水稻无侧根突变体RM109的蛋白质组学研究
|
摘要
根系是植物体的一个重要组成部分,植物体正常生命活动所需要的水分和矿物质都是由根系从土壤中吸收而来,同时根系对植物体具有机械支撑作用。此外,根系还可以合成和分泌植物激素,对植物的生命活动具有重要的调节作用。发达的根系对植物体正常的生命活动及抵抗不良环境具有决定性的作用。侧根和根毛能够大幅度增加根系的吸收面积。其数量、分布及发育状况的好坏直接影响水肥的吸收,从而影响到作物在多种条件下的生长状况,最终影响作物的产量,因此研究侧根的发生机理对于农作物的高产具有重要的意义。
蛋白质是生物体结构和功能的单位,是基因功能的直接执行者。从遗传学的中心法则来看,基因和蛋白质之间应该存在着一一对应的线形关系。然而,事实并不象人们想象的那样,最近的研究表明,细胞中的实际蛋白质水平与转录水平之间缺乏相关性,DNA和RNA并不能准确反应蛋白质的表达、修饰及相互作用等生命科学的重要问题,因此要真正阐释生命的奥秘,需要对蛋白质进行大规模的全面研究,这就是蛋白质组学的目标和任务。
本实验采用双向凝胶电泳(2-DE)、2-DE凝胶分析软件、肽质量指纹等技术分析了突变体及其野生型,探讨了其过程中的一些影响因素,建立了比较完善的蛋白质组学研究方法。并重点研究了水稻无侧根突变体RM109及其野生型大力。分析其不同部位的双向电泳图谱的蛋白质表达差异,应用胰蛋白酶胶内酶解及基质辅助激光解吸/电离飞行时间质谱(MALDI-TOFMS)的肽质指纹技术对40个差异表达蛋白质进行鉴定,最终鉴定出20个蛋白,从而为侧根发生机理的研究提供科学依据。
Plant roots serve important functions.The root of terrestrial plant are involved in the acquisition of water and nutrient in the soil, anchange of plant, synthesis and secretory of plant hormones and regulation of life activity .The development of a root system involves strategies that are common to the development of all plant organ.Lateral roots and root hair can increas root absorbing area directly. The amount and development of lateral roots also can affect the absorbing of water and nutrients, whatsmore, affect the product of food. So it is very important to study the mechanism of lateral root.
Protein is the unit of biology structure and function. According to the central rules, it seems that there has a linear relation between gene and protein. However, it is not as much as people think. Recently, the level of protein and transfer in cell is lack of ralitivity. DNA and RNA are unable to reflect the expression, modification and reciprocity of protein correctly. Thus, to reveal the mystery of life, the aim of the proteomics is to study widly and cosmically.
We analyse groups of mutants and their wild type, use a series of methods, including immobilized pH gradient two dimensional polyacrylamide gel electrophoresis, commassio staining, PDQuest 2-DE software, peptide mass fingerprinting based on MALDI TOF MS and swiss-PROT database searching and set up a proteomic approach. We emphasized to analyse proteomic of lack-lateral-root mutant and its wild type. Comparision of their maps, 40 different spots were digested in gel with TPCK-trypsin and measured with MALDI TOF MS, and 20 protein were identified, which may help to search mechanism of the development of lateral root.
蛋白质是生物体结构和功能的单位,是基因功能的直接执行者。从遗传学的中心法则来看,基因和蛋白质之间应该存在着一一对应的线形关系。然而,事实并不象人们想象的那样,最近的研究表明,细胞中的实际蛋白质水平与转录水平之间缺乏相关性,DNA和RNA并不能准确反应蛋白质的表达、修饰及相互作用等生命科学的重要问题,因此要真正阐释生命的奥秘,需要对蛋白质进行大规模的全面研究,这就是蛋白质组学的目标和任务。
本实验采用双向凝胶电泳(2-DE)、2-DE凝胶分析软件、肽质量指纹等技术分析了突变体及其野生型,探讨了其过程中的一些影响因素,建立了比较完善的蛋白质组学研究方法。并重点研究了水稻无侧根突变体RM109及其野生型大力。分析其不同部位的双向电泳图谱的蛋白质表达差异,应用胰蛋白酶胶内酶解及基质辅助激光解吸/电离飞行时间质谱(MALDI-TOFMS)的肽质指纹技术对40个差异表达蛋白质进行鉴定,最终鉴定出20个蛋白,从而为侧根发生机理的研究提供科学依据。
Plant roots serve important functions.The root of terrestrial plant are involved in the acquisition of water and nutrient in the soil, anchange of plant, synthesis and secretory of plant hormones and regulation of life activity .The development of a root system involves strategies that are common to the development of all plant organ.Lateral roots and root hair can increas root absorbing area directly. The amount and development of lateral roots also can affect the absorbing of water and nutrients, whatsmore, affect the product of food. So it is very important to study the mechanism of lateral root.
Protein is the unit of biology structure and function. According to the central rules, it seems that there has a linear relation between gene and protein. However, it is not as much as people think. Recently, the level of protein and transfer in cell is lack of ralitivity. DNA and RNA are unable to reflect the expression, modification and reciprocity of protein correctly. Thus, to reveal the mystery of life, the aim of the proteomics is to study widly and cosmically.
We analyse groups of mutants and their wild type, use a series of methods, including immobilized pH gradient two dimensional polyacrylamide gel electrophoresis, commassio staining, PDQuest 2-DE software, peptide mass fingerprinting based on MALDI TOF MS and swiss-PROT database searching and set up a proteomic approach. We emphasized to analyse proteomic of lack-lateral-root mutant and its wild type. Comparision of their maps, 40 different spots were digested in gel with TPCK-trypsin and measured with MALDI TOF MS, and 20 protein were identified, which may help to search mechanism of the development of lateral root.
引文
1 A.B.Krech D, Wulff K D, Grasser and G. Feix,Plant chromosomal HMGI/Y proteins and histone H1exhibit a protein domain of common origin,April Gene,1999,230:1～5
2 Anders M Lindroth,Peter Saarikoski, Gunnar Flygh,etal. Two S-adenosylethionine synthetseencoding genes differentially expressed duringadventitious root development in Pinus contor- ta. Plant Molecular Biology ,2001,46:335～346
3 Anderson NG,Anderson NL,Twenty years of two-dimensional electrophoresis :Past, present and future[J],Electrophoresis,1996,17(3):443～453
4 Anderson NL,Anderson NG,Proteome and prteomics:new technologies,new concepts, and new words,Electrophoresis,1998,19:1853～1861
5 Bcerjan W, Cervera M T ,Delarue M, Beeck man T,Superroot,a recessive mution in Arabid opsis,confers ausin overproduction ,Plant cell,1979,7:1405～1419
6 Beeck man T, Burssens S, Inze D. The peri-cell-cycle in Arabidopsis. Journal of Experim- ental Botany, 2001,52:403～411
7 Bell J K,McCully M G. Ahistological study of lateral root initiation,development in Zea mays.Protoplasma,1970,70:179～205
8 Beyer K, Bardina L, Grishina G, Sampson HA.,Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: seed storage proteins as common food allergens,J Allergy Clin Immunol, 2002, 110(1):154～159
9 Biermann B, Feldman LJ.,Physiological, biochemical and molecular processes associated with gravitropism in roots of maize, Adv Space Res,1994:14(8):331～340
10 Bjellqvist B, Sanchez JC, Hochstrasser DF, etal. Micropreparative two dimensional electro- phoresis allowing the separation of samples containing milligramamounts of proteins, Elec- trophoresis,1993,14:1375～1378
11 Blakely L M,Durham m,e vans T A,Blakely R M.Experimental studies on lateral root form- ation in radish seeding roots.General metheds,developmental stages, spontaneous formation of laterals.Bot Gaz, ,1982,143(3):341～352
12 Blakely L M,Evans T A, Cell dynamics studies on the pericycle of radish seeds roots,Plant Sci Lett,1982,14:79～83
13 Carlos M. Vicient, Natacha Bies-Etheve and Michel Delseny ,Changes in gene expression in the leafy cotyledon1 (lec1) and fusca3 (fus3) mutants of Arabidopsis thaliana L, Journal of Experimental Botany, 2000,347:995～1003
14 Casero P J,Casimiro T, Rodnguez-Callardo L,Martin-Partido G,Lateral root initation by means of a transversal divisions of the pericycle cells in adventitioous roots of Alliumcepa , Protoplasma,1993,176:138～144
15 Casero P J,Casimiro T,Loret P G,Later root initiation by means of asymmetrical transversal divisions of the percycle cells in four different species of plants:Raphanus sativus, Helianth- us annuns, Zea mays,Daucus carota, Protoplasma,1995,188:49～58
16 Casimiro T, Marchant A, Bhalerao R P, Beeck man T,Dhooge S, Auxin transport promotes Arabidopsis lateral root initiation,Plant Cell,2001,13:843～852
17 Castellanos Serra LR, Fernandez Patron C, Huerta V,etal.A procedure for protein elution from reverse stained polyacrylamide gels applicable at the low picolmole level:analternative route to the preparation of low abundance proteins formicroanalysis. Electrophoresis, 1996, 17:1564～1572
18 Celenza J,Grisafi P L,Fink G R, A pathway for lateral root formation in Arabidopsis thaliana,Gene Dev,1995,9:2131～2142
19 Celis J E,Gromov P.2-D protein electrophoresis :Canitbe perfected [J] Curr Opin Biotechnol ,1999,10(1):16～21
20 Chait BT, Wang R, KentS BH,etal. Protein ladder sequencing.Science,1993,62:89～92
21 Chambers G,Lawrie L,Cash P,Murray G I.Proteomics:A new approach to the study of disease[J].J Pathol,2000,192(3):280～288
22 Cheng J C ,Seeley K A, Sung Z A , Arabidopsis genes required for cell proliferation at the root tip ,Plant Physiol,107 :365～376
23 Chevallet M, Santoni V, Rabilloud T, etal. Newzwitter ionic detergents improve the analysis of membrane proteins by two dimensional electrophoresis. Electrophoresis, 1998,19:1901～1909
24 Christine Finnie,Sabrina Melchior,Peter Roepstorff, Birte Svensson,Proteome Analysis of Grain Filling and Seed Maturation in Barley,Plant Physiol, 2002, 129 : 1308～1319
25 Constance N, White,Carol J, Rivinn,Gibberellins and Seed Development in Maize, Gibb- erellin Synthesis Inhibition Enhances Abscisic Acid Signaling in Cultured Embryos. Plant Physiol, 2000, 122:1089～1098
26 Constance N. White,William M. Proebsting, Peter Hedden, Gibberellins and Seed Development in Maize. I.?Evidence That Gibberellin/Abscisic Acid Balance Governs Germination versus Maturation Pathways,April Plant Physiol,2000, (122):1081～1088
27 Cremer F,Van de Wallec. Methods for Extraction of Proteins from Green Plant Tissues for Two-Dimensional, Polyacrylamide Gel Electrophoresis.Anal Biochem,1985,147: 22～26
28 Dcerner P, Cell cycle regulation in plants ,Plant Physiol,1994,106:823～827
29 Dcerner P, J rgensen J E,You R,Steppuhn J,Control of root growth,development by cyclin expression ,Nature ,1996,380:520～523
30 Di Layrenzio L,Wysocka-Diller J,Malamy J E,Pysh L,Helariutta Y,Freshour G,Hahn M G,The SCARECROW gene regulates an asymmetric cell division that is essential for generating the radial organization of the Arabidopsis root,Cell,1996,423～433
31 Dittmer H J,A quantitative study of the roots, root hairs of a winter rye plant ,Amer J Bot, 1987,24:417～420
32 Dolan L,Janmaat K,Willemsen V,Linstead P, Pcethig S,Roberts K,ScheresB,Cellular organ- isation of the thaliana root ,Development,1993,119:71～84
33 Dominique Van Der Straeten,Renato A Rodrigues-Pousada,Howard M.Goodman, etal Plant Enclose:Gene structure,Expression,and Evolution,Plant Cell, 1991, 3 : 719～735
34 Dubrovsky J G,Dcerner P W,Colon-Carmona A, Rost T L,Pericycle cell proliferation , lateral root initation in Arabidopsis Plant Physiol ,2000,124:1648～1657
35 Ferreira P C G, Hemerly A S, de Almeida, Van Montagu M, Engler G, Developmental expression of the Arabidopsis cyclin gene cyc1At,Plant Cell,1996,6:1763～1774
36 Figeys D, Aebersold R. High sensitivity identification of proteins by electrospray ionization tandem mass spectrometry: initial comparison between an ion trap mass spectrometer and a triple quadrupole mass spectrometer. Electrophoresis, 1997,18: 300～368
37 Figeys D,McBroom L D,Moran M F.Mass spectrometry for the study of protein interactions [J],Methods,2001,24(3)230～239
38 Frank Hochholdinger, Woong June Park, Günter H. Feix. Cooperative Action of SLR1 and SLR2 Is Required for Lateral Root-Specific Cell Elongation in Maize. Plant Physiol, 2001, 125:1529～1539
39 Frank Hochholdinger,GunteFeix.Early post-embryonic root formation is specifically affected in the maize mutant lrt1.1998,Plant Journal,16(2):247～255.
40 Fukaki H,Fujisawa H,Tasaka M,SGR1.SGR2,SGR3:novel genetic loci involved in shoot gravitropism in Arabidopsis ,Plant Physiol,1996,100:945～955
41 Fukali H,Wysocka-Diller J W,Kato T,Fujisawa H,Benfey P N,Tasaka M,Genetic evidence that endodermis is essential for shoot gravitropism in Arabidopsis thaliana, Plant J,1998, 14:425～430
42 Gaskell SJ. Electrospray: principles and practice. Journal of Mass Spectrometry, 1997,32: 677～688
43 Gorg A, Obermaier C, Madjar JJ. Very alkaline immobilized pH gradients for two dimensional electrophoresis of ribosomal and nuclear proteins, Electrophoresis, 1997,18:328～337
44 Gorg A, Postel W,Gunther S.The current state of two dimensional electophoresis with inmobilized pH gradient.Electrophoresis,1988,9:531～546
45 Gorg A,Obermaier C,Boguth G,Harder A,Scheibe B,Wild-gruber R,Weiss W. The current state of two-dimensional electrophoresis with immobilized pH gradients [J]. Electrophore- sis,200 ,21(6):1037～1053
46 Griffin T J, Goodlett D R, Aebersold R, Advances in proteome analysis by mass spectrometry [J].Curr Opin Biotechnol,2001,12(6):607～612
47 Gygi SP,Corthals GL,Zhang Y,Rochon Y,Aebersold R.E-valuation of two- dimensional gel electrophoresis-based proteome analysis technology[J].Proc Natl Acad Sci USA, 2000, 97 (17) :9390～9395.
48 HaoZai-bin, LanXiao-xia, YangLiang-qun. Plant Hormone Resistance and Agronomic Characteristics of the MT10 Mutantin Rice, Journal of Northeast Agricultural University 2000,7 (2):130～135
49 HaoZB,XuZH,CangJ,IchiiM.MT10 Mutant of Rice with Altered Lateral Root Initiation. Journal of Northeast Agricultural University,2000,7(7):38～42
50 Haynes PA, Gygi SP, Aebersold R, etal. Proteome analysis:biological assay or data archive Electrophoresis,1998,19:1862～1871
51 Heck GR, Chamberlain AK, Ho TH. Barley embryo globulin 1 gene, Beg1: character- ization of cDNA, chromosome mapping and regulation of expression. May; Mol Gen Genet, 1993, 239(1-2):209～218
52 Hemerly A S,Ferveira P,de Almeida,Engler G,lnze D,Cdc2a experession in Arabidopsis is linked with competence for cell dvision ,Plant Cell,1993,5:1711～1723
53 Henzel WJ, Billeci TM, Watanae C, etal. Identifying proteins from two dimensional gels
by molecular mass searching of peptide fragments in protein sequence database, Proc Natl Acad Sci USA,1993,90:5011～5015
54 Hidehiko Kikuchi,Sakiko Hirose, Seiichi Toki,etal. Molecular characterization of a gene for alanine aminotransferase from rice, Plant Molecular Biology,1999, 39: 149～159.
55 Hirano H.Microsequence analysis of winged bean seed proteins electroblotted from two-dimensional gel,J Protein Chem, 1989 ,8(1):115～130.
56 Hobbie L,Estelle M,The axr4 auxin-resistant mutants of Arabidopsis thaliana define a gene important for root gravitropism,lateral root initiation , Plant J,1995,7:211～220
57 Hochholdinger F,Park W J,Feix G H,Cooperative action of SLR1,SLR2 is required for lateral root–specific cell elongation in maize,Plant Physiol,2001,125:1529～1539
58 Holly Hilton,Brandon S,Gaut,Speciation and Domestication in Maize and Its Wild Relatives: Evidence From the Globulin-1 Gene. Genetics, 1998, 150:863～872
59 Humphery Smith I,Cordwell SJ,Proteom research:comple mentarity and limitations respect to the RNA and DNA worlds,Electrophoresis,1997,18:1217～1242
60 John W.Schiefelbein,Philip N.Benfey,The Development of Plant Roots:New Approa- ches to Undergroud Problems, Plant Cell, 1991, 3:1147～1154
61 Jungblut P,Thiede B,Protein identification from 2-DE gels by MALDI mass spectr- ometry. Mass Spectrometry reviews,1997,16:145～162
62 Keller B,Lamb CJ,Specific wxpression of a novel cell wall hydroxyproline–rich glycoprotein in lateral root initiation, Genes Dev, 1989,3:1639～1649
63 Karine Gallardo,Claudette Job,Steven P.C.Groot,etal.Proteomics of Arabidopsis Seed Germination.AComparative Study of Wild-Type and Gibberellin-Deficient Seeds, Plant Physiol 2002,129:823～837
64 Karlowski W M, Hirsch A M,The over-expression of an alfalfa RING-H2 gene induces pleiotropic effects on plant growth and development,Plant Mol Biol, 2003, 52(1): 121～133
65 Konishi M, Sugiyama M.,Genetic analysis of adventitious root formation with a novel series of temperature-sensitive mutants of Arabidopsis thaliana, Development, 2003, 130 (23):5637～5647
66 Jocelyn E. Malamy, Katherine S. Ryan ,Environmental Regulation of Lateral Root Initiation in Arabidopsis,Plant Physiol, 2001,127: 899～909
67 Kikuchi H, Hirose S, Toki S, Akama K, Takaiwa F,Molecular characterization of a gene for alanine aminotransferase from rice (Oryza sativa),Plant Mol Biol,1999, 39(1):149～159
68 Van der Straeten D, Rodrigues-Pousada RA, Goodman HM, Van Montagu M.,Plant enolase: gene structure, expression, and evolution.Plant Cell,1991,3(7):719～735
69 Kikuchi H., Hirose S., Toki S , Akama,K, Takaiwa F,Molecular characterization of a gene for alanine aminotransferase from rice (Oryza sativa), Plant Mol. Biol, 1999, 39 (1): 149～159
70 King J J,Stimart D P, Fisher R H,Bleecker A B,A mutation altering auxin homeostasis, Plant morpho;ogy in a Arabidopsis,Plant Cell,1995,7:2023～2037
71 Klee J H,The effects of overproduction of two Agrobacteriumtumefaciens T-DNA auxin
biosynthetic gene products in transgenic petunia plants ,Genes Dev ,1987,1:86～96
72 Klose J,Kobalz U,Two dimensional electrophoresis of proteins:an updated protocol and implications for a functional analysis of the genome, Electrophoresis,1995, 16: 1034～1059
73 Lal SK, Johnson S, Conway T, Kelley PM.Characterization of a maize cDNA that complements an enolase-deficient mutant of Escherichia coli. May, Plant Mol Biol, 1991, 16 (5) :787～795
74 Larkin P J, Gibson J M, Mathesius U, Weinman J J, etal.Transgenic white clover. Studies with the auxin-responsive promoter, GH3, in root gravitropism and lateral root development. Transgenic Res,1996,5(5):325～335
75 Tsutomu Suzuki, Maki Terasaki, Chie Takemoto-Hori,etal.Proteomic Analysis of the Mam- malian Mitochondrial Ribosome. J. Biol. Chem., 2001, 276,(35):33181～33195
76 Liu W, Xu ZH, Luo D, Xue HW.,Roles of OsCKI1, a rice casein kinase I, in root developm- ent and plant hormone sensitivity,Plant J,2003 ,36(2):189～202
77 Laskowski M J,Williams M E,Nusbaum H C,Sussex T M,Formation of laeral root meristems is a two-stage process ,Development ,1995,121:3303～3310
78 Latter GI, Burbeck S,Leavitt J,etal. Identification of polypep tides on two dimensional electrophoresis gels by aminoacid comparison.ClinChem,1984,30:1925～1932
79 Lien B. Lai, S. Lorraine Tausta, and Timothy M. Nelson .Differential Regulation of Transcripts Encoding Cytosolic NADP-Malic Enzyme in C3 and C4 Flaveria Species, Plant Physiol, 2002, 128:. 140～149
80 Liepman AH, Olsen LJ.,Alanine aminotransferase homologs catalyze the glutamate: Glyoxylate aminotransferase reaction in peroxisomes of Arabidopsis, Plant Physiol, 2003,131 (1) :215～227
81 Lincoln,C.,J.H.Britton,M.Estelle.Growth and development of the axr1 mutants of Arabidopsis.PlanntCell, 1990 (2):1071～1080
82 Link AJ.Auto radio graphy of 2-D gels[J],Methods Mol Biol,1999,112:285～290
83 Litts J C, Colwell G W, Chakerian R L, Quatrano R S,The nucleotide sequence of a cDNA clone encoding the wheat Em protein, Nucleic Acids Res, 1987,15(8):3607～3618
84 Nambara E, Hayama R, Tsuchiya Y, Nishimura M,etal.The role of ABI3 and FUS3 loci in Arabidopsis thaliana on phase transition from late embryo development to germination. Dev Biol. 2000, 220(2):412～423
85 Lloret P G,Casero P J,Puglann A,Navascues J,The behavious of two cell populations in the pericycle of Alliumcepa ,Pisum sativum,Daucus carota during early lateral root developme- nt,Ann Bot,63:465～475
86 LooJ A,DeJohn DE,Du P,Stevenson TI,OgorzalekLoo RR,Application of mass spectro- metry for target identification and characterization [J]. Med Res Rev,1999, 19 (4):307～319
87 Lourdes Gomez-Gomez, Pedro Carrasco. Differential Expression of the Saenosyi Lmethionine Synthase Genes during Pea Development, Plant Journal, 1998,117: 397～ 405
88 Lourdes Gómez-Gómez,Pedro Carrasco. Differential Expression of the S-Adenosyl-L- Methionine Synthase Genes during Pea Development,Plant Physiol, 1998, 117: 397～405
89 Luerssen H, Kirik V, Herrmann P, Misera S.FUSCA3 encodes a protein with a conserved VP1/AB13-like B3 domain which is of functional importance for the regulation of seed maturation in Arabidopsis thaliana, Plant J, 1998, 15(6):755～764
90 Lyford W H,Rhizography of non-woody roots of trees in the forest floor,In:Torrey J,The deve- lopment ,function of roots,New York:Academic Press:179～196
91 Malamy J E,Benfey P N,Organization,cell differentiation in lateral roots of Arabid- opsis thaliana,Development,1997,124:33～44
92 MalamyJ E, Benfey P N,Down,out in Arabidopsis:the formation of lateral roots,Trends Plant Sci,1997,2(10):390～396
93 Mann M,Hendrickson RC,Pandey A.Analysis of proteins and proteomes by mass spectrometry[J].Annu Rev Biochem,2001,70:437～473
95 Markus L, Goddemeier , Günter Feix,Genomic structure of the maize TATA-box binding protein 1 (TBP-1): conserved exon/intron structure in eukaryotic TBP genes, 1996 ,26: 111～114
96 Meyerowitz E M,Prehistory,history of Arabidopsis research ,Plant Physiol, 2001,125: 15～19
97 Muday G K,Haworth P,Tomato root growth,gravitropism,lateral development: coorela- tion with auxin transport,Plant Physiol Biochem,1994,32:193～203
98 Muench D G, Good A G.,Hypoxically inducible barley alanine aminotransferase: cDNA cloning and expression analysis,Plant Mol Biol,1994,24(3):417～427
99 Mullner S,Neumann T,Lottspeich F,Proteomics—a new way for drugtarget discovery, Arzneim Forsch/Drug Res,1998,48(1):93～95
100 O’ Farrel P H. High-resolution two–dmensional electrophoresis of proteins, J Biol Chem,1975, 250: 4007～4021
101 Ong SE,Pandey AB.An evaluation of the use of two-dimensional gel electrophor- esis in proteomics[J],Biomol Eng,2001,18(5):195～205
102 Pandey A,Mann M.Proteomics to study genes and genomes[J], Nature,2000, 405 (6788):837～846
103 Proteomic Analysis of the Mammalian Mitochondrial Ribosome, Biol. Chem. 2001,276(35) 33181～33195
104 Qiaoping Yuan, Feng Liang, Joseph Hsiao,etal. Anchoring of rice BAC clones to the rice genetic map in?silico, Nucleic Acids Research, 2000, 28(18): 3636～3641
105 Rabbilloud T,AdessiC,LunardiJ,etal.Improvement of the solubilization of proteins in two dimensional electrophoresis with immobilised pH gradients.Electrophoresis,1997, 18:307～316
106 Rabbilloud T. Use of thiourea to increase the solubility of membrane proteins in two dimensional electrophoresis. Electrophoresis,1998,19:758～760
107 Rabilloud T, Valette C, Lawrence JJ. Sample application by in gel rehydration improves the resolution of two dimensional electrophoresis with immobilized pH gradients in the
first dimension. Electrophoresis,1994,15:1552～1558
108 Rabilloud T.Silver staining of 2-D electrophoresis gels [J]. Methods Mol Biol, 1999,112:297～305
109 Rashotte A M,Brady S R,Reed R C,Ante S J,Muday G K,Basipetal auxin transport is required for gravitropism in roots of Arabidopsis ,Plant Physiol,2000,122:481～491
110 Reed R C,Brady S R,Muday G K,Inhibition of auxin movement from the shoot into inhibits lateral root development in Arabidopsis,Plant Physiol,1998,118:1369～1378
111 Rodrigues-Pousada R A, De Rycke R, Dedonder A, etal, The Arabidopsis 1-Amino cyclopro pane-1-Carboxylate Synthase Gene 1 Is Expressed during Early Development, Plant Cell. 1993,5(8):897～911
112 Rogg L E,Lasswell J,Bartel B,A gain-of-function mutation in IAA28 suppresses lateral root development ,Plant Cell,2001,13:465～480
113 Ruegger M,Dewey E,Hobbie L,Brown D,Bernasconi P,Turner J,Muday G,Estelle M,Reduced naphthylphthalamic acid binding in the tir3 mutant of Arabidopsis is associated with a reduction in polar auxin transport, diverse mor phological defects, Plant cell,1997,9:745～757
114 Sanchez JC, Appel RD, Hochstrasser DF. Inside SWISS 2-D PAGE database, Electrophoresis,1995,16:1131～1151
115 Scheres B, Mc Kahnn H I,Van den Berg C, Roots redefined: anatomical, genetic analysis of root development, Plant Physio,1999,111:959～964
116 Shailesh K. Lal, Chwenfang Lee, and Martin M. Sachs.Differential Regulation of Enolase during Anaerobiosis in Maize, Plant Physiol,1998,118: 1285-1293
117 Sharon Rozovsky, Gerwald Jogl, Liang Tong, Ann E. McDermott,Solution-state NMR investigations of triosephosphate isomerase active site loop motion: ligand release in relation to active site loop dynamics,Journal of Molecular Biology, 2001,310,(1) 271～280
118 Shevchenkenko A, Jensen ON,Mann M, etal. Linking genome and proteome by mass spectrometry large scale identification of yeast proteins form two dimensional gels. Proc Natl Acad Sci USA,1996,93:14440～14445
119 Smith D L,Fedoroff N V,LRPI,a gene expressed in lateral, adventitious primodia of Arabido psis,Plant Cell,1995,7:735～745
120 Takahiro Adachi,Hidehiko Izumi,Takehisa Yamada,etal.Gene structure and expression of rice seed allergenic proteins belonging to the α-amylase/trypsin inhibitor family, 1993, Plant Molecular Biology 21:239～248
121 Thomas C, Meyer D, Wolff M, Himber C, Alioua M, Steinmetz A.Molecular character- ization and spatial expression of the sunflower ABP1 gene,Plant Mol Biol,2003, 52 (5): 1025～1036
122 Timpte C,Lincoin C,Pickett F B,Turner J,Estelle M,The AXRI,AVXI genes of Arabid- opsis function inseparate auxin-response pathways,Plant J,1995,8:561～569
123 Timpte,C.,A.K.WilsonandM.Estelle.Theaxr2-1mutation of Arabidopsis thalianain a gain of function mutation that disruptsa nearly step inauxin response.Genetics, 1994, (138):1239～1249
124 Tonella L, Walsh BJ, Hochstrasser DF, etal.’98 Escherichia coli SWISS 2-D PAGE database update.Electrophoresis,1998,19:1960～1971
125 Torrey J G,The induction of lateral roots by indole acetic acid, root decaitation,Am J Bot, 1950,37:257～264
126 Traini M,Gooley AA, Williams LK, etal.Towards an automated approach for protein in identification proteome projects. Electrophoresis,1998,19:1941～1949
127 Tsuji H, Hiemori M, Kimoto M,etal. Cloning of cDNA encoding a soybean allergen, Gly m Bd 28K,Biochim Biophys Acta,2001,19;1518(1-2):178～182
128 Urquhart BL,Atsalos TE,Humphery Smith I,etal.Proteomic contigs of Mycobacterium tube rculosis and mycobacterium bovis(BCG) using novel immobilised pH gradients. Electrophoresis,1997,18:1384～1392
129 Van de Sande K,Modification of phytohormone response by a peptide encoded by ENOD40 of legumes,a nonlegume,Science,1996,273:370～373
130 Vernoux T,Wilson R C,Seeley K A,Reichheld J P,Muroy S,Brown S,Maughan S C, Cobbett C S,The ROOT MERISTEMLESS1/CADMIUM SENSITIVE 2GENE defines a glutathione dependent pathway involved in initiation,maintenance of cell division during postembryo- nic root development,Plant Cell,2000,12:97～109
131 Wang S C, Ichii M,Taketa S,Xu L L,Xia K,Zhou X,2002,Effect of jasmonic acid on lateral root formation in rice seedling. Acta Botanca Sinica,44(4):502～504
132 Wang S C,Ichii M,Taketa S,Xu L L,Xia K, Zhou X,2002b.Lateral root formation in rice SEEDLING:promotion effect of jasmonic acid.Journal of Plant Physionlogy, 159: 827～832
133 Wang Shu-Cai,Masshiko Ichii,Shin Taketa. Effect of Jasmonic Acid on Lateral Root Formation in Rice seedling,Acta Botanica Sinica, 2002,44(4):502～504
134 Waterborg JH,Matthews HR,Fluorography of polyacryl amide gels containing tritium[J],Methods Mol Biol,1994,32:163～167
135 Wightman F, Thimann K V,Hormonal factors controlling the initiation,development of lateral roots,Source of primodiuminducing substances in the primary root of pea seedings,Physiol Plant,49:13～20
136 Wilkins M R,Sanchez J C,Williama K L, Progress with proteome projects: Why allproteins expressed by agenome should bei dentified and how to do it ,Biotechnology and Genetic Engineering Reviews,1995,13:19～45
137 Willemsen V,Wolkenfelt H,de Vrieze G,Weisbeek P,Scheres B,The HOBBIT gene is required for formation of root meristem in the Arabidopsis embyryo, Development, 1998,125:521～531
138 Winfried Hetz,Frank Hochholdinger,Michael Schwall,Gunter Feix. Isolation and characterization of rtcs,a maize mutant deficient in the formation of nodal roots, Plant Journal, 1996,10(5):845～857
139 Wirth PJ,Hoang TN, Benjamin T. Micropreparative immobilezed pH gradient two dimensional electrophoresis incombination with protein microsequecing for the analysis of humanliver proteins. Electrophoresis,1995,16:1946～1960
140 Wu J., Cramer C.,Hatzios K K.,Isolation of a full-length cDNA encoding the second glutathione S-transferase from rice ,Plant Physiol,1998,118 (1), 329
141 Wysocka-Diller J W,Helariutta Y,Fukaki H,Malamy J E,Benfey P N,Molecular analysis of SCARECROW function reveals a radial patterning mechanism common to root, shoot. Development,2000,127:595～603
142 Xu Y, Hall,T C ,Cytosolic triosephosphate isomerase is a single gene in rice,Plant Physiol, 1993, 101 (20):683～687
143 Xu Y, Hall,T C, The induction of lateral roots by indole acetic acid, root decaitation,Am J Bot, 1950,37:257～264
144 Xu,Y,Harris-HallerL.W.,McCollum J.C.,etal.Nuclear gene encoding Cytosolic Trio- sephosphate isomerase from rice.1993,Plant Physiol. 102 (2): 697
145 Yates JR. Mass spectrometry and the age of the Proteome. Journal of mass spectro- metry, 1998,33:1921～1930
146 Yong Xu,Timothy C,Hall.Cytosolic Triosephosphate Isomerase Is a Single Gene in rice, Plant Physiol, 1993,101:683～687
147 成海平,钱小红,蛋白质组研究的技术体系及其进展,生物化学和生物物理进展, 2000, 27(6):584～588
148樊跃平,于健春,余跃,张琳,谷胱甘肽的生理意义及其各种测定方法比较、评价,中国临床营养杂志,2003,11(3):136～139
149 高原,王国秀,刘中来,一种经济、简便的双向电泳方法,生物化学与生物物理进展,2003,30(1):156～158
150 谷瑞升,刘群录,陈雪梅,蒋湘宁,一种省时高效的木本植物蛋白双向电泳分析方法,北京林业大学学报,1999,21(5):7～10
151 胡金勇,曾英,桑玉英,双向电泳分析鸢尾绿白嵌合叶片的蛋白质,云南植物研究。 2002,24 ( 3) : 387～391
152 黄英金,熊伟,漆映雪,刘宜柏,稻米蛋白质及其4种组分含量的遗传研究,江西农业大学学报,2000,22(4):473～478
153 贾宇峰,林秋霞,郭尧君,等.蛋白质双向电泳图像分析[J].生物化学与生物物理进展, 2001,28(2):246～250
154 李伯良,李林,吴家睿.功能蛋白质组学[J].生物工程进展,1999,19(4):15～16.
155 李林,吴家睿,李伯良.蛋白质组学的产生及其重要意义[J].生命科学,1999,11(2): 49～50
156 李林.蛋白质组学的进展[J].生物化学与生物物理学报,2000,27(3):227～231.
157梁五生,毛碧增,李帅,庄芳蕾,李德葆,水稻种子萌发过程中氰丙氨酸合酶的活性变化与分布,植物生理学报, 2001,27(3):243～247
158 廖祥儒,刘小丽,陈彤,袁京云,李业英,王俊丽,杜建芳,谷胱甘肽对小麦幼穗胚性愈伤组织形成以及几种相关酶活性的影响,植物生理学通讯,2002,38(4):327～329
159 逯斌,林兵.一种改良的植物蛋白质双向电泳方法.生物化学与生物物理进展,1989, 16(6):480～481
160 毛君铃,从双向电泳到生物质谱—蛋白质组分析技术的最新进展,现代科学仪器2001,2:37～38
161 戚元成,张慧,赵彦修,植物谷胱甘肽转移酶和盐胁迫,山东师范大学学报(自然科学版),2002,17(2):71～75
162 阮禹松,赵文明,种子盐溶球蛋白的结构特征 ,西北植物学报,2002,22(4):999～1003
163 万晶宏,贺福初.蛋白质组技术的研究进展,科学通报,1999,44(9):904～910
164 王台.一种适用于双向电泳凝胶的染色方法.植物学通报,1996,13(4):56～57
165 王新,王伯良,张宗友,等.蛋白质组分析中双向电泳技术的改良和应用,细胞与分子免疫学杂志,2001,17(2):197～199
166 吴庆如,黄开勋,徐辉碧,双向凝胶电泳技术进展[J],生物技术通讯,2002,13(2): 28～31
167 杨何义,应万涛,钱小红,蛋白质组技术的研究进展[J],自然科学进展,2002,12(1):13～19
168 杨炜,毕学知,黄大年等,水稻蛋白质的双向电泳分析技术,中国水稻科学,1993, 7(1):43～47
169 余志良,杨晟,蔡谨,袁中一,S-腺苷甲硫氨酸研究进展,中国医药工业杂志,2003,34(1):49～52
170 钟伯雄,水稻蛋白质双向电泳分析新方法,浙江农业大学学报,1997,23(2):128～132
171 朱宏,马旭俊,马兴宏,王同昌,小麦叶片蛋白质的双向电泳分析,哈尔滨师范大学自然科学学报,2001,17(1):98～101
172 朱友林,王建,余潮,植物蛋白质双向电泳及其考马斯亮兰-银染复合染色法,南昌大学学报,1999,23(2):101～105
173 朱友林,吴健胜,王金生,水稻对白叶枯病菌抗性相关蛋白的双向电泳分析,中国农业科学,2000,33(4):91～93
174 邹辉琼,聚丙烯酰胺凝胶中蛋白质快速染色的改良法,生物化学与生物物理进展,1989 ,16(5):401
175郝再彬,苍晶,孙鑫,水稻无侧根突变体的根向重力性异常,植物生理与分子生物学学报,2002,28(3):205～208
176 郝再彬,一井真比古.无侧根及び重力屈性异常を示すイネ突然变异体RM109の形态的特性及び遗传解析,日本作物纪事,1999,68(2):245～252
177 郝再彬,苍晶,徐仲,高继国,一井真比古,水稻侧根突变体RM109的农艺性状.东北农业大学学报,2001,32(1):75～79
178 一井真比古.植物の根に关する诸问题,—突然变异体による遗传解析—,农业及び园芸,1994,69,1223～1237
179 梁正伟,一井真比古.イネ品种IR8由来短根突然变异体LM10幼植物形态特性,日本作物纪事,1996,65,473～478
180 郝再彬,苍晶,孙鑫,水稻无侧根突变体RM109的氧化还原代谢, 核农学报,2002,16 (4) :193～196
2 Anders M Lindroth,Peter Saarikoski, Gunnar Flygh,etal. Two S-adenosylethionine synthetseencoding genes differentially expressed duringadventitious root development in Pinus contor- ta. Plant Molecular Biology ,2001,46:335～346
3 Anderson NG,Anderson NL,Twenty years of two-dimensional electrophoresis :Past, present and future[J],Electrophoresis,1996,17(3):443～453
4 Anderson NL,Anderson NG,Proteome and prteomics:new technologies,new concepts, and new words,Electrophoresis,1998,19:1853～1861
5 Bcerjan W, Cervera M T ,Delarue M, Beeck man T,Superroot,a recessive mution in Arabid opsis,confers ausin overproduction ,Plant cell,1979,7:1405～1419
6 Beeck man T, Burssens S, Inze D. The peri-cell-cycle in Arabidopsis. Journal of Experim- ental Botany, 2001,52:403～411
7 Bell J K,McCully M G. Ahistological study of lateral root initiation,development in Zea mays.Protoplasma,1970,70:179～205
8 Beyer K, Bardina L, Grishina G, Sampson HA.,Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: seed storage proteins as common food allergens,J Allergy Clin Immunol, 2002, 110(1):154～159
9 Biermann B, Feldman LJ.,Physiological, biochemical and molecular processes associated with gravitropism in roots of maize, Adv Space Res,1994:14(8):331～340
10 Bjellqvist B, Sanchez JC, Hochstrasser DF, etal. Micropreparative two dimensional electro- phoresis allowing the separation of samples containing milligramamounts of proteins, Elec- trophoresis,1993,14:1375～1378
11 Blakely L M,Durham m,e vans T A,Blakely R M.Experimental studies on lateral root form- ation in radish seeding roots.General metheds,developmental stages, spontaneous formation of laterals.Bot Gaz, ,1982,143(3):341～352
12 Blakely L M,Evans T A, Cell dynamics studies on the pericycle of radish seeds roots,Plant Sci Lett,1982,14:79～83
13 Carlos M. Vicient, Natacha Bies-Etheve and Michel Delseny ,Changes in gene expression in the leafy cotyledon1 (lec1) and fusca3 (fus3) mutants of Arabidopsis thaliana L, Journal of Experimental Botany, 2000,347:995～1003
14 Casero P J,Casimiro T, Rodnguez-Callardo L,Martin-Partido G,Lateral root initation by means of a transversal divisions of the pericycle cells in adventitioous roots of Alliumcepa , Protoplasma,1993,176:138～144
15 Casero P J,Casimiro T,Loret P G,Later root initiation by means of asymmetrical transversal divisions of the percycle cells in four different species of plants:Raphanus sativus, Helianth- us annuns, Zea mays,Daucus carota, Protoplasma,1995,188:49～58
16 Casimiro T, Marchant A, Bhalerao R P, Beeck man T,Dhooge S, Auxin transport promotes Arabidopsis lateral root initiation,Plant Cell,2001,13:843～852
17 Castellanos Serra LR, Fernandez Patron C, Huerta V,etal.A procedure for protein elution from reverse stained polyacrylamide gels applicable at the low picolmole level:analternative route to the preparation of low abundance proteins formicroanalysis. Electrophoresis, 1996, 17:1564～1572
18 Celenza J,Grisafi P L,Fink G R, A pathway for lateral root formation in Arabidopsis thaliana,Gene Dev,1995,9:2131～2142
19 Celis J E,Gromov P.2-D protein electrophoresis :Canitbe perfected [J] Curr Opin Biotechnol ,1999,10(1):16～21
20 Chait BT, Wang R, KentS BH,etal. Protein ladder sequencing.Science,1993,62:89～92
21 Chambers G,Lawrie L,Cash P,Murray G I.Proteomics:A new approach to the study of disease[J].J Pathol,2000,192(3):280～288
22 Cheng J C ,Seeley K A, Sung Z A , Arabidopsis genes required for cell proliferation at the root tip ,Plant Physiol,107 :365～376
23 Chevallet M, Santoni V, Rabilloud T, etal. Newzwitter ionic detergents improve the analysis of membrane proteins by two dimensional electrophoresis. Electrophoresis, 1998,19:1901～1909
24 Christine Finnie,Sabrina Melchior,Peter Roepstorff, Birte Svensson,Proteome Analysis of Grain Filling and Seed Maturation in Barley,Plant Physiol, 2002, 129 : 1308～1319
25 Constance N, White,Carol J, Rivinn,Gibberellins and Seed Development in Maize, Gibb- erellin Synthesis Inhibition Enhances Abscisic Acid Signaling in Cultured Embryos. Plant Physiol, 2000, 122:1089～1098
26 Constance N. White,William M. Proebsting, Peter Hedden, Gibberellins and Seed Development in Maize. I.?Evidence That Gibberellin/Abscisic Acid Balance Governs Germination versus Maturation Pathways,April Plant Physiol,2000, (122):1081～1088
27 Cremer F,Van de Wallec. Methods for Extraction of Proteins from Green Plant Tissues for Two-Dimensional, Polyacrylamide Gel Electrophoresis.Anal Biochem,1985,147: 22～26
28 Dcerner P, Cell cycle regulation in plants ,Plant Physiol,1994,106:823～827
29 Dcerner P, J rgensen J E,You R,Steppuhn J,Control of root growth,development by cyclin expression ,Nature ,1996,380:520～523
30 Di Layrenzio L,Wysocka-Diller J,Malamy J E,Pysh L,Helariutta Y,Freshour G,Hahn M G,The SCARECROW gene regulates an asymmetric cell division that is essential for generating the radial organization of the Arabidopsis root,Cell,1996,423～433
31 Dittmer H J,A quantitative study of the roots, root hairs of a winter rye plant ,Amer J Bot, 1987,24:417～420
32 Dolan L,Janmaat K,Willemsen V,Linstead P, Pcethig S,Roberts K,ScheresB,Cellular organ- isation of the thaliana root ,Development,1993,119:71～84
33 Dominique Van Der Straeten,Renato A Rodrigues-Pousada,Howard M.Goodman, etal Plant Enclose:Gene structure,Expression,and Evolution,Plant Cell, 1991, 3 : 719～735
34 Dubrovsky J G,Dcerner P W,Colon-Carmona A, Rost T L,Pericycle cell proliferation , lateral root initation in Arabidopsis Plant Physiol ,2000,124:1648～1657
35 Ferreira P C G, Hemerly A S, de Almeida, Van Montagu M, Engler G, Developmental expression of the Arabidopsis cyclin gene cyc1At,Plant Cell,1996,6:1763～1774
36 Figeys D, Aebersold R. High sensitivity identification of proteins by electrospray ionization tandem mass spectrometry: initial comparison between an ion trap mass spectrometer and a triple quadrupole mass spectrometer. Electrophoresis, 1997,18: 300～368
37 Figeys D,McBroom L D,Moran M F.Mass spectrometry for the study of protein interactions [J],Methods,2001,24(3)230～239
38 Frank Hochholdinger, Woong June Park, Günter H. Feix. Cooperative Action of SLR1 and SLR2 Is Required for Lateral Root-Specific Cell Elongation in Maize. Plant Physiol, 2001, 125:1529～1539
39 Frank Hochholdinger,GunteFeix.Early post-embryonic root formation is specifically affected in the maize mutant lrt1.1998,Plant Journal,16(2):247～255.
40 Fukaki H,Fujisawa H,Tasaka M,SGR1.SGR2,SGR3:novel genetic loci involved in shoot gravitropism in Arabidopsis ,Plant Physiol,1996,100:945～955
41 Fukali H,Wysocka-Diller J W,Kato T,Fujisawa H,Benfey P N,Tasaka M,Genetic evidence that endodermis is essential for shoot gravitropism in Arabidopsis thaliana, Plant J,1998, 14:425～430
42 Gaskell SJ. Electrospray: principles and practice. Journal of Mass Spectrometry, 1997,32: 677～688
43 Gorg A, Obermaier C, Madjar JJ. Very alkaline immobilized pH gradients for two dimensional electrophoresis of ribosomal and nuclear proteins, Electrophoresis, 1997,18:328～337
44 Gorg A, Postel W,Gunther S.The current state of two dimensional electophoresis with inmobilized pH gradient.Electrophoresis,1988,9:531～546
45 Gorg A,Obermaier C,Boguth G,Harder A,Scheibe B,Wild-gruber R,Weiss W. The current state of two-dimensional electrophoresis with immobilized pH gradients [J]. Electrophore- sis,200 ,21(6):1037～1053
46 Griffin T J, Goodlett D R, Aebersold R, Advances in proteome analysis by mass spectrometry [J].Curr Opin Biotechnol,2001,12(6):607～612
47 Gygi SP,Corthals GL,Zhang Y,Rochon Y,Aebersold R.E-valuation of two- dimensional gel electrophoresis-based proteome analysis technology[J].Proc Natl Acad Sci USA, 2000, 97 (17) :9390～9395.
48 HaoZai-bin, LanXiao-xia, YangLiang-qun. Plant Hormone Resistance and Agronomic Characteristics of the MT10 Mutantin Rice, Journal of Northeast Agricultural University 2000,7 (2):130～135
49 HaoZB,XuZH,CangJ,IchiiM.MT10 Mutant of Rice with Altered Lateral Root Initiation. Journal of Northeast Agricultural University,2000,7(7):38～42
50 Haynes PA, Gygi SP, Aebersold R, etal. Proteome analysis:biological assay or data archive Electrophoresis,1998,19:1862～1871
51 Heck GR, Chamberlain AK, Ho TH. Barley embryo globulin 1 gene, Beg1: character- ization of cDNA, chromosome mapping and regulation of expression. May; Mol Gen Genet, 1993, 239(1-2):209～218
52 Hemerly A S,Ferveira P,de Almeida,Engler G,lnze D,Cdc2a experession in Arabidopsis is linked with competence for cell dvision ,Plant Cell,1993,5:1711～1723
53 Henzel WJ, Billeci TM, Watanae C, etal. Identifying proteins from two dimensional gels
by molecular mass searching of peptide fragments in protein sequence database, Proc Natl Acad Sci USA,1993,90:5011～5015
54 Hidehiko Kikuchi,Sakiko Hirose, Seiichi Toki,etal. Molecular characterization of a gene for alanine aminotransferase from rice, Plant Molecular Biology,1999, 39: 149～159.
55 Hirano H.Microsequence analysis of winged bean seed proteins electroblotted from two-dimensional gel,J Protein Chem, 1989 ,8(1):115～130.
56 Hobbie L,Estelle M,The axr4 auxin-resistant mutants of Arabidopsis thaliana define a gene important for root gravitropism,lateral root initiation , Plant J,1995,7:211～220
57 Hochholdinger F,Park W J,Feix G H,Cooperative action of SLR1,SLR2 is required for lateral root–specific cell elongation in maize,Plant Physiol,2001,125:1529～1539
58 Holly Hilton,Brandon S,Gaut,Speciation and Domestication in Maize and Its Wild Relatives: Evidence From the Globulin-1 Gene. Genetics, 1998, 150:863～872
59 Humphery Smith I,Cordwell SJ,Proteom research:comple mentarity and limitations respect to the RNA and DNA worlds,Electrophoresis,1997,18:1217～1242
60 John W.Schiefelbein,Philip N.Benfey,The Development of Plant Roots:New Approa- ches to Undergroud Problems, Plant Cell, 1991, 3:1147～1154
61 Jungblut P,Thiede B,Protein identification from 2-DE gels by MALDI mass spectr- ometry. Mass Spectrometry reviews,1997,16:145～162
62 Keller B,Lamb CJ,Specific wxpression of a novel cell wall hydroxyproline–rich glycoprotein in lateral root initiation, Genes Dev, 1989,3:1639～1649
63 Karine Gallardo,Claudette Job,Steven P.C.Groot,etal.Proteomics of Arabidopsis Seed Germination.AComparative Study of Wild-Type and Gibberellin-Deficient Seeds, Plant Physiol 2002,129:823～837
64 Karlowski W M, Hirsch A M,The over-expression of an alfalfa RING-H2 gene induces pleiotropic effects on plant growth and development,Plant Mol Biol, 2003, 52(1): 121～133
65 Konishi M, Sugiyama M.,Genetic analysis of adventitious root formation with a novel series of temperature-sensitive mutants of Arabidopsis thaliana, Development, 2003, 130 (23):5637～5647
66 Jocelyn E. Malamy, Katherine S. Ryan ,Environmental Regulation of Lateral Root Initiation in Arabidopsis,Plant Physiol, 2001,127: 899～909
67 Kikuchi H, Hirose S, Toki S, Akama K, Takaiwa F,Molecular characterization of a gene for alanine aminotransferase from rice (Oryza sativa),Plant Mol Biol,1999, 39(1):149～159
68 Van der Straeten D, Rodrigues-Pousada RA, Goodman HM, Van Montagu M.,Plant enolase: gene structure, expression, and evolution.Plant Cell,1991,3(7):719～735
69 Kikuchi H., Hirose S., Toki S , Akama,K, Takaiwa F,Molecular characterization of a gene for alanine aminotransferase from rice (Oryza sativa), Plant Mol. Biol, 1999, 39 (1): 149～159
70 King J J,Stimart D P, Fisher R H,Bleecker A B,A mutation altering auxin homeostasis, Plant morpho;ogy in a Arabidopsis,Plant Cell,1995,7:2023～2037
71 Klee J H,The effects of overproduction of two Agrobacteriumtumefaciens T-DNA auxin
biosynthetic gene products in transgenic petunia plants ,Genes Dev ,1987,1:86～96
72 Klose J,Kobalz U,Two dimensional electrophoresis of proteins:an updated protocol and implications for a functional analysis of the genome, Electrophoresis,1995, 16: 1034～1059
73 Lal SK, Johnson S, Conway T, Kelley PM.Characterization of a maize cDNA that complements an enolase-deficient mutant of Escherichia coli. May, Plant Mol Biol, 1991, 16 (5) :787～795
74 Larkin P J, Gibson J M, Mathesius U, Weinman J J, etal.Transgenic white clover. Studies with the auxin-responsive promoter, GH3, in root gravitropism and lateral root development. Transgenic Res,1996,5(5):325～335
75 Tsutomu Suzuki, Maki Terasaki, Chie Takemoto-Hori,etal.Proteomic Analysis of the Mam- malian Mitochondrial Ribosome. J. Biol. Chem., 2001, 276,(35):33181～33195
76 Liu W, Xu ZH, Luo D, Xue HW.,Roles of OsCKI1, a rice casein kinase I, in root developm- ent and plant hormone sensitivity,Plant J,2003 ,36(2):189～202
77 Laskowski M J,Williams M E,Nusbaum H C,Sussex T M,Formation of laeral root meristems is a two-stage process ,Development ,1995,121:3303～3310
78 Latter GI, Burbeck S,Leavitt J,etal. Identification of polypep tides on two dimensional electrophoresis gels by aminoacid comparison.ClinChem,1984,30:1925～1932
79 Lien B. Lai, S. Lorraine Tausta, and Timothy M. Nelson .Differential Regulation of Transcripts Encoding Cytosolic NADP-Malic Enzyme in C3 and C4 Flaveria Species, Plant Physiol, 2002, 128:. 140～149
80 Liepman AH, Olsen LJ.,Alanine aminotransferase homologs catalyze the glutamate: Glyoxylate aminotransferase reaction in peroxisomes of Arabidopsis, Plant Physiol, 2003,131 (1) :215～227
81 Lincoln,C.,J.H.Britton,M.Estelle.Growth and development of the axr1 mutants of Arabidopsis.PlanntCell, 1990 (2):1071～1080
82 Link AJ.Auto radio graphy of 2-D gels[J],Methods Mol Biol,1999,112:285～290
83 Litts J C, Colwell G W, Chakerian R L, Quatrano R S,The nucleotide sequence of a cDNA clone encoding the wheat Em protein, Nucleic Acids Res, 1987,15(8):3607～3618
84 Nambara E, Hayama R, Tsuchiya Y, Nishimura M,etal.The role of ABI3 and FUS3 loci in Arabidopsis thaliana on phase transition from late embryo development to germination. Dev Biol. 2000, 220(2):412～423
85 Lloret P G,Casero P J,Puglann A,Navascues J,The behavious of two cell populations in the pericycle of Alliumcepa ,Pisum sativum,Daucus carota during early lateral root developme- nt,Ann Bot,63:465～475
86 LooJ A,DeJohn DE,Du P,Stevenson TI,OgorzalekLoo RR,Application of mass spectro- metry for target identification and characterization [J]. Med Res Rev,1999, 19 (4):307～319
87 Lourdes Gomez-Gomez, Pedro Carrasco. Differential Expression of the Saenosyi Lmethionine Synthase Genes during Pea Development, Plant Journal, 1998,117: 397～ 405
88 Lourdes Gómez-Gómez,Pedro Carrasco. Differential Expression of the S-Adenosyl-L- Methionine Synthase Genes during Pea Development,Plant Physiol, 1998, 117: 397～405
89 Luerssen H, Kirik V, Herrmann P, Misera S.FUSCA3 encodes a protein with a conserved VP1/AB13-like B3 domain which is of functional importance for the regulation of seed maturation in Arabidopsis thaliana, Plant J, 1998, 15(6):755～764
90 Lyford W H,Rhizography of non-woody roots of trees in the forest floor,In:Torrey J,The deve- lopment ,function of roots,New York:Academic Press:179～196
91 Malamy J E,Benfey P N,Organization,cell differentiation in lateral roots of Arabid- opsis thaliana,Development,1997,124:33～44
92 MalamyJ E, Benfey P N,Down,out in Arabidopsis:the formation of lateral roots,Trends Plant Sci,1997,2(10):390～396
93 Mann M,Hendrickson RC,Pandey A.Analysis of proteins and proteomes by mass spectrometry[J].Annu Rev Biochem,2001,70:437～473
95 Markus L, Goddemeier , Günter Feix,Genomic structure of the maize TATA-box binding protein 1 (TBP-1): conserved exon/intron structure in eukaryotic TBP genes, 1996 ,26: 111～114
96 Meyerowitz E M,Prehistory,history of Arabidopsis research ,Plant Physiol, 2001,125: 15～19
97 Muday G K,Haworth P,Tomato root growth,gravitropism,lateral development: coorela- tion with auxin transport,Plant Physiol Biochem,1994,32:193～203
98 Muench D G, Good A G.,Hypoxically inducible barley alanine aminotransferase: cDNA cloning and expression analysis,Plant Mol Biol,1994,24(3):417～427
99 Mullner S,Neumann T,Lottspeich F,Proteomics—a new way for drugtarget discovery, Arzneim Forsch/Drug Res,1998,48(1):93～95
100 O’ Farrel P H. High-resolution two–dmensional electrophoresis of proteins, J Biol Chem,1975, 250: 4007～4021
101 Ong SE,Pandey AB.An evaluation of the use of two-dimensional gel electrophor- esis in proteomics[J],Biomol Eng,2001,18(5):195～205
102 Pandey A,Mann M.Proteomics to study genes and genomes[J], Nature,2000, 405 (6788):837～846
103 Proteomic Analysis of the Mammalian Mitochondrial Ribosome, Biol. Chem. 2001,276(35) 33181～33195
104 Qiaoping Yuan, Feng Liang, Joseph Hsiao,etal. Anchoring of rice BAC clones to the rice genetic map in?silico, Nucleic Acids Research, 2000, 28(18): 3636～3641
105 Rabbilloud T,AdessiC,LunardiJ,etal.Improvement of the solubilization of proteins in two dimensional electrophoresis with immobilised pH gradients.Electrophoresis,1997, 18:307～316
106 Rabbilloud T. Use of thiourea to increase the solubility of membrane proteins in two dimensional electrophoresis. Electrophoresis,1998,19:758～760
107 Rabilloud T, Valette C, Lawrence JJ. Sample application by in gel rehydration improves the resolution of two dimensional electrophoresis with immobilized pH gradients in the
first dimension. Electrophoresis,1994,15:1552～1558
108 Rabilloud T.Silver staining of 2-D electrophoresis gels [J]. Methods Mol Biol, 1999,112:297～305
109 Rashotte A M,Brady S R,Reed R C,Ante S J,Muday G K,Basipetal auxin transport is required for gravitropism in roots of Arabidopsis ,Plant Physiol,2000,122:481～491
110 Reed R C,Brady S R,Muday G K,Inhibition of auxin movement from the shoot into inhibits lateral root development in Arabidopsis,Plant Physiol,1998,118:1369～1378
111 Rodrigues-Pousada R A, De Rycke R, Dedonder A, etal, The Arabidopsis 1-Amino cyclopro pane-1-Carboxylate Synthase Gene 1 Is Expressed during Early Development, Plant Cell. 1993,5(8):897～911
112 Rogg L E,Lasswell J,Bartel B,A gain-of-function mutation in IAA28 suppresses lateral root development ,Plant Cell,2001,13:465～480
113 Ruegger M,Dewey E,Hobbie L,Brown D,Bernasconi P,Turner J,Muday G,Estelle M,Reduced naphthylphthalamic acid binding in the tir3 mutant of Arabidopsis is associated with a reduction in polar auxin transport, diverse mor phological defects, Plant cell,1997,9:745～757
114 Sanchez JC, Appel RD, Hochstrasser DF. Inside SWISS 2-D PAGE database, Electrophoresis,1995,16:1131～1151
115 Scheres B, Mc Kahnn H I,Van den Berg C, Roots redefined: anatomical, genetic analysis of root development, Plant Physio,1999,111:959～964
116 Shailesh K. Lal, Chwenfang Lee, and Martin M. Sachs.Differential Regulation of Enolase during Anaerobiosis in Maize, Plant Physiol,1998,118: 1285-1293
117 Sharon Rozovsky, Gerwald Jogl, Liang Tong, Ann E. McDermott,Solution-state NMR investigations of triosephosphate isomerase active site loop motion: ligand release in relation to active site loop dynamics,Journal of Molecular Biology, 2001,310,(1) 271～280
118 Shevchenkenko A, Jensen ON,Mann M, etal. Linking genome and proteome by mass spectrometry large scale identification of yeast proteins form two dimensional gels. Proc Natl Acad Sci USA,1996,93:14440～14445
119 Smith D L,Fedoroff N V,LRPI,a gene expressed in lateral, adventitious primodia of Arabido psis,Plant Cell,1995,7:735～745
120 Takahiro Adachi,Hidehiko Izumi,Takehisa Yamada,etal.Gene structure and expression of rice seed allergenic proteins belonging to the α-amylase/trypsin inhibitor family, 1993, Plant Molecular Biology 21:239～248
121 Thomas C, Meyer D, Wolff M, Himber C, Alioua M, Steinmetz A.Molecular character- ization and spatial expression of the sunflower ABP1 gene,Plant Mol Biol,2003, 52 (5): 1025～1036
122 Timpte C,Lincoin C,Pickett F B,Turner J,Estelle M,The AXRI,AVXI genes of Arabid- opsis function inseparate auxin-response pathways,Plant J,1995,8:561～569
123 Timpte,C.,A.K.WilsonandM.Estelle.Theaxr2-1mutation of Arabidopsis thalianain a gain of function mutation that disruptsa nearly step inauxin response.Genetics, 1994, (138):1239～1249
124 Tonella L, Walsh BJ, Hochstrasser DF, etal.’98 Escherichia coli SWISS 2-D PAGE database update.Electrophoresis,1998,19:1960～1971
125 Torrey J G,The induction of lateral roots by indole acetic acid, root decaitation,Am J Bot, 1950,37:257～264
126 Traini M,Gooley AA, Williams LK, etal.Towards an automated approach for protein in identification proteome projects. Electrophoresis,1998,19:1941～1949
127 Tsuji H, Hiemori M, Kimoto M,etal. Cloning of cDNA encoding a soybean allergen, Gly m Bd 28K,Biochim Biophys Acta,2001,19;1518(1-2):178～182
128 Urquhart BL,Atsalos TE,Humphery Smith I,etal.Proteomic contigs of Mycobacterium tube rculosis and mycobacterium bovis(BCG) using novel immobilised pH gradients. Electrophoresis,1997,18:1384～1392
129 Van de Sande K,Modification of phytohormone response by a peptide encoded by ENOD40 of legumes,a nonlegume,Science,1996,273:370～373
130 Vernoux T,Wilson R C,Seeley K A,Reichheld J P,Muroy S,Brown S,Maughan S C, Cobbett C S,The ROOT MERISTEMLESS1/CADMIUM SENSITIVE 2GENE defines a glutathione dependent pathway involved in initiation,maintenance of cell division during postembryo- nic root development,Plant Cell,2000,12:97～109
131 Wang S C, Ichii M,Taketa S,Xu L L,Xia K,Zhou X,2002,Effect of jasmonic acid on lateral root formation in rice seedling. Acta Botanca Sinica,44(4):502～504
132 Wang S C,Ichii M,Taketa S,Xu L L,Xia K, Zhou X,2002b.Lateral root formation in rice SEEDLING:promotion effect of jasmonic acid.Journal of Plant Physionlogy, 159: 827～832
133 Wang Shu-Cai,Masshiko Ichii,Shin Taketa. Effect of Jasmonic Acid on Lateral Root Formation in Rice seedling,Acta Botanica Sinica, 2002,44(4):502～504
134 Waterborg JH,Matthews HR,Fluorography of polyacryl amide gels containing tritium[J],Methods Mol Biol,1994,32:163～167
135 Wightman F, Thimann K V,Hormonal factors controlling the initiation,development of lateral roots,Source of primodiuminducing substances in the primary root of pea seedings,Physiol Plant,49:13～20
136 Wilkins M R,Sanchez J C,Williama K L, Progress with proteome projects: Why allproteins expressed by agenome should bei dentified and how to do it ,Biotechnology and Genetic Engineering Reviews,1995,13:19～45
137 Willemsen V,Wolkenfelt H,de Vrieze G,Weisbeek P,Scheres B,The HOBBIT gene is required for formation of root meristem in the Arabidopsis embyryo, Development, 1998,125:521～531
138 Winfried Hetz,Frank Hochholdinger,Michael Schwall,Gunter Feix. Isolation and characterization of rtcs,a maize mutant deficient in the formation of nodal roots, Plant Journal, 1996,10(5):845～857
139 Wirth PJ,Hoang TN, Benjamin T. Micropreparative immobilezed pH gradient two dimensional electrophoresis incombination with protein microsequecing for the analysis of humanliver proteins. Electrophoresis,1995,16:1946～1960
140 Wu J., Cramer C.,Hatzios K K.,Isolation of a full-length cDNA encoding the second glutathione S-transferase from rice ,Plant Physiol,1998,118 (1), 329
141 Wysocka-Diller J W,Helariutta Y,Fukaki H,Malamy J E,Benfey P N,Molecular analysis of SCARECROW function reveals a radial patterning mechanism common to root, shoot. Development,2000,127:595～603
142 Xu Y, Hall,T C ,Cytosolic triosephosphate isomerase is a single gene in rice,Plant Physiol, 1993, 101 (20):683～687
143 Xu Y, Hall,T C, The induction of lateral roots by indole acetic acid, root decaitation,Am J Bot, 1950,37:257～264
144 Xu,Y,Harris-HallerL.W.,McCollum J.C.,etal.Nuclear gene encoding Cytosolic Trio- sephosphate isomerase from rice.1993,Plant Physiol. 102 (2): 697
145 Yates JR. Mass spectrometry and the age of the Proteome. Journal of mass spectro- metry, 1998,33:1921～1930
146 Yong Xu,Timothy C,Hall.Cytosolic Triosephosphate Isomerase Is a Single Gene in rice, Plant Physiol, 1993,101:683～687
147 成海平,钱小红,蛋白质组研究的技术体系及其进展,生物化学和生物物理进展, 2000, 27(6):584～588
148樊跃平,于健春,余跃,张琳,谷胱甘肽的生理意义及其各种测定方法比较、评价,中国临床营养杂志,2003,11(3):136～139
149 高原,王国秀,刘中来,一种经济、简便的双向电泳方法,生物化学与生物物理进展,2003,30(1):156～158
150 谷瑞升,刘群录,陈雪梅,蒋湘宁,一种省时高效的木本植物蛋白双向电泳分析方法,北京林业大学学报,1999,21(5):7～10
151 胡金勇,曾英,桑玉英,双向电泳分析鸢尾绿白嵌合叶片的蛋白质,云南植物研究。 2002,24 ( 3) : 387～391
152 黄英金,熊伟,漆映雪,刘宜柏,稻米蛋白质及其4种组分含量的遗传研究,江西农业大学学报,2000,22(4):473～478
153 贾宇峰,林秋霞,郭尧君,等.蛋白质双向电泳图像分析[J].生物化学与生物物理进展, 2001,28(2):246～250
154 李伯良,李林,吴家睿.功能蛋白质组学[J].生物工程进展,1999,19(4):15～16.
155 李林,吴家睿,李伯良.蛋白质组学的产生及其重要意义[J].生命科学,1999,11(2): 49～50
156 李林.蛋白质组学的进展[J].生物化学与生物物理学报,2000,27(3):227～231.
157梁五生,毛碧增,李帅,庄芳蕾,李德葆,水稻种子萌发过程中氰丙氨酸合酶的活性变化与分布,植物生理学报, 2001,27(3):243～247
158 廖祥儒,刘小丽,陈彤,袁京云,李业英,王俊丽,杜建芳,谷胱甘肽对小麦幼穗胚性愈伤组织形成以及几种相关酶活性的影响,植物生理学通讯,2002,38(4):327～329
159 逯斌,林兵.一种改良的植物蛋白质双向电泳方法.生物化学与生物物理进展,1989, 16(6):480～481
160 毛君铃,从双向电泳到生物质谱—蛋白质组分析技术的最新进展,现代科学仪器2001,2:37～38
161 戚元成,张慧,赵彦修,植物谷胱甘肽转移酶和盐胁迫,山东师范大学学报(自然科学版),2002,17(2):71～75
162 阮禹松,赵文明,种子盐溶球蛋白的结构特征 ,西北植物学报,2002,22(4):999～1003
163 万晶宏,贺福初.蛋白质组技术的研究进展,科学通报,1999,44(9):904～910
164 王台.一种适用于双向电泳凝胶的染色方法.植物学通报,1996,13(4):56～57
165 王新,王伯良,张宗友,等.蛋白质组分析中双向电泳技术的改良和应用,细胞与分子免疫学杂志,2001,17(2):197～199
166 吴庆如,黄开勋,徐辉碧,双向凝胶电泳技术进展[J],生物技术通讯,2002,13(2): 28～31
167 杨何义,应万涛,钱小红,蛋白质组技术的研究进展[J],自然科学进展,2002,12(1):13～19
168 杨炜,毕学知,黄大年等,水稻蛋白质的双向电泳分析技术,中国水稻科学,1993, 7(1):43～47
169 余志良,杨晟,蔡谨,袁中一,S-腺苷甲硫氨酸研究进展,中国医药工业杂志,2003,34(1):49～52
170 钟伯雄,水稻蛋白质双向电泳分析新方法,浙江农业大学学报,1997,23(2):128～132
171 朱宏,马旭俊,马兴宏,王同昌,小麦叶片蛋白质的双向电泳分析,哈尔滨师范大学自然科学学报,2001,17(1):98～101
172 朱友林,王建,余潮,植物蛋白质双向电泳及其考马斯亮兰-银染复合染色法,南昌大学学报,1999,23(2):101～105
173 朱友林,吴健胜,王金生,水稻对白叶枯病菌抗性相关蛋白的双向电泳分析,中国农业科学,2000,33(4):91～93
174 邹辉琼,聚丙烯酰胺凝胶中蛋白质快速染色的改良法,生物化学与生物物理进展,1989 ,16(5):401
175郝再彬,苍晶,孙鑫,水稻无侧根突变体的根向重力性异常,植物生理与分子生物学学报,2002,28(3):205～208
176 郝再彬,一井真比古.无侧根及び重力屈性异常を示すイネ突然变异体RM109の形态的特性及び遗传解析,日本作物纪事,1999,68(2):245～252
177 郝再彬,苍晶,徐仲,高继国,一井真比古,水稻侧根突变体RM109的农艺性状.东北农业大学学报,2001,32(1):75～79
178 一井真比古.植物の根に关する诸问题,—突然变异体による遗传解析—,农业及び园芸,1994,69,1223～1237
179 梁正伟,一井真比古.イネ品种IR8由来短根突然变异体LM10幼植物形态特性,日本作物纪事,1996,65,473～478
180 郝再彬,苍晶,孙鑫,水稻无侧根突变体RM109的氧化还原代谢, 核农学报,2002,16 (4) :193～196