中年女性体力活动与端粒体的相关性及“万步行”干预效果的研究
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摘要
目的分析40-59岁中年女性体力活动与端粒体长度的关系；通过“万步行”活动,研究基础PA水平不同的中年女性健身效果。方法实验1：71名40-59岁女性佩戴7天的加速度计,计算不同强度PA时间,测空腹血糖、血脂四项、外周血白细胞端粒体长度(T/S),研究PA与T/S值相关性。实验2：筛选出30名受试者,按照加速度计的结果分PA活跃和不足组,实施12周“万步行”活动。结束后分析受试者日行万步的完成情况、每日平均增加的步数,研究PA增加对机体身体成分、糖脂代谢、心肺耐力等的影响及存在的差异。结果实验1：(1)40-59岁女性52.11%的人属于PA不足,其中40-49岁达67.86%；(2)超重肥胖中72.73%存在PA不足,正常体重只有34.21%；(3)PA不足的血糖、TC、TG均高于PA活跃组,P<0.05, LDL-C明显高于活跃组P<0.01, HDL-C较PA活跃组低P<0.05；(4)年龄与T/S值的相关系数是-0.364,40-49岁、50-59岁PA水平与T/S值的R2分别为0.224、0.0719,BMI与T/S值相关系数是0.17,P值为0.156。实验2：(1)PA活跃和不足组“万步行”达标率分别为88.24%和46.15%,P<0.05；(2)PA不足组每日增加的步数多于活跃组(5427±2174VS3087±1281)；(3)干预后,两组身体成分、血糖、TC、TG、LDL-C和HDL-C均有显著性改善,PA不足组改善程度最显著(P<0.05)；(4)干预后PA不足和活跃组F.C分别提高1.07MET和0.63MET, P<0.01;(5)干预后,PA不足组的左、右PWV分别减慢92cm/s、63cm/s, PA活跃组PWV无变化。(6)在相同负荷下,两组的心率、SBP均较干预前下降,PA不足组的下降幅度最为明显,P<0.01。结论(1)中年女性50%存在PA不足,每周进行VPA的时间几乎为Omin；(2)40-49岁、超重肥胖女性的PA水平较低：(3)年龄、PA水平对端粒体长度影响较大,BMI的影响较小；(4)F.C水平较高者端粒体长度较长；(6)“万步行”活动对PA不足的女性改善效果更显著；(7)对基础PA水平较低的女性,只要能增加日行总步数,即使没有达到一万步的要求,也能获得较多的益处。
Purpose:The relationship between the level of physical activity (PA) and telomere length of40-59year-old women was analyzed, and the role of PA played on the body's anti-aging was explored; By the implementation of12weeks'ten-thousand footsteps activity, the fitness effect of different PA levels on middle-aged women was discussed.
Method:Experiment1:Seventy-one40-59year-old women were required to wear accelerometer for seven days. Then the PA time of different intensity each day was calculated, fasting blood-glucose and four blood lipids indicators (TC, TG, HDL-C, and LDL-C) were tested, relative length of telomeres of peripheral blood leukocyte by the method of real-time fluorescent quantitation PCR was measured to analyze factors influencing T/S values and the relativity between PA and T/S value. The research was approved by the regional Ethics Committee.Written informed consent was obtained from each participant.
Experiment2:Thirty participants were picked out from all of the experiment1participants, and divided into PA active group and PA insufficient group according to the accelerometer testing results, and then implement12weeks'ten-thousand footsteps activity to both groups and recorded the total step number each day by pedometer. After the end of the activity, the completion state of ten-thousand footsteps everyday task for both groups were analyzed based on the record sheet, and the average increasing steps for one day was calculated to study the effects of this intervention on body composition, glucose and lipid metabolism, cardiorespiratory endurance and differences between the two groups.
Results:Experiment1:(1) Among these40-59year-old women,52.11%were lack of PA, and67.86%among40-49year-old women;(2)72.73%of the overweight women were lack of PA, while only34.21of this for normal weight women;(3) The serum glucose, TC, TG of PA insufficient group participants were higher than that of PA active group with a significant differences (P<0.05), and LDL-C also significantly higher than that of PA active group (P<0.01).As to HDL-C, it is lower of PA insufficient group than active group (P<0.05);(4) The relationship between age and T/S value shows a negative correlation with-0.364as correlation coefficient, and P=0.002. As for40-49year-old women, PA level was positively correlated with T/S value, R2=0.224, and for50-59year-old women, there showed a low correlation between PA level and T/S value, R2=0.0719, the correlation coefficient of BMI and T/S value was0.17, P=0.156. Experiment2:(1) The completion rate of PA active group for ten thousand footsteps walking task was88.24%, and46.15%for the PA insufficient group, which showed a significant difference (P<0.05) between the two groups;(2) The average increasing number in one day for PA insufficient group was significantly more than that of PA active group (5427±2174VS3087±1281);(3) After the exercise intervention, the body composition, blood glucose, TC, TG, LDL-C and HDL-C of participants in both groups were improved significantly, with a more significant improvement for PA insufficient group(P<0.05);(4) After the exercise intervention, the cardiorespiratory endurance for PA insufficient group rose from7.70MET to8.77MET, and8.19MET to8.82MET for PA active group,
respectively increased1.07MET and0.63MET with a significant difference(P<0.01);(5) After the exercise intervention, ABI for both group showed no significant change (P>0.05). The left and right PWV slowing down degree for PA insufficient group was respectively92cm/s (P<0.05), and63cm/s, while there showed no significant change for that of PA active group.(6) Under the same exercise load, heart rate, systolic pressure increasing rate for both groups were declined, with a more significant decline for PA insufficient group and showed significant difference (P<0.01).
Conclusions:(1)50%middle-aged women have PA insufficient problem, and the time for VPA each week is almost Omin;(2) PA level for40-49year-old women are significantly lower than that of50-59year-old women, and overweight women lower than normal weight women;(3) Age and PA level would affect telomere length of human peripheral blood leukocyte, but the effect of BMI on telomere length was quite small;(4) The telomere length of PA active person was significantly longer than that of PA insufficient person;(5)The telomere length of high cardiorespiratory endurance person was significantly longer than that of low cardiovascular endurance person;(6) The ten thousand footsteps walking can benefit the body composition and adjust the glucose and lipid metabolism of middle-aged women, especially for those lack of PA;(7) The ten thousand footsteps walking can significantly improve the cardiovascular endurance of middle-aged women, and shows a regulating effect on quantitative cardiovascular exercise reaction;(8) For those lack of basic PA middle-aged women, as long as largely increases the total number of steps for a day, even not complete the ten thousand footsteps task, their health could also benefit a lot. Compared to the absolute value of daily total steps number, daily footstep increasing number has a greater effect on health.
Purpose:The relationship between the level of physical activity (PA) and telomere length of40-59year-old women was analyzed, and the role of PA played on the body's anti-aging was explored; By the implementation of12weeks'ten-thousand footsteps activity, the fitness effect of different PA levels on middle-aged women was discussed.
Method:Experiment1:Seventy-one40-59year-old women were required to wear accelerometer for seven days. Then the PA time of different intensity each day was calculated, fasting blood-glucose and four blood lipids indicators (TC, TG, HDL-C, and LDL-C) were tested, relative length of telomeres of peripheral blood leukocyte by the method of real-time fluorescent quantitation PCR was measured to analyze factors influencing T/S values and the relativity between PA and T/S value. The research was approved by the regional Ethics Committee.Written informed consent was obtained from each participant.
Experiment2:Thirty participants were picked out from all of the experiment1participants, and divided into PA active group and PA insufficient group according to the accelerometer testing results, and then implement12weeks'ten-thousand footsteps activity to both groups and recorded the total step number each day by pedometer. After the end of the activity, the completion state of ten-thousand footsteps everyday task for both groups were analyzed based on the record sheet, and the average increasing steps for one day was calculated to study the effects of this intervention on body composition, glucose and lipid metabolism, cardiorespiratory endurance and differences between the two groups.
Results:Experiment1:(1) Among these40-59year-old women,52.11%were lack of PA, and67.86%among40-49year-old women;(2)72.73%of the overweight women were lack of PA, while only34.21of this for normal weight women;(3) The serum glucose, TC, TG of PA insufficient group participants were higher than that of PA active group with a significant differences (P<0.05), and LDL-C also significantly higher than that of PA active group (P<0.01).As to HDL-C, it is lower of PA insufficient group than active group (P<0.05);(4) The relationship between age and T/S value shows a negative correlation with-0.364as correlation coefficient, and P=0.002. As for40-49year-old women, PA level was positively correlated with T/S value, R2=0.224, and for50-59year-old women, there showed a low correlation between PA level and T/S value, R2=0.0719, the correlation coefficient of BMI and T/S value was0.17, P=0.156. Experiment2:(1) The completion rate of PA active group for ten thousand footsteps walking task was88.24%, and46.15%for the PA insufficient group, which showed a significant difference (P<0.05) between the two groups;(2) The average increasing number in one day for PA insufficient group was significantly more than that of PA active group (5427±2174VS3087±1281);(3) After the exercise intervention, the body composition, blood glucose, TC, TG, LDL-C and HDL-C of participants in both groups were improved significantly, with a more significant improvement for PA insufficient group(P<0.05);(4) After the exercise intervention, the cardiorespiratory endurance for PA insufficient group rose from7.70MET to8.77MET, and8.19MET to8.82MET for PA active group,
respectively increased1.07MET and0.63MET with a significant difference(P<0.01);(5) After the exercise intervention, ABI for both group showed no significant change (P>0.05). The left and right PWV slowing down degree for PA insufficient group was respectively92cm/s (P<0.05), and63cm/s, while there showed no significant change for that of PA active group.(6) Under the same exercise load, heart rate, systolic pressure increasing rate for both groups were declined, with a more significant decline for PA insufficient group and showed significant difference (P<0.01).
Conclusions:(1)50%middle-aged women have PA insufficient problem, and the time for VPA each week is almost Omin;(2) PA level for40-49year-old women are significantly lower than that of50-59year-old women, and overweight women lower than normal weight women;(3) Age and PA level would affect telomere length of human peripheral blood leukocyte, but the effect of BMI on telomere length was quite small;(4) The telomere length of PA active person was significantly longer than that of PA insufficient person;(5)The telomere length of high cardiorespiratory endurance person was significantly longer than that of low cardiovascular endurance person;(6) The ten thousand footsteps walking can benefit the body composition and adjust the glucose and lipid metabolism of middle-aged women, especially for those lack of PA;(7) The ten thousand footsteps walking can significantly improve the cardiovascular endurance of middle-aged women, and shows a regulating effect on quantitative cardiovascular exercise reaction;(8) For those lack of basic PA middle-aged women, as long as largely increases the total number of steps for a day, even not complete the ten thousand footsteps task, their health could also benefit a lot. Compared to the absolute value of daily total steps number, daily footstep increasing number has a greater effect on health.
引文
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