肠易激综合征模型的建立及痛泻要方对其血液和结肠组织中VIP、SS影响的实验研究
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摘要
目的:建立肠易激综合征(irritable bowel syndrome, IBS)动物模型,研究痛泻要方对其血液和结肠组织中血管活性肠肽(vasoactive intestinal peptide,VIP)和生长抑素(Somatostatin,SS)的影响。
方法:清洁级SD大鼠42只,应用慢性结肠刺激与夹尾刺激相结合的方法,建立肠道高敏的IBS模型并对其进行评估。验证造模成功后,重新选用清洁级健康新生SD大鼠120只,将实验大鼠随机分为空白对照组(A组)、模型对照组(B组)、中药低剂量组(C组)、中药中剂量组(D组)、中药高剂量组(E组)、西药斯巴敏组(F组),依上述方法进行造模并应用中药痛泻要方对其治疗,与西药斯巴敏对照,观察其对模型大鼠血液和结肠组织中VIP及SS的影响。
结果:(l)IBS模型大鼠在0.3、0.6、0.9 ml的直肠扩张容量下,与新生期生理盐水刺激组及新生期醋酸刺激组相比,新生期醋酸刺激加夹尾刺激组大鼠腹部抬起和背部拱起的容量阈值显著降低(P值均<0.01);(2)在测定IBS模型大鼠腹壁肌电活动时,与新生期醋酸刺激组相比,新生期醋酸刺激加夹尾刺激组大鼠腹壁肌电活动明显增强(P值均<0.05);与新生期生理盐水刺激组相比,新生期醋酸刺激加夹尾刺激组(P分别<0.01、<0.05和0.05)。(3)模型大鼠血浆及结肠中VIP及SS含量明显增加,高剂量痛泻要方和西药斯巴敏均能显著降低血浆及结肠组织SS含量(P<0.05),痛泻要方低剂量组、中剂量组效果不显著。
结论:(l)通过对IBS模型大鼠行为学、电生理指标及结肠病理切片的观察表明:慢性结肠刺激与夹尾刺激相结合的方法建立的肠道高敏IBS模型可以更好地模拟其病因和发病机制。(2)高剂量痛泻要方使IBS模型大鼠血浆和结肠组织中VIP、SS含量降低,使其趋于正常可能是该方治疗肠易激综合征的作用机制之一。
Objective:
Sets up a irritable bowel syndrome model, and Tongxiey- aofang is used to research effect of vasoactive intestinal peptide and Somatostatin in the artery and Colon of irri- table bowel syndrome model.
Methods:
Totally 42 neonatal SD rats is selected. With help of adopting by chronic stimulation rats in colon and on tail to sets up the model of intestinal hypersensitivity and to evaluate model .After verification establishing model successful, totally 120 neonatal SD rats is anew selected and that were randomly divided into six groups: the addition of the normal control group (Group A),the model group(Group B),the low dosage of Tongxieyaofang group (Group C),the middle dosage of Tongxieyaofang group (Group D),the high dosage of Tongxieyaofang group (Group E), with the Spasmomen control group(Group F).Treatment of IBS model rats by tongxieyaofang,and compare with Spasmomen,to observe effect that is vasoactive intestinal peptide and Somatostatin in the artery and Colon of model rats.
Results:
(1) In contrast to neonatal rats subjected to saline intrarectally (A group) and neonatal rats subjected to acetic acid intrarectally (B group), neonatal rats subjected to acetic acid intrarectally and stimulated with forceps clip on tails (C group) showed a significant decrease in the volume threshold of abdomen lifting and body arching during rectal distension(P<0.01). (2)There was a significant increase of abdominal electrical activity responding to 0.3, 0.6, and 0.9mL distention volume. (Compared with B group P<0.05, P<0.05 and P<0.05, respectively. Compared with A group P<0.01, P<0.05 and P<0.05, respectively). (3) model of plasma in the colon and VIP and SS were significantly increased, high-dose pain, diarrhoea and western medicine to Siba Min can significantly reduce plasma and colon SS concentration (P <0.05), pain, diarrhoea to low-dose Group, the dose effect not significant.
Conclusions:
(l) by IBS rat model behavior, physiology, pathology section of colon and indicators of the observation that: chronic colon and stimulate the end folder to stimulate the establishment of the method of combining the high sensitivity of intestinal IBS model can better simulate the etiology and pathogenesis. (2) High-dose pain to diarrhea side IBS model of plasma and colon in VIP, SS was reduced, it tends to be normal, the treatment of irritable bowel syndrome in one of the mechanisms.
方法:清洁级SD大鼠42只,应用慢性结肠刺激与夹尾刺激相结合的方法,建立肠道高敏的IBS模型并对其进行评估。验证造模成功后,重新选用清洁级健康新生SD大鼠120只,将实验大鼠随机分为空白对照组(A组)、模型对照组(B组)、中药低剂量组(C组)、中药中剂量组(D组)、中药高剂量组(E组)、西药斯巴敏组(F组),依上述方法进行造模并应用中药痛泻要方对其治疗,与西药斯巴敏对照,观察其对模型大鼠血液和结肠组织中VIP及SS的影响。
结果:(l)IBS模型大鼠在0.3、0.6、0.9 ml的直肠扩张容量下,与新生期生理盐水刺激组及新生期醋酸刺激组相比,新生期醋酸刺激加夹尾刺激组大鼠腹部抬起和背部拱起的容量阈值显著降低(P值均<0.01);(2)在测定IBS模型大鼠腹壁肌电活动时,与新生期醋酸刺激组相比,新生期醋酸刺激加夹尾刺激组大鼠腹壁肌电活动明显增强(P值均<0.05);与新生期生理盐水刺激组相比,新生期醋酸刺激加夹尾刺激组(P分别<0.01、<0.05和0.05)。(3)模型大鼠血浆及结肠中VIP及SS含量明显增加,高剂量痛泻要方和西药斯巴敏均能显著降低血浆及结肠组织SS含量(P<0.05),痛泻要方低剂量组、中剂量组效果不显著。
结论:(l)通过对IBS模型大鼠行为学、电生理指标及结肠病理切片的观察表明:慢性结肠刺激与夹尾刺激相结合的方法建立的肠道高敏IBS模型可以更好地模拟其病因和发病机制。(2)高剂量痛泻要方使IBS模型大鼠血浆和结肠组织中VIP、SS含量降低,使其趋于正常可能是该方治疗肠易激综合征的作用机制之一。
Objective:
Sets up a irritable bowel syndrome model, and Tongxiey- aofang is used to research effect of vasoactive intestinal peptide and Somatostatin in the artery and Colon of irri- table bowel syndrome model.
Methods:
Totally 42 neonatal SD rats is selected. With help of adopting by chronic stimulation rats in colon and on tail to sets up the model of intestinal hypersensitivity and to evaluate model .After verification establishing model successful, totally 120 neonatal SD rats is anew selected and that were randomly divided into six groups: the addition of the normal control group (Group A),the model group(Group B),the low dosage of Tongxieyaofang group (Group C),the middle dosage of Tongxieyaofang group (Group D),the high dosage of Tongxieyaofang group (Group E), with the Spasmomen control group(Group F).Treatment of IBS model rats by tongxieyaofang,and compare with Spasmomen,to observe effect that is vasoactive intestinal peptide and Somatostatin in the artery and Colon of model rats.
Results:
(1) In contrast to neonatal rats subjected to saline intrarectally (A group) and neonatal rats subjected to acetic acid intrarectally (B group), neonatal rats subjected to acetic acid intrarectally and stimulated with forceps clip on tails (C group) showed a significant decrease in the volume threshold of abdomen lifting and body arching during rectal distension(P<0.01). (2)There was a significant increase of abdominal electrical activity responding to 0.3, 0.6, and 0.9mL distention volume. (Compared with B group P<0.05, P<0.05 and P<0.05, respectively. Compared with A group P<0.01, P<0.05 and P<0.05, respectively). (3) model of plasma in the colon and VIP and SS were significantly increased, high-dose pain, diarrhoea and western medicine to Siba Min can significantly reduce plasma and colon SS concentration (P <0.05), pain, diarrhoea to low-dose Group, the dose effect not significant.
Conclusions:
(l) by IBS rat model behavior, physiology, pathology section of colon and indicators of the observation that: chronic colon and stimulate the end folder to stimulate the establishment of the method of combining the high sensitivity of intestinal IBS model can better simulate the etiology and pathogenesis. (2) High-dose pain to diarrhea side IBS model of plasma and colon in VIP, SS was reduced, it tends to be normal, the treatment of irritable bowel syndrome in one of the mechanisms.
引文
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