生物吸附法去除电镀废水中镉的研究
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摘要
重金属污染是危害最大的环境污染问题之一。镉是毒性较大的重金属,环保法规定其为一类污染物。电镀是当今全球三大污染工业之一,一般电镀厂的含镉废水在处理前镉的浓度都远高于国家制定的排放标准。因而,电镀废水中镉的去除尤为重要。利用微生物吸附镉等重金属离子具有许多优点,例如：吸附速度快；在低浓度下,金属可以被选择性去除；处理效率高；pH值和温度条件范围宽；投资少,运行费用低；可有效地回收一些贵重金属等。近年来,对于采用生物吸附法去除废水中重金属离子,国内外专家学者开展了较为广泛的研究。
本研究以废啤酒酵母、沟戈登氏菌、胶质芽胞杆菌和枯草芽胞杆菌为吸附剂,以电镀厂含镉电镀废水为处理对象,采用沉淀法或浮选法实现固液分离,达到除镉的目的。
本文研究了微生物形态与性质,对废啤酒酵母、沟戈登氏菌、胶质芽孢杆菌及枯草芽胞杆菌的染色特征和扫描电子显微镜形貌以及透射电子显微镜结构进行了观察,测试了它们的荷电特性、细胞壁组成及特征官能团。研究发现,它们各自的表面化学特性对其吸附过程产生不同的影响。本文研究了电镀废水的相关物理化学性质及主要成分的存在状态；研究了废水pH值、吸附剂用量、吸附时间、吸附温度、搅拌速度电镀废水中镉吸附效果的影响；研究了废啤酒酵母水洗前后不同沉降时间的沉淀效果；研究了以沟戈登氏菌、胶质芽孢杆菌及枯草芽胞杆菌为吸附剂时,不同pH值及捕收剂用量对微生物吸附-浮选除镉效果的影响。依据动电电位、微生物表面荷电状态、红外光谱、扫描电子显微镜、光电子能谱等测试结果,探索了微生物吸附剂的作用机理。
研究结果表明,4种吸附剂对该电镀废水中镉均具有较好的吸附效果。废啤酒酵母经简单水洗处理后,在常规条件下,对电镀废水中镉的吸附率为96.18%,其它三种吸附剂对电镀废水中镉的吸附率均在92%以上。研究所用废啤酒酵母具有较好的沉淀性能,采用吸附-沉淀法去除电镀废水的镉,能获得较好的吸附及固液分离指标。在电镀废水中镉浓度约为26mg/L、水洗废啤酒酵母菌用量为11.4g/L、pH为7、室温(约18℃)、生物吸附时间为30min的条件下,自然沉降3.5h,镉去除率达96%以上。以沟戈登氏菌、胶质芽孢杆菌及枯草芽胞杆菌为吸附剂时,采用浮选法进行固液分离,在电镀废水中镉浓度约为26mg/L、枯草芽胞杆菌用量为10g/L(湿重)、pH为7、室温(约25℃)、生物吸附时间为10min的条件下,以二正丁胺为捕收剂,在用量为4×10-4mol/L时,镉去除率达到76%。以水洗废啤酒酵母为吸附剂,采用吸附-沉淀法对该电镀废水中镉的去除效果明显好于以沟戈登氏菌、胶质芽孢杆菌及枯草芽胞杆菌为吸附剂,采用吸附-浮选法,对该电镀废水中镉的去除效果。加之,废啤酒酵母菌来源广、价格低,比其它三种生物吸附剂具有更大的优势。
采用自制装置,以水洗废啤酒酵母为吸附剂,采用吸附-沉淀法进行了扩大试验研究,在电镀废水中镉浓度为26mg/L左右、水洗废啤酒酵母菌用量为40g/L(湿重)、pH为7、室温(约25℃)、生物吸附时间为30min的条件下,自然沉降4h,镉去除率达94%以上；进行的小型连续试验的结果表明,当废水给入速度为10.8mL/min、40mL/min、167mL/min时,水洗废啤酒酵母对电镀废水中镉的吸附率分别为98.43%、96.46%和86.86%。
Heavy metals pollution is the one of the biggest environment pollution problems. Cadmium is the most poisonous heavy metal,the enviroment protection law stipulates that cadmium is the first kind of pollution materials.Electroplating is one of three big pollution industry,in general the Cadmium concentration of the Cadmium-containing electroplating wastewater doesn't meet domestic discharge standard before treatment,so removal of Cadmium in electroplating wastewater is very important. Removing heavy metals by biosorption has many advantages,such as,fast speed, heavy metal can be removed selectively even at its low concentration,high efficiency, a wide range of pH and temperature,less investment and running cost,in addition,some heavy metals can be recovered. Many experts domestic and abroad do a lot of work on biosorption of heavy metals for several years.
Waste Saccharomyces cerevisiae,Gordona amarae,Bacillus mucilayinosus and Bacillus subtilis was used as biosorbent,Cadmium-containing electroplating wastewater of an electroplating plant was used to treat objects in studies,separation of solid and liquid was done by precipitation and flotation methods to remove cadmium from wastewater.
The shape and characters of micro biology was studied and stain characters,shape appearance and structure of waste saccharomyces cerevisiae,Gordona amarae,Bacillus mucilayinosus and Bacillus subtilis were observed by biology microscope,SEM and TEM, their charge characters and the composition of cell wall and function groups were tested. The research results showed their chemistry characters had different effect on biosorption process, the physics and chemistry naturals and state of main component were studied in the paper.Influence of pH value,dosage of adsorbent,the temperature,the stiring revolution and the contact time on adsorption result were studied. The effect on precipitation result of different precipitation time of waste Saccharomyces cerevisiae before and after water-washing,flotation and removing cadmium influence of different pH value and dasoge of collectors using Gordona amarae,Bacillus mucilayinosus and Bacillus subtilis as adsorbent were studied. The adsorption mechanism to cadmium wasdiscussed by test results of Zeta potential,charge state of cells surface,infrared spectroscopy analysis of the cells, SEM,TEM and XPS
The research results show that adsorption influence of four adsorbents to cadmium in electroplating wastewater are good. The adsorption rate of cadmium in electroplating wastewater is above 96.18% by water-wsahing waste Saccharomyces cerevisiae on the normal condition,adsorption rate of cadmium are all above 92% by the other three adsorbents. Precipitation performance of waste Saccharomyces cerevisiae used in studies is good,the good index of adsorption and solid-liquid separation are got by adsorption-precipitation method by waste Saccharomyces cerevisiae,removal rate of cadmium is over 96% after adsorbing for 30 min with cadmium concentration in cadmium-containing electroplating wastewater about 26mg/L,waste Saccharomyces cerevisiae 11.4 g/L,temperature 18℃,pH 7.0 and static precipitation for 3.5 hours. The flotation method is used in solid-liquid separation when Gordona amarae,Bacillus mucilayinosus and Bacillus subtilis are used adsorbents,removal rate of cadmium was over 76% after adsorbing for 10 min with cadmium concentration in cadmium-containing electroplating wastewater about 26mg/L, Bacillus subtilis 10 g/L(wet weight),at room temperature(about 18℃),pH 6.0 and flotating by collector dibutyamine 4×10-4mol/L for 4min. The effct of removal cadmium adsorption-precipitation by waste Saccharomyces cerevisiae is better than the index of adsorption-flotation by Gordona amarae,Bacillus mucilayinosus and Bacillus subtilis obviously. Additionally, because of abundant waste Saccharomyces cerevisiae and low price,it has more obvious advantages compared with the other three biosorbents.
The wide experiments are done with self-made equipment,and the good removal cadmium result is got using adsorption-precipitation by water-wsahing waste saccharomyces cerevisiae,removal rate of cadmium is over 94% after adsorbing for 30 min with cadmium concentration in cadmium-containing electroplating wastewater about 26mg/L,waste saccharomyces cerevisiae 40 g/L(wet weight),at room temperature(about 25℃),pH 7.0 and static precipitation for 4 hours. The continuing adsorption experiments are done with self-made equipment,and the adsorption rate of cadmium is 98.43%,96.46% and 86.86% repectively when the velocity of inflow of wastewater is 10.8 mL/min,40 mL/min and 167mL/min.
本研究以废啤酒酵母、沟戈登氏菌、胶质芽胞杆菌和枯草芽胞杆菌为吸附剂,以电镀厂含镉电镀废水为处理对象,采用沉淀法或浮选法实现固液分离,达到除镉的目的。
本文研究了微生物形态与性质,对废啤酒酵母、沟戈登氏菌、胶质芽孢杆菌及枯草芽胞杆菌的染色特征和扫描电子显微镜形貌以及透射电子显微镜结构进行了观察,测试了它们的荷电特性、细胞壁组成及特征官能团。研究发现,它们各自的表面化学特性对其吸附过程产生不同的影响。本文研究了电镀废水的相关物理化学性质及主要成分的存在状态；研究了废水pH值、吸附剂用量、吸附时间、吸附温度、搅拌速度电镀废水中镉吸附效果的影响；研究了废啤酒酵母水洗前后不同沉降时间的沉淀效果；研究了以沟戈登氏菌、胶质芽孢杆菌及枯草芽胞杆菌为吸附剂时,不同pH值及捕收剂用量对微生物吸附-浮选除镉效果的影响。依据动电电位、微生物表面荷电状态、红外光谱、扫描电子显微镜、光电子能谱等测试结果,探索了微生物吸附剂的作用机理。
研究结果表明,4种吸附剂对该电镀废水中镉均具有较好的吸附效果。废啤酒酵母经简单水洗处理后,在常规条件下,对电镀废水中镉的吸附率为96.18%,其它三种吸附剂对电镀废水中镉的吸附率均在92%以上。研究所用废啤酒酵母具有较好的沉淀性能,采用吸附-沉淀法去除电镀废水的镉,能获得较好的吸附及固液分离指标。在电镀废水中镉浓度约为26mg/L、水洗废啤酒酵母菌用量为11.4g/L、pH为7、室温(约18℃)、生物吸附时间为30min的条件下,自然沉降3.5h,镉去除率达96%以上。以沟戈登氏菌、胶质芽孢杆菌及枯草芽胞杆菌为吸附剂时,采用浮选法进行固液分离,在电镀废水中镉浓度约为26mg/L、枯草芽胞杆菌用量为10g/L(湿重)、pH为7、室温(约25℃)、生物吸附时间为10min的条件下,以二正丁胺为捕收剂,在用量为4×10-4mol/L时,镉去除率达到76%。以水洗废啤酒酵母为吸附剂,采用吸附-沉淀法对该电镀废水中镉的去除效果明显好于以沟戈登氏菌、胶质芽孢杆菌及枯草芽胞杆菌为吸附剂,采用吸附-浮选法,对该电镀废水中镉的去除效果。加之,废啤酒酵母菌来源广、价格低,比其它三种生物吸附剂具有更大的优势。
采用自制装置,以水洗废啤酒酵母为吸附剂,采用吸附-沉淀法进行了扩大试验研究,在电镀废水中镉浓度为26mg/L左右、水洗废啤酒酵母菌用量为40g/L(湿重)、pH为7、室温(约25℃)、生物吸附时间为30min的条件下,自然沉降4h,镉去除率达94%以上；进行的小型连续试验的结果表明,当废水给入速度为10.8mL/min、40mL/min、167mL/min时,水洗废啤酒酵母对电镀废水中镉的吸附率分别为98.43%、96.46%和86.86%。
Heavy metals pollution is the one of the biggest environment pollution problems. Cadmium is the most poisonous heavy metal,the enviroment protection law stipulates that cadmium is the first kind of pollution materials.Electroplating is one of three big pollution industry,in general the Cadmium concentration of the Cadmium-containing electroplating wastewater doesn't meet domestic discharge standard before treatment,so removal of Cadmium in electroplating wastewater is very important. Removing heavy metals by biosorption has many advantages,such as,fast speed, heavy metal can be removed selectively even at its low concentration,high efficiency, a wide range of pH and temperature,less investment and running cost,in addition,some heavy metals can be recovered. Many experts domestic and abroad do a lot of work on biosorption of heavy metals for several years.
Waste Saccharomyces cerevisiae,Gordona amarae,Bacillus mucilayinosus and Bacillus subtilis was used as biosorbent,Cadmium-containing electroplating wastewater of an electroplating plant was used to treat objects in studies,separation of solid and liquid was done by precipitation and flotation methods to remove cadmium from wastewater.
The shape and characters of micro biology was studied and stain characters,shape appearance and structure of waste saccharomyces cerevisiae,Gordona amarae,Bacillus mucilayinosus and Bacillus subtilis were observed by biology microscope,SEM and TEM, their charge characters and the composition of cell wall and function groups were tested. The research results showed their chemistry characters had different effect on biosorption process, the physics and chemistry naturals and state of main component were studied in the paper.Influence of pH value,dosage of adsorbent,the temperature,the stiring revolution and the contact time on adsorption result were studied. The effect on precipitation result of different precipitation time of waste Saccharomyces cerevisiae before and after water-washing,flotation and removing cadmium influence of different pH value and dasoge of collectors using Gordona amarae,Bacillus mucilayinosus and Bacillus subtilis as adsorbent were studied. The adsorption mechanism to cadmium wasdiscussed by test results of Zeta potential,charge state of cells surface,infrared spectroscopy analysis of the cells, SEM,TEM and XPS
The research results show that adsorption influence of four adsorbents to cadmium in electroplating wastewater are good. The adsorption rate of cadmium in electroplating wastewater is above 96.18% by water-wsahing waste Saccharomyces cerevisiae on the normal condition,adsorption rate of cadmium are all above 92% by the other three adsorbents. Precipitation performance of waste Saccharomyces cerevisiae used in studies is good,the good index of adsorption and solid-liquid separation are got by adsorption-precipitation method by waste Saccharomyces cerevisiae,removal rate of cadmium is over 96% after adsorbing for 30 min with cadmium concentration in cadmium-containing electroplating wastewater about 26mg/L,waste Saccharomyces cerevisiae 11.4 g/L,temperature 18℃,pH 7.0 and static precipitation for 3.5 hours. The flotation method is used in solid-liquid separation when Gordona amarae,Bacillus mucilayinosus and Bacillus subtilis are used adsorbents,removal rate of cadmium was over 76% after adsorbing for 10 min with cadmium concentration in cadmium-containing electroplating wastewater about 26mg/L, Bacillus subtilis 10 g/L(wet weight),at room temperature(about 18℃),pH 6.0 and flotating by collector dibutyamine 4×10-4mol/L for 4min. The effct of removal cadmium adsorption-precipitation by waste Saccharomyces cerevisiae is better than the index of adsorption-flotation by Gordona amarae,Bacillus mucilayinosus and Bacillus subtilis obviously. Additionally, because of abundant waste Saccharomyces cerevisiae and low price,it has more obvious advantages compared with the other three biosorbents.
The wide experiments are done with self-made equipment,and the good removal cadmium result is got using adsorption-precipitation by water-wsahing waste saccharomyces cerevisiae,removal rate of cadmium is over 94% after adsorbing for 30 min with cadmium concentration in cadmium-containing electroplating wastewater about 26mg/L,waste saccharomyces cerevisiae 40 g/L(wet weight),at room temperature(about 25℃),pH 7.0 and static precipitation for 4 hours. The continuing adsorption experiments are done with self-made equipment,and the adsorption rate of cadmium is 98.43%,96.46% and 86.86% repectively when the velocity of inflow of wastewater is 10.8 mL/min,40 mL/min and 167mL/min.
引文
1.三废治理与利用编委会.三废治理与利用[M].北京：冶金工业出版社,1995.
2.陈晓风.中国环境在呻吟[J].中国社会导刊,2006.
3.K.Chandra Sekha,N.S.Chary.Fractionation studies andbioaccumulation of sediment-bound heavy metals in Kollerulake by edible fish[J].Environmental International,2003 (29):1001-1008.
4.Aleya Begum,Md.Nurul Amin.Selected elemental composi-ton ofthemuscle tissue of three species of fish,Tilapia niltica,Cirrhina mrigala and Clarius batrachus from the freshwater Dhanmondi Lake in Bangladesh[J].Food Chemistry.2005 (93):439-443.
5.陈佩林.微生物吸附重金属离子研究进展[J].生物学教学,2003,28(12)：1-3.
6.陈勇生,孙启俊,陈钧,等.重金属的生物吸附技术研究[J],环境科学进展,1997,5(6)：34-43.
7.汪大,徐新华,宋爽.工业废水中专项污染物处理手册[M].北京：化学工业出版社,2000.61-61.
8.梅光良.重金属废水的危害及治理[J].微量元素与健康研究,2004,21(4)：54-56.
9.冯德福.镉污染与防制[J].沈阳化工,2000,29(1)：44-46.
10.中国认证人员国家注册委员会编.ISO14000环境管理体系国家注册审核员基础知识通用教程[M].北京：中国计量出版社,2000.
11.王璞,闵小波,柴立元.含镉废水处理现状及其生物处理技术的进展[J].工业安全与环保,2006,32(8)：14-16.
12.邱廷省,成先雄,郝志伟,等.含镉废水处理技术现状及发展[J].四川有色金属,2002,(4)：38-41.
13.姜述芹,周保学.氢氧化镁处理含镉废水的研究[J].环境化学,2003,22(6)：601-604.
14.陈晋阳,黄卫.无定形氢氧化铁吸附水溶液中镉离子机理的XPS研究[J]2002,21(3)：70-72.
15.马艳飞,王九思,宋光顺,等.氢氧化镁对废水重镉吸附性能的研究[J].兰州铁道学院学报(自然科学版),2003,22(4)：120-122.
16.张玉梅.含镉废水处理的试验研究[J].环境工程,1995,13(1)：15-20,21.
17.陆维国.铁氧体法处理电镀废水的应用研究[J].贵州环保科技,2005,11,(2)：32-35.
18.孙英,杨健男,方云如.铁氧体法处理含铬和镉废水及其含量测定[J].实验技术与管理,2000,17(4)：75-79.
19.张德强,康海彦,杨莉丽,等.离子交换树脂吸附Cd(Ⅱ)和Pb(Ⅱ)的研究[J].环境科学与技术,2003,S1：4-5,23.
20.杨莉丽,康海彦,李娜,等.离子交换树脂吸附镉的动力学研究[J].离子交换与吸附,2004,20(2)：138-143.
21.王代芝,黄育刚.酸改性膨润土处理含镉(Ⅱ)废水[J].无机盐工业,2005,37(2)：38-40.
22.杨胜科,费晓华.海泡石处理含镉废水技术研究[J].化工矿物与加工,2004,33(9)：16-17,20.
23.邓娟利,胡小玲,管萍,等.膜分离技术及其在重金属废水处理中的应用[J].材料导报,2005,19(2)：23-26.
24.王岩,王玉军,骆广生,等.中空纤维膜萃取镉离子的研究[J].化学工程,2002,30(5)：62-65.
25.胡厚堂,王海宁.生物吸附法处理水体中的重金属的现状与展望[J].新疆环境保护2003,25(4)：22-25.
26.Mohd Ikram Ansari,and Abdul Malik. Biosorption of nickel and cadmium by metal resistant bacterial isolates from agricultural soil irrigated with industrial wastewater[J].Bioresource Technology,2007,98(16):3149-3153.
27.邱廷省,成先雄.啤酒酵母吸附镉离子的试验研究[J].环境污染与防治,2004,26(2)：95-97.
28.Lodeiro P,Barriada J L,Herrero R,et al. The marine macroalga Cys-toseira baccata as biosorbent for cadmium(Ⅱ)and lead(Ⅱ)removal:Kinetic and equilibrium studies[J].Environmental Pollution,2005,15:110.
29.Hashim M A,Chu K H. Biosorption of cadmium by brown,green,and red seaweeds[J].Chemical Engineering Journal,2004,97:249-255.
30.叶锦韶,尹华,彭辉,等.重金属的生物吸附研究进展[J].城市环境与城市生态,2001,14(3)：30-32.
31.Chen J Z,Tao X C,Xu J,et al. Biosorption oflead, cadmium and mercury by immobilized Microcystis aeruginosa in acolumn[J].Process Biochemistry,2005,40(12):3675-3679.
32.Takata M,Matsuoka H,Ban H, et al. Biosynthesis of PHBV by mutant of Sphaerotilus natans [J],Appl. Microbiol. Biotech.,1995,44(2):37-42.
33.Saadet S, Osman G,Erman S,et al.Adsorption of Cd(Ⅱ),Cu(Ⅱ) and Ni(Ⅱ)ions by lemna minor L:Effect of physicochemical environment[J].Journal of Hazardous Materials,2005,126(1-2):96-104.
34.Christian V,Taty-costodes,Henri F,Catherine P,et al.Remaval of Cd(Ⅱ)and Pb(Ⅱ) ions fuo aqueous solution by adsorption onto sawdust of pinus sylvestris[J].Journal of Hazardous Materials,2003,105:121-142.
35.周东琴,魏德洲.草分枝杆菌及其吸附Pb2+后的可浮性研究[J].环境科学,2006,27(2)：338-342.
36.朱一民,苏秀娟,魏德洲,等.沉淀酵母菌对Pb(Ⅱ)的吸附机理研究[J].安全与环境学报,2006,6(6)：63-66.
37.苏秀娟,朱一民,沈岩柏,等.悬浮酵母菌对重金属Hg(Ⅱ)的吸附机理[J].安全与环境学报,2006,6(6)：63-66.
38.关晓辉,秦玉春,赵洁,等.浮游球衣菌去除废水中Pb2+的研究。环境科学,2005,26(6)：1107-1110.
39.P.Ligy, I.Leela, C.Venkobachar. Site of interaction of copper on Bacillus polymyxa[J].Water,Air and Soil Pollution,2000,119:11-21.
40.L.Asuncion, L.Naria, M. Susana et al. Nickel biosorption by free and immobilized cells of Pseudomonas fluorescens 4F39:A comparative study[J].Water, Air and Soil Pollution,2002,135:157-172.
41.N. Akira, Y. Masahide, Y.Hidekatsu et al. Copper biosorption by chemically treated Micrococcus Luteus cells[J].World Journal of Microbiology & Biotechnology,2001,7:343-347.
42.刘月英,傅锦坤,李仁忠,等.细菌吸附Pd2+的研究[J].微生物学报,2000,20(5)：535-539.
43.T. Suzuki, T. Hatsushika, A.Y. Hayakaw. Synathetic hydroxgapatite employed as inorganic cation exchangers[J].Journal of Chemical Society Faradary Transaction,1981,77:1059-1062.
44.E.R.El-Helos, S.Sabry,R.M. Amer. Cadmium biosorption by a cadium resistant strain of Bacillus thuringiensisi regulation and optimization of cell surface affinity for metaleation[J].Bio, Metals,2000,13:273-280.
45.陈明,赵永红.微生物吸附重金属离子的试验研究[J].南方冶金学院学报,2001,22(3)：168-173.
46.李明春,姜恒,候文强,等.酵母菌对重金属离子的吸附的研究.[J].菌物系统,1998,17(4).
47.Holan Z R, Volesky B. Biosorption of cadmidum by biomass of marine algae[J].Biotechnol. Bioeng, 1993,41:819-825.
48.Volesky B,Ciminelli VST.Assessment of interference in biosorption of heavy metals[J].Biotech.and Bioeng,1997,54(2):344-349.
49.牛慧,许学书,王建华.非生长产黄青霉菌吸附铅的研究[J].微生物学报,1993,33(6)：459-463.
50.李清彪,吴涓,杨宏泉,等.白腐真菌菌丝球形成的物化条件及其对铅的吸附[J].环境科学,1999,20(1)：33-38.
51.刘瑞霞,汤鸿霄,劳伟雄.重金属的生物吸附机理及吸附平衡模式研究[J].化学进展,2002,14(2)：87-92.
52.Nurgul B,Erdem K,Beyhan K.Biosorption of copper(Ⅱ) from aqueous solutions by wheat shell[J].Desalination,2004,164:135-140.
53.孟琴.生物吸附剂BAP对Cu2+的吸附研究[J].水处理技术,1998,24(3)：175-178.
54.吴乾青,李昕,李福德,等.微生物治理电镀废水的研究[J].环境科学,1997,9：47-50.
55.秦玉春,关晓辉,王立文,等.浮游球衣菌对Pb2+、Cu2+、Zn2+、d2+的吸附性能研究[J].环境污染与防治,2005,27(9)：648-650.
56.Tobin J M,Cooper D G.,Neufeld R J. Uptake of metal ions by Rhizopus arrhizus biomass[J],Appl. and Environ. Microbio.,1984,47(4):821-829.
57.Kuyucak N,Volesky B.Biosorbents for recovery of metals from industrial soluti ons[J],Biotechnol. Lett., 1988,10(2):137-142.
58.汤岳琴,林军,王建华.生物吸附研究进展[J],四川环境,2001,20(2)：12-16.
59.Muzzarelli R A A, Tubertini O. Chintin and chitosan as chromatographic supports and adsor-bents for collection of metal ions from organic and aqueous solutions and seawater[J].Talanta,1969,16:1571-1577.
60.Shumate ⅡS E,Strandberg G W.Accumulation of metals by microbial cells[J].New York:Pergamon Press Comprehensive Biotechnology,1985,4:235-239.
61.Ewa K, Francis A J. Immobilization of cadnium by microbial extracelluar products[J].Arch. Environ. Contam. Toxicol,1991,20:106-111.
62J.Yang, B. Volesky. Biosorption of uranium on sargassum biomass[J].Water Research,1999,33(15):3357-3363.
63.J.Wang. Biosorption of copper(Ⅱ)by chemically modified biomass of saccharomyces cerevisiae[J]. Process Biochemistry,2002,37(8):847-850.
64. Fourest, B. Volesky. Contribution of sulphonate guoups and alginate to heavymetal biosorption by the dry biomass of Sargassum fluitans[J].Environ Sci Technol,1996,30(1):277-282.
65.Y. Liu, J.K.Fu, R.Z. Li et al. Studies on biosorption of Pd2+ by bacteria[J].ActaMicrobio Sinn,2000,40(5):536-539.
66.lesky B.,May H.,Holan Z.R.,Biotech.and Bioeng.,1993,41,826.
67.ackwell K.J.,Singleton I.,Tobin J.M.,Appl.Microbiol.Biotechnol,1995,43,579.
68.黄民生,施华丽,郑乐平.曲霉对水中重金属的吸附去除[J].上海环境科学,2002,21(2)：89-92.
69.屠娟,张利,赵力,等.非活性根霉对废水中重金属离子的吸附[J].环境科学,1994,16(1)：12-15.
70.吴涓,李清彪,邓旭,等.白腐真菌吸附铅的研究[J].微生物学报,1999,39(1)：87-90.
71.周定.固定化细胞在废水处理中的应用及前景[J]环境科学,1998,14(5)：51-54.
72.A.M. Gaudin. Forth flotation-50th anniv[M].New York,Fuerstenau, D.W,1962,191.
73.A.I. Zouboulis, K.A. Matis, N.K. Lazaridis. Removal of metal ions from simulated wastewater by Saccharomyces yeast biomass:combining biosorption and flotation processes[J].Sep Sci Technol,2001,36:349-365.
74.K.A. Matis, G.P. Gallios, K.A. Kydros. Separation of fines by flotation techniques[J].Sep Technol,1993,3:76-90.
75.C.Alodrich, D.Feng. Removal of heavy metals from wastewater effluents by biosorptive flotation[J]. Minerals Engineering,13:1129-1138.
76.周东琴,魏德州.沟戈登氏菌对重金属的生物吸附-浮选和解吸性能[J].环境科学,2006,27(5)：961-964.
77.刘振扬,刘超.啤酒酵母在处理含镉工业废水中的应用研究[J].酿酒科技,2006(7)：85-88.
78.朱雪强,韩宝平.重金属生物吸附研究进展[J].中国环保产业,2004(5)：19-21.
79.Lodeiro P, Cordero B, Barriada J L, et al. Biosorption of cadmium bybiomass of brown marine macroalgae[J]. Journal of Hazardous Materials,2005,126(13):96-104.
80.陈国华.环境污染治理方法原理与工艺[M].北京：化学工业出版社,2003
81.Alena Luptakova, Maria Kusnierova. Bioremediation of acid minedrainage contaminated by SRB[J]. Hydrometallurgy,2005,77(1 2):97-102.
82.G Cabrera, RPerez, JMGomez, et al. Toxic effects of dissolved heavy metals on Desulfovibrio vulgaris and Desulfovibrio sp. strains[J].Journal of HazardousMaterials,2006,18:1-7.
83.D Fenga, CAldrich. Adsorption of heavy metals by biomaterials derived from the marine alga Ecklonia maxima[J].Hydrometallurgy,2004,73(12):1-10.
84.Pavel Kortrb,Lucie Doleckova,Vi ctor DeLorenzo,et al. Appl[J].Environ. Microbiol, 1999,65(3):1092-1098.
85.N Rangsayatorn, P Pokethitiyooka, E S Upatham, et al. Cadmiumbiosorption by cellsofSpirulina platensis TISTR8217 immobilized in alginate and silica gel[J].Environment International,2004,30(1):57-63.
86.MYakup Arlca, Gulay Bayramoglu, Meltem Yllmaz, et al. Biosorp-tion of Hg2+, Cd2+, and Zn2+ by ca alginate and immobilizedwoodrotting fungus Funalia trogii[J].Journal of HazardousMaterials,2004,109(1 3):191-199.
87.Kacar Y, Cigdem A, Sema T, et al. Biosorption of Hg(Ⅱ) and Cd(Ⅱ)from aqueous solutions: comparison of biosorptive capacity of alginate and immobilized live and heat inactivated Phanerochaete chrysosporium[J].Process Biochemistry,2002,37(6):601-610.
88.钱存柔,黄仪秀.微生物学试验教程[M].北京：北京大学出版社,2001.
89.辛宝平,陈桂淋,郑文钗.黑曲霉对弱酸性艳兰RAWL的吸附动力学和热力学研究[J].安全与环境学报,2006,6(5)：1-3.
90.马放,任南琪,杨基先.污染控制微生物学实验[M].哈尔滨：哈尔滨工业大学出版社,2002,6.
91.王金发,何炎明.细胞生物学实验教程[M].北京：科学出版社,2004,9.
92.Leo M/L.Nollet主编.袁洪福,褚小立,王艳斌,等译.水分析手册[M].北京：中国石化出版社,2005,6.
93.黄突.离子色谱仪的原理及应用[J].华中电力,2004,17(1)：69-70.
94.张建丽,刘志恒.诺卡氏型放线菌的分类[J].微生物学报,2001,4：513-517.
95.马邦亚,杨俊秀,曹支敏.放线菌分类研究的历史与现状[J].西北林学院学报,1999,14(3)：76-80.
96.徐成勇,袁野,黎丹辉,等.选择性分离放线菌[J].无锡轻工大学学报,1999,18(2)：45-49.
97.胥秀英,郑一敏,温寿祯,等.土壤放线菌分离方法的初步研究[J].生物学杂志,2000,17(2)：16.17.
98.杨宇容,徐丽华,李启任,等.放线菌分离方法的研究[J].微生物学通报,1995,22(2)：85-91.
99.吴襟,何秉旺.诺卡氏菌形放线菌β-甘露聚糖酶的纯化和性质[J].微生物学报,2000,40(1)：69-74.
100.H. Aimin, D.R. Simpson,L. Daniels et al. Cloning, expression, purification, and characterization of Nocardia sp. GTP cyclohydrolase I[J].Protein Expression & Purification,2004,35:171-180.
101.W. D. Kim, K. C. Daniel, J. Wang, et al. Heavy metal removal by activated sludge:influence of Nocardia amarae[J].Chemosphere,2002,46:137-142.
102.刘五星,徐旭士,杨启银,等.胶质芽孢杆菌发酵条件研究[J].南昌大学学报,2002,26(3)：299-302.
103.廖延雄,傅筱冲,蔡汝林,等.一株硅酸盐细菌的表型特征[J].江西科学,2000,18(3)：149-153.
104.王平宇,张树华.硅酸盐细菌的分离及生理生化特征的鉴定[J].南昌航空工业学院学报,2001,15(2)：78-82.
105.S.P. Vijayalakshmi, A.M. Raichur. Bioflocculation of high-ash India coals using Paenibacillus polymyxa[J].Mineral Processing,2002,67:199-210.
106. P.K. Sharma, K. Hanumantha Rao,K.S.E. Forssberg, K.A. Natarajan. Surface chemical characterisation of Paenibacillus polymyxa before and after adaptation to sulfide minerals[J].Mineral Processing,2001,62:3-25.
107.D. Santhiya, S. Subramanian, K.A. Natarajan. Surface chemical studies on sphalerite and galena using extracellular polysaccharides isolated from Bacillus polymyxa[J].Journal of Colloid and Interface Science,2002,256:237-248.
108.Namita Deo, K.A. Natarajan. Studies on interaction of Paenibacillus polymyxa with iron ore minerals in relation to beneficiation[J].Mineral Processing,1998,55:41-60.
109.王希成.生物化学[M].北京：清华大学出版社,2001.
110沈同.生物化学[M].北京：高等教育出版社,1999.
111.李建武.生物化学试验原理与方法[M].北京：北京大学出版社,1994.
112.B.R.格利克,J.J.帕斯捷尔纳克.分子生物技术[M].北京：化学工业出版社,2005.3.
113.D.W琼斯主编,江丕栋,等译.生物聚合物波谱学导论[M].北京：科学出版社,1983.
114.D.Lin-Vien.,N.B. Colthup.,W.G. Fateley et al. The handbook of infrared and raman characteristic frequencies of organie molecules[M].Academic Press,INC. San Diego. Canada,1991.
115.Van Loodrecht,M. C. M et al.The role of bacterial cell wall hydrophobicity in adhesion[J].Appl. Env. Microbiol,1987,53:1893-1897.
116.T.A. Stenstrom. Bacterial hydrophobicity, an overall parameter for the measurement of adhesion potential to soil particles[J].Appl. Env. Microbiol,1989,55:14-42.
117.G. P. Reid et al. Comparison of contact angles and adhesion to hexadecane of urogenital, dairy, and poultry lactobacillus:effect of serial culture passages[J].Appl. Env. Microbiol,1992,58:1549-1553.
118.N. Mozes,P.G. Rouxhet. Methods for measuring hydrophobicity of microorganism[J].Microbial. Methods,1987,6:99-112.
119.R.T. Neufeld et al. Cell surface measurements in hydrocarbon and carbonhydrate fermentations[J]. Appl. Env. Microbiol,1980,39:511-517.
120.方景礼.多元络合物电镀[M].国防工业出版社,1983,6.
121.汤岳琴,牛慧,林军,等.产黄青霉废菌体对铅的吸附机理研究[J],四川大学学报(工程科学版),2001,33(3)：50-54.
122.李志东,宋旭鹭,梁伟,等.啤酒酵母吸附重金属离子镉的研究[J].酿酒科技,2006,6：85-88.
123.刘刚,李清彪.重金属生物吸附的基础和过程研究[J].水处理技术,2002,28(1)：17-21.
124.马金鑫,朱国凯.扫描电子显微镜入门[M].北京：科学出版社,1985.
125.David J M,Alan J M,et al.The structure of a β-1,3-glucan from yeast cell walls[J].Biochem.J,1973B,135:11-18
126.朱淮武.有机分子结构波谱解析[M].北京：化工工业出版社,2005,9.
127.Bohn J A,Bemiller J N.(1→3)-β-glucans as biological response modifiers:a review of structure-funtional activity relationships[J].Carbohydrate Polymers,1995,28:13-14.
128.刘世宏,王当憨,潘成璜.X射线光电子能谱分析[M].北京：科学出版社,1988.
129.Zhong Y L,Jian M W,Ru X,et al.Spectroscopic characterization of Au3+ biosorption by waste biomass of saccharomyces cerevisiae[J].Spectrochimica Acta Part A,2005,61(6):761-765.
130.Pethkar A V,Kulkarni S K,Paknikar K M.Comparative studies on metal biosorption by two strains of cladosporium cladosporioides[J].Bioresource Technology,2001,80(3):211-215.
131.Dong H P,Yeoung S Y,Ji H J,et al.Mechanism of hexavalent chromium removal by dead fungal biomass of aspergillus niger[J].Water Research,2005,39(14):533-540.
132.Dao Z,Lina Z,Sheng L G.Mechanisms of lead biosorption on cellulose/chitin beads[J].Water Research,2005,39(16):3755-3762.
133.Subhashree P,Sarita S,Lal C R.Characterization of various functional groups present in the capsule of microcystis and study of their role in biosorption of Fe,Ni and Cr[J].Bioresource Technology,2007,98(3):595-601.
134.Shubo D,Yen-peng T. Characterization of PEI-modified biomass and biosorption of Cu(Ⅱ),Pb(Ⅱ) and Ni(Ⅱ)[J].Water Research,2005,39(10):2167-2177.
135.王恩德.环境资源中的微生物技术[M].北京：冶金工业出版社,1997.
136.有色金属冶金编辑委员会.中国冶金百科全书[M].北京：冶金工业出版社,1992.
137.汪贻水,王志雄,沈建忠.六十四种有色金属[M].长沙：中南工业大学出版社,1998.
138.四川大学化学信息中心.化学数据速查手册[M].化学工业出版社,华功电力出版中心出品,电子出版物数据中心.
139.Dudley H.Williams Ian Fleming著.王剑波,施卫峰译.有机化学中的光谱方法[M].北京：北京大学出版社2001,6.
140.岑沛霖,蔡谨.工业微生物学[M].北京：化学工业出版社,2000,6.
141.朱建光.浮选药剂[M].北京：冶金工业出版社,1993.
2.陈晓风.中国环境在呻吟[J].中国社会导刊,2006.
3.K.Chandra Sekha,N.S.Chary.Fractionation studies andbioaccumulation of sediment-bound heavy metals in Kollerulake by edible fish[J].Environmental International,2003 (29):1001-1008.
4.Aleya Begum,Md.Nurul Amin.Selected elemental composi-ton ofthemuscle tissue of three species of fish,Tilapia niltica,Cirrhina mrigala and Clarius batrachus from the freshwater Dhanmondi Lake in Bangladesh[J].Food Chemistry.2005 (93):439-443.
5.陈佩林.微生物吸附重金属离子研究进展[J].生物学教学,2003,28(12)：1-3.
6.陈勇生,孙启俊,陈钧,等.重金属的生物吸附技术研究[J],环境科学进展,1997,5(6)：34-43.
7.汪大,徐新华,宋爽.工业废水中专项污染物处理手册[M].北京：化学工业出版社,2000.61-61.
8.梅光良.重金属废水的危害及治理[J].微量元素与健康研究,2004,21(4)：54-56.
9.冯德福.镉污染与防制[J].沈阳化工,2000,29(1)：44-46.
10.中国认证人员国家注册委员会编.ISO14000环境管理体系国家注册审核员基础知识通用教程[M].北京：中国计量出版社,2000.
11.王璞,闵小波,柴立元.含镉废水处理现状及其生物处理技术的进展[J].工业安全与环保,2006,32(8)：14-16.
12.邱廷省,成先雄,郝志伟,等.含镉废水处理技术现状及发展[J].四川有色金属,2002,(4)：38-41.
13.姜述芹,周保学.氢氧化镁处理含镉废水的研究[J].环境化学,2003,22(6)：601-604.
14.陈晋阳,黄卫.无定形氢氧化铁吸附水溶液中镉离子机理的XPS研究[J]2002,21(3)：70-72.
15.马艳飞,王九思,宋光顺,等.氢氧化镁对废水重镉吸附性能的研究[J].兰州铁道学院学报(自然科学版),2003,22(4)：120-122.
16.张玉梅.含镉废水处理的试验研究[J].环境工程,1995,13(1)：15-20,21.
17.陆维国.铁氧体法处理电镀废水的应用研究[J].贵州环保科技,2005,11,(2)：32-35.
18.孙英,杨健男,方云如.铁氧体法处理含铬和镉废水及其含量测定[J].实验技术与管理,2000,17(4)：75-79.
19.张德强,康海彦,杨莉丽,等.离子交换树脂吸附Cd(Ⅱ)和Pb(Ⅱ)的研究[J].环境科学与技术,2003,S1：4-5,23.
20.杨莉丽,康海彦,李娜,等.离子交换树脂吸附镉的动力学研究[J].离子交换与吸附,2004,20(2)：138-143.
21.王代芝,黄育刚.酸改性膨润土处理含镉(Ⅱ)废水[J].无机盐工业,2005,37(2)：38-40.
22.杨胜科,费晓华.海泡石处理含镉废水技术研究[J].化工矿物与加工,2004,33(9)：16-17,20.
23.邓娟利,胡小玲,管萍,等.膜分离技术及其在重金属废水处理中的应用[J].材料导报,2005,19(2)：23-26.
24.王岩,王玉军,骆广生,等.中空纤维膜萃取镉离子的研究[J].化学工程,2002,30(5)：62-65.
25.胡厚堂,王海宁.生物吸附法处理水体中的重金属的现状与展望[J].新疆环境保护2003,25(4)：22-25.
26.Mohd Ikram Ansari,and Abdul Malik. Biosorption of nickel and cadmium by metal resistant bacterial isolates from agricultural soil irrigated with industrial wastewater[J].Bioresource Technology,2007,98(16):3149-3153.
27.邱廷省,成先雄.啤酒酵母吸附镉离子的试验研究[J].环境污染与防治,2004,26(2)：95-97.
28.Lodeiro P,Barriada J L,Herrero R,et al. The marine macroalga Cys-toseira baccata as biosorbent for cadmium(Ⅱ)and lead(Ⅱ)removal:Kinetic and equilibrium studies[J].Environmental Pollution,2005,15:110.
29.Hashim M A,Chu K H. Biosorption of cadmium by brown,green,and red seaweeds[J].Chemical Engineering Journal,2004,97:249-255.
30.叶锦韶,尹华,彭辉,等.重金属的生物吸附研究进展[J].城市环境与城市生态,2001,14(3)：30-32.
31.Chen J Z,Tao X C,Xu J,et al. Biosorption oflead, cadmium and mercury by immobilized Microcystis aeruginosa in acolumn[J].Process Biochemistry,2005,40(12):3675-3679.
32.Takata M,Matsuoka H,Ban H, et al. Biosynthesis of PHBV by mutant of Sphaerotilus natans [J],Appl. Microbiol. Biotech.,1995,44(2):37-42.
33.Saadet S, Osman G,Erman S,et al.Adsorption of Cd(Ⅱ),Cu(Ⅱ) and Ni(Ⅱ)ions by lemna minor L:Effect of physicochemical environment[J].Journal of Hazardous Materials,2005,126(1-2):96-104.
34.Christian V,Taty-costodes,Henri F,Catherine P,et al.Remaval of Cd(Ⅱ)and Pb(Ⅱ) ions fuo aqueous solution by adsorption onto sawdust of pinus sylvestris[J].Journal of Hazardous Materials,2003,105:121-142.
35.周东琴,魏德洲.草分枝杆菌及其吸附Pb2+后的可浮性研究[J].环境科学,2006,27(2)：338-342.
36.朱一民,苏秀娟,魏德洲,等.沉淀酵母菌对Pb(Ⅱ)的吸附机理研究[J].安全与环境学报,2006,6(6)：63-66.
37.苏秀娟,朱一民,沈岩柏,等.悬浮酵母菌对重金属Hg(Ⅱ)的吸附机理[J].安全与环境学报,2006,6(6)：63-66.
38.关晓辉,秦玉春,赵洁,等.浮游球衣菌去除废水中Pb2+的研究。环境科学,2005,26(6)：1107-1110.
39.P.Ligy, I.Leela, C.Venkobachar. Site of interaction of copper on Bacillus polymyxa[J].Water,Air and Soil Pollution,2000,119:11-21.
40.L.Asuncion, L.Naria, M. Susana et al. Nickel biosorption by free and immobilized cells of Pseudomonas fluorescens 4F39:A comparative study[J].Water, Air and Soil Pollution,2002,135:157-172.
41.N. Akira, Y. Masahide, Y.Hidekatsu et al. Copper biosorption by chemically treated Micrococcus Luteus cells[J].World Journal of Microbiology & Biotechnology,2001,7:343-347.
42.刘月英,傅锦坤,李仁忠,等.细菌吸附Pd2+的研究[J].微生物学报,2000,20(5)：535-539.
43.T. Suzuki, T. Hatsushika, A.Y. Hayakaw. Synathetic hydroxgapatite employed as inorganic cation exchangers[J].Journal of Chemical Society Faradary Transaction,1981,77:1059-1062.
44.E.R.El-Helos, S.Sabry,R.M. Amer. Cadmium biosorption by a cadium resistant strain of Bacillus thuringiensisi regulation and optimization of cell surface affinity for metaleation[J].Bio, Metals,2000,13:273-280.
45.陈明,赵永红.微生物吸附重金属离子的试验研究[J].南方冶金学院学报,2001,22(3)：168-173.
46.李明春,姜恒,候文强,等.酵母菌对重金属离子的吸附的研究.[J].菌物系统,1998,17(4).
47.Holan Z R, Volesky B. Biosorption of cadmidum by biomass of marine algae[J].Biotechnol. Bioeng, 1993,41:819-825.
48.Volesky B,Ciminelli VST.Assessment of interference in biosorption of heavy metals[J].Biotech.and Bioeng,1997,54(2):344-349.
49.牛慧,许学书,王建华.非生长产黄青霉菌吸附铅的研究[J].微生物学报,1993,33(6)：459-463.
50.李清彪,吴涓,杨宏泉,等.白腐真菌菌丝球形成的物化条件及其对铅的吸附[J].环境科学,1999,20(1)：33-38.
51.刘瑞霞,汤鸿霄,劳伟雄.重金属的生物吸附机理及吸附平衡模式研究[J].化学进展,2002,14(2)：87-92.
52.Nurgul B,Erdem K,Beyhan K.Biosorption of copper(Ⅱ) from aqueous solutions by wheat shell[J].Desalination,2004,164:135-140.
53.孟琴.生物吸附剂BAP对Cu2+的吸附研究[J].水处理技术,1998,24(3)：175-178.
54.吴乾青,李昕,李福德,等.微生物治理电镀废水的研究[J].环境科学,1997,9：47-50.
55.秦玉春,关晓辉,王立文,等.浮游球衣菌对Pb2+、Cu2+、Zn2+、d2+的吸附性能研究[J].环境污染与防治,2005,27(9)：648-650.
56.Tobin J M,Cooper D G.,Neufeld R J. Uptake of metal ions by Rhizopus arrhizus biomass[J],Appl. and Environ. Microbio.,1984,47(4):821-829.
57.Kuyucak N,Volesky B.Biosorbents for recovery of metals from industrial soluti ons[J],Biotechnol. Lett., 1988,10(2):137-142.
58.汤岳琴,林军,王建华.生物吸附研究进展[J],四川环境,2001,20(2)：12-16.
59.Muzzarelli R A A, Tubertini O. Chintin and chitosan as chromatographic supports and adsor-bents for collection of metal ions from organic and aqueous solutions and seawater[J].Talanta,1969,16:1571-1577.
60.Shumate ⅡS E,Strandberg G W.Accumulation of metals by microbial cells[J].New York:Pergamon Press Comprehensive Biotechnology,1985,4:235-239.
61.Ewa K, Francis A J. Immobilization of cadnium by microbial extracelluar products[J].Arch. Environ. Contam. Toxicol,1991,20:106-111.
62J.Yang, B. Volesky. Biosorption of uranium on sargassum biomass[J].Water Research,1999,33(15):3357-3363.
63.J.Wang. Biosorption of copper(Ⅱ)by chemically modified biomass of saccharomyces cerevisiae[J]. Process Biochemistry,2002,37(8):847-850.
64. Fourest, B. Volesky. Contribution of sulphonate guoups and alginate to heavymetal biosorption by the dry biomass of Sargassum fluitans[J].Environ Sci Technol,1996,30(1):277-282.
65.Y. Liu, J.K.Fu, R.Z. Li et al. Studies on biosorption of Pd2+ by bacteria[J].ActaMicrobio Sinn,2000,40(5):536-539.
66.lesky B.,May H.,Holan Z.R.,Biotech.and Bioeng.,1993,41,826.
67.ackwell K.J.,Singleton I.,Tobin J.M.,Appl.Microbiol.Biotechnol,1995,43,579.
68.黄民生,施华丽,郑乐平.曲霉对水中重金属的吸附去除[J].上海环境科学,2002,21(2)：89-92.
69.屠娟,张利,赵力,等.非活性根霉对废水中重金属离子的吸附[J].环境科学,1994,16(1)：12-15.
70.吴涓,李清彪,邓旭,等.白腐真菌吸附铅的研究[J].微生物学报,1999,39(1)：87-90.
71.周定.固定化细胞在废水处理中的应用及前景[J]环境科学,1998,14(5)：51-54.
72.A.M. Gaudin. Forth flotation-50th anniv[M].New York,Fuerstenau, D.W,1962,191.
73.A.I. Zouboulis, K.A. Matis, N.K. Lazaridis. Removal of metal ions from simulated wastewater by Saccharomyces yeast biomass:combining biosorption and flotation processes[J].Sep Sci Technol,2001,36:349-365.
74.K.A. Matis, G.P. Gallios, K.A. Kydros. Separation of fines by flotation techniques[J].Sep Technol,1993,3:76-90.
75.C.Alodrich, D.Feng. Removal of heavy metals from wastewater effluents by biosorptive flotation[J]. Minerals Engineering,13:1129-1138.
76.周东琴,魏德州.沟戈登氏菌对重金属的生物吸附-浮选和解吸性能[J].环境科学,2006,27(5)：961-964.
77.刘振扬,刘超.啤酒酵母在处理含镉工业废水中的应用研究[J].酿酒科技,2006(7)：85-88.
78.朱雪强,韩宝平.重金属生物吸附研究进展[J].中国环保产业,2004(5)：19-21.
79.Lodeiro P, Cordero B, Barriada J L, et al. Biosorption of cadmium bybiomass of brown marine macroalgae[J]. Journal of Hazardous Materials,2005,126(13):96-104.
80.陈国华.环境污染治理方法原理与工艺[M].北京：化学工业出版社,2003
81.Alena Luptakova, Maria Kusnierova. Bioremediation of acid minedrainage contaminated by SRB[J]. Hydrometallurgy,2005,77(1 2):97-102.
82.G Cabrera, RPerez, JMGomez, et al. Toxic effects of dissolved heavy metals on Desulfovibrio vulgaris and Desulfovibrio sp. strains[J].Journal of HazardousMaterials,2006,18:1-7.
83.D Fenga, CAldrich. Adsorption of heavy metals by biomaterials derived from the marine alga Ecklonia maxima[J].Hydrometallurgy,2004,73(12):1-10.
84.Pavel Kortrb,Lucie Doleckova,Vi ctor DeLorenzo,et al. Appl[J].Environ. Microbiol, 1999,65(3):1092-1098.
85.N Rangsayatorn, P Pokethitiyooka, E S Upatham, et al. Cadmiumbiosorption by cellsofSpirulina platensis TISTR8217 immobilized in alginate and silica gel[J].Environment International,2004,30(1):57-63.
86.MYakup Arlca, Gulay Bayramoglu, Meltem Yllmaz, et al. Biosorp-tion of Hg2+, Cd2+, and Zn2+ by ca alginate and immobilizedwoodrotting fungus Funalia trogii[J].Journal of HazardousMaterials,2004,109(1 3):191-199.
87.Kacar Y, Cigdem A, Sema T, et al. Biosorption of Hg(Ⅱ) and Cd(Ⅱ)from aqueous solutions: comparison of biosorptive capacity of alginate and immobilized live and heat inactivated Phanerochaete chrysosporium[J].Process Biochemistry,2002,37(6):601-610.
88.钱存柔,黄仪秀.微生物学试验教程[M].北京：北京大学出版社,2001.
89.辛宝平,陈桂淋,郑文钗.黑曲霉对弱酸性艳兰RAWL的吸附动力学和热力学研究[J].安全与环境学报,2006,6(5)：1-3.
90.马放,任南琪,杨基先.污染控制微生物学实验[M].哈尔滨：哈尔滨工业大学出版社,2002,6.
91.王金发,何炎明.细胞生物学实验教程[M].北京：科学出版社,2004,9.
92.Leo M/L.Nollet主编.袁洪福,褚小立,王艳斌,等译.水分析手册[M].北京：中国石化出版社,2005,6.
93.黄突.离子色谱仪的原理及应用[J].华中电力,2004,17(1)：69-70.
94.张建丽,刘志恒.诺卡氏型放线菌的分类[J].微生物学报,2001,4：513-517.
95.马邦亚,杨俊秀,曹支敏.放线菌分类研究的历史与现状[J].西北林学院学报,1999,14(3)：76-80.
96.徐成勇,袁野,黎丹辉,等.选择性分离放线菌[J].无锡轻工大学学报,1999,18(2)：45-49.
97.胥秀英,郑一敏,温寿祯,等.土壤放线菌分离方法的初步研究[J].生物学杂志,2000,17(2)：16.17.
98.杨宇容,徐丽华,李启任,等.放线菌分离方法的研究[J].微生物学通报,1995,22(2)：85-91.
99.吴襟,何秉旺.诺卡氏菌形放线菌β-甘露聚糖酶的纯化和性质[J].微生物学报,2000,40(1)：69-74.
100.H. Aimin, D.R. Simpson,L. Daniels et al. Cloning, expression, purification, and characterization of Nocardia sp. GTP cyclohydrolase I[J].Protein Expression & Purification,2004,35:171-180.
101.W. D. Kim, K. C. Daniel, J. Wang, et al. Heavy metal removal by activated sludge:influence of Nocardia amarae[J].Chemosphere,2002,46:137-142.
102.刘五星,徐旭士,杨启银,等.胶质芽孢杆菌发酵条件研究[J].南昌大学学报,2002,26(3)：299-302.
103.廖延雄,傅筱冲,蔡汝林,等.一株硅酸盐细菌的表型特征[J].江西科学,2000,18(3)：149-153.
104.王平宇,张树华.硅酸盐细菌的分离及生理生化特征的鉴定[J].南昌航空工业学院学报,2001,15(2)：78-82.
105.S.P. Vijayalakshmi, A.M. Raichur. Bioflocculation of high-ash India coals using Paenibacillus polymyxa[J].Mineral Processing,2002,67:199-210.
106. P.K. Sharma, K. Hanumantha Rao,K.S.E. Forssberg, K.A. Natarajan. Surface chemical characterisation of Paenibacillus polymyxa before and after adaptation to sulfide minerals[J].Mineral Processing,2001,62:3-25.
107.D. Santhiya, S. Subramanian, K.A. Natarajan. Surface chemical studies on sphalerite and galena using extracellular polysaccharides isolated from Bacillus polymyxa[J].Journal of Colloid and Interface Science,2002,256:237-248.
108.Namita Deo, K.A. Natarajan. Studies on interaction of Paenibacillus polymyxa with iron ore minerals in relation to beneficiation[J].Mineral Processing,1998,55:41-60.
109.王希成.生物化学[M].北京：清华大学出版社,2001.
110沈同.生物化学[M].北京：高等教育出版社,1999.
111.李建武.生物化学试验原理与方法[M].北京：北京大学出版社,1994.
112.B.R.格利克,J.J.帕斯捷尔纳克.分子生物技术[M].北京：化学工业出版社,2005.3.
113.D.W琼斯主编,江丕栋,等译.生物聚合物波谱学导论[M].北京：科学出版社,1983.
114.D.Lin-Vien.,N.B. Colthup.,W.G. Fateley et al. The handbook of infrared and raman characteristic frequencies of organie molecules[M].Academic Press,INC. San Diego. Canada,1991.
115.Van Loodrecht,M. C. M et al.The role of bacterial cell wall hydrophobicity in adhesion[J].Appl. Env. Microbiol,1987,53:1893-1897.
116.T.A. Stenstrom. Bacterial hydrophobicity, an overall parameter for the measurement of adhesion potential to soil particles[J].Appl. Env. Microbiol,1989,55:14-42.
117.G. P. Reid et al. Comparison of contact angles and adhesion to hexadecane of urogenital, dairy, and poultry lactobacillus:effect of serial culture passages[J].Appl. Env. Microbiol,1992,58:1549-1553.
118.N. Mozes,P.G. Rouxhet. Methods for measuring hydrophobicity of microorganism[J].Microbial. Methods,1987,6:99-112.
119.R.T. Neufeld et al. Cell surface measurements in hydrocarbon and carbonhydrate fermentations[J]. Appl. Env. Microbiol,1980,39:511-517.
120.方景礼.多元络合物电镀[M].国防工业出版社,1983,6.
121.汤岳琴,牛慧,林军,等.产黄青霉废菌体对铅的吸附机理研究[J],四川大学学报(工程科学版),2001,33(3)：50-54.
122.李志东,宋旭鹭,梁伟,等.啤酒酵母吸附重金属离子镉的研究[J].酿酒科技,2006,6：85-88.
123.刘刚,李清彪.重金属生物吸附的基础和过程研究[J].水处理技术,2002,28(1)：17-21.
124.马金鑫,朱国凯.扫描电子显微镜入门[M].北京：科学出版社,1985.
125.David J M,Alan J M,et al.The structure of a β-1,3-glucan from yeast cell walls[J].Biochem.J,1973B,135:11-18
126.朱淮武.有机分子结构波谱解析[M].北京：化工工业出版社,2005,9.
127.Bohn J A,Bemiller J N.(1→3)-β-glucans as biological response modifiers:a review of structure-funtional activity relationships[J].Carbohydrate Polymers,1995,28:13-14.
128.刘世宏,王当憨,潘成璜.X射线光电子能谱分析[M].北京：科学出版社,1988.
129.Zhong Y L,Jian M W,Ru X,et al.Spectroscopic characterization of Au3+ biosorption by waste biomass of saccharomyces cerevisiae[J].Spectrochimica Acta Part A,2005,61(6):761-765.
130.Pethkar A V,Kulkarni S K,Paknikar K M.Comparative studies on metal biosorption by two strains of cladosporium cladosporioides[J].Bioresource Technology,2001,80(3):211-215.
131.Dong H P,Yeoung S Y,Ji H J,et al.Mechanism of hexavalent chromium removal by dead fungal biomass of aspergillus niger[J].Water Research,2005,39(14):533-540.
132.Dao Z,Lina Z,Sheng L G.Mechanisms of lead biosorption on cellulose/chitin beads[J].Water Research,2005,39(16):3755-3762.
133.Subhashree P,Sarita S,Lal C R.Characterization of various functional groups present in the capsule of microcystis and study of their role in biosorption of Fe,Ni and Cr[J].Bioresource Technology,2007,98(3):595-601.
134.Shubo D,Yen-peng T. Characterization of PEI-modified biomass and biosorption of Cu(Ⅱ),Pb(Ⅱ) and Ni(Ⅱ)[J].Water Research,2005,39(10):2167-2177.
135.王恩德.环境资源中的微生物技术[M].北京：冶金工业出版社,1997.
136.有色金属冶金编辑委员会.中国冶金百科全书[M].北京：冶金工业出版社,1992.
137.汪贻水,王志雄,沈建忠.六十四种有色金属[M].长沙：中南工业大学出版社,1998.
138.四川大学化学信息中心.化学数据速查手册[M].化学工业出版社,华功电力出版中心出品,电子出版物数据中心.
139.Dudley H.Williams Ian Fleming著.王剑波,施卫峰译.有机化学中的光谱方法[M].北京：北京大学出版社2001,6.
140.岑沛霖,蔡谨.工业微生物学[M].北京：化学工业出版社,2000,6.
141.朱建光.浮选药剂[M].北京：冶金工业出版社,1993.