LdMNPV不同地理品系致病力及荧光素作用的研究
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摘要
舞毒蛾核型多角体病毒( Lymantria dispar multiple embedded nucleopolyhedrovirus,LdMNPV)已成为控制舞毒蛾种群动态的重要生物因素之一。荧光素(Tinopal LPW)能够有效的提高LdMNPV对舞毒蛾幼虫的防治效果,寄主植物在利用LdMNPV防治舞毒蛾幼虫的过程中也有重要的作用。本文测定了Tinopal LPW---LdMNPV---寄主植物三者间的相互作用及对亚洲型舞毒蛾的影响。
为了更好的利用LdMNPV对亚洲型舞毒蛾幼虫进行生物防治,本文采用食料给毒法测定了不同地理品系LdMNPV对取食青杨的亚洲型舞毒蛾2龄幼虫的致死与亚致死作用。结果表明:LdMNPV-D、LdMNPV-H、LdMNPV-J 3个地理品系的LC_(50)分别为33、39、1076 OBs/μL,LT_(50)分别为10.5、13.5、8.9d;亚致死剂量的LdMNPV 3个地理品系对亚洲型舞毒蛾的发育历期、雌雄性比及蛹重均有一定影响,主要的表现为:发育历期延长、雌雄性比升高及蛹重增加等。
为提高LdMNPV防治效果,在室内采用食料给毒法测定了不同浓度的Tinopal LPW对LdMNPV不同地理品系的增效作用及光保护作用,并比较了荧光素对不同病毒品系致死中浓度(LC_(50))及致死中时间(LT_(50))的影响。结果表明病毒浓度相同时,LdMNPV 3个地理品系随着Tinopal LPW浓度的增加亚洲型舞毒蛾幼虫的死亡率也增高。1% Tinopal LPW对LdMNPV不同品系均有较强的增效作用,添加1% Tinopal LPW的LdMNPV D、H和J 3个品系对取食青杨的亚洲型舞毒蛾幼虫的致死中浓度(LC_(50))比不添加1% Tinopal LPW的LC_(50)分别降低了33、24和61倍。此外,1% Tinopal LPW使3个品系的致死中时间(LT_(50))分别缩短了2.9、5.3、1.2d。Tinopal LPW对LdMNPV D、H和J 3个品系均有光保护作用,添加1% Tinopal LPW后在距离30W紫外灯40cm下照射16h后,它们保持的毒力比未添加Tinopal LPW分别高1.8、2.6和1.8倍。
Lymantria dispar multiple embedded nucleopolyhedrovirus has one of the important factors on control the population trends of gypsy moth. The optical brightener can effectively improve the control effect of the LdMNPV to gypsy moth larvae. And the host plants have important role in use LdMNPV to prevent gypsy moth larvae. This study determined the relation of Tinopal LPW---LdMNPV---host plants and the influence to the Asian gypsy moth.
In order to use of the LdMNPV to Asian gypsy moth larvae to the biological control better, the study use the diet to poison method to determine the lethal effect and sublethal effect of different geographic strains LdMNPV, which to the 2nd instar Asian gypsy moth larvae feeding poplar (Populus cathayana). The results showed: The LC50s of LdMNPV-D, LdMNPV-H, LdMNPV-J were 33, 39 and 1076 OBs/μL and the LT50s of the three isolates were 10.5, 13.5 and 8.9d. The sublethal dose of three geography LdMNPV strains have a certain influence to the developmental time, the sex ratio and the pupal weight of Asian gypsy moth larvae. The sublethal dose of LdMNPV-H and LdMNPV-J prolonged the developmental time of Asian gypsy moth, and the total insects stage had significant difference with the contrast; LdMNPV-D also prolonged the developmental time of Asian gypsy moth but the total insects stage had no significant difference with the contrast. The sublethal dose of three geography LdMNPV strains reduced the sex ratio of the females than males. LdMNPV-D strains had significant difference with the pupal weight of female and male ones, but LdMNPV-J strains have highly significant difference with the pupal weight of the female ones. The sublethal dose of LdMNPV-H have no significant effects to the the pupal weight of the female and male ones.
In order to select the geographic isolates of gypsy moth multiple embedded nucleopolyhedrovirus (LdMNPV) and enhance their activities, the Asian gypsy moth 1st instar larvae were reared on artificial gypsy moth diet until just before inoculation. The 2nd instar larvae were inoculated using the diet plug method and then were transferred to poplar (Populus cathayana) leaves at laboratory. UV protection function of Tinopal LPW to geographic isolates of LdMNPV and its optimum concentration were tested. The virulence of the three LdMNPV isolates (LdMNPV-D, LdMNPV-H, LdMNPV-J) with and without optical brightener to the 2~(nd) instar Asian gypsy moth larvae were compared. The results showed out that when the virus concentrations were same but with the different concentration of optical brightener, the mortality of Asian gypsy moth larvae increased as the increasing of Tinopal LPW concentration, the higher the concentration, the higher the mortality. As a viral enhancer, 1% Tinopal LPW increased the virulence of 3 virus isolates to the 2~(nd) instar Asian gypsy moth larvae. The LC_(50)s of LdMNPV-D, LdMNPV-H, LdMNPV-J with 1% Tinopal LPW were 1.0, 1.6 and 17.6 OBs/μL, respectively. Whereas the LC_(50)s of them without 1%Tinopal LPW were 32.9, 39.0 and 1076.4 OBs/μL, respectively. The virulence of LdMNPV-D, LdMNPV-H, LdMNPV-J were increased 33, 24 and 61 times by adding 1%Tinopal LPW into the virus solution, respectively. In addition, the LT50s of the three isolates with 1%Tinopal LPW were 2.9d, 5.3d and 1.2d shorter than that of the virus without 1%Tinopal LPW. 1%Tinopal LPW had UV protection function to the LdMNPV isolates. After16 hours exposure to a 30W UV lamplight at a distance of 40cm , the original virulence of LdMNPV-D, LdMNPV-H, LdMNPV-J with 1% Tinopal LPW were 1.8, 2.6 and 1.8 times higher than that without 1% Tinopal LPW respectively.
为了更好的利用LdMNPV对亚洲型舞毒蛾幼虫进行生物防治,本文采用食料给毒法测定了不同地理品系LdMNPV对取食青杨的亚洲型舞毒蛾2龄幼虫的致死与亚致死作用。结果表明:LdMNPV-D、LdMNPV-H、LdMNPV-J 3个地理品系的LC_(50)分别为33、39、1076 OBs/μL,LT_(50)分别为10.5、13.5、8.9d;亚致死剂量的LdMNPV 3个地理品系对亚洲型舞毒蛾的发育历期、雌雄性比及蛹重均有一定影响,主要的表现为:发育历期延长、雌雄性比升高及蛹重增加等。
为提高LdMNPV防治效果,在室内采用食料给毒法测定了不同浓度的Tinopal LPW对LdMNPV不同地理品系的增效作用及光保护作用,并比较了荧光素对不同病毒品系致死中浓度(LC_(50))及致死中时间(LT_(50))的影响。结果表明病毒浓度相同时,LdMNPV 3个地理品系随着Tinopal LPW浓度的增加亚洲型舞毒蛾幼虫的死亡率也增高。1% Tinopal LPW对LdMNPV不同品系均有较强的增效作用,添加1% Tinopal LPW的LdMNPV D、H和J 3个品系对取食青杨的亚洲型舞毒蛾幼虫的致死中浓度(LC_(50))比不添加1% Tinopal LPW的LC_(50)分别降低了33、24和61倍。此外,1% Tinopal LPW使3个品系的致死中时间(LT_(50))分别缩短了2.9、5.3、1.2d。Tinopal LPW对LdMNPV D、H和J 3个品系均有光保护作用,添加1% Tinopal LPW后在距离30W紫外灯40cm下照射16h后,它们保持的毒力比未添加Tinopal LPW分别高1.8、2.6和1.8倍。
Lymantria dispar multiple embedded nucleopolyhedrovirus has one of the important factors on control the population trends of gypsy moth. The optical brightener can effectively improve the control effect of the LdMNPV to gypsy moth larvae. And the host plants have important role in use LdMNPV to prevent gypsy moth larvae. This study determined the relation of Tinopal LPW---LdMNPV---host plants and the influence to the Asian gypsy moth.
In order to use of the LdMNPV to Asian gypsy moth larvae to the biological control better, the study use the diet to poison method to determine the lethal effect and sublethal effect of different geographic strains LdMNPV, which to the 2nd instar Asian gypsy moth larvae feeding poplar (Populus cathayana). The results showed: The LC50s of LdMNPV-D, LdMNPV-H, LdMNPV-J were 33, 39 and 1076 OBs/μL and the LT50s of the three isolates were 10.5, 13.5 and 8.9d. The sublethal dose of three geography LdMNPV strains have a certain influence to the developmental time, the sex ratio and the pupal weight of Asian gypsy moth larvae. The sublethal dose of LdMNPV-H and LdMNPV-J prolonged the developmental time of Asian gypsy moth, and the total insects stage had significant difference with the contrast; LdMNPV-D also prolonged the developmental time of Asian gypsy moth but the total insects stage had no significant difference with the contrast. The sublethal dose of three geography LdMNPV strains reduced the sex ratio of the females than males. LdMNPV-D strains had significant difference with the pupal weight of female and male ones, but LdMNPV-J strains have highly significant difference with the pupal weight of the female ones. The sublethal dose of LdMNPV-H have no significant effects to the the pupal weight of the female and male ones.
In order to select the geographic isolates of gypsy moth multiple embedded nucleopolyhedrovirus (LdMNPV) and enhance their activities, the Asian gypsy moth 1st instar larvae were reared on artificial gypsy moth diet until just before inoculation. The 2nd instar larvae were inoculated using the diet plug method and then were transferred to poplar (Populus cathayana) leaves at laboratory. UV protection function of Tinopal LPW to geographic isolates of LdMNPV and its optimum concentration were tested. The virulence of the three LdMNPV isolates (LdMNPV-D, LdMNPV-H, LdMNPV-J) with and without optical brightener to the 2~(nd) instar Asian gypsy moth larvae were compared. The results showed out that when the virus concentrations were same but with the different concentration of optical brightener, the mortality of Asian gypsy moth larvae increased as the increasing of Tinopal LPW concentration, the higher the concentration, the higher the mortality. As a viral enhancer, 1% Tinopal LPW increased the virulence of 3 virus isolates to the 2~(nd) instar Asian gypsy moth larvae. The LC_(50)s of LdMNPV-D, LdMNPV-H, LdMNPV-J with 1% Tinopal LPW were 1.0, 1.6 and 17.6 OBs/μL, respectively. Whereas the LC_(50)s of them without 1%Tinopal LPW were 32.9, 39.0 and 1076.4 OBs/μL, respectively. The virulence of LdMNPV-D, LdMNPV-H, LdMNPV-J were increased 33, 24 and 61 times by adding 1%Tinopal LPW into the virus solution, respectively. In addition, the LT50s of the three isolates with 1%Tinopal LPW were 2.9d, 5.3d and 1.2d shorter than that of the virus without 1%Tinopal LPW. 1%Tinopal LPW had UV protection function to the LdMNPV isolates. After16 hours exposure to a 30W UV lamplight at a distance of 40cm , the original virulence of LdMNPV-D, LdMNPV-H, LdMNPV-J with 1% Tinopal LPW were 1.8, 2.6 and 1.8 times higher than that without 1% Tinopal LPW respectively.
引文
1 Pogue M G,Schaefer P W. A review of selected species of Lymantria (Hübner [1819]) (Lepidoptera: Noctuidae: Lymantriinae) from subtropical and temperate regions of Asia including the description of three new species, some potentially invasive to North America[M]. Fort Collins, Colorado: Publication FHTET 2006-2007, United States Department of Agriculture, Forest Service, Forest Health Technology Enterprise Team.
2兰星平,万志民等.舞毒蛾生物虚伪特性与繁殖技术的研究[J].贵州林业科技.1996,(4):1-6.
3徐建东,郑红军,袁朝仙等.舞毒蛾生物学特性及防治[J].贵州林业科技,2007,35(3): 17-19.
4杜松华.舞毒蛾在阿尔泰山林区的发生特点及防治措施[J].新疆林业,2004,(5):40.
5 Rubtsov I A. Effect of constant and fluctuating temperatures on egg development in the gypsy moth (Porthetria dispar)[J]. Zashchita Rasteniy,1938,17:25-38.
6 Abdullaev Y N. Biological characteristics of the gypsy moth in the forests of Samark and Province[J]. In: Voprosy zashchi rasteniy. Tashkent,1967,80-82.
7 Barteneva Y.V.,Gostjunin I.V.,Persidskaya L.T. The effect of temperature on the feeding rate of gypsy moth larvae[J]. In: Voprosy zashchity lesa. MLTI, Moskova, 1974. 93-96.
8刘雅娟,陶涛.浅谈舞毒蛾预测预报及防治对策[J].林业勘察设计. 2003, (2): 56-58.
9张执忠等.舞毒蛾综合管理的研究[J].北京:北京科学技术出版社,1988.
10钦俊德.植食性昆虫的食性和营养[J].昆虫学报.1962,11:169-185.
11 Kruse J J,Raffa K F. Effects of selected Midwestern larval host plants on performance by two strains of the gypsy moth (Lepidoptera Lymantriidae) parasitoid Cotesia melanoscela (HymenopteraBraconidae)[J]. Environmental Entomology. 1997, 26(5): 1155-1166.
12石娟,王月,徐洪儒,闫国增等.不同食料植物对舞毒蛾生长发育的影响[J].北京林业大学学报,2003,25(5):47-50.
13王丽珍.不同植物对舞毒蛾Lymantria dispar L.生物学特性的影响及其机理的研究. [D].2006,内蒙古农业大学硕士学位论文.
14朱丽春.寄主植物与病毒品系对舞毒蛾核型多角体病毒毒力的影响[D].2008,内蒙古农业大学硕士学位论文.
15杨高鹏.荧光增白剂、寄主植物、LdMNPV地理品系对舞毒蛾幼虫酚氧化酶和中肠酯酶的影响[D].2009,内蒙古农业大学硕士学位论文.
16朱丽春,段立清,特木欣等.舞毒蛾核型多角体病毒3个地理品系的毒力比较[J].中国森林病虫,2008,27(5):4-6.
17丁翠,马可.舞毒蛾核型多角体病毒亚致死剂量对舞毒蛾幼虫的影响[J].昆虫学报,1993,(3):272-275.
18 Jenny S,Judith H. The Ecology and Evolution of insect Baculoviruses[J]. Annu. Rev. Ecol. Evol. Syst.,2003,34:239-272.
19 Peng J,Zhong J,Granados R R. A baculovirus enhancin alters the permeability of a mucosal midgut peritrophic matrix from lepidopteran larvae[J]. Insect Physiol,1999,45:159-166.
20 Popham H R,Bischoff D S,Slavicek J M. Both Lymantria dispar nucleopolyhe nucleopoly- hedrovirus enhancin genes contribute to viral potency[J]. Virol., 2001. 75:(86)39- 48.
21 Carruthers W R,Cory J S,Entwistle P F. Recovery of pine beauty moth Panolis flammea nuclear polyhedrosis virus from pine foliage[J]. Invertebr. Pathol.,1988,52:27-32.
22 Thompson C G,Scott D W,Wickman B E. Long-term persistence of the nuclear polyhedrosis virus of the Douglas-fir tussock moth, Orgyia pseudotsugata (Lepidoptera: Lymantriidae), in forest soil[J]. Environ. Entomol.,1981,10:254-255.
23 Racmo B.黑绒茧蜂传布舞毒蛾核多角体病毒[J]. Entomophaya,1977,22(2):207-215.
24杨学军.舞毒蛾生物防治研究进展[J].陕西林业科技,1996,(1):68-73.
25 Duffey S S,Hoover K,Bonning B C,Hammock B D. The impact of host plant on the efficacy of baculoviruses[J]. Rev. Pest. Toxicol.,1995,3:137-275.
26 Farrar R R J,Ridgway R L. Host plant effects on the activity of selected nuclear polyhedrosis viruses against the corn earworm and beet armyworm (Lepidoptera: Noctuidae)[J]. Environ. Entomol.,2000,29:108-115.
27 Raymond B, Vanbergen A, Pearce I, Hartley SE, Cory JS, Hails RS. Host plant species can influence the fitness of herbivore pathogens: the winter moth and its nucleopolyhedrovirus[J]. Oecologia,2002,131:533-541.
28 Ali M I,Felton G W,Meade T,Young S Y. Influence of interspecific and intraspecific host plant variation on the susceptibility of heliothines to a baculovirus[J]. Biol. Control,1998,12:42-49.
29 Hoover K,Stout M J,Alaniz S A,Hammock B D,Duffey S S. Influence of induced plant defenses in cotton and tomato on the efficacy of baculoviruses on noctuid larvae[J]. Chem. Ecol.,1998b,24:253-271.
30 Hunter M D,Schultz J C. Induced plant defenses breached? Phytochemical induction protects an herbivore from disease[J]. Oecologia,1993,94:195-203.
31 Ali M I,Young S Y,Felton G W,McNew R W. Influence of the host plant on occluded virus productivity and lethal infectivity of a baculovirus[J]. Biol. Control,2002,81:158-165.
32 Lee K P. Ecological factors impacting on the nutritional biology of a generalist and a specialist caterpillar: effects of pathogen and plant structural compound on macronutrient balancing[J]. DPhil thesis. Univ. Oxford,2002,168.
33 Clayton D H,Wolfe N D. The adaptive significance of selfmedication[J]. Trends Ecol[J]. Evol,1993,8:60-63.
34 Lozano G A. Parasitic stress and selfmedication in wild animals[J]. Adv. Stud. Behav.,1998,27:291-317
35 Myers J H. Can a general hypothesis explain population cycles of forest Lepidoptera[J] Adv. Ecol. Res,1988,18:179-242.
36 Moscardi F. Assessment of the application of baculoviruses for control of Lepidoptera[J]. Annu. Rev. Entomol,1999,44:257-261.
37郭慧芳,方继朝,韩召军.昆虫病毒增效剂研究进展[J].昆虫学报,2003,46(6):766-772.
38 Rolinson W D,Lewis F B. Susceptibility of gypsy moth larvae to L ymant ria spp. Nuclear and cytoplassmic polyhedrosis virus[J]. Plant Protect,1973,24(124):163-168.
39 Shapiro M,Jacqueline L,Robertson.G. Comparative infectivities of gypsy moth (Lepidoptera: Lymantriidae) nucleopololyhedrosis virus isolates from North America, Europe, and Asia[J]. Econ. Entom,1984,77:153-156.
40 Paine C.,Radley J. A.,Rende L. P. Fabrics fluorescent to ultraviolet light[J]. U.S. patent,1937,2:408-413.
41 Eggert J,Wendt B. Wrapping materials[J]. U.S. patent,1939,2:171-174.
42 Lanter J. Properties and evaluation of fluorescent brightening agents[J]. Soc. Dyes CoJourists,1966,82:125-132.
43 Hollander H,Keilig W,Bauer J,RothemW1d E. A reliable fluorescent stain for fungi in tissue sections and clinical specimens[J]. Mycopathology,1984,88:131-134.
44 Nickle W R,Shapiro M. Use of a stilbene brightener, Tinopal LPW, as a radiation protectant for Steinemema carpocapsae[J]. Nematol.,1992,24:371--373.
45 Shapiro M.,Robertson J. L. Enhancement of gypsy moth (Lepidoptera: Lymantriidae) baculovirus activity by optical brighteners[J]. Econ. Entomol.,1992,85:1120-1124.
46 AnIiker R . History and mode of action of fluorescent whitening agents (FWA) In Fluorescent whitening agents[J]. Center for Environmental Sciences,1973,7-22.
47 Webb R E,Dill N H. Blankophor BBH as an enhancer of nuclear polyhedrosis virus in arborist treatments against the gypsy moth (Lepidoptera: Lymantriidae)[J]. Econ Entom.,1996,89(4):957-962.
48苏志坚,陈其津等.斜纹夜蛾核多角体病毒与增效剂混配对斜纹夜蛾幼虫的防治效果[J].中国生物防治, 2001, 17(1): 23-25.
49 Argauer R,Shapiro M. Fluorescence and relative activities of stilbene optical brighteners as enhancers for the gypsy moth (Lepidoptera: Lymantriidae) baculovirus[J]. Econ Entom.,1997,90(2):416-420.
50 Webb, R. E, Shapiro M, Podgwaite J D. Effect of optical brighteners on the efficacy of gypsy moth (Lepidoptera: Lymantriidae) nuclear polyhedrosis virus in forest plots with high or low levels of natural virus[J]. Econ. Entomol.,1994,87: 134-143.
51 Shapiro M,Argauer R. Effects of pH, temperature, and ultraviolet radiation on the activity of an optical brightener as a viral enhancer for the Gypsy Moth (Lepidoptera: Lymantriidae) baculovirus[J]. Econ. Entom.,1995,88(6):1602-1606.
52 Sheppard C A,Shapiro M. Physiological and nutritional effects of a fluorescent brightener on nuclear polyhedrosis virus-infected Lymantria dispar (L.) larvae (Lepidoptera: Lymantriidae)[J]. Biol. Control,1994,4: 404-411.
53 Shapiro M. Effect of two granulosis viruses on the activity of the gypsy moth (Lepidoptera: Lymantiidae) nuclear polyhedrosis virus[J]. Econ. Entomol.,2000b,93 (6):1633-1637.
54 Washburn J O,Kirkpatrick B A,Haas S E. Evidence that the stilbenederived optical brightener M2R enhances Autographa californica M nucleopolyhedrovirus infection of Trichoplusia ni and Heliothis virescens by preventing sloughing of infected midgut epithelial cells[J]. Biol. Control,1998,11:58-69.
55 Wang P,Granados R R. Calcofluor disrupts the midgut defense systemin insects[J]. Insect Biochem. Mol. Biol.,2000,30:135-143.
56黄茂福.荧光增白剂(一)[J].印染,2001,(4):37
57 Martignoni M.E.,Iwai P.J. Laboratory evaluation of new ultraviolet obsothers for protection of Douglas firo tussock moth (LePidoPtera: Lymantriidae) baculovinis [J]. Eeono. Eniomol,1985,78:982-987.
58严东辉,陈昌洁.提高昆虫病毒杀虫效果的荧光增白剂的研究[J].林业科学,2003,39(1):153-158.
59徐莉,杨红,彭建新等.荧光增白剂对苏云金芽抱杆菌毒力的增效作用及其紫外防护功效[J].中国生物防治,2001,17(2):63-66.
60 Dougherty EM,Guthrie KP,Shapiro M. Optical brighteners provide baculovirus activity enhancement and UV radiation protection[J]. Biological Control,1996,7: 71-74.
61马得强.荧光增白剂.见:化工百科全书编委会.化工百科全书[M].第18卷.第1版.北京:化学工业出版社,1998:1047-1062
62 Goulson D,Martinez A M,Hughes WO H et al. Effects of optical brighteners used in biopesticide formulations on the behavior of pollinators[J]. Biological Control,2000,19:232-236
63沈永嘉,李红斌,路炜.荧光增白剂[M].北京:化学工业出版社,2003,313-349
64 LeOra software. POLO-PC: a user's guide to probit or logit analysis, Berkeley, California: LeOra Software,1994.
65张文彤. SPSS11统计分析教程(高级篇)[M].北京:北京希望电子出版社,2002,119-123.
66段立清.昆虫病毒对鳞翅目食叶害虫的亚致死作用[J].中国森林病虫,2002,21(3):33-35
67徐林波.舞毒蛾核型多角体病毒(LdNPV)的不同地理品系对亚洲型舞毒蛾的致死与亚致死作用[D]. 2005.内蒙古农业大学硕士学位论文.
68 Ebling P.M.,Otvos I.S.,Conder N.. Comparative activity of three isolates of LdMNPV against two strains of Lymantria dispar[J]. Canadian entomologist , 2004 ,136(5):737-747.
69 Duan L Q, Otvos I S. Influence of larval age and virus concentration on mortality and sublethal effects of Nucleopoly hedrovirus on the Western spruce budworm (Lepidoptera: Tortrieidae)[J]. Environ entom, 2001, 30:136-146.
70席景会,潘洪玉,刘伟成等.八字地老虎核型多角体病毒对宿主的弱化作用[J].昆虫天敌,2000,22(2):75-78.
71 Rothman L D,Myers J H. Nuclear polyhedrosis virus treatment effect on reproductive potential of western tent caterpillar (Lepidoptera: Lasiocampidae)[J]. Environ Entomol,1994,23: 864-869.
72 Milks M L,Burnstyn I,Myers J H. Influence of larval age on the lethal and sublethal effects of the nucleopoly hedrovirus of Trichoplusia ni in the cabbage looper[J]. Biol Control,1998,12:119-126.
73 Boots M,Begon M. Resource limitation and the lethal and sublethal effects of avirus pathogen in the Indian meal moth, Plodia interpunctella[J]. Ecol Entomol,1994,19:319-329.
74周建华,胡应之等.荧光增白剂Tinopal LPW对蜀柏毒蛾核型多角体病毒增效作用研究[J].中国病毒学,2006.21(3):273-276. 32
75张丽红,彭建新等.荧光增白剂对重组苜蓿银纹夜蛾核型多角体病毒的增效作用[J].应用与环境生物学报,2004.10(5):609-613.
76杨高鹏,段立清等.荧光增白剂、寄主植物、LdNPV地理品系对舞毒蛾幼虫酚氧化酶的影响[J]林业科学,2010,46(9):105-109.
2兰星平,万志民等.舞毒蛾生物虚伪特性与繁殖技术的研究[J].贵州林业科技.1996,(4):1-6.
3徐建东,郑红军,袁朝仙等.舞毒蛾生物学特性及防治[J].贵州林业科技,2007,35(3): 17-19.
4杜松华.舞毒蛾在阿尔泰山林区的发生特点及防治措施[J].新疆林业,2004,(5):40.
5 Rubtsov I A. Effect of constant and fluctuating temperatures on egg development in the gypsy moth (Porthetria dispar)[J]. Zashchita Rasteniy,1938,17:25-38.
6 Abdullaev Y N. Biological characteristics of the gypsy moth in the forests of Samark and Province[J]. In: Voprosy zashchi rasteniy. Tashkent,1967,80-82.
7 Barteneva Y.V.,Gostjunin I.V.,Persidskaya L.T. The effect of temperature on the feeding rate of gypsy moth larvae[J]. In: Voprosy zashchity lesa. MLTI, Moskova, 1974. 93-96.
8刘雅娟,陶涛.浅谈舞毒蛾预测预报及防治对策[J].林业勘察设计. 2003, (2): 56-58.
9张执忠等.舞毒蛾综合管理的研究[J].北京:北京科学技术出版社,1988.
10钦俊德.植食性昆虫的食性和营养[J].昆虫学报.1962,11:169-185.
11 Kruse J J,Raffa K F. Effects of selected Midwestern larval host plants on performance by two strains of the gypsy moth (Lepidoptera Lymantriidae) parasitoid Cotesia melanoscela (HymenopteraBraconidae)[J]. Environmental Entomology. 1997, 26(5): 1155-1166.
12石娟,王月,徐洪儒,闫国增等.不同食料植物对舞毒蛾生长发育的影响[J].北京林业大学学报,2003,25(5):47-50.
13王丽珍.不同植物对舞毒蛾Lymantria dispar L.生物学特性的影响及其机理的研究. [D].2006,内蒙古农业大学硕士学位论文.
14朱丽春.寄主植物与病毒品系对舞毒蛾核型多角体病毒毒力的影响[D].2008,内蒙古农业大学硕士学位论文.
15杨高鹏.荧光增白剂、寄主植物、LdMNPV地理品系对舞毒蛾幼虫酚氧化酶和中肠酯酶的影响[D].2009,内蒙古农业大学硕士学位论文.
16朱丽春,段立清,特木欣等.舞毒蛾核型多角体病毒3个地理品系的毒力比较[J].中国森林病虫,2008,27(5):4-6.
17丁翠,马可.舞毒蛾核型多角体病毒亚致死剂量对舞毒蛾幼虫的影响[J].昆虫学报,1993,(3):272-275.
18 Jenny S,Judith H. The Ecology and Evolution of insect Baculoviruses[J]. Annu. Rev. Ecol. Evol. Syst.,2003,34:239-272.
19 Peng J,Zhong J,Granados R R. A baculovirus enhancin alters the permeability of a mucosal midgut peritrophic matrix from lepidopteran larvae[J]. Insect Physiol,1999,45:159-166.
20 Popham H R,Bischoff D S,Slavicek J M. Both Lymantria dispar nucleopolyhe nucleopoly- hedrovirus enhancin genes contribute to viral potency[J]. Virol., 2001. 75:(86)39- 48.
21 Carruthers W R,Cory J S,Entwistle P F. Recovery of pine beauty moth Panolis flammea nuclear polyhedrosis virus from pine foliage[J]. Invertebr. Pathol.,1988,52:27-32.
22 Thompson C G,Scott D W,Wickman B E. Long-term persistence of the nuclear polyhedrosis virus of the Douglas-fir tussock moth, Orgyia pseudotsugata (Lepidoptera: Lymantriidae), in forest soil[J]. Environ. Entomol.,1981,10:254-255.
23 Racmo B.黑绒茧蜂传布舞毒蛾核多角体病毒[J]. Entomophaya,1977,22(2):207-215.
24杨学军.舞毒蛾生物防治研究进展[J].陕西林业科技,1996,(1):68-73.
25 Duffey S S,Hoover K,Bonning B C,Hammock B D. The impact of host plant on the efficacy of baculoviruses[J]. Rev. Pest. Toxicol.,1995,3:137-275.
26 Farrar R R J,Ridgway R L. Host plant effects on the activity of selected nuclear polyhedrosis viruses against the corn earworm and beet armyworm (Lepidoptera: Noctuidae)[J]. Environ. Entomol.,2000,29:108-115.
27 Raymond B, Vanbergen A, Pearce I, Hartley SE, Cory JS, Hails RS. Host plant species can influence the fitness of herbivore pathogens: the winter moth and its nucleopolyhedrovirus[J]. Oecologia,2002,131:533-541.
28 Ali M I,Felton G W,Meade T,Young S Y. Influence of interspecific and intraspecific host plant variation on the susceptibility of heliothines to a baculovirus[J]. Biol. Control,1998,12:42-49.
29 Hoover K,Stout M J,Alaniz S A,Hammock B D,Duffey S S. Influence of induced plant defenses in cotton and tomato on the efficacy of baculoviruses on noctuid larvae[J]. Chem. Ecol.,1998b,24:253-271.
30 Hunter M D,Schultz J C. Induced plant defenses breached? Phytochemical induction protects an herbivore from disease[J]. Oecologia,1993,94:195-203.
31 Ali M I,Young S Y,Felton G W,McNew R W. Influence of the host plant on occluded virus productivity and lethal infectivity of a baculovirus[J]. Biol. Control,2002,81:158-165.
32 Lee K P. Ecological factors impacting on the nutritional biology of a generalist and a specialist caterpillar: effects of pathogen and plant structural compound on macronutrient balancing[J]. DPhil thesis. Univ. Oxford,2002,168.
33 Clayton D H,Wolfe N D. The adaptive significance of selfmedication[J]. Trends Ecol[J]. Evol,1993,8:60-63.
34 Lozano G A. Parasitic stress and selfmedication in wild animals[J]. Adv. Stud. Behav.,1998,27:291-317
35 Myers J H. Can a general hypothesis explain population cycles of forest Lepidoptera[J] Adv. Ecol. Res,1988,18:179-242.
36 Moscardi F. Assessment of the application of baculoviruses for control of Lepidoptera[J]. Annu. Rev. Entomol,1999,44:257-261.
37郭慧芳,方继朝,韩召军.昆虫病毒增效剂研究进展[J].昆虫学报,2003,46(6):766-772.
38 Rolinson W D,Lewis F B. Susceptibility of gypsy moth larvae to L ymant ria spp. Nuclear and cytoplassmic polyhedrosis virus[J]. Plant Protect,1973,24(124):163-168.
39 Shapiro M,Jacqueline L,Robertson.G. Comparative infectivities of gypsy moth (Lepidoptera: Lymantriidae) nucleopololyhedrosis virus isolates from North America, Europe, and Asia[J]. Econ. Entom,1984,77:153-156.
40 Paine C.,Radley J. A.,Rende L. P. Fabrics fluorescent to ultraviolet light[J]. U.S. patent,1937,2:408-413.
41 Eggert J,Wendt B. Wrapping materials[J]. U.S. patent,1939,2:171-174.
42 Lanter J. Properties and evaluation of fluorescent brightening agents[J]. Soc. Dyes CoJourists,1966,82:125-132.
43 Hollander H,Keilig W,Bauer J,RothemW1d E. A reliable fluorescent stain for fungi in tissue sections and clinical specimens[J]. Mycopathology,1984,88:131-134.
44 Nickle W R,Shapiro M. Use of a stilbene brightener, Tinopal LPW, as a radiation protectant for Steinemema carpocapsae[J]. Nematol.,1992,24:371--373.
45 Shapiro M.,Robertson J. L. Enhancement of gypsy moth (Lepidoptera: Lymantriidae) baculovirus activity by optical brighteners[J]. Econ. Entomol.,1992,85:1120-1124.
46 AnIiker R . History and mode of action of fluorescent whitening agents (FWA) In Fluorescent whitening agents[J]. Center for Environmental Sciences,1973,7-22.
47 Webb R E,Dill N H. Blankophor BBH as an enhancer of nuclear polyhedrosis virus in arborist treatments against the gypsy moth (Lepidoptera: Lymantriidae)[J]. Econ Entom.,1996,89(4):957-962.
48苏志坚,陈其津等.斜纹夜蛾核多角体病毒与增效剂混配对斜纹夜蛾幼虫的防治效果[J].中国生物防治, 2001, 17(1): 23-25.
49 Argauer R,Shapiro M. Fluorescence and relative activities of stilbene optical brighteners as enhancers for the gypsy moth (Lepidoptera: Lymantriidae) baculovirus[J]. Econ Entom.,1997,90(2):416-420.
50 Webb, R. E, Shapiro M, Podgwaite J D. Effect of optical brighteners on the efficacy of gypsy moth (Lepidoptera: Lymantriidae) nuclear polyhedrosis virus in forest plots with high or low levels of natural virus[J]. Econ. Entomol.,1994,87: 134-143.
51 Shapiro M,Argauer R. Effects of pH, temperature, and ultraviolet radiation on the activity of an optical brightener as a viral enhancer for the Gypsy Moth (Lepidoptera: Lymantriidae) baculovirus[J]. Econ. Entom.,1995,88(6):1602-1606.
52 Sheppard C A,Shapiro M. Physiological and nutritional effects of a fluorescent brightener on nuclear polyhedrosis virus-infected Lymantria dispar (L.) larvae (Lepidoptera: Lymantriidae)[J]. Biol. Control,1994,4: 404-411.
53 Shapiro M. Effect of two granulosis viruses on the activity of the gypsy moth (Lepidoptera: Lymantiidae) nuclear polyhedrosis virus[J]. Econ. Entomol.,2000b,93 (6):1633-1637.
54 Washburn J O,Kirkpatrick B A,Haas S E. Evidence that the stilbenederived optical brightener M2R enhances Autographa californica M nucleopolyhedrovirus infection of Trichoplusia ni and Heliothis virescens by preventing sloughing of infected midgut epithelial cells[J]. Biol. Control,1998,11:58-69.
55 Wang P,Granados R R. Calcofluor disrupts the midgut defense systemin insects[J]. Insect Biochem. Mol. Biol.,2000,30:135-143.
56黄茂福.荧光增白剂(一)[J].印染,2001,(4):37
57 Martignoni M.E.,Iwai P.J. Laboratory evaluation of new ultraviolet obsothers for protection of Douglas firo tussock moth (LePidoPtera: Lymantriidae) baculovinis [J]. Eeono. Eniomol,1985,78:982-987.
58严东辉,陈昌洁.提高昆虫病毒杀虫效果的荧光增白剂的研究[J].林业科学,2003,39(1):153-158.
59徐莉,杨红,彭建新等.荧光增白剂对苏云金芽抱杆菌毒力的增效作用及其紫外防护功效[J].中国生物防治,2001,17(2):63-66.
60 Dougherty EM,Guthrie KP,Shapiro M. Optical brighteners provide baculovirus activity enhancement and UV radiation protection[J]. Biological Control,1996,7: 71-74.
61马得强.荧光增白剂.见:化工百科全书编委会.化工百科全书[M].第18卷.第1版.北京:化学工业出版社,1998:1047-1062
62 Goulson D,Martinez A M,Hughes WO H et al. Effects of optical brighteners used in biopesticide formulations on the behavior of pollinators[J]. Biological Control,2000,19:232-236
63沈永嘉,李红斌,路炜.荧光增白剂[M].北京:化学工业出版社,2003,313-349
64 LeOra software. POLO-PC: a user's guide to probit or logit analysis, Berkeley, California: LeOra Software,1994.
65张文彤. SPSS11统计分析教程(高级篇)[M].北京:北京希望电子出版社,2002,119-123.
66段立清.昆虫病毒对鳞翅目食叶害虫的亚致死作用[J].中国森林病虫,2002,21(3):33-35
67徐林波.舞毒蛾核型多角体病毒(LdNPV)的不同地理品系对亚洲型舞毒蛾的致死与亚致死作用[D]. 2005.内蒙古农业大学硕士学位论文.
68 Ebling P.M.,Otvos I.S.,Conder N.. Comparative activity of three isolates of LdMNPV against two strains of Lymantria dispar[J]. Canadian entomologist , 2004 ,136(5):737-747.
69 Duan L Q, Otvos I S. Influence of larval age and virus concentration on mortality and sublethal effects of Nucleopoly hedrovirus on the Western spruce budworm (Lepidoptera: Tortrieidae)[J]. Environ entom, 2001, 30:136-146.
70席景会,潘洪玉,刘伟成等.八字地老虎核型多角体病毒对宿主的弱化作用[J].昆虫天敌,2000,22(2):75-78.
71 Rothman L D,Myers J H. Nuclear polyhedrosis virus treatment effect on reproductive potential of western tent caterpillar (Lepidoptera: Lasiocampidae)[J]. Environ Entomol,1994,23: 864-869.
72 Milks M L,Burnstyn I,Myers J H. Influence of larval age on the lethal and sublethal effects of the nucleopoly hedrovirus of Trichoplusia ni in the cabbage looper[J]. Biol Control,1998,12:119-126.
73 Boots M,Begon M. Resource limitation and the lethal and sublethal effects of avirus pathogen in the Indian meal moth, Plodia interpunctella[J]. Ecol Entomol,1994,19:319-329.
74周建华,胡应之等.荧光增白剂Tinopal LPW对蜀柏毒蛾核型多角体病毒增效作用研究[J].中国病毒学,2006.21(3):273-276. 32
75张丽红,彭建新等.荧光增白剂对重组苜蓿银纹夜蛾核型多角体病毒的增效作用[J].应用与环境生物学报,2004.10(5):609-613.
76杨高鹏,段立清等.荧光增白剂、寄主植物、LdNPV地理品系对舞毒蛾幼虫酚氧化酶的影响[J]林业科学,2010,46(9):105-109.