福建烟蚜抗药性研究
摘要
本文应用吡虫啉、好年冬和氰戊菊酯3种杀虫剂对烟蚜种群进行敏感性测定,测定结果表明,在福州4个不同地方的烟蚜种群中,这3种农药均对福州宦溪的烟蚜种群毒力最高,由此选定福州宦溪烟蚜种群作为相对敏感品系。测定了福建省3个烟区(三明、南平和龙岩)烟蚜种群羧酸酯酶、乙酰胆碱酯酶和谷胱甘肽-S-转移酶的活性,结果表明,这3个烟区烟蚜种群的3种酶活性差异明显,以三明种群最高,南平种群次之,龙岩种群最低。测定了氰戊菊酯、好年冬和吡虫啉在不同温度条件下对烟蚜的毒力,结果表明,好年冬和毗虫啉的LC_(50)与温度呈正相关,其中吡虫啉受温度影响较大,35℃的LC_(50)是15℃的LC_(50)的7.124倍;好年冬次之,35℃的LC_(50)是15℃的LC_(50)的2.882倍;氰戊菊酯的LC_(50)与温度呈负相关,15℃的LC_(50)是35℃的LC_(50)的5.048倍。系统研究了取食不同寄主植物的烟蚜对药剂敏感性的差异,结果表明,取食不同寄主的烟蚜种群抗性从强到弱依次为:甘蓝种群、龙葵种群、烟草种群和桃树种群;羧酸酯酶、乙酰胆碱酯酶和谷光甘肽-S-转移酶活性依次为:甘蓝种群〉龙葵种群〉烟草种群〉桃树种群,表明这3种酶活性差异是造成取食不同寄主的烟蚜对药剂敏感性差异的重要原因。
The sensitive tests of Myzus persicae(Sulzer)to 3 insecticides,imidacloprid,carbosulfan and fenvalerate,were observated.The result showed that the aphid sensitivity of Fuzhou Huanxi population was the highest in 4 different populations,so Fuzhou Huanxi population was selected as the comparatively sensitive population.The activities of AChE,CarE and GSTs of the aphid population from 3 main planting tobacco areas of Fujian were determined, the results showed that the activities of 3 enzymes were obviously different,and the order of activities of 3 enzymes were the Sanming population＞the Nanping population＞the Lonyyan population.Then the toxicities of 3 insecticides to the aphids were studied at different tempetatures.For imidacloprid and carbosulfan,from 15℃to 35℃,the relation between the toxicity and the temperature was positive correlation.The toxicity of imidacloprid of 15℃was 7.124 times high as that at 35℃;the toxicity of carbosulfan of 15℃was 2.882 times high as that at 35℃.for fenvalerate,the relation between the toxicity and the temperature was negative correlation,the toxicity of imidacloprid of 35℃was 5.048 times high as that at 15℃. The resistant diversities of M.persicae on different hosts and their resistant mechanisms were studied.The results showed the toxicity order of the aphid populations on different hosts was cabbage population＞morel population＞tobacco population＞peach population.The activity order of ACHE,GSTs and CarE were cabbage population＞morel population＞tobacco population＞peach population.The activity differences of 3 enzymes were important reasons for the activities of insecticides on M.persicae on different hosts.
The sensitive tests of Myzus persicae(Sulzer)to 3 insecticides,imidacloprid,carbosulfan and fenvalerate,were observated.The result showed that the aphid sensitivity of Fuzhou Huanxi population was the highest in 4 different populations,so Fuzhou Huanxi population was selected as the comparatively sensitive population.The activities of AChE,CarE and GSTs of the aphid population from 3 main planting tobacco areas of Fujian were determined, the results showed that the activities of 3 enzymes were obviously different,and the order of activities of 3 enzymes were the Sanming population＞the Nanping population＞the Lonyyan population.Then the toxicities of 3 insecticides to the aphids were studied at different tempetatures.For imidacloprid and carbosulfan,from 15℃to 35℃,the relation between the toxicity and the temperature was positive correlation.The toxicity of imidacloprid of 15℃was 7.124 times high as that at 35℃;the toxicity of carbosulfan of 15℃was 2.882 times high as that at 35℃.for fenvalerate,the relation between the toxicity and the temperature was negative correlation,the toxicity of imidacloprid of 35℃was 5.048 times high as that at 15℃. The resistant diversities of M.persicae on different hosts and their resistant mechanisms were studied.The results showed the toxicity order of the aphid populations on different hosts was cabbage population＞morel population＞tobacco population＞peach population.The activity order of ACHE,GSTs and CarE were cabbage population＞morel population＞tobacco population＞peach population.The activity differences of 3 enzymes were important reasons for the activities of insecticides on M.persicae on different hosts.
引文
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