含对虾白斑综合症病毒ie1启动子的重组杆状病毒介导的哺乳动物细胞基因转移
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摘要
随着携带哺乳动物细胞启动子的重组杆状病毒逐渐成为基因功能研究、非复制型载体疫苗研制以及开发基因治疗策略的一个非常有效的工具,寻找一种在昆虫细胞和哺乳动物细胞之间的穿梭启动子对于杆状病毒介导的基因转移方面的研究非常必要。
在本研究中,我们利用对虾白斑综合症病毒(white spot syndrome virus,WSSV)的ie1启动子和杆状病毒ETL启动子以及杆状病毒多角体启动子,分别构建了含有不同启动子调控下的EGFP报告基因表达盒的重组杆状病毒,然后转导不同哺乳动物细胞,以检测不同启动子在细胞中的转导和表达效率。结果显示,WSSV的ie1启动子在昆虫细胞sf9中具有很强的启动子活性,能够控制报告基因的高效表达,同时在哺乳动物细胞中,ie1启动子与ETL启动子一样在不同哺乳动物细胞中均有活性,能够介导EGFP不同水平的表达,并且丁酸钠能够提高ie1启动子在哺乳动物细胞中的转导和表达效率。转移载体瞬时转染结果证实,ie1启动子能够直接启动外源基因在哺乳动物细胞中的表达,而不依赖于杆状病毒基因表达。
本研究首次发现ie1是一种杆状病毒非依赖性、在昆虫细胞和哺乳动物细胞之间的新型穿梭启动子,该穿梭启动子对于杆状病毒表达载体在基因治疗和非复制型载体疫苗方面的研究将具有重要应用价值。
As recombinant baculoviruses containing mammalian cell-active promoters are becoming a promising tool for studying gene functions and developing non-replicative vector vaccines and gene therapy strategies, a shuttle promoter that mediates gene expression in both insect cells and mammalian cells will facilitate the development of a baculovirus vector-based mammalian cell gene delivery vehicle.
In this report, three recombinant baculoviruses were generated carrying the reporter EGFP gene under the control of white spot syndrome virus (WSSV) ie1 promoter, or either of two control promoters, baculovirus early to late (ETL) promoter and polyhedrin promoter. Several mammalian cell lines were transduced with the resulting recombinant baculoviruses to test the expression of EGFP. The results showed that WSSV ie1 promoter showed a strong promoter activity in insect cells and mammalian cells. The promoter activity of the ie1 promoter but not ETL promoter can be enhanced by sodium butyrate, a histone deacetylase inhibitor. Transient plasmid transfection assay indicated that ie1 promoter activity in mammalian cells is independent on baculovirus gene expression, which is different from ETL promoter, a baculovirus-dependent shuttle promoter.
This study demonstrated, for the first time, that ie1 is a baculovirus-independent shuttle promoter between insect cells and mammalian cells. This novel shuttle promoter will facilitate the application of baculovirus-based vectors in gene expression, gene therapy and non-replicative vector vaccines.
在本研究中,我们利用对虾白斑综合症病毒(white spot syndrome virus,WSSV)的ie1启动子和杆状病毒ETL启动子以及杆状病毒多角体启动子,分别构建了含有不同启动子调控下的EGFP报告基因表达盒的重组杆状病毒,然后转导不同哺乳动物细胞,以检测不同启动子在细胞中的转导和表达效率。结果显示,WSSV的ie1启动子在昆虫细胞sf9中具有很强的启动子活性,能够控制报告基因的高效表达,同时在哺乳动物细胞中,ie1启动子与ETL启动子一样在不同哺乳动物细胞中均有活性,能够介导EGFP不同水平的表达,并且丁酸钠能够提高ie1启动子在哺乳动物细胞中的转导和表达效率。转移载体瞬时转染结果证实,ie1启动子能够直接启动外源基因在哺乳动物细胞中的表达,而不依赖于杆状病毒基因表达。
本研究首次发现ie1是一种杆状病毒非依赖性、在昆虫细胞和哺乳动物细胞之间的新型穿梭启动子,该穿梭启动子对于杆状病毒表达载体在基因治疗和非复制型载体疫苗方面的研究将具有重要应用价值。
As recombinant baculoviruses containing mammalian cell-active promoters are becoming a promising tool for studying gene functions and developing non-replicative vector vaccines and gene therapy strategies, a shuttle promoter that mediates gene expression in both insect cells and mammalian cells will facilitate the development of a baculovirus vector-based mammalian cell gene delivery vehicle.
In this report, three recombinant baculoviruses were generated carrying the reporter EGFP gene under the control of white spot syndrome virus (WSSV) ie1 promoter, or either of two control promoters, baculovirus early to late (ETL) promoter and polyhedrin promoter. Several mammalian cell lines were transduced with the resulting recombinant baculoviruses to test the expression of EGFP. The results showed that WSSV ie1 promoter showed a strong promoter activity in insect cells and mammalian cells. The promoter activity of the ie1 promoter but not ETL promoter can be enhanced by sodium butyrate, a histone deacetylase inhibitor. Transient plasmid transfection assay indicated that ie1 promoter activity in mammalian cells is independent on baculovirus gene expression, which is different from ETL promoter, a baculovirus-dependent shuttle promoter.
This study demonstrated, for the first time, that ie1 is a baculovirus-independent shuttle promoter between insect cells and mammalian cells. This novel shuttle promoter will facilitate the application of baculovirus-based vectors in gene expression, gene therapy and non-replicative vector vaccines.
引文
1. Smith GE, Summers MD, Fraser MJ. Production of human beta-interferon in insect cells infected with a baculovirus expression vector. Mol Cell Biol, 1983, 3(12): 2156-65.
2. Maeda S, Kawai T, Obinata M, et al. Production of human alpha-interferon in silkworm using a baculovirus vector. Nature, 1985, 315(6020): 592-594.
3. Sriram S, Palhan VB, Gopinathan KP. Heterologous promoter recognition leading to high-level expression of cloned foreign genes in Bombyx mori cell lines and larvae. Gene, 1997, 190(1): 181-189.
4. Cha HJ, Dalal NG, Vakharia VN, et a1. Expression and purification of human interleukin-2 simplified as a fusion with green fluorescent protein in suspended sf-9 insect cells. Biotechnology, 1999, 69(1): 9-17.
5. 卢兹凡, 陈苏民, 陈南春等. 人 BMP-2 在昆虫杆状病毒表达系统中的表达及初步鉴定. 细胞与分子免疫学杂志, 1999, l5(3):170-172.
6. Vlak JM, Keus RJ. Baculovirus expression vector system for production of viral vaccines. Adv Biotechnol Processes, 1990, 14: 91-128.
7. Ailor E, Betenbaugh MJ. Modifying secretion and post-translational processing in insect cells. Curr Opin Biotechnol, 1999, 10(2): 42-145.
8. Joshi L, Davis TR, Mattu TS, et a1. Influence of baculovirus-host cell interactions on complex N-linked glycosylation of a recombinant human protein. Biotechnol Prog, 2000, 16(4): 650-656.
9. Dai X, Hajos JP, Joosten NN, et a1. Isolation of a Spodoptera exigua baculovirus recombinant with a 10.6 kbp genome deletion that retains biological activity. J Gen Virol, 2000, 81(10): 2545-54.
10. Sandoval C, Curtis H, Congote LF. Enhanced proliferative effects of a baculovirus-produced fusion protein of insulin-like growth factor and alpha(1)-proteinase inhibitor and improved anti-elastase activity of the inhibitor with glutamate at position 351. Protein Eng, 2002, 15(5): 413-418.
11. 郭君惠, 杜昌生, 彭建新等. 重组杆状病毒安全性及其评价. 中国生物防治, 2003, 19 (1): 40-43.
12. Kost TA, Condreay JP, Jarvis DL. Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nature Biotechnology, 2005, 23(5): 567-575.
13. Kitts PA, Ayres MD, Possee RD. Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acids Res, 1990, 18(19): 5667-5672.
14. Luckow VA, Lee SC, Barry GF, et al. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J Virol, 1993, 67(8): 4566-79.
15. Invitrogen life technologies instruction Manual. Guide to Baculovirus Expression Vector system (BEVS) and insect cell culture techniques.
16. DiFalco MR, Bakopanos E, Patricelli M, et al. The influence of various insect cell lines, p10 and polyhedrin promoters in the production of secreted insulin-like growth factor-interleukin-3 chimeras in the baculovirus expression system. J Biotechnol, 1997, 56(1): 49-56.
17. Zuidema D, Klinge-Roode EC, van Lent JW, et al. Construction and analysis of an Autographa californica nuclear polyhedrosis virus mutant lacking the polyhedral envelope. Virology, 1989, 173(1): 98-108.
18. Vialard J, Lalumiere M, Vernet T, et al. Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector containing the beta-galactosidase gene. J Virol, 1990, 64(1): 37-50.
19. Thiem S M, Miller LK. Differential gene expression mediated by late, very late and hybrid baculovirus promoters. Gene, 1990, 91(1): 87-94.
20. Vlak JM, Klinkenberg FA, Zaal KJ, et al. Functional studies on the p10 gene of Autographa californica nuclear polyhedrosis virus using a recombinant expressing a p10-beta-galactosidase fusion gene. J Gen Virol, 1988, 69(4): 765-776.
21. Hill-Perkins MS, Possee RD. A baculovirus expression vector derived from the basic protein promoter of Autographa californica nuclear polyhedrosis virus. J Gen Virol, 1990,71 (4): 971-6.
22. Liu YK, Chu CC, Wu TY. Baculovirus ETL promoter acts as a shuttle promoter between insect cells and mammalian cells. Acta Pharmacol Sin, 2006, 27(3): 321-7.
23. Daniela M, Andreas K, Dagmar KM. Baculovirus transactivator IE1 is functional in mammalian cells. J Gen Virol, 1997, 78(6): 1507-10.
24. Liu WJ, Chang YS, Wang CH, et al. Microarray and RT-PCR screening for white spot syndrome virus immediate-early genes in cycloheximide-treated shrimp. Virology, 2005, 334(2): 327-341.
25. Liu WJ, Chang YS, Wang AH, et al. White spot syndrome virus annexes a shrimp STAT to enhance expression of the immediate-early gene ie1. J Virol, 2007, 81(3): 1461-71.
26. 朱江, 吴祥甫. 昆虫杆状病毒表达系统研究进展及其应用展望. 蚕业科学, 2003, 29(2): 114-119.
27. 贾帅争. 国外医学分子生物学分册, 2000, 22(1): 49-52.
28. 陈茹. 中国进出境动植物检疫, 1997, 4: 36-38.
29. 王立良. 国外医学分子生物学分册, 1998, 20(5): 222-226.
30. Drings A, Jallet C, Chambert B, et al. Is there an advantage to including the nucleoprotein in a rabies glycoprotein subunit vaccine? Vaccine, 1999, 17(11-12): 1549-57.
31. Touze A, Dupuy C, Chabaud M, et al. Production of human papillomavirus type 45 virus-like particles in insect cells using a recombinant baculovirus. FEMS Microbiol Lett, 1996, 141(1): 111-6.
32. Wang YH, Davies AH, Jones IM. Expression and purification of glutathione S-transferase-tagged HIV-1 gp120: no evidence of an interaction with CD26. Virology, 1995, 208(1): 142-6.
33. Tobin GJ, Nagashima K, Gonda MA. Immunologic and ultrastructural characterization of HIV pseudovirions containing Gag and Env precursor proteins engineered in insect cells. Methods, 1996, 10(2): 208-218.
34. Crawford J, Wilkinson B, Vosnesensky A, et al. Baculovirus-derived hemagglutinin vaccines protect against lethal influenza infections by avian H5 and H7 subtypes. Vaccine, 1999, 17(18): 2265-74.
35. Meyer RF, Babcock GD, Newman JF, et al. Baculovirus expressed 2C of foot-and-mouth disease virus has the potential for differentiating convalescent from vaccinated animals. J Virol Methods, 1997, 65(1): 33-43.
36. Shi YP, Das P. Development, expression and murine testing of a multistage Plasmodium falciparum malaria vaccine candidate. Vaccine, 2000, 18(25): 2902-14.
37. Hota-Mitchell S, Siddiqui AA, Dekaban GA, et al. Protection against Schistosoma mansoni infection with a recombinant baculovirus-expressed subunit of calpain. Vaccine, 1997, 15(15): 1631-40.
38. Bae JE, Schurig GG, Toth TE. Mice immune responses to Brucella abortus heat shock proteins. Use of baculovirus recombinant-expressing whole insect cells, purified Brucella abortus recombinant proteins, and a vaccinia virus recombinant as immunogens. Vet Microbiol, 2002, 88(2): 189-202.
39. 许辰煜, 程通, 卢五迅等. 杆状病毒对不同哺乳动物细胞基因转移及表达效率的研究. 生物工程学报, 2004, 20(1): 73-77.
40. Boyce FM, Bucher NL. Baculovirus mediated gene transfer into mammalian cells. Proc Natl Acad Sci USA, 1996, 93(6): 2348-52.
41. Condreay JP, Witherspoon SM, Clay WC, et al. Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc Natl Acad Sci USA, 1999, 96: 127-32.
42. Airenne KJ, Hiltunen MO, Turunen MP, et al. Baculovirus-mediated periadventitial gene transfer to rabbit carotid artery. Gene Ther, 2000, 7(17): 1499-504.
43. Liu X, Li K, Song J, et al. Efficient and stable gene expression in rabbit intervertebral disc cells transduced with a recombinant baculovirus vector. Spine, 2006, 31(7): 732-735.
44. Forstova J, Krauzewicz N, Sandig V, et al. Polyoma virus pseudocapsids as efficient carriers of heterologous DNA into mammalian cells. Hum Gene Ther, 1995, 6(3): 297-306.
45. Lehtolainen P, Tyynela K, Kannasto J, et al. Baculoviruses exhibit restricted cell type specificity in rat brain: a comparison of baculovirus- and adenovirus-mediated intracerebral gene transfer in vivo. Gene Ther, 2002, 9(24): 1693-9.
46. Sandig V, Hofmann C, Steinert S, et al. Gene transfer into hepatocytes and human liver tissue by baculovirus vectors. Hum Gene Ther, 1996, 7(16): 1937-1945.
47. Hofmann C, Strauss M. Baculovirus-mediated gene transfer in the presence of human serum or blood facilitated by inhibition of the complement system. Gene Ther, 1998, 5(4): 531-536.
48. Pieroni L, Maione D, La Monica N. In vivo gene transfer in mouse skeletal muscle mediated by baculovirus vectors. Hum Gene Ther, 2001, 12(8): 871-881.
49. Sarkis C, Serguera C, Petres S, et al. Efficient transduction of neural cells in vitro and in vivo by a baculovirus-derived vector. Proc Natl Acad Sci USA, 2000, 97: 14638-14643
50. Volkman LE, Goldsmit PA. In Vitro Survey of Autographa californica Nuclear Polyhedrosis Virus Interaction with Nontarget Vertebrate Host Cells. Appl Environ Microbiol, 1983, 45(3): 1085-1093.
51. Hofmann C, Sandig V, Jennings G, et al. Efficient gene transfer into human hepatocytes by baculovirus vectors. Proc Natl Acad Sci USA, 1995, 92(22): 10099-103.
52. Boyce FM, Butcher NL. Baculovirus-mediated gene transfer into mammalian cells. Proc Natl Acad Sci USA, 1996, 93(6): 2348-52.
53. Shoji I, Aizaki H, Tani H, et al. Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors. J Gen Virol, 1997, 78 (10): 2657-64
54. Ma L, Tamarina N, Wang Y, et al. Baculovirus-mediated gene transfer into pancreatic islet cells. Diabetes, 2000, 49(12): 1986-91.
55. Wagle M, Jesuthasan S. Baculovirus-mediated gene expression in zebrafish. Mar Biotechnol (NY), 2003, 5(1): 58-63.
56. Cheng T, Xu CY, Wang YB, et al. A rapid and efficient method to express target genes in mammalian cells by baculovirus. World J Gastroenterol, 2004, 10(11): 1612-8.
57. Dee KU, Shuler ML. Optimization of an assay for baculovirus titer and design of regimens for the synchronous infection of insect cells. Biotechnol Prog, 1997, 13(1): 14-24.
58. 梁昌墉, 胡志红, 陈新文. 杆状病毒作为基因治疗载体的研究进展. 生物化学与生物物理进展, 2002, 29 (6): 860-862.
59. Duisit G, Saleun S, Douthe S, et al. Baculovirus vector requires electrostatic interactions including heparan sulfate for efficient gene transfer in mammalian cells. J Gene Med, 1999, 1(2): 93-102.
60. Van Loo ND, Fortunati E, Ehlert E, et al. Baculovirus infection of nondividing mammalian cells: mechanisms of entry and nuclear transport of capsids. J Virol, 2001, 75(2): 961-70.
61. Hofmann C, Hermanns W, et al. Demonstration of cells of the mononuclear phagocyte system of the cat using enzyme and immunohistochemistry methods. Berl Munch Tierarztl Wochenschr, 1995, 108(3): 81-7.
62. Tani H, Nishijima M, Ushijima H, et al. Characterization of cellsurface determinants important for baculovirus infection. Virology, 2001, 279(1): 343-353.
63. Bilelo JP, Cable EE, Myers RL, et al. Role of paracellular junction complexes in baculovirus-mediated gene transfer to nondividing rat hepatocytes. Gene Ther, 2003, 10(9): 733-749.
64. Barsoum J, Brown R, McKee M, et al. Efficient transduction of mammalian cells by a recombinant baculovirus having the vesicular stomatitis virus G glycoprotein. Hum Gene Ther, 1997, 8(17): 2011-8.
65. Kukkonen SP, Airenne KJ, Marjomaki V, et al. Baculovirus capsid display: a novel tool for transduction imaging. Mol Ther, 2003, 8(5): 853-62.
66. Ho YC, Chung YC, Hwang SM, et al. Transgene expression and differentiation of baculovirus-transduced human mesenchymal stem cells. J Gene Med, 2005, 7(7): 860-8.
67. Lo HR, Chou CC, Wu TY, et al. Novel baculovirus DNA elements strongly stimulate activities of exogenous and endogenous promoters. J Biol Chem, 2002, 277(7): 5256-64.
68. Venkaiah B, Viswanathan P, Habib S, et al. An additional copy of the homologous region (hr1) sequence in the Autographa californica multinucleocapsid polyhedrosis virus genome promotes hyperexpression of foreign genes. Biochemistry, 2004, 43(25): 8143-51.
69. Viswanathan P, Venkaiah B, Kumar MS, et al. The homologous region sequence (hr1) of Autographa californica multinucleocapsid polyhedrosis virus can enhance transcriptionfrom non-baculoviral promoters in mammalian cells. J Biol Chem, 2003, 278(52): 52564-71.
70. Hu YC, Tsai CT, Chang YJ, et al. Enhancement and prolongation of baculovirus-mediated expression in mammalian cells: focuses on strategic infection and feeding. Biotechnol Prog, 2003, 19(2): 373-9.
71. Merrihow RV, Clay WC, Condreay JP, et al. Chromosomal integration of transduced recombinant baculovirus DNA in mammalian cells. J Virol, 2001, 75(2): 903-9.
72. Palombo F, Monciotti A, Recchia A, et al. Site-specific integration in mammalian cells mediated by a new hybrid baculovirus-adeno-associated virus vector. J Virol, 1998, 72(6): 5025-34.
73. Fipaldini C, Bellei B, La M N. Expression of hepatitis C virus cDNA in human hepatoma cell line mediated by a hybrid baculovirus HCV vector. Virology, 1999, 255(2): 302-11.
74. Delaney WE 4th, Isom HC. Hepatitis B virus replication in human HepG2 cells mediated by hepatitis B virus recombinant baculovirus. Hepatology, 1998, 28(4): 1134-46.
75. Song SU, Boyce FM. Combination treatment for osteosarcoma with baculoviral vector mediated gene therapy (p53) and chemotherapy (adriamycin). Exp Mol Med, 2001, 33(1): 46-53.
76. Tani H, Limn CK. In vitro and in vivo gene delivery by recombinant baculoviruses. J Virol, 2003, 77(18): 9799-808.
77. Huser A, Rudolph M, Hofmann C. Incorporation of decay-accelerating factor into the baculovirus envelope generates complement-resistant gene transfer vectors. Nat Biotechnol, 2001, 19(5): 451-5.
78. Delaney WE 4th, Isom HC. Hepatitis B virus replication in human HepG2 cells mediated by hepatitis B virus recombinant baculovirus. Hepatology, 1998, 28(4): 1134-46.
79. Hofmann C, Huser A, Lehnert W, et al. Protection of baculovirus-vectors against complement-mediated inactivation by recombinant soluble complement receptor type 1. Biol Chem, 1999, (3): 393-5.
80. Lehtolainen P, Tyynela K, Kannasto J, et al. Baculoviruses exhibit restricted cell type specificity in rat brain: a comparison of baculovirus- and adenovirus-mediated intracerebral gene transfer in vivo. Gene Ther, 2002, 9(24): 1693-9.
81. Bilello JP, Delaney WE 4th, Boyce FM, et al. Transient disruption of intercellular junctions enables baculovirus entry into nondividing hepatocytes. J Virol, 2001, 75(20): 9857-71.
82. 顾健人, 曹雪涛. 基因治疗. 北京: 科学出版社, 2001, 2-22.
83. 曹翠平, 吴小锋. 昆虫杆状病毒应用于哺乳动物基因治疗的研究进展. 微生物学报, 2006, 46(4): 668-67.
84. 齐义鹏, 刘子夜, 朱应等. 杆状病毒介导对哺乳动物细胞的基因转移. 中国科学(C辑), 1998, 28(4): 295-301.
85. 程通, 许辰煜, 王颖彬等. 杆状病毒用于哺乳动物细胞快速高效表达外源基因的研究. 生物工程学报, 2003, 19(5): 581-586.
86. O'Reilly DR, Miler LK, Luckow VA. Baculovirus Expression Vectors: A Laboratory manual. lsted. New York: Oxford University Press, 1992.
87. Hu YC, Bentley WE, Edwards GH, et al. Chimeric infectious bursal disease virus-like particles expressed in insect cells and purified by immobilized metal affinity chromatography. Biotechnol Bioeng, 1999, 63(6): 721-9.
88. Ikonomou L, Schneider YJ, Agathos SN. Insect cell culture for industrial production of recombinant proteins. Appl Microbiol Biotechnol, 2003, 62(1): 1-20.
89. Ho Y, Lin PH, Liu CY, et al. Assembly of human severe acute respiratory syndrome coronavirus-like particles. Biochem Biophys Res Commun, 2004, 318(4): 833-8.
90. Mortola E, Roy P. Efficient assembly and release of SARS coronavirus-like particle by a heterologous expression system. FEBS Lett, 2004, 576(1-2): 174-8.
91. 尤蓓, 李彪, 陆盈等. 重组杆状病毒在哺乳动物细胞中的表达. 上海第二医科大学学报, 2004, 24(10): 806-810.
92. Carbonell LF, Klowden MJ, et al. Baculovirus-mediated expression of bacterial genes in dipteran and mammalian cells. J Virol, 1985, 56(1): 153-60.
93. Kost TA, Condreaay JP. Recombinant baculovirus as mammalian cell gene-deliveryvector. Trends Biotechnol, 2002, 20(4): 173-180.
94. Smith TJ, Piscatelli JJ, Andersen V, et al. Butyrate induces plasminogen activator inhibitor type 1 messenger RNA in cultured HepG2 cells. Hepatology, 1996, 23(4): 866-71.
95. Arts J, Lansink M, Grimbergen J, et al. Stimulation of tissue-type plasminogen activator gene expression by sodium butyrate and trichostatin A in human endothelial cells involves histone acetylation. Biochem J, 1995, 310 (1): 171-6.
96. Kramer OH, Gottlicher M, Heinzel T. Histone deacetylase as a therapeutic target. Trends Endocrinol Metab, 2001, 12(7): 294-300.
97. Crawford AM, Miller LK. Characterization of an early gene accelerating expression of late genes of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol, 1988, 62(8): 2773-81.
2. Maeda S, Kawai T, Obinata M, et al. Production of human alpha-interferon in silkworm using a baculovirus vector. Nature, 1985, 315(6020): 592-594.
3. Sriram S, Palhan VB, Gopinathan KP. Heterologous promoter recognition leading to high-level expression of cloned foreign genes in Bombyx mori cell lines and larvae. Gene, 1997, 190(1): 181-189.
4. Cha HJ, Dalal NG, Vakharia VN, et a1. Expression and purification of human interleukin-2 simplified as a fusion with green fluorescent protein in suspended sf-9 insect cells. Biotechnology, 1999, 69(1): 9-17.
5. 卢兹凡, 陈苏民, 陈南春等. 人 BMP-2 在昆虫杆状病毒表达系统中的表达及初步鉴定. 细胞与分子免疫学杂志, 1999, l5(3):170-172.
6. Vlak JM, Keus RJ. Baculovirus expression vector system for production of viral vaccines. Adv Biotechnol Processes, 1990, 14: 91-128.
7. Ailor E, Betenbaugh MJ. Modifying secretion and post-translational processing in insect cells. Curr Opin Biotechnol, 1999, 10(2): 42-145.
8. Joshi L, Davis TR, Mattu TS, et a1. Influence of baculovirus-host cell interactions on complex N-linked glycosylation of a recombinant human protein. Biotechnol Prog, 2000, 16(4): 650-656.
9. Dai X, Hajos JP, Joosten NN, et a1. Isolation of a Spodoptera exigua baculovirus recombinant with a 10.6 kbp genome deletion that retains biological activity. J Gen Virol, 2000, 81(10): 2545-54.
10. Sandoval C, Curtis H, Congote LF. Enhanced proliferative effects of a baculovirus-produced fusion protein of insulin-like growth factor and alpha(1)-proteinase inhibitor and improved anti-elastase activity of the inhibitor with glutamate at position 351. Protein Eng, 2002, 15(5): 413-418.
11. 郭君惠, 杜昌生, 彭建新等. 重组杆状病毒安全性及其评价. 中国生物防治, 2003, 19 (1): 40-43.
12. Kost TA, Condreay JP, Jarvis DL. Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nature Biotechnology, 2005, 23(5): 567-575.
13. Kitts PA, Ayres MD, Possee RD. Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acids Res, 1990, 18(19): 5667-5672.
14. Luckow VA, Lee SC, Barry GF, et al. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J Virol, 1993, 67(8): 4566-79.
15. Invitrogen life technologies instruction Manual. Guide to Baculovirus Expression Vector system (BEVS) and insect cell culture techniques.
16. DiFalco MR, Bakopanos E, Patricelli M, et al. The influence of various insect cell lines, p10 and polyhedrin promoters in the production of secreted insulin-like growth factor-interleukin-3 chimeras in the baculovirus expression system. J Biotechnol, 1997, 56(1): 49-56.
17. Zuidema D, Klinge-Roode EC, van Lent JW, et al. Construction and analysis of an Autographa californica nuclear polyhedrosis virus mutant lacking the polyhedral envelope. Virology, 1989, 173(1): 98-108.
18. Vialard J, Lalumiere M, Vernet T, et al. Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector containing the beta-galactosidase gene. J Virol, 1990, 64(1): 37-50.
19. Thiem S M, Miller LK. Differential gene expression mediated by late, very late and hybrid baculovirus promoters. Gene, 1990, 91(1): 87-94.
20. Vlak JM, Klinkenberg FA, Zaal KJ, et al. Functional studies on the p10 gene of Autographa californica nuclear polyhedrosis virus using a recombinant expressing a p10-beta-galactosidase fusion gene. J Gen Virol, 1988, 69(4): 765-776.
21. Hill-Perkins MS, Possee RD. A baculovirus expression vector derived from the basic protein promoter of Autographa californica nuclear polyhedrosis virus. J Gen Virol, 1990,71 (4): 971-6.
22. Liu YK, Chu CC, Wu TY. Baculovirus ETL promoter acts as a shuttle promoter between insect cells and mammalian cells. Acta Pharmacol Sin, 2006, 27(3): 321-7.
23. Daniela M, Andreas K, Dagmar KM. Baculovirus transactivator IE1 is functional in mammalian cells. J Gen Virol, 1997, 78(6): 1507-10.
24. Liu WJ, Chang YS, Wang CH, et al. Microarray and RT-PCR screening for white spot syndrome virus immediate-early genes in cycloheximide-treated shrimp. Virology, 2005, 334(2): 327-341.
25. Liu WJ, Chang YS, Wang AH, et al. White spot syndrome virus annexes a shrimp STAT to enhance expression of the immediate-early gene ie1. J Virol, 2007, 81(3): 1461-71.
26. 朱江, 吴祥甫. 昆虫杆状病毒表达系统研究进展及其应用展望. 蚕业科学, 2003, 29(2): 114-119.
27. 贾帅争. 国外医学分子生物学分册, 2000, 22(1): 49-52.
28. 陈茹. 中国进出境动植物检疫, 1997, 4: 36-38.
29. 王立良. 国外医学分子生物学分册, 1998, 20(5): 222-226.
30. Drings A, Jallet C, Chambert B, et al. Is there an advantage to including the nucleoprotein in a rabies glycoprotein subunit vaccine? Vaccine, 1999, 17(11-12): 1549-57.
31. Touze A, Dupuy C, Chabaud M, et al. Production of human papillomavirus type 45 virus-like particles in insect cells using a recombinant baculovirus. FEMS Microbiol Lett, 1996, 141(1): 111-6.
32. Wang YH, Davies AH, Jones IM. Expression and purification of glutathione S-transferase-tagged HIV-1 gp120: no evidence of an interaction with CD26. Virology, 1995, 208(1): 142-6.
33. Tobin GJ, Nagashima K, Gonda MA. Immunologic and ultrastructural characterization of HIV pseudovirions containing Gag and Env precursor proteins engineered in insect cells. Methods, 1996, 10(2): 208-218.
34. Crawford J, Wilkinson B, Vosnesensky A, et al. Baculovirus-derived hemagglutinin vaccines protect against lethal influenza infections by avian H5 and H7 subtypes. Vaccine, 1999, 17(18): 2265-74.
35. Meyer RF, Babcock GD, Newman JF, et al. Baculovirus expressed 2C of foot-and-mouth disease virus has the potential for differentiating convalescent from vaccinated animals. J Virol Methods, 1997, 65(1): 33-43.
36. Shi YP, Das P. Development, expression and murine testing of a multistage Plasmodium falciparum malaria vaccine candidate. Vaccine, 2000, 18(25): 2902-14.
37. Hota-Mitchell S, Siddiqui AA, Dekaban GA, et al. Protection against Schistosoma mansoni infection with a recombinant baculovirus-expressed subunit of calpain. Vaccine, 1997, 15(15): 1631-40.
38. Bae JE, Schurig GG, Toth TE. Mice immune responses to Brucella abortus heat shock proteins. Use of baculovirus recombinant-expressing whole insect cells, purified Brucella abortus recombinant proteins, and a vaccinia virus recombinant as immunogens. Vet Microbiol, 2002, 88(2): 189-202.
39. 许辰煜, 程通, 卢五迅等. 杆状病毒对不同哺乳动物细胞基因转移及表达效率的研究. 生物工程学报, 2004, 20(1): 73-77.
40. Boyce FM, Bucher NL. Baculovirus mediated gene transfer into mammalian cells. Proc Natl Acad Sci USA, 1996, 93(6): 2348-52.
41. Condreay JP, Witherspoon SM, Clay WC, et al. Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc Natl Acad Sci USA, 1999, 96: 127-32.
42. Airenne KJ, Hiltunen MO, Turunen MP, et al. Baculovirus-mediated periadventitial gene transfer to rabbit carotid artery. Gene Ther, 2000, 7(17): 1499-504.
43. Liu X, Li K, Song J, et al. Efficient and stable gene expression in rabbit intervertebral disc cells transduced with a recombinant baculovirus vector. Spine, 2006, 31(7): 732-735.
44. Forstova J, Krauzewicz N, Sandig V, et al. Polyoma virus pseudocapsids as efficient carriers of heterologous DNA into mammalian cells. Hum Gene Ther, 1995, 6(3): 297-306.
45. Lehtolainen P, Tyynela K, Kannasto J, et al. Baculoviruses exhibit restricted cell type specificity in rat brain: a comparison of baculovirus- and adenovirus-mediated intracerebral gene transfer in vivo. Gene Ther, 2002, 9(24): 1693-9.
46. Sandig V, Hofmann C, Steinert S, et al. Gene transfer into hepatocytes and human liver tissue by baculovirus vectors. Hum Gene Ther, 1996, 7(16): 1937-1945.
47. Hofmann C, Strauss M. Baculovirus-mediated gene transfer in the presence of human serum or blood facilitated by inhibition of the complement system. Gene Ther, 1998, 5(4): 531-536.
48. Pieroni L, Maione D, La Monica N. In vivo gene transfer in mouse skeletal muscle mediated by baculovirus vectors. Hum Gene Ther, 2001, 12(8): 871-881.
49. Sarkis C, Serguera C, Petres S, et al. Efficient transduction of neural cells in vitro and in vivo by a baculovirus-derived vector. Proc Natl Acad Sci USA, 2000, 97: 14638-14643
50. Volkman LE, Goldsmit PA. In Vitro Survey of Autographa californica Nuclear Polyhedrosis Virus Interaction with Nontarget Vertebrate Host Cells. Appl Environ Microbiol, 1983, 45(3): 1085-1093.
51. Hofmann C, Sandig V, Jennings G, et al. Efficient gene transfer into human hepatocytes by baculovirus vectors. Proc Natl Acad Sci USA, 1995, 92(22): 10099-103.
52. Boyce FM, Butcher NL. Baculovirus-mediated gene transfer into mammalian cells. Proc Natl Acad Sci USA, 1996, 93(6): 2348-52.
53. Shoji I, Aizaki H, Tani H, et al. Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors. J Gen Virol, 1997, 78 (10): 2657-64
54. Ma L, Tamarina N, Wang Y, et al. Baculovirus-mediated gene transfer into pancreatic islet cells. Diabetes, 2000, 49(12): 1986-91.
55. Wagle M, Jesuthasan S. Baculovirus-mediated gene expression in zebrafish. Mar Biotechnol (NY), 2003, 5(1): 58-63.
56. Cheng T, Xu CY, Wang YB, et al. A rapid and efficient method to express target genes in mammalian cells by baculovirus. World J Gastroenterol, 2004, 10(11): 1612-8.
57. Dee KU, Shuler ML. Optimization of an assay for baculovirus titer and design of regimens for the synchronous infection of insect cells. Biotechnol Prog, 1997, 13(1): 14-24.
58. 梁昌墉, 胡志红, 陈新文. 杆状病毒作为基因治疗载体的研究进展. 生物化学与生物物理进展, 2002, 29 (6): 860-862.
59. Duisit G, Saleun S, Douthe S, et al. Baculovirus vector requires electrostatic interactions including heparan sulfate for efficient gene transfer in mammalian cells. J Gene Med, 1999, 1(2): 93-102.
60. Van Loo ND, Fortunati E, Ehlert E, et al. Baculovirus infection of nondividing mammalian cells: mechanisms of entry and nuclear transport of capsids. J Virol, 2001, 75(2): 961-70.
61. Hofmann C, Hermanns W, et al. Demonstration of cells of the mononuclear phagocyte system of the cat using enzyme and immunohistochemistry methods. Berl Munch Tierarztl Wochenschr, 1995, 108(3): 81-7.
62. Tani H, Nishijima M, Ushijima H, et al. Characterization of cellsurface determinants important for baculovirus infection. Virology, 2001, 279(1): 343-353.
63. Bilelo JP, Cable EE, Myers RL, et al. Role of paracellular junction complexes in baculovirus-mediated gene transfer to nondividing rat hepatocytes. Gene Ther, 2003, 10(9): 733-749.
64. Barsoum J, Brown R, McKee M, et al. Efficient transduction of mammalian cells by a recombinant baculovirus having the vesicular stomatitis virus G glycoprotein. Hum Gene Ther, 1997, 8(17): 2011-8.
65. Kukkonen SP, Airenne KJ, Marjomaki V, et al. Baculovirus capsid display: a novel tool for transduction imaging. Mol Ther, 2003, 8(5): 853-62.
66. Ho YC, Chung YC, Hwang SM, et al. Transgene expression and differentiation of baculovirus-transduced human mesenchymal stem cells. J Gene Med, 2005, 7(7): 860-8.
67. Lo HR, Chou CC, Wu TY, et al. Novel baculovirus DNA elements strongly stimulate activities of exogenous and endogenous promoters. J Biol Chem, 2002, 277(7): 5256-64.
68. Venkaiah B, Viswanathan P, Habib S, et al. An additional copy of the homologous region (hr1) sequence in the Autographa californica multinucleocapsid polyhedrosis virus genome promotes hyperexpression of foreign genes. Biochemistry, 2004, 43(25): 8143-51.
69. Viswanathan P, Venkaiah B, Kumar MS, et al. The homologous region sequence (hr1) of Autographa californica multinucleocapsid polyhedrosis virus can enhance transcriptionfrom non-baculoviral promoters in mammalian cells. J Biol Chem, 2003, 278(52): 52564-71.
70. Hu YC, Tsai CT, Chang YJ, et al. Enhancement and prolongation of baculovirus-mediated expression in mammalian cells: focuses on strategic infection and feeding. Biotechnol Prog, 2003, 19(2): 373-9.
71. Merrihow RV, Clay WC, Condreay JP, et al. Chromosomal integration of transduced recombinant baculovirus DNA in mammalian cells. J Virol, 2001, 75(2): 903-9.
72. Palombo F, Monciotti A, Recchia A, et al. Site-specific integration in mammalian cells mediated by a new hybrid baculovirus-adeno-associated virus vector. J Virol, 1998, 72(6): 5025-34.
73. Fipaldini C, Bellei B, La M N. Expression of hepatitis C virus cDNA in human hepatoma cell line mediated by a hybrid baculovirus HCV vector. Virology, 1999, 255(2): 302-11.
74. Delaney WE 4th, Isom HC. Hepatitis B virus replication in human HepG2 cells mediated by hepatitis B virus recombinant baculovirus. Hepatology, 1998, 28(4): 1134-46.
75. Song SU, Boyce FM. Combination treatment for osteosarcoma with baculoviral vector mediated gene therapy (p53) and chemotherapy (adriamycin). Exp Mol Med, 2001, 33(1): 46-53.
76. Tani H, Limn CK. In vitro and in vivo gene delivery by recombinant baculoviruses. J Virol, 2003, 77(18): 9799-808.
77. Huser A, Rudolph M, Hofmann C. Incorporation of decay-accelerating factor into the baculovirus envelope generates complement-resistant gene transfer vectors. Nat Biotechnol, 2001, 19(5): 451-5.
78. Delaney WE 4th, Isom HC. Hepatitis B virus replication in human HepG2 cells mediated by hepatitis B virus recombinant baculovirus. Hepatology, 1998, 28(4): 1134-46.
79. Hofmann C, Huser A, Lehnert W, et al. Protection of baculovirus-vectors against complement-mediated inactivation by recombinant soluble complement receptor type 1. Biol Chem, 1999, (3): 393-5.
80. Lehtolainen P, Tyynela K, Kannasto J, et al. Baculoviruses exhibit restricted cell type specificity in rat brain: a comparison of baculovirus- and adenovirus-mediated intracerebral gene transfer in vivo. Gene Ther, 2002, 9(24): 1693-9.
81. Bilello JP, Delaney WE 4th, Boyce FM, et al. Transient disruption of intercellular junctions enables baculovirus entry into nondividing hepatocytes. J Virol, 2001, 75(20): 9857-71.
82. 顾健人, 曹雪涛. 基因治疗. 北京: 科学出版社, 2001, 2-22.
83. 曹翠平, 吴小锋. 昆虫杆状病毒应用于哺乳动物基因治疗的研究进展. 微生物学报, 2006, 46(4): 668-67.
84. 齐义鹏, 刘子夜, 朱应等. 杆状病毒介导对哺乳动物细胞的基因转移. 中国科学(C辑), 1998, 28(4): 295-301.
85. 程通, 许辰煜, 王颖彬等. 杆状病毒用于哺乳动物细胞快速高效表达外源基因的研究. 生物工程学报, 2003, 19(5): 581-586.
86. O'Reilly DR, Miler LK, Luckow VA. Baculovirus Expression Vectors: A Laboratory manual. lsted. New York: Oxford University Press, 1992.
87. Hu YC, Bentley WE, Edwards GH, et al. Chimeric infectious bursal disease virus-like particles expressed in insect cells and purified by immobilized metal affinity chromatography. Biotechnol Bioeng, 1999, 63(6): 721-9.
88. Ikonomou L, Schneider YJ, Agathos SN. Insect cell culture for industrial production of recombinant proteins. Appl Microbiol Biotechnol, 2003, 62(1): 1-20.
89. Ho Y, Lin PH, Liu CY, et al. Assembly of human severe acute respiratory syndrome coronavirus-like particles. Biochem Biophys Res Commun, 2004, 318(4): 833-8.
90. Mortola E, Roy P. Efficient assembly and release of SARS coronavirus-like particle by a heterologous expression system. FEBS Lett, 2004, 576(1-2): 174-8.
91. 尤蓓, 李彪, 陆盈等. 重组杆状病毒在哺乳动物细胞中的表达. 上海第二医科大学学报, 2004, 24(10): 806-810.
92. Carbonell LF, Klowden MJ, et al. Baculovirus-mediated expression of bacterial genes in dipteran and mammalian cells. J Virol, 1985, 56(1): 153-60.
93. Kost TA, Condreaay JP. Recombinant baculovirus as mammalian cell gene-deliveryvector. Trends Biotechnol, 2002, 20(4): 173-180.
94. Smith TJ, Piscatelli JJ, Andersen V, et al. Butyrate induces plasminogen activator inhibitor type 1 messenger RNA in cultured HepG2 cells. Hepatology, 1996, 23(4): 866-71.
95. Arts J, Lansink M, Grimbergen J, et al. Stimulation of tissue-type plasminogen activator gene expression by sodium butyrate and trichostatin A in human endothelial cells involves histone acetylation. Biochem J, 1995, 310 (1): 171-6.
96. Kramer OH, Gottlicher M, Heinzel T. Histone deacetylase as a therapeutic target. Trends Endocrinol Metab, 2001, 12(7): 294-300.
97. Crawford AM, Miller LK. Characterization of an early gene accelerating expression of late genes of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol, 1988, 62(8): 2773-81.