针刺“百会”透“曲鬓”对脑出血模型大鼠脑组织细胞凋亡的作用及机制研究
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摘要
目的：探讨针刺“百会”透“曲鬓”对脑出血模型大鼠脑组织细胞凋亡的作用机制。
方法：将Wistar大鼠随机分为正常组、模型组6h、模型组1d、模型组3d、模型组7d、针刺组6h、针刺组ld、针刺组3d、针刺组7d,共9组,每组6只大鼠。其中模型组和针刺组采用脑内自体血注入法制备脑出血模型,针刺组为造模后给予针刺“百会”透“曲鬓”治疗。对各组大鼠进行实验观察：
(1)观察各组实验大鼠神经功能缺损评分情况；
(2)光镜下观察各组大鼠HE染色脑组织病理形态改变；TUNEL法检测各组大鼠脑出血半暗带区细胞凋亡情况；
(3)应用免疫组织化学、免疫蛋白印迹方法分别检测各组大鼠脑组织中p38MAPK及p-p38MAPK在脑出血半暗带区的动态表达；
(4)应用免疫组织化学方法检测各组大鼠脑组织中Bcl-2.Caspase-3在脑出血半暗带区的动态表达。
结果：
(1)针刺组可见神经功能缺损评分明显改善,在时间点3d、7d时与模型组比较差异有统计学意义(P<0.05)；
(2)HE染色结果：与模型组比较,针刺组神经细胞肿胀和间质水肿减轻,神经元数量增多。TUNEL法检测结果：与模型组比较,针刺组各时间点细胞凋亡数量均减少,且在3d、7d时间点差异有统计学意义(P<0.05)；
(3)免疫组织化学、免疫蛋白印迹结果：与模型组比较,针刺组脑组织各时间点p38MAPK及p-p38MAPK蛋白表达水平明显降低(P<0.05)；
(4)免疫组织化学结果显示：与模型组比较,针刺组脑组织各时间点Bc1-2蛋白表达水平升高、Caspase-3蛋白表达水平降低(P<0.05)。
结论：
(1)“百会”透“曲鬓”可以减轻脑出血模型大鼠神经功能缺损症状；
(2)“百会”透“曲鬓”对大鼠脑出血后继发性脑损伤的神经细胞凋亡有抑制作用；
(3)“百会”透“曲鬓”能够抑制与细胞凋亡密切相关的p38MAPK信号转导通路的活化,对脑出血半暗带区脑组织有保护作用；
(4)“百会”透“曲鬓”针刺方法抗脑出血细胞凋亡的机制与促进半暗带Bcl-2蛋白表达,抑制Caspase一3蛋白表达相关。
Objective:To observe the mechanism of scalp point-through-point acupuncture on apoptosis in brain tissue of model rats with cerebral hemorrhage.
Method:Wistar rats were randomly divided into normal group, model group and acupuncture group, each group according to different time after making model and randomly divided into6h、1d、3d、7d four time points. The rats having been injected autologous blood to creat cerebral hemorrhage model,acupuncture group were given "Baihui Qubin acupoint" treatment. Observe in rats on each time point.(1) To observe the score of neural function defect at different time points in each group of rats;(2) To observe the HE rats brain tissue pathological changes of dyeing under the light microscope; Penumbra apoptosis was detected by TUNEL assay;(3)Immunohistochemistry and Western blot method are used to detect expression of p38MAPK and P-p38MAPK in brain tissue of rats in each group dynamics in the penumbra of cerebral hemorrhage;(4)Expression in brain tissue of rats were detected by immunohistochemical method in Caspase-3, Bcl-2dynamic in the penumbra of cerebral hemorrhage.
Results:(1)Score of neural function defect in acupuncture group showed obvious improvement,in phase3d,7d when compared with the model group, the difference was statistically significant (p<0.05);(2)The results of HE staining:compared with the model group, acupuncture group of nerve cell swelling,interstitial edema, neuron number increase.TUNEL test results: compared with the model group, acupuncture group at each time point the number of apoptotic cells were reduced, and there was significant in3d,7d phase difference (p<0.05);(3)Immunohistochemistry,Western blot results: compared with the model group,the brain tissue of acupuncture group at each time point of p38MAPK and p-p38MAPK protein expression levels were significantly lower,the difference was statistically significant(p<0.05);(4)Immunohistochemistry showed:compared with the model group,the brain tissue of acupuncture group at each time point Caspase-3protein expression decreased, Bcl-2protein expression level increased, and there was significant difference in some time points (p<0.05).
Conclusion:(1)The scalp point-through-point acupuncture method can reduce the symptoms of nerve function defect of cerebral hemorrhage rats;(2) The scalp point-through-point acupuncture has inhibitory effect on nerve cell apoptosis of secondary brain injury after intracerebral hemorrhage in rats;(3) Scalp point-through-point acupuncture can inhibit the activation of P38MAPK signal transduction pathway which is closely related to cell apoptosis;(4)The scalp point-through-point acupuncture can inhibit the expression of penumbra region of Caspase-3protein of cerebral hemorrhage, promote the expression of Bcl-2protein, to play the role of anti apoptosis.
方法：将Wistar大鼠随机分为正常组、模型组6h、模型组1d、模型组3d、模型组7d、针刺组6h、针刺组ld、针刺组3d、针刺组7d,共9组,每组6只大鼠。其中模型组和针刺组采用脑内自体血注入法制备脑出血模型,针刺组为造模后给予针刺“百会”透“曲鬓”治疗。对各组大鼠进行实验观察：
(1)观察各组实验大鼠神经功能缺损评分情况；
(2)光镜下观察各组大鼠HE染色脑组织病理形态改变；TUNEL法检测各组大鼠脑出血半暗带区细胞凋亡情况；
(3)应用免疫组织化学、免疫蛋白印迹方法分别检测各组大鼠脑组织中p38MAPK及p-p38MAPK在脑出血半暗带区的动态表达；
(4)应用免疫组织化学方法检测各组大鼠脑组织中Bcl-2.Caspase-3在脑出血半暗带区的动态表达。
结果：
(1)针刺组可见神经功能缺损评分明显改善,在时间点3d、7d时与模型组比较差异有统计学意义(P<0.05)；
(2)HE染色结果：与模型组比较,针刺组神经细胞肿胀和间质水肿减轻,神经元数量增多。TUNEL法检测结果：与模型组比较,针刺组各时间点细胞凋亡数量均减少,且在3d、7d时间点差异有统计学意义(P<0.05)；
(3)免疫组织化学、免疫蛋白印迹结果：与模型组比较,针刺组脑组织各时间点p38MAPK及p-p38MAPK蛋白表达水平明显降低(P<0.05)；
(4)免疫组织化学结果显示：与模型组比较,针刺组脑组织各时间点Bc1-2蛋白表达水平升高、Caspase-3蛋白表达水平降低(P<0.05)。
结论：
(1)“百会”透“曲鬓”可以减轻脑出血模型大鼠神经功能缺损症状；
(2)“百会”透“曲鬓”对大鼠脑出血后继发性脑损伤的神经细胞凋亡有抑制作用；
(3)“百会”透“曲鬓”能够抑制与细胞凋亡密切相关的p38MAPK信号转导通路的活化,对脑出血半暗带区脑组织有保护作用；
(4)“百会”透“曲鬓”针刺方法抗脑出血细胞凋亡的机制与促进半暗带Bcl-2蛋白表达,抑制Caspase一3蛋白表达相关。
Objective:To observe the mechanism of scalp point-through-point acupuncture on apoptosis in brain tissue of model rats with cerebral hemorrhage.
Method:Wistar rats were randomly divided into normal group, model group and acupuncture group, each group according to different time after making model and randomly divided into6h、1d、3d、7d four time points. The rats having been injected autologous blood to creat cerebral hemorrhage model,acupuncture group were given "Baihui Qubin acupoint" treatment. Observe in rats on each time point.(1) To observe the score of neural function defect at different time points in each group of rats;(2) To observe the HE rats brain tissue pathological changes of dyeing under the light microscope; Penumbra apoptosis was detected by TUNEL assay;(3)Immunohistochemistry and Western blot method are used to detect expression of p38MAPK and P-p38MAPK in brain tissue of rats in each group dynamics in the penumbra of cerebral hemorrhage;(4)Expression in brain tissue of rats were detected by immunohistochemical method in Caspase-3, Bcl-2dynamic in the penumbra of cerebral hemorrhage.
Results:(1)Score of neural function defect in acupuncture group showed obvious improvement,in phase3d,7d when compared with the model group, the difference was statistically significant (p<0.05);(2)The results of HE staining:compared with the model group, acupuncture group of nerve cell swelling,interstitial edema, neuron number increase.TUNEL test results: compared with the model group, acupuncture group at each time point the number of apoptotic cells were reduced, and there was significant in3d,7d phase difference (p<0.05);(3)Immunohistochemistry,Western blot results: compared with the model group,the brain tissue of acupuncture group at each time point of p38MAPK and p-p38MAPK protein expression levels were significantly lower,the difference was statistically significant(p<0.05);(4)Immunohistochemistry showed:compared with the model group,the brain tissue of acupuncture group at each time point Caspase-3protein expression decreased, Bcl-2protein expression level increased, and there was significant difference in some time points (p<0.05).
Conclusion:(1)The scalp point-through-point acupuncture method can reduce the symptoms of nerve function defect of cerebral hemorrhage rats;(2) The scalp point-through-point acupuncture has inhibitory effect on nerve cell apoptosis of secondary brain injury after intracerebral hemorrhage in rats;(3) Scalp point-through-point acupuncture can inhibit the activation of P38MAPK signal transduction pathway which is closely related to cell apoptosis;(4)The scalp point-through-point acupuncture can inhibit the expression of penumbra region of Caspase-3protein of cerebral hemorrhage, promote the expression of Bcl-2protein, to play the role of anti apoptosis.
引文
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