摘要
本研究采用水提醇沉法从中药黄芪中得到一种粗提物,硫酸-苯酚法测得其中多糖纯度为36.12%。另外对黄芪多糖及其水解后产物进行紫外光谱分析,结果表明黄芪多糖紫外光谱没有出现核酸和蛋白质、氨基酸类物质的特征吸收,水解后产物在300nm 处与葡萄糖和半乳糖有相同吸收峰,且发现水解液在230nm 处有最大吸收。
通过体外抑菌试验研究了黄芪多糖对2株益生菌和3株致病菌生长的影响以及2株益生菌对致病菌株的抑制作用。结果50μl、100μl 和200μl 0.1%的黄芪多糖水溶液对2 株益生菌(乳酸菌和芽孢杆菌)、3 株致病菌(葡萄球菌、大肠杆菌和沙门氏菌)均有抑制作用(P<0.05);一定浓度的乳酸菌和芽孢杆菌对3 株致病菌也有显著抑制作用(P<0.05)。
本研究将240 只商品代海兰蛋雏鸡按体重随机分为4 组,第1 组为空白对照组,2、3、4 组为试验组,在基础日粮基础上分别添加220mg/kg 黄芪多糖、4×107cfu/g 益生菌剂以及220mg/kg黄芪多糖+4×107cfu/g益生菌剂,观察黄芪多糖和益生菌对雏鸡生长和免疫的协同作用。结果显示黄芪多糖、益生菌和合生元均能显著提高雏鸡周增重(P<0.05),减少饲料消耗(P<0.05),降低料肉比(P<0.05),但未发现合生元组雏鸡生产性能与黄芪多糖和益生菌组有明显差异(P>0.05)。试验期内雏鸡血清总蛋白含量试验组均较对照组升高(P>0.05),血清葡萄糖含量试验组均较对照组降低(P>0.05)。合生元显著增加乳酸菌和双歧杆菌的数量(P<0.05),减少大肠杆菌的数量(P<0.05),且较单一添加差异显著(P<0.05)。
添加合生元对雏鸡免疫功能有明显增强作用。合生元组整个试验期法氏囊和脾脏指数均显著高于空白组、黄芪多糖组和益生菌组(P<0.05);3 个试验组雏鸡血清新城疫抗体效价均较对照组升高了19.30%、22.81%、28.07%和32.56%、27.91%、46.51%,差异显著(P<0.05),且以合生元对新城疫抗体效价的影响最显著(P>0.05);合生元组雏鸡ANAE 阳性细胞率2 个日龄均较对照组和黄芪多糖组差异显著(P<0.05),与益生菌组差异不显著(P>0.05);试验组雏鸡红细胞C3b 受体花环促进率均较对照组显著提高(P<0.05),抑制率均降低,但只有42 日龄合生元组差异显著(P<0.05);3 个试验组之间红细胞花环促进率除42 日龄合生元组显著高于黄芪多糖组(P<0.05)外,其余均差异不
A crude production extraction of Astragalus membranaceus , Astragalus polysaccharide (APS) ,was gained through hot water extracting and ethanol depositing. The polysaccharide content of crude APS was 36.12% determined by vitriol-phenol method. The ultravioley spectrum revealed that APS did not contain protein and nucleic acid. the hydrolyzed production of APS had the same most absorbance with glucose and galactose at 300nm. And the hydrolyzed production had also the most absorbance at 230nm.
Antimicrobial activity was studied of APS to Lactobacillus (L.lactis), Bacillus and E.coli, S.typhimurium, Sta.aureus and the activity of Lactobacillus and Bacillus to E.coli, S.typhimurium, Sta.aureus. The results showed that 50μl,100μl and 200μl 0.1% water solution of APS could significantly inhibit the proliferation of all strains(P<0.05). And some contents of Lactobacillus and Bacillus significantly inhibited E.coli, S.typhimurium and Sta.aureus.
Cooperation effects of APS and profitable microbe (PM) on growth performance and immune function of chicks were studied. 240 Hy-Line chicks were randomly divided into 4 groups. The basic diet of the 4 groups was the same and treatments were as follows: control (no APS and no PM), 220mg/kg APS, 4×107cfu/g PM and 220mg/kg APS+4×107cfu/g PM. The results showed All of APS, PM and the compounds could significantly increase Average Growth of Each Week (P<0.05), decrease forage consumption (P< 0.05), lower feed/growth (the decrease was respectively 13.96%, 15.26% and 12.34%, P<0.05). But it showed that there was no significant difference of growth performance between PM+APS group and APS group or PM group (P>0.05). Compared to the control group, serum total protein of the trial groups were higher (P<0.05), but serum glucose was lower (P<0.05). The population of L.lactis and Bifido. Of PM+APS group were significantly higher (P<0.05) E.coli was significantly lower(P<0.05), and the action excelled any single one(P<0.05).
Immunopotentiation of the compounds of APS and PM were found in the research. The index of Bursa of Fabricius and Spleen of APS+PM group were both significantly higher than the others (P<0.05). Serum antibody titer to Newcastle disease vaccine of trial groups were remarkably higher than control group (P<0.05), and the increasing extent were respectively 19.30%、22.81%、28.07% and 32.56%、27.91%、46.51%. And the effect of APS+PM group is the best remarkable(P>0.05). ANAE positive cells of APS+PM group was significantly higher (P<0.05) than that of control group and APS group in 21 day and 42 day(P<0.05), but not significantly
than that of PM group(P>0.05). RFER of three trial groups were all significantly higher than the control group (P<0.05). RFIR of three trial groups were all decreasing(P>0.05), but Only APS+PM group was significant in 42 days(p<0.05). Among three trial groups, RFER were not significantly increasing(P>0.05) except the APS+PM group in 42 days to the APS group(p<0.05). RFIR of APS group and PM group were both more than the APS+PM group, but only while in 42 days it was significantly(P<0.05). It was studied on the tissue structure of chick. Compared to the control group, lymph follicle of Bursa of Fabricius increased in size and the plica increased. Thymic corpuscles increased in size and nummer. Periarteriolar lymphoid increased in size and splenic corpuscle increased in nummer. Villus of Ileum and Cecum increased in high and width and was arranged tightly. Glblet cells and lymphocyte of mucosa increased in size. Wall of insteines of chick increased in thickness in 21 days and became thin with increase of the days.
引文
[1] Adler B B, Beuchat L R. Death of Salmonella, Escherichia coli 0-157, and Listeria monocytogenesin garlic butter as affected by storage temperature[J].Journal of Food Protection,2002,65(12):1976-1980.
[2] Leuschner R G K, Ielsch V. Antimicrobial effects of garlic, clove and red hot chilli on Listeria monocytogenes in broth model systems and soft cheese[J]. International Journal of Food Sciences and Nutrition, 2003, 54(2):127-133.
[3] Sasaki J,Kita T, Ishita K, et al. Antibacterial activity of garlic powder against Escherichia coli 0-157[J]. J. Nutr. Sci. Vitaminol., 1999, 45(6):785-789.
[4] 严常开,曾繁典.大蒜的主要化学成分及其药理作用研究进展[J].中国新药杂志,2004,13(8):688-691.
[5] 张为民,张淑霞,张耀相,等.抗病毒合剂药理作用研究之二——体外抑菌作用观察[J].动物医学进展,2002,23(4):66-67.
[6] 胡元亮,刘家国,陈玉库,等.中药成分对传染性法氏囊病毒感染细胞的影响[J].畜牧与兽医,2003,35(12):8-10.
[7] 孔祥峰,胡元亮,王德云,等.中药成分与新城疫病毒感作后对病毒感染活性的影响[J].南京农业大学学报,2004,27(2):90-93.
[8] 陈忠法,朱鹰飞.辣椒粉对仔猪生产性能影响的研究[J].浙江万里学院学报,2001,14(3):24-25.
[9] 胡太文,曹树萍,陈益众.辣椒粉可促进肥育猪增长[J].中国饲料,1994,(8):35.
[10] 李晓亮,李湘,张阅军,等.松针粉营养成分分析[J].饲料工业,1997,18(5):31-32.
[11] 陈宝江,王建辉,李树友,等.松针粉对雏鸡生长及免疫力影响的研究[J].饲料研究,2000,(4):28-30.
[12] 段树清,赵宏伟,胡景立.在肉种鸡饲料中添加松针粉对其育成率、生产率的试验[J].石河子科技,1998,(4):55-56.
[13] 姜锦鹏,高和坤,柳丽,等.海藻粉作蛋鸡饲料研究[J].中国饲料,2000,(3):29-30.
[14] 谷海源,刘永刚,舒子贞.海藻作饲料添加剂饲喂产蛋鸡的效果[J].饲料研究,1998,(9):1-2.
[15] 张润栋,魏建平,李晓华.红辣椒提高蛋黄色度的研究[J].饲料博览,1999,11(9):26-27.
[16] 曲湘勇,中岛隆.天然着色剂提高蛋黄色泽度的比较研究[J].中国畜牧杂志,1999,35(2):29-31.
[17] 王俊东,李淑琴,申瑞玲,等.中药对肉仔鸡脂质代谢及肉品质的影响[J].中国兽医学报,2003,23(4):377-379.
[18] 邵淑丽,徐兴军,邵会祥,等. 沙棘嫩枝叶对鸡生长性能及肉质的影响[J]. 黑龙江畜牧兽医,2002,(5):12-13.
[19] 曹燕,刘振国.刺五加提取物可提高母牛乳的生产力[J].饲料广角,1995,(5):31.
[20] 王瑞云,徐长德.刺五加饲喂蛋种鸡效果研究[J].畜禽业,2000,(7):50-51.
[21] 史万玉,包永占,褚素敏,等.不同中药复方抗鸡柔嫩艾美耳球虫的效果观察[J].黑龙江畜牧兽医,2003,(7):28-29.
[22] 史万玉,李呈敏,包永占,等. 抗鸡柔嫩艾美耳球虫中药复方的筛选(初报) [J].中兽医医药杂志,2003,(2):15-17.
[23] 史万玉,李呈敏,包永占,等. 抗鸡柔嫩艾美耳球虫中药复方的筛选(二报) [J].中兽医医药杂 志,2004,(2):8-9.
[24] 青蒿素结构研究协作组.一种新型的倍半萜内酯——青蒿素[J].科学通报,1977,(22):142.
[25] 纪丽莲,张强华.八种菊科中草药抗霉菌及饲料霉变的研究[J].生命科学研究,2003,7(4):350-354.
[26] 袁萍,王国亮,龚复俊,等.3 种植物精油对霉菌的抑制作用[J].武汉植物学研究,2001,19(6):521-523.
[27] 余伯良,罗惠波,周健,等. 山苍子油抗霉菌及抑制黄曲霉产毒的有效成分研究[J].四川轻化工学院学报,2002,15(1):32-36.
[28] 周维纯,王金秋,宋金表,等.松针提取物饲料添加剂生产性试验[J].林产化工通讯,1997,(3):6-9.
[29] Kitagawa I, Wang H, Saito M, et al. Chemical constituents of Astragali radix, the root of Astragalus membranaceus bunge(2). Astragalosides I, II, IV, acetylastragaloside I and isoastragalosides I and II [J]. Chem Pharm Bull, 1983,31(2):698.
[30] 曹正中,俞家华,张勤.膜荚黄芪化学成分的研究[J].中草药,1985,16(6):278-279.
[31] 王志学,马启凤,何群,等.黄芪化学成分的研究[J].中草药,1983,14(3):97-99.
[32] 吕归宝,吕曙华,张国庆,等.黄芪中黄酮类化合物的分离鉴定[J].中草药,1984,15(10):20-22.
[33] 宋纯清,郑志仁,刘涤,等.膜荚黄芪中两个新的抗菌异黄烷化合物[J].植物学报,1997,39(5):486-488.
[34] 黄乔书,吕归宝,李雅臣,等.黄芪多糖的研究[J].药学学报.1982,17(3):200-206.
[35] 方圣鼎,陈燕,徐小异,等.中药黄芪有效成分的研究多糖体的分离、性质及其生理活性[J].有机化学,1982,(1):26.
[36] Kajimura K, et al.黄芪的药性:对体外抗体生成作用的影响[J].Natural Medicines, 1997, 51(1):45.
[37] 周克年,周明.黄芪苦参山饲喂雏鸡的效果[J].中国畜牧杂志.1998,34(3):33-34.
[38] 赵建华,陈杰.黄芪粉蛋鸡饲料添加剂效果观察[J].中兽医学杂志.1996,(2):8-9.
[39] 王玉山.黄芪对肉仔鸡增重的影响[J].中国家禽,1995,(5):14-15.
[40] 祝国强,杜云良,王斌,等.复方中药对肉鸡生产性能和生化指标的影响[J].中国家禽,2004,26(15):23-24.
[41] 王福传,韩一超,张玉换,等.兽用中草药高效免疫增强剂研究初报[J].山西农业科学.2001,29(2):74-80.
[42] 吴长德,吕戴春,方永辉.复方女贞子-黄芪制剂对雏鸡的免疫增强作用[J].黑龙江畜牧兽医,2003,(1):30-31.
[43] 催保安,张素梅,杨名凡,等.黄芪与左旋咪唑对雏鸡免疫功能的比较[J].兽药与饲料添加剂,2001,(6):1-2.
[44] 陈玉库,胡元亮,张宝康,等.单味主药及其复方药治疗鸡传染性法氏囊病的疗效比较[J].江苏农业学报,2003,19(1):41-43.
[45] 赵银丽,李国喜,李慧勤.黄芪对固始鸡免疫性能的影响[J].河南农业科学,2004,(11):90-91.
[46] 高微微,曹丽,李展,等. 黄芪成分对鸡外周血淋巴细胞增殖反应的影响[J].中国兽医杂志, 2001,35(6):16-18.
[47] 阴健,郭力弓.中药现代研究与临床应用[M].北京:学苑出版社,1993, 592.
[48] Chen H L, Li D F, Chang B Y, et al. Effects of Chinese herbal polysaccharides on the immunity and growth performance of young broilers[J]. Poult Sci, 2003,82(3): 364-370.
[49] 聂小华,史宝军,敖宗华,等.黄芪活性成分的提取及其对淋巴细胞增殖的影响[J].无锡轻工大学学报,2003,22(4):49-51.
[50] 孔祥峰,胡元亮,李祥瑞,等.9 种中药成分对新城疫系疫苗免疫雏鸡血清中血凝抑制抗体水平的影响[J].畜牧兽医学报,2004,35(4),468-470.
[51] 储岳峰,李祥瑞,胡元亮.9 种中药成分对小鼠免疫细胞活性的影响[J].南京农业大学学报,2004, 27(1):97-100.
[52] 储岳峰,颜新敏,胡元亮,等.几种中药成分的免疫增强活性及其作用效果[J].中国兽医科技 2005,35(1):67-70.
[53] 王德云,胡元亮,孔祥峰,等. 中药成分对雏鸡外周血T 淋巴细胞转化的影响[J].中国兽医学报,2004,24(6):578-580.
[54] 杨凤华,康成,李淑华,等. 黄芪水溶性黄酮类对小鼠细胞免疫功能的影响[J].时珍国医国药,2002,(12):718-719.
[55] 焦艳,闻杰,张德山.膜荚黄芪茎叶总黄酮对小鼠免疫功能的影响[J].中医药信息,1997,(5):44.
[56] 夏英.黄芪多糖(APS)免疫作用的实验研究[J].上海中医药杂志,1994(11):42-43.
[57] 孙月平,王金宝,张乐萃,等.黄芪多糖对免疫抑制鸡某些免疫指标影响的研究[J].莱阳农学院学报,1999,16(1):61-63.
[58] 樊福好,谢明权,林辉环.3 种免疫增强剂对鸡的免疫促进效果试验[J].中国兽医杂志,2001,37(2):12-13.
[59] 唐雪明,胡元亮,张宝康,等.黄芪多糖对雏鸡外周血T 淋巴细胞转化功能的影响[J].中国兽医学报,1998,18(3):269-271.
[60] 胡庭俊,梁纪兰,程富胜,等.黄芪多糖对鸡脾脏淋巴细胞内游离钙离子浓度的影响[J]. 中兽医医药杂志,2001,(5):4-6.
[61] 陈洪亮.植物多糖的制备及对肉仔鸡免疫功能影响的研究[D].北京:中国农业科学院,2002.
[62] 孟宪荣,李庆章,曲琪环,等.黄芪多糖和香菇多糖对马立克氏病强毒感染雏鸡巨噬细胞活性和白细胞介素-1 体外诱生活性的影响[J].中国兽医杂志,2002,38(7):33-34.
[63] 高学军,李庆章.黄芪多糖和香菇多糖对雏鸡红细胞免疫功能的影响[J].东北农业大学学报,2000,31(1):69-71.
[64] 高学军,李庆章.黄芪多糖和香菇多糖对vMDV 人工感染雏鸡红细胞免疫功能的影响[J].东北农业大学学报,2000,31(2):169-172.
[65] 李宏全,段县平,马海利,等.黄芪多糖对鸡新城疫和传染性腔上囊病疫苗免疫力的影响[J].中国兽医科技,2001,31(9):12-14.
[66] 张述斌,薛掌林,刘瑞生,等.黄芪多糖、淫羊藿多糖对鸡新城疫疫苗免疫增强作用的研究[J].甘肃畜牧兽医,2004,(4):24-25.
[67] 耿长山,邢善田,周金黄.黄芪多糖对去T 细胞小鼠促进抗体产生机理的探讨[J].上海免疫学杂志,1995,5(2):69.
[68] 赵克胜,丁丽轩,孔海燕,等 黄芪多糖增强人外周血单个细胞产生肿瘤坏死因子的研究[J].中国中西医结合杂志,1993,(13):263.
[69] 刘永杰,李庆章,郝艳红.香菇多糖和黄芪多糖对马立克氏病强毒感染雏鸡白细胞介素2 活性和淋巴细胞增殖反应的影响[J].中国兽医杂志,1999,25(7):3-5.
[70] 孟宪荣,李庆章,曲琪环,等.黄芪多糖和香菇多糖对马立克氏病强毒感染雏鸡巨噬细胞活性和 白细胞介素-1 体外诱生活性的影响[J].中国兽医杂志,2002,38(7):33-34.
[71] Nathan S, Halina L. Carbohyrates in cell recognition[J]. Sci Ameri, 1993,(1):74-81.
[72] 田碧文.阿胶、五味子、刺五加、枸杞对双歧杆菌生长的影响[J].中国徽生态学杂志,1996,8(2):11-13.
[73] 温朗聪,马晓琼,董永军,等.微生物添加剂与抗生素预混料对肉鸡的饲养效果比较[J].中国微生态学杂志,2000,12(5):268-269.
[74] 胥清富,王金洛,李猛,等. K94 复合活菌剂对蛋鸡产蛋性能、饲料消化率和盲肠菌群的影响[J].畜牧与兽医,1999,31(2): 12-14.
[75] 张春杨,牛钟相,常维山,等.益生菌剂对肉用仔鸡的营养、免疫促进作用[J].中国预防兽医学报,2002,24(1):51-54.
[76] 叶成远.寡糖饲料添加剂[J].畜禽业,1999,(10):12-13.
[77] Omar A, Oyarzabal. In vitro fructooligosacharide utilization and inhibition of salmonellas Spp [J]. Poultry of Sci, 1995, 74(9):1.
[78] 吴天星,戴贤君,吴林友. 果寡糖对肉鸡生产性能的影响[J].浙江农业学报,1999,11(2):85-87.
[79] 王小平,吴凡,朱学福.EM 添加剂在蛋鸡日粮中应用效果[J].中国饲料,1997,(10): 22-23.
[80] 刘卫东,王章存. 微生态制剂饲喂产蛋鸡的效果[J].饲料工业,1999,20(9): 16-17.
[81] 陈德华,吉林,朱建. 光合细菌添加剂饲养蛋鸡试验[J]. 饲料工业,1995,15(5):11-12.
[82] 金加明,杨虎,吴宝霞,等. 酵母培养物和寡糖对产蛋高峰期蛋鸡生产性能的影响[J]. 饲料研究,2005,(3):44-45.
[83] 刘克琳,何明清,余成瑶,等.鸡微生物饲料添加剂对肉鸡免疫功能影响的研究[J].四川农业大学学报,1994,12(增刊):606-612.
[84] 张辉华,曹永长,毕英佐,等.热灭活乳杆菌制剂对竹丝肉鸡免疫性能的影响[J].畜牧与兽医,2003,35(11):14-15.
[85] 曾正清,谢幼梅,穆奎.酵母饲料对肉仔鸡肠道菌群及免疫机能的影响[J].山东畜牧兽医,2001,(5):11-13.
[86] 王秀武,杜昱光,白雪芳,等. 壳寡糖对肉仔鸡肠道主要菌群、小肠微绒毛密度、免疫功能及生产性能的影响[J].动物营养学报,2003,15(4):32-35.
[87] 马得莹,单安山,李群道,等. 中草药对蛋雏鸡生长性能和免疫功能的影响[J].中国兽医学报,2005,25(1):67-70.
[88] 邵良平,周伦江,李国平,等.甘露寡糖和粪链球菌对鸡细胞免疫和肠道微生态的调节作用[J].中国兽医学报,2000,(1):58-61.
[89] 周霞,王鹏雁,蒋建军,等.乳杆菌Ⅰ及乳杆菌Ⅱ微生态制剂对雏鸡腹泻的防治及增重试验[J].甘肃畜牧兽医,2000,(4): 8-9.
[90] 聂实践,林伯荃,朱桂茹,等.益菌多(S586)饲用微生态制剂的防病作用试验[J]. 四川农业大学学报,1999,(5):27-28.
[91] 徐春厚,马春全,绍红.乳链球活菌制剂预防肉鸡腹泻及促进增重试验[J].养禽与禽病防治,2000,(6):20-21.
[92] 毕英佐,曹永长,胡文锋.乳酸菌LB 株的特性及其在家禽生产中的应用[A].中国畜牧兽医学会动物微生态学分会.动物微生态研究进展[C].北京:中国农业大学出版社,2000,205-212.
[93] 胥清富,储文兵,王金洛. 复合活菌剂对肉用仔鸡生产性能消化和盲肠菌群的影响[J].中国畜牧杂志,1999,35(5):12-14.
[94] Kobayashi S, Eida T. Effect of fructooligosaccharides on milk-yield and milk-components of dairy cows[J]. J. Anim. Sci., 1990, (3):21-25.
[95] Sisak F. Stimulation of phagocytosis as assessed by luminal enhanced chemilumine scence and restonse to salmonella challenge of poultry feddiets containing mannan oligosaccharides[C]. In the 10th annual symposiumon Biotechnology in the Feed Industry, All tech Inc,1994:75-84.
[96] Oyofo B A, DeLoach J R, Corrier D E, et al. Effect of carbohydrates on Salmonella typhimurium colonization in broiler chickens [J]. Avian Dis, 1989, 33(3): 531-534.
[97] Oyofo B A, DeLoach J R, Corrier D E, et al. Prevention of Salmonella typhimurium colonization of broilers with D-mannose [J]. Poult Sci, 1989, 68(10): 1357-1360.
[98] 姚军虎,张长青,邓留坤,等.加藤菌对蛋鸡产蛋性能及鸡蛋品质的影响[J].饲料研究,2000,(10):10-12.
[99] 张永福.EM在蛋鸡上的应用效果试验[J].山西农业科学,1999, 27(4):81~83.
[100] 郜敏.应用EM 生物技术降低鸡蛋胆固醇的试验研究[J].环境科学研究,2000,13(3):63-64.
[101] 汪德刚,韩国府,牛瑞萍.中草药预混料对蛋鸡产蛋性能和鸡蛋品质的影响[J].郑州牧业工程高等专科学校学报,2004,24(4):241-243.
[102] 楼洪兴,林智,王友明,等.茶多酚对蛋鸡生产性能、脂类代谢及蛋品品质的影响[J].茶叶科学,2004,24(2):135—140.
[103] Noh D O, Kim S H, Gilliland S E. Incorporation of cholesterol into the cellular membrane of Lactobacillus acidophilus ATCC43121 [J]. J. Dairy. Sci., 1997, 80:3107-3113.
[104] Dambekodi P C, Gilliland S E. Incorporation of cholesterol into the cellular membrane of Bifidobacterium longum [J]. J. Dairy. Sci., 1998, 81:1818-1824.
[105] Usman, Hosono A. Biletolerance, taurocholate deconjugation, and binding of cholesterol by Lactobacillus gasseri Strains [J]. J. Dairy. Sci., 1999, 82:243-248.
[106] Tahri K, Grill J P, Schneider F. Bifidobacteria strain behavior toward cholesterol: coprecipitation with bile salts and assimilation [J]. Current Microbiology, 1996, 33:187-193.
[107] Gilliland S E, Nelson C R, Maxwell C. Assimilation of cholesterol by Lactobacillus acidophilus [J]. Appl Environ Microbiol, 1985, 49:37-381.
[108] Tahri K, Grill J P, Schneider F. Involvement of trihydroxyconjugated bile salts in cholesterol assimilation by bifidobacteria [J]. Current Microbiology,1997,34:79-84.
[109] Klaver F A M, Rvander M. The assumed assimilation of cholesterol by lactobacilli and Bifidobacterium bifidum is due to their bile salt-deconjugating activity [J]. Appl Environ Microbiol, 1993, 59:1120-1124.
[110] Stonge M P, Farnworth E R, Jones J H P. Consumption of fermented and nonfermented dairy products: effects on cholesterol concentrations and metabolism[J]. Am J Clin Nutr, 2000, 71:674-681.
[111] Pigeon R M, Cuesta E P, Gilliland S E. Binding of free bile acids by cells of yogurt starter culture bacteria[J]. J. Dairy. Sci., 2002, 85:2705-2710.
[112] Sawa T, Tomio M, Tsuneyoshi S, et al. Fructooligosaccharide Consumption Enhances Femoral Bone Volume and Mineral Concentrations in Rats [J]. J. Nutr., 2000,130:1792-1795.
[113] 赵晨曦,兰时乐,禹逸君,等.鸡粪除臭微生物菌群的筛选和应用[J].湖南农业科学,2005,(1):68-70.
[114] Fujiwara S, Seto Y. Establishment of orally-administered Lactobacillus gasseri SBT2055SR in the gastrointestinal tract of humans and its influence on intestinal microflora and metabolism [J]. Journal of Applied Microbiology, 90(3):343-352.
[115] 张建文,俞联平,刘厚福,等. 蛋鸡生产中有效微生物群技术的应用[J].甘肃农业大学学报,1997,32(4):353-356.
[116] 汪植三,廖新,苏镇松,等.有效微生物活菌菌剂净化畜舍空气效果的试验[J].华南农业大学学报,1997,18(增刊):102-105.
[117] 蒋树威,林雪影.微生态系统工程在生态畜牧业中的应用及其前景[J].家畜生态,1997,18(1):29-32.
[118] 赵芙蓉,王建平,邓云昌,等.益生菌制剂对猪生产性能及猪舍环境的影响[J].中国饲料,1998,(22):27-28.
[119] 胡学智.细菌淀粉酶生产菌株产酶能力的比较[J].工业微生物,1993,23(4): 25-33.
[120] 王子彦,康玉辉,何明清.鱼微生物饲料添加剂喂鲤鱼后对消化酶活性影响的研究[J]. 四川农业大学学报,1994,12(增刊):662-663.
[121] 陈惠,朱继喜,吕道俊.芽孢杆菌对生长育肥猪肠道菌群及酶活性的影响[J]. 四川农业大学学报,1994,12(增刊):550-553,548.
[122] Hugkes D B, Hoover D G. Viability and enzymatic activity of bifidobacteria in milk [J]. J Dairy Science, 1995, 78(2):268-276.
[123] 杨桂萍.双歧杆菌的有关酶系[J].微生物学通报,1999,(1):56-58.
[124] Desjardins M L, koy D, Toupin C. Uncoupling of growth and acids production in Bifidobacterium [J]. SSP, 1990, (6):1478-1487.
[125] 杨立志,喻春皓,何志敏.功能性低聚糖功效及生产概况[J].天津化工, 2001,(1):13-15.
[126] 陈强.动物微生态制剂的研究和应用[J].福建畜牧兽医,1997,(6):9-11.
[127] 郭芳彬.可直接饲用的微生物在畜牧业上的应用[J].饲料研究,1996,(1):13-14.
[128] 王梦亮,郭小青,梁生康.光合细菌(PSB)对鲤鱼肠道菌群及肠消化功能的影响[J].中国微生态学杂志,1999,11(3):146-147.
[129] 余成瑶,刘克琳,周毅.鸡微生物饲料添加剂饲喂肉用仔鸡扫描电镜下小肠黏膜的研究[J].四川农业大学学报,1994,12(增刊):585-587,572.
[130] 余成瑶,郑黎,周毅.肉鸡饲喂益生素后小肠黏膜上皮细胞肝细胞超微结构功能研究扫描电镜下的研究[J].四川农业大学学报,1994,14(1):61-66,56.
[131] 张力,郑中朝.饲料添加剂手册[M].北京:化学工业出版社,2000.
[132] 王长文,李永近,栾维民.持续产酸型活菌制剂对犊牛小肠绒毛形态的影响[J].吉林农业大学学报,1998,20(4):68-70.
[133] 王长文,杨连玉,栾维民等.持续产酸型活菌制剂对犊牛小肠上皮细胞胞质中细胞器的影响[J].家畜生态,2002,23(1):11-13.
[134] 胡东兴,潘康成.微生态制剂及其作用机理[J].中国饲料,2001,(3):14-17.
[135] 张春扬.益生菌剂作用机理的研究进展[J].预防兽医学进展,2000,2(4):31-34.
[136] 潘宝海,李德发,谯仕彦.益生菌、益生素及合生剂的作用机理合相互关系[J].中国饲料,2000,(15):12-14.
[137] Cummings J H. The large intestine in nutrition and disease [M]. Brussels: Institute Danone, 1997:15-42.
[138] 余成瑶,何明清,郑德成.肉用仔鸡饲喂微生物饲料添加剂(8901)细胞碱性磷酸酶(AKP)定性、定位研究[J].四川农业大学学报,1994,12(增刊):596-601.
[139] 余成瑶等.肉用仔鸡饲喂微生物饲料添加剂(8901)细胞多糖的定性、定位研究[J].四川农业大学学报,1994,12(增刊):588-595.
[140] 唐凌. 化学益生素应用现状及发展趋势[J].中国饲料,2003,(11):8-10.
[141] Jin L Z. Adhesion of Lactobacillus isolates to intestinal epithelial cell of chicken[J]. Leet Appl Microbiol, 1996, 22(3):229.
[142] Jin L Z. Effect of adherent to the intestinal epithelial cells of chicken[J]. Leet-aook-microbiol, 1996,81(2):201-206.
[143] Muralidhara K S, et al. Effect of feeding lactobacilli on the coliform and Lactobacillus flora of the intestinal tissue and feces from pigs[J]. J.Food Prot, 1997,40:288-295.
[144] 刘永杰,赵艳兵,崔玢陶,等. 给雏鸡饲喂乳杆菌对其盲肠主要正常菌群定植的影响[J]. 中国兽医科技,1999,29(5):29-30.
[145] Freter R. In vivo and in vitro antagonism of intestinal bacteria against Shigella flexneryⅡ:the inhibitory mechanism[J].Journal of infectious Diseases,1962,110:38-46.
[146] Wilson K H,Perini F. Role of competition for nutrients in suppression of Clostridium difficiel by the colonic microflora [J].Infection and Immunity,1988, 56:2610-2614.
[147] Finlay B B, Falkow S. Common themes in microbial pathogenicity revisitied [J]. Microbiology and Molecular Biology Reviews, 1997, 61:136-169.
[148] Fuller R, Turvey A. Bacteria associated with the intestinal wall of the fowl (Gallus domesticus) [J]. Journal of Applied Bacteriology, 1971, 34:617-622.
[149] Barrow P A, Brooker B E, Fuller R, et al. Thea attachment of bacteria to the gastric epithelium of the pig and its importance in the microecology of the intestinal[J]. Journal of Applied Bacteriology, 1980, 48:147-154.
[150] Fujiwara S, Hashiba H, Hirota T, et al. Proteinaceous factor(s) in culture supernatant fluids of bifidobacteria which prevents the binding of enterotoxigenic Escherichia coli to gangliotetraosylceramide[J]. Appl. Envir. Microbiol., 1997, 63:506-512.
[151] 王丽娟.效力生素的研究及应用进展[J].饲料博览,1999,(1):15-18.
[152] Mack D R, Michail S, Wei S, et al. Probiotics inhibit erteopathogenic E.Coli adherence in vitro by inducing intestinal mucin gene expression [J]. Smerican Journal of Physiology, 1999, 276(39):15-18.
[153] 田允波,葛长荣,张曦,等.益生素的研究与应用现状[J].兽药与饲料添加剂,1999, 4(1):37-40;(2):35-40;(3):36-39.
[154] 吕道俊,潘康成.微生态制剂对猪细菌性疾病的防治研究进展[J].饲料工业, 1999, 20(10):42-44.
[155] 闵晓梅,孟庆翔.动物胃肠道正常微生物与活菌制剂在猪生产中的应用[J].养猪, 2001, (1):9-12.
[156] Kang K H, et al. Studies on antibacterial substances producted by lacticacid bacteria: Purification and some properties of antibacterial substances ‘Bifilong’ producted by Bifidobacterium longum [J]. Korean J Dairy Sci, 1989, 11(3):204-216.
[157] Pollard M, Sharon N. Resportese of the peyerˊs in germ free mice to angyenic stimulation [J]. Infect Immune, 1970, (2):96-100.
[158] Nahashon S N, Nakaue H S, Snyder S P, et al. Performance of single comb White Leghorn layers fed corn-soybean meal and barley-corn-soybean meal diets supplemented with a direct-fed microbial [J]. Poult Sci, 1994,73(11):1712-1723.
[159] Marie J F, Uassilia T, et al. Boseed systemic immune and local restonsiveness after intestinal inflammation in orally sensitized guinea pigs [J]. Gastro, 1995, 109:53-62.
[160] Duchman R, Kaiser I, Herman E, et al. Tolerance exists towards resident intestinal flora but is broker in active inflammatory bowel disease (IBD) [J].Clin Exp Immunnal, 1995, 102:448-455.
[161] Isolaurie E, Majamaa H, et al. Lactobacilli casei strain GG reverses increased intestinal permeability induced by cow milk in sucking rats [J]. Gastroenterol, 1993, 105:1643-1650.
[162] 赖新峰,王立生,潘令嘉等.双歧杆菌对裸鼠的腹腔巨噬细泡激活作用的初步观察[J].中国微生态学杂志,1999,11(6):336-337.
[163] Sekine K, Ohta J, et al. Analysis of antitumor properites of effecter cells stimulated with a cell wall prepatation (WPG) of bifidobacterimu infantis[J].Biol Pharm Bull, 1995, 18:148-153.
[164] Kota I, Yokokura T, Mutai M. Augmentation of mouse natural killer cell activity by Lactobacillus casei and its surface antigens [J]. Microbiol Imminol, 1984, 27:209-217.
[165] Schiffrin E, Rochat F, Link A H, et al. Immune modulation of human blood guoup following the ingestion of lacticacid bacteria[J]. Journal of Dairy Science, 1995, 78:491-497.
[166] 王魁.分泌型IgA 及其在粘膜局部免疫抗感染中的研究现状[J].中国兽医科技,1998, 21(8):18-22.
[167] 杨倩.巴氏杆菌弱毒苗和鸡新城疫弱毒苗饮水免疫蛋鸡后分泌型A 细胞的分布[J].中国畜禽传染病,1998,20(6):340-342.
[168] German A J, Hall E J, Day M J. Relative deficiency in IgA production by duodenal explants from German shepherd dogs with small intestinal disease [J]. Vet Immunol Immunopathol, 2000, 76(1-2):25-43.
[169] 潘康成, 吕道俊,何明清,等.正常菌群对动物免疫功能的影响研究进展[J].饲料工业,1999,29(9):24-26.
[170] 刘克琳,何明清.益生菌对鲤鱼免疫功能影响的研究[J].饲料工业,2000,21(6):24-25.
[171] 柴家前,孙明军,杨倩.短乳杆菌的分离鉴定及其对鸡肠粘膜SIgA 分泌的影响[J].中国兽医学报, 2001,21(5):485-488.
[172] Perdigon G, Alvarez S, Ruiz H P. Immunoadjuvant activity of oral Lactobacillus casei: influence of dose on the secretory immune response and protective capacity in intestinal infections [J]. J. Dairy. Res., 1991, 58(4):485-496.
[173] Perdigon G, Alvarez S, Rachid M, et al. Immune system stimulation by probiotics[J]. J. Dairy. Sci., 1995, 78:1597-1606.
[174] Yasui H, Nagaoka N, Hayakawa K. Augmentation of anti-influenza virus hemagglutinin antibody production by Peyer's patch cells with Bifidobacterium breve YIT4064[J]. Clin. Diagn. Lab. Immunol., 1994,(1):244-246.
[175] Alvarez S, Gobbato N, Bru E, et al. Specific immunity induction at the mucosal level by viable Lactobacillus casei: Aperspective for oral vaccine development[J]. Food & Agri Immunol, 1998, (10):79-87.
[176] Inooka S, Uehara S, Kimura M. The effect of Bacillus natto on the T and B lymphocytes from spleens of feeding chickens [J]. Poult Sci, 1986, 65(6):1217-1219.
[177] Matsuzaki T, Nagata Y, Kado S, et al. Effect of oral admimistration of Lactobacillus Casei on alloxan induced diabetes in mice [J]. Apmis, 1997, 105:637-642.
[178] Ouwehand A C, Kirjavainen P V, Shortt C, et al. Probiotics: mechanisms and established dffects [J].International Dairy Journal,1999,(9):43-52.
[179] Perdigon G, Macias M E, Alvarez S, et al. Systemic augmentation of the immune response in mice by feeding fermented milks with Lactobacillus casei and Lactobacillus acidophilus[J]. Immunology, 1988,63(1):17-23.
[180] Kitazawa H, Nomura M, Itoh T, et al. Functional alteration of macrophages by a slime-forming Lactococcus lactis ssp. Cremoris[J]. J. Dairy. Sci., 1991, 74:2082-2088.
[181] Kitazawa H, Yamaguchi T, Itoh T. B-cell mitogenic activity of slime products produced from slime-forming, encapsulated Lactococcus lactis ssp. Cremoris [J]. J. Dairy. Sci., 1992, 75:2946-2951.
[182] Berger F M, Fukui G M, Gustafson R H, et al. tudies on the mechanism of protodyne-induced protection against microbial infections [J]. Proc. Soc. Exp. Biol. Med., 1971, 138:391-395.
[183] Himanen J P, Pyhala L, Olander R M, et al. Biological activities of lipoteichoic acid and peptidoglycan-teichoic acid of Bacillus subtilis 168(Marburg) [J]. J Gen Microbiol, 1993, 139(Pt11):2659-2665.
[184] 蓝景刚,胡宏.双歧杆菌及其表面分子的免疫增强作用[J].中国微生态学杂志, 1999, 11(3):129-131.
[185] Yamazaki S, Machii K,Tsuyuki S, et al. Immunological responses to monoassociated Bifidobacterium longum and their relation to prevention of bacterial invasion [J]. Immunology, 1985, 56(1):43-50.
[186] Kopp E, Medzhitov R, Carothers J, et al. ECSIT is an evolutionarily conserved intermediate in the Toll/IL-1 signal transduction pathway [J]. Genes & Dev., 1999, 13:2059-2071.
[187] Rowland T R, Grasso P. High performance liquid cheomatographic determination of Bifidobacterium growth factors in human milk [J].Appl Microbio,1975,29(1):7-17.
[188] 李宝玉,李德发.双歧杆菌和乳酸杆菌对不同碳水化合物利用能力[J].中国饲料,1999,(18):9-10.
[189] Mitsuo K. Effect of long-trem intake of Neosugar Ron intestinal flora and serum lipids[M]. Tokyo, Japan: 1986:235.
[190] Bailey J S, Blankenship L C, Cox N A, et al. Effect of fructooligosaccharide on Salmonella colonization of the chicken intestine [J].Poult Sci, 1991,70(12): 2433-2438.
[191] Firon N, Ashkenazi S, Mirelman D, et al. Aromatic alpha-glycosides of mannose are powerful inhibitors of the adherence of type 1 fimbriated Escherichia coli to yeast and intestinal epithelial cells [J].Infect. Immun., 1987, 55(2): 472-476.
[192] Morgan A, Jetal. Dietary oligosaccharides [J]. Agro. Food industry hig-tech, 1992, 11:35-38.
[193] Tomoyuki Sako, et al. Recent progress on research and applications of non-digestible galacto-oligosaccharides [J]. International Dairy Journal, 1999, (9):69-80.
[194] Pustai A. Chemica probiosis-in recent advances in animal nutrition [M]. London: Butteworth, 1990:47-60.
[195] Newman K. Mannan-oligosaccharides: natural polymers with significant impact on the gastroinvestinal microflora and the immune system [M]. UK: Nottingham University press, 1995:167-174.
[196] 李小宁.魔芋甘露低聚糖口服液对雌性ICR 小鼠免疫功能的调节作用[J].江苏预防,1998,(4):3-4.
[197] 刘福君,赵修南,聂伟,等.地黄低聚糖增强小鼠免疫功能的作用[J].中国药理学通报,1998,14(1):90.
[198] 黄小红,林藩平.甘露聚糖-寡糖对断奶仔猪腹泻的防制[J].中国预防兽医学报(增刊),2000,(22)9:163-165.
[199] Kim D H, Lee S W, Han , et al. Biotransformation of glycyrrhizin to 18-beta-glycyrrhetinic acid-3-O-beta-D-glucuronide by Streptococcus LJ-22, a human intestinal bacterium[J]. Biol. Pharm. Bull, 1999,22(3):320-322.
[200] 左风,严梅桢,周钟鸣.肠道菌群对中药有效成分代谢作用的研究进展[J].中国中药杂志,2002,27(8): 568-572,616.
[201] 田碧云.阿胶、五味子、刺五加、枸杞对双歧杆菌生长的影响[J].中国徽生态学杂志, 1996, 8(2):11-13.
[202] 刘锡葵,李海舟,戴诗梅,等. 植物配糖体对人肠道厌氧菌群的影响[J].中国微生态学杂志,2000,12(4):201-202.
[203] 丁珂.益生乳酸杆菌的筛选及中草药协同作用的研究[D].成都:四川农业大学,2003.
[204] 张东.益生素和中药添加剂对提高断奶仔猪生产性能的作用及机理研究[D].太谷:山西农业大学,2001.
[205] 吴力克,梁冰,熊德鑫等.复合益生菌制剂“海生元”研制初步总结报告[J].中国微生态学杂志,2003,15(4):194-195.
[206] 赵文,任永风,樊建航.伊犁黄芪与蒙古黄芪成分的比较及多糖含量的测定[J].中草药,2001,32(2):122-124.
[207] 吴亚林,黄静,潘远江.无花果多糖的分离L 纯化和鉴定[J].浙江大学学报(理学版),2004,31(2):177-180.
[208] 余晓斌,尤蓉.云芝菌丝体多糖的分离纯化研究[J].天然产物研究与开发,2003,15(1):29-31.
[209] 刘星谐,王美英,吴厚生,等.黄芪多糖的分离及其免疫活性的研究[J].天然产物研究与开发,1994,16(1):23.
[210] 邹一愚,顾学裘,陈骐,等.双多糖多相脂质体的研究(1)多糖中有效成分的选择和鉴定[J]. 沈阳药学院学报,1998,4(3):170.
[211] 方积年.黄芪葡聚糖的化学结构[J]. 化学学报,1988,46:1101.
[212] 李健强,李六金.兽医微生物学实验实习指导[M].西安:科学技术出版社,1999.
[213] 余萍,林曦,刘艳如.水溶性低聚壳聚糖、壳聚糖和山苍籽油抑菌效果比较[J].药物生物技术,1999,6(3):171-173.
[214] 邵健,杨宇民,姚成.低聚氨基葡萄糖的吸湿、保湿和抑菌性质[J].中国海洋药物,2000, (4):25-27.
[215] Nurmi E, Rantala M. New aspects of Salmonella in broiler production [J]. Nature, 1973, 214:210-211.
[216] Lomberg L B, Enriksson A H, Comvay P L. Inhibition of Adhesion of Escherichia coli K88 to Piglet Ileal Mucus by lactobacillus spp [J].Appland Environ Microbi, 1993,34-39.
[217] Pascual M, Hugas M, Badiola J I, et al. Lactobacillus salivarius CTC2197 prevents salmonella enteritis discolonization in chick-ens [J]. Appli and Environ Microbi, 1999.
[218] Dahiya R S, Speck M L. Hydrogen peroxide formation by lactobacilli and its effect on Staphylococcus aureus [J]. J. Dairy. Sci., 1968.
[219] 王红宁,何明清,柳苹,等.鲤肠道正常菌群的研究[J].水生生物学报,1994,18(4):354-359.
[220] 程安春,何明清,陈孝跃. SA_38 蜡样芽孢杆菌在体外对几种致病菌的生物拮抗试验[J].中国兽医杂志,1994,20(2):12-13.
[221] Jwven B J, Weiss l H, Herel S. Dectition of hydrogen peroxide produce by meat lactic starter culture [J]. J. Appli Bactri, 1988, 65:357-360.
[222] Sorrels K M, Speck M L. Inhibition of Salmonella gallinarum by culture filtrates of Leuconost or citrovorum [J].J. Dairy Sci, 1970, 53:237-343.
[223] Fuller R. Probiotics in man and animal [J].J Appli Bacteri, 1989,6:365-378.
[224] Reid G, McGroarty J A, Angotti R, at al.Lactobacillus inhibitor production against Escherichia coli and coaggregation ability with uropathogens[J].Can J Microbiol, 1988, 34(3): 344-351.
[225] Juven B J, Meinersmann R J, Stern N J. Antagonistic effects of lactobacilli and pediococci to control intestinal colonization by human enteropathogens in live poultry [J]. J Appl Bacteriol, 1991,70(2): 95-103.
[226] 曹澎泽.兽医微生物学与免疫学试验[M].北京:农业出版社,1985.
[227] 沈关心,周汝麟.现代免疫学实验技术[M].武汉:湖北科学技术出版社,1998.
[228] 陈天寿.微生物培养基的制造与应用[M].北京:中国农业出版社, 1995.
[229] R.E.希坎南,N.E.吉布斯等.伯杰氏细菌鉴定手册(第八版)[M].北京:科学出版社, 1984.
[230] 何明清,程安春.动物微生态学[M].成都:四川科学技术出版社, 2004.
[231] Abdulrahim S M, T.Haddadin M S, Odetallah N H M, et al. Effect of Lactobacillus acidophilus and zinc bacitracin as dietary additives for broiler chickens. Brit Poult Sci.1999.40:91-94
[232] Jamas, Spiros, Easson, et al. Use for underivatized aqueous soluble beta-(1,3)glucan and composition comprising same. United States Patents: 5783569, 1998-7-21.
[233] 王道苑,杨蔚怡.黄芪多糖对小鼠肝细胞核酸代谢的影响[J].中国药理学报,1982,3(8):204.
[234] Cummings J H. The large intestine in nutrition and disease. Brussels: Institute Danone,1997:15-42
[235] 余成瑶,郑黎,周毅.肉鸡饲喂益生素后小肠黏膜上皮细胞肝细胞超微结构功能研究扫描电镜下的研究.四川农业大学学报,1994,14(1):61-66,56.
[236] 余成瑶,何明清,郑德成.肉用仔鸡饲喂微生物饲料添加剂(8901)细胞碱性磷酸酶(AKP)定性、定位研究.四川农业大学学报,1994,12(增刊):596-601.
[237] 余成瑶.肉用仔鸡饲喂微生物饲料添加剂(8901)细胞多糖的定性、定位研究.四川农业大学学报,1994,12(增刊):588-595
[238] Wilson K H,Perini F. Role of competition for nutrients in suppression of Clostridium difficiel by the colonic microflora [J].Infection and Immunity, 1988, 56:2610-2614.
[239] Fuller R, Turvey A. Bacteria associated with the intestinal wall of the fowl (Gallus domesticus) [J]. Journal of Applied Bacteriology, 1971, 34:617-622.
[240] Barrow P A, Brooker B E, Fuller R, et al. Thea attachment of bacteria to the gastric epithelium of the pig and its importance in the microecology of the intestinal[J]. Journal of Applied Bacteriology, 1980, 48:147-154.
[241] 吕道俊, 潘康成. 微生态制剂对猪细菌性疾病的防治研究进展[J]. 饲料工业,1999,20(10):42-44.
[242] 闵晓梅, 孟庆翔. 动物胃肠道正常微生物与活菌制剂在猪生产中的应用[J]. 养猪,2001,(1):9-12.
[243] 李兰娟.感染微生态学[M].北京:人民卫生出版社,2002.
[244] 阴天榜,刘兴友.家禽免疫学[M].北京:中国农业科技出版社,1998.
[245] Matutes E and Catovsky D. The fine structure of normal lymphocyte subpopulations--a study with monoclonal antibodies and the immunogold technique [J]. Clin Exp Immunol, 1982, 50(2): 416-25.
[246] 郭峰,姚广金,孟祥英.血清中红细胞免疫促进因子的测定[J].上海免疫学杂志,1988,8(6):440
[247] 刘景田,张洁,党小军.血清对粒细胞免疫调节作用的研究[J].中国免疫学杂志,1995,11(增刊):209 [248] 刘景田,张洁.红细胞免疫学[M].西安:陕西科技出版社,1995:15-19,32-34.
[249] 郭峰.红细胞调控细胞免疫的事实与意义[J].上海免疫学杂志,1991,11(1):63.
[250] Yannelli J R, Thurman G B, Bastin A M, et al. Enhancement of human lymphokine-activaed killer cell cytolisis and o method for increasing lymphokine-activated killer cell yields to cancer patients [J].Cancer Res., 1988,48(20):56-96.
[251] 郭兴华主编.益生菌基础与应用[M].北京:北京科学技术出版社,2002.
[252] 罗克.家禽解剖学与组织学[M]. 福州:福建科学技术出版社,1983.
[253] 倪耀娣,李睿文,鲁改如,等.微生态制剂对肉仔鸡小肠结构的影响[J].江西畜牧兽医杂志,2004,(4):7-8.