MyoG、Myf-5基因在申农1号品系猪中的多态性及其与肉质相关性的研究
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摘要
本文采用PCR-RFLP分析方法，对33头申农Ⅰ号品系育肥猪进行了MyoG基因和Myf-5基因的研究，分析了各基因型之间在初生重、断奶重、系水力、剪切力、肌内脂肪及瘦肉率等性状间的差异。从而找出MyoG基因和Myf-5基因与生长性状和肉质性状之间的相关性，尝试为猪的育种工作提供分子标记。
研究结果表明，MyoG基因和Myf-5基因都存在多态性。MyoG基因用PCR1 Msp Ⅰ-RFLP法检测，表现为两种基因型，AA型和AB型；MyoG基因用PCR1 Msp Ⅰ-RFLP法检测，表现为三种基因型，MM型、MN型和NN型；Myf-5基因用PCR-Hinf Ⅰ-RFLP法检测，表现为两种基因型，EE型和EF型。通过SAS分析，表明申农Ⅰ号品系猪的MyoG基因的PCR-Msp Ⅰ-RFLP位点与猪的初生重、系水力相关(p＜0.05)，与断奶重、剪切力及肌内脂的不相关(p＞0.05)。申农Ⅰ号品系猪的Myf-5基因的PCR-Hinf Ⅰ-RFLP位点与猪的初生重、肌内脂相关(p＜0.05)。
Polymorphisms of MyoG gene and Myf-5 gene in Shennong No.I pig line were studied in the experiment, by means of PCR-RFLP. The differences of birth weight, weaning weight, water holding capacity, shear force, intratnusclar fat and carcass lean percentage among different genotypes and the polymorphisms of MyoG gene and Myf-5 gene were analysed. The correlation bewteen the two genes and growth performance with meat quality would be found out to identify a molecular marker for pig breeding.
The results showed that there were polymorphisms of MyoG gene and myf-5 gene in Shennong No.I pig line. The genotypes of MyoG gene PCR1 Msp I-RFLP have AA and AB; MyoG gene PCR2 Msp I -RFLP have MM, MN and NN, and Myf-5 gene PCR Hinf I -RFLP have EE and EF. Polymorphisms of MyoG gene were correlated with birth weight and water holding capacity in Shennong No.I pig (p<0.05), and polymorphisms of Myf-5 gene were correlated with birth weight and intramuscular fat(p<0.05).
研究结果表明，MyoG基因和Myf-5基因都存在多态性。MyoG基因用PCR1 Msp Ⅰ-RFLP法检测，表现为两种基因型，AA型和AB型；MyoG基因用PCR1 Msp Ⅰ-RFLP法检测，表现为三种基因型，MM型、MN型和NN型；Myf-5基因用PCR-Hinf Ⅰ-RFLP法检测，表现为两种基因型，EE型和EF型。通过SAS分析，表明申农Ⅰ号品系猪的MyoG基因的PCR-Msp Ⅰ-RFLP位点与猪的初生重、系水力相关(p＜0.05)，与断奶重、剪切力及肌内脂的不相关(p＞0.05)。申农Ⅰ号品系猪的Myf-5基因的PCR-Hinf Ⅰ-RFLP位点与猪的初生重、肌内脂相关(p＜0.05)。
Polymorphisms of MyoG gene and Myf-5 gene in Shennong No.I pig line were studied in the experiment, by means of PCR-RFLP. The differences of birth weight, weaning weight, water holding capacity, shear force, intratnusclar fat and carcass lean percentage among different genotypes and the polymorphisms of MyoG gene and Myf-5 gene were analysed. The correlation bewteen the two genes and growth performance with meat quality would be found out to identify a molecular marker for pig breeding.
The results showed that there were polymorphisms of MyoG gene and myf-5 gene in Shennong No.I pig line. The genotypes of MyoG gene PCR1 Msp I-RFLP have AA and AB; MyoG gene PCR2 Msp I -RFLP have MM, MN and NN, and Myf-5 gene PCR Hinf I -RFLP have EE and EF. Polymorphisms of MyoG gene were correlated with birth weight and water holding capacity in Shennong No.I pig (p<0.05), and polymorphisms of Myf-5 gene were correlated with birth weight and intramuscular fat(p<0.05).
引文
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[9] Gao J, Li Z, Paulin D. A novel site, Mt, in the human desmin enhancer is necessary for maximal expression in skeletal muscle. J Biol Chem., 1998, 273(11), 6402
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[12] Firulli A B, et al. Modular regulation of muscle gene transcription: a mechanism for muscle cell diversity. TIG, 1997, 13,364～369
[13] 王东林，毛宝龄，生肌调节因子及其作用机理．国外医学分子生物学分册，1995，17(9)，157～160
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[19] Olson E N, et al. bHLH factors in muscle development: dead lines and commitments, what to
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[20] 王先梅，MyoD家族与分子心肌成形术．国外医学-心血管分册，2001，28(1)，6～9
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[22] Ludoph D C, et al. Transcription factors families: muscling in the myogenic program. The FASEB Journal, 1995, 9, 1595～1604
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[26] 肖国芝等，生肌螺旋-环-螺旋蛋白．生物化学与生物物理进展，1993，20(3)，186～191
[27] 王均等，鸡胚不同发育时期MyoD、生肌素及乙酰胆碱受体基因的表达．北京医科大学学报，1997，29(2)，100～102
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[29] 肖国芝等，鸡AChR γ亚基因远端E盒子与myogenin的相互作用．生物化学杂志，1995，11(5)，516～520
[30] 王先梅等，MyoD家族与分子心肌成形术．国外医学·心血管分册，2001，28(1)，6～9
[31] 王东林等，骨骼肌纤维类型及其细胞与分子基础．国外医学生理、病理与临床分册．1995，15(1)．9～11
[32] Zhang J M, Chen L, Krause M et al. Evolutionary conservation of MyoD function and differential utilization of proteins. Dev Biol., 1999, 208(2), 465
[33] Lattanzi L, Salvatori G, Coletla M, et al. High efficiency myogenic conversion of human fibroblast by adenoviral vector-mediated MyoD gene transfer, An alternative strategy for ex vivo gene therapy of primary myopathies. J Clin Invest. 1998, 101(10), 219
[34] 苏玉红，熊远著，邓昌彦，猪的肉质基因定位研究进展．遗传，2000，22(3)，334～338
[35] 刘伟敏，熊远著，蒋斯文等，猪数量性状位点定位研究进展．湖北农业科学，2000，(2)，55～58
[36] Kitazmann M, Vandromme M, Scheaffer V, et al. edkl and cxlk2-mediatied phosphorylation of MyoD half-life and myogenic activity. Mol Cell Biol. 1999, 19(4), 430
[37] 王先梅，李震一，吴志峰等，乳鼠心肌成纤维细胞MyoD cDNA转染后的形态
变化．第三军医大学学报，2000，22(12)，1148～1150
[38] Sassoon D A, Myogenic regulatory factors: dissecting their role and regulation during vertebrate embryogenesis. Dev Biol, 1993, 156, 11～23
[39] Borycki A G, Li J, Jin F et al. Pax3 functions in cell survival and in pax7 regulation. Development. 1999, 126(8), 1665
[40] Hughes S M, Running without regulators. Nature,1992, 360, 536～537
[41] Tajbakhsh S, et al. Redefining the genetic hierarchies controlling skeletal mygenesis: Pax-3 and myf-5 act upstream of MyoD. Cell, 1997, 89, 127～138
[42] 法永红，顾哓明，周树夏，表情肌肌纤维组织化学分型及分类研究．中华整形外科杂志，2000，16(4)，233～235
[43] Zhang J M, Wei Q, Zhao X et al. Coupling of the cycle and myogensis through the cyclin DI-dependent interaction of MyoD with cdk4. EMBO J. 1999, 18(4), 926
[44] Ewton D Z, Coolican S A, Mohan S et al. Modulation of insulin-like growth factors in L6A1 myoblasts by insulin-like growth factors binding protein (IGFBP)-4 and IGFBP-5: a dual role for IGFBP-5. J Cell Physiol. 1998, 177(1), 47
[45] Nofziger D, Miyamoto A, Lyons K M et al. Notch signaling imposes two distinct blocks in the differentiation of C2C 12 myoblasts. Development. 1999, 126(8), 1689
[46] Balley P, Sartorelli V, Hamamori Y et al. The orphan nuclear receptor, COUP-TF Ⅱ, inhibits myogensis by post-transcriptional regulation of MyoD function: COUP-TF Ⅱ directly interacts with p300 and MyoD. Nucleic-Acids Res. 1998, 26(23), 5501
[47] Melnikova I N, Bounpheng M, Scharteman G C et al. differential biological activities of mammalian Id proteins in muscle cells. Exp cell Res. 1999, 247(1), 94
[48] Lemercier C, To R Q, Carasco R A, et al. The basic helix-loop-helix transcription factor Mistl functions as a transcriptional repressor of MyoD. EMBO J. 1998, 17(5), 1412
[49] Bartb J L, Moroia J, Ivarine H. An Oct like binding factor regulates myf-5 expression in primary avian cells. Exp Cell Res. 1998, 238(2), 430
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