番茄内源激素含量与其耐温度胁迫的关系
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
本实验选择由中国农业大学农学与生物技术学院蔬菜系经过多代自交育成的6-7个番茄品系,在35℃/25℃(d/n)、38℃/28℃(d/n)高温胁迫和12℃、8℃低温胁迫处理下,采用隶属函数法,通过综合评价筛选确定了128、122、125、126、131、137等6个品系的耐高温胁迫能力,128、125、122、133、137、131、126等7个品系的耐低温胁迫能力,并在此基础上对高温、低温胁迫下番茄内源ABA、GA_3、IAA、Z+ZR的含量变化进行了分析,研究了番茄内源激素含量变化与其耐温度胁迫的关系。结果表明:
     (1)高温胁迫研究中采用38℃/28℃(d/n)处理番茄幼苗比较合理。此处理温度对番茄正常生长发育造成的影响在品系间存在显著差异。在对低温胁迫研究中,12℃处理造成番茄生理生化及生长变化在品种间存在显著差异,适合作为研究低温胁迫时采用的处理温度。
     (2)相对于适温25℃/15℃(d/n)条件,高温胁迫下番茄内源ABA、GA_3含量极显著增加,1AA、Z+ZR含量极显著降低。
     (3)ABA在35℃/25℃(d/n)下的相对含量与其耐高温胁迫能力呈显著正相关(r_(ABA)=0.8319~*),Z+ZR在38℃/28℃(d/n)处理下的绝对含量与其耐高温胁迫能力呈极显著负相关(r_(ZR)=-0.9213~(**)),因此ABA在35℃/25℃(d/n)下的相对含量和Z+ZR在38℃/28℃(d/n)下的绝对含量可以作为番茄耐高温胁迫鉴定的指标之一。
     (4)与适温条件下相比较,低温胁迫下番茄内源ABA、Z+ZR含量极显著增加,GA_3含量极显著降低,IAA含量下降不显著。
     (5)番茄内源ABA、GA_3、IAA和Z+ZR含量与其耐低温胁迫能力无显著相关性,不能作为番茄耐低温胁迫的鉴定指标。
     (6)筛选出同时具备良好的耐高温胁迫能力和良好的耐低温胁迫能力的番茄育种材料128号品系。
This dissertation used 6-7 tomato lines from Agronomy and Biotechnology college of CAU, which have been fixed by selfing several generations. The high temperature stress tolerance of lines 128, 122, 125, 126, 131, 137 are evaluated by using the subject function values in 35℃/25℃(d/n) and 38℃/28 ℃(d/n), low temperature stress tolerance of lines 128 , 125, 122, 133, 137, 131, 126 in 12℃ and 8 ℃. Based on the result of the evaluation, the dissertation mainly studied the effect of temperature stress on tomato of the hormones content and their correlation. The main results are as follows:(1) It is good putting tomato plants in 38℃/28℃(d/n) when we study the changes the tomato materials make under heat temperature stress. At the same time, when we studied the cold resistance, 12 ℃will be a good condition to deal with the plants.
    (2) ABA and GA3 contents rise apparently in heat temperature comparatively to the CK temperature (25℃/15℃(d/n) ), IAA and Z+ZR level drop apparently in inverse.
    (3) The correlation between ABA relative content of tomato in 35℃/25℃(d/n) and the heat tolerance is significent, rABA=0-8319*; the correlation between Z+ZR content in 38℃/28℃(d/n) and it's heat tolerance is quite significant, rZR=-0.9213**. The ABA relative content in 35℃/25℃(d/n) and the Z+ZR content in 38℃/28℃ (d/n) can be used to evaluate the heat tolerance of tomato.
    (4) ABA and Z+ZR contents rise apparently in cold temperature comparatively to the CK temperature, GA3 drop apparently, and IAA dosen't drop apparently.
    (5) There is no significent correlation between tomato hormones contents and the cold temperature stress tolerance. The contents of tomato hormones can't be used to evaluat the cold tolerance.
    (6) Get one material (128) having good tolerance both to high temperature and low temperature.
引文
[1]王冬梅,许向阳,李景富.番茄耐热性研究进展.中国蔬菜,2003(2):58~60
    [2]王孝宣,李树德,东惠茹等.番茄品种耐寒性与ABA和可溶性糖含量的关系[J].园艺学报,1998,25(1):56~60
    [3]赵春江,康书江,王纪华等.植物内源激素与不同基因型小麦抗寒性关系的研究.华北农学报,2000,15(3):51~54
    [4]谢吉容,向邓云,梅虎等.南方红豆杉抗寒性的变化与内源激素的关系.西南师范大学学报(自然科学版),2002,27(2):231~234
    [5]罗正荣.植物激素与抗寒力的关系[J].植物生理学通讯,1989,3:1~5
    [6]陈玲,宋松泉,傅家瑞.番茄种子萌发的高温耐性诱导.种子,1998(5):10~12
    [7]Levy A, Rabinowitch H O. Kedar N. Morphological and Physiological characters affecting flower drop and fruit set of tomato at high temperature. Euphytica, 1978, 27:211~218
    [8]Atherton J.G., Rudich J. The tomato crop[M]. Chop-man and Hall, 1986, 75~77
    [9]Ahmadi A.B., Stevens M A. Reproductive responses of heat tolerant tomatos to high temperatures. J. Am. Soc. Horticultural Sci, 1979, 104(5): 686~691
    [10]A bdalla A A, Verkerk K. Growth, flowering and fruit-set of the tomato at high temperature. Neth. J. agric. Sci, 1968, 16:71~76
    [11]郑东虎,黄俊轩,王丽娟.番茄低温生态学的研究进展.北方园艺,2002(3):36~37
    [12]林多,魏毓棠,王世刚.番茄耐低温研究进展.沈阳农业大学学报,2000-12,31(6):585~589
    [13]杜永臣.弱光对番茄生育的影响[J].中国蔬菜,1996,(6):51~53.
    [14]王孝宣,李树德,东惠如等.低温胁迫对番茄苗期和花期若干性状的影响[J].园艺学报,1996,23(4):349~354
    [15]Dinar M, Rudich J, Zamski E. Effect of heat stress on carbon transport from tomato plants. Annals of Botany, 1983, 51: 97~103
    [16]Dinar M, Rudich J. Effect of heat stress on assimilates partitioning in tomato. Annals of Botany, 1985,56:239~248
    [17]Kuo C G, Chen H M, Ma L H. Effect of high Temperature on Proline Content in Tomato Floral Buds and Leaves. J. Amer.Soc. Hort.Sci. 1986, 111(5): 746~750
    [18]Douglas C M, Croxdate J, Markley J L. Thermal damage to chloroplast envelope membranes. Plant Physiology. 1989, 90:606~609
    [19]Weaver M L, Timm H. Screening tomato for high temperature tolerance through pollen viability tests. Hort.Sci. 1989, 24:493~495
    [20]Diae J, Cambell W F, seeley S D. Temperature-stress-induced production of abscisic acid and dihydrophasic acid in warm and cold season crops. J Amer Soc Hoet Sci, 1981,106:111~113
    
    
    [21] Sharma N K, Bhutani R D, Singh A, et al. Breeding tomato for heat tolerance—a review. Crop research. 1993, 6(1): 51~58
    [22] Li-F. Relationship between changes of endogenous ABA level and resistance in hybrid rice under temperature stress. Chinese-Rice-Research-Newsletter. 1994, 2: 1, 9
    [23] Bray E A.Wiid type levels of abscisic acid are required for heat shock protein accumulation in tomato. Plant Physiol, 1991, 97:817~820
    [24] 路子显.大豆热激蛋白与内源激素变化的研究.大豆科学.1998,17(4):318~325
    [25] Lu Zi-xian. Effect of Heat Stress on Indole-3-acetic Acid and Abscisic Acid Contents in Soybean of Various Developmental Stages. Developmental & Reproductive Biology. 1999,18 (1): 83~93
    [26] Iwahori S. High temperature injuries in tomato. Ⅳ. Development of normal flower buds and morphological abnormalities of flower buds treated with high temperature. J. Japan. Soc. Hort.Sci. 1965, 34:33~41
    [27] 王孝宣,李树德,东惠茹等.低温胁迫对番茄苗期和开花期脂肪酸的影响[J],园艺学报,1997,24(2):161~164
    [28] Chen H.H, Li D.H. Plant cold hardness and freezing stress. Academic press. 1982, 5
    [29]章文才.中国柑桔冻害研究.北京:农业出版社,1993,51~55
    [30] Baricevic D. Significance of abscisic acid (ABA) in low temperature stress in globe artichoke (Cynara scolymus L.) cultivars. Zbornik-Biotehniske-Fakultete-Univerze-v-Ljubljani, Kmetijstvo. 1995, 65:25-32
    [31] Wightman F. Plant regulation and world agriculture [M].N Y: Plenum Press, 1979. 327~371
    [32]林定波,刘祖祺.冷驯化与ABA对柑橘膜稳定性的影响及膜特异性蛋白质的诱导[J].南京农业大学学报.1994,17(1):1~5
    [33]蔡世英.脱落酸对咖啡幼苗抗冷性的效应[J].热带作物学报.1990,11(2):69~77
    [34]陈善娜,郭浙红,沈云光等.在低温胁迫下外源ABA对高原水稻自由基清除系统的影响[J].云南大学学报.1996,18(2):167~172
    [35]刘祖祺,张石城.植物抗性生理学[M].北京:中国农业出版社.1994:50~66
    [36]郭确,潘瑞枳.ABA对水稻幼苗抗冷性影响[J].植物生理学报,1984,10:295~304
    [37] Howell G S. Analysis and improvement of Plant Cold Hardness[M]. N Y: CRC Press, 1981, 175
    [38] Heber V, Kempfle M. Proteins als schutzstoffe gegenuber dem gefriertod der zelle[J].Naturforsch, 1975, 25b: 834~842
    [39]罗正荣.PP333对柑桔抗冻力以及叶片内源ABA和GA_3含量的影响.植物生理学通讯,1992,28(3):182~184
    [40]于贤昌,邢禹贤,马红等.低温胁迫下黄瓜嫁接苗和自根苗内源激素的变化.园艺学报,1999,26(6):406~407
    [41]刘进生,汪隆植,李式军.番茄耐热优良品种筛选初报.中国蔬菜,1994,6:33~35
    [42]尹贤贵,罗庆熙,王文强等.番茄耐热性鉴定方法研究.西南农业学报,2001,14(2):62~65
    
    
    [43]沈征言,朱海山.高温对菜豆生育及不同基因型耐热性的差异.中国农业科学,1993,26(3):50~55
    [44]Chen H Z, Shen Z Y. Adaptability of crop plants to high temperature stress. Crop Science, 1982,22:719~725
    [45]Martineau L. Temperature tolerance in soybean . 1. Evaluation of a technique for assessing cellular membrane thermostability. Crop Science, 1979, 19:75~78
    [46]司家钢.高温胁迫对大白菜耐热性相关生理指标的影响.中国蔬菜,1995(4):4~6;15
    [47]Manzano-Mendez J, Hicks, J R. Influence of store temperature and ethylene on firmness, acid, and sugars of chilling-sensitive and chilling-tolerant tomato. Journal of the American society for Horticultural Sci. 1984, 109(2): 273~277
    [48]Vernieri P, Tognoni F. Endogenous ABA, PA and DPA levels in chilled tomato seedlings. Acta Horticulture, 1988, 229:415~419
    [49]王孝宣,李树德,东惠茹等.低温对番茄苗期和开花期几种同工酶的影响[J].中国蔬菜,1996,(3):1~3
    [50]朱龙英,徐悌惟,康高强等.番茄耐低温和耐弱光性能鉴定方法初探.上海农业学报,1998,14(1):45~50
    [51]宋洪元,雷建军,李成琼.植物热胁迫反应及抗热性鉴定与评价.中国蔬菜,1998(1):48~50
    [52]李合生.植物生理生化实验原理和技术.北京:高等教育出版社,2000,261~263
    [53]李合生.植物生理生化实验原理和技术.北京:高等教育出版社,2000,258~260
    [54]李合生.植物生理生化实验原理和技术.北京:高等教育出版社,2000,194~197
    [55]Abdul Baki AA, Stommel-JR. Pollen viability and fruit set of tomato genotypes under optimumand high-temperature regimes. HortScience. 1995, 30: 1, 115~117
    [56]Malhotra SK, Kalloo G. Breeding tomato for low temperature fruit-set. Aricultural-Reviews-Kamal. 1995, 16: 1-2, 63~72
    [57]李合生.植物生理生化实验原理和技术.北京:高等教育出版社,2000,258
    [58]高吉寅,胡荣海.水稻等品种苗期抗旱生理指标的探讨.中国农业科学,1984,4:41~45
    [59]吴晓雷,尚春明,张学东等.番茄品种耐弱光性的综合评价.华北农学报,1997,12(2):97~101
    [60]王世刚.番茄耐低温性及其相关性状和遗传效应的分析:[硕士学位论文].沈阳:沈阳农业大学,2000
    [61]王孝宣,李树德,东惠如等.低温对番茄苗期和开花期几种同工酶的影响[J].中国蔬菜,1996,(3):1~3
    [62]朱龙英,徐悌惟,康高强等.番茄耐低温和耐弱光性能鉴定方法初探.上海农业学报,1998,14(1):45~50

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700