自制多器官保存液的实验研究
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摘要
目的研制具有自主知识产权的多器官保存液并验证其保存效果。方法在既往长征医院器官保存液研制的基础上研制出SMO多器官保存液。通过SMO液对小型猪肾脏低温保存过程中形态学改变和代谢研究评价其对肾脏保存效果;用SMO液低温保存小型猪肝脏12小时后原位肝移植评价其对肝脏保存效果。结果SMO液在短时间内低温保存肾脏效果与HCA-II液相同,对肾脏长时间低温保存的效果则好于HCA-II液。SMO液能够维持肝脏组织完整的功能形态,有效保护肝脏微循环,减轻低温保存损伤和再灌注损伤,保证了供肝具有良好活力。结论SMO多器官保存液缓冲能力强大,能量底物充足,既维持溶液的高渗特性又避免了粘滞度过高,保持中等浓度的钾离子和钠离子减轻了细胞水肿和高钾的危害,同时加入了中药的有效活性成分,具有鲜明的中国特色。
Objectives To develop a new multiple organ preservation solution with independent intellectual property rights and evaluate the effect of the new preservation solution on organ storage. Methods The new multiple organ preservation solution-SMO solution was developed on the base of organ preservation solutions developed by Changzheng Hospital before. The effect of SMO solution on organ preservation was evaluated through the isolated miniature pig kidney cold storage model and miniature pig orthotopic liver transplantation model compared with HCA-II solution. Results SMO solution was better than HCA-II solution in long time kidney cold storage. SMO solution can maintain the hepatic functional form and protect liver microcirculation to attenuate cold injury and reperfusion injury. Conclusions The SMO solution possessed powerful buffer capacity and offered sufficient energy substrates. It maintained the high osmotic character and avoided the hyperviscosity and attenuated the cellular edema and the harm of high kalium ion concentration. At the same time, the effective traditional Chinese medicine active component was added into the SMO solution which possessed Chinese characteristic.
Objectives To develop a new multiple organ preservation solution with independent intellectual property rights and evaluate the effect of the new preservation solution on organ storage. Methods The new multiple organ preservation solution-SMO solution was developed on the base of organ preservation solutions developed by Changzheng Hospital before. The effect of SMO solution on organ preservation was evaluated through the isolated miniature pig kidney cold storage model and miniature pig orthotopic liver transplantation model compared with HCA-II solution. Results SMO solution was better than HCA-II solution in long time kidney cold storage. SMO solution can maintain the hepatic functional form and protect liver microcirculation to attenuate cold injury and reperfusion injury. Conclusions The SMO solution possessed powerful buffer capacity and offered sufficient energy substrates. It maintained the high osmotic character and avoided the hyperviscosity and attenuated the cellular edema and the harm of high kalium ion concentration. At the same time, the effective traditional Chinese medicine active component was added into the SMO solution which possessed Chinese characteristic.
引文
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