铁钉菜（Ishige okamurae）抗菌和抑藻活性物质的研究
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摘要
本研究以褐藻门的铁钉菜(Ishige okamurae)为实验材料,经由乙醇、乙醚溶剂浸提,得到提取物浸膏,再通过石油醚、氯仿、乙酸乙酯、正丁醇分级萃取,针对不同萃取相选择运用硅胶柱层析、硅胶薄层层析、大孔树脂层析以及Sephadex-LH20凝胶层析等分离手段,分离得到了铁钉菜数个组分。通过生物活性跟踪实验,从中发现了一些具有较好抗生活性的组分,其中组分IS3.1、IS4具有抑藻活性,其对球形棕囊藻的抑制效果最好,72h的EC_(50)分别达到51.5mg/L和59.3mg/L;氯仿相的Rf0.79条带其具有较强的细菌抑制活性,GC-MS分析发现其主要活性成份可能为(2,2-二甲基-3-甲基磺酰氧基丙基)-4-(-2-甲基-3-环己酮)丁酸;此外,铁钉菜正丁醇萃取相的大孔树脂30%-50%乙醇洗脱物对植物致病真菌的菌丝生长显示出较好的抑制活性。
In this study, the Ishige okamurae were extracted by ethanol and ether to getcrude estracts. Crude extracts were extracted in turn with the petroleum ether, chloroform,ethyl acetate, n-butanol to get different extractive fractions. The different extractivefractions were isolation and purification by appropriate way, for example the silica gelcolumn chromatography, thin layer chromatography, macroporous-resin chromatographyand the Sephadex-LH20 gel chromatography. Several components were got from theextractive fractions. These components were tested for their antibiotic activities againstsome fungi, bacteria and algae, eventually some antibiotic components were found. Thecomponent IS3.1 and IS4 showed strong resistance to the growth of alage, which the 72hEC_(50) to globosa respectively up to 51.5mg/L and 59.3mg/L; The Rf0.79 TLC fractionfrom chloroform extract has strong inhibitory activity to bacteria, GC-MS analysisshowed that the main active ingredient of Rf0.79 fraction as possible is(2,2-dimethyl-3-methylsulfonyloxypropyl)-4-(2-methyl-3-oxocyclohexyl)butanoate; The n-butanol extractof Ishige okamurae Yendo was chromatography in macroporous resin and the 30%-50%ethanol eluate showed good inhibitory activity to the growth of some plant pathogenicfungi hypha.
In this study, the Ishige okamurae were extracted by ethanol and ether to getcrude estracts. Crude extracts were extracted in turn with the petroleum ether, chloroform,ethyl acetate, n-butanol to get different extractive fractions. The different extractivefractions were isolation and purification by appropriate way, for example the silica gelcolumn chromatography, thin layer chromatography, macroporous-resin chromatographyand the Sephadex-LH20 gel chromatography. Several components were got from theextractive fractions. These components were tested for their antibiotic activities againstsome fungi, bacteria and algae, eventually some antibiotic components were found. Thecomponent IS3.1 and IS4 showed strong resistance to the growth of alage, which the 72hEC_(50) to globosa respectively up to 51.5mg/L and 59.3mg/L; The Rf0.79 TLC fractionfrom chloroform extract has strong inhibitory activity to bacteria, GC-MS analysisshowed that the main active ingredient of Rf0.79 fraction as possible is(2,2-dimethyl-3-methylsulfonyloxypropyl)-4-(2-methyl-3-oxocyclohexyl)butanoate; The n-butanol extractof Ishige okamurae Yendo was chromatography in macroporous resin and the 30%-50%ethanol eluate showed good inhibitory activity to the growth of some plant pathogenicfungi hypha.
引文
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