阿魏菇深层发酵及其多糖的研究与应用
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摘要
本研究主要包括阿魏菇液体培养基的筛选、发酵条件的优化和胞内多糖提取及其理化性质的分析,相关功能饮料的研制四方面的内容,其结果如下: 1.通过单因子试验和正交试验,得到阿魏菇液体培养基(%)的最佳配比为:葡萄糖2,蔗糖2,淀粉1.3,麸皮2,黄豆2,KH2PO40.2,MgSO40.2,ZnSO40.2,NaCl0.2;2.液体摇瓶(250mL)发酵条件为:即31℃,150 r/min,初始PH值5.5,装液量125mL。初步获得了阿魏菇5L发酵罐深层发酵培养的发酵动力学曲线;3.胞内多糖最佳提取条件为,热水浸提料液比15,温度100℃,3h,提取3次,多糖分子量主要分布范围313.5万,59.0万,21.1万,3.4万,通过红外光谱仪分析其化学结构表明:阿魏菇多糖的化学结构是以β-糖苷键连接的、以吡喃葡萄糖为主;4.确定了两种不同剂型阿魏菇保健功能饮品的最佳配方和和工艺生产参数。
This paper mainly included four aspects: screening of liquid culture medium of Pleurotus ferulae Lanzi, optimization of fermentation conditions and extraction, analyses of physical and chemical properties of polysaccharide , the manufacture of the functional beverage,the results of which were as follows:
1. By monofactorial and orthogonal test, the optimal composition of liquid culture medium for the mycelia growth and the producing of intracelluar polysaccharide were respectively determined. The best culture medium(%) contained glucose2, sucrose2,starch1.3,bran2, soybean2,KH2PO40.2,MgSO40.2,ZnSO40.2,NaCl0.2。
2. The optimal flask (250ml) fermentation conditions were as follows: culture volume was 125mL, initial pH 5.5, culture temperature was 250℃, rotation speed was 150r/pm. And get the optimal jar (5L) fermentation conditions kinetics curve.
3. The optimal intracelluar polysaccharide extracting conditions are 15 times water ,100℃, 3h ,extract of polysaccharides 3 times, It’s molecular weight was estimated to be 313.5×104,59.0×104,21.1×104,3.4×104 by HPLC. Through UV spectrum, the structure of intracelluar polysaccharide is chiefly linked byβ-glycoside bonds and dominated by glucopyranose.
4. The optimum manufacture condition and technical parameters.for two kinds of Pleurotus ferulae Lenzi functional beverage were obtained by this research.
This paper mainly included four aspects: screening of liquid culture medium of Pleurotus ferulae Lanzi, optimization of fermentation conditions and extraction, analyses of physical and chemical properties of polysaccharide , the manufacture of the functional beverage,the results of which were as follows:
1. By monofactorial and orthogonal test, the optimal composition of liquid culture medium for the mycelia growth and the producing of intracelluar polysaccharide were respectively determined. The best culture medium(%) contained glucose2, sucrose2,starch1.3,bran2, soybean2,KH2PO40.2,MgSO40.2,ZnSO40.2,NaCl0.2。
2. The optimal flask (250ml) fermentation conditions were as follows: culture volume was 125mL, initial pH 5.5, culture temperature was 250℃, rotation speed was 150r/pm. And get the optimal jar (5L) fermentation conditions kinetics curve.
3. The optimal intracelluar polysaccharide extracting conditions are 15 times water ,100℃, 3h ,extract of polysaccharides 3 times, It’s molecular weight was estimated to be 313.5×104,59.0×104,21.1×104,3.4×104 by HPLC. Through UV spectrum, the structure of intracelluar polysaccharide is chiefly linked byβ-glycoside bonds and dominated by glucopyranose.
4. The optimum manufacture condition and technical parameters.for two kinds of Pleurotus ferulae Lenzi functional beverage were obtained by this research.
引文
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