中药广藿香化学及生物活性成分研究
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摘要
本论文在综述中药广藿香(Pogostemon cablin(Blanco)Benth.)的药品,广藿香的资源以及广藿香在化学成分和药理活性等研究的基础上,对中药广藿香(P.cablinBenth.)乙醇提取物的氯仿乙酸乙酯萃取部位的化学成分进行了系统研究,并对各萃取部位和部分单体化合物进行多种生物活性筛选模型的测试。
应用多种分离材料和分离技术,结合现代波谱技术,从广藿香(P.cablin Benth.)乙醇提取物的氯仿乙酸乙酯萃取部位中分离并鉴定了21个化合物。化合物结构类型涉及黄酮、黄酮甙、甾体和三萜等,其中有3个化合物(化合物14,19和24)为首次从该属植物中分离,有10个化合物(化合物1,5,6,7,8,9,11,15,18和20)为首次从该植物中分离。
在化学成分研究的基础上,还对广藿香乙醇提取物的四个萃取部位(即石油醚部位、乙酸乙酯部位、正丁醇部位和水部位)及所得到的部分单体化合物进行了体外抗菌,抗肿瘤(人白血病细胞株K562)和α-葡萄糖苷酶抑制活性等方面的生物活性筛选。结果显示化合物10对枯草芽孢杆菌、大肠埃希氏菌及伤寒沙门氏菌具有一定的抑制作用;对人白血病细胞株K562无明显抑制作用;石油醚部位、正丁醇部位和水部位对α-葡萄糖苷酶有极微弱抑制作用,乙酸乙酯部位对α-葡萄糖苷酶有微弱的抑制作用;正在对化合物14,19和24进行抗氧化方面的活性筛选。
This thesis reviewed traditional Chinese medicine compounds,sources and chemical constituents,and pharmacological activities of the traditional medicine,Pogostemon cablin Benth.On basis of the advances,we further study on the chemical constituents and their pharmacological activities of involatility moiety of Pogostemon cablin Benth.
This thesis elaborated chemical constituents of Pogostemon cablin(Blanco) Benth. and bioassay on some extracts and partial compounds isolated.By variety of chromatographies over various materials,twenty-one compounds were isolated,which covered flavones,flavone glycosides,phenylpropanoid glycosides,steroids and triterpenoid.Their structures were elucidated major on the basis of spectroscopic methods.Three of them(compounds 14,19,and 24) were isolate from the Pogostemon genus for the first time,and nine of them(compounds 1,5,6,7,8,9,11,15,18,and 20) were isolated from the plant P.cablin Benth.for the first time.
Furthermore,the activities on antibacterial,α-D-glucoside glucohydrolase inhibitor and antitumor of some compounds were tested.As a result,compound 10 exhibited thin inhibition aganist Bacillus subtilis,Escherichia coli,and Salmonella typhi.The activities onα-D-glucoside glucohydrolase inhibitor and antitumor is negative.And the antioxidant bioactivity of three phenylpropanoid glycosides(compounds 14,19,and 24) are being tested.
应用多种分离材料和分离技术,结合现代波谱技术,从广藿香(P.cablin Benth.)乙醇提取物的氯仿乙酸乙酯萃取部位中分离并鉴定了21个化合物。化合物结构类型涉及黄酮、黄酮甙、甾体和三萜等,其中有3个化合物(化合物14,19和24)为首次从该属植物中分离,有10个化合物(化合物1,5,6,7,8,9,11,15,18和20)为首次从该植物中分离。
在化学成分研究的基础上,还对广藿香乙醇提取物的四个萃取部位(即石油醚部位、乙酸乙酯部位、正丁醇部位和水部位)及所得到的部分单体化合物进行了体外抗菌,抗肿瘤(人白血病细胞株K562)和α-葡萄糖苷酶抑制活性等方面的生物活性筛选。结果显示化合物10对枯草芽孢杆菌、大肠埃希氏菌及伤寒沙门氏菌具有一定的抑制作用;对人白血病细胞株K562无明显抑制作用;石油醚部位、正丁醇部位和水部位对α-葡萄糖苷酶有极微弱抑制作用,乙酸乙酯部位对α-葡萄糖苷酶有微弱的抑制作用;正在对化合物14,19和24进行抗氧化方面的活性筛选。
This thesis reviewed traditional Chinese medicine compounds,sources and chemical constituents,and pharmacological activities of the traditional medicine,Pogostemon cablin Benth.On basis of the advances,we further study on the chemical constituents and their pharmacological activities of involatility moiety of Pogostemon cablin Benth.
This thesis elaborated chemical constituents of Pogostemon cablin(Blanco) Benth. and bioassay on some extracts and partial compounds isolated.By variety of chromatographies over various materials,twenty-one compounds were isolated,which covered flavones,flavone glycosides,phenylpropanoid glycosides,steroids and triterpenoid.Their structures were elucidated major on the basis of spectroscopic methods.Three of them(compounds 14,19,and 24) were isolate from the Pogostemon genus for the first time,and nine of them(compounds 1,5,6,7,8,9,11,15,18,and 20) were isolated from the plant P.cablin Benth.for the first time.
Furthermore,the activities on antibacterial,α-D-glucoside glucohydrolase inhibitor and antitumor of some compounds were tested.As a result,compound 10 exhibited thin inhibition aganist Bacillus subtilis,Escherichia coli,and Salmonella typhi.The activities onα-D-glucoside glucohydrolase inhibitor and antitumor is negative.And the antioxidant bioactivity of three phenylpropanoid glycosides(compounds 14,19,and 24) are being tested.
引文
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3.冯毅凡,郭晓玲,罗集鹏.雷州产广藿香挥发油成分的气相色谱-质谱分析[J].中药材,1999,22(5):241-243.
4.包丽芹,韩德权.芹菜及芹菜黄酮的药用价值[J].黑龙江医药,2007,20(4):317-320.
5.任守忠,靳德军,张俊清,刘明生.广藿香药理作用研究进展[J].中国现代中药,2006,8(8):27-29.
6.关玲,权丽辉,丛浦珠.广藿香挥发油化学成份的研究[J].天然产物研究与开发,1992,4(2):34-37.
7.关玲,权丽辉,徐丽珍,从浦珠.广藿香化学成分的研究[J].中国中药杂志,1994,19(6):355-356.
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