施钾水平对烤烟钾高效基因型新品系生长发育及产质量的影响
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摘要
为了探明施钾量对烤烟钾高效基因型新品系生长发育和产质量的影响,本试验以烤烟品种K326(对照),GK2、GK8(钾高效基因型新品系)为供试材料,于2009年在湖南农业大学烟草基地进行大田试验,分析比较了烟株的生育期、农艺性状、干物质积累量、生理生化特性、烤后烟叶的经济性状及内在化学成分在不同供钾水平下的差异。研究结果表明:
     1.施钾对烤烟钾高效基因型新品系生育期有明显影响。不施钾肥的烟株前期生长较慢,现蕾较迟;中后期烟叶缺钾症状明显,顶叶落黄较快,大田生育期明显缩短;不同品种(系)间GK2、GK8的大田生育期比K326略早。
     2.施钾可以明显改善烤烟钾高效基因型新品系生物学性状,促进烟株株高、茎围、叶片大小及单株叶数的增加。团棵期不同供钾水平下烟株的长相长势基本一致,进入旺长期后,施钾240 kg/hm2时,烟株长势最好,不同品种(系)间表现为:GK8>GK2>K326。
     3.烤烟钾高效基因型新品系根、茎、叶的干物质积累量随着施钾量的增加而增加。不同生育期烟株各器官干物质积累量表现为:叶>茎>根,不同供钾水平间表现为:T3>T2>T1;成熟期K326、GK2、GK8的T3处理与T1处理相比,烟叶干物质积累量分别增加了35.93%,36.45%,42.71%,不同品种(系)间表现为:GK8>GK2>K326。
     4.施钾促进了烤烟钾高效基因型新品系生长前期合成叶绿素的能力,提高了烤烟钾高效基因型新品系硝酸还原酶的活性,并对过氧化物酶活性有一定的调节作用。
     5.施钾对烤烟钾高效基因型新品系的经济性状影响明显,增施钾肥能够提高烟叶的产量、产值和中上等烟的比例。施钾240 kg/hm2时产量最高,K326、GK2、GK8分别达到2353.02 kg/hm2,2422.57 kg/hm2,2478.09 kg/hm2,烤烟产值分别比不施钾处理高出69.92%,64.30%,69.97%,中上等烟比例K326(88.14%)表现最好。
     6.烤后烟叶化学成分测定结果表明,烤烟钾高效基因型新品系烟叶总糖、还原糖、钾含量随着供钾水平的提高而增加,烟叶总氮、烟碱、氯离子含量则减少;在施钾240 kg/hm2时,K326、GK2、GK8烟叶钾含量分别达到2.05%,2.46%,2.37%,糖碱比、钾氯比、糖氮比适宜,烟叶品质最好。
In order to investigate the effects of potassium application rate on the growth and yield and quality of new strains of flue-cured tobacco genotypes with high potassium efficiency. Field experiments with flue-cured tobacco K326(CK)and GK2,GK8(flue-cured tobacco genotypes with high potassium efficiency)were conducted in tobacco cultivation base of Hunan Agricultural University in 2009.The experiments analyzed the difference of growth period, agronomical traits, dry matter accumulation of tobacco, physiological and biochemical characteristics, economic characteristics of tobacco and chemical composition of cured leaves in different potassium application rate. The results showed that:
     1. The growth period of new strains of flue-cured tobacco genotypes with high potassium efficiency was obviously affected by potassium application. The tobacco without potassium fertilizer grew slowly and the bud appeared later; The symptoms of potassium deficiency was obvious,the leaves yellowing faster in late and the field growth period was Significantly shortened; The field growth period of the GK2 and GK8 slightly earlier than the K326.
     2. Potassium application could significantly improve the biological properties of the new strains, promote the increase of plant height, stem thickness, leaf size and leaf number. Growing tobacco looked basically the same under different potassium levels in the rosette stage.The tobacco plants grew best under the potassium level of 240 kg/hm2 in the vigorous growing period, in different genotypes showed:GK8>GK2>K326。
     3. The dry matter accumulation of the new strains roots, stems and leaves increased with potassium application rate increased. Amount of dry matter accumulation in different organs showed:leaf>stem>root, between the different potassium levels showed: T3>T2>T1. T3compared with T1, the dry matter accumulation of leaves of K326,GK2 and GK8 respectively increased by 35.93%,36.45%,42.71% in the mature stage. In different genotypes showed:GK8>GK2>K326。
     4. Potassium application could promote the ability of chlorophyll synthesis, increase the activity of NR, adjust the activity of POD to some extent.
     5. Economic characteristics of the new strains significantly affected by potassium application. Potassium fertilizer could increase the yield, output and proportion of upper-middle of the new strains. The new strains in the highest yield under the potassium level of 240 kg/hm2 and K326,GK2,GK8 respectively reached 2353.02 kg/hm2,2422.57 kg/hm2,2478.09 kg/hm2. The tobacco production of K326, GK2 and GK8 under the potassium level of 240 kg/hm2 were higher than the treatment without potassium fertilizer to 69.92%,64.30%,69.97%. The proportion of mid-upper leaves of K326 achieved the best performance of 88.14%.
     6. Determination of chemical composition of cured leaves showed that the content of total sugar, reducing sugar, potassium of the new strains increased with potassium application rate increased, the content of nitrogen, nicotine, chloride of the new strains decreased with potassium application rate increased; The potassium content of K326,GK2 and GK8 respectively reached 2.05%,2.46%,2.37%;The ratio of reducing sugar and nicotine, potassium and chloride, total sugar and nitrogen were all proper; The quality of tobacco is the best under the potassium level of 240 kg/hm2.
引文
[1]胡国松,赵元宽,曹志洪,等.我国主要烟草烤烟元素组成和化学品质评价[J].中国烟草学报,1997,3(3):36-43.
    [2]朱尊权.当前我国优质烤烟生产中存在的问题[J].烟草科技,1999,(2):1-7.
    [3]朱尊权.烟叶的可用性与卷烟的安全性[J].烟草科技,2000,(8):326-327.
    [4]叶协锋,杨超,刘国顺,等.烟草钾素研究进展[J].河南农业科学,2004,(11):15-19.
    [5]曹志洪等.我国烟叶含钾状况及其与植物土壤环境条件的关系[J].中国烟草,1990,(1):1-6.
    [6]陈建军.提高烟叶钾含量技术途径的探讨[J].中国烟草科学,1999,(4):1-4.
    [7]赵文明,戴建军,丁伟,等.不同施钾水平对烤烟产质量影响的研究[J].东北农业大学学报,1999,30(1):41-43.
    [8]刘华山,王方,韩锦峰,等.烟草钾素营养的研究进展[J].安徽农学,2006,34(19),4961-4962.
    [9]曹志洪,周秀如,李仲林,等.我国烟叶含钾状况及其植烟土壤环境条件的关系[J].中国烟草,1990,(3):6-13.
    [10]李天福,陈萍,冉邦定.烟叶含钾量与土理性状的关系[P].烟草科学研究,2000,(3):17-19
    [11]颜丽,关连珠,奕双,等.土壤供钾状况及土壤湿度对我国北方烤烟烟叶含钾量的影响研究[J].土壤学通报,2001,(2):84-87.
    [12]李智勇,刘思英,丁一,等.土壤速效钾含量与烤烟钾施用效果的研究[J].中国烟草,1996,(1):40-44.
    [13]赵立红.烟草种质资源烟叶化学成分的初步分析[J].中国种业,2006,(5):37.
    [14]李廷轩,马国瑞.籽粒苋富钾品种(系)筛选研究[J].植物营养与肥料学报.2003,9(4):473-479.
    [15]苏贤坤,张晓海,汪自强.烤烟钾素营养特性的品种(系)差异研究[J].植物营养与肥料学报,2005,(4):536-540.
    [16]齐永杰,刘光辉,李群岭,等.不同品种(系)烤烟体内钾积累分配差异比较研究[J].广西农业科学,2008,(5):632-635.
    [17]胡国松等.烤烟营养原理[M].科学出版社,2000.
    [18]钱晓刚,杨俊,朱瑞.烟草营养与施肥[M].贵州科技出版社,1998.
    [19]吴平、印莉萍等.植物营养分子生理学[M].科学出版社,2001.
    [20]浙江农业大学主编.植物营养与施肥[M].农业出版社,1991.
    [21]汪邓民,周冀衡,朱显灵.干旱胁迫下钾对烤烟生长及抗旱性的生理调节[J].中国烟草科学,1998,19(3):26-29.
    [22]周翼衡,李卫芳,王月丹,等.钾对病毒侵染后叶片内源保护酶活性的影响[J].中国农业科学,2000,(6):98-100.
    [23]叶协锋,刘国顺等.不同钾肥施用量对烤烟生长过程中几种酶活性的影响[J].华北农学报,2004,19(3):88-91.
    [24]牛佩兰,石屹.烟草品种(系)间钾效率差异研究初报[J].烟草科技,1996(1):33-35.
    [25]周应兵,林国平.钾积累高效烟草种质资源的鉴定[J].安徽农业技术师范学院学报,1998,12(3):21-25.
    [26]杨铁钊,范进华.不同品种(系)烤烟品种吸收钾差异的根系特性研究[J].西北农业学报,2006,5(3):41-44.
    [27]王永锐,陈玉梅,邓政寰.耐低钾水稻幼苗生长及其营养吸收状况[J].中山大学学报:自然科学版,1989,(4):68-73.
    [28]施卫明,王校常,严蔚东,等.外源钾通道基因在水稻中的表达及其钾吸收特征研究[J].作物学报,2002,(3):374-378.
    [29]邹春琴,李振声,李继云.小麦对钾高效吸收的根系形态学和生理学特征[J].植物营养与肥料学报,2001,7(1):36-43.
    [30]王巩,陆引罡,张步阔,等.不同水稻品种对钾的吸收生理特性研究[J].耕作与栽培,2001,(2):25-26.
    [31]杨铁钊,彭玉富.富钾品种(系)烤烟钾积累特征研究植物[J].营养与肥料学报,2006,12(5):750-753.
    [32]曹享云.营养胁迫与根系分泌物[J].土壤学进展,1994,22(3):27-33.
    [33]王巩,陆引罡,张步阔,等.不同水稻品种对钾的吸收生理特性研究[J].耕作与栽培,2001,(2):25-26.
    [34]Mengel K. Response of various crop species and cultivars fertilizer application[J]. Plant Soil,1983,(72):305-319.
    [35]杨铁钊.烟草育种学[M].北京:中国农业出版社,2003.
    f36]陈明灿,李友军.不同施钾量对烟草生育及产、质的影响.洛阳农业高等专科学校学报[J].2002,22(4):241-242.
    [37]张燕成,罗以贵,吴永明.不同施钾量对烤烟产质量及焦油含量的影响[J].安徽农业科学,2009,37(7):3065-3067.
    [38]戴勋,王毅,刘彦中,等.不同钾肥追施量对烤烟K326生长及产质量的影响[J].中国烟草科学,2009,30(1):19-22.
    [39]罗建新,肖汉乾,彭建伟,等.施钾方法对土壤供钾能力及烤烟钾累积的影响[J].湖南农业大学学报,2000,26(5):352-354.
    [40]程辉斗,温永琴,陆富,等.土壤供钾水平与云南烤烟含钾量关系的研究[[J]烟草科技,2000,(3):41-43.
    [41]张翔,黄元炯,范艺宽,等.河南省植烟土壤与烤烟施肥的现状、存在问题及对策[J].河南农业科学,2004,00:54-57.
    [42]王同超,刘作新,高致明,等.分期追施钾肥对烤烟生长和品质的影响[J].河南农业大学学报,2002,36(4):348-351.
    [43]赵久明,戴建军.不同施钾水平对烤烟产质量影响的研究[J].东北农业产学学报,1999,30(10):41-43.
    [44]韩锦峰,杨素勤,干永华等.不同相伴阴离子钾肥对烤烟光合特性、钾含量及化学成分的影[J].中国烟草学报,2002,8(3):22-25.
    [45]方智勇,朱晓兰.烤烟中N,P,K三要素的含量及其有关因素的分析[J].安徽农业科学,1999,27(4):357-359.
    [46]杨铁钊,晃逢春,丁永乐等.烟草不同品种(系)叶片钾积累特性及变异分析.中国烟草学报,2002,8(3):11-16.
    [47]唐永红.烤烟产质量与施钾量及留叶数变化规律的研[J].陕西农业科学,1998(3):23-24.
    [48]慕成功.钾素营养及施肥技术[M].中国农业科技出版社,1995.
    [49]左天觉著,朱尊权译.烟草的生产、生理和生物化学[M].上海:上海远东出版社,1993:30-66.
    [50]REN TB,ZHAOJ X. Effect of different nitrogen application amount on the net increment of fresh dry weight in hybrid rape[J].Agricultural Science & Tech2 nology,2008,9(2):147-151.
    [51]胡荣海,邵岩.云南烟草栽培学[M].北京:科学出版社,2007.
    [52]曹志洪,胡国松等土壤供钾特性和烤烟的钾肥有效使用[J].烟草科技,1993,(2):33-37
    [53]Akehurst B. C. Tobacco, Humanities Press, NewYork,1981,387-398.
    [54][美]W. K. Collins, S. N. Hawks著,陈江华,杨国安译.烤烟生产原理[M].科学技术文献出版社,1995.
    [55]汪波,屠兢.钾盐在烟草燃烧过程中的作用[J].中国科学技术大学学报,2002,132(4)57-59.
    [56]胡笃敬,杨敏元,刘国华.高钾植物研究[J].湖南农业大学学报(自然科学版),1980,4:5-13.
    [57]佟道儒.烟草育种学[M].北京:中国农业出版社,1997.
    [58]Andreson J A, Huprikar S S, Kochian L V, et al. Functional expression of a probable A rabi dopsis thaliana potassium channel in S. Cerevisiae. Proc Natl Acad Sci USA,1992,89:3736-3740.
    [59]施卫明,严蔚东,黄骏麒,等.钾高效利用型转基因棉花的培育[J].江苏农业学报,2001,17(3):188-189.
    [60]Shi W M, Su Y H, Fujiwara T, et al. Transfer of potassium channel genes into tobacco. In:Andoetal T ed. Plant nutrition for sustainable food production and environment. Tokyo:Kluwer Academic Publishers,1997,195-196.
    [61]Bei Q, Luan S. Functional expression and characterization of a plant K+ channel gene in a plant cell model. Plant,1998,13:857-865.
    [62]Ichida A M. Expression of a Ca+-resistant guard cell K+ channel confer Ca+ resistant, light-induced stomatal opening in transgenic A rabi dopsis [J]. Plant Cell, 997,9(10):1843-1857.
    [63]郭兆奎,杨谦,姚泉洪.HAL1基因转化烟草提高烟叶含钾量研究[J].中国烟草学报,2006,10(5):46-51.
    [64]HESS. D. Pollen based techniques in genetics manipulation[J]. Int.Rev.Cytol,1987, 107:376-395.
    [65]周光宇,龚蓁蓁.远缘杂交的分子基础-DNA片段杂交假设的一个论证[J].遗传学报,1979,6(4):405-413.
    [66]黄骏麒,钱思颖,周光宇,等.外源抗枯萎病棉DNA导入感病棉的抗性转移[J].中国农业科学,1986,3:32-36.
    [67]雷勃军,钱毕,李希臣.通过直接引入外源DNA育成高产,优质,高蛋白大豆新品种:黑生101[J].作物学报,2000,26(6):725-730.
    [68]杨铁钊.烟草育种学[M].北京:中国农业出版社,2003.
    [69]张立军,樊金娟.植物生理学实验教程[M].北京:中国农业出版社,2007.
    [70]郝再彬.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004.
    [71]王瑞新.烟草化学[M].北京:中国农业出版社,2006.
    [72]王瑞新,韩富根,杨素勤.烟草化学品质分析法[M].郑州:河南科学技术出版社,1990.
    [73]鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000.
    [74]钟晓兰.施钾对烤烟钾素吸收利用效率及其产量和品质的影响[J].2008,40(2):216-221.
    [75]颜合洪,胡雪平,张锦韬,等.不同施钾水平对烤烟生长和品质的影响[J].2005,31(1):20-24.

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