摘要
采用盆栽模拟试验方法,通过对叶水势、叶片相对含水量、可溶性蛋白含量、脯氨酸含量、丙二醛含量、细胞质膜相对透性和气体交换参数等抗旱生理生化指标的测定,对不同浓度的Vc、水杨酸、多效唑叶面喷施处理的紫丁香、小叶锦鸡儿、乌苏里绣线菊和沙棘苗木的抗旱能力进行了分析和综合评价,研究结果表明:(1)Vc、水杨酸、多效唑均能通过不同途径提高四树种的抗旱能力。在干旱条件下,三种植物生长调节剂均能通过不同程度地延缓四树种的叶水势、叶片相对含水量、可溶性蛋白质含量、叶绿素含量和净光合作用的下降,提高叶片的脯氨酸含量,延缓植物在干旱条件下细胞膜脂过氧化作用和细胞质膜相对透性的发生,提高试验苗木的抗旱性。(2)提高四树种抗旱能力的适宜生长调节剂种类及浓度不同:紫丁香为5.5×10~(-3)-8.5×10~(-3)mol/L的VC和0.7×10~(-4)-1.0×10~(-4)mol/L的水杨酸;小叶锦鸡儿为8.5×10~(-3)mol/L的Vc和0.4×10~(-4)-0.7×10~(-4)mol/L的水杨酸;乌苏里绣线菊为1.53×10~(-3)-2.21×10~(-3)mol/L的多效唑;沙棘为0.4×10~(-4)-1.0×10~(-4)mol/L的水杨酸。其结果为抗旱苗木的定向培育和提高干旱、半干旱地区造林效果奠定了基础。
The changes of leaf water potential, relative water content, the content of soluble protein, proline and malondialdehyde, relative permeability of plasma membrance and gas exchange parameters were measured by means of the simulated pot experiments. The abilities of drought resistance of Syringa oblate, Caranana microphylla, Spiraea ussuriensis, Hippophae rhamnoides in the course of seedling were studied by spraying four contents of different plant growth regulators including ascorbic acid, salicylic acid and paclobutrazol. The results showed as foliowed.(1) The three kinds of plant growth regulators could increase the abilities of drought resistance of all the shrub seedlings via different ways. Under the condition of drought, all the plant growth regulators could effectively postpone the fall of leaf water potential, relative water content, the content soluble proteins and chlorophyll and net photosynthesis and the occurs of lipid peroxidation and membrance permeability to some extent. In addition, the content of proline in leaves was increased. (2) The most suited kind and content of the plant growth regulators in enhancing the abilities of drought resistance were different for the four kinds of shrub seedlings. 5.5x10-3 - 8.5x10-3mol/L ascorbic acid and 0.7x104 - 1.0x10-4 mol/L salicylic acid were the most appropriate for Syringa oblate, 8.5x10-3mol/L ascorbic acid and 0.4x10-4 - 0.7x10-4mol/L salicylic acid for Caranana microphylla, 1.53x10-3 - 2.21x10-3mol/L paclobutrazol for Spiraea ussuriensi and 0.4x10-4- 1.0x10-4mol/L salicylic acid for Hippophae rhamnoide. The results could establish a definite base for directional cultivating and afforestation in arid and semiarid areas.
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