峨眉山产中药材HPLC指纹图谱初探
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摘要
中药材HPLC指纹图谱技术是利用高效液相色谱仪,宏观分析中药材复杂的化学特征信息,以整体性和模糊性原则定性定量反映中药材遗传物种差异性和后天个体差异性的技术。它是中药材质量控制的重要内容,对推进中医药现代化发展和普遍适用有积极意义。
本文首先以峨眉山产药材侧柏叶为例,探索色谱分离条件,包括流动相、检测波长、色谱柱、流速和柱温、进样量、梯度洗脱程序,以槲皮素含量为对照成分比较分析不同加工炮制方法对侧柏叶中黄酮类成分的影响,证实中药材质量控制中建立指纹图谱的必要性,又以峨眉山产药材仙鹤草、夏枯草和地锦草为例分析指纹图谱建立过程中色谱条件的优化选择,尤其梯度洗脱程序梯度陡度和宽度选择优化,阐述了结合中药材不同部位化学成分组成和含量进行色谱条件优化的常规过程。
本文初步探索峨眉山产几种中药材HPLC指纹图谱分析方法的初步建立,提出中药材高效液相色谱指纹图谱数据构建的模式,同时作为自然保护区生物标本整理整合及共享项目的子课题研究,为后期峨眉山产药用植物标准色谱指纹图谱建立和分析评价等提供前期研究基础工作。
Traditional Chinese medicinal materials HPLC fingerprint technology is the macro-analysis of the complex chemical characteristics information by use of high-performance liquid chromatography, which reflects the differences in genetic species and individual according to the integrity and fuzzy principles by qualitative and quantitative method. It is an important content in traditional Chinese medicinal materials quality control promoting the modernization and universal application of Chinese medicine development and having positive significance.
Firstly we take Platycladus orientalis(L.)Franco from Emei Mountains as an example to explore separation conditions, including the mobile phase, detection wavelength, column, flow and temperature, the sample volume and the gradient elution procedures, analyzing the quercetin content influence of the different processing methods to emphasize the importance of controlling sources in the establishment of the fingerprint, secondly we take Agrimonia pilosa Ledeb., Prunella vulgaris L. and Euphorbia humifusa Willd. as examples to analyze the conditions for optimization in fingerprint establishment, especially the steep and width choice in gradient elution procedures, illustrating the general process of separation conditions optimization under the guideline of the different parts of traditional Chinese medicinal materials and the different content chemical composition.
This article initially explore the traditional Chinese medicinal materials HPLC fingerprint establishing methods, bringing forward the framework of construction for traditional Chinese medicinal materials HPLC fingerprint data, at the same time as a part of the research projects Biological samples integration and sharing in nature reserve, providing an initial basis and reference data for the latter part of the Emei Mountains traditional Chinese medicinal materials standard chromatographic fingerprint establishment and evaluation.
本文首先以峨眉山产药材侧柏叶为例,探索色谱分离条件,包括流动相、检测波长、色谱柱、流速和柱温、进样量、梯度洗脱程序,以槲皮素含量为对照成分比较分析不同加工炮制方法对侧柏叶中黄酮类成分的影响,证实中药材质量控制中建立指纹图谱的必要性,又以峨眉山产药材仙鹤草、夏枯草和地锦草为例分析指纹图谱建立过程中色谱条件的优化选择,尤其梯度洗脱程序梯度陡度和宽度选择优化,阐述了结合中药材不同部位化学成分组成和含量进行色谱条件优化的常规过程。
本文初步探索峨眉山产几种中药材HPLC指纹图谱分析方法的初步建立,提出中药材高效液相色谱指纹图谱数据构建的模式,同时作为自然保护区生物标本整理整合及共享项目的子课题研究,为后期峨眉山产药用植物标准色谱指纹图谱建立和分析评价等提供前期研究基础工作。
Traditional Chinese medicinal materials HPLC fingerprint technology is the macro-analysis of the complex chemical characteristics information by use of high-performance liquid chromatography, which reflects the differences in genetic species and individual according to the integrity and fuzzy principles by qualitative and quantitative method. It is an important content in traditional Chinese medicinal materials quality control promoting the modernization and universal application of Chinese medicine development and having positive significance.
Firstly we take Platycladus orientalis(L.)Franco from Emei Mountains as an example to explore separation conditions, including the mobile phase, detection wavelength, column, flow and temperature, the sample volume and the gradient elution procedures, analyzing the quercetin content influence of the different processing methods to emphasize the importance of controlling sources in the establishment of the fingerprint, secondly we take Agrimonia pilosa Ledeb., Prunella vulgaris L. and Euphorbia humifusa Willd. as examples to analyze the conditions for optimization in fingerprint establishment, especially the steep and width choice in gradient elution procedures, illustrating the general process of separation conditions optimization under the guideline of the different parts of traditional Chinese medicinal materials and the different content chemical composition.
This article initially explore the traditional Chinese medicinal materials HPLC fingerprint establishing methods, bringing forward the framework of construction for traditional Chinese medicinal materials HPLC fingerprint data, at the same time as a part of the research projects Biological samples integration and sharing in nature reserve, providing an initial basis and reference data for the latter part of the Emei Mountains traditional Chinese medicinal materials standard chromatographic fingerprint establishment and evaluation.
引文
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