天然抗菌成分盐酸小檗碱微胶囊的制备及抗菌性研究
摘要
黄柏作为一种功能性的天然中草药,其主要成分盐酸小檗碱具有优良的抗菌性能。本文以黄柏为原料,应用乙醇提取法提取盐酸小檗碱,在此基础上对提取进行优化,同时测试提取物的抗菌性能。通过研究微胶囊制备壁材的选用及乳化剂的用量、皮芯比、乳化时间、固化时间、乳化温度、搅拌速度几个因素对微胶囊制备的影响,得出最佳的制备工艺。采用SEM观测微胶囊的形貌,粒度分析仪测定微胶囊的粒度分布,红外光谱表征制备出的产品为盐酸小檗碱微胶囊,并测定了盐酸小檗碱微胶囊的缓释性能。论文的最后分别从微胶囊的整理用量、整理方式、耐洗性能几个方面测定了盐酸小檗碱微胶囊整理真丝织物的抑菌性能。
实验结果表明,常用的乙醇法提取盐酸小檗碱具有提取方便,纯度较高,成本低等特点,提取物经紫外光谱测定与标准品相符。
采用明胶-阿拉伯树胶为壁材制备的盐酸小檗碱微胶囊经SEM测定,其形貌图表面光滑平整,成规则的球形,效果较好。微胶囊的粒度的规律性较强,说明制备出的微胶囊有一定的稳定性。其红外光谱图表明明胶和阿拉伯树胶已经包结了盐酸小檗碱,胶囊产物得到验证。制备盐酸小檗碱微胶囊有较好的缓释性能,在时间为90min时,微胶囊的累积释放量达到80%以上,当时间再继续延长时盐酸小檗碱的累积释放量增长不快,将趋于稳定。
真丝织物的抑菌性能主要与整理到织物上的盐酸小檗碱微胶囊的量有关,微胶囊的最佳抑菌浓度为2g/L。整理后的真丝织物经过水洗5次后,金黄色葡萄球菌和大肠杆菌仍可以达到其MIC值。因而,将盐酸小檗碱微胶囊制成家用抗菌整理剂应用于家用纺织品的水洗中,具有一定的的市场前景。
Phellodendron is a function of natural herbs and its main component is berberine which has excellent antibacterial properties. In this paper, berberine was extracted from phellodendron by the means of Ethanol extraction. The extraction technics was optimized and the antibacterial properties of berberine were characterized. The effect of microencapsulation of wall material selection, as well as the amount of emulsifier、skin-core ratio、phacoemulsification time、curing time、emulsifying temperature、stirring speed on the microcapsules were considered to obtain the optimizing preparation technics. SEM and particle size analyzer were employed to observe the morphology and measure the particle size. Microencapsulation products were distinguished by IR and the extended release property was also measured. Finally, the effect of the microcapsule finishing dosage、finishing methods and washing performance on the antibacterial properties of silk fabrics finished by the microcapsule berberine were tested.
The results of experiment revealed that the ethanol extraction was a convenient and low cost way to extract berberine. The extraction is mainly composed of berberine whose UV-spectrum was same to standard sample.
The SEM micrography showed that the surface of berberine microcapsules was smooth and the capsules were standard spherical when Gelatin-Gum Arabic was used for the wall material. Microcapsule size had a better rule that certified the products stability. The results of IR indicated that berberine has been the inclusion of gelatin and gum-arabic. Berberine microcapsules have good extended release property. The release profile showed an initial burst that resulted in release of 80% of the original berberine amount after 90min, then a slow and steady rate release of berberine in the following time.
Antibacterial properties of silk fabric primarily were related to the amount of the berberine microcapsule, and the optimum antibacterial concentration was 2g/L. The pure silk fabrics, after 5 times wash, were still antibacterial to Staphylococcus aureus and Escherichia coli to MIC. The studies indicated the potential application of berberine microcapsule in household textile.
实验结果表明,常用的乙醇法提取盐酸小檗碱具有提取方便,纯度较高,成本低等特点,提取物经紫外光谱测定与标准品相符。
采用明胶-阿拉伯树胶为壁材制备的盐酸小檗碱微胶囊经SEM测定,其形貌图表面光滑平整,成规则的球形,效果较好。微胶囊的粒度的规律性较强,说明制备出的微胶囊有一定的稳定性。其红外光谱图表明明胶和阿拉伯树胶已经包结了盐酸小檗碱,胶囊产物得到验证。制备盐酸小檗碱微胶囊有较好的缓释性能,在时间为90min时,微胶囊的累积释放量达到80%以上,当时间再继续延长时盐酸小檗碱的累积释放量增长不快,将趋于稳定。
真丝织物的抑菌性能主要与整理到织物上的盐酸小檗碱微胶囊的量有关,微胶囊的最佳抑菌浓度为2g/L。整理后的真丝织物经过水洗5次后,金黄色葡萄球菌和大肠杆菌仍可以达到其MIC值。因而,将盐酸小檗碱微胶囊制成家用抗菌整理剂应用于家用纺织品的水洗中,具有一定的的市场前景。
Phellodendron is a function of natural herbs and its main component is berberine which has excellent antibacterial properties. In this paper, berberine was extracted from phellodendron by the means of Ethanol extraction. The extraction technics was optimized and the antibacterial properties of berberine were characterized. The effect of microencapsulation of wall material selection, as well as the amount of emulsifier、skin-core ratio、phacoemulsification time、curing time、emulsifying temperature、stirring speed on the microcapsules were considered to obtain the optimizing preparation technics. SEM and particle size analyzer were employed to observe the morphology and measure the particle size. Microencapsulation products were distinguished by IR and the extended release property was also measured. Finally, the effect of the microcapsule finishing dosage、finishing methods and washing performance on the antibacterial properties of silk fabrics finished by the microcapsule berberine were tested.
The results of experiment revealed that the ethanol extraction was a convenient and low cost way to extract berberine. The extraction is mainly composed of berberine whose UV-spectrum was same to standard sample.
The SEM micrography showed that the surface of berberine microcapsules was smooth and the capsules were standard spherical when Gelatin-Gum Arabic was used for the wall material. Microcapsule size had a better rule that certified the products stability. The results of IR indicated that berberine has been the inclusion of gelatin and gum-arabic. Berberine microcapsules have good extended release property. The release profile showed an initial burst that resulted in release of 80% of the original berberine amount after 90min, then a slow and steady rate release of berberine in the following time.
Antibacterial properties of silk fabric primarily were related to the amount of the berberine microcapsule, and the optimum antibacterial concentration was 2g/L. The pure silk fabrics, after 5 times wash, were still antibacterial to Staphylococcus aureus and Escherichia coli to MIC. The studies indicated the potential application of berberine microcapsule in household textile.
引文
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