永久性栓塞材料的实验研究
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摘要
血管栓塞治疗解决了临床许多棘手的难题,选择恰当的栓塞材料对可靠的疗效十分重要。目前栓塞材料大部分依靠进口,昂贵的价格限制了临床应用,迫切需要国产化研究。研究合作实验室掌握了PVA制备的关键技术与环节,并能根据需要制备出各种大小的PVA颗粒。对材料的亲水性、吸水倍数、吸水后的膨胀变形率和密度等重要物理性能均进行了测试,得到了科学的数据。在自制PVA颗粒与进口PVA颗粒电镜比较时,两者形态结构相似。
体外毒性实验研究证实了自制PVA颗粒的安全性。以进口PVA颗粒作为对照组,采用介入导管栓塞技术行兔右肾动脉栓塞来考察栓塞效果。在影像学上应用CTA和DSA进行评价,在组织病理学上观察栓塞材料对栓塞动脉及肾实质的影响。研究结果表明:自制PVA颗粒具有较好的组织相容性,与进口PVA颗粒有相似的栓塞效果。所以自制PVA颗粒可作为血管栓塞剂,但在临床应用上还需进一步深入研究。
本实验研究首次应用CTA检查评价栓塞效果,证实了其与DSA具有相似的效果,为栓塞材料的研发找到了合适的、准确的评价方法;与此同时,也完善了导管栓塞技术,明确颈动脉插管是对股动脉插管失败后的一个有效的弥补方法。
Objective
To study the preparation methods and physical-chemical properties of PVA material; To assess the safety and embolic effect of self-made PVA particles as arterial embolic agent. To compare the efficacies of different imaging methods in evaluating embolic effect and summarize the experimental experiences of transcatheter renal arterial embolization in rabbits.
Materials and Methods
1 Laboratory preparation of self-made PVA material
We developed a method to prepare PVA absorbent material by mixing Polyvinylalcohol together with formaldehyde with rapid agitation. The surface density, multiplication after absorbing water, volume increase rate after absorbing water, climbing height after absorbing water and its morphological structure were measured.
2. Animal experiment of prepared PVA particles
2.1 Sterile test, fever-induced test, blood compatiblity, histocompatiblity test and toxic test were done for prepared PVA particles.
2.2 Embolization procedure and observation indicators
28 New Zealand adult rabbits were classified as Group A and Group B. Group A (n=12) received right renal artery embolization with commercially available PVA particles, while Group B (n= 16) with PVA particles prepared in our laboratory. Su-mian-xin II was injected through ear edge vein for anesthesia in all animals. After successful anesthesia, an angiography catheter was inserted
体外毒性实验研究证实了自制PVA颗粒的安全性。以进口PVA颗粒作为对照组,采用介入导管栓塞技术行兔右肾动脉栓塞来考察栓塞效果。在影像学上应用CTA和DSA进行评价,在组织病理学上观察栓塞材料对栓塞动脉及肾实质的影响。研究结果表明:自制PVA颗粒具有较好的组织相容性,与进口PVA颗粒有相似的栓塞效果。所以自制PVA颗粒可作为血管栓塞剂,但在临床应用上还需进一步深入研究。
本实验研究首次应用CTA检查评价栓塞效果,证实了其与DSA具有相似的效果,为栓塞材料的研发找到了合适的、准确的评价方法;与此同时,也完善了导管栓塞技术,明确颈动脉插管是对股动脉插管失败后的一个有效的弥补方法。
Objective
To study the preparation methods and physical-chemical properties of PVA material; To assess the safety and embolic effect of self-made PVA particles as arterial embolic agent. To compare the efficacies of different imaging methods in evaluating embolic effect and summarize the experimental experiences of transcatheter renal arterial embolization in rabbits.
Materials and Methods
1 Laboratory preparation of self-made PVA material
We developed a method to prepare PVA absorbent material by mixing Polyvinylalcohol together with formaldehyde with rapid agitation. The surface density, multiplication after absorbing water, volume increase rate after absorbing water, climbing height after absorbing water and its morphological structure were measured.
2. Animal experiment of prepared PVA particles
2.1 Sterile test, fever-induced test, blood compatiblity, histocompatiblity test and toxic test were done for prepared PVA particles.
2.2 Embolization procedure and observation indicators
28 New Zealand adult rabbits were classified as Group A and Group B. Group A (n=12) received right renal artery embolization with commercially available PVA particles, while Group B (n= 16) with PVA particles prepared in our laboratory. Su-mian-xin II was injected through ear edge vein for anesthesia in all animals. After successful anesthesia, an angiography catheter was inserted
引文
1. 刘作勤. 经导管栓塞术. 医学影像学杂志, 2003,13(3):194-198.
2. 伍筱梅, 陈卫国, 赖清, 等. 肾动静脉瘘的选择性血管造影与栓塞治疗。中华泌尿外科杂志, 2003,24(5):296-299.
3. 范新东,张志愿,毛青,等. 颌面部先天性动静脉畸形的 PVA 栓塞治疗. 上海口腔医学,2000,9(4):200-202.
4. 何冰峰,赵锦龙,曹振远,等. PVA 栓塞支气管动脉治疗急性大咯血 12例疗效分析。中国急救医学,2002,22(3):183.
5. 杨继金,林琳,田建明,等. 不同材料栓塞甲状腺动脉治疗甲状腺功能亢进的疗效分析。介入放射学杂志, 2003,12(2):88-90.
6. Diaz-Daza O, Arraiza FJ, Barkley JM,et al. Endovascular therapy of traumatic vascular lesions of the head and neck. Cardiovasc Intervent Radiol,2003 26(3):213-221.
7. 曹华妹,虞希翔,陈伟. 经导管子宫动脉栓塞治疗子宫肌瘤的疗效观察。中国微创外科杂志, 2003,3(2):155-156.
8. 葛勤,刘同华,黄林清. 中药白芨作为血管栓塞剂及药物载体的研究概况。中国药房, 2003,14(5):305-307.
9. 华长春,马廉亭. 颅内动脉瘤血管内栓塞治疗材料的进展与评价。金陵医院学报,1998,11(3):257-260.
10. 朱刚,吴南,冯华,等. GDC 血管内栓塞治疗颅内动脉瘤。第三军医大学学报,2003,25(4):344-346.
11. 章翔,傅落安,吴景文,等. 机械可脱性微螺旋圈血管内栓塞治疗颅内动脉瘤。中华医学杂志, 2003,83(9):759-761.
12. 刘鸣,华续明,万亮,等. 微弹簧圈栓塞治疗急性破裂颅内动脉瘤的临床分析. 中华医学杂志, 2003,83(9):756-759.
13. 万杰清,李善泉,雄文浩,等. NBCA 胶栓塞治疗脑动静脉畸形。上海第二医科大学学报,2003,23(3):259-261.
14. 姜除寒,吴中学. 脑动脉畸形的非粘附性液体栓塞材料. 国外医学-脑血管疾病分册,2000,8(5):302-305.
15. 圣希忠,刘作勤. 非黏附性液体栓塞材料的研究进展. 医学影像学杂志,2004,14(10):852-854.
16. Sato A, Taki W. Ikada Y, et al, Experimental study and clinical use of poly(vinyl acetate) emulsion as liquid embolization material 。 Neuroradiology ,1994, 36:634-641.
17. Matsumaru Y,Hyodo A,Nose T,et al. Application of thermosensitive polyers as a new embolic material for intravascular neurosurgery. J biomater Sci Polym Ed, 1996,7:795-804.
18. 朱敬松,宋静,冯广森,等. 鸭胆子油动脉栓塞的动物实验研究. 医药论坛杂志,2004,25(23):54-55.
19. Kwak BK, Shim HJ, Han SM,et al. Chitin-based embolic materials in the renal artery of rabbits: pathologic evaluation of an absorbable particulate agent. Radiology,2005,236(1):151-158.
20. Kai Y, Hamada J, Morioka M, et al. The utility of the microcrystalline cellulose sphere as a particulate embolic agent: an experimental study. AJNR Am J Neuroradiol,2000,21(6):1160-1163.
21. Oowaki H, Matsuda S, Sakai N, et al. Non-adhesive cyanoacrylate as an embolic material for endovascular neurosurgery. Biomaterials , 2000, 21(10):1039-1046.
22. Jean-pierre Pelage, Alexandre Laurent, Michel Wassef, et al. Uterine artery embolization in sheep:comparison of acute effects with polyvinyl alcohol particles and calibrated microspheres. Radiology,2002,224(2):436-445.
23. 顾亚律,袁曙光,闫东,等. 四氧化三铁微粒与碘化油混悬液栓塞兔肾动脉的实验研究。中国介入影像与治疗学,2005,2(2):140-144.
24. Becker TA, Preul Mc, Bichard WD, et al. Calcium alginate gel as a biocompatible material for endovascular arteriovenous malformation embolization: six-month results in an animal model. Neurosurgery ,2005,56(4):793-801.
25. Chang D, Seo M, Choi H, et al. Application of transarterial embolization of renal artery in rabbits with experimental hydronephrosis. J Vet Med Sci,2002, 64(8):693-698.
26. Tino Tancredi, Patricia A, Mccuskey, et al. Changes in rat liver microcirculation after experimental hepatic arterial embolization:comparison of different embolic agents. Radiology,1999,211(1):177-181.
27. Komemushi A, Tangigawa N, Okuda Y, et al. A new liquid embolic material for liver tumors. Acta Radiol,2002,43(2):186-191.
1. Grindlay J.H , Clagett O.T. Plastic sponge prosthesis for use after pneumonectomy:preliminary report of experimental study. Proc.Staff Meet. Mayo Ciin,1949,24,1538-1539.
2. Hogeman KE, Gustafson G, Bjorlin G.. Ivalon surgical sponge used as temporary cover of experimental skin defects in rats: A preliminary report. Acta Chir Scand,1961,121:83-89.
3. Tadavarthy SM, Knight L, Ovitt TW, et al. Amplatz K .Therapeutic transcatheter arterial embolization. Radiology,1974,112(1):13-16.
4. KC Wright, JH Anderson, C Gianturco,et al. Partial splenic embolization using polyvinyl alcohol foam, dextran, Polystyrene, or silicone An experimental study in dogs. Radiology,1982;142:351-354.
5. VP Chuang, CC Tsai, CS Soo, et al. Experimental canine hepatic artery embolization with polyvinyl alcohol foam particles. Radiology,1982,145:21-25.
6. 邹英华,蒋学祥,彭勃,等. 国产 PVA 微球肝动脉栓塞的实验研究. 中华放射学杂志,1989;23(6):330-332.
7. 张宏文,倪才方,赵辉,等. 国产聚乙烯醇动脉栓塞的实验研究。医学影像学杂志,2004,14(11):936-939.
8. 张宏文,倪才方,冷德嵘. 国产聚乙烯醇树脂组织相容性的实验研究. 苏州大学学报(医学版),2005,25(1):60-63.
1. 毛立江,胡六洁,林东旭,等。表面润滑型聚乙烯醇水凝胶医用导管的力学性能研究.中国康复中心理论与实践,1999,5(4):145-149。
2. 严瑞暄。水溶性高分子. 北京化学工业出版社,1998,6。
3. 邹新禧。超强吸水剂. 北京化学工业出版社,2002:314-326。
4. 龙明策,王鹏,郑彤。高吸水树脂的合成及其应用。高分子材料科学与工程,2002,18(5):31-34。
5. 万福忠,陈昌镜,梁江辉,等。聚乙烯醇缩甲醛制备条件的研究.湖北化工,1996(2):21。
6. 钱志屏。泡沫塑料,1998。
7. 赵华,范瑞生。高吸水性聚合物的应用与发展。中国塑料,1999,13(4):11-14。
8. 阿茹娜,张佐光,仲伟虹。高吸水聚合物材料在防沙治沙中的应用。合成塑料及塑料,2002,19(2):37-41。
9. 林润雄,黄毓礼。吸水性树脂吸水性及保水性的研究。高分子材料科学与工程,1999,15(4):117-118。
10. 曾尤,王瑞清。多孔聚乙烯醇缩甲醛凝胶力学行为对含水量变化的敏感性.高分子学报,2005,(3):363-367。
11. 叶永观,林先核。聚乙烯醇缩甲醛泡沫塑料的研制. 福建化工,1996,(2):19-21。
1. Kusano S, Murata K, Ohuchi H, et al. Low-dose particulate polyvinylalcohol embolization in massive small artery intestinal hemorrhage. Experimental and clinical results. Invest Radiol, 1987, 22(5):388-392.
2. Wright KC, Anderson JH, Gianturco C, et al. Partial splenic embolization using polyvinyl alcohol foam, dextran, polystyrene, or silicone. Radiology, 1982, 142: 351-354.
3. Paradossi G, Cavalieri F, Chiessi E, et al. Poly(vinyl alcohol) as versatile biomaterial for potential biomedical applications.J Mater Sci Mater Med,2003, 14(8):687-691.
4. Kobayashi M, Oka M. Characterization of a polyvinyl alcohol-hydrogel artificial articular cartilage prepared by injection molding. J Biomater Sci Polym Ed, 2004,15(6):741-751.
5. Seabra AB, De Oliveira MG. Poly(vinyl alcohol) and poly(vinyl pyrrolidone) blended films for local nitric oxide release. Biomaterials ,2004,25(17):3773-3782
6. Wan WK, Campbell G, Zhang ZF, et al. Optimizing the tensile properties of polyvinyl alcohol hydrogel for the construction of a bioprosthetic heart valve stent. J biomed Mater Res, 2002, 63(6):854-861.
7. Oka M, Ushio K, Kumar P, et al. Development of artificial articular cartilage. Proc Inst Mech Eng [H], 2000, 214(1):59-68.
8. Hara Y, Matsuura T, Taketani F, et al. Biocompatibility of polyvinylalcohol gel as a vitreous substitute. Nippon Ganka Gakkai Zasshi, 1998 ,102(4):247-255.
9. Ryu RK, Omary RA, Sichlau MJ, et al. Comparison of pain after uterine artery embolization using tris-acryl gelatin microspheres versus polyvinyl alcohol particles. Cardiovasc Intervent Radiol, 2003,26(4):375-378.
10. Spies JB, Allison S, Flick P, et al. Spherical polyvinyl alcohol versus tris-acryl gelatin microspheres for uterine artery embolization for leiomyomas: results of a limited randomized comparative study. J Vasc Interv Radiol ,2005 ,16(11):1431-1437.
11. Basike A, Rand T, Lomoschitz F, et al. Trisacryl gelatin microspheres versus polyvinyl alcohol particles in the preoperative embolization of bone neoplasms. Cardiovasc Intervent Radiol,2004,27(5):495-502.
12. 杨继金,林琳,田建明,等. 不同材料栓塞甲状腺动脉治疗甲状腺功能亢进症的疗效分析。介入放射学杂志,2003,12(2):88-90.
13. .Chiesa AG, Hart WR. Uterine artery embolization of leiomyomas with trisacryl gelatin microspheres (TGM): pathologic features and comparison with polyvinyl alcohol emboli. Int J Gynecol Pathol, 2004,23(4):386-392.
14. Spies JB, Allison S, Flick P, et al. Polyvinyl alcohol particles and tris-acryl gelatin microspheres for uterine artery embolization for leiomyomas: results of a randomized comparative study. J Vasc Interv Radiol, 2004,15(8):793-800.
15. Ryu RK, Omary RA, Sichlau MJ, et al. Comparison of pain after uterine artery embolization using tris-acryl gelatin microspheres versus polyvinyl alcohol particles. J Vasc Interv Radiol, 2003,26(4):375-378.
16. Mihmanli I, Cantasdemir M, Kantarci F, et al. Percutaneous embolization in the management of intractable vaginal bleeding. Arch gynecol Obstet, 2001,264(4):211-214.
17. Kwak BK, Shim HJ, Han SM,et al. Chitin-based embolic materials in the renalartery of rabbits: pathologic evaluation of an absorbable particulate agent. Radiology,2005,236(1):151-158.
18. Link DP, Strandberg JD, Virmani R. Histopathologic appearance of arterial occlusions with hydrogel and polyvinyl alcohol embolic material in domestic swine. J Vasc Interv Radiol, 1996,7(6):897-905.
19. Portsman W, Wierny L, Warnke H, et al. Catheter closure of patent ductus arteriosis: 62 casestreated without thoracotomy. Radiol Clin N Amer, 1971, 9:203-218.
20. Tadavarthy SM, Knight L, Ovitt T, et al. Therapeutic transcatheter arterial embolization. Radiology,1974, 112:13-16.
21. Tadavarthy SM. Moller JH, Amplatz K. Polyvinyl alcohol (Ivalon)-a new embolic material. AJR, 1975, 125:609-616.
22. White RI, Strandberg JV, Cross CS, et al. Therapeutic embolization with long-term occluding agents and their effects on embolized tissues. Radiology, 1977, 125:677-687.
23. Castaneda-Zuniga WR, Sanchez R, Amplatz K. Experimental observations on short and long-term effects of arterial occlusion with Ivalon. Radiology, 1978,126:783-785.
24. Kubo M, Kuwayama N, Hirashima Y, et al. Hydroxyapatite ceramics as a particulate embolic material: report of the physical properties of the hydroxyapatite particles and the animal study. AJNR Am Neuroradiol, 2003, 24(8):1540-1544.
25. Germano IM, Davis RL, Wilson CB, et al. Histopathological follow-up study of 66 cerebral arteriovenous malformations after therapeutic embolization with polyvinyl alcohol. J Neurosurg, 1992 ,76(4):607-614.
26. Quisling RL, Mickle JP, Ballinger WB, et al. Histopathologic analysis of intraarterial polyvinyl alcohol microemboli in rat cerebral cortex. AJNR Am J Neuroradid, 1985,5:101-104.
27. George S, Karel G. Histologic long-term follow-up after embolization with polyvinyl alcohol particles. AJNR, 1995,16:843-846.
28. Kubo M, Kuwayama N, Hirashima Y, et al. Hydroxyapatite ceramics as a particulate embolic material: report of the physical properties of the hydroxyapatite particles and the animal study. AJNR Am J Neuroradiol, 2003, 24(8):1540-1544.
29. 姜兴岳,姚振威,沈天真,等.十六排螺旋 CT 血管造影对颅内病变的应用价值初探。CT 杂志,2003,6:20.
30. Tomandl BF,Kostner NC,Schempershofe M,et al. CT Angiography of intracranial aneurysms: a foucus on postprocessing. Radiographics , 2004,24(3):637-655.
31. Bader TR,Prokesch RW,Crabenwoger F. Timing of the hepatic arterial phase during contrast-enhanced computed tomography of the liver:assessment of normal values in 25 volunteers.Invest Radiol,2000,219:486-491.
32. Flelischmann D,Rubin GD,Bankier AA, et al.Improved uniformity of arotic enhancement with customized contrast medium injection protocols at CT angiography. Radiography,2000,214:363-371.
33. Gailloud P,Oishi S,Murphy K.Three-dimensional fusion digital subtraction angiography: new reconstruction algorithm for simultaneous three-dimensional rending of osseous and vascular information obtained during rotational angiography. AJNR Am J Neuroradiol,2005,26(4):908-911.
34. Choe DH, Han MH, Kang GH, et al. An experimental study of embolic effectaccording to infusion rate and concentration of suspension in transarterial particulate embolization. Invest Radiol, 1997, 32(5):260-267.
35. Imre Repa, Glenn , Moradian, et al. Mortalities Associated with Use of a Commercial Suspension of Polyvinyl Alcohol. Radiology,1989,170: 395-399.
36. Kjellin IB, Boechat MI, Vinuela F, et al. Pulmonary emboli following therapeutic embolization of cerebral arteriovenous malformations in children. Pediatr Radiol, 2000,30(40):279-283.
37. Tarak H Patel,Hideo Kimura,Clifford R,et al. Constitutively active HIF-1αimproves perfusion and arterial remolding in an endovascular model of limb ischemia. Cardiovascular Research,2005,68:144-154.
38. 支爱华,阎东,韩正林,等. 自制铜圈栓塞兔髂动脉及肾动脉的实验研究。中华放射学杂志,2003,37(3):205-209。
39. Kwak BK, Shim HJ, Han SM, et al. Chitin-based embolic materials in the renal artery of rabbits: pathologic evaluation of an absorbable particulate agent. Radiology, 2005,236(1):151-158.
40. Azzam A Khankan, Keigo Osuga, Shinichi Hori, et al.Embolic effects of superabsorbent polymer microspheres in rabbit renal model:comparison with tris-acryl gelatin microspheres and polyvinyl alcohol. Radiation Medicine, 2004,22(6):384-390.
2. 伍筱梅, 陈卫国, 赖清, 等. 肾动静脉瘘的选择性血管造影与栓塞治疗。中华泌尿外科杂志, 2003,24(5):296-299.
3. 范新东,张志愿,毛青,等. 颌面部先天性动静脉畸形的 PVA 栓塞治疗. 上海口腔医学,2000,9(4):200-202.
4. 何冰峰,赵锦龙,曹振远,等. PVA 栓塞支气管动脉治疗急性大咯血 12例疗效分析。中国急救医学,2002,22(3):183.
5. 杨继金,林琳,田建明,等. 不同材料栓塞甲状腺动脉治疗甲状腺功能亢进的疗效分析。介入放射学杂志, 2003,12(2):88-90.
6. Diaz-Daza O, Arraiza FJ, Barkley JM,et al. Endovascular therapy of traumatic vascular lesions of the head and neck. Cardiovasc Intervent Radiol,2003 26(3):213-221.
7. 曹华妹,虞希翔,陈伟. 经导管子宫动脉栓塞治疗子宫肌瘤的疗效观察。中国微创外科杂志, 2003,3(2):155-156.
8. 葛勤,刘同华,黄林清. 中药白芨作为血管栓塞剂及药物载体的研究概况。中国药房, 2003,14(5):305-307.
9. 华长春,马廉亭. 颅内动脉瘤血管内栓塞治疗材料的进展与评价。金陵医院学报,1998,11(3):257-260.
10. 朱刚,吴南,冯华,等. GDC 血管内栓塞治疗颅内动脉瘤。第三军医大学学报,2003,25(4):344-346.
11. 章翔,傅落安,吴景文,等. 机械可脱性微螺旋圈血管内栓塞治疗颅内动脉瘤。中华医学杂志, 2003,83(9):759-761.
12. 刘鸣,华续明,万亮,等. 微弹簧圈栓塞治疗急性破裂颅内动脉瘤的临床分析. 中华医学杂志, 2003,83(9):756-759.
13. 万杰清,李善泉,雄文浩,等. NBCA 胶栓塞治疗脑动静脉畸形。上海第二医科大学学报,2003,23(3):259-261.
14. 姜除寒,吴中学. 脑动脉畸形的非粘附性液体栓塞材料. 国外医学-脑血管疾病分册,2000,8(5):302-305.
15. 圣希忠,刘作勤. 非黏附性液体栓塞材料的研究进展. 医学影像学杂志,2004,14(10):852-854.
16. Sato A, Taki W. Ikada Y, et al, Experimental study and clinical use of poly(vinyl acetate) emulsion as liquid embolization material 。 Neuroradiology ,1994, 36:634-641.
17. Matsumaru Y,Hyodo A,Nose T,et al. Application of thermosensitive polyers as a new embolic material for intravascular neurosurgery. J biomater Sci Polym Ed, 1996,7:795-804.
18. 朱敬松,宋静,冯广森,等. 鸭胆子油动脉栓塞的动物实验研究. 医药论坛杂志,2004,25(23):54-55.
19. Kwak BK, Shim HJ, Han SM,et al. Chitin-based embolic materials in the renal artery of rabbits: pathologic evaluation of an absorbable particulate agent. Radiology,2005,236(1):151-158.
20. Kai Y, Hamada J, Morioka M, et al. The utility of the microcrystalline cellulose sphere as a particulate embolic agent: an experimental study. AJNR Am J Neuroradiol,2000,21(6):1160-1163.
21. Oowaki H, Matsuda S, Sakai N, et al. Non-adhesive cyanoacrylate as an embolic material for endovascular neurosurgery. Biomaterials , 2000, 21(10):1039-1046.
22. Jean-pierre Pelage, Alexandre Laurent, Michel Wassef, et al. Uterine artery embolization in sheep:comparison of acute effects with polyvinyl alcohol particles and calibrated microspheres. Radiology,2002,224(2):436-445.
23. 顾亚律,袁曙光,闫东,等. 四氧化三铁微粒与碘化油混悬液栓塞兔肾动脉的实验研究。中国介入影像与治疗学,2005,2(2):140-144.
24. Becker TA, Preul Mc, Bichard WD, et al. Calcium alginate gel as a biocompatible material for endovascular arteriovenous malformation embolization: six-month results in an animal model. Neurosurgery ,2005,56(4):793-801.
25. Chang D, Seo M, Choi H, et al. Application of transarterial embolization of renal artery in rabbits with experimental hydronephrosis. J Vet Med Sci,2002, 64(8):693-698.
26. Tino Tancredi, Patricia A, Mccuskey, et al. Changes in rat liver microcirculation after experimental hepatic arterial embolization:comparison of different embolic agents. Radiology,1999,211(1):177-181.
27. Komemushi A, Tangigawa N, Okuda Y, et al. A new liquid embolic material for liver tumors. Acta Radiol,2002,43(2):186-191.
1. Grindlay J.H , Clagett O.T. Plastic sponge prosthesis for use after pneumonectomy:preliminary report of experimental study. Proc.Staff Meet. Mayo Ciin,1949,24,1538-1539.
2. Hogeman KE, Gustafson G, Bjorlin G.. Ivalon surgical sponge used as temporary cover of experimental skin defects in rats: A preliminary report. Acta Chir Scand,1961,121:83-89.
3. Tadavarthy SM, Knight L, Ovitt TW, et al. Amplatz K .Therapeutic transcatheter arterial embolization. Radiology,1974,112(1):13-16.
4. KC Wright, JH Anderson, C Gianturco,et al. Partial splenic embolization using polyvinyl alcohol foam, dextran, Polystyrene, or silicone An experimental study in dogs. Radiology,1982;142:351-354.
5. VP Chuang, CC Tsai, CS Soo, et al. Experimental canine hepatic artery embolization with polyvinyl alcohol foam particles. Radiology,1982,145:21-25.
6. 邹英华,蒋学祥,彭勃,等. 国产 PVA 微球肝动脉栓塞的实验研究. 中华放射学杂志,1989;23(6):330-332.
7. 张宏文,倪才方,赵辉,等. 国产聚乙烯醇动脉栓塞的实验研究。医学影像学杂志,2004,14(11):936-939.
8. 张宏文,倪才方,冷德嵘. 国产聚乙烯醇树脂组织相容性的实验研究. 苏州大学学报(医学版),2005,25(1):60-63.
1. 毛立江,胡六洁,林东旭,等。表面润滑型聚乙烯醇水凝胶医用导管的力学性能研究.中国康复中心理论与实践,1999,5(4):145-149。
2. 严瑞暄。水溶性高分子. 北京化学工业出版社,1998,6。
3. 邹新禧。超强吸水剂. 北京化学工业出版社,2002:314-326。
4. 龙明策,王鹏,郑彤。高吸水树脂的合成及其应用。高分子材料科学与工程,2002,18(5):31-34。
5. 万福忠,陈昌镜,梁江辉,等。聚乙烯醇缩甲醛制备条件的研究.湖北化工,1996(2):21。
6. 钱志屏。泡沫塑料,1998。
7. 赵华,范瑞生。高吸水性聚合物的应用与发展。中国塑料,1999,13(4):11-14。
8. 阿茹娜,张佐光,仲伟虹。高吸水聚合物材料在防沙治沙中的应用。合成塑料及塑料,2002,19(2):37-41。
9. 林润雄,黄毓礼。吸水性树脂吸水性及保水性的研究。高分子材料科学与工程,1999,15(4):117-118。
10. 曾尤,王瑞清。多孔聚乙烯醇缩甲醛凝胶力学行为对含水量变化的敏感性.高分子学报,2005,(3):363-367。
11. 叶永观,林先核。聚乙烯醇缩甲醛泡沫塑料的研制. 福建化工,1996,(2):19-21。
1. Kusano S, Murata K, Ohuchi H, et al. Low-dose particulate polyvinylalcohol embolization in massive small artery intestinal hemorrhage. Experimental and clinical results. Invest Radiol, 1987, 22(5):388-392.
2. Wright KC, Anderson JH, Gianturco C, et al. Partial splenic embolization using polyvinyl alcohol foam, dextran, polystyrene, or silicone. Radiology, 1982, 142: 351-354.
3. Paradossi G, Cavalieri F, Chiessi E, et al. Poly(vinyl alcohol) as versatile biomaterial for potential biomedical applications.J Mater Sci Mater Med,2003, 14(8):687-691.
4. Kobayashi M, Oka M. Characterization of a polyvinyl alcohol-hydrogel artificial articular cartilage prepared by injection molding. J Biomater Sci Polym Ed, 2004,15(6):741-751.
5. Seabra AB, De Oliveira MG. Poly(vinyl alcohol) and poly(vinyl pyrrolidone) blended films for local nitric oxide release. Biomaterials ,2004,25(17):3773-3782
6. Wan WK, Campbell G, Zhang ZF, et al. Optimizing the tensile properties of polyvinyl alcohol hydrogel for the construction of a bioprosthetic heart valve stent. J biomed Mater Res, 2002, 63(6):854-861.
7. Oka M, Ushio K, Kumar P, et al. Development of artificial articular cartilage. Proc Inst Mech Eng [H], 2000, 214(1):59-68.
8. Hara Y, Matsuura T, Taketani F, et al. Biocompatibility of polyvinylalcohol gel as a vitreous substitute. Nippon Ganka Gakkai Zasshi, 1998 ,102(4):247-255.
9. Ryu RK, Omary RA, Sichlau MJ, et al. Comparison of pain after uterine artery embolization using tris-acryl gelatin microspheres versus polyvinyl alcohol particles. Cardiovasc Intervent Radiol, 2003,26(4):375-378.
10. Spies JB, Allison S, Flick P, et al. Spherical polyvinyl alcohol versus tris-acryl gelatin microspheres for uterine artery embolization for leiomyomas: results of a limited randomized comparative study. J Vasc Interv Radiol ,2005 ,16(11):1431-1437.
11. Basike A, Rand T, Lomoschitz F, et al. Trisacryl gelatin microspheres versus polyvinyl alcohol particles in the preoperative embolization of bone neoplasms. Cardiovasc Intervent Radiol,2004,27(5):495-502.
12. 杨继金,林琳,田建明,等. 不同材料栓塞甲状腺动脉治疗甲状腺功能亢进症的疗效分析。介入放射学杂志,2003,12(2):88-90.
13. .Chiesa AG, Hart WR. Uterine artery embolization of leiomyomas with trisacryl gelatin microspheres (TGM): pathologic features and comparison with polyvinyl alcohol emboli. Int J Gynecol Pathol, 2004,23(4):386-392.
14. Spies JB, Allison S, Flick P, et al. Polyvinyl alcohol particles and tris-acryl gelatin microspheres for uterine artery embolization for leiomyomas: results of a randomized comparative study. J Vasc Interv Radiol, 2004,15(8):793-800.
15. Ryu RK, Omary RA, Sichlau MJ, et al. Comparison of pain after uterine artery embolization using tris-acryl gelatin microspheres versus polyvinyl alcohol particles. J Vasc Interv Radiol, 2003,26(4):375-378.
16. Mihmanli I, Cantasdemir M, Kantarci F, et al. Percutaneous embolization in the management of intractable vaginal bleeding. Arch gynecol Obstet, 2001,264(4):211-214.
17. Kwak BK, Shim HJ, Han SM,et al. Chitin-based embolic materials in the renalartery of rabbits: pathologic evaluation of an absorbable particulate agent. Radiology,2005,236(1):151-158.
18. Link DP, Strandberg JD, Virmani R. Histopathologic appearance of arterial occlusions with hydrogel and polyvinyl alcohol embolic material in domestic swine. J Vasc Interv Radiol, 1996,7(6):897-905.
19. Portsman W, Wierny L, Warnke H, et al. Catheter closure of patent ductus arteriosis: 62 casestreated without thoracotomy. Radiol Clin N Amer, 1971, 9:203-218.
20. Tadavarthy SM, Knight L, Ovitt T, et al. Therapeutic transcatheter arterial embolization. Radiology,1974, 112:13-16.
21. Tadavarthy SM. Moller JH, Amplatz K. Polyvinyl alcohol (Ivalon)-a new embolic material. AJR, 1975, 125:609-616.
22. White RI, Strandberg JV, Cross CS, et al. Therapeutic embolization with long-term occluding agents and their effects on embolized tissues. Radiology, 1977, 125:677-687.
23. Castaneda-Zuniga WR, Sanchez R, Amplatz K. Experimental observations on short and long-term effects of arterial occlusion with Ivalon. Radiology, 1978,126:783-785.
24. Kubo M, Kuwayama N, Hirashima Y, et al. Hydroxyapatite ceramics as a particulate embolic material: report of the physical properties of the hydroxyapatite particles and the animal study. AJNR Am Neuroradiol, 2003, 24(8):1540-1544.
25. Germano IM, Davis RL, Wilson CB, et al. Histopathological follow-up study of 66 cerebral arteriovenous malformations after therapeutic embolization with polyvinyl alcohol. J Neurosurg, 1992 ,76(4):607-614.
26. Quisling RL, Mickle JP, Ballinger WB, et al. Histopathologic analysis of intraarterial polyvinyl alcohol microemboli in rat cerebral cortex. AJNR Am J Neuroradid, 1985,5:101-104.
27. George S, Karel G. Histologic long-term follow-up after embolization with polyvinyl alcohol particles. AJNR, 1995,16:843-846.
28. Kubo M, Kuwayama N, Hirashima Y, et al. Hydroxyapatite ceramics as a particulate embolic material: report of the physical properties of the hydroxyapatite particles and the animal study. AJNR Am J Neuroradiol, 2003, 24(8):1540-1544.
29. 姜兴岳,姚振威,沈天真,等.十六排螺旋 CT 血管造影对颅内病变的应用价值初探。CT 杂志,2003,6:20.
30. Tomandl BF,Kostner NC,Schempershofe M,et al. CT Angiography of intracranial aneurysms: a foucus on postprocessing. Radiographics , 2004,24(3):637-655.
31. Bader TR,Prokesch RW,Crabenwoger F. Timing of the hepatic arterial phase during contrast-enhanced computed tomography of the liver:assessment of normal values in 25 volunteers.Invest Radiol,2000,219:486-491.
32. Flelischmann D,Rubin GD,Bankier AA, et al.Improved uniformity of arotic enhancement with customized contrast medium injection protocols at CT angiography. Radiography,2000,214:363-371.
33. Gailloud P,Oishi S,Murphy K.Three-dimensional fusion digital subtraction angiography: new reconstruction algorithm for simultaneous three-dimensional rending of osseous and vascular information obtained during rotational angiography. AJNR Am J Neuroradiol,2005,26(4):908-911.
34. Choe DH, Han MH, Kang GH, et al. An experimental study of embolic effectaccording to infusion rate and concentration of suspension in transarterial particulate embolization. Invest Radiol, 1997, 32(5):260-267.
35. Imre Repa, Glenn , Moradian, et al. Mortalities Associated with Use of a Commercial Suspension of Polyvinyl Alcohol. Radiology,1989,170: 395-399.
36. Kjellin IB, Boechat MI, Vinuela F, et al. Pulmonary emboli following therapeutic embolization of cerebral arteriovenous malformations in children. Pediatr Radiol, 2000,30(40):279-283.
37. Tarak H Patel,Hideo Kimura,Clifford R,et al. Constitutively active HIF-1αimproves perfusion and arterial remolding in an endovascular model of limb ischemia. Cardiovascular Research,2005,68:144-154.
38. 支爱华,阎东,韩正林,等. 自制铜圈栓塞兔髂动脉及肾动脉的实验研究。中华放射学杂志,2003,37(3):205-209。
39. Kwak BK, Shim HJ, Han SM, et al. Chitin-based embolic materials in the renal artery of rabbits: pathologic evaluation of an absorbable particulate agent. Radiology, 2005,236(1):151-158.
40. Azzam A Khankan, Keigo Osuga, Shinichi Hori, et al.Embolic effects of superabsorbent polymer microspheres in rabbit renal model:comparison with tris-acryl gelatin microspheres and polyvinyl alcohol. Radiation Medicine, 2004,22(6):384-390.