胡杨再生体系的建立及rolB-pttGA20ox基因的遗传转化研究
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摘要
本研究以胡杨当年生枝条的带芽茎段为外植体,首先建立了它的离体快繁及植株再生体系,并就植物生长调节剂等因素对胡杨叶片再生不定芽的影响进行了详细的研究;然后利用NPTⅡ筛选基因对影响根癌农杆菌介导胡杨遗传转化的条件进行了深入探索,通过优化转化条件,建立了胡杨遗传转化系统;最后在此基础上,进行rolB-pttGA20ox双价基因的转化,并由PCR、PCR-Southern杂交分子检测,验证rolB-pttGA20ox基因的插入情况。
研究结果摘要如下:
1.胡杨组培再生体系的建立
消毒的最佳方法为:夏季采集的材料: 70%的酒精浸泡30sec后,以0.1%氯化汞分别灭菌7min、8min;初春水培枝条:试剂同上,茎尖灭菌0.5min,茎段灭菌1.0min。最适宜的启动培养基为MS+6-BA0.5mg/L+NAA0.1mg/L,MS+6-BA0.5mg/L+NAA0.2mg/L+糖20 g/L为增殖培养的最佳配方,生根培养的最适培养基为1/2MS+IBA1.0mg/L+糖25g/L+琼脂6g/L+AC 0.2 g/L,生根率达到81%。移栽炼苗以蛭石:珍珠岩(2:1)或者沙子:土壤(1:1)作为最佳基质。
2.在组培中黄化现象产生的规律以及避免的方法
在一个继代周期内,随着时间的推移,试管苗的黄化率越来越高,整体呈现线性上升的趋势;经生理生化指标研究发现,当继代时间超过20d后,叶绿素含量尤其以叶绿素a的降幅较大,建议可以接种20d为一个继代周期;ABA为IAA、ZR的拮抗物,它的消长过程大体上与生长过程是相反的;采用SDS-聚丙烯酰胺凝胶电泳法对蛋白质进行分析,发现黄化试管苗电泳谱带均出现缺失等异常现象。这说明黄化苗在基因表达调控上出现差异,其内部机制可能已发生变化。研究影响胡杨黄化率的培养条件因子发现:培养基的pH值在6.0～7.0之间、昼夜变温处理、不同波段的光质中以蓝光对降低胡杨黄化率有较好效果。
3.胡杨遗传转化体系的建立
胡杨叶片再生的最适培养基是MS+BA0.5mg/L+NAA0.1～0.2mg/L+腺嘌呤40mg/L+水解乳蛋白500mg/L +白砂糖25g/L+琼脂6.0g/L,再生频率达100%;叶片培养的最佳取材位置是自茎尖展叶始第1～3片叶;叶片正面向上或正面向下接种培养,对其
The major objective of this study was to breed new Populus euphratica varieties with improved rooting ability and apical dominance through transformation of the rolB-pttGA20ox two genes by using Agrobacterium tumefaciens-mediate transformation technology. For this purpose, first, an efficient in vitro propagation system and plant regeneration system were developed for Populus euphratica. Factors that affecting shoots regeneration via plant growth regulators etc., were investigated in details. Subsequently, parameters that affecting the efficiency of Agrobacterium tumefaciens-mediate transformation of Populus euphratica were comprehensively studied and optimized by accessing NPTⅡexpression, and a transformation protocol was established. Finally, on the basis of the established transformation system, pttGA20ox、rolB-pttGA20ox genes were transferred to Populus euphratica by PCR and PCR-Southern blotting tests.
The results of this study were introduced separately as following:
1. Establishment of plant egeneration system of Populus euphratica
The optimum sterilized measure for summer material is to be immerged in 70% ethanol for 30seconds and then in 0.1% HgCl2 for 7 and 8 minutes in succession. For stem apexes, it’s better to be immerged in 70% ethanol for 30seconds and then in 0.1% HgCl2 for 0.5 minutes, but for stem segments with axillary buds the optimum time in 0.1% HgCl2 is 1.0 minutes. The optimal induction medium was MS+6-BA0.5mg/L+NAA0.1mg/L, the proliferation medium was MS+6-BA1.0mg/L+NAA0.2mg/L+sugar20g/L, and the most optimal rooting medium was 1/2MS+IBA1.0mg/L+surar 25g/L+AC0.2g/L, rooting rate being up to 81%. And the appropariate substance for seeding transplantation is vermiculite and perlite in 2:1 or sand and soil in 1:1.
2. Etiolation in the vitro of Populus euphratica.and measures:
The etiolated rate was increased with the increasing of days obviously within a subculture circle. With some index of physio-biochemical the results showed that: when the subculture time was more than 20 days, chlorophyll content falled much especially
研究结果摘要如下:
1.胡杨组培再生体系的建立
消毒的最佳方法为:夏季采集的材料: 70%的酒精浸泡30sec后,以0.1%氯化汞分别灭菌7min、8min;初春水培枝条:试剂同上,茎尖灭菌0.5min,茎段灭菌1.0min。最适宜的启动培养基为MS+6-BA0.5mg/L+NAA0.1mg/L,MS+6-BA0.5mg/L+NAA0.2mg/L+糖20 g/L为增殖培养的最佳配方,生根培养的最适培养基为1/2MS+IBA1.0mg/L+糖25g/L+琼脂6g/L+AC 0.2 g/L,生根率达到81%。移栽炼苗以蛭石:珍珠岩(2:1)或者沙子:土壤(1:1)作为最佳基质。
2.在组培中黄化现象产生的规律以及避免的方法
在一个继代周期内,随着时间的推移,试管苗的黄化率越来越高,整体呈现线性上升的趋势;经生理生化指标研究发现,当继代时间超过20d后,叶绿素含量尤其以叶绿素a的降幅较大,建议可以接种20d为一个继代周期;ABA为IAA、ZR的拮抗物,它的消长过程大体上与生长过程是相反的;采用SDS-聚丙烯酰胺凝胶电泳法对蛋白质进行分析,发现黄化试管苗电泳谱带均出现缺失等异常现象。这说明黄化苗在基因表达调控上出现差异,其内部机制可能已发生变化。研究影响胡杨黄化率的培养条件因子发现:培养基的pH值在6.0～7.0之间、昼夜变温处理、不同波段的光质中以蓝光对降低胡杨黄化率有较好效果。
3.胡杨遗传转化体系的建立
胡杨叶片再生的最适培养基是MS+BA0.5mg/L+NAA0.1～0.2mg/L+腺嘌呤40mg/L+水解乳蛋白500mg/L +白砂糖25g/L+琼脂6.0g/L,再生频率达100%;叶片培养的最佳取材位置是自茎尖展叶始第1～3片叶;叶片正面向上或正面向下接种培养,对其
The major objective of this study was to breed new Populus euphratica varieties with improved rooting ability and apical dominance through transformation of the rolB-pttGA20ox two genes by using Agrobacterium tumefaciens-mediate transformation technology. For this purpose, first, an efficient in vitro propagation system and plant regeneration system were developed for Populus euphratica. Factors that affecting shoots regeneration via plant growth regulators etc., were investigated in details. Subsequently, parameters that affecting the efficiency of Agrobacterium tumefaciens-mediate transformation of Populus euphratica were comprehensively studied and optimized by accessing NPTⅡexpression, and a transformation protocol was established. Finally, on the basis of the established transformation system, pttGA20ox、rolB-pttGA20ox genes were transferred to Populus euphratica by PCR and PCR-Southern blotting tests.
The results of this study were introduced separately as following:
1. Establishment of plant egeneration system of Populus euphratica
The optimum sterilized measure for summer material is to be immerged in 70% ethanol for 30seconds and then in 0.1% HgCl2 for 7 and 8 minutes in succession. For stem apexes, it’s better to be immerged in 70% ethanol for 30seconds and then in 0.1% HgCl2 for 0.5 minutes, but for stem segments with axillary buds the optimum time in 0.1% HgCl2 is 1.0 minutes. The optimal induction medium was MS+6-BA0.5mg/L+NAA0.1mg/L, the proliferation medium was MS+6-BA1.0mg/L+NAA0.2mg/L+sugar20g/L, and the most optimal rooting medium was 1/2MS+IBA1.0mg/L+surar 25g/L+AC0.2g/L, rooting rate being up to 81%. And the appropariate substance for seeding transplantation is vermiculite and perlite in 2:1 or sand and soil in 1:1.
2. Etiolation in the vitro of Populus euphratica.and measures:
The etiolated rate was increased with the increasing of days obviously within a subculture circle. With some index of physio-biochemical the results showed that: when the subculture time was more than 20 days, chlorophyll content falled much especially
引文
1. 卜学贤,陈维伦.活性炭对培养基中植物生长调节物质的吸附作用[J].植物生理学报,1988,14(4):401-405
2. 步怀宇,景建洲,贾敬芬. 发根农杆菌对骆驼刺的转化及转化组织的形态发生[J]. 实验生物学报,2001
3. 曹孜义,刘国民 主编.实用植物组织培养技术教程[M].兰州:甘肃科学技术出版社,1999
4. 陈福明, 陈顺伟. 混合液法测定叶绿素含量的研究[J].林业科技通讯,1984,(2):4-8
5. 陈婉芬著.植物生长调节物质及其应用[M].南京:江苏科学技术出版社,1988
6. 陈维纶,陶国清等 编著.植物生物技术[M].北京:科学出版社,1987
7. 陈永勤,胡传银. 怀地黄不定芽诱导生根和微型块茎繁殖的研究[J]. 湖北大学学报(自然科学版), 2004,24(1):61-64
8. 程家胜,史永忠,张志云等. 苹果组织培养中的玻璃化苗问题. 植物生理学通讯,1990, 26(1):33-35
9. 达克东,张松,米瑞芙等. 创伤诱导苹果离体叶片高效直接体细胞胚胎发生[J]. 核农学报, 2001,15(5):290-293
10. 丁霞,陈晓阳,李云等.胡杨叶片不定芽再生体系的研究[J].北京林业大学学报,2003,25(2):28-31
11. 杜雄明,陈金桂,张天真,潘家驹,汪若梅.5 个棉花纤维突变体胚珠内源激素差异及对纤维分化和前期生长的影响[J].棉花学报,1998,10(1):43-51.
12. 樊军锋,李玲,韩一凡等. 84K 杨叶片外植体再生系统的建立[J]. 西北林学院学报, 2002,17(2):33-36
13. 范小峰,李师翁,张民权. 河北杨组织培养快繁技术研究. 中国水土保持,2001,(3): 17-18
14. 谷瑞升,蒋湘宁.胡杨离体器官发生及试管无性系的建立[J].植物学报,1999,41(1):29-66.
15. 谷瑞升,刘群录,陈雪梅等. 木本植物蛋白提取和 SDS-PAGE 分析方法的比较和优化[J]. 植物学通报,1999,21(5):7-10
16. 管志文,张清杰,庄楚雄,等.农杆菌携带柞蚕抗菌肽基因转入桑树的研究[J].蚕业科学,1994,20(1):1-6.
17. 桂耀林,马诚 编著.植物组织培养[M]. 北京:科学出版社,1985
18. 郝贵霞,朱桢,朱之梯.毛白杨叶片组培再生芽的玻璃化问题探讨[J]. 北京林业大学学报,1999,21(1):68-71
19. 郝贵霞,朱祯,朱之悌.毛白杨遗传转化系统优化的研究[J].植物学报,1999,41:936-940.
20. 郝贵霞.毛白杨转化条件的优化及抗虫基因植株的鉴定[D].北京:北京林业大学,1998.
21. 郝俊. 环境因子对胡杨苗木高生长影响的相关分析. 林业科技,1991,16(1):18-20
22. 何钟佩著.农作物化学控制实验指导[M].北京:北京农业大学出版社,1993
23. 黄学林,李莜菊. 高等植物组织离体培养的形态建成及其调控[M]. 科学出版社,1995
24. 贾春兰,王纪方,杨健荣等.葡萄试管苗繁殖技术研究[J].中国果树,1992(1):25.
25. 贾乃光 主编.数理统计(第 2 版)[M].北京:中国林业出版社,1993
26. 姜静主编. 细胞生物学实验原理与技术[M]. 哈尔滨:东北林业大学出版社,2004
27. 金方伦. 贵州遵义柑桔黄蜘蛛的防治[J]. 植物医生, 1999,12(4):18
28. 兰彦平,顾万春.林木无性繁殖研究进展[J].世界林业研究,2002,15(6):7-13.
29. 李合生主编. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000
30. 李洪清,李美茹.影响农杆菌介导植物基因转化的因素问题[J].植物生理学通讯,1999, 35:145-151.
31. 李家书,陈防,吕忠贵等. 长江三峡地区柑桔施用螯合铁肥效果[J]. 湖北农业科学,1997,2:25-27
32. 李浚明 编译.植物组织培养教程[M].北京:中国农业大学出版社,1998
33. 李玲,齐力旺,韩一凡.TA29-Barnase 基因导致抗虫转基因欧洲黑样雄性不育的研究[J].林业科学,2000,36(1):28-32.
34. 李雅丽,张凯. 用农杆菌 Ri 诱导蒙古黄芪发根培养的研究[J].云南植物研究,2005,27(2):204-210
35. 李伟.转双价基因促进植物生根及顶端优势的研究[D].北京林业大学,2004.
36. 李云主编.林果花菜组织培养快速育苗技术[M].中国林业出版社,2001
37. 梁机. 转 rolB 基因改良毛白杨生根能力的研究 2004[D]..北京:北京林业大学图书馆,2004
38. 梁玉堂,龙庄如.树木营养繁殖原理和技术[M].北京:中国林业出版社,1993.
39. 林静芳,董茂山. 白杨派树种的组织培养[J]. 林业科学,1980,16(增刊):58-64
40. 刘传飞,于树宏,李玲等. 发根土壤杆菌对葛属药用植物的遗传转化[J]. 植物学报, 2000,42(9):936-939
41. 刘桂丰,杨传平,刘关君等.盐逆境条件下三个树种的内源激素变化[J].东北林业大学学报,1998,26(1):1-3
42. 刘康,孙敬,张天真,潘家驹.内源及外源植物激素对陆地棉无絮突变体胚珠纤维初始发育诱导效应的研究[J].棉花学报,1999,11(1):48-56.
43. 刘清丽,李合生,伍素辉. 光质对农垦 58s 黄化苗形态建成、色素和蛋白质含量的影响[J]. 华中农业大学学报,1994,13(6):636-640
44. 刘晓青,周建涛. 外源 GA_3 对春石斛园艺性状的影响[J]. 江苏农业科学, 2004(5):77
45. 刘用生,李友勇.植物组织培养中活性炭的使用[J].植物生理学通讯,1994,30(3):217—241.
46. 陆万香,李名扬,裴炎等. 玉米几丁质酶基因导入甘蓝型油菜的研究[J]. 西南农业大学学报,2001,23(2):130-133
47. 罗晓芳等.金丝小枣组织培养快速繁殖的研究[J].北京林业大学学报.1996,18(2):9-15.
48. 倪德祥,冯文煦,张丕方等. 光质对一品红叶离体培养中形态发生及生化变化的影响[J]. 广西植物,1987,7(4):339-345
49. 潘磊庆,屠康,贾明敏等.臭氧水处理对芹菜保鲜效果的研究[J]. 安徽农业大学学报,2004,31(3):348-352
50. 潘瑞炽,董愚得.植物生理学[M].北京:高等教育出版社,1995
51. 潘瑞炽.植物组织培养[M].广东:广东高等教育出版社,2001.
52. 彭方仁.无性繁殖的进展及其在无性系林业中应用前景[J].世界林业研究,1990,3(4):74-80.
53. 卜学贤,陈维纶. 试管植物的玻璃化现象. 植物生理学通讯,1987,23(5):13-9
54. 祁春芳,郑智礼. 白杨派杨树组织培养技术研究. 山西林业科技,2000,(4):21-23
55. 齐燕红. 毛白杨快繁及玻璃化研究[D]. 北京:北京林业大学图书馆,1993
56. 沈效东,张新宁,袁世杰等. 生长调节剂对毛白杨叶愈伤组织诱导和植株再生的影响[J]. 宁夏农林科技,1996,(6):18-21
57. 施和平,李玲,潘瑞炽. 乙酰丁香酮对发根农杆菌遗传转化黄瓜的影[J]. 云南植物研究, 1997,19(4):445-448
58. 孙敬三,桂耀林 主编.植物细胞工程实验技术[M]. 北京:科学出版社,1995
59. 孙雪新,陈红.胡杨组织培养.植物学通报[J],1992,9(增刊):49-50.
60. 孙雪新,康向阳,李毅等. 胡杨的研究现状及发展建议[J]. 世界林业研究,1993,(4): 48-52
61. 孙雪新,康向阳.胡杨无性繁殖研究[J],甘肃林业科技,1993(1):27-30.
62. 谭文澄,戴策刚.观赏植物组织培养技术[M].中国林业出版社,1991.
63. 汪志军,李康,处格鲁克·艾克等.胡杨的组织培养[J].新疆农业科学,1997(4),185-186.
64. 王关林,方宏筠 主编.植物基因工程原理与技术[M].北京:科学出版社,1998.
65. 王敬驹,匡柏健,曾慧.提高甘蔗组织培养效率的研究[J].植物学通报,1983,1(2):17
66. 王明庥.林木遗传育种学[M].北京:中国林业出版社,2001.4.
67. 王善平,许农,许智宏.利用 PEG 法对 GUS 基因在几种杨树原生质体中瞬间表达的研究[J].试验生物学报,1991,24:71-73.
68. 王世绩. 全球胡杨林的现状及保护和恢复对策[J]. 世界林业研究,1996,(6):37-44
69. 王影,黄敏仁,陈道明等. 杨树细胞悬浮培养及体细胞胚胎发生的研究[J]. 南京林业大学学报,1991,15(3):31-36
70. 王勇.抗生素对桑树外植体生长与分化的影响[J].蚕业科学,1996,22(2):72-76.
71. 魏庆主编.胡杨[M].北京.中国林业出版社,1998. 104-141.
72. 吴颂如.酶联免疫法测定植物内源激素[J].植物生理学通讯,1988,(5):53-56
73. 吴延军.桃组织培养及遗传转化的研究[D].杭州:浙江大学图书馆,2004
74. 夏阳,梁慧敏,孙仲序等. 四倍体刺槐转甜菜碱醛脱氢酶基因的研究[J]. 山东林业科技,2003,(3):1-3
75. 徐妙珍等,林木组织培养的障碍因素及对策(一)[J],林业科技.1991,16(4):5-6
76. 徐妙珍,张雅君,李伟等. 林木组织培养的障碍因素及其对策. 林业科技,1992,17(3): 10-12
77. 徐纬英.杨树选种学[M].科学出版社,1960
78. 续九如,黄智慧 编著.林业试验设计[M].北京:林业出版社,1995
79. 杨秀荣,陈永文,方平. 乙酰丁香酮对根癌农杆菌介导的甘薯遗传转化的影响[J]. 西南师范大学学报(自然科学版),2002,27(5):52-56
80. 尹淑萍,草莓栽培品种离体再生及遗传转化的研究[D].中国农业大学,2004
81. 于建云. 散射光、变温处理对香石竹玻璃苗的影响研究[J].华东森林经理,2002,16(4):27-28
82. 陆万香,李名扬,裴炎等. 玉米几丁质酶基因导入甘蓝型油菜的研究[J]. 西南农业大学学报 2001,23(2):130-133
83. 詹亚光.杨树花药培养筛选耐盐变异体的研究[J].植物研究,1994,14(1):98-103.
84. 张克,郁东宁.胡杨茎尖组织培养的初步研究[J].宁夏农林科技,1989(5):25-26.
85. 张丕方,倪德祥,包慈华编著. 木本植物组织培养与繁殖上的应用. 上海:上海教育出版社,1985
86. 张平冬,康向阳,高鹏,韩锦. 胡杨离体培养分化增殖途径的比较研究[J].北京林业大学学报,2003,25(6):50-54
87. 张平冬. 胡杨无性繁殖与优树选择研究[D].北京林业大学,2004.
88. 张卫芳,高疆生,段黄金等. 胡杨离体快繁技术研究[J]. 新疆农业科学,2001,38(6): 320-322
89. 章镇,孙爱君,房经贵,盛炳成.农杆菌介导 rolC 基因转化烟草植株的研究[J].南京农业大学学报,2001,24(1):25-29.
90. 赵华燕,卢善发,晁瑞堂.杨树的组织培养及其基因工程研究[J].广西植物,2001,18(2):169-176.
91. 郑丽.根癌农杆菌介导 SAG12-ipt 和 3DN-iaaL 基因转化切花菊及抗早衰研究[D].西南农业大学,2003
92. 周立刚,杨崇仁,王君健.露水草毛状根的诱导和培养[J].云南植物研究,1996,18(3): 336-340.
93. 周林元,桂耀林. 植物学集刊.离体培养下胡杨的器官发生和小植株形成[C].中国科学院植物研究所,科学 1983, 1(1)
94. 朱大保.国外杨树组培微繁技术的进展[J].北京林业大学学报,1990,12(1):84-91.
95. 朱广廉,植物组培中的外值体灭菌[J],生理学通讯.1996,32(6):444-449.
96. 朱建华,彭士勇 编著. 植物组织培养实用技术[M]. 北京:中国计量出版社,2001
97. 诸葛强,王婕琛,陈英等.新疆杨高效遗传转化系统的建立[J].植物资源与环境学报,2003,12(4):6-10.
98. 张桂花 王洪梅 王连祥牡丹组织培养技术研究[J]. 山东农业科学, 2001,(5):16-18
99. Arnold Sara Von, Induction and development of adventitious bud Primordia On embryos, buds and needles of Norway spruce (Picea abies (L.) Karst.) grown In Vitro, Institute of Physiologicl Botany University of Uppsala, Sweden, 1979,5(11):1-32
100.Barbare M.Reed,Jessica Mentzer,Piyarak Tamprasert&Xiao Lingyu,intenal bacterial contam inatio of micropropogated hazelnut: identification and antibiotic treatment [J], Plant cell, Tissue and organ culture.1998(52):67-70.
101.Berbee FM,et al. Induction of callus and tree from stem tip cultures of a hybrid poplar [J]. In Vitro,1972,7,269.
102.Brand R. The formation of adventitious organs Ⅱ.The origin of buds formed on young adventitious roots of Populous nigra L. ‘Italica’ [J]. Acta. Bot. Neerl.,1973,22(4):399-406.
103.Cheong EunJu; Moon Heung KYu. Effect of light, cytokinins and explant’s position on adventitious bud induction from the leaf segments of P. euphratica [J] (FRI) Journal of Forest Science (Seoul) (1999).No.62,92-98
104.Chupeau M.C , Pautot V , Chupeau Y. Recovery of transgenic tree safter electroporation of poplar protoplasts [J]. Transgenic Res.,1994,3:13-19.
105.Costantino P , Capone I , Cardarelli M , De Poalis A , Mauro ML , Trovato M. Bacterial plant oncogenes:the rol genes saga[J]. Genetica,1994,94:203-211.
106.D’halluin K, Bonne E, Bossut M et al. Transgenic maize plants by tissue electroplation. Plant Cell[J],1992,4:1495-1505
107.Debergh P , Aitken-christie J , Cohend , et al. Reconsideration of the term ‘vitrification’ as used in micropropagation [J]. Plant Cell Tiss. Org. Cult.,1992,30:135-140.
108.Estruch JJ,Chriqui D,Grossmann K,Schell J,Schmulling T.The plant oncogene rolC is responsible for the release of cytokinins from glucoside conjugates[J]. EMBO J,1991b,10(10):2889-2895.
109.Estruch JJ,Parets-Soler A,Schmulling T,Spena A.Cytosolic localization in transgenic plants of the rolC peptide from Agrobacterium rhizogenes[J]. Plant Mol. Biol.,1991c,17:547-550.
110.Gelvin SB. Crown gall disease and hairy root disease : A sledgehammer and a tackhammer [J]. Plant Physiol,1990,92:281-285.
111.Gu, R . S . , Jiang, X . N . , Guo,ZH . CH . Organogenesis and plantlet regeneration in vitro of Populus euphratica [J] . Acta Botanica Sinica. Jan.1999,41(1):29-33
112.Huang F H,Li X Y. Effects of concentration of acetosyringone and Agrobacterium tumefaciens on gus gene transformation efficiency of Populus[J]. In vitro Cell Dev. Biol.,1994,30A:67.
113.J . Washer, Differentation in Pinus radiata Callus Culture: The effect of nutrients [J]. New Zealand Journal of Forest Science, 1977,7(3):321-328
114.Jouanin L,Guerche P,Pamboukdjian N,Tourneur C,Casse-Delbart F,Tourneur J. Structure of T-DNA in plants regenerated from roots transformed by Agrobacterium rhizogenes strain A4[J].Mol Gen Genet,1987,206:387-392.
115.Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature,1970,227:680-685.
116.Leple JC , Miranda B , Michel MF et al. Transgenic poplars : expression of chimerical genes using four different constructs[J]. Plant Cell Reports,1992, 11:137-141.
117.Lledo M D, Crespo M B, Amomasco J B . The role of cytokinins and ethylene inhibitors on lenticel hypertrophy generation and ethylene production in vitro cultures of Populus euphratica Oliver [J]. Israel of Plant Science, 1995,43: 339-345
118.Ludlow M.M,Muchow RC.A critical evaluation of traits for improving crop yield in water-limited environments[J]. Advanced in Agronomy,1990,43:107-153.
119.Parsons TJ , Sinkar VP , Stettler RF et al.Transformation of poplar by Agrobacterium tumefaciens[J]. Bio/technology,1986,4(6):533-536.
120.Petit A,David C,Dahl GA,Ellis JG,Guyon P,Casse-Delbart F,T empe J. Further extension of the opine concept:plasmids in Agrobacterium rhizogenes cooperate for opine degradation[J]. Mol. Gen. Genet,1983,190:204-214.
121.Voskresenskaya N P, Drozdova T s, Krendeleva T E Efect of light quality on the organization of photosynthetic eletron transport chain of pea seedlings. Plant Physicl[J]. 1977,59:15
122.Renate Muller,Arne S.Ansderson,Mangrethe Serek. Ditterences in display life of minatare potted roses [J]. Scientia Horticulturne,1998,76:59-71.
123.Routn GR,Samantaray S,Mottley J,Das P. Biotechnology of the Rose:A review of recent progress[J]. Scientia Horticulturae,1990(81):201-228.
124.Salehi H , Khosh-khui MA. Simple procedure for disinfection of ‘baby masquerade’ miniature rose explants [J]. Scientia Horticulturne,1997,68: 145-148.
125.Schmulling T,Fladung M,Grossman K,Schell J. Hormonal content and sensitivity of transgenic tobacco and potato plants expressing single rol genes of Agrobacterium rhizogenes T-DNA [J]. Plant J.,1993,3:371-382.
126.Schmulling T,Schell J,Spena A. Promoters of the rolA, B and C genes of Agrobacterium rhizogenes are differentially regulated in transgenic plant[J]. Plant Cell,1989,1:665-670.
127.Sinnott E W. Plant morphogenesis [M].New York:McGraw-Hill,1960.
128.Slightom JL,Durand-Tardif M,Jouanin L,Tepfer D. Nucleotide sequence analysis of TL-DNA of Agrobacterium rhizogenes agropine type plasmid [J]. J. Biol. Chem.,1986,261:108-121.
129.Souq F.,coutos-thevenot P.,Yean H.,et al. Genetic transformation of roses,2 examples:one on morphogensis,the other on anther cyan biogynthe-tic pathway [J]. Acta. Hort.,1996 (424):381-388.
130.Turk S CH J, Melchers L S Den Dulk-Ras H et al. Environmental conditions differentially affect vir gene induction in different Agrobacterium strains. Role of the vir A sensor protein. Plant Mol Biol[J], 1991, 16:1051-1059
131.Tzfira T,Vinnocur B,Altman A,et al. rol-transgenic Poplus tremula:root development,root-bore bud regeneration and in vitro propagation efficiency[J]. Trees,1998,12:464-471.
132.Van der Salm TPM,Hanisch ten Cate CH, Dons HJM. Prospects for application ofrol genes for crop improvenment. Plant Mol Biol Rep. 1996,14(3):207-228
133.Van der Salm TPM,Bouwer R,Van Dijk A J,Keizer LCP,Hanisch ten Cate ChH,Van der Plas LHW,Dons JJM. Stimulation of scion bud release by rol gene transformed rootstocks of Rosa hybrida L [J]. J. Exp. Bot.,1998,49 (322):847-852.
134.Vardi A, Bleichmans, Aviv D. Genetic transformation of transgenic plant [J]. Plant Science,1990,69:199-206
135.Voskresenskaya N P, Drozdova T s, Krendeleva T E Efect of light quality on the organization of photosynthetic eletron transport chain of pea seedlings [J]. Plant Physicl. 1977,59:15
136.Watanabe M, Pawlicki N, Holefors A, et al. Genetic transformation of apple rootstock M26 with rolB gene and its influence on rooting. J Plant Physiol, 1998,53:371-380
137.Watanabe S, Kihuna K, Ide Y, Sasaki S. Establishment of a tissue culture system of Populus euphratica oliv [J]. Bulltin of the Tokyo University Forests, 1999,102:87-92
138.White FF,Taylor BH,Huffman GA,Gordon MP,Nester EW. Molecular and genetic analysis of the transformed DNA regions of root-inducing plasmid of Agrobacterium rhizogenes [J]. J Bacteriol. 1985,164:33-34.
139.Winton L. Tissue culture propagation of European aspen [J]. Forest Sci.,1971, 17 (3);348-350.
140.Ziv M , Ariel T. Vitrification in relation to stomatal deformation and malfunction in carnation leaves in vitro [M].Growth and Development of Micropropagated Plants. Kluwer Academic Press,Dordrecht,1994:143-154.
141.Bleecker AB,Schutte JL, Kende H. Antomical analysis of growth and development patterns in the internode of deeper water rice [J]. Planta,1986,169:490-497.
142.Cho HT , Kende H. Expression of expansin genes is correlated with growth in deepwater rice[J]. Plant cell,1997,9:1661-1667.
143.Cosgrove DJ. Loosening of plant cell wall by expansinse [J]. Nature,2000, 407:321-324.
144.Davis PJ. Plant hormones:physiology,biochemistry and molecular biology[M].. Dordrecht. The Netherlands:Kluwer academic publishers.1995,13-38.
145.Duckett CM , Lloyd CW. Gibberellic acid-induced microtubule reorientation in dwarf peas is accompanied by rapid modification of an α-tubulin isotype [J]. Plant J.,1994,5:363-372.
146.Fabian T,Lorbiecke R,Umeda M,Sauter M. The cell cycle genes cycA1-1 andcdc2Os-3 are coordinately regulated by gibberellin in planta [J]. Planta ,2000,211:376-383.
147.Giddings TH , Staehelin LA.Microtubule-mediated control of microfibril deposition:a re-examination of the hypothesis[M]. In The Cytoskeletal Basis of Plant Growth and Form,Academic Press,London,1991,85-99.
148.Hea PM , Yoshihito S , Makiko C , Paul KJ , Isomaro Y.CsAGP1 , a gibberellin-responsive gene from cucumber hypocotyls,edcodes a classical arabinogalactan protein and is involved in stem elongation[J]. Plant Physiol,2003,131(3):1450-1459.
149.Kurioka Y , Suzuki Y , Kamada H , Harada H. Promotion of flowering and morphological alterations in Atropa belladonna transformed with a CaMV 35S-rolC chimeric gene of the Ri plasmid [J]. Plant Cell Rep,1992,12:1-6.
150.Lorbiecke R , Sauter M. Induction of cell grown and cell division in the intercalary meristem of submerged deepwater rice[J].Planta,1988,204:140-145.
151.Mizuno R.Inhibition of gibberellin-induced elongation , reorientation of cortical microtubules and change of isoform of tubulin in epicotyl segments of Azuki bean by protein kinase inhibitors[J]. Plant Cell Physiol,1994,35:1149-1157.
152.Olszewski N,Sun TP,Guble F. American Society of plant biologists gibberellin signaling : biosynthesis , catabolism , and response pathways[J]. Plant Cell , 202,S61-S80.
153.Oono Y,Suzuki T,Toki S,Uchimiya H. Effects of the over-expression of the rolC gene on leaf development in transgenic periclinal chimeric plants [J]. Plant Cell Physiol,1993,34:745-752.
154.Shibaoka H. Plant hormone-induced changes in the orientation of cortical microtubules [J]. Plant Mol. Biol.,1994,45:527-544.
155.Uozu S , Tanaka-Ueguchi M , Kitano H , Hattori K , M Characterization of XET-ralated genes of rice[J]. Plant physiol,2000,122:853-859.
156.Van der Knaap,Jeong Hoe Kim,Hans Kende. A novel gibberellin-induced gene from rice and its potential regulatory role in stem growth [J]. Plant Physiol ,2000,122:695-704.
157.Yang GX,Jan A,Shen SH,Yazaki J,Ishikaw M a,Shimatani Z,Kishimoto N,Kikuchi S,Matsumoto H,Komatsu S. Microarray analysis of brassinosteroida and gibberellin-regulated gene expression in rice seedlings [J]. Mol. Genet Genomics,2004,271 (4):468-478.
158.Yaxley JR,Ross JJ,Sherriff LJ,Reid JB. Gibberellin biosynthesis mutationsand root development in pea [J]. Plant Physiol,2001,125:622-633.
159.Yi L,Hans K. Expression of alpha-expansin and expansin-like genes in deepwater rice [J]. Plant Physiology,2002,130(3):1396-1405.
2. 步怀宇,景建洲,贾敬芬. 发根农杆菌对骆驼刺的转化及转化组织的形态发生[J]. 实验生物学报,2001
3. 曹孜义,刘国民 主编.实用植物组织培养技术教程[M].兰州:甘肃科学技术出版社,1999
4. 陈福明, 陈顺伟. 混合液法测定叶绿素含量的研究[J].林业科技通讯,1984,(2):4-8
5. 陈婉芬著.植物生长调节物质及其应用[M].南京:江苏科学技术出版社,1988
6. 陈维纶,陶国清等 编著.植物生物技术[M].北京:科学出版社,1987
7. 陈永勤,胡传银. 怀地黄不定芽诱导生根和微型块茎繁殖的研究[J]. 湖北大学学报(自然科学版), 2004,24(1):61-64
8. 程家胜,史永忠,张志云等. 苹果组织培养中的玻璃化苗问题. 植物生理学通讯,1990, 26(1):33-35
9. 达克东,张松,米瑞芙等. 创伤诱导苹果离体叶片高效直接体细胞胚胎发生[J]. 核农学报, 2001,15(5):290-293
10. 丁霞,陈晓阳,李云等.胡杨叶片不定芽再生体系的研究[J].北京林业大学学报,2003,25(2):28-31
11. 杜雄明,陈金桂,张天真,潘家驹,汪若梅.5 个棉花纤维突变体胚珠内源激素差异及对纤维分化和前期生长的影响[J].棉花学报,1998,10(1):43-51.
12. 樊军锋,李玲,韩一凡等. 84K 杨叶片外植体再生系统的建立[J]. 西北林学院学报, 2002,17(2):33-36
13. 范小峰,李师翁,张民权. 河北杨组织培养快繁技术研究. 中国水土保持,2001,(3): 17-18
14. 谷瑞升,蒋湘宁.胡杨离体器官发生及试管无性系的建立[J].植物学报,1999,41(1):29-66.
15. 谷瑞升,刘群录,陈雪梅等. 木本植物蛋白提取和 SDS-PAGE 分析方法的比较和优化[J]. 植物学通报,1999,21(5):7-10
16. 管志文,张清杰,庄楚雄,等.农杆菌携带柞蚕抗菌肽基因转入桑树的研究[J].蚕业科学,1994,20(1):1-6.
17. 桂耀林,马诚 编著.植物组织培养[M]. 北京:科学出版社,1985
18. 郝贵霞,朱桢,朱之梯.毛白杨叶片组培再生芽的玻璃化问题探讨[J]. 北京林业大学学报,1999,21(1):68-71
19. 郝贵霞,朱祯,朱之悌.毛白杨遗传转化系统优化的研究[J].植物学报,1999,41:936-940.
20. 郝贵霞.毛白杨转化条件的优化及抗虫基因植株的鉴定[D].北京:北京林业大学,1998.
21. 郝俊. 环境因子对胡杨苗木高生长影响的相关分析. 林业科技,1991,16(1):18-20
22. 何钟佩著.农作物化学控制实验指导[M].北京:北京农业大学出版社,1993
23. 黄学林,李莜菊. 高等植物组织离体培养的形态建成及其调控[M]. 科学出版社,1995
24. 贾春兰,王纪方,杨健荣等.葡萄试管苗繁殖技术研究[J].中国果树,1992(1):25.
25. 贾乃光 主编.数理统计(第 2 版)[M].北京:中国林业出版社,1993
26. 姜静主编. 细胞生物学实验原理与技术[M]. 哈尔滨:东北林业大学出版社,2004
27. 金方伦. 贵州遵义柑桔黄蜘蛛的防治[J]. 植物医生, 1999,12(4):18
28. 兰彦平,顾万春.林木无性繁殖研究进展[J].世界林业研究,2002,15(6):7-13.
29. 李合生主编. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000
30. 李洪清,李美茹.影响农杆菌介导植物基因转化的因素问题[J].植物生理学通讯,1999, 35:145-151.
31. 李家书,陈防,吕忠贵等. 长江三峡地区柑桔施用螯合铁肥效果[J]. 湖北农业科学,1997,2:25-27
32. 李浚明 编译.植物组织培养教程[M].北京:中国农业大学出版社,1998
33. 李玲,齐力旺,韩一凡.TA29-Barnase 基因导致抗虫转基因欧洲黑样雄性不育的研究[J].林业科学,2000,36(1):28-32.
34. 李雅丽,张凯. 用农杆菌 Ri 诱导蒙古黄芪发根培养的研究[J].云南植物研究,2005,27(2):204-210
35. 李伟.转双价基因促进植物生根及顶端优势的研究[D].北京林业大学,2004.
36. 李云主编.林果花菜组织培养快速育苗技术[M].中国林业出版社,2001
37. 梁机. 转 rolB 基因改良毛白杨生根能力的研究 2004[D]..北京:北京林业大学图书馆,2004
38. 梁玉堂,龙庄如.树木营养繁殖原理和技术[M].北京:中国林业出版社,1993.
39. 林静芳,董茂山. 白杨派树种的组织培养[J]. 林业科学,1980,16(增刊):58-64
40. 刘传飞,于树宏,李玲等. 发根土壤杆菌对葛属药用植物的遗传转化[J]. 植物学报, 2000,42(9):936-939
41. 刘桂丰,杨传平,刘关君等.盐逆境条件下三个树种的内源激素变化[J].东北林业大学学报,1998,26(1):1-3
42. 刘康,孙敬,张天真,潘家驹.内源及外源植物激素对陆地棉无絮突变体胚珠纤维初始发育诱导效应的研究[J].棉花学报,1999,11(1):48-56.
43. 刘清丽,李合生,伍素辉. 光质对农垦 58s 黄化苗形态建成、色素和蛋白质含量的影响[J]. 华中农业大学学报,1994,13(6):636-640
44. 刘晓青,周建涛. 外源 GA_3 对春石斛园艺性状的影响[J]. 江苏农业科学, 2004(5):77
45. 刘用生,李友勇.植物组织培养中活性炭的使用[J].植物生理学通讯,1994,30(3):217—241.
46. 陆万香,李名扬,裴炎等. 玉米几丁质酶基因导入甘蓝型油菜的研究[J]. 西南农业大学学报,2001,23(2):130-133
47. 罗晓芳等.金丝小枣组织培养快速繁殖的研究[J].北京林业大学学报.1996,18(2):9-15.
48. 倪德祥,冯文煦,张丕方等. 光质对一品红叶离体培养中形态发生及生化变化的影响[J]. 广西植物,1987,7(4):339-345
49. 潘磊庆,屠康,贾明敏等.臭氧水处理对芹菜保鲜效果的研究[J]. 安徽农业大学学报,2004,31(3):348-352
50. 潘瑞炽,董愚得.植物生理学[M].北京:高等教育出版社,1995
51. 潘瑞炽.植物组织培养[M].广东:广东高等教育出版社,2001.
52. 彭方仁.无性繁殖的进展及其在无性系林业中应用前景[J].世界林业研究,1990,3(4):74-80.
53. 卜学贤,陈维纶. 试管植物的玻璃化现象. 植物生理学通讯,1987,23(5):13-9
54. 祁春芳,郑智礼. 白杨派杨树组织培养技术研究. 山西林业科技,2000,(4):21-23
55. 齐燕红. 毛白杨快繁及玻璃化研究[D]. 北京:北京林业大学图书馆,1993
56. 沈效东,张新宁,袁世杰等. 生长调节剂对毛白杨叶愈伤组织诱导和植株再生的影响[J]. 宁夏农林科技,1996,(6):18-21
57. 施和平,李玲,潘瑞炽. 乙酰丁香酮对发根农杆菌遗传转化黄瓜的影[J]. 云南植物研究, 1997,19(4):445-448
58. 孙敬三,桂耀林 主编.植物细胞工程实验技术[M]. 北京:科学出版社,1995
59. 孙雪新,陈红.胡杨组织培养.植物学通报[J],1992,9(增刊):49-50.
60. 孙雪新,康向阳,李毅等. 胡杨的研究现状及发展建议[J]. 世界林业研究,1993,(4): 48-52
61. 孙雪新,康向阳.胡杨无性繁殖研究[J],甘肃林业科技,1993(1):27-30.
62. 谭文澄,戴策刚.观赏植物组织培养技术[M].中国林业出版社,1991.
63. 汪志军,李康,处格鲁克·艾克等.胡杨的组织培养[J].新疆农业科学,1997(4),185-186.
64. 王关林,方宏筠 主编.植物基因工程原理与技术[M].北京:科学出版社,1998.
65. 王敬驹,匡柏健,曾慧.提高甘蔗组织培养效率的研究[J].植物学通报,1983,1(2):17
66. 王明庥.林木遗传育种学[M].北京:中国林业出版社,2001.4.
67. 王善平,许农,许智宏.利用 PEG 法对 GUS 基因在几种杨树原生质体中瞬间表达的研究[J].试验生物学报,1991,24:71-73.
68. 王世绩. 全球胡杨林的现状及保护和恢复对策[J]. 世界林业研究,1996,(6):37-44
69. 王影,黄敏仁,陈道明等. 杨树细胞悬浮培养及体细胞胚胎发生的研究[J]. 南京林业大学学报,1991,15(3):31-36
70. 王勇.抗生素对桑树外植体生长与分化的影响[J].蚕业科学,1996,22(2):72-76.
71. 魏庆主编.胡杨[M].北京.中国林业出版社,1998. 104-141.
72. 吴颂如.酶联免疫法测定植物内源激素[J].植物生理学通讯,1988,(5):53-56
73. 吴延军.桃组织培养及遗传转化的研究[D].杭州:浙江大学图书馆,2004
74. 夏阳,梁慧敏,孙仲序等. 四倍体刺槐转甜菜碱醛脱氢酶基因的研究[J]. 山东林业科技,2003,(3):1-3
75. 徐妙珍等,林木组织培养的障碍因素及对策(一)[J],林业科技.1991,16(4):5-6
76. 徐妙珍,张雅君,李伟等. 林木组织培养的障碍因素及其对策. 林业科技,1992,17(3): 10-12
77. 徐纬英.杨树选种学[M].科学出版社,1960
78. 续九如,黄智慧 编著.林业试验设计[M].北京:林业出版社,1995
79. 杨秀荣,陈永文,方平. 乙酰丁香酮对根癌农杆菌介导的甘薯遗传转化的影响[J]. 西南师范大学学报(自然科学版),2002,27(5):52-56
80. 尹淑萍,草莓栽培品种离体再生及遗传转化的研究[D].中国农业大学,2004
81. 于建云. 散射光、变温处理对香石竹玻璃苗的影响研究[J].华东森林经理,2002,16(4):27-28
82. 陆万香,李名扬,裴炎等. 玉米几丁质酶基因导入甘蓝型油菜的研究[J]. 西南农业大学学报 2001,23(2):130-133
83. 詹亚光.杨树花药培养筛选耐盐变异体的研究[J].植物研究,1994,14(1):98-103.
84. 张克,郁东宁.胡杨茎尖组织培养的初步研究[J].宁夏农林科技,1989(5):25-26.
85. 张丕方,倪德祥,包慈华编著. 木本植物组织培养与繁殖上的应用. 上海:上海教育出版社,1985
86. 张平冬,康向阳,高鹏,韩锦. 胡杨离体培养分化增殖途径的比较研究[J].北京林业大学学报,2003,25(6):50-54
87. 张平冬. 胡杨无性繁殖与优树选择研究[D].北京林业大学,2004.
88. 张卫芳,高疆生,段黄金等. 胡杨离体快繁技术研究[J]. 新疆农业科学,2001,38(6): 320-322
89. 章镇,孙爱君,房经贵,盛炳成.农杆菌介导 rolC 基因转化烟草植株的研究[J].南京农业大学学报,2001,24(1):25-29.
90. 赵华燕,卢善发,晁瑞堂.杨树的组织培养及其基因工程研究[J].广西植物,2001,18(2):169-176.
91. 郑丽.根癌农杆菌介导 SAG12-ipt 和 3DN-iaaL 基因转化切花菊及抗早衰研究[D].西南农业大学,2003
92. 周立刚,杨崇仁,王君健.露水草毛状根的诱导和培养[J].云南植物研究,1996,18(3): 336-340.
93. 周林元,桂耀林. 植物学集刊.离体培养下胡杨的器官发生和小植株形成[C].中国科学院植物研究所,科学 1983, 1(1)
94. 朱大保.国外杨树组培微繁技术的进展[J].北京林业大学学报,1990,12(1):84-91.
95. 朱广廉,植物组培中的外值体灭菌[J],生理学通讯.1996,32(6):444-449.
96. 朱建华,彭士勇 编著. 植物组织培养实用技术[M]. 北京:中国计量出版社,2001
97. 诸葛强,王婕琛,陈英等.新疆杨高效遗传转化系统的建立[J].植物资源与环境学报,2003,12(4):6-10.
98. 张桂花 王洪梅 王连祥牡丹组织培养技术研究[J]. 山东农业科学, 2001,(5):16-18
99. Arnold Sara Von, Induction and development of adventitious bud Primordia On embryos, buds and needles of Norway spruce (Picea abies (L.) Karst.) grown In Vitro, Institute of Physiologicl Botany University of Uppsala, Sweden, 1979,5(11):1-32
100.Barbare M.Reed,Jessica Mentzer,Piyarak Tamprasert&Xiao Lingyu,intenal bacterial contam inatio of micropropogated hazelnut: identification and antibiotic treatment [J], Plant cell, Tissue and organ culture.1998(52):67-70.
101.Berbee FM,et al. Induction of callus and tree from stem tip cultures of a hybrid poplar [J]. In Vitro,1972,7,269.
102.Brand R. The formation of adventitious organs Ⅱ.The origin of buds formed on young adventitious roots of Populous nigra L. ‘Italica’ [J]. Acta. Bot. Neerl.,1973,22(4):399-406.
103.Cheong EunJu; Moon Heung KYu. Effect of light, cytokinins and explant’s position on adventitious bud induction from the leaf segments of P. euphratica [J] (FRI) Journal of Forest Science (Seoul) (1999).No.62,92-98
104.Chupeau M.C , Pautot V , Chupeau Y. Recovery of transgenic tree safter electroporation of poplar protoplasts [J]. Transgenic Res.,1994,3:13-19.
105.Costantino P , Capone I , Cardarelli M , De Poalis A , Mauro ML , Trovato M. Bacterial plant oncogenes:the rol genes saga[J]. Genetica,1994,94:203-211.
106.D’halluin K, Bonne E, Bossut M et al. Transgenic maize plants by tissue electroplation. Plant Cell[J],1992,4:1495-1505
107.Debergh P , Aitken-christie J , Cohend , et al. Reconsideration of the term ‘vitrification’ as used in micropropagation [J]. Plant Cell Tiss. Org. Cult.,1992,30:135-140.
108.Estruch JJ,Chriqui D,Grossmann K,Schell J,Schmulling T.The plant oncogene rolC is responsible for the release of cytokinins from glucoside conjugates[J]. EMBO J,1991b,10(10):2889-2895.
109.Estruch JJ,Parets-Soler A,Schmulling T,Spena A.Cytosolic localization in transgenic plants of the rolC peptide from Agrobacterium rhizogenes[J]. Plant Mol. Biol.,1991c,17:547-550.
110.Gelvin SB. Crown gall disease and hairy root disease : A sledgehammer and a tackhammer [J]. Plant Physiol,1990,92:281-285.
111.Gu, R . S . , Jiang, X . N . , Guo,ZH . CH . Organogenesis and plantlet regeneration in vitro of Populus euphratica [J] . Acta Botanica Sinica. Jan.1999,41(1):29-33
112.Huang F H,Li X Y. Effects of concentration of acetosyringone and Agrobacterium tumefaciens on gus gene transformation efficiency of Populus[J]. In vitro Cell Dev. Biol.,1994,30A:67.
113.J . Washer, Differentation in Pinus radiata Callus Culture: The effect of nutrients [J]. New Zealand Journal of Forest Science, 1977,7(3):321-328
114.Jouanin L,Guerche P,Pamboukdjian N,Tourneur C,Casse-Delbart F,Tourneur J. Structure of T-DNA in plants regenerated from roots transformed by Agrobacterium rhizogenes strain A4[J].Mol Gen Genet,1987,206:387-392.
115.Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature,1970,227:680-685.
116.Leple JC , Miranda B , Michel MF et al. Transgenic poplars : expression of chimerical genes using four different constructs[J]. Plant Cell Reports,1992, 11:137-141.
117.Lledo M D, Crespo M B, Amomasco J B . The role of cytokinins and ethylene inhibitors on lenticel hypertrophy generation and ethylene production in vitro cultures of Populus euphratica Oliver [J]. Israel of Plant Science, 1995,43: 339-345
118.Ludlow M.M,Muchow RC.A critical evaluation of traits for improving crop yield in water-limited environments[J]. Advanced in Agronomy,1990,43:107-153.
119.Parsons TJ , Sinkar VP , Stettler RF et al.Transformation of poplar by Agrobacterium tumefaciens[J]. Bio/technology,1986,4(6):533-536.
120.Petit A,David C,Dahl GA,Ellis JG,Guyon P,Casse-Delbart F,T empe J. Further extension of the opine concept:plasmids in Agrobacterium rhizogenes cooperate for opine degradation[J]. Mol. Gen. Genet,1983,190:204-214.
121.Voskresenskaya N P, Drozdova T s, Krendeleva T E Efect of light quality on the organization of photosynthetic eletron transport chain of pea seedlings. Plant Physicl[J]. 1977,59:15
122.Renate Muller,Arne S.Ansderson,Mangrethe Serek. Ditterences in display life of minatare potted roses [J]. Scientia Horticulturne,1998,76:59-71.
123.Routn GR,Samantaray S,Mottley J,Das P. Biotechnology of the Rose:A review of recent progress[J]. Scientia Horticulturae,1990(81):201-228.
124.Salehi H , Khosh-khui MA. Simple procedure for disinfection of ‘baby masquerade’ miniature rose explants [J]. Scientia Horticulturne,1997,68: 145-148.
125.Schmulling T,Fladung M,Grossman K,Schell J. Hormonal content and sensitivity of transgenic tobacco and potato plants expressing single rol genes of Agrobacterium rhizogenes T-DNA [J]. Plant J.,1993,3:371-382.
126.Schmulling T,Schell J,Spena A. Promoters of the rolA, B and C genes of Agrobacterium rhizogenes are differentially regulated in transgenic plant[J]. Plant Cell,1989,1:665-670.
127.Sinnott E W. Plant morphogenesis [M].New York:McGraw-Hill,1960.
128.Slightom JL,Durand-Tardif M,Jouanin L,Tepfer D. Nucleotide sequence analysis of TL-DNA of Agrobacterium rhizogenes agropine type plasmid [J]. J. Biol. Chem.,1986,261:108-121.
129.Souq F.,coutos-thevenot P.,Yean H.,et al. Genetic transformation of roses,2 examples:one on morphogensis,the other on anther cyan biogynthe-tic pathway [J]. Acta. Hort.,1996 (424):381-388.
130.Turk S CH J, Melchers L S Den Dulk-Ras H et al. Environmental conditions differentially affect vir gene induction in different Agrobacterium strains. Role of the vir A sensor protein. Plant Mol Biol[J], 1991, 16:1051-1059
131.Tzfira T,Vinnocur B,Altman A,et al. rol-transgenic Poplus tremula:root development,root-bore bud regeneration and in vitro propagation efficiency[J]. Trees,1998,12:464-471.
132.Van der Salm TPM,Hanisch ten Cate CH, Dons HJM. Prospects for application ofrol genes for crop improvenment. Plant Mol Biol Rep. 1996,14(3):207-228
133.Van der Salm TPM,Bouwer R,Van Dijk A J,Keizer LCP,Hanisch ten Cate ChH,Van der Plas LHW,Dons JJM. Stimulation of scion bud release by rol gene transformed rootstocks of Rosa hybrida L [J]. J. Exp. Bot.,1998,49 (322):847-852.
134.Vardi A, Bleichmans, Aviv D. Genetic transformation of transgenic plant [J]. Plant Science,1990,69:199-206
135.Voskresenskaya N P, Drozdova T s, Krendeleva T E Efect of light quality on the organization of photosynthetic eletron transport chain of pea seedlings [J]. Plant Physicl. 1977,59:15
136.Watanabe M, Pawlicki N, Holefors A, et al. Genetic transformation of apple rootstock M26 with rolB gene and its influence on rooting. J Plant Physiol, 1998,53:371-380
137.Watanabe S, Kihuna K, Ide Y, Sasaki S. Establishment of a tissue culture system of Populus euphratica oliv [J]. Bulltin of the Tokyo University Forests, 1999,102:87-92
138.White FF,Taylor BH,Huffman GA,Gordon MP,Nester EW. Molecular and genetic analysis of the transformed DNA regions of root-inducing plasmid of Agrobacterium rhizogenes [J]. J Bacteriol. 1985,164:33-34.
139.Winton L. Tissue culture propagation of European aspen [J]. Forest Sci.,1971, 17 (3);348-350.
140.Ziv M , Ariel T. Vitrification in relation to stomatal deformation and malfunction in carnation leaves in vitro [M].Growth and Development of Micropropagated Plants. Kluwer Academic Press,Dordrecht,1994:143-154.
141.Bleecker AB,Schutte JL, Kende H. Antomical analysis of growth and development patterns in the internode of deeper water rice [J]. Planta,1986,169:490-497.
142.Cho HT , Kende H. Expression of expansin genes is correlated with growth in deepwater rice[J]. Plant cell,1997,9:1661-1667.
143.Cosgrove DJ. Loosening of plant cell wall by expansinse [J]. Nature,2000, 407:321-324.
144.Davis PJ. Plant hormones:physiology,biochemistry and molecular biology[M].. Dordrecht. The Netherlands:Kluwer academic publishers.1995,13-38.
145.Duckett CM , Lloyd CW. Gibberellic acid-induced microtubule reorientation in dwarf peas is accompanied by rapid modification of an α-tubulin isotype [J]. Plant J.,1994,5:363-372.
146.Fabian T,Lorbiecke R,Umeda M,Sauter M. The cell cycle genes cycA1-1 andcdc2Os-3 are coordinately regulated by gibberellin in planta [J]. Planta ,2000,211:376-383.
147.Giddings TH , Staehelin LA.Microtubule-mediated control of microfibril deposition:a re-examination of the hypothesis[M]. In The Cytoskeletal Basis of Plant Growth and Form,Academic Press,London,1991,85-99.
148.Hea PM , Yoshihito S , Makiko C , Paul KJ , Isomaro Y.CsAGP1 , a gibberellin-responsive gene from cucumber hypocotyls,edcodes a classical arabinogalactan protein and is involved in stem elongation[J]. Plant Physiol,2003,131(3):1450-1459.
149.Kurioka Y , Suzuki Y , Kamada H , Harada H. Promotion of flowering and morphological alterations in Atropa belladonna transformed with a CaMV 35S-rolC chimeric gene of the Ri plasmid [J]. Plant Cell Rep,1992,12:1-6.
150.Lorbiecke R , Sauter M. Induction of cell grown and cell division in the intercalary meristem of submerged deepwater rice[J].Planta,1988,204:140-145.
151.Mizuno R.Inhibition of gibberellin-induced elongation , reorientation of cortical microtubules and change of isoform of tubulin in epicotyl segments of Azuki bean by protein kinase inhibitors[J]. Plant Cell Physiol,1994,35:1149-1157.
152.Olszewski N,Sun TP,Guble F. American Society of plant biologists gibberellin signaling : biosynthesis , catabolism , and response pathways[J]. Plant Cell , 202,S61-S80.
153.Oono Y,Suzuki T,Toki S,Uchimiya H. Effects of the over-expression of the rolC gene on leaf development in transgenic periclinal chimeric plants [J]. Plant Cell Physiol,1993,34:745-752.
154.Shibaoka H. Plant hormone-induced changes in the orientation of cortical microtubules [J]. Plant Mol. Biol.,1994,45:527-544.
155.Uozu S , Tanaka-Ueguchi M , Kitano H , Hattori K , M Characterization of XET-ralated genes of rice[J]. Plant physiol,2000,122:853-859.
156.Van der Knaap,Jeong Hoe Kim,Hans Kende. A novel gibberellin-induced gene from rice and its potential regulatory role in stem growth [J]. Plant Physiol ,2000,122:695-704.
157.Yang GX,Jan A,Shen SH,Yazaki J,Ishikaw M a,Shimatani Z,Kishimoto N,Kikuchi S,Matsumoto H,Komatsu S. Microarray analysis of brassinosteroida and gibberellin-regulated gene expression in rice seedlings [J]. Mol. Genet Genomics,2004,271 (4):468-478.
158.Yaxley JR,Ross JJ,Sherriff LJ,Reid JB. Gibberellin biosynthesis mutationsand root development in pea [J]. Plant Physiol,2001,125:622-633.
159.Yi L,Hans K. Expression of alpha-expansin and expansin-like genes in deepwater rice [J]. Plant Physiology,2002,130(3):1396-1405.