中药茵陈承气汤对重症急性胰腺炎大鼠凝血功能的影响
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摘要
目的:观察中药茵陈承气汤对重症急性胰腺炎大鼠凝血功能的影响,为中药治疗重症急性胰腺炎(severe acute pancreatitis,SAP)提供理论依据及新的治疗思路。
方法:健康SD雄性大鼠30只,随机分为三组:对照组;模型组;中药治疗组(简称中药组);每组10只。模型建立的步骤:(1)麻醉:用4%的戊巴比妥钠按1ml/kg体重的剂量经腹腔注射麻醉。(2)麻醉生效后,固定大鼠于手术板上,取仰卧位,剪去腹部鼠毛,常规消毒,铺巾准备手术。(3)取上腹正中切口3cm左右,在严格无菌操作下打开腹腔,找到胃十二指肠交界处,再向下寻找到胰胆管十二指肠开口处。(4)用动脉夹夹闭肝总管,将4.5号针头平面朝上于胰胆管十二指肠开口处行被膜下穿刺,并向胰管方向推进约2-3mm,按1ml/kg体重将1%去氧胆酸钠逆行推注至胰管中(0.2ml/min),使整个胰胆管均匀隆起,退出针头,用手压迫进针处约3-5min,然后去除动脉夹,关腹,并同时开始计时观察。对照组为假手术组;模型组采用经胰胆管向大鼠胰腺组织内逆行注射1%去氧胆酸钠的方法诱导大鼠重症急性胰腺炎(SAP),中药组采用与模型组相同的方法制造SAP大鼠模型,并分别于造模前12h和造模后12h经口灌服中药茵陈承气汤水煎药液2ml/g。造模24h后采血,化验血液凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(FIB)及国际标准化比率(INR),对各组大鼠化验结果进行两两比较。
结果:
2.1 PT:模型组PT轻度延长,与对照组比较差异不显著,不具有统计学意义(P>0.05),中药治疗组与模型组比较PT有所缩短,差异不显著,不具有统计学意义(P>0.05),中药治疗组与对照组比较差异不显著,无统计学意义(P>0.05)。
2.2 APTT:模型组APTT轻度延长,与对照组比较差异不显著,不具有统计学意义(P>0.05),中药治疗组与模型组比较APTT有所缩短,差异不显著,不具有统计学意义(P>0.05),中药治疗组与对照组比较差异不显著,无统计学意义(P>0.05)。
2.3 FIB:模型组FIB显著增高,与对照组比较差异显著,具有统计学意义(P<0.01),中药治疗组与模型组比较FIB明显降低,差异显著,具有统计学意义(P<0.01),但仍较对照组增高(P<0.01)。
2.4 INR:模型组INR值轻度增高,与对照组比较差异不显著,不具有统计学意义(P>0.05),中药治疗组与模型组比较INR无明显变化,差异不具有统计学意义(P>0.05),中药治疗组与对照组比较差异不显著,无统计学意义(P>0.05)。
结论:中药茵陈承气汤可有效降低重症急性胰腺炎大鼠血液纤维蛋白原(FIB)含量,改善重症急性胰腺炎大鼠凝血功能障碍严重程度,为临床治疗SAP提供理论依据和新的思路;重症急性胰腺炎大鼠的纤维蛋白原(FIB)在短时间内明显升高,凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、国际标准化比率(INR)在24h内未出现明显变化;中药茵陈承气汤对重症急性胰腺炎大鼠的凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、国际标准化比率(INR)在短时间内无明显影响。
Objective: To explore the effect of Yinchenchengqi Tang on cruor function in severe acute pancreatitis (SAP) in rats. and provide a theoretical basis and new treatment ideas for the treatment of SAP of traditional Chinese medicine.
Methods: Healthy SD 30 male rats were randomly divided into three groups: control group; model group; Chinese medicine treatment group.each group 10. Model-building steps:(1)anesthesia with 4% sodium pentobar bital in accordance with 1 ml / kg body weight by intraperitoneal injection of doses of narcotic. (2)anesthesia after the entry into force, fixed in rats in the surgery board, check supine position, abdominal rat hair cut, conven- tional disinfection Shop surgical towel ready.(3)from upper middle incision about 3 cm in the open under strict aseptic abdominal operation to find the junction of stomach and duodenum, and then down to find the opening cholangiopancreatography duodenum. (4)hepatic artery occlusion Explorer folder, No.4.5 needles will be up on the flat cholangiopancreato graphy subcapsular duodenal puncture opening line to the duct direction about 2-3mm, by 1 ml / kg body weight will be 4% sodium deoxycholate to retro- grade pancreatic duct injection of (0.2 ml / min), so that uniform uplift of the pancreatic duct from the needle, that needle into the hand of oppression of approximately 3-5 min, then remove the folder artery, abdominal closure, and at the same time to observe the beginning of time. control group was sham-operated group, model group by the pancreatic duct to the pancreatic tissue in rats by retrograde injection of 4% sodium deoxycholate method of severe acute pancreatitis in rats induced by severe acute pancreatitis (SAP), Chinese medicine treatment group and model group SAP produced the same rat model, the former model in 12h and 12 h after the model oral gavage Yinchenchengqi Tang liquid 2ml / g, the rats on the prothrombin time (PT) , activated partial thromboplastin time (APTT), fibrinogen (FIB) and international normalized ratio (INR) compared to each other.
Results:
(1)PT: mild extension of PT of the model group, compared with the control group did not have statistically significant differences (P> 0.05), traditional Chinese medicine treatment group compared with the model group has been to shorten the PT, the difference is not statistically significant (P> 0.05), Chinese medicine treatment group compared with the control group there was no significant difference (P> 0.05).
(2)APTT: model group with mild APTT prolonged, compared with the control group did not have statistically significant differences (P> 0.05), traditional Chinese medicine treatment group compared with the model group, APTT has been shortened, the difference is not statistically significant (P> 0.05), there was no significant difference Chinese medicine treatment group compared with the control group (P> 0.05).
(3)FIB: FIB of the model group was significantly higher, compared with the control group with a statistically significant difference (P <0.01), traditional Chinese medicine treatment group compared with the model group FIB decreased significantly (P <0.01), but still the control group increased (P <0.01).
(4)INR: model group INR increased slightly, compared with the control group did not have statistically significant differences (P> 0.05), traditional Chinese medicine treatment group compared with the model group had no significant changes in INR, the difference is not statistically significant (P> 0.05), Chinese medicine treatment group compared with the control group there was no significant difference (P> 0.05).
Conclusion: Yinchenchengqi Tang can effectively reduce severe acute pancreatitis in rat blood fibrinogen (FIB) content, to improve blood coagulation in rats with severe acute pancreatitis severity of dysfunction for clinical treatment of SAP to provide the theoretical basis and new ideas; severe acute pancreatitis in rats fibrinogen (FIB) was significantly increased, prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio (INR) in the 24h does not significantly change; Yinchenchengqi Tang had no significant effect on rats with severe acute pancreatitis on the prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio (INR) in a short period of time.
方法:健康SD雄性大鼠30只,随机分为三组:对照组;模型组;中药治疗组(简称中药组);每组10只。模型建立的步骤:(1)麻醉:用4%的戊巴比妥钠按1ml/kg体重的剂量经腹腔注射麻醉。(2)麻醉生效后,固定大鼠于手术板上,取仰卧位,剪去腹部鼠毛,常规消毒,铺巾准备手术。(3)取上腹正中切口3cm左右,在严格无菌操作下打开腹腔,找到胃十二指肠交界处,再向下寻找到胰胆管十二指肠开口处。(4)用动脉夹夹闭肝总管,将4.5号针头平面朝上于胰胆管十二指肠开口处行被膜下穿刺,并向胰管方向推进约2-3mm,按1ml/kg体重将1%去氧胆酸钠逆行推注至胰管中(0.2ml/min),使整个胰胆管均匀隆起,退出针头,用手压迫进针处约3-5min,然后去除动脉夹,关腹,并同时开始计时观察。对照组为假手术组;模型组采用经胰胆管向大鼠胰腺组织内逆行注射1%去氧胆酸钠的方法诱导大鼠重症急性胰腺炎(SAP),中药组采用与模型组相同的方法制造SAP大鼠模型,并分别于造模前12h和造模后12h经口灌服中药茵陈承气汤水煎药液2ml/g。造模24h后采血,化验血液凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(FIB)及国际标准化比率(INR),对各组大鼠化验结果进行两两比较。
结果:
2.1 PT:模型组PT轻度延长,与对照组比较差异不显著,不具有统计学意义(P>0.05),中药治疗组与模型组比较PT有所缩短,差异不显著,不具有统计学意义(P>0.05),中药治疗组与对照组比较差异不显著,无统计学意义(P>0.05)。
2.2 APTT:模型组APTT轻度延长,与对照组比较差异不显著,不具有统计学意义(P>0.05),中药治疗组与模型组比较APTT有所缩短,差异不显著,不具有统计学意义(P>0.05),中药治疗组与对照组比较差异不显著,无统计学意义(P>0.05)。
2.3 FIB:模型组FIB显著增高,与对照组比较差异显著,具有统计学意义(P<0.01),中药治疗组与模型组比较FIB明显降低,差异显著,具有统计学意义(P<0.01),但仍较对照组增高(P<0.01)。
2.4 INR:模型组INR值轻度增高,与对照组比较差异不显著,不具有统计学意义(P>0.05),中药治疗组与模型组比较INR无明显变化,差异不具有统计学意义(P>0.05),中药治疗组与对照组比较差异不显著,无统计学意义(P>0.05)。
结论:中药茵陈承气汤可有效降低重症急性胰腺炎大鼠血液纤维蛋白原(FIB)含量,改善重症急性胰腺炎大鼠凝血功能障碍严重程度,为临床治疗SAP提供理论依据和新的思路;重症急性胰腺炎大鼠的纤维蛋白原(FIB)在短时间内明显升高,凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、国际标准化比率(INR)在24h内未出现明显变化;中药茵陈承气汤对重症急性胰腺炎大鼠的凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、国际标准化比率(INR)在短时间内无明显影响。
Objective: To explore the effect of Yinchenchengqi Tang on cruor function in severe acute pancreatitis (SAP) in rats. and provide a theoretical basis and new treatment ideas for the treatment of SAP of traditional Chinese medicine.
Methods: Healthy SD 30 male rats were randomly divided into three groups: control group; model group; Chinese medicine treatment group.each group 10. Model-building steps:(1)anesthesia with 4% sodium pentobar bital in accordance with 1 ml / kg body weight by intraperitoneal injection of doses of narcotic. (2)anesthesia after the entry into force, fixed in rats in the surgery board, check supine position, abdominal rat hair cut, conven- tional disinfection Shop surgical towel ready.(3)from upper middle incision about 3 cm in the open under strict aseptic abdominal operation to find the junction of stomach and duodenum, and then down to find the opening cholangiopancreatography duodenum. (4)hepatic artery occlusion Explorer folder, No.4.5 needles will be up on the flat cholangiopancreato graphy subcapsular duodenal puncture opening line to the duct direction about 2-3mm, by 1 ml / kg body weight will be 4% sodium deoxycholate to retro- grade pancreatic duct injection of (0.2 ml / min), so that uniform uplift of the pancreatic duct from the needle, that needle into the hand of oppression of approximately 3-5 min, then remove the folder artery, abdominal closure, and at the same time to observe the beginning of time. control group was sham-operated group, model group by the pancreatic duct to the pancreatic tissue in rats by retrograde injection of 4% sodium deoxycholate method of severe acute pancreatitis in rats induced by severe acute pancreatitis (SAP), Chinese medicine treatment group and model group SAP produced the same rat model, the former model in 12h and 12 h after the model oral gavage Yinchenchengqi Tang liquid 2ml / g, the rats on the prothrombin time (PT) , activated partial thromboplastin time (APTT), fibrinogen (FIB) and international normalized ratio (INR) compared to each other.
Results:
(1)PT: mild extension of PT of the model group, compared with the control group did not have statistically significant differences (P> 0.05), traditional Chinese medicine treatment group compared with the model group has been to shorten the PT, the difference is not statistically significant (P> 0.05), Chinese medicine treatment group compared with the control group there was no significant difference (P> 0.05).
(2)APTT: model group with mild APTT prolonged, compared with the control group did not have statistically significant differences (P> 0.05), traditional Chinese medicine treatment group compared with the model group, APTT has been shortened, the difference is not statistically significant (P> 0.05), there was no significant difference Chinese medicine treatment group compared with the control group (P> 0.05).
(3)FIB: FIB of the model group was significantly higher, compared with the control group with a statistically significant difference (P <0.01), traditional Chinese medicine treatment group compared with the model group FIB decreased significantly (P <0.01), but still the control group increased (P <0.01).
(4)INR: model group INR increased slightly, compared with the control group did not have statistically significant differences (P> 0.05), traditional Chinese medicine treatment group compared with the model group had no significant changes in INR, the difference is not statistically significant (P> 0.05), Chinese medicine treatment group compared with the control group there was no significant difference (P> 0.05).
Conclusion: Yinchenchengqi Tang can effectively reduce severe acute pancreatitis in rat blood fibrinogen (FIB) content, to improve blood coagulation in rats with severe acute pancreatitis severity of dysfunction for clinical treatment of SAP to provide the theoretical basis and new ideas; severe acute pancreatitis in rats fibrinogen (FIB) was significantly increased, prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio (INR) in the 24h does not significantly change; Yinchenchengqi Tang had no significant effect on rats with severe acute pancreatitis on the prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio (INR) in a short period of time.
引文
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