.
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