石斛属药用植物内生真菌多样性研究
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摘要
石斛属植物为附生性兰科植物,多种具有药用价值。自然条件下所有石斛属植物均与内生真菌共生,内生真菌在石斛属植物整个生活史中扮演着重要角色。针对石斛属植物内生真菌的研究得到了大量学者的重视,但是当前国内外针对石斛属植物内生真菌多样性的研究少有报道。本研究以石斛属植物为研究对象,通过形态学和分子生物学鉴定对24种石斛属药用植物中分离到的542株内生真菌进行了多样性研究。
鉴于大量内生真菌不产孢无法通过形态学鉴定的现实,我们首先对内生真菌产孢诱导的方法进行了筛选。本研究选用美登木、金线莲两种药用植物来源的74株常规条件下不产孢的内生真菌进行了产孢诱导实验。结果表明低温诱导对内生真菌产孢诱导效果较为理想,因而在我们对石斛属药用植物内生真菌不产孢菌株采用低温诱导法诱导产孢。在该实验过程中,我们从金线莲内生真菌中发现一个Hansfordia的新种,定名为Hansfordia pallens。
542株石斛属药用植物内生真菌中249株可产孢,占总菌株数的46%。通过形态学观察249株石斛内生真菌鉴定到21个属,其中148株鉴定到种,分别属于51个种,其中发现3个中国新纪录种(Acremonium sordidulum,Periconiella cestri,Hyalodendron lignicola ).Alternaria,Cladosporium,Aureobasidium,Chaetophoma,Periconiella,Nigrospora,Hyalodendron,Thielavia,Pleurophragmium,Melanconium,Glomerularia,Sirodesmium,Ckaetomella,Streptomyces 14个属系首次从石斛属植物中发现。
虽然我们对石斛属药用植物不产孢的内生真菌进行了产孢诱导,但仍有293株不产孢,无法通过传统形态学方法进行鉴定。对于不产孢的菌株,我们采用分子生物学手段来对其进行鉴定。根据菌落培养特征和显微观察特征,将不产孢菌株划分为30个形态型,从30个形态型中选取148个代表菌株进行分子生物学鉴定。对148株内生真菌的ITS片段进行PCR扩增测序,将获得的ITS序列与参考菌种一起进行系统发育分析,结合序列碱基相似性比对来确定石斛属药用植物内生真菌的分类地位。通过分子生物学鉴定,148株代表菌株中8株鉴定到种,分别属于8个不同种;128株鉴定到属,分别属于28个属;10株鉴定到科,分别属于5个科;2株放到目。形态学鉴定的28个属中,Arthrinium,Chaetomium,Colletotrichum,Cercophora,Phomopsis,Nectria,Cosmospora,Bionectria,Nemania,Pleospora,Leptosphaerulina,Lewia,Paraconiothyrium,Ampelomyces,Davidiella,Lasiodiplodia,Fusicoccum,Botryosphaeria,Pezicula,Hypocrea20个属系首次从石斛属植物中发现。形态学鉴定结果结合分子生物学鉴定结果表明,石斛属药用植物内生真菌种类非常丰富,不同种石斛属药用植物的内生真菌在数量上和种类上差别较大,多样性丰富程度不同。
Dendrobium is an adnascent plant of the orchid family, which includes many species with medicinal value. Endophytic fungi can be found in all kinds of Dendrobium under natural conditions and it plays an important role in the life history of Dendrobium. More attentions have been paid to the endophytic fungi of Dendrobium. However, very few studies have investigated the diversity of endophytic fungi of Dendrobium. The diversity of 542 isolates of endophytic fungi from 24 species medicinal Dendrobium plants was systematically studied in this thesis.
Some methods in promoting sporulation were investigated using 77 isolates of endophytic fungi in Maytenus and Anoectochilus for the research material. Low temperature treatment was found to be the best method for promoting sporulation. So this method was used to promote sporulation of isolates in Dendrobium. A new species, Hansfordia pallens, isolated from living leaves of Anoectochilus roxburghii was found in this work.
A total of 249 isolates sporulated of medicinal Dendrobium were identified into 21 genus based on morphological characteristics. 148 isolates were identified to species, and they were subordinated to 51 species including 3 new records Acremonium sordidulum, Periconiella cestri, Hyalodendron lignicola. Among them, 14 genus were found in Dendrobium for the first time, Alternaria, Cladosporium, Aureobasidium, Chaetophoma, Periconiella, Nigrospora, Hyalodendron, Thielavial, Pleurophragmium, Melanconium, Glomerularia, Sirodesmium, Chaetomella, Streptomyces in Dendrobium. Fusarium and Acremonium were found to be predominance populations of Dendrobium endophtic fungi.
Approach 54% isolates could not produce any spore, although low temperature treatment was done for promoting sporulation. These isolates were divided to 30 morphotypes based on similar cultural characters. Our method was to identify 148 strains of these morphotypes based on phylogenetic analysis of ITS and 5.8S gene sequences, as well as sequence similarity comparison. The results showed that 8 strains were identified to species level, 128 strains to 28 generic level, 10 strains to family level, and 2 strains to order level. Among them, 21 genus were found in Dendrobium for the first time, Arthrinium, Chaetomium, Colletotrichum, Cercophora, Phomopsis, Nectria, Cosmospora, Bionectria, Nemania, Pleospora, Leptosphaerulina, Lewia, Paraconiothyrium, Ampelomyces, Davidiella, Lasiodiplodia, Fusicoccum, Botryosphaeria, Pezicula, Hypocrea. The identification result based on both morphological and molecular characters shows that the kind and amount of endophytic fungi are different according to different species of plants in medicinal Dendrobium.
鉴于大量内生真菌不产孢无法通过形态学鉴定的现实,我们首先对内生真菌产孢诱导的方法进行了筛选。本研究选用美登木、金线莲两种药用植物来源的74株常规条件下不产孢的内生真菌进行了产孢诱导实验。结果表明低温诱导对内生真菌产孢诱导效果较为理想,因而在我们对石斛属药用植物内生真菌不产孢菌株采用低温诱导法诱导产孢。在该实验过程中,我们从金线莲内生真菌中发现一个Hansfordia的新种,定名为Hansfordia pallens。
542株石斛属药用植物内生真菌中249株可产孢,占总菌株数的46%。通过形态学观察249株石斛内生真菌鉴定到21个属,其中148株鉴定到种,分别属于51个种,其中发现3个中国新纪录种(Acremonium sordidulum,Periconiella cestri,Hyalodendron lignicola ).Alternaria,Cladosporium,Aureobasidium,Chaetophoma,Periconiella,Nigrospora,Hyalodendron,Thielavia,Pleurophragmium,Melanconium,Glomerularia,Sirodesmium,Ckaetomella,Streptomyces 14个属系首次从石斛属植物中发现。
虽然我们对石斛属药用植物不产孢的内生真菌进行了产孢诱导,但仍有293株不产孢,无法通过传统形态学方法进行鉴定。对于不产孢的菌株,我们采用分子生物学手段来对其进行鉴定。根据菌落培养特征和显微观察特征,将不产孢菌株划分为30个形态型,从30个形态型中选取148个代表菌株进行分子生物学鉴定。对148株内生真菌的ITS片段进行PCR扩增测序,将获得的ITS序列与参考菌种一起进行系统发育分析,结合序列碱基相似性比对来确定石斛属药用植物内生真菌的分类地位。通过分子生物学鉴定,148株代表菌株中8株鉴定到种,分别属于8个不同种;128株鉴定到属,分别属于28个属;10株鉴定到科,分别属于5个科;2株放到目。形态学鉴定的28个属中,Arthrinium,Chaetomium,Colletotrichum,Cercophora,Phomopsis,Nectria,Cosmospora,Bionectria,Nemania,Pleospora,Leptosphaerulina,Lewia,Paraconiothyrium,Ampelomyces,Davidiella,Lasiodiplodia,Fusicoccum,Botryosphaeria,Pezicula,Hypocrea20个属系首次从石斛属植物中发现。形态学鉴定结果结合分子生物学鉴定结果表明,石斛属药用植物内生真菌种类非常丰富,不同种石斛属药用植物的内生真菌在数量上和种类上差别较大,多样性丰富程度不同。
Dendrobium is an adnascent plant of the orchid family, which includes many species with medicinal value. Endophytic fungi can be found in all kinds of Dendrobium under natural conditions and it plays an important role in the life history of Dendrobium. More attentions have been paid to the endophytic fungi of Dendrobium. However, very few studies have investigated the diversity of endophytic fungi of Dendrobium. The diversity of 542 isolates of endophytic fungi from 24 species medicinal Dendrobium plants was systematically studied in this thesis.
Some methods in promoting sporulation were investigated using 77 isolates of endophytic fungi in Maytenus and Anoectochilus for the research material. Low temperature treatment was found to be the best method for promoting sporulation. So this method was used to promote sporulation of isolates in Dendrobium. A new species, Hansfordia pallens, isolated from living leaves of Anoectochilus roxburghii was found in this work.
A total of 249 isolates sporulated of medicinal Dendrobium were identified into 21 genus based on morphological characteristics. 148 isolates were identified to species, and they were subordinated to 51 species including 3 new records Acremonium sordidulum, Periconiella cestri, Hyalodendron lignicola. Among them, 14 genus were found in Dendrobium for the first time, Alternaria, Cladosporium, Aureobasidium, Chaetophoma, Periconiella, Nigrospora, Hyalodendron, Thielavial, Pleurophragmium, Melanconium, Glomerularia, Sirodesmium, Chaetomella, Streptomyces in Dendrobium. Fusarium and Acremonium were found to be predominance populations of Dendrobium endophtic fungi.
Approach 54% isolates could not produce any spore, although low temperature treatment was done for promoting sporulation. These isolates were divided to 30 morphotypes based on similar cultural characters. Our method was to identify 148 strains of these morphotypes based on phylogenetic analysis of ITS and 5.8S gene sequences, as well as sequence similarity comparison. The results showed that 8 strains were identified to species level, 128 strains to 28 generic level, 10 strains to family level, and 2 strains to order level. Among them, 21 genus were found in Dendrobium for the first time, Arthrinium, Chaetomium, Colletotrichum, Cercophora, Phomopsis, Nectria, Cosmospora, Bionectria, Nemania, Pleospora, Leptosphaerulina, Lewia, Paraconiothyrium, Ampelomyces, Davidiella, Lasiodiplodia, Fusicoccum, Botryosphaeria, Pezicula, Hypocrea. The identification result based on both morphological and molecular characters shows that the kind and amount of endophytic fungi are different according to different species of plants in medicinal Dendrobium.
引文
[1]De Bary A.Morphologe and Physiologic der Pilze,Flechten and Myxomyceten[J].Engelmann,Leipzig,1866,1-316.
[2]Carroll G C.The biology of endophytism in plants with particular reference to woody plants[A].In:Fokkema N J,van den Heuvel J.Microbiology of the Phyllosphere[M].Cambridge,U.K:Cambridge University Press,1986:205-222.
[3]Petrini O.Fungal endophytes of tree leaves[J].New York Springerverlag,1991,179-197.
[4]郭良栋.内生真菌研究进展[J].菌物系统,2001,20(1):148-152.
[5]官珊,钟国华,孙之潭,胡美英.植物内生真菌的研究进展[J].仲恺农业技术学院学报,2005,18(1):61-66.
[6]Petrini.Taxonomy of endophytic fungi of aerial plant tissues[A].In N.J.Fokkema and J.van denHeuvel.Microbiology of the Phyllosphere[M].England:Cambridge University Press,1986:175-187.
[7]Guo L D,Hyde K D,Liew E C Y.Identification of endophytic fungi from Livistona chinensis based on morphology and Rdna sequences[J].New phytol,2000,147:617-630.
[8]Sinclair J B,Cerkauskas.Latent infection vs.endophytic colonization by fungi[A].In:Redlin S C,Carris L Meds.Endophytic Fungi in Grasses and Woody Plants:Systematics,Ecology,and Evolution[M].St.Paul,Minnesota:APS Press,1996:3-29.
[9]Zheng R,Jiang H.Rhizomucor endophyticus sp.nov.,an endophytic zygomycetes from higher plants[J].Mycotaxon,1995,56:455-466.
[10]Siegel M R,Johnson M C,Varney D R.A fungal endophytic in tall fescue:ncidence and dissemination[J].Phytopathology,1984,74:932-937.
[11]Ananda K,Sridhar K R.Diversity of endophytic funni in the toots of mannrove species on the west of India[J].Can J Microbiol,2002,48(10):871-878.
[12]徐锦堂,郭顺星.供给天麻种子萌发营养的真菌-紫萁小菇[J].真菌学报,1989,8(3):221-226.
[13]郭顺星,徐锦堂.密环菌隔膜发育的超微结构研究[J].中国医学科学院学报,1991, 13(1):46-49.
[14]郭顺星,范黎,曹文芩,陈晓梅.菌根真菌一新种-石斛小姑[J].菌物系统,1999,18(2):141-144.
[15]郭顺星,陈晓梅,于雪梅,范黎.金线莲菌根真菌的分离及其生物活性研究[J].中国药学杂志,2000,35(7):443-445.
[16]Strobel G.A proposed new taxon for a bulbilliferous hyphomycetes associated with pacific yew(Taxus brevifolia)[J].Mycotaxon,1993,XLV Ⅱ:(April-June):71-80.
[17]郭波,李海燕,张玲琪.一种产长春新碱内生真菌的分离[J].云南大学学报(自然科学版),1998,20(3):214-215.
[18]KUMAR D,SIVA Sundara,CHEUNG Honyeung.In vitro studies of endophytic funni from Tripterygium wilfordii with anti-proliferative acrivity on human peripheral blood mononnuclear cells[J].Journal of Ethnopharmacology,2004,94(2-3):295-300.
[19]Petrini O,Fisher P J.A comparative study of fungal endophytes in xylem and whole stern of pinus sylvestris and Fagus sylvatica[J].Trans Br Mycol Soc,1988,91:233-238.
[20]Toti L,Viret O,Chapela I H,Petrini O.Differential attachment by the conidia of the endophyte Discula umbrinella(Berk and Br.) Morelet to host and non-host surface[J].New Phytol,1992,121:469-475.
[21]Petrini O.Ecological and physiological aspects of host specificity in endophytic[A].In:Redlin S C,Carris L Meds.Endophytic Fungi in Grasses and Woody Plants:Systermatics,Ecology,and Evolution[M].St Paul,Minnesora:APS Press,1996,87-100.
[22]Fisher P J,Petrini O,Sutton B C.A comparative study of fungal endophytes in leaves,xylem and bark of Eucalyptus nitens in Australia and England[J].Sydowia,1993,45:338-345.
[23]范黎,郭顺星.兰科植物菌根真菌的研究进展[J].微生物学通报,1998,25(4):227-231.
[24]Peterson R L,Currah R S.Synthesis of mycorrhizae between protocorms of Goodyera repens(Orchidaceae) and Ceratobasi di um cereale[J].Can J Bot,1990,68:1117-1125.
[25]Rasmussen H N.Terrestrial orchids from seed to mycotrophic plant[M].New York:the press syndicate of the University of Cambridge,1995,1-237.
[26]范黎,郭顺星,肖培根.十九种兰科植物的内生担子菌[J].热带作物学报,1998,19(4):77-82.
[27]陈瑞蕊,林先贵,施亚琴.兰科菌根的研究进展[J].应用与环境生物学报,2003,9(1):97-101.
[28]郭顺星,曲文苏,高微微.铁皮石斛及金钗石斛菌根真菌的分离及其生物活性测定[J].中国中药杂志,2000,25(6):338-341.
[29]王利娟,贺新生.植物内生真菌分离培养的研究方法[J].微生物学杂志,2006,26(4):55-60.
[30]Masahide Yamato,Takahiro Yagame,Akira Suzuki,Koji Iwase.Isolation and identification of mycorrhizal fungi associating with an achlorophyllous plant,Epipogium roseum(Orchidaceae)[J].Mycoscience,2005,46:73-77.
[31]刘润进,李晓林.丛枝菌根及其应用[J].北京:科学出版,2000:1-186.
[32]杨显志,邵华,周成,宣群,杨春热,张玲琪.丛枝菌根研究及应用[J].云南农业 科技,2001,4:38-41.
[33]Harley J L,Smith S E.Mycorrhizal Symbiosis[M].London19:Academic Press,1983:1-483.
[34]Jeffrey K S,Polishook J D,White J F.Endophytic Fungi.In Gregory M.Mueller,Gerald F.Bills,Mercedes S.Foster.Biodiversity of Fungi,Inventoryan d Monitoring Methode[M].NewYork:Elsevier Academic Press,2004:241-270.
[35]弓明饮,陈应龙,仲崇禄.菌根研究及应用[M].北京:中国林业出版社,1997.
[36]Schulz B,Wanke U.Endophytes from erbaceous plants and shrubs:effectivness of surface-sterilization methods[J].Mycological Research,1993,97:1447-1450.
[37]张纪忠.微生物分类学[M].上海:复旦大学出版社,1990,341-365.
[38]Bills G F,Polishook J D.Microfungi from Carpinus careliana[J].Canadian Journal of Botany,1991,69:1477-1482.
[39]Bills G F,Polishook J D.Recovery of endophytic fungi from Chamaccyparis thyoides[J].Sydowia,1992,44:1-12.
[40]范黎,郭顺星,徐锦堂.我国部分兰科植物菌根的内生真菌种类研究[J].山西大学学报,1998,21(2):169-177.
[41]Olinto L P,Christtianno L R,Arnaldo C B,Kiyoshi M,Maria C M K.Epulorhiza epiphytica sp.nov.isolated from mycorrhizal roots of epiphytic orchids in Brazil[J].Mycoscience,2003,44:153-155.
[42]Stewart S,Kane M.Symbiotic seed germination of Habenaria macroceratitis (Orchidaceae),a rare Florida terrestrial orchid[J].Plant Cell,Tissue and Organ Culture,2006,82:159-167.
[43]张美庆,王幼珊.VA真菌的分类鉴定[J].华北农学报,1989(4):115-120.
[44]杨晓红,孙中海,邵菊芳,仝瑞建.丛枝菌根真菌培养方法研究进展[J].菌物学报,2004,23(3):444-456.
[45]Biermann B,Linderman R G.Use of vesicular-arbuscular mycorrhizal roots,intraradical vesicles and extraradical vesicles as inoculum[J].New Phytologist,1983,95:97-105.
[46]Diop T A,Plenchette C,Strullu D G.Dual axenic culture of sheared-root inocula of vesicular—arbuscular mycorrhizal fungi associated with tomato roots[J].Mycorrhiza,1994,5:17-22.
[47]Sylvia D M,Schenck N C.Application of superphosphate to mycorrhizal plants stimulates sporulation of phosphorus-tolerant vesicular-arbuscular mycorrhizai fungi[J].New Phytol,1983,95:655-661.
[48]Gilmore A E.Phytomycetous mycorrhizal organisms collected by open-pot culture methods[J].Hilgardia,1968.39:87-105.
[49]刘润进,李晓林.丛枝菌根及其应用[M].北京:科学出版社,2000,1-186.
[50]沈廷厚,万水林.柑桔VA菌根真菌繁殖及接种技术的应用研究[J].江西农业学报,1994,6(1):25-30.
[51]Ames R N,Linderman R G.The growth of easter lily(Lilium longiflorum) as influenced by vesicular-arbuscular mycorrhizal fungi,Fusarium oxysporum,and fertility level[J].Can J Bot,1978,56:2773-2780.
[52]Sylvia D M,Jarstfer A G.Production of inoculum and inoculation with arbuscular mycorrhizal fungi[A].In:Robson A D,Abbott L K,Malajczuk N.Management of Mycorrhizas in Agriculture,Horticulture and Forestry[M].New York:Kluwer,1994:1-248.
[53]Mosse B.The establishment of vesicular-arbuscular mycorrhiza under asetic conditions [J].J Gen Microbiol,1962,27:509-520.
[54]Crush J R,Hay M J.A technique for growing mycorrhizal clover in solution culture[J].N Z Journal of Agricultura Research,1981,24:371-372.
[55]陈应龙,弓明钦,王凤珍,陈羽.VA菌根菌剂的生产及其接种潜力的测定[J].热带亚热带土壤科学,1997,6(2):113-120.
[56]Elmes R P,Mosse B.Vesicular arbuscular endomycorrhizal inoculum production.11.Experiments with maize(Zea mays) and other hosts in nutrient flow culture[J].Can J Bot,1984,62:1531-1536.
[57]Hung V G,Sylvia D M.Production of vesicular—arbuscular mycnrrhizal fungus inoculum in aeroponic culture[J].Applied and Enviromental Microbiology,1988,54(2):353-357.
[58]Carruthers S.Aeroponics system review[J].Prac Hydropon July/August,1992,18-21.
[59]Jarstfer A G,Sylvia D M.Aeroponic culture of VAM fungi[A].In:Varma A,Hock B.Mycorrhiza-Structure,Function,Molecular Biology and Biotechnology[M].Tokyo:Springer-Verlag,1995:1-747.
[60]Mosse B,Hepper C M.Vesicular-arbuscular mycorrhizal infections in root organ cultures [J].Physiol Plant Pathol,1975,5:215-223.
[61]Redecker D,Thierfellfer H,Werner D.A new cultivation system for arbuscular mycorrhizal fungi on glass beads[J].Angewante Botanik,1995,69(5):189-191.
[62]陈保东,冯固,李晓林,崔建宇.玻璃珠分室培养AMF方法的建立[J].菌物系统,19(2):2000,212-216.
[63]Mosse B,Hepper C M.Vesicular-arbuscular mycorrhizai infections in root organ cultures [J].Physiol Plant Pathol,1975,5:215-223.
[64]Wideman M A,Watru d L S.Sporulation of Gigaspora margarita on root culture tomato [J].Can J Microbiol,1984,30:642-646..
[65]Janardhanan K K,Gupata M L,Husain A.Dual axenic culture of sheared-root inocula of vesicular-arbuscular mycorrhizal fungi associated with tomato roots[J].Current Science,1990,59(10):509-513.
[66]赵志鹏,郭秀珍.纯种VA菌根菌剂的制备及其在泡桐试管苗上的应用[J].微生物学报,1991,31(1):32-35.
[67]彭生斌,沈崇尧.用三叶草离体根建立VA菌根[J].微生物学报,1990,30(5):375-379.
[68]Mugnier J,Mosse B.Vesicular-arbuscular mycorrhizal infection in transformed Ri T-DNA roots grown axenically[J].Phytopathology,1987,77(7):1045-1050.
[69]Becard G,Fortin J A.Early events of vesicular-arbuscular mycorrhizal formation on Ri T-DNA transformed roots[J].New Phytol,1988,108:211-218.
[70]Chabot S,Becard G,Piche Y.Life cycle of Glomus intraradices in root organ culture[J].Mycologia,1992,84:315-321.
[71]李晓林,冯固.丛枝菌根生态生理[M].北京:华文出版社,2001,1-358.
[72]毕银丽,汪洪钢,李晓林.丛枝菌根的双重培养方法及其菌丝际的建立[J].菌物系统,2000,19(4):517-521.
[73]Fortin J A,St-Arnaud M,Hamel C,Chavarie C,Jolicoeur M.Aseptic in vitro endomycorrhizal spore mass production[P].US Patent Number:5554530,1996-10-09:
[74]Burggraaf A J P,Beringer J E.Absence of Nuclea DNA Synthesis in Vesicular-Arbuscular Mycorrhizal Fungi during In vitro Development[J].New Phytologist,1989.111(1),25-33.
[75]Becard G,Pfeffer P E.Status of nuclear division in arbuscular mycorrhizal fungi during in vitro development[J].Protoplasma,1993,194:62-68.
[76]Siqueira J O.Cultura axenicae monoxenica dos fungos microrrizicos vesiculo-arbusculares. In:Reuniao Brasileira sobre[J].Micorrizas,1987,44-70.
[77]Barea J M.Importance of hormones and root exudates in mycorrhiza phenomena[A].In:Gianinazzi-Pearson V,Gianinazzi S.Physiological and Genetic Aspects of Mycorrhizae [M].Paris:INRA,1986:1-832.
[78]Thompson J.Soil sterilization methods to show VA-mycorrhizae add P and Zn nutrition of wheat in vertisols[J].Soil Biol.Biochem,1990,26:1133-1143.
[79]Ulrich H,Katharina J,Hermann B.Towards growth of arbscular mycorrhizal fungi independent of a plant host[J].Applied and Environmental Microbiology,2002,68(4):1919-1924.
[80]彭生斌,沈崇尧.丛枝-泡囊菌根真菌-中国球囊霉在离体条件下的生长和产孢[J].科学通报,1989,34(19):1508-1511.
[81]彭生斌,沈崇尧.大豆愈伤组织和细胞抽提液对VA菌根真菌孢子发芽和芽管伸长的影响[J].科学通报,1990,35(3):227-229.
[82]Ishii T,Aikawa J,Nakamuta N,Sano K,Matsumoto,Kadoya K.Effect of citrus juice pomace extracts on in vitro hyphal growth of vesicular-arbuscular mycorrhizal fungi and their in vivo infections of citrus roots[J].Journal of the Japanese Society for Horticultural Science,2000,69(1):9-14.
[83]Kuwada K,Ishii T,Matsmodo I,Kadoya K.Effect of seaweed extracts on hyphal growth of vesicular—arbuscular mycorrhizal fungi and their infectivity on trifoliate orange roots [J].Journal of the Japanese Society for Horticultural Science,1999,68:321-326.
[84]Budi S W,Dvan T,Martinotti G,Gianinazzi S.Isolation from the Sorghum bicolor mycorrhizosphere of a bacterium compatible with arbuscular mycorrhiza development and antagonistic towards soil borne fungal pathogens[J].Applied and Environmental Microbiology,1999,65:5148-5150.
[85]杨晓红,李道高,石井孝昭,门屋一臣.百喜草(Paspalum notatum Flngge)茎叶发酵前后甲醇溶提物对离体条件下AMF的生长效应[J].应用与环境生物学报,2001,7(1):79-83.
[86]于富强,纪大干,刘培贵.云南松外生菌根真菌分离培养研究[J].植物研究,2003,23(1):67-72.
[87]秦岭,陈原,王有智.15种外生菌根真菌在4种培养基上的生长效果研究[J].北京农学院学报,1997,12(1):30-33.
[88]Joanna F D,Straker C J,Mary C S.Ecological and anatomical character of some Pinus patula ectomycorrhizas from Mpumalanga.South Africa[J].Mycorrhiza,1999,9:9-24.
[89]Jeffrey K S,Polishook J D,White J F.Endo—phytic Fungi[A].In:Gregory M.Mueller,Gerald F.Bills,Mercedes S.Foster.Biodiversity of Fungi,Inventory and Monitoring Methode[M].New York:Elsevier Academic Press,2004:241-270.
[90]李明,张灼,彭彦华.兰科菌根研究与应用[J].云南农业科学,2000(6):42-44.
[91]Currah R S,Zelmer C,Hambleton S.New records and new taxa of fungi from the mycorrhizae of terrestrial orchids of Alberta[J].Can J Bot,1987,65:2473-2482.
[92]Moore R T.The genera of Rhizoctonia-like fungi:Ascorhitonia,Ceratorhiza gen.nov.,Epulorhiza gen nov.,Monitiopsis,and Rhizoctonia[J].Mycotaxon.1987,29:91-99.
[93]Andersen T F.A comparative taxonomic study of Rhizoctonia sensu lato employing morphological,ultrastructural and molecular methods[J].Mycological Research,1996, 100(9):1117-1128.
[94]王发园,刘润进.AM真菌分类学最新研究进展[J].菌物系统,2001,20(4):588-593.
[95]Morton J B.Redescription of Glomus caledonium based on correspondence of spore morphological character in type specimens and a living reference culture[J].Mycorrhiza,1996,6:161-166.
[96]St(u|¨)rmer S L,Morton J B.Developmental patterns defining morphological characters in spores of species in Glomus(Glomales,Zygomycetes)[J].Mycologia,1997,89:72-81.
[97]Walker C.Taxonomic concepts in the Endogonaceae:spore wall concepts in species descriptions[J].Mycotaxon,1983,18:443-455.
[98]Morton J B.Effects of mountants and fixatives on morphology and Melzer's reaction of spores of two Acaulospora species(Endogonaceae)[J].Mycologia,1986,78:787-794.
[99]Morton J B,Benny G L.Revised clas sification of arbuscular mycorrhizal fungi (Zygomycetes):a new order,Glomales,two new suborders.Glominae and Gigasporinae,and two families,Acaulosporaceae and Gigasporaceae,with an emendation of Glomaceae[J].Mycologia,1990,80:20-524.
[100]Morton J B.Three new species of Acaulospora(Endogonaceae) from high aluminum,low pH soils in West Virginia[J].Mycologia.1986,78(4):641-648.
[101]Franken P,Lapopin L,Meyegaue G.RNA accumulation and genes expressed in spores of the arbuscular mycorrhizal a fungus,Gigasporarosea[J].Mycologia,1997,89(2):293-297.
[102]Sch(u|¨)βler A,Schwarzott D,Walker C.A new fungal phylum,the Glomeromycota:Phylogeny and evolution[J].Mycol.Res.2001,105:1413-1421.
[103]Redecker D.Specific PCR primers to identify arbuscular mycorrhizal fungi within colonized roots[J].Mycorrhiza.2000,10:73-80.
[104]Morton J B,Redecker D.Two new families of Glomales,Archaeosporaceae and Paraglomaceae,with two new genera Archaeospora andParagiom,based on concordant molecular and morphological characters[J].Mycologia.2001,93(1):181-195.
[105]Carsten Renker,Jochen H,Michael K.Combining nested-PCR and restriction digest of the internal transcribed spacer region to characterize arbuscular mycorrhizas fungi on roots from the field[J].Mycorrhiza,Springer-Verlag Heidelberg,2003,191-198.
[106]Hildebrandt U,Janetta K,Ouziad F.Arbuscular mycorrhizal colonization of haophytes in Central European salt marshes[J].Mycorrhiza,2001,10:175-183.
[107]Redecker D,Morton J B,Bruns TD.Molecular phylogeny of the arbuscular mycorrhizal fungi Glomus sinuosum and Sclerocystis coremioide[J].Mycologia,2000,92(2):282-285.
[108]Sawaki H,Kojima T,Shinozaki N,Saito M.Diversity of deeply branched arbuscular mycorrhizal fungi,A rchaeospora spp.,and their detection with PCR[A].In:Abstracts of 3rd International Conference on Mycorrhizas[C].2001.
[109]Turnau K,Ryszka P,Gianinazzi-Pearson V.Identification of arbuscular mycorrhizal fungi in soils and roots of plants colonizing zinc wastes in southern Poland[J].Mycorrhiza.2001,10(4):169-174.
[110]王发园,朱正杰,张显伦,刘润进[J].分子标记技术在丛枝菌根研究中的应用现状.莱阳农学院学报,2002,19(2):119-123.
[111]Simon L,Levesque R C,Lalonde M.Identification of endomycorrhizal fungi colonizing roots using SSCP[A].In:Abstracts of Oral and Poster Presentation of the 9th NACOM [C].1993.
[112]Kjoller R,Rosendahl S.Detection of arbuscular mycorrhizal fungi(Glomales) in roots by nested PCR and SSCP(Single Stranded Conformation Polymorphism)[J].Plant Soil,2000,226:189-196.
[113]龙良鲲,姚青,羊宋贞,朱红惠.一株丛枝菌根真菌的形态与分子鉴定[J].华南农业大学学报,2006,27(4):40-42.
[114]Zhao Z,M a K X,Duan A A.Research on types and ecological characteristics of ectomycorrhiza of Populus tan entosa[A].In:Hua X M.Studies on Mycorrhiza of forest trees[M].Beijing:Chinese Science and Technology Press,1995:120-125.
[115]Huang Y C,Huang Y Q,Wang Y Z.Ectomycorrhizae formed by fungi in the genus Lactarius on Pinus[J].Acta Mycol,1996,15(4):278-283.
[116]Mehmann B.N ucleo tide sequence variation of ch itin syntheses genes among ectomyco rrh izal fungi and its po tential use in taxonomy[J].Appl.Environ.Microbiol.,1994,60(9):3105-3111.
[117]Erland S,Martin F,Martin F.Identification of the ectomycorrhizal basidionycete Tylospora fibrillose Donk by RFLP analysis of the PCR-amplified ITS and IGS regions of ribosomal DNA[J].New Phytologist,1994,126:525-532.
[118]陈应龙,弓明钦,徐大平,仲崇录.等菌根生物多样性及其应用研究[M].北京:中国林业出版社,2000,107-112.
[119]王宇,郭良栋.内生真菌Ⅱ.中国油松内生真菌四个新纪录种[J].菌物学报,2004,23(1):24-27.
[120]范黎,郭顺星,曹文芩,肖培根,徐锦堂.墨兰共生真菌一新种的分离.培养.鉴定及其生物活性[J].真菌学报,1996,15(4):251-255.
[121]伍建榕,韩素芬,朱有勇,吕梅,王光平,郭文林.云南兰科植物菌根内生真菌种类研究[J].西南林学院学报,2006,26(3):5-10.
[122]李长田,李玉,方浙明,成昌根.东北红豆杉内生真菌的多样性[J].吉林农业大学学报,2004,6:612-615.
[123]Harley J L,Smith S E.Mycorrhizal Symbiosis[M].London:Academic Press,1983:268-295.
[124]Currah R S,Zelmer C,Hambleton S.New records and new taxa of fungi from the mycorrhizae of terrestrial orchids of Alberta[J].Can J Bot,1987,65:2473-2482.
[125]Currah R S,Zelmer C.A key and notes for the genera of fungi mycorrhizal with orchids and a new species in the genus Epulorhiza[J].Rept Tottori Mycol Inst,1992,30:43-59.
[126]郭顺星,曲文苏,高微微.铁皮石斛及金钗石斛菌根真菌的分离及其生物活性测定[J].中国中药杂志,2000,25(6):338-341.
[127]李明,张灼,缪锦梅.对一种建兰栽培品种菌根真菌的调查[J].大理师专学报,2000,1:50-51.
[128]Frank A B.Uber die auf Wurzeisymbiose beruhende Ernahrung gewisser Baume durche unterirdische Pilze[J].Brr Deutsch Bot Gessell,1885,3:128-145.
[129]王发园,林先贵,周健民.中国AM真菌的生物多样性[J].生态学杂志,2004,23(6):149-154.
[130]彭生斌,沈崇尧,裘维蕃.中国的内囊霉科菌根真菌[J].真菌学报,1990,9(3):169-175.
[131]邢晓科,李玉,Yolande Daple.吉林省参地中的10种VA菌根真菌[J].吉林农业大学学报,2000,22(2):41-46.
[132]张美庆,王幼珊,王克宁,邢礼军.我国东南沿海的VA菌根真菌-Ⅱ.球囊霉属四个种[J].真菌学报,1996b,15(4):241-246.
[133]张美庆,王幼珊,邢礼军.球囊霉目一新种:长孢球囊霉[J].菌物系统,1997,16(4):241-243.
[134]张美庆,王幼珊,王克宁,邢礼军.我国东南沿海地区的VA菌根真菌Ⅲ.无梗囊霉属7个我国新记录种[J].菌物系统,1998,17(1):15-18.
[135]张美庆,王幼珊.我国北部的七种VA菌根真菌[J].真菌学报,1991,10(1):13-21.
[136]张美庆,王幼珊,邢礼军.广西平果铝矿区的三个AM真菌新记录种[J].菌物系统,2001,20(2):271-272.
[137]张英,郭良栋,刘润进.都江堰亚热带地区常见植物根围的丛枝菌根真菌[J].菌物系统,2003,22(2):204-210.
[138]张英,郭良栋.中国丛枝菌根真菌两新纪录种[J].菌物学报,2005,24(3):465-467.
[139]胡弘道.杉木与台湾杉内生菌根之研究[J].中华林学季刊,1988,21(2):45-72.
[140]胡弘道.Glomus spi nosum sp.nov.in the Glomaceae from Taiwan[J].Mycotaxon,2002.83:159-164.
[141]盖京苹,刘润进.野生植物根围的丛枝菌根真菌Ⅰ[J].菌物系统,2000,19(1):24-28.
[142]石兆勇,陈应龙,刘润进.西双版纳地区龙脑香科植物根围的AM真菌[J].菌物系统,2003,22(3):402-409.
[143]Wu CG,Liu YS,Hwuang L.omales of Taiwan:V.Glomus chimonobambusae and Ent rophospora kentinesis spp.nov[J].Mycotaxon,1995,5:283-294.
[144]刘润进,王发园,孟祥霞.渤海湾岛屿的丛枝菌根真菌[J].菌物系统,2002,21(4):525-532.
[145]刘延荣,方宇澄,黄镇.山东烟区土壤VA菌根真菌的分离鉴定[J].吉林农业大学学报,2001,23(1)40-45.
[146]李涛,李建平,赵之伟.丛枝菌根真菌的两个中国新记录种[J].菌物学报,2004,23(1):144-145.
[147]唐振尧,臧穆.内囊霉科检索表的增补和新种—柑橘球囊霉[J].云南植物研究,1984,6(3):295-304.
[148]方宇澄,刘延荣,方榕.烟草内生菌根真菌的分离鉴定[J].真菌学报,1986,5(3):185-190.
[149]吴继光,陈瑞青.台湾内生菌之调查Ⅰ.溪头地区孟宗竹林内生菌科之调查[J].Taiwania,1986,31:65-88.
[150]吴继光,陈瑞青.台湾的内囊霉科Ⅱ.Sclerocystis的两个新种[J].中菌会刊,1987,2(2):73-83.
[151]吴铁航,郝文英,林先贵,施亚勤.我国VA菌根真菌的两个新记录种[J].真菌学报,1994,13(4):331-332.
[152]吴铁航,郝文英,林先贵,施亚勤.红壤中VA菌根真菌(球囊霉目)的种类和生态分布[J].真菌学报,1995,14(2):81-85.
[153]吴重华,王吉忍,杨俊秀,刘立华.太白山自然保护区AM真菌资源调查研究[J].西北林学院学报,2001,16(2):35-39.
[154]吴重华,唐明,马义生,李学林.太白山自然保护区巴山冷杉(Abies fargesii Franch)根际土壤中的5种AM真菌[J].西北林学院学报,2000,15(2):49-52.
[155]王平,胡正嘉.棉花VA菌根真菌的分离鉴定[J].华中农业大学学报,1989,8(1):36-44.
[156]王发园,刘润进.丛枝菌根真菌一新种:枣庄球囊霉[J].菌物系统,2002,21(4):522-524.
[157]王幼珊,张美庆,王克宁,邢礼军.我国东南沿海地区的AM真菌Ⅳ.四个我国新记录种[J].菌物系统,1998,17(4):301-303.
[158]潘幸来,张贵云,王永杰,吴慎杰.黄土高原的VA菌根真菌Ⅳ[J].菌物系统,1997,16(3):166-168.
[159]潘幸来,张贵云,王永杰.黄土高原的VA菌根真菌(Ⅲ)—新纪录种橙棕色盾巨孢囊霉及其与异配盾巨孢囊霉的比较[J].山西大学学报,1996,19(2)187-190.
[160]潘幸来,张贵云,王永杰,吴慎杰.黄土高原的一个VA菌根真菌新种:三红盾巨孢囊霉[J].菌物系统,1997,16(3):169-171.
[161]赵之伟,杜刚.云南热带蕨类植物根际土壤中的六种VA菌根真菌[J].菌物系统,1997,16(3):208-211.
[162]赵之伟.云南热带,亚热带蕨类植物根际土壤中的VA菌根真菌[J].云南植物研究,1998,20(2):183-192.
[163]汪洪刚,吴观以,李慧全.一个我国内囊霉科实果内囊霉属新记录的种2弯丝波纹状实果内囊霉[J].真菌学报,1992,11(1):78-79.
[164]杨安娜,李凌飞,赵之伟.中国丛枝菌根真菌一新记录种[J].菌物学报,2004,23(4):603-604.
[165]乔红权,张英,郭良栋,傅俊范.新疆北部地区常见植物根围的丛枝菌根真菌[J].菌物学报,2005,24(1):130-136.
[166]Trappe J M.Fungus associates of ectatrophic mycorrhizae[J].Bot Rev,1962,28:538-606.
[167]Smith S E,Read D J.Mycorrhizal Symbiosis(2 nd Ed)[M].California:ACADEMIC PRESS,1997.
[168]郭秀珍,毕国昌.林木菌根及应用技术[M].北京:中国林业出版社,1989.
[169]谭方河,王云璋.四川松树,桉树外生菌根菌种类调查[J].四川林业科技,2000,21(3):65-69.
[170]陈辉,唐明.杨树菌根研究进展[J].林业科学,1997,33(2):181-188.
[171]Harley J L,Sm ith S E.Mycorrhizal Symbiosis[M].Academic Press,London,1983,1-483.
[172]Fisher P J,Pctrini O.Ecology,biodiversity and Physiology of endophytic fungi[J].Curr.Top.Bot.Res,1993,1:271-279.
[173]Hyde K D,D L Hawksworth.Measuring and monitoring the biodiversity of microfungi [A].In:K D Hyde.Biodiversity of Tropical Microfungi[M].Hong Kong:Hong Kong University Press,1997:11-28.
[174]McNeely J A.Strategies of conserving biodiversity[J].Environment.1990,32(3):16-42.
[175]施立明.遗传多样性及其保存[J].生物科学信息,1990,(2):159-164.
[176]沈浩,刘登义.遗传多样性概述[J].生物学杂志,2001(6):6-9.
[177]李运贤,李玉英,邢倩.植物多样性的分子生物学研究方法[J].南阳师范学院学报,2005,4(9):52-56.
[178]邱芳,伏建民,金德敏,王斌.遗传多样性的分子检测[J].生物多样性,1998,6(2):143-150.
[179]潘莹,赵桂仿.分子水平的遗传多样性及其测量方法[J].西北植物学报,1998,18(4):645-653.
[180]Mukai Y,Suyama Y,Tsuotura Y,Kawahara T.A liokage map for sugi(Gyptomeria japonica) based on RFLP,RAPD and isozyme foei[J].Theor Appl Genet,1995,90:835-840.
[181]Williams J G K,Kubelik A R,Livak J.DNA polymorphisms amplified by arbitary primers are useful as genetic markers[J].Nucleic Acid Research,1990,18:6531-6535.
[182]Welsh J,MmClelland M.Fingerprinting genomes using PCR with arbitary primers[J].Nucleic Acids Research,1990,18:7213-7218.
[183]任旭琴.遗传多样性及其研究方法[J].淮阴工学学报,2002,11(5):6-7.
[184]谢佳燕,张知彬.ISSR技术及其在遗传多样性研究中的应用[J].兽类学报,2004,24(1):71-76.
[185]Zabeau M,Vos P.Selective restriction fragment amplification:a general method for DNA fin gerprinting[P].European Patent Application No:0534858,1993:
[186]李璇,王晓东.AFLP的研究[J].甘萧教育学院学报,2004,18(1):58-61.
[187]李伟,纪燕玲,于汉寿,莫凌霄,李飞凤.王志伟中国产禾本科植物内生真菌的遗传多样性及rDNA-ITS系统发育分析[J].菌物学报,2006,25(2):217-226.
[188]Christoph R,Gr(u|¨)nig Thomas N.Sieber.Molecular and phenotypic description of the widespread root symbiont Acephala applanata gen.et sp.nov.,formerly known as dark-septate endophyte Type 1[J].Mycologia,2005,97(3):628-640.
[189]Ajay Kumar,Pandey,Mang Jye Ger,Hsiang En Huang,Mei Kuen Yip,Jiqing Zeng,Teng-Yung Feng.Sweet pepper ferredoxin-like protein(pflp)gene as a novel selection marker for orchid transformation[J].Planta,2003.
[190]魏宇昆,高玉葆,李川,许华,任安芝.内蒙古中东部草原羽茅内生真菌的遗传多样性[J].植物生态学报,2006,30(4):640-649.
[191]Lappalainen J H,Yli Mattila T.Genetic diversity in Finland of the birch endophyte Genomonia setacea as determined by RAPD-PCR markers[J].Mycol Res,1999,103:328-332.
[192]Shahasi Yusuf Athman.Host-endophyte-pest interactions of endophytic Fusarium oxysporum antagonistic to Radopholus similis in banana(Musa spp)[D].Athman S Y:University of Pretoria,2006:
[193]Prosser J I.Molecular and functional diversity in soil micro-organisms[J].Plant and Soil,2002,244:9-17.
[194]Chelius M K,Triplett E W.Rapid detection of arbuscular mycorrhizae in roots and soil of an intensively managed turfgrass system by PCR amplification of small subunit rDNA [J].Mycorrhiza,1999,9:61-64.
[195]Tonin C,Vandenkoornhuyse P,Joner EJ.Assesssment of arbuscular mycorrhizal fungi diversity in the rhizosphere of Viola calami naria and effect of these fungi on heavy metal uptake by clove[J].Mycorrhiza,2001,10(4):161-168.
[196]Tonin C,Vandenkoornhuyse P,Joner E J.Effect of metallic trace on AM fungi diversity within roots of Trifolium subterraneum colonised with AM spores isolated from a polluted soil:application to soil remediation[A].In:Abstracts of 3rd International Conference on Mycorrhizas[C].2001.
[197]Ridgway K P,Marland L A,Young J P W.Molecualr diversity of mycorrhizal fungi on the roots of trees grown with contaminated soil inoculum[A].In:Abstracts of 3rd International Conference on Mycorrhizas[C].2001.
[198]Renker C,Kaldorf M,Buscot F.Structural diversity of arbuscular mycorrhizal fungi in a disturbed grassland[A].In:Abstracts of 3rd International Conference on Mycorrhizas[C].2001.
[199] Redecker D. Specific PCR primers to identify arbuscular mycorrhizal fungi within colonized roots [J] . Mycorrhiza, 2000,10: 73-80.
[200] Hijri M, Redecker D, Sanders I R. Scutellospora castanea spores as microbial communities [A] . In: Abstracts of 3rd International Conference on Mycorrhizas[C],2001.
[201] Versaw. W. K, Chiou. T, Harrison. M. J. Phosphate transporters of Medicago truncatula and arbuscular mycorrhizal fungi [J] . Plant and Soil, 2002,244: 239-245.
[202] Requena N, Mann P, Hampp R, Franken P. Early developmentally regulated genes in the arbuscular mycorrhizal fungus Glomus mosseae: identification of GmGINI, a novel gene with homology to the C-terminus of metazoan hedgehog proteins [J] . Plant and Soil,2002,224: 129-139.
[1]Guo L D,Hyde K D,Liew E C.A method to promote sporulation in palm endophytic fungi[J].Fungal Diversity,1998,1:109-113.
[2]王静,任交芝,谢凤行,魏宇昆,高玉葆.几种诱导黑麦草Lolium perenne L.内生真菌产孢的方法[J].菌物学报,2005,24(4):590-596.
[3]郭春秋,罗永兰,张志远.几种真菌的诱导产孢试验[J].海南大学学报自然科学版,2003,21(1):74-77.
[4]Bills G F,Polishook J D.Recovery of endophytic fungi from Chamaecyparis thyroids [J].Sydowia,1992,44:1-12.
[5]Gene J,Mercado-Sierra A,Guarro J.Dactylaria cazorlii and Hansfordia catalonica,two new hyphomycetes from litter in Spain[J].Mycol.Res.2000,104:1404-407.
[6]Kirk P M.New or interesting microfungi XV.Miscellaneous hyphomycetes from the British Isles[J].Trans.Br.Mycol.Soc.1986,86:409-428.
[1]郭顺星,徐锦堂.真菌在罗河石解和铁皮石解种子萌发中的作用[J].中国医学科学院学报,1991,13(1):46-47.
[2]吴智彪、宋希强、李绍鹏.华石斛内生真菌诱导子对其组培苗生长的影响[J].热带作物学报,2006,27(1):77-79.
[3]杨友联、刘作易、朱国胜、毛堂芬.独蒜兰菌根真菌对环草石斛生长的影响[J].种子,2008,27(1),34-37.
[4]魏景超.真菌鉴定手册[M].上海:科学出版社,1979.
[5]H L巴尼特,B.B.亨特.半知菌属图解[M].北京:科学出版社,1977.
[6]Ellis M B.Dematiaceous hyphomycetes.Ⅷ[J].Mycol.Pap.1967,111:29.
[7]Helgard I N,Kerry O'Donnell.New Fusarium species and combinations within the Gibberella fujikuroi species complex[J].Mycologia,1985,90(3):434-458.
[8]Emory G S.Alternaria themes and variations[J].Mycotaxon,1997,65(8):1-91.
[9]Emory G S.Alternaria themes and variations[J].Mycotaxon,1986,25(1):287-308.
[10]Gams W.Cephalosporium-like hyphomycetes:some tropical species[J].Trans.Br.Mycol.Soc.1975,64(3):389-404.
[11]Hermanides E Jf.Aureobasidium and allied genera[J].Stud.Mycol.1977,15:141-166.
[12]C.布斯.镰刀菌属[M].北京:北京大学出版社,1987.
[13]Stanley J.Hughes.Sooty moulds.Mycologia[J].1976,68(4):693-820.
[14]White J F,Morgan J.Endophyte-host association in forage grasses Ⅷ.A cremonium chilense,an undescribed endophyte occurring in dactylic glomerata in chile[J].Mycotaxon,1990,39:441-452.
[15]Helgard I Nirenberg,Kerry O'Donnell.New Fusarium species and combinations within the Gibberella fujikuroi species complex[J].Mycologia,1985,90(3):434-458.
[16]Castaneda R F,Kendrick B,Guarro J.Notes on conidial fungi.Ⅺ Ⅴ.New hyphomycetes from cuba[J].Mycotaxon,1997,65:93-105.
[17]Dowsett J A,Reid J,Hopkin A.On cephalosproium balanoides drechsler[J].Mycologia,1982,74(4):687-690.
[18]Niger L,Hywel J,Harry C E,Yan J.Are-evaluation of the leafhopper pathogen Torrubiella hemipterigena,its anamorph Verticillium hemipterigenum and V.pseudohemipterigenum sp.Nov[J].Mycological Research.,1997,101(10):1242-1246.
[19]Barron G L.New species and new records of fungi that attack microscopic animals[J].Can.J.Bot.,1989,67(1):267-271.
[20]Walter M.Jaklitsch,Gary J S,Sarah L D,Bing-Sheng Lu,Irina S D.Hypocrea rufa/Trichoderma viride:a reassessment,and description of five closely related species with and without warted conidia[J].Stud Mycol.,2006,56(1):135-177.
[21]David M.The genus thielavia[J].Mycologia,1973,65(5):1055-1077.
[22]Smalley E.B.,Hansen H N.The perfect stage of Gliocaldium roseum[J].Mycologia,1957,49:432-499.
[23]David J C.A contribution to the systematics of Cladosporium[J].Mycological papers,1997,172:25-31.
[24]叶琪明.中国南方镰孢属的研究Ⅱ:马特组Section Martiella的分类研究[J].浙江农业学报,2000,12(6):378-381.
[25]Hughes S J.Revisiones hyphomycetum aliquot cum appendice de nominibus rejiciendis[J].Canadian Journal of Botany.1958,36:727-836.
[26]Sutton B C.Microfungi on Xanthorrhoea[J].Transactions of the British Mycological Society 1980,(85):239-255.
[27]郭良栋.内生真菌研究进展[J].菌物系统,2001,20(1):148-152.
[28]Petrini O,Carrol G C.Endophytic fungi in folige of some Cupressaceae in Oregon[J].Canadian Journal of Botany.1981,59:629-636.
[29]Petrini O.Taxonomy of endophytic fungi of aerial plant tissues.In microbiology of the phyllosphere[M].Canbrige,Cambrige University Press,1986.
[30]范黎,郭顺星,徐锦堂.我国部分兰科植物菌根的内生真菌种类研究[J].山西大学学报,1998,21(2):169-177.
[31]张廷威,康冀川.石斛内生真菌的研究概述[J].山地农业生物学报,2005,24(5):438-441.
[32]张廷威.石斛茎部内生真菌的分离与分布[J].贵州农业科学,2007,35(2):18-19.
[1]Guo L D,Hyde K D & Liew E C Y.A method to promote sporulation in palm endophytic fungi[J].Fungal Diversity,1998,1:105-109.
[2]Guo L D,Hyde K D & Liew E C Y.Identification of endophytic fungi from Livistona chinensis(Palmae) using morphological and molecular techniques[J].New Phytologist,2000,147:617-630.
[3]Pandey A K,Reddy M S,Suryanarayanan T S.ITS-RFLP and ITS sequence analysis of a foliar endophytic Phyllosticta from different tropical trees[J].Mycol Res,2003,107:439-444.
[4]Thompson J D,Gibson T J,Plewnicak F,Higgins.D G.The ClustalX windows interface:flexible strategies for myltiple sequence alignment aided by quality analysis tools[J].Nucleic Acids Research,1997,24:4876-4882.
[5]范黎,郭顺星,徐锦堂.我国部分兰科植物菌根的内生真菌种类研究[J].山西大学学报,1998,21(2):169-177.
[6]张廷威、康冀川.石斛内生真菌的研究概述[J].山地农业生物学报,2005,24(5):438-441.
[7]Murali T S,Suryanarayanan TS,Venkatesan G.Fungal endophyte communities in two tropical forests of southern India:diversity and host affiliation[J].Mycol.Progress,2007,6:191-199.
[8]Thomas J,Bastian B,Gerhard G,Daniel B,Claire D,Marc-Andre S.Mixotrophy in orchids:insights from a comparative study of green individuals and onphotosynthetic individuals of Cephalanthera damasonium[J].New Phytologist,2005,166:639-653.
[2]Carroll G C.The biology of endophytism in plants with particular reference to woody plants[A].In:Fokkema N J,van den Heuvel J.Microbiology of the Phyllosphere[M].Cambridge,U.K:Cambridge University Press,1986:205-222.
[3]Petrini O.Fungal endophytes of tree leaves[J].New York Springerverlag,1991,179-197.
[4]郭良栋.内生真菌研究进展[J].菌物系统,2001,20(1):148-152.
[5]官珊,钟国华,孙之潭,胡美英.植物内生真菌的研究进展[J].仲恺农业技术学院学报,2005,18(1):61-66.
[6]Petrini.Taxonomy of endophytic fungi of aerial plant tissues[A].In N.J.Fokkema and J.van denHeuvel.Microbiology of the Phyllosphere[M].England:Cambridge University Press,1986:175-187.
[7]Guo L D,Hyde K D,Liew E C Y.Identification of endophytic fungi from Livistona chinensis based on morphology and Rdna sequences[J].New phytol,2000,147:617-630.
[8]Sinclair J B,Cerkauskas.Latent infection vs.endophytic colonization by fungi[A].In:Redlin S C,Carris L Meds.Endophytic Fungi in Grasses and Woody Plants:Systematics,Ecology,and Evolution[M].St.Paul,Minnesota:APS Press,1996:3-29.
[9]Zheng R,Jiang H.Rhizomucor endophyticus sp.nov.,an endophytic zygomycetes from higher plants[J].Mycotaxon,1995,56:455-466.
[10]Siegel M R,Johnson M C,Varney D R.A fungal endophytic in tall fescue:ncidence and dissemination[J].Phytopathology,1984,74:932-937.
[11]Ananda K,Sridhar K R.Diversity of endophytic funni in the toots of mannrove species on the west of India[J].Can J Microbiol,2002,48(10):871-878.
[12]徐锦堂,郭顺星.供给天麻种子萌发营养的真菌-紫萁小菇[J].真菌学报,1989,8(3):221-226.
[13]郭顺星,徐锦堂.密环菌隔膜发育的超微结构研究[J].中国医学科学院学报,1991, 13(1):46-49.
[14]郭顺星,范黎,曹文芩,陈晓梅.菌根真菌一新种-石斛小姑[J].菌物系统,1999,18(2):141-144.
[15]郭顺星,陈晓梅,于雪梅,范黎.金线莲菌根真菌的分离及其生物活性研究[J].中国药学杂志,2000,35(7):443-445.
[16]Strobel G.A proposed new taxon for a bulbilliferous hyphomycetes associated with pacific yew(Taxus brevifolia)[J].Mycotaxon,1993,XLV Ⅱ:(April-June):71-80.
[17]郭波,李海燕,张玲琪.一种产长春新碱内生真菌的分离[J].云南大学学报(自然科学版),1998,20(3):214-215.
[18]KUMAR D,SIVA Sundara,CHEUNG Honyeung.In vitro studies of endophytic funni from Tripterygium wilfordii with anti-proliferative acrivity on human peripheral blood mononnuclear cells[J].Journal of Ethnopharmacology,2004,94(2-3):295-300.
[19]Petrini O,Fisher P J.A comparative study of fungal endophytes in xylem and whole stern of pinus sylvestris and Fagus sylvatica[J].Trans Br Mycol Soc,1988,91:233-238.
[20]Toti L,Viret O,Chapela I H,Petrini O.Differential attachment by the conidia of the endophyte Discula umbrinella(Berk and Br.) Morelet to host and non-host surface[J].New Phytol,1992,121:469-475.
[21]Petrini O.Ecological and physiological aspects of host specificity in endophytic[A].In:Redlin S C,Carris L Meds.Endophytic Fungi in Grasses and Woody Plants:Systermatics,Ecology,and Evolution[M].St Paul,Minnesora:APS Press,1996,87-100.
[22]Fisher P J,Petrini O,Sutton B C.A comparative study of fungal endophytes in leaves,xylem and bark of Eucalyptus nitens in Australia and England[J].Sydowia,1993,45:338-345.
[23]范黎,郭顺星.兰科植物菌根真菌的研究进展[J].微生物学通报,1998,25(4):227-231.
[24]Peterson R L,Currah R S.Synthesis of mycorrhizae between protocorms of Goodyera repens(Orchidaceae) and Ceratobasi di um cereale[J].Can J Bot,1990,68:1117-1125.
[25]Rasmussen H N.Terrestrial orchids from seed to mycotrophic plant[M].New York:the press syndicate of the University of Cambridge,1995,1-237.
[26]范黎,郭顺星,肖培根.十九种兰科植物的内生担子菌[J].热带作物学报,1998,19(4):77-82.
[27]陈瑞蕊,林先贵,施亚琴.兰科菌根的研究进展[J].应用与环境生物学报,2003,9(1):97-101.
[28]郭顺星,曲文苏,高微微.铁皮石斛及金钗石斛菌根真菌的分离及其生物活性测定[J].中国中药杂志,2000,25(6):338-341.
[29]王利娟,贺新生.植物内生真菌分离培养的研究方法[J].微生物学杂志,2006,26(4):55-60.
[30]Masahide Yamato,Takahiro Yagame,Akira Suzuki,Koji Iwase.Isolation and identification of mycorrhizal fungi associating with an achlorophyllous plant,Epipogium roseum(Orchidaceae)[J].Mycoscience,2005,46:73-77.
[31]刘润进,李晓林.丛枝菌根及其应用[J].北京:科学出版,2000:1-186.
[32]杨显志,邵华,周成,宣群,杨春热,张玲琪.丛枝菌根研究及应用[J].云南农业 科技,2001,4:38-41.
[33]Harley J L,Smith S E.Mycorrhizal Symbiosis[M].London19:Academic Press,1983:1-483.
[34]Jeffrey K S,Polishook J D,White J F.Endophytic Fungi.In Gregory M.Mueller,Gerald F.Bills,Mercedes S.Foster.Biodiversity of Fungi,Inventoryan d Monitoring Methode[M].NewYork:Elsevier Academic Press,2004:241-270.
[35]弓明饮,陈应龙,仲崇禄.菌根研究及应用[M].北京:中国林业出版社,1997.
[36]Schulz B,Wanke U.Endophytes from erbaceous plants and shrubs:effectivness of surface-sterilization methods[J].Mycological Research,1993,97:1447-1450.
[37]张纪忠.微生物分类学[M].上海:复旦大学出版社,1990,341-365.
[38]Bills G F,Polishook J D.Microfungi from Carpinus careliana[J].Canadian Journal of Botany,1991,69:1477-1482.
[39]Bills G F,Polishook J D.Recovery of endophytic fungi from Chamaccyparis thyoides[J].Sydowia,1992,44:1-12.
[40]范黎,郭顺星,徐锦堂.我国部分兰科植物菌根的内生真菌种类研究[J].山西大学学报,1998,21(2):169-177.
[41]Olinto L P,Christtianno L R,Arnaldo C B,Kiyoshi M,Maria C M K.Epulorhiza epiphytica sp.nov.isolated from mycorrhizal roots of epiphytic orchids in Brazil[J].Mycoscience,2003,44:153-155.
[42]Stewart S,Kane M.Symbiotic seed germination of Habenaria macroceratitis (Orchidaceae),a rare Florida terrestrial orchid[J].Plant Cell,Tissue and Organ Culture,2006,82:159-167.
[43]张美庆,王幼珊.VA真菌的分类鉴定[J].华北农学报,1989(4):115-120.
[44]杨晓红,孙中海,邵菊芳,仝瑞建.丛枝菌根真菌培养方法研究进展[J].菌物学报,2004,23(3):444-456.
[45]Biermann B,Linderman R G.Use of vesicular-arbuscular mycorrhizal roots,intraradical vesicles and extraradical vesicles as inoculum[J].New Phytologist,1983,95:97-105.
[46]Diop T A,Plenchette C,Strullu D G.Dual axenic culture of sheared-root inocula of vesicular—arbuscular mycorrhizal fungi associated with tomato roots[J].Mycorrhiza,1994,5:17-22.
[47]Sylvia D M,Schenck N C.Application of superphosphate to mycorrhizal plants stimulates sporulation of phosphorus-tolerant vesicular-arbuscular mycorrhizai fungi[J].New Phytol,1983,95:655-661.
[48]Gilmore A E.Phytomycetous mycorrhizal organisms collected by open-pot culture methods[J].Hilgardia,1968.39:87-105.
[49]刘润进,李晓林.丛枝菌根及其应用[M].北京:科学出版社,2000,1-186.
[50]沈廷厚,万水林.柑桔VA菌根真菌繁殖及接种技术的应用研究[J].江西农业学报,1994,6(1):25-30.
[51]Ames R N,Linderman R G.The growth of easter lily(Lilium longiflorum) as influenced by vesicular-arbuscular mycorrhizal fungi,Fusarium oxysporum,and fertility level[J].Can J Bot,1978,56:2773-2780.
[52]Sylvia D M,Jarstfer A G.Production of inoculum and inoculation with arbuscular mycorrhizal fungi[A].In:Robson A D,Abbott L K,Malajczuk N.Management of Mycorrhizas in Agriculture,Horticulture and Forestry[M].New York:Kluwer,1994:1-248.
[53]Mosse B.The establishment of vesicular-arbuscular mycorrhiza under asetic conditions [J].J Gen Microbiol,1962,27:509-520.
[54]Crush J R,Hay M J.A technique for growing mycorrhizal clover in solution culture[J].N Z Journal of Agricultura Research,1981,24:371-372.
[55]陈应龙,弓明钦,王凤珍,陈羽.VA菌根菌剂的生产及其接种潜力的测定[J].热带亚热带土壤科学,1997,6(2):113-120.
[56]Elmes R P,Mosse B.Vesicular arbuscular endomycorrhizal inoculum production.11.Experiments with maize(Zea mays) and other hosts in nutrient flow culture[J].Can J Bot,1984,62:1531-1536.
[57]Hung V G,Sylvia D M.Production of vesicular—arbuscular mycnrrhizal fungus inoculum in aeroponic culture[J].Applied and Enviromental Microbiology,1988,54(2):353-357.
[58]Carruthers S.Aeroponics system review[J].Prac Hydropon July/August,1992,18-21.
[59]Jarstfer A G,Sylvia D M.Aeroponic culture of VAM fungi[A].In:Varma A,Hock B.Mycorrhiza-Structure,Function,Molecular Biology and Biotechnology[M].Tokyo:Springer-Verlag,1995:1-747.
[60]Mosse B,Hepper C M.Vesicular-arbuscular mycorrhizal infections in root organ cultures [J].Physiol Plant Pathol,1975,5:215-223.
[61]Redecker D,Thierfellfer H,Werner D.A new cultivation system for arbuscular mycorrhizal fungi on glass beads[J].Angewante Botanik,1995,69(5):189-191.
[62]陈保东,冯固,李晓林,崔建宇.玻璃珠分室培养AMF方法的建立[J].菌物系统,19(2):2000,212-216.
[63]Mosse B,Hepper C M.Vesicular-arbuscular mycorrhizai infections in root organ cultures [J].Physiol Plant Pathol,1975,5:215-223.
[64]Wideman M A,Watru d L S.Sporulation of Gigaspora margarita on root culture tomato [J].Can J Microbiol,1984,30:642-646..
[65]Janardhanan K K,Gupata M L,Husain A.Dual axenic culture of sheared-root inocula of vesicular-arbuscular mycorrhizal fungi associated with tomato roots[J].Current Science,1990,59(10):509-513.
[66]赵志鹏,郭秀珍.纯种VA菌根菌剂的制备及其在泡桐试管苗上的应用[J].微生物学报,1991,31(1):32-35.
[67]彭生斌,沈崇尧.用三叶草离体根建立VA菌根[J].微生物学报,1990,30(5):375-379.
[68]Mugnier J,Mosse B.Vesicular-arbuscular mycorrhizal infection in transformed Ri T-DNA roots grown axenically[J].Phytopathology,1987,77(7):1045-1050.
[69]Becard G,Fortin J A.Early events of vesicular-arbuscular mycorrhizal formation on Ri T-DNA transformed roots[J].New Phytol,1988,108:211-218.
[70]Chabot S,Becard G,Piche Y.Life cycle of Glomus intraradices in root organ culture[J].Mycologia,1992,84:315-321.
[71]李晓林,冯固.丛枝菌根生态生理[M].北京:华文出版社,2001,1-358.
[72]毕银丽,汪洪钢,李晓林.丛枝菌根的双重培养方法及其菌丝际的建立[J].菌物系统,2000,19(4):517-521.
[73]Fortin J A,St-Arnaud M,Hamel C,Chavarie C,Jolicoeur M.Aseptic in vitro endomycorrhizal spore mass production[P].US Patent Number:5554530,1996-10-09:
[74]Burggraaf A J P,Beringer J E.Absence of Nuclea DNA Synthesis in Vesicular-Arbuscular Mycorrhizal Fungi during In vitro Development[J].New Phytologist,1989.111(1),25-33.
[75]Becard G,Pfeffer P E.Status of nuclear division in arbuscular mycorrhizal fungi during in vitro development[J].Protoplasma,1993,194:62-68.
[76]Siqueira J O.Cultura axenicae monoxenica dos fungos microrrizicos vesiculo-arbusculares. In:Reuniao Brasileira sobre[J].Micorrizas,1987,44-70.
[77]Barea J M.Importance of hormones and root exudates in mycorrhiza phenomena[A].In:Gianinazzi-Pearson V,Gianinazzi S.Physiological and Genetic Aspects of Mycorrhizae [M].Paris:INRA,1986:1-832.
[78]Thompson J.Soil sterilization methods to show VA-mycorrhizae add P and Zn nutrition of wheat in vertisols[J].Soil Biol.Biochem,1990,26:1133-1143.
[79]Ulrich H,Katharina J,Hermann B.Towards growth of arbscular mycorrhizal fungi independent of a plant host[J].Applied and Environmental Microbiology,2002,68(4):1919-1924.
[80]彭生斌,沈崇尧.丛枝-泡囊菌根真菌-中国球囊霉在离体条件下的生长和产孢[J].科学通报,1989,34(19):1508-1511.
[81]彭生斌,沈崇尧.大豆愈伤组织和细胞抽提液对VA菌根真菌孢子发芽和芽管伸长的影响[J].科学通报,1990,35(3):227-229.
[82]Ishii T,Aikawa J,Nakamuta N,Sano K,Matsumoto,Kadoya K.Effect of citrus juice pomace extracts on in vitro hyphal growth of vesicular-arbuscular mycorrhizal fungi and their in vivo infections of citrus roots[J].Journal of the Japanese Society for Horticultural Science,2000,69(1):9-14.
[83]Kuwada K,Ishii T,Matsmodo I,Kadoya K.Effect of seaweed extracts on hyphal growth of vesicular—arbuscular mycorrhizal fungi and their infectivity on trifoliate orange roots [J].Journal of the Japanese Society for Horticultural Science,1999,68:321-326.
[84]Budi S W,Dvan T,Martinotti G,Gianinazzi S.Isolation from the Sorghum bicolor mycorrhizosphere of a bacterium compatible with arbuscular mycorrhiza development and antagonistic towards soil borne fungal pathogens[J].Applied and Environmental Microbiology,1999,65:5148-5150.
[85]杨晓红,李道高,石井孝昭,门屋一臣.百喜草(Paspalum notatum Flngge)茎叶发酵前后甲醇溶提物对离体条件下AMF的生长效应[J].应用与环境生物学报,2001,7(1):79-83.
[86]于富强,纪大干,刘培贵.云南松外生菌根真菌分离培养研究[J].植物研究,2003,23(1):67-72.
[87]秦岭,陈原,王有智.15种外生菌根真菌在4种培养基上的生长效果研究[J].北京农学院学报,1997,12(1):30-33.
[88]Joanna F D,Straker C J,Mary C S.Ecological and anatomical character of some Pinus patula ectomycorrhizas from Mpumalanga.South Africa[J].Mycorrhiza,1999,9:9-24.
[89]Jeffrey K S,Polishook J D,White J F.Endo—phytic Fungi[A].In:Gregory M.Mueller,Gerald F.Bills,Mercedes S.Foster.Biodiversity of Fungi,Inventory and Monitoring Methode[M].New York:Elsevier Academic Press,2004:241-270.
[90]李明,张灼,彭彦华.兰科菌根研究与应用[J].云南农业科学,2000(6):42-44.
[91]Currah R S,Zelmer C,Hambleton S.New records and new taxa of fungi from the mycorrhizae of terrestrial orchids of Alberta[J].Can J Bot,1987,65:2473-2482.
[92]Moore R T.The genera of Rhizoctonia-like fungi:Ascorhitonia,Ceratorhiza gen.nov.,Epulorhiza gen nov.,Monitiopsis,and Rhizoctonia[J].Mycotaxon.1987,29:91-99.
[93]Andersen T F.A comparative taxonomic study of Rhizoctonia sensu lato employing morphological,ultrastructural and molecular methods[J].Mycological Research,1996, 100(9):1117-1128.
[94]王发园,刘润进.AM真菌分类学最新研究进展[J].菌物系统,2001,20(4):588-593.
[95]Morton J B.Redescription of Glomus caledonium based on correspondence of spore morphological character in type specimens and a living reference culture[J].Mycorrhiza,1996,6:161-166.
[96]St(u|¨)rmer S L,Morton J B.Developmental patterns defining morphological characters in spores of species in Glomus(Glomales,Zygomycetes)[J].Mycologia,1997,89:72-81.
[97]Walker C.Taxonomic concepts in the Endogonaceae:spore wall concepts in species descriptions[J].Mycotaxon,1983,18:443-455.
[98]Morton J B.Effects of mountants and fixatives on morphology and Melzer's reaction of spores of two Acaulospora species(Endogonaceae)[J].Mycologia,1986,78:787-794.
[99]Morton J B,Benny G L.Revised clas sification of arbuscular mycorrhizal fungi (Zygomycetes):a new order,Glomales,two new suborders.Glominae and Gigasporinae,and two families,Acaulosporaceae and Gigasporaceae,with an emendation of Glomaceae[J].Mycologia,1990,80:20-524.
[100]Morton J B.Three new species of Acaulospora(Endogonaceae) from high aluminum,low pH soils in West Virginia[J].Mycologia.1986,78(4):641-648.
[101]Franken P,Lapopin L,Meyegaue G.RNA accumulation and genes expressed in spores of the arbuscular mycorrhizal a fungus,Gigasporarosea[J].Mycologia,1997,89(2):293-297.
[102]Sch(u|¨)βler A,Schwarzott D,Walker C.A new fungal phylum,the Glomeromycota:Phylogeny and evolution[J].Mycol.Res.2001,105:1413-1421.
[103]Redecker D.Specific PCR primers to identify arbuscular mycorrhizal fungi within colonized roots[J].Mycorrhiza.2000,10:73-80.
[104]Morton J B,Redecker D.Two new families of Glomales,Archaeosporaceae and Paraglomaceae,with two new genera Archaeospora andParagiom,based on concordant molecular and morphological characters[J].Mycologia.2001,93(1):181-195.
[105]Carsten Renker,Jochen H,Michael K.Combining nested-PCR and restriction digest of the internal transcribed spacer region to characterize arbuscular mycorrhizas fungi on roots from the field[J].Mycorrhiza,Springer-Verlag Heidelberg,2003,191-198.
[106]Hildebrandt U,Janetta K,Ouziad F.Arbuscular mycorrhizal colonization of haophytes in Central European salt marshes[J].Mycorrhiza,2001,10:175-183.
[107]Redecker D,Morton J B,Bruns TD.Molecular phylogeny of the arbuscular mycorrhizal fungi Glomus sinuosum and Sclerocystis coremioide[J].Mycologia,2000,92(2):282-285.
[108]Sawaki H,Kojima T,Shinozaki N,Saito M.Diversity of deeply branched arbuscular mycorrhizal fungi,A rchaeospora spp.,and their detection with PCR[A].In:Abstracts of 3rd International Conference on Mycorrhizas[C].2001.
[109]Turnau K,Ryszka P,Gianinazzi-Pearson V.Identification of arbuscular mycorrhizal fungi in soils and roots of plants colonizing zinc wastes in southern Poland[J].Mycorrhiza.2001,10(4):169-174.
[110]王发园,朱正杰,张显伦,刘润进[J].分子标记技术在丛枝菌根研究中的应用现状.莱阳农学院学报,2002,19(2):119-123.
[111]Simon L,Levesque R C,Lalonde M.Identification of endomycorrhizal fungi colonizing roots using SSCP[A].In:Abstracts of Oral and Poster Presentation of the 9th NACOM [C].1993.
[112]Kjoller R,Rosendahl S.Detection of arbuscular mycorrhizal fungi(Glomales) in roots by nested PCR and SSCP(Single Stranded Conformation Polymorphism)[J].Plant Soil,2000,226:189-196.
[113]龙良鲲,姚青,羊宋贞,朱红惠.一株丛枝菌根真菌的形态与分子鉴定[J].华南农业大学学报,2006,27(4):40-42.
[114]Zhao Z,M a K X,Duan A A.Research on types and ecological characteristics of ectomycorrhiza of Populus tan entosa[A].In:Hua X M.Studies on Mycorrhiza of forest trees[M].Beijing:Chinese Science and Technology Press,1995:120-125.
[115]Huang Y C,Huang Y Q,Wang Y Z.Ectomycorrhizae formed by fungi in the genus Lactarius on Pinus[J].Acta Mycol,1996,15(4):278-283.
[116]Mehmann B.N ucleo tide sequence variation of ch itin syntheses genes among ectomyco rrh izal fungi and its po tential use in taxonomy[J].Appl.Environ.Microbiol.,1994,60(9):3105-3111.
[117]Erland S,Martin F,Martin F.Identification of the ectomycorrhizal basidionycete Tylospora fibrillose Donk by RFLP analysis of the PCR-amplified ITS and IGS regions of ribosomal DNA[J].New Phytologist,1994,126:525-532.
[118]陈应龙,弓明钦,徐大平,仲崇录.等菌根生物多样性及其应用研究[M].北京:中国林业出版社,2000,107-112.
[119]王宇,郭良栋.内生真菌Ⅱ.中国油松内生真菌四个新纪录种[J].菌物学报,2004,23(1):24-27.
[120]范黎,郭顺星,曹文芩,肖培根,徐锦堂.墨兰共生真菌一新种的分离.培养.鉴定及其生物活性[J].真菌学报,1996,15(4):251-255.
[121]伍建榕,韩素芬,朱有勇,吕梅,王光平,郭文林.云南兰科植物菌根内生真菌种类研究[J].西南林学院学报,2006,26(3):5-10.
[122]李长田,李玉,方浙明,成昌根.东北红豆杉内生真菌的多样性[J].吉林农业大学学报,2004,6:612-615.
[123]Harley J L,Smith S E.Mycorrhizal Symbiosis[M].London:Academic Press,1983:268-295.
[124]Currah R S,Zelmer C,Hambleton S.New records and new taxa of fungi from the mycorrhizae of terrestrial orchids of Alberta[J].Can J Bot,1987,65:2473-2482.
[125]Currah R S,Zelmer C.A key and notes for the genera of fungi mycorrhizal with orchids and a new species in the genus Epulorhiza[J].Rept Tottori Mycol Inst,1992,30:43-59.
[126]郭顺星,曲文苏,高微微.铁皮石斛及金钗石斛菌根真菌的分离及其生物活性测定[J].中国中药杂志,2000,25(6):338-341.
[127]李明,张灼,缪锦梅.对一种建兰栽培品种菌根真菌的调查[J].大理师专学报,2000,1:50-51.
[128]Frank A B.Uber die auf Wurzeisymbiose beruhende Ernahrung gewisser Baume durche unterirdische Pilze[J].Brr Deutsch Bot Gessell,1885,3:128-145.
[129]王发园,林先贵,周健民.中国AM真菌的生物多样性[J].生态学杂志,2004,23(6):149-154.
[130]彭生斌,沈崇尧,裘维蕃.中国的内囊霉科菌根真菌[J].真菌学报,1990,9(3):169-175.
[131]邢晓科,李玉,Yolande Daple.吉林省参地中的10种VA菌根真菌[J].吉林农业大学学报,2000,22(2):41-46.
[132]张美庆,王幼珊,王克宁,邢礼军.我国东南沿海的VA菌根真菌-Ⅱ.球囊霉属四个种[J].真菌学报,1996b,15(4):241-246.
[133]张美庆,王幼珊,邢礼军.球囊霉目一新种:长孢球囊霉[J].菌物系统,1997,16(4):241-243.
[134]张美庆,王幼珊,王克宁,邢礼军.我国东南沿海地区的VA菌根真菌Ⅲ.无梗囊霉属7个我国新记录种[J].菌物系统,1998,17(1):15-18.
[135]张美庆,王幼珊.我国北部的七种VA菌根真菌[J].真菌学报,1991,10(1):13-21.
[136]张美庆,王幼珊,邢礼军.广西平果铝矿区的三个AM真菌新记录种[J].菌物系统,2001,20(2):271-272.
[137]张英,郭良栋,刘润进.都江堰亚热带地区常见植物根围的丛枝菌根真菌[J].菌物系统,2003,22(2):204-210.
[138]张英,郭良栋.中国丛枝菌根真菌两新纪录种[J].菌物学报,2005,24(3):465-467.
[139]胡弘道.杉木与台湾杉内生菌根之研究[J].中华林学季刊,1988,21(2):45-72.
[140]胡弘道.Glomus spi nosum sp.nov.in the Glomaceae from Taiwan[J].Mycotaxon,2002.83:159-164.
[141]盖京苹,刘润进.野生植物根围的丛枝菌根真菌Ⅰ[J].菌物系统,2000,19(1):24-28.
[142]石兆勇,陈应龙,刘润进.西双版纳地区龙脑香科植物根围的AM真菌[J].菌物系统,2003,22(3):402-409.
[143]Wu CG,Liu YS,Hwuang L.omales of Taiwan:V.Glomus chimonobambusae and Ent rophospora kentinesis spp.nov[J].Mycotaxon,1995,5:283-294.
[144]刘润进,王发园,孟祥霞.渤海湾岛屿的丛枝菌根真菌[J].菌物系统,2002,21(4):525-532.
[145]刘延荣,方宇澄,黄镇.山东烟区土壤VA菌根真菌的分离鉴定[J].吉林农业大学学报,2001,23(1)40-45.
[146]李涛,李建平,赵之伟.丛枝菌根真菌的两个中国新记录种[J].菌物学报,2004,23(1):144-145.
[147]唐振尧,臧穆.内囊霉科检索表的增补和新种—柑橘球囊霉[J].云南植物研究,1984,6(3):295-304.
[148]方宇澄,刘延荣,方榕.烟草内生菌根真菌的分离鉴定[J].真菌学报,1986,5(3):185-190.
[149]吴继光,陈瑞青.台湾内生菌之调查Ⅰ.溪头地区孟宗竹林内生菌科之调查[J].Taiwania,1986,31:65-88.
[150]吴继光,陈瑞青.台湾的内囊霉科Ⅱ.Sclerocystis的两个新种[J].中菌会刊,1987,2(2):73-83.
[151]吴铁航,郝文英,林先贵,施亚勤.我国VA菌根真菌的两个新记录种[J].真菌学报,1994,13(4):331-332.
[152]吴铁航,郝文英,林先贵,施亚勤.红壤中VA菌根真菌(球囊霉目)的种类和生态分布[J].真菌学报,1995,14(2):81-85.
[153]吴重华,王吉忍,杨俊秀,刘立华.太白山自然保护区AM真菌资源调查研究[J].西北林学院学报,2001,16(2):35-39.
[154]吴重华,唐明,马义生,李学林.太白山自然保护区巴山冷杉(Abies fargesii Franch)根际土壤中的5种AM真菌[J].西北林学院学报,2000,15(2):49-52.
[155]王平,胡正嘉.棉花VA菌根真菌的分离鉴定[J].华中农业大学学报,1989,8(1):36-44.
[156]王发园,刘润进.丛枝菌根真菌一新种:枣庄球囊霉[J].菌物系统,2002,21(4):522-524.
[157]王幼珊,张美庆,王克宁,邢礼军.我国东南沿海地区的AM真菌Ⅳ.四个我国新记录种[J].菌物系统,1998,17(4):301-303.
[158]潘幸来,张贵云,王永杰,吴慎杰.黄土高原的VA菌根真菌Ⅳ[J].菌物系统,1997,16(3):166-168.
[159]潘幸来,张贵云,王永杰.黄土高原的VA菌根真菌(Ⅲ)—新纪录种橙棕色盾巨孢囊霉及其与异配盾巨孢囊霉的比较[J].山西大学学报,1996,19(2)187-190.
[160]潘幸来,张贵云,王永杰,吴慎杰.黄土高原的一个VA菌根真菌新种:三红盾巨孢囊霉[J].菌物系统,1997,16(3):169-171.
[161]赵之伟,杜刚.云南热带蕨类植物根际土壤中的六种VA菌根真菌[J].菌物系统,1997,16(3):208-211.
[162]赵之伟.云南热带,亚热带蕨类植物根际土壤中的VA菌根真菌[J].云南植物研究,1998,20(2):183-192.
[163]汪洪刚,吴观以,李慧全.一个我国内囊霉科实果内囊霉属新记录的种2弯丝波纹状实果内囊霉[J].真菌学报,1992,11(1):78-79.
[164]杨安娜,李凌飞,赵之伟.中国丛枝菌根真菌一新记录种[J].菌物学报,2004,23(4):603-604.
[165]乔红权,张英,郭良栋,傅俊范.新疆北部地区常见植物根围的丛枝菌根真菌[J].菌物学报,2005,24(1):130-136.
[166]Trappe J M.Fungus associates of ectatrophic mycorrhizae[J].Bot Rev,1962,28:538-606.
[167]Smith S E,Read D J.Mycorrhizal Symbiosis(2 nd Ed)[M].California:ACADEMIC PRESS,1997.
[168]郭秀珍,毕国昌.林木菌根及应用技术[M].北京:中国林业出版社,1989.
[169]谭方河,王云璋.四川松树,桉树外生菌根菌种类调查[J].四川林业科技,2000,21(3):65-69.
[170]陈辉,唐明.杨树菌根研究进展[J].林业科学,1997,33(2):181-188.
[171]Harley J L,Sm ith S E.Mycorrhizal Symbiosis[M].Academic Press,London,1983,1-483.
[172]Fisher P J,Pctrini O.Ecology,biodiversity and Physiology of endophytic fungi[J].Curr.Top.Bot.Res,1993,1:271-279.
[173]Hyde K D,D L Hawksworth.Measuring and monitoring the biodiversity of microfungi [A].In:K D Hyde.Biodiversity of Tropical Microfungi[M].Hong Kong:Hong Kong University Press,1997:11-28.
[174]McNeely J A.Strategies of conserving biodiversity[J].Environment.1990,32(3):16-42.
[175]施立明.遗传多样性及其保存[J].生物科学信息,1990,(2):159-164.
[176]沈浩,刘登义.遗传多样性概述[J].生物学杂志,2001(6):6-9.
[177]李运贤,李玉英,邢倩.植物多样性的分子生物学研究方法[J].南阳师范学院学报,2005,4(9):52-56.
[178]邱芳,伏建民,金德敏,王斌.遗传多样性的分子检测[J].生物多样性,1998,6(2):143-150.
[179]潘莹,赵桂仿.分子水平的遗传多样性及其测量方法[J].西北植物学报,1998,18(4):645-653.
[180]Mukai Y,Suyama Y,Tsuotura Y,Kawahara T.A liokage map for sugi(Gyptomeria japonica) based on RFLP,RAPD and isozyme foei[J].Theor Appl Genet,1995,90:835-840.
[181]Williams J G K,Kubelik A R,Livak J.DNA polymorphisms amplified by arbitary primers are useful as genetic markers[J].Nucleic Acid Research,1990,18:6531-6535.
[182]Welsh J,MmClelland M.Fingerprinting genomes using PCR with arbitary primers[J].Nucleic Acids Research,1990,18:7213-7218.
[183]任旭琴.遗传多样性及其研究方法[J].淮阴工学学报,2002,11(5):6-7.
[184]谢佳燕,张知彬.ISSR技术及其在遗传多样性研究中的应用[J].兽类学报,2004,24(1):71-76.
[185]Zabeau M,Vos P.Selective restriction fragment amplification:a general method for DNA fin gerprinting[P].European Patent Application No:0534858,1993:
[186]李璇,王晓东.AFLP的研究[J].甘萧教育学院学报,2004,18(1):58-61.
[187]李伟,纪燕玲,于汉寿,莫凌霄,李飞凤.王志伟中国产禾本科植物内生真菌的遗传多样性及rDNA-ITS系统发育分析[J].菌物学报,2006,25(2):217-226.
[188]Christoph R,Gr(u|¨)nig Thomas N.Sieber.Molecular and phenotypic description of the widespread root symbiont Acephala applanata gen.et sp.nov.,formerly known as dark-septate endophyte Type 1[J].Mycologia,2005,97(3):628-640.
[189]Ajay Kumar,Pandey,Mang Jye Ger,Hsiang En Huang,Mei Kuen Yip,Jiqing Zeng,Teng-Yung Feng.Sweet pepper ferredoxin-like protein(pflp)gene as a novel selection marker for orchid transformation[J].Planta,2003.
[190]魏宇昆,高玉葆,李川,许华,任安芝.内蒙古中东部草原羽茅内生真菌的遗传多样性[J].植物生态学报,2006,30(4):640-649.
[191]Lappalainen J H,Yli Mattila T.Genetic diversity in Finland of the birch endophyte Genomonia setacea as determined by RAPD-PCR markers[J].Mycol Res,1999,103:328-332.
[192]Shahasi Yusuf Athman.Host-endophyte-pest interactions of endophytic Fusarium oxysporum antagonistic to Radopholus similis in banana(Musa spp)[D].Athman S Y:University of Pretoria,2006:
[193]Prosser J I.Molecular and functional diversity in soil micro-organisms[J].Plant and Soil,2002,244:9-17.
[194]Chelius M K,Triplett E W.Rapid detection of arbuscular mycorrhizae in roots and soil of an intensively managed turfgrass system by PCR amplification of small subunit rDNA [J].Mycorrhiza,1999,9:61-64.
[195]Tonin C,Vandenkoornhuyse P,Joner EJ.Assesssment of arbuscular mycorrhizal fungi diversity in the rhizosphere of Viola calami naria and effect of these fungi on heavy metal uptake by clove[J].Mycorrhiza,2001,10(4):161-168.
[196]Tonin C,Vandenkoornhuyse P,Joner E J.Effect of metallic trace on AM fungi diversity within roots of Trifolium subterraneum colonised with AM spores isolated from a polluted soil:application to soil remediation[A].In:Abstracts of 3rd International Conference on Mycorrhizas[C].2001.
[197]Ridgway K P,Marland L A,Young J P W.Molecualr diversity of mycorrhizal fungi on the roots of trees grown with contaminated soil inoculum[A].In:Abstracts of 3rd International Conference on Mycorrhizas[C].2001.
[198]Renker C,Kaldorf M,Buscot F.Structural diversity of arbuscular mycorrhizal fungi in a disturbed grassland[A].In:Abstracts of 3rd International Conference on Mycorrhizas[C].2001.
[199] Redecker D. Specific PCR primers to identify arbuscular mycorrhizal fungi within colonized roots [J] . Mycorrhiza, 2000,10: 73-80.
[200] Hijri M, Redecker D, Sanders I R. Scutellospora castanea spores as microbial communities [A] . In: Abstracts of 3rd International Conference on Mycorrhizas[C],2001.
[201] Versaw. W. K, Chiou. T, Harrison. M. J. Phosphate transporters of Medicago truncatula and arbuscular mycorrhizal fungi [J] . Plant and Soil, 2002,244: 239-245.
[202] Requena N, Mann P, Hampp R, Franken P. Early developmentally regulated genes in the arbuscular mycorrhizal fungus Glomus mosseae: identification of GmGINI, a novel gene with homology to the C-terminus of metazoan hedgehog proteins [J] . Plant and Soil,2002,224: 129-139.
[1]Guo L D,Hyde K D,Liew E C.A method to promote sporulation in palm endophytic fungi[J].Fungal Diversity,1998,1:109-113.
[2]王静,任交芝,谢凤行,魏宇昆,高玉葆.几种诱导黑麦草Lolium perenne L.内生真菌产孢的方法[J].菌物学报,2005,24(4):590-596.
[3]郭春秋,罗永兰,张志远.几种真菌的诱导产孢试验[J].海南大学学报自然科学版,2003,21(1):74-77.
[4]Bills G F,Polishook J D.Recovery of endophytic fungi from Chamaecyparis thyroids [J].Sydowia,1992,44:1-12.
[5]Gene J,Mercado-Sierra A,Guarro J.Dactylaria cazorlii and Hansfordia catalonica,two new hyphomycetes from litter in Spain[J].Mycol.Res.2000,104:1404-407.
[6]Kirk P M.New or interesting microfungi XV.Miscellaneous hyphomycetes from the British Isles[J].Trans.Br.Mycol.Soc.1986,86:409-428.
[1]郭顺星,徐锦堂.真菌在罗河石解和铁皮石解种子萌发中的作用[J].中国医学科学院学报,1991,13(1):46-47.
[2]吴智彪、宋希强、李绍鹏.华石斛内生真菌诱导子对其组培苗生长的影响[J].热带作物学报,2006,27(1):77-79.
[3]杨友联、刘作易、朱国胜、毛堂芬.独蒜兰菌根真菌对环草石斛生长的影响[J].种子,2008,27(1),34-37.
[4]魏景超.真菌鉴定手册[M].上海:科学出版社,1979.
[5]H L巴尼特,B.B.亨特.半知菌属图解[M].北京:科学出版社,1977.
[6]Ellis M B.Dematiaceous hyphomycetes.Ⅷ[J].Mycol.Pap.1967,111:29.
[7]Helgard I N,Kerry O'Donnell.New Fusarium species and combinations within the Gibberella fujikuroi species complex[J].Mycologia,1985,90(3):434-458.
[8]Emory G S.Alternaria themes and variations[J].Mycotaxon,1997,65(8):1-91.
[9]Emory G S.Alternaria themes and variations[J].Mycotaxon,1986,25(1):287-308.
[10]Gams W.Cephalosporium-like hyphomycetes:some tropical species[J].Trans.Br.Mycol.Soc.1975,64(3):389-404.
[11]Hermanides E Jf.Aureobasidium and allied genera[J].Stud.Mycol.1977,15:141-166.
[12]C.布斯.镰刀菌属[M].北京:北京大学出版社,1987.
[13]Stanley J.Hughes.Sooty moulds.Mycologia[J].1976,68(4):693-820.
[14]White J F,Morgan J.Endophyte-host association in forage grasses Ⅷ.A cremonium chilense,an undescribed endophyte occurring in dactylic glomerata in chile[J].Mycotaxon,1990,39:441-452.
[15]Helgard I Nirenberg,Kerry O'Donnell.New Fusarium species and combinations within the Gibberella fujikuroi species complex[J].Mycologia,1985,90(3):434-458.
[16]Castaneda R F,Kendrick B,Guarro J.Notes on conidial fungi.Ⅺ Ⅴ.New hyphomycetes from cuba[J].Mycotaxon,1997,65:93-105.
[17]Dowsett J A,Reid J,Hopkin A.On cephalosproium balanoides drechsler[J].Mycologia,1982,74(4):687-690.
[18]Niger L,Hywel J,Harry C E,Yan J.Are-evaluation of the leafhopper pathogen Torrubiella hemipterigena,its anamorph Verticillium hemipterigenum and V.pseudohemipterigenum sp.Nov[J].Mycological Research.,1997,101(10):1242-1246.
[19]Barron G L.New species and new records of fungi that attack microscopic animals[J].Can.J.Bot.,1989,67(1):267-271.
[20]Walter M.Jaklitsch,Gary J S,Sarah L D,Bing-Sheng Lu,Irina S D.Hypocrea rufa/Trichoderma viride:a reassessment,and description of five closely related species with and without warted conidia[J].Stud Mycol.,2006,56(1):135-177.
[21]David M.The genus thielavia[J].Mycologia,1973,65(5):1055-1077.
[22]Smalley E.B.,Hansen H N.The perfect stage of Gliocaldium roseum[J].Mycologia,1957,49:432-499.
[23]David J C.A contribution to the systematics of Cladosporium[J].Mycological papers,1997,172:25-31.
[24]叶琪明.中国南方镰孢属的研究Ⅱ:马特组Section Martiella的分类研究[J].浙江农业学报,2000,12(6):378-381.
[25]Hughes S J.Revisiones hyphomycetum aliquot cum appendice de nominibus rejiciendis[J].Canadian Journal of Botany.1958,36:727-836.
[26]Sutton B C.Microfungi on Xanthorrhoea[J].Transactions of the British Mycological Society 1980,(85):239-255.
[27]郭良栋.内生真菌研究进展[J].菌物系统,2001,20(1):148-152.
[28]Petrini O,Carrol G C.Endophytic fungi in folige of some Cupressaceae in Oregon[J].Canadian Journal of Botany.1981,59:629-636.
[29]Petrini O.Taxonomy of endophytic fungi of aerial plant tissues.In microbiology of the phyllosphere[M].Canbrige,Cambrige University Press,1986.
[30]范黎,郭顺星,徐锦堂.我国部分兰科植物菌根的内生真菌种类研究[J].山西大学学报,1998,21(2):169-177.
[31]张廷威,康冀川.石斛内生真菌的研究概述[J].山地农业生物学报,2005,24(5):438-441.
[32]张廷威.石斛茎部内生真菌的分离与分布[J].贵州农业科学,2007,35(2):18-19.
[1]Guo L D,Hyde K D & Liew E C Y.A method to promote sporulation in palm endophytic fungi[J].Fungal Diversity,1998,1:105-109.
[2]Guo L D,Hyde K D & Liew E C Y.Identification of endophytic fungi from Livistona chinensis(Palmae) using morphological and molecular techniques[J].New Phytologist,2000,147:617-630.
[3]Pandey A K,Reddy M S,Suryanarayanan T S.ITS-RFLP and ITS sequence analysis of a foliar endophytic Phyllosticta from different tropical trees[J].Mycol Res,2003,107:439-444.
[4]Thompson J D,Gibson T J,Plewnicak F,Higgins.D G.The ClustalX windows interface:flexible strategies for myltiple sequence alignment aided by quality analysis tools[J].Nucleic Acids Research,1997,24:4876-4882.
[5]范黎,郭顺星,徐锦堂.我国部分兰科植物菌根的内生真菌种类研究[J].山西大学学报,1998,21(2):169-177.
[6]张廷威、康冀川.石斛内生真菌的研究概述[J].山地农业生物学报,2005,24(5):438-441.
[7]Murali T S,Suryanarayanan TS,Venkatesan G.Fungal endophyte communities in two tropical forests of southern India:diversity and host affiliation[J].Mycol.Progress,2007,6:191-199.
[8]Thomas J,Bastian B,Gerhard G,Daniel B,Claire D,Marc-Andre S.Mixotrophy in orchids:insights from a comparative study of green individuals and onphotosynthetic individuals of Cephalanthera damasonium[J].New Phytologist,2005,166:639-653.