泥螺对多环芳烃生物可利用性的初步研究
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摘要
PAHs作为天津海岸带的典型污染物质,因具有持久性、生物难降解性及高毒性,对环境中的生物及人类健康造成严重的威胁,导致河口及其海岸带生境退化,目前人们逐渐意识到采取有效措施对生态环境进行修复的必要性和紧迫性。因此,调查有代表性潮间带沉积物及生物体泥螺中PAHs含量,并研究泥螺对PAHs的生物可利用性及沉积物中PAHs的解吸行为,对评价受污染沉积物的生态风险、选择合理的修复技术及制定合理的修复策略都具有十分重要的意义。
本文选取受PAHs污染且有代表性的潮间带为生态示范区,采用气相色谱-质谱联用仪(GC-MS)研究了该区表层沉积物及优势种泥螺体内PAHs含量及分布特征。结果表明,沉积物和泥螺体内均以二环和三环萘、芴、菲、蒽为主,根据Long提出的沉积物风险评价标准,萘、芴、菲、蒽超出风险低值,对潮间带生态具有潜在危害,其中石油输入是主要污染源,并混有化石燃料的不完全燃烧。
以菲为PAHs代表污染物,通过室内模拟试验探讨其对泥螺的毒性效应及生物累积效应,通过测定BSAF(Biota-Sediment Accumulation Factor)值研究泥螺对菲的生物可利用性。结果表明,菲对泥螺7d的理论LC_(50)值为73.6mg/kg,且LC_(50)随污染暴露试验时间的延长而减小;泥螺对不同浓度水平菲的BSAF值不同,随着菲污染水平的升高,泥螺体内菲累积量显著增大。新鲜染毒的菲易被生物利用,BSAF值＞1,而老化100 d沉积物中的菲被“锁定”,减缓它的释放速率,导致其生物可利用性降低,BSAF值＜1。
采用循环吸附/解吸实验法,研究了菲在沉积物中的吸附和解吸行为,并用DED(Dual-Equilibrium Desorption)模型对解吸行为进行了预测。结果表明,菲在沉积物中的吸附符合传统线性模型,解吸曲线明显偏离吸附曲线,存在不可逆解吸现象,且DED模型的预测值与实验结果相吻合。
总之,研究泥螺对PAHs的生物可利用性,可以为河口及潮间带的环境监测、生态风险评价及生物修复提供科学依据。
As one of typical persistent pollutants in Tianjin's coastal zone, PAHs have the properties of persistence, bio-refractory and high toxicity, which has caused serious harm to environment, organism and human health. Now people gradually are realizing the necessity and urgency of taking effective measures to restore damaged ecological environment. Therefore, quantitatively analyzing the concentrations and distribution of PAHs in surface sediments and dominant species Bullacta exarata sampled from the typical intertidal zone, studying on the bioavailability of PAHs to Bullacta exarata and desorption behaviors of PAHs in sediment have great significance for ecological risk assessment, selecting reasonable remediation technologies and making feasible reparation strategy.
Concentrations and distribution of 10 PAHs in surface sediments and Bullacta exarata sampled from the typical damaged intertidal zone were quantitatively analyzed by GC/MS, the main polluted sources in this area were discussed. The results showed that the concentrations of 2,3 ringed PAHs compounds in all detected PAHs were the most dominant both in sediments and organisms. The main source of PAHs in the studying area might be petroleum-derived contamination mixing incomplete combustion of the fossil fuel. According to sediment quality criterion, the concentration of naphthalene, fluorene, phenanthrene and anthracene exceed the risk low (ERL) values, which might be a certain potential toxic effect to the ecosystem of intertidal zone.
The acute toxicity and bioaccumulation of Phe selected as a representative PAHs to Bullacta exarata was studied by simulation experiments in lab. The testing result showed that the theoretical median lethal concentration (LC50) value of Phe to Bullacta exarata was 73.6 mg/kg and the LC50 value was decreased with the increase of exposure time. At different contamination levels of Phe, the BSAF values were various for Bullacta exarata. With Phe concentration increasing in sediments, the accumulation of Phe in Bullacta exarata was increases significantly. Phe fresh spiked in sediments was biological utilized easily correspondingly with BSAF>1, when Phe in sediemnt was aged for 100d, Phe would be sequestrated by organic matters, which slowed down the releasing rate and reduced the bioavailability, so BSAF was less than 1.
The sorption/desorption behavior of Phe in sediments from ecological demonstration area were studied using multiple cycles of sorption/desorption experiments and the desorption data were predicted using a new desorption model 'Dual-Equilibrium Desorption (DED) model'. The result showed that the sorption of both naphthalene and Phe agree well with the conventional linear sorption isotherm. While the desorption curve which showed an irreversible phenomenon was deviation from sorption isotherm but it could be predicted well with the DED model.
In summary, this paper had a preliminary study on the bioavailability of PAHs contaminants to Bullacta exarata, which provided scientific basis for the environmental monitoring, ecological risk assessment and bioremediation of estuary and intertidal zone.
本文选取受PAHs污染且有代表性的潮间带为生态示范区,采用气相色谱-质谱联用仪(GC-MS)研究了该区表层沉积物及优势种泥螺体内PAHs含量及分布特征。结果表明,沉积物和泥螺体内均以二环和三环萘、芴、菲、蒽为主,根据Long提出的沉积物风险评价标准,萘、芴、菲、蒽超出风险低值,对潮间带生态具有潜在危害,其中石油输入是主要污染源,并混有化石燃料的不完全燃烧。
以菲为PAHs代表污染物,通过室内模拟试验探讨其对泥螺的毒性效应及生物累积效应,通过测定BSAF(Biota-Sediment Accumulation Factor)值研究泥螺对菲的生物可利用性。结果表明,菲对泥螺7d的理论LC_(50)值为73.6mg/kg,且LC_(50)随污染暴露试验时间的延长而减小;泥螺对不同浓度水平菲的BSAF值不同,随着菲污染水平的升高,泥螺体内菲累积量显著增大。新鲜染毒的菲易被生物利用,BSAF值＞1,而老化100 d沉积物中的菲被“锁定”,减缓它的释放速率,导致其生物可利用性降低,BSAF值＜1。
采用循环吸附/解吸实验法,研究了菲在沉积物中的吸附和解吸行为,并用DED(Dual-Equilibrium Desorption)模型对解吸行为进行了预测。结果表明,菲在沉积物中的吸附符合传统线性模型,解吸曲线明显偏离吸附曲线,存在不可逆解吸现象,且DED模型的预测值与实验结果相吻合。
总之,研究泥螺对PAHs的生物可利用性,可以为河口及潮间带的环境监测、生态风险评价及生物修复提供科学依据。
As one of typical persistent pollutants in Tianjin's coastal zone, PAHs have the properties of persistence, bio-refractory and high toxicity, which has caused serious harm to environment, organism and human health. Now people gradually are realizing the necessity and urgency of taking effective measures to restore damaged ecological environment. Therefore, quantitatively analyzing the concentrations and distribution of PAHs in surface sediments and dominant species Bullacta exarata sampled from the typical intertidal zone, studying on the bioavailability of PAHs to Bullacta exarata and desorption behaviors of PAHs in sediment have great significance for ecological risk assessment, selecting reasonable remediation technologies and making feasible reparation strategy.
Concentrations and distribution of 10 PAHs in surface sediments and Bullacta exarata sampled from the typical damaged intertidal zone were quantitatively analyzed by GC/MS, the main polluted sources in this area were discussed. The results showed that the concentrations of 2,3 ringed PAHs compounds in all detected PAHs were the most dominant both in sediments and organisms. The main source of PAHs in the studying area might be petroleum-derived contamination mixing incomplete combustion of the fossil fuel. According to sediment quality criterion, the concentration of naphthalene, fluorene, phenanthrene and anthracene exceed the risk low (ERL) values, which might be a certain potential toxic effect to the ecosystem of intertidal zone.
The acute toxicity and bioaccumulation of Phe selected as a representative PAHs to Bullacta exarata was studied by simulation experiments in lab. The testing result showed that the theoretical median lethal concentration (LC50) value of Phe to Bullacta exarata was 73.6 mg/kg and the LC50 value was decreased with the increase of exposure time. At different contamination levels of Phe, the BSAF values were various for Bullacta exarata. With Phe concentration increasing in sediments, the accumulation of Phe in Bullacta exarata was increases significantly. Phe fresh spiked in sediments was biological utilized easily correspondingly with BSAF>1, when Phe in sediemnt was aged for 100d, Phe would be sequestrated by organic matters, which slowed down the releasing rate and reduced the bioavailability, so BSAF was less than 1.
The sorption/desorption behavior of Phe in sediments from ecological demonstration area were studied using multiple cycles of sorption/desorption experiments and the desorption data were predicted using a new desorption model 'Dual-Equilibrium Desorption (DED) model'. The result showed that the sorption of both naphthalene and Phe agree well with the conventional linear sorption isotherm. While the desorption curve which showed an irreversible phenomenon was deviation from sorption isotherm but it could be predicted well with the DED model.
In summary, this paper had a preliminary study on the bioavailability of PAHs contaminants to Bullacta exarata, which provided scientific basis for the environmental monitoring, ecological risk assessment and bioremediation of estuary and intertidal zone.
引文
[1]Sicre M A,Marty J C,Saliot A,et al.Aliphatic and aromatic hydrocarbons in Mediterranean aerosol[J].International J.of Environ.Anal.Chem,1987,29:73-94.
[2]滕彦国,倪师军,林学钰,等.城市环境地球化学研究综述[J].地质论评,2005,51(1):64-76.
[3]张宗雁.持久性有机污染物在东海泥质沉积区的分布与沉积记录[D].山东:中国海洋大学,2002.
[4]王连生,孔令仁,韩朔睽.致癌有机物[M].北京:中国环境科学出版社,1-41.
[5]罗世霞.红枫湖水体和沉积物中有机污染物.多环芳烃的污染现状及源解析研究[D].贵州:贵州师范大学,2005.
[6]Baumard P,Budzinski H,Michon Q,et al.Origin and bioavailability of PAHs in the Mettettanean Sea from mussel and sediment records[J].Estuarine,Coastal and Shelf Science,1998,47(1):77-90.
[7]王玉萍,苏克曼,胡慧廉,等.苏州河底质中有机污染物的定性鉴定[J].中国环境科学,1992,12(2):145-149.
[8]Qu W C,Mike D,Wang S,et al.Evidence for an aquatic plant origin of ketones found in Taihu Lake sediments[J].Hydrobiologia,1999,397:149-154.
[9]周永武,胡慧廉,施月儿,等.苏州河底质中优先有机污染物的定量测定方法研究[J].中国环境科学,1992,12(5):343-347.
[10]Wang X H,Xu L,Chen W Q,et al.The vertical distribution and sources of PAHs in sediment of Xiamen Bay[J].Chin J Oceanol Limnol,1999,17(3):247-251.
[11]Trapido M.Polycyclic aromatic hydrocarbons in Estonian soil:contamination and profiles[J].Environmental Pollution,1999,105:67-74.
[12]Danuta B,Beata J.Comparison ofpolycyclic aromatic compounds and heavy metals contents in sewage sludges from Industrialized and non-Industrialized Region [J].Water Air and Soil Pollution,1998,106:359-369.
[13]陈楚良.超临界流体萃取法[J].上海环境科学,1994,13(4):19-21.
[14]Dietmar K,Martin S,Virpi V,et al.Determination of polycyclic aromatic hydrocarbons in contaminated' water and soil samples by Immunological and Chromatogra-phicmethods [J].Environmental Science and Technology,2000,34:2035-2041.
[15]Antonio C,Emilien P,Cristian L,et al.Polycyclic aromatic hydrocarbons in soil and surface marine sediment near Jubany Station(Antarctica).Role of permafrost as a low-permeability barrier[J].Science of the Total Environment,2007,383:193-204.
[16]丁爱芳,潘根兴,李恋卿.江苏省部分地区农田表土多环芳烃含量比较及来源分析[J].生态与农村环境学报,2007,23(2):71-75.
[17]高春梅.大气中多环芳烃的分布规律[J].环境科学,1993,5(1):79-81.
[18]马西林,丁中华,刘瑞莲,等.大同市空气中多环芳烃与人尿中1-羟基芘的分析[J].环境化学,1994,14(6):536-542.
[19]成玉,盛国英,傅家谟,等.大气气溶胶中多环芳烃的定量分析[J].环境化学,1996,15(4):360-365.
[20]Nielsen T,Jorgensen H E,Larsen J C,et al.City air Pollution ofpolycyclic aromatic hydrocarbons and other mutages:occurrence,sources and health effects[J].Sci.Total Environ,1996,190:41-49.
[21]沈晓平.南通市地面水中多环芳烃的调查[J].南通医学院报,1994,14(3):408-409.
[22]康君行,赵立文.高层建筑水箱浸出有机物的GC/MS分析[J].环境化学,1995,14(3):265-268.
[23]Lee H B,翁建华,黄连芬.超临界二氧化碳萃取:GC/MS测定土壤中的多环芳烃[J].中国环境监测,1996,12(2):9-11.
[24]刘敏,侯立军,邹惠仙,等.长江口潮滩表层沉积物中多环芳烃分布特征[J].中国环境科学,2001,21(4):343-346.
[25]刘现明,徐学仁,张笑天,等.大连湾沉积物中PAHs的初步研究[J].环境科学学报,2001,21(4):507-509.
[26]林秀梅,刘文新,陈江麟,等.渤海表层沉积物中多环芳烃的分布与生态风险评价[J].环境科学学报,2005,25(1):70-75.
[27]杨永亮,麦碧娴,潘静,等.胶州湾表层沉积物中多环芳烃的分布及来源[J].海洋环境科学,2003,22(4):38-43.
[28]田蕴,郑天凌,王新红.厦门西港表层沉积物中多环芳烃(PAHs)的含量、分布及来源[J].海洋与湖沼,2004,35(1):15-20.
[29]麦碧娴,林峥,张干,等.珠江三角洲河流和珠江口表层沉积物中有机污染物研究-多环芳烃和有机氯农药的分布特征[J].环境科学学报,2000,20(2):192-197.
[30]Yan L,Pui K F,Man F T,et al.Sources and seasonal variation of PAHs in the sediments of drinking water reservoirs in Hong Kong and the Dongjiang River(China)[J].Environ Monit Assess,2002,133:447-458.
[31]江锦花,丁理法.台州湾五种海洋生物体内多环芳烃的浓度、富集特征及环境效应[J].环境污染与防治,2007,29(5):394-397.
[32]Kan H,Leinemann M.Determination of polycyclic aromatic hydrocarbons in smoked fisherf products from different smoking kilns[J].Z.Lebensm.Unters Forsh,1996,202(6):458-464.
[33]Madhavan N D,Naidu K A.Polycyclic aromatic hydrocarbons in placenta,maternal blood,umbilical card blood and milk of Indian women[J].Hum.Exp.Toxicol,1995,14(6):503-506.
[34]Juhasz A L,Meghara J M,Naidu R.Bioavailability:the major challenge(constraint)to bioremediation of organically contaminated soils[A].In:WISE D L,et al.Biore-mediatiom of Contaminated Soils[C].New York:Marcel Dekker Inc,2000:217-241.
[35]Chung N,Alexander M.Effect of soil properties on bioavailability and extractability of phenanthrene and atrazine sequestered in soil[J].Chemosphere,2002,48:109-115.
[36]Rittmann B E,Mccarty P L.Environmental biotechnology:principles and applications [M].New York:McGraw-Hill Com Inc,2001:695-730.
[37]Lu X X.Bioavailability and bioaccumulation of sediment-associated,desorption-resistant fraction of polycyclic aromatic hydrocarbon contaminants[D].USA:Louisiana State University and Agricultural and Mechanical College,2003.
[38]Richard G,Luthy,Richelle M,et al.Bioavailability of contaminants in soils and sediments[M],2002.
[39]Reid B J,Jones H C.Bioavailability of persistent organic pollutants in soils and sediments-a perspective on mechanisms,consequences and assessment[J].Environ Pollution,2000,108:103-112.
[40]Hatzinger P B,Alexander M.Effect of ageing of chemicals in soil on their biodegradability and extractability[J].Environ Sci Technol,1995,29:537-545.
[41]Theng B K,Aislabie J,Fraser R.Bioavailability of phenanthrene intercalated into analkylammonium-montmorillonite clay[J].Soil Biol Biochem,2001,33:845-848.
[42]Tang J,Liste H,Alexander M.Chemical assays of availability to earthworms of polycyclic aromatic hydrocarbons in soil[J].Cheosphere,2002,48:35-42.
[43]Johnson D L,Jones K C,Langdon C J,et al.Temporal changes in earthworm availability and extractability of polycyclic aromatic hydrocarbons in soil[J].Soil Biology and Biochemistry,2002,34:1363-1370.
[44]Lu X X,Danny D,Beible,et al.Biovailability of desorption-resistant Phenanthrene to the Oligochaete Ilyodrilus ternpletoni[J].Environmental Toxicology and Chemistry,2003,22(1):153-160.
[45]陶玉强,张淑贞,许宜平,等.利用三油酸甘油酯-醋酸纤维素复合膜评价土壤中多环芳烃的生物可利用性[J].生态毒理学报,2007,2(3):284-291.
[46]Pignatello J J,Xing B.Mechanisms of slow sorption of organic chemicals to natural particles[J].Environmental Science and Technology,1996,30,1-10.
[47]Beckles,D M.Bioavailability of polycyclic aromatic hydrocarbons as sediment associated desorption-resistant contaminants[D].Houston:Rice University,2000.
[48]Karickhoff S W,Brown D S,Scott T A.Sorption of hydrophobic pollutants on natural sediment[J].Water Research,1979,13,241-248.
[49]Karickhoff S W.Semi-empirical estimation of sorption of hydrophobic pollutants on natural sediments and soils[J].Chemosphere,1981,10,833-846.
[50]Means J C,Wood S G,Hassett J J,et al.Sorption of polynuclear aromatic hydrocarbons by sediments and soils[J].Environmental Science and Technology,1980,14,1524-1528.
[51]Pereira,W E,Rostad,C E,Chiou,C T,et al.Contamination of estuarine water,biota,and sediment by helogenated organic compounds:a field study[J].Environmental Science and Technology,1988,27:772-778.
[52]Readman,J W,Mantoura,R F,Rhead,M M.A record of polycyclic aromatic hydrocarbons(PAH) pollution obtained from accreting sediments of the Tamar estuary,U K:evidence for non-equilibrium behavior of PAH[J].Science of Total Environmeny,1987,66:73-94.
[53]Chen,W,Kan,A T,Fu G,et ai.Adsorption-desorption behaviors ofhydrophobic organic compounds in sediments of lake Charles,Louisiana,USA[J].Environmental Toxicology and Chemistry,1999,18,1610-1616.
[54]Kan A T,Fu G,Hunter M,et al.Irreversible adsorption of neutral organic hydrocarbons-Experimental observations and modelpredictions[J].Environmental Science and Technology,1998,32:892-902.
[55]Chen W,Kan A T,Tomson M B.Irreversible adsorption of chlorinated benzenes to natural sediments-implication for sediment quality criteria[J].Environ Sci Technol,2000,34:385-392.
[56]Landrum P F,Robbins J A.Bioavailability of sediment-associated contaminants to benthic invertebrates[J].Sediments:Chemistry and Toxicity of In-Place Pollutants,1990,23:237-263.
[57]黄建东,洪华生,等.厦门大学海滨沙滩动力沉积环境特征[J].福建环境,1999,16(3):9-11.
[58]刘宪斌,朱琳,张福,等.天津塘沽驴驹河-独流减河潮间带沉积物特征研究[J].海洋通报,2005,24(3):37-41.
[59]张青田,孟迪,胡桂坤.由渤海海岸带生境修复谈泥螺[J].海洋信息,2006,(1):12-13.
[60]顾晓英,尤仲杰,王一农,等.泥螺Bullacta exarata生长生物学的初步研究[J].浙江水产学院学报,1997,16(1):6-11.
[61]李树国.泥螺的生物学[J].水利渔业,2005,25(4):42-43.
[62]Edgren M.Sediment as indicator of pollution[J].Ambio,1977,5,133-146.
[63]马德毅.海洋沉积物的污染指示作用和检测方法[J].海洋通报,1993,12(5):89-96.
[64]袁兴中.河口潮滩湿地底栖动物群落的生态学研究[D].上海:华东师范大学,2001.
[65]GB/T17378.3-1998,海洋监测规范第3部分:样品采集、贮存与运输[S].北京:海洋出版社,1998.
[66]朱文锋.广东省沿海表层沉积物中多环芳烃研究[D].广东:中国科学院广州地球化学研究所,2007.
[67]GB/T 17378.5-1998,海洋监测规范:沉积物分析[S].北京:国家海洋局,1998.
[68]GB/T13909-1992,海洋调查规范:海洋地质地球物理调查方法[S].北京:国家技术监督局,1992.
[69]Dong R B,Xu D F,Liu L,et al.Behavior ofpolynuclear aromatic hydrocarbons in Environment[J].Environment and Development,1999,14(4):10-12.
[70]吴起航,麦碧娴,杨清书,等.沉积物中多环芳烃和有机氯农药赋存状态[J].中国环境科学,2004,24(1):89-93.
[71]张宗雁,郭志刚,张干,等.东海泥质区表层沉积物中多环芳烃的分布特征及物源[J].地球化学,2005,34(4):379-386.
[72]陈卓敏,高效江,宋祖光,等.杭州湾潮滩表层沉积物中多环芳烃的分布及来源[J].中国环境科学,2006,26(2):233-237.
[73]党志,于虹,黄伟林,等.土壤/沉积物吸附有机污染物机理研究的进展[J].化学通报,2001,2:81-85.
[74]Master D J,MeKee B A,Nittrouer C A,et al.Rates of sediments accumulation and particle reworking based on radiochemical measurements from continental shelf deposits in the East China Sea[J].Continental ShelfRes,1985,4(1-2):143-158.
[75]Christensen M,Andersen O,Banta G T.Metabolism of pyrene by the polychaetes Nereis diversieolor and Arenicola marina[J].AquaToxicol,2002,58:15-25.
[76]Fernandes M B,Sicre M A,Boireau A,et al.Polyaromatic hydrocarbon (PAH)distributions in the Scine River and its estuary[J].Marine Pollution Bulletin,1999,38(1):7-15.
[77]Dickhut R M,Canuel E A,Gustafson K E,et aI.Automatic sources of carcinogenic polycyclic aromatic hydrocarbons associated with particulate matter in the Chesapeake Bay region[J].Environ Sci Technol,2000,34(21):4635-4640.
[78]Witt G.Polycyclic aromatic hydrocarbons in water and sediment of The Baltic Sea[J].Mar Pollut Bull,1995,31(4):237-248.
[79]Long E R,Macdonald D D,Smith S L et al.Incidence of adverse biological effects with ranges of chemical concentrations in marine and estuarine sediments [J].Environmental Management,1995,19(1):81-97.
[80]Durou C,Mouneyrac C,Amiard-Triquet C.Tolerance to metals and assessment of energy reserves in the polychaete Nereis diversicolor in clean and contamined estuaries[J].Environ Toxicol,2005,20:23-31.
[81]Warnau M R,Biondo A,Temara J M,et al.Distribution of heavy metals in the echinoid Paracentrotus lividus from the Mediterranean Posidonia oceanica ecosystem:seasonal and geographical variations[J].Sea Res.,1998,39:267-280.
[82]李丽娜,陈振楼,许世远,等.长江口滨岸潮滩底栖动物泥螺受铜污染的毒理学研究[J].海洋环境科学,2004,23(3):24-27.
[83]李丽娜,陈振楼,许世远,等.长江口滨岸潮滩底栖泥螺受铅污染的急性毒理试验[J].海洋湖沼通报,2005,7(2):88-92.
[84]戴树桂.环境化学[M].北京:高等教育出版社,1985,161-163.
[85]顾晓英,尤仲杰,王一农,等.泥螺Bullacta exarata生长生物学的初步研究[J].浙江水产学院学报,1997,16(1):6-11.
[86]李树国.泥螺的生物学[J].水利渔业,2005,25(4):42-43.
[87]王一农,尤仲杰,於宏,等.养殖泥螺生态习性研究[J].宁波大学学报(理工版),2003,16(3):241-245.
[88]湖南医学院卫生学教研组.卫生毒理实验方法[M].人民卫生出版社,1979,4.
[89]赵作媛.镉-菲复合污染对蚯蚓的急性毒性效应及抗氧化酶的影响[D].上海:上海交通大学,2007.
[90]苗晶晶.多环芳烃化合物对栉孔扇贝致毒机理的研究[D].山东:中国海洋大学,2006.
[91]Lake J L,Rubinstein N I,Pavignano S.Predicting bioaccumulation:Development of a partitioning model for use as a screening tool in regulating ocean disposal of wastes.In Dickson KL,Brungs WA,eds,Fate and effects of sediment-bound chemicals in aquatic systems[M].Pergamon press,New York,USA,1987,151-166.
[92]Ankley G T,Call D J,Hoke R A,et al.Bioaccumulation of PCBs from sediments by Oligochaetes and fishes:Comparison of laboratory and field studies[J].Canadian Journal of Fish Aquatic Science,1992,49:2080-2085.
[93]Millward R N,Fleeger J W,Reible D D.Pyrene bioaccumulation,effects of pyrene exposure on particle-size selection,and fecal pyrene content in the Oligochaete Limnodrilus hoffmeisteri(Tubificide,Oligochaete)[J].Environmental Toxicology and Chemistry,2001,20:1359-1366.
[94]Kraaij R,Mayer P,Busser F J,et al.Measured pore water concentrations make equilibrium portioning work-a data analysis[J].Environmental Science and Technology,2003,37:268-274.
[95]陈华林,陈英旭,王子健,等.中国南方河流和湖泊沉积物对菲的吸附特性[J].环境科学,2003,24(5):120-124.
[96]Alexander M.Aging,bioavailability,and overestimation of risk from environmental pollutants[J].Environ Sci Technol,2002,34(20):4259-4265.
[97]SanderM,Lu Y,Pignatello J J.Conditioning-annealing studies of natural organic matter solids linking irreversible sorption to irreversible structural expansion [J].Environ Sci Technol,2006,40(1):170-178.
[98]罗晓丽,齐亚超,张承东,等.多环芳烃在中国两种典型土壤中的吸附和解吸行为研究[J].环境科学学报,2008,28(7):1375-1380.
[2]滕彦国,倪师军,林学钰,等.城市环境地球化学研究综述[J].地质论评,2005,51(1):64-76.
[3]张宗雁.持久性有机污染物在东海泥质沉积区的分布与沉积记录[D].山东:中国海洋大学,2002.
[4]王连生,孔令仁,韩朔睽.致癌有机物[M].北京:中国环境科学出版社,1-41.
[5]罗世霞.红枫湖水体和沉积物中有机污染物.多环芳烃的污染现状及源解析研究[D].贵州:贵州师范大学,2005.
[6]Baumard P,Budzinski H,Michon Q,et al.Origin and bioavailability of PAHs in the Mettettanean Sea from mussel and sediment records[J].Estuarine,Coastal and Shelf Science,1998,47(1):77-90.
[7]王玉萍,苏克曼,胡慧廉,等.苏州河底质中有机污染物的定性鉴定[J].中国环境科学,1992,12(2):145-149.
[8]Qu W C,Mike D,Wang S,et al.Evidence for an aquatic plant origin of ketones found in Taihu Lake sediments[J].Hydrobiologia,1999,397:149-154.
[9]周永武,胡慧廉,施月儿,等.苏州河底质中优先有机污染物的定量测定方法研究[J].中国环境科学,1992,12(5):343-347.
[10]Wang X H,Xu L,Chen W Q,et al.The vertical distribution and sources of PAHs in sediment of Xiamen Bay[J].Chin J Oceanol Limnol,1999,17(3):247-251.
[11]Trapido M.Polycyclic aromatic hydrocarbons in Estonian soil:contamination and profiles[J].Environmental Pollution,1999,105:67-74.
[12]Danuta B,Beata J.Comparison ofpolycyclic aromatic compounds and heavy metals contents in sewage sludges from Industrialized and non-Industrialized Region [J].Water Air and Soil Pollution,1998,106:359-369.
[13]陈楚良.超临界流体萃取法[J].上海环境科学,1994,13(4):19-21.
[14]Dietmar K,Martin S,Virpi V,et al.Determination of polycyclic aromatic hydrocarbons in contaminated' water and soil samples by Immunological and Chromatogra-phicmethods [J].Environmental Science and Technology,2000,34:2035-2041.
[15]Antonio C,Emilien P,Cristian L,et al.Polycyclic aromatic hydrocarbons in soil and surface marine sediment near Jubany Station(Antarctica).Role of permafrost as a low-permeability barrier[J].Science of the Total Environment,2007,383:193-204.
[16]丁爱芳,潘根兴,李恋卿.江苏省部分地区农田表土多环芳烃含量比较及来源分析[J].生态与农村环境学报,2007,23(2):71-75.
[17]高春梅.大气中多环芳烃的分布规律[J].环境科学,1993,5(1):79-81.
[18]马西林,丁中华,刘瑞莲,等.大同市空气中多环芳烃与人尿中1-羟基芘的分析[J].环境化学,1994,14(6):536-542.
[19]成玉,盛国英,傅家谟,等.大气气溶胶中多环芳烃的定量分析[J].环境化学,1996,15(4):360-365.
[20]Nielsen T,Jorgensen H E,Larsen J C,et al.City air Pollution ofpolycyclic aromatic hydrocarbons and other mutages:occurrence,sources and health effects[J].Sci.Total Environ,1996,190:41-49.
[21]沈晓平.南通市地面水中多环芳烃的调查[J].南通医学院报,1994,14(3):408-409.
[22]康君行,赵立文.高层建筑水箱浸出有机物的GC/MS分析[J].环境化学,1995,14(3):265-268.
[23]Lee H B,翁建华,黄连芬.超临界二氧化碳萃取:GC/MS测定土壤中的多环芳烃[J].中国环境监测,1996,12(2):9-11.
[24]刘敏,侯立军,邹惠仙,等.长江口潮滩表层沉积物中多环芳烃分布特征[J].中国环境科学,2001,21(4):343-346.
[25]刘现明,徐学仁,张笑天,等.大连湾沉积物中PAHs的初步研究[J].环境科学学报,2001,21(4):507-509.
[26]林秀梅,刘文新,陈江麟,等.渤海表层沉积物中多环芳烃的分布与生态风险评价[J].环境科学学报,2005,25(1):70-75.
[27]杨永亮,麦碧娴,潘静,等.胶州湾表层沉积物中多环芳烃的分布及来源[J].海洋环境科学,2003,22(4):38-43.
[28]田蕴,郑天凌,王新红.厦门西港表层沉积物中多环芳烃(PAHs)的含量、分布及来源[J].海洋与湖沼,2004,35(1):15-20.
[29]麦碧娴,林峥,张干,等.珠江三角洲河流和珠江口表层沉积物中有机污染物研究-多环芳烃和有机氯农药的分布特征[J].环境科学学报,2000,20(2):192-197.
[30]Yan L,Pui K F,Man F T,et al.Sources and seasonal variation of PAHs in the sediments of drinking water reservoirs in Hong Kong and the Dongjiang River(China)[J].Environ Monit Assess,2002,133:447-458.
[31]江锦花,丁理法.台州湾五种海洋生物体内多环芳烃的浓度、富集特征及环境效应[J].环境污染与防治,2007,29(5):394-397.
[32]Kan H,Leinemann M.Determination of polycyclic aromatic hydrocarbons in smoked fisherf products from different smoking kilns[J].Z.Lebensm.Unters Forsh,1996,202(6):458-464.
[33]Madhavan N D,Naidu K A.Polycyclic aromatic hydrocarbons in placenta,maternal blood,umbilical card blood and milk of Indian women[J].Hum.Exp.Toxicol,1995,14(6):503-506.
[34]Juhasz A L,Meghara J M,Naidu R.Bioavailability:the major challenge(constraint)to bioremediation of organically contaminated soils[A].In:WISE D L,et al.Biore-mediatiom of Contaminated Soils[C].New York:Marcel Dekker Inc,2000:217-241.
[35]Chung N,Alexander M.Effect of soil properties on bioavailability and extractability of phenanthrene and atrazine sequestered in soil[J].Chemosphere,2002,48:109-115.
[36]Rittmann B E,Mccarty P L.Environmental biotechnology:principles and applications [M].New York:McGraw-Hill Com Inc,2001:695-730.
[37]Lu X X.Bioavailability and bioaccumulation of sediment-associated,desorption-resistant fraction of polycyclic aromatic hydrocarbon contaminants[D].USA:Louisiana State University and Agricultural and Mechanical College,2003.
[38]Richard G,Luthy,Richelle M,et al.Bioavailability of contaminants in soils and sediments[M],2002.
[39]Reid B J,Jones H C.Bioavailability of persistent organic pollutants in soils and sediments-a perspective on mechanisms,consequences and assessment[J].Environ Pollution,2000,108:103-112.
[40]Hatzinger P B,Alexander M.Effect of ageing of chemicals in soil on their biodegradability and extractability[J].Environ Sci Technol,1995,29:537-545.
[41]Theng B K,Aislabie J,Fraser R.Bioavailability of phenanthrene intercalated into analkylammonium-montmorillonite clay[J].Soil Biol Biochem,2001,33:845-848.
[42]Tang J,Liste H,Alexander M.Chemical assays of availability to earthworms of polycyclic aromatic hydrocarbons in soil[J].Cheosphere,2002,48:35-42.
[43]Johnson D L,Jones K C,Langdon C J,et al.Temporal changes in earthworm availability and extractability of polycyclic aromatic hydrocarbons in soil[J].Soil Biology and Biochemistry,2002,34:1363-1370.
[44]Lu X X,Danny D,Beible,et al.Biovailability of desorption-resistant Phenanthrene to the Oligochaete Ilyodrilus ternpletoni[J].Environmental Toxicology and Chemistry,2003,22(1):153-160.
[45]陶玉强,张淑贞,许宜平,等.利用三油酸甘油酯-醋酸纤维素复合膜评价土壤中多环芳烃的生物可利用性[J].生态毒理学报,2007,2(3):284-291.
[46]Pignatello J J,Xing B.Mechanisms of slow sorption of organic chemicals to natural particles[J].Environmental Science and Technology,1996,30,1-10.
[47]Beckles,D M.Bioavailability of polycyclic aromatic hydrocarbons as sediment associated desorption-resistant contaminants[D].Houston:Rice University,2000.
[48]Karickhoff S W,Brown D S,Scott T A.Sorption of hydrophobic pollutants on natural sediment[J].Water Research,1979,13,241-248.
[49]Karickhoff S W.Semi-empirical estimation of sorption of hydrophobic pollutants on natural sediments and soils[J].Chemosphere,1981,10,833-846.
[50]Means J C,Wood S G,Hassett J J,et al.Sorption of polynuclear aromatic hydrocarbons by sediments and soils[J].Environmental Science and Technology,1980,14,1524-1528.
[51]Pereira,W E,Rostad,C E,Chiou,C T,et al.Contamination of estuarine water,biota,and sediment by helogenated organic compounds:a field study[J].Environmental Science and Technology,1988,27:772-778.
[52]Readman,J W,Mantoura,R F,Rhead,M M.A record of polycyclic aromatic hydrocarbons(PAH) pollution obtained from accreting sediments of the Tamar estuary,U K:evidence for non-equilibrium behavior of PAH[J].Science of Total Environmeny,1987,66:73-94.
[53]Chen,W,Kan,A T,Fu G,et ai.Adsorption-desorption behaviors ofhydrophobic organic compounds in sediments of lake Charles,Louisiana,USA[J].Environmental Toxicology and Chemistry,1999,18,1610-1616.
[54]Kan A T,Fu G,Hunter M,et al.Irreversible adsorption of neutral organic hydrocarbons-Experimental observations and modelpredictions[J].Environmental Science and Technology,1998,32:892-902.
[55]Chen W,Kan A T,Tomson M B.Irreversible adsorption of chlorinated benzenes to natural sediments-implication for sediment quality criteria[J].Environ Sci Technol,2000,34:385-392.
[56]Landrum P F,Robbins J A.Bioavailability of sediment-associated contaminants to benthic invertebrates[J].Sediments:Chemistry and Toxicity of In-Place Pollutants,1990,23:237-263.
[57]黄建东,洪华生,等.厦门大学海滨沙滩动力沉积环境特征[J].福建环境,1999,16(3):9-11.
[58]刘宪斌,朱琳,张福,等.天津塘沽驴驹河-独流减河潮间带沉积物特征研究[J].海洋通报,2005,24(3):37-41.
[59]张青田,孟迪,胡桂坤.由渤海海岸带生境修复谈泥螺[J].海洋信息,2006,(1):12-13.
[60]顾晓英,尤仲杰,王一农,等.泥螺Bullacta exarata生长生物学的初步研究[J].浙江水产学院学报,1997,16(1):6-11.
[61]李树国.泥螺的生物学[J].水利渔业,2005,25(4):42-43.
[62]Edgren M.Sediment as indicator of pollution[J].Ambio,1977,5,133-146.
[63]马德毅.海洋沉积物的污染指示作用和检测方法[J].海洋通报,1993,12(5):89-96.
[64]袁兴中.河口潮滩湿地底栖动物群落的生态学研究[D].上海:华东师范大学,2001.
[65]GB/T17378.3-1998,海洋监测规范第3部分:样品采集、贮存与运输[S].北京:海洋出版社,1998.
[66]朱文锋.广东省沿海表层沉积物中多环芳烃研究[D].广东:中国科学院广州地球化学研究所,2007.
[67]GB/T 17378.5-1998,海洋监测规范:沉积物分析[S].北京:国家海洋局,1998.
[68]GB/T13909-1992,海洋调查规范:海洋地质地球物理调查方法[S].北京:国家技术监督局,1992.
[69]Dong R B,Xu D F,Liu L,et al.Behavior ofpolynuclear aromatic hydrocarbons in Environment[J].Environment and Development,1999,14(4):10-12.
[70]吴起航,麦碧娴,杨清书,等.沉积物中多环芳烃和有机氯农药赋存状态[J].中国环境科学,2004,24(1):89-93.
[71]张宗雁,郭志刚,张干,等.东海泥质区表层沉积物中多环芳烃的分布特征及物源[J].地球化学,2005,34(4):379-386.
[72]陈卓敏,高效江,宋祖光,等.杭州湾潮滩表层沉积物中多环芳烃的分布及来源[J].中国环境科学,2006,26(2):233-237.
[73]党志,于虹,黄伟林,等.土壤/沉积物吸附有机污染物机理研究的进展[J].化学通报,2001,2:81-85.
[74]Master D J,MeKee B A,Nittrouer C A,et al.Rates of sediments accumulation and particle reworking based on radiochemical measurements from continental shelf deposits in the East China Sea[J].Continental ShelfRes,1985,4(1-2):143-158.
[75]Christensen M,Andersen O,Banta G T.Metabolism of pyrene by the polychaetes Nereis diversieolor and Arenicola marina[J].AquaToxicol,2002,58:15-25.
[76]Fernandes M B,Sicre M A,Boireau A,et al.Polyaromatic hydrocarbon (PAH)distributions in the Scine River and its estuary[J].Marine Pollution Bulletin,1999,38(1):7-15.
[77]Dickhut R M,Canuel E A,Gustafson K E,et aI.Automatic sources of carcinogenic polycyclic aromatic hydrocarbons associated with particulate matter in the Chesapeake Bay region[J].Environ Sci Technol,2000,34(21):4635-4640.
[78]Witt G.Polycyclic aromatic hydrocarbons in water and sediment of The Baltic Sea[J].Mar Pollut Bull,1995,31(4):237-248.
[79]Long E R,Macdonald D D,Smith S L et al.Incidence of adverse biological effects with ranges of chemical concentrations in marine and estuarine sediments [J].Environmental Management,1995,19(1):81-97.
[80]Durou C,Mouneyrac C,Amiard-Triquet C.Tolerance to metals and assessment of energy reserves in the polychaete Nereis diversicolor in clean and contamined estuaries[J].Environ Toxicol,2005,20:23-31.
[81]Warnau M R,Biondo A,Temara J M,et al.Distribution of heavy metals in the echinoid Paracentrotus lividus from the Mediterranean Posidonia oceanica ecosystem:seasonal and geographical variations[J].Sea Res.,1998,39:267-280.
[82]李丽娜,陈振楼,许世远,等.长江口滨岸潮滩底栖动物泥螺受铜污染的毒理学研究[J].海洋环境科学,2004,23(3):24-27.
[83]李丽娜,陈振楼,许世远,等.长江口滨岸潮滩底栖泥螺受铅污染的急性毒理试验[J].海洋湖沼通报,2005,7(2):88-92.
[84]戴树桂.环境化学[M].北京:高等教育出版社,1985,161-163.
[85]顾晓英,尤仲杰,王一农,等.泥螺Bullacta exarata生长生物学的初步研究[J].浙江水产学院学报,1997,16(1):6-11.
[86]李树国.泥螺的生物学[J].水利渔业,2005,25(4):42-43.
[87]王一农,尤仲杰,於宏,等.养殖泥螺生态习性研究[J].宁波大学学报(理工版),2003,16(3):241-245.
[88]湖南医学院卫生学教研组.卫生毒理实验方法[M].人民卫生出版社,1979,4.
[89]赵作媛.镉-菲复合污染对蚯蚓的急性毒性效应及抗氧化酶的影响[D].上海:上海交通大学,2007.
[90]苗晶晶.多环芳烃化合物对栉孔扇贝致毒机理的研究[D].山东:中国海洋大学,2006.
[91]Lake J L,Rubinstein N I,Pavignano S.Predicting bioaccumulation:Development of a partitioning model for use as a screening tool in regulating ocean disposal of wastes.In Dickson KL,Brungs WA,eds,Fate and effects of sediment-bound chemicals in aquatic systems[M].Pergamon press,New York,USA,1987,151-166.
[92]Ankley G T,Call D J,Hoke R A,et al.Bioaccumulation of PCBs from sediments by Oligochaetes and fishes:Comparison of laboratory and field studies[J].Canadian Journal of Fish Aquatic Science,1992,49:2080-2085.
[93]Millward R N,Fleeger J W,Reible D D.Pyrene bioaccumulation,effects of pyrene exposure on particle-size selection,and fecal pyrene content in the Oligochaete Limnodrilus hoffmeisteri(Tubificide,Oligochaete)[J].Environmental Toxicology and Chemistry,2001,20:1359-1366.
[94]Kraaij R,Mayer P,Busser F J,et al.Measured pore water concentrations make equilibrium portioning work-a data analysis[J].Environmental Science and Technology,2003,37:268-274.
[95]陈华林,陈英旭,王子健,等.中国南方河流和湖泊沉积物对菲的吸附特性[J].环境科学,2003,24(5):120-124.
[96]Alexander M.Aging,bioavailability,and overestimation of risk from environmental pollutants[J].Environ Sci Technol,2002,34(20):4259-4265.
[97]SanderM,Lu Y,Pignatello J J.Conditioning-annealing studies of natural organic matter solids linking irreversible sorption to irreversible structural expansion [J].Environ Sci Technol,2006,40(1):170-178.
[98]罗晓丽,齐亚超,张承东,等.多环芳烃在中国两种典型土壤中的吸附和解吸行为研究[J].环境科学学报,2008,28(7):1375-1380.