羧甲基壳聚糖促牙周组织再生的毒理学研究
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摘要
目的:研究羧甲基壳聚糖应用于牙周组织的安全性。
方法:将32只昆明小鼠随机分为4组(每组8只),A为空白对照组,B为腹腔注射环磷酰胺阳性对照组,C、D为羧甲基壳聚糖梯度实验组,在手术骨缺损区置入梯度剂量的羧甲基壳聚糖。术后4周处死动物,应用彗星实验、嗜多染红细胞微核实验计算彗星尾数和微核率并进行统计学分析;收集牙周组织标本进行组织学观察。
结果:A组、C组、D组间红细胞微核率及彗星尾数无显著差异(P＞0.05),且C组与D组间亦无统计学差异(P＞0.05),而B组的红细胞微核率及彗星尾数远高于其余3组(P＜0.01)。术后4周C、D组骨缺损区可见少量新骨形成,A组未见新骨形成。
结论:羧甲基壳聚糖在该毒理学实验中未显示毒性结果,提示牙周组织局部应用羧甲基壳聚糖安全可靠;并具有促进牙周骨组织再生的作用。
Objective:To study the safety of N,0-Carboxymethy chitosan using in periodontal regeneration.
Method:32 mices were randomly divided into four groups with 8 rats in each group.Group A is negative group,B is positive group,C and D are different dose groups.N,0-Carboxymethy was implanted in mice's periodontal tissue of group C and D.Observed it's chronic and genetic toxicity by micronucleus,comet assay,tissue sections after 4 weeks.
Results:The number of micronucleus rate and comet tail in group B are much higher than A,C,D(p＜0.01);obvious difference wasn't exited between negative and test group(p＞0.05),moreover,low group C and D also exited little difference(p＞0.05).
Conclusion:N,0-Carboxymethy chitosan doesn't exit toxicity in appling to periodontal tis
方法:将32只昆明小鼠随机分为4组(每组8只),A为空白对照组,B为腹腔注射环磷酰胺阳性对照组,C、D为羧甲基壳聚糖梯度实验组,在手术骨缺损区置入梯度剂量的羧甲基壳聚糖。术后4周处死动物,应用彗星实验、嗜多染红细胞微核实验计算彗星尾数和微核率并进行统计学分析;收集牙周组织标本进行组织学观察。
结果:A组、C组、D组间红细胞微核率及彗星尾数无显著差异(P＞0.05),且C组与D组间亦无统计学差异(P＞0.05),而B组的红细胞微核率及彗星尾数远高于其余3组(P＜0.01)。术后4周C、D组骨缺损区可见少量新骨形成,A组未见新骨形成。
结论:羧甲基壳聚糖在该毒理学实验中未显示毒性结果,提示牙周组织局部应用羧甲基壳聚糖安全可靠;并具有促进牙周骨组织再生的作用。
Objective:To study the safety of N,0-Carboxymethy chitosan using in periodontal regeneration.
Method:32 mices were randomly divided into four groups with 8 rats in each group.Group A is negative group,B is positive group,C and D are different dose groups.N,0-Carboxymethy was implanted in mice's periodontal tissue of group C and D.Observed it's chronic and genetic toxicity by micronucleus,comet assay,tissue sections after 4 weeks.
Results:The number of micronucleus rate and comet tail in group B are much higher than A,C,D(p＜0.01);obvious difference wasn't exited between negative and test group(p＞0.05),moreover,low group C and D also exited little difference(p＞0.05).
Conclusion:N,0-Carboxymethy chitosan doesn't exit toxicity in appling to periodontal tis
引文
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2 Hurzelar MB,Quinones CR,Caffesse RG et al.Guided periodontal tissue regeneration in class Ⅲ furcation defects following treatment with a synthetic bioabsorbable barrier.J Periodontol 1997 May;68(5):498-505.
3 Somerman MJ,Ouyang HJ,Berry JE,et al.Evolution of periodontal regeneration:from the roots point of view[J].J periodont Res,1999,34:420-424.
4 O'Neal R,Wang HL,Macneil RL et al.Cells and materials involved in guided tissue regeneration.Curr Opin Priodontal,1994:141-156.
5 Corbet EF,Vaughan AJ,Kieser JB.The periodontally involved root surface.J Clin Periodontal,1993,20:402-410.
6 Crespi R,Ugo C,Joseph E,et al.Periodontal tissue regeneration in Beagie dogs after laser theraphy[J].Laser Surge Med,1997,21:395-402.
7 Kubota K,Yokota M,et al.Overview of effects of electrical stion on osteogenesis and alveolar bone[J].J periodontal,1995,66:2-6.
8 吴织芬.牙周病学第二版.北京:人民卫生出版社,2002
9 Ikada I.et.al.effect of chitosan hydrolysates on lipid absoption and on serum and liver lipid concentration in rats.J.Agr.Food chem.1993.41:431.
10 Brown KM.Arthur JR.Selenium.selenoproteins and human health:a review[J].Public Health Nutr.2001,(4):593-599
11 丘波,刘世清,杜予民等.壳聚糖膝关节腔注射疗法对兔骨关节炎关节软骨的影响.中华风湿学杂志,2004,8:591-595.
12 李志勇,孙建宁.水合氯醛和戊巴比妥钠对SD大鼠麻醉效果的比较[J].四川动物,2008,27(2):299-302.
13 刘静,于洪升.低剂量辐射对环磷酰胺所致小鼠DNA及遗传物质损伤的影响.青岛大学医学院学报,2008.44(1):44-47
14 邢卫平,赵恒奎,姜宗荣,等.微核及微核试验在遗传毒理学中的应用.安徽预防遗传杂志,2002,8:317-332.
15 Lekovic V,Camargo PM,Weinlaender M,et al.Acomparison between enamel matrix proteins,used alone or in combination with bovine porous bone mineral in the treatment of introbony barrier membranes periodontal defects in humans[J].J eriodontol,2000,71:1110-1116.
16 Vijislav L,Paulo M,Camargo,et al.Conbination use of bovineporous bone mineral,enamel matrix periodontal defects in humans[J],J Periodontal,2001,72(5):583-589.
17 Anton S,Giovanni C C,et al.Clinical evaluation of anenamel matrix protein derivative combined with a bioactive glass for the treatment of introbony periodontal defects in humans[J].J Periodontol,2002,73:40-408.
18 赵征,张广耘,吴织芬,等动态观察bFGF和rhBMP对牙周膜细胞增殖的影响.现代口腔医学杂志,2003,17(4):299.
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