摄涕止鼽汤对肾阳虚型变应性鼻炎大鼠血清细胞因子IL-5、ECP的影响
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摘要
目的:本课题旨在以肾阳虚型变应性鼻炎动物模型为研究对象,从病理、炎症介质的角度来探索摄涕止鼽汤对肾阳虚型变应性鼻炎的治疗作用,从而揭示出摄涕止鼽汤治疗肾阳虚型变应性鼻炎的作用机制,为今后临床用药提供理论依据。
方法:本研究采用糖皮质激素氢化可的松注射液及恐吓法复制肾阳虚模型,在此基础上再用卵清蛋白致敏复制变应性鼻炎大鼠模型。先将大鼠随机分为空白对照组10只、模型组50只,模型组进行造模,共35天;经评价模型复制成功以后,再把模型组随机分为肾阳虚型变应性鼻炎组10只,摄涕止鼽汤高、中、低剂量组各10只、西药酮替芬组10只。造模结束后,治疗各组每日给予不同的药物1ml/100g,共7天,空白对照组与肾阳虚变应性鼻炎模型组给以同样量的生理盐水。除空白对照组外,其他各组在给药结束后1小时再予以2%卵蛋白溶液激发。造模第一天开始,观察动物一般情况变化,并在滴入卵蛋白溶液30分钟内,着重观察鼻部症状。给药完毕后,处死动物。每组随机选取6只大鼠,剥离上颌骨摘取鼻中隔,观察在光镜下的病理形态变化,并且取大鼠血清通过ELISA法检测白细胞介素-5(IL-5)、嗜酸性细胞阳离子蛋白(ECP)的含量。
结果:摄涕止鼽汤高剂量组能明显缓解大鼠AR症状,改善大鼠肾阳虚症状,能明显降低大鼠鼻中隔粘膜嗜酸性粒细胞(EOS)浸润的病理状态,还能显著降低病态中大鼠血清ECP、IL-5的含量,与阳性对照西药酮替芬组疗效相似,无显著性差异(p＞0.05)。
结论:本研究证明摄涕止鼽汤治疗肾阳虚型变应性鼻炎确有疗效,它不仅能缓解动物喷嚏、挠鼻、流涕等AR症状;还能缓解动物体重增长缓慢、饮食减少、精神萎靡、畏寒肢冷、小便量多、蜷缩懒动、喜扎堆等类似肾阳虚症状;还能改善鼻中隔粘膜嗜酸性粒细胞(EOS)增多的病理状态;而且还可以降低病态中血清ECP、IL-5的含量,从而得出摄涕止鼽汤治疗肾阳虚型变应性鼻炎的机制之一是通过降低IL-5的含量,从而降低EOS在鼻粘膜浸润及循环血液中分化、成熟、增殖、活化,最终降低了ECP的细胞毒性作用。
OBJECTIVE:In this topic,we study in order to establish the complex model of Allergic rhinitis(AR) in deficiency of kidney-YANG type,and reveal the effect of the traditional Chinese medicine of SheTiZhiQiu-decoction to Allergic rhinitis(AR) in deficiency of kidney-YANG type in this disease from the aspects of patho-shapes、mediators of inflammation and so on.We hope to provide some theory base in the clinical medication.
METHODS:In this topic,the model of deficiency of kidney-YANG type is established with Hydrocortisone Injection and Threaten.The model of AR is established with ovalbumin.6 groups are involved which are the normal group,the model group,the SheTiZhiQiu-decoction group(the high-dose group,the middle-dose group,the low-dose group),the Ketoti-fen group.There are 10 rats in every group. Other groups are established into models except the normal group during 34 days.After the model is established,1ml different medicine are given to the groups except the normal group in 10 days.During the treatment phase,the rats are stimulating by 2%OVA 1 hour after the medicine administrate.From the first day,we observe the common symptoms especially nasal symptoms in 30 minutes with OVA stimulating. After treatment,rats are killed.We choice random 6 rats in every group to take their nasal septums,then observe the pathogenic changes happened with electron microscope.Meanwhile,with elisa IL-5、ECP are detected in their blood serum.
RESULTS:The high-dose group SheTiZhiQiu-decoction can release rat's symptom of AR and the deficiency of kidney-YANG,also can decrease EOS' amount in the tunica mucosa of nasal septum and decrease the content of ECP and IL-5.The efficacy of the high-dose group of SheTiZhiQiu-decoction is best.It is the same as the group of Ketotifen-pills.There is no great different between two groups.
CONCLUSION:SheTiZhiQiu-decoction's effect is testified in the complex model.Not only it can release rat's sneese、scratching nose、nasal discharge.Also release rat's body weight、decrease drinking、keep depressed、keep chilly、increase urination、keep scrunch、like crowding.Also decrease EOS' amount in the tunica mucosa of nasal septum and decrease the content of ECP and IL-5.In a word,SheTiZhiQiu-decoction can decrease EOS' infiltrating in the tunica mucosa of nasal septum and the degree of differentiation、maturation、proliferation、activation,also decrease the cytotoxicity of ECP.They may be the mechanism of therapeutic action to rats of AR in deficiency of kidney-YANG types.
方法:本研究采用糖皮质激素氢化可的松注射液及恐吓法复制肾阳虚模型,在此基础上再用卵清蛋白致敏复制变应性鼻炎大鼠模型。先将大鼠随机分为空白对照组10只、模型组50只,模型组进行造模,共35天;经评价模型复制成功以后,再把模型组随机分为肾阳虚型变应性鼻炎组10只,摄涕止鼽汤高、中、低剂量组各10只、西药酮替芬组10只。造模结束后,治疗各组每日给予不同的药物1ml/100g,共7天,空白对照组与肾阳虚变应性鼻炎模型组给以同样量的生理盐水。除空白对照组外,其他各组在给药结束后1小时再予以2%卵蛋白溶液激发。造模第一天开始,观察动物一般情况变化,并在滴入卵蛋白溶液30分钟内,着重观察鼻部症状。给药完毕后,处死动物。每组随机选取6只大鼠,剥离上颌骨摘取鼻中隔,观察在光镜下的病理形态变化,并且取大鼠血清通过ELISA法检测白细胞介素-5(IL-5)、嗜酸性细胞阳离子蛋白(ECP)的含量。
结果:摄涕止鼽汤高剂量组能明显缓解大鼠AR症状,改善大鼠肾阳虚症状,能明显降低大鼠鼻中隔粘膜嗜酸性粒细胞(EOS)浸润的病理状态,还能显著降低病态中大鼠血清ECP、IL-5的含量,与阳性对照西药酮替芬组疗效相似,无显著性差异(p＞0.05)。
结论:本研究证明摄涕止鼽汤治疗肾阳虚型变应性鼻炎确有疗效,它不仅能缓解动物喷嚏、挠鼻、流涕等AR症状;还能缓解动物体重增长缓慢、饮食减少、精神萎靡、畏寒肢冷、小便量多、蜷缩懒动、喜扎堆等类似肾阳虚症状;还能改善鼻中隔粘膜嗜酸性粒细胞(EOS)增多的病理状态;而且还可以降低病态中血清ECP、IL-5的含量,从而得出摄涕止鼽汤治疗肾阳虚型变应性鼻炎的机制之一是通过降低IL-5的含量,从而降低EOS在鼻粘膜浸润及循环血液中分化、成熟、增殖、活化,最终降低了ECP的细胞毒性作用。
OBJECTIVE:In this topic,we study in order to establish the complex model of Allergic rhinitis(AR) in deficiency of kidney-YANG type,and reveal the effect of the traditional Chinese medicine of SheTiZhiQiu-decoction to Allergic rhinitis(AR) in deficiency of kidney-YANG type in this disease from the aspects of patho-shapes、mediators of inflammation and so on.We hope to provide some theory base in the clinical medication.
METHODS:In this topic,the model of deficiency of kidney-YANG type is established with Hydrocortisone Injection and Threaten.The model of AR is established with ovalbumin.6 groups are involved which are the normal group,the model group,the SheTiZhiQiu-decoction group(the high-dose group,the middle-dose group,the low-dose group),the Ketoti-fen group.There are 10 rats in every group. Other groups are established into models except the normal group during 34 days.After the model is established,1ml different medicine are given to the groups except the normal group in 10 days.During the treatment phase,the rats are stimulating by 2%OVA 1 hour after the medicine administrate.From the first day,we observe the common symptoms especially nasal symptoms in 30 minutes with OVA stimulating. After treatment,rats are killed.We choice random 6 rats in every group to take their nasal septums,then observe the pathogenic changes happened with electron microscope.Meanwhile,with elisa IL-5、ECP are detected in their blood serum.
RESULTS:The high-dose group SheTiZhiQiu-decoction can release rat's symptom of AR and the deficiency of kidney-YANG,also can decrease EOS' amount in the tunica mucosa of nasal septum and decrease the content of ECP and IL-5.The efficacy of the high-dose group of SheTiZhiQiu-decoction is best.It is the same as the group of Ketotifen-pills.There is no great different between two groups.
CONCLUSION:SheTiZhiQiu-decoction's effect is testified in the complex model.Not only it can release rat's sneese、scratching nose、nasal discharge.Also release rat's body weight、decrease drinking、keep depressed、keep chilly、increase urination、keep scrunch、like crowding.Also decrease EOS' amount in the tunica mucosa of nasal septum and decrease the content of ECP and IL-5.In a word,SheTiZhiQiu-decoction can decrease EOS' infiltrating in the tunica mucosa of nasal septum and the degree of differentiation、maturation、proliferation、activation,also decrease the cytotoxicity of ECP.They may be the mechanism of therapeutic action to rats of AR in deficiency of kidney-YANG types.
引文
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