降解HNS生产废水的复合型微生物絮凝剂产生菌筛选及应用研究
|
摘要
本论文从六硝基茋生产废水、被六硝基茋生产废水污染的土壤和杨树枝上,经过分离筛选驯化,得到几种微生物絮凝剂产生菌,经复合最后筛选出一种较高效的复合型微生物絮凝剂产生菌;通过单因素实验确定其最佳的培养条件和絮凝条件,将其应用到六硝基茋生产废水的处理中,并进行絮凝活性分布的测定,对所产复合型微生物絮凝剂进行了分离纯化及初步鉴定,实验结果表明:
共得到15种纯种菌落从六硝基茋生产废水及被其污染的土壤、杨树枝中用六种富集培养基经多次分离、纯化得到,经驯化和对其絮凝率的测定,获得7株耐六硝基茋废水的具有絮凝活性的高效菌种,经驯化它们的絮凝率均发生了变化,有增有减,这说明驯化对微生物絮凝剂产生菌的产絮能力有一定的影响,选择5株具有较高絮凝活性的菌种进行复合,得到絮凝率大大高于单一菌群的复合菌群MBF_4,其絮凝率达77.60%。
复合型微生物絮凝剂产生菌MBF_4的培养基经优化其最佳碳源为复杂糖淀粉,最佳氮源为蛋白胨,絮凝剂产生的最适宜pH值为5。
复合型微生物絮凝剂产生菌MBF_4对高岭土悬浊液的絮凝条件经优化,其最佳絮凝条件为:投加量3mL,pH值8,金属离子为Ca~(2+)。
复合型微生物絮凝剂产生菌MBF_4在处理六硝基菧生产废水时最佳投加量为6ml,最佳pH值为8,对六硝基茋生产废水色度、COD的去除率可达93.33%、73.08%,为六硝基茋生产废水的后续处理提供了有利条件。
复合型微生物絮凝剂产生菌MBF_4的絮凝活性分布在上清液中,而不是菌体细胞自身,通过分离提纯,最终得到微生物絮凝剂,经鉴定其不仅含有蛋白质类物质,而且还含有糖类物质。
The study firstly screened a single strain capable of HNS wastewater-degrading which can produce MBF from HNS wastewater,the contaminated soil and poplar tree branches to be HNS wastewater, the complex bioflocculant with higher flocculation activity than MBF produced by a single strain were constructed. The optimize culture conditions and flocculation condition of the selected strain were ascertained through single factor experiment. The application of the complex bioflocculant in HNS wastewater treatment was examined in the paper. In the end,Flocculation active distribution was determined and chemical structural characteristics have been researched.
15 strain were screened from HNS wastewater,the contaminated soil and poplar tree branches to be HNS wastewater, and the 7 strain with flocculant activity are screened. Those strain’s flocculation activity change in the process of domesticated. This phenomenon indicates that domesticated have an impact on the flocculant production. The complex bioflocculant with higher flocculation activity than MBF produced by a single strain were constructed, the flocculation could reach 77.60%.
The studies show that carbon source for this strain to producing flocculant is soluble starch , optimum nitrogen source is peptone, optimal initial pH is 5.
The studies show that the optimum flocculation condition is that the flocculants' dosage is 3ml, pH value is 8, metal ion is Ca~(2+).
The studies show that the optimum flocculation condition is that the flocculants' dosage is 6ml, pH value is 8 in the HNS wastewater treatment. The ratio of removing chromaticity and COD of HNS wastewater-degrading is respectively up to 93.333percent and 73.08 percent.
The complex bioflocculant are excreted out of the cell,and the flocculants are mostly distributed in the supernatant. The complex bioflocculant is not only sugar but also protein.
共得到15种纯种菌落从六硝基茋生产废水及被其污染的土壤、杨树枝中用六种富集培养基经多次分离、纯化得到,经驯化和对其絮凝率的测定,获得7株耐六硝基茋废水的具有絮凝活性的高效菌种,经驯化它们的絮凝率均发生了变化,有增有减,这说明驯化对微生物絮凝剂产生菌的产絮能力有一定的影响,选择5株具有较高絮凝活性的菌种进行复合,得到絮凝率大大高于单一菌群的复合菌群MBF_4,其絮凝率达77.60%。
复合型微生物絮凝剂产生菌MBF_4的培养基经优化其最佳碳源为复杂糖淀粉,最佳氮源为蛋白胨,絮凝剂产生的最适宜pH值为5。
复合型微生物絮凝剂产生菌MBF_4对高岭土悬浊液的絮凝条件经优化,其最佳絮凝条件为:投加量3mL,pH值8,金属离子为Ca~(2+)。
复合型微生物絮凝剂产生菌MBF_4在处理六硝基菧生产废水时最佳投加量为6ml,最佳pH值为8,对六硝基茋生产废水色度、COD的去除率可达93.33%、73.08%,为六硝基茋生产废水的后续处理提供了有利条件。
复合型微生物絮凝剂产生菌MBF_4的絮凝活性分布在上清液中,而不是菌体细胞自身,通过分离提纯,最终得到微生物絮凝剂,经鉴定其不仅含有蛋白质类物质,而且还含有糖类物质。
The study firstly screened a single strain capable of HNS wastewater-degrading which can produce MBF from HNS wastewater,the contaminated soil and poplar tree branches to be HNS wastewater, the complex bioflocculant with higher flocculation activity than MBF produced by a single strain were constructed. The optimize culture conditions and flocculation condition of the selected strain were ascertained through single factor experiment. The application of the complex bioflocculant in HNS wastewater treatment was examined in the paper. In the end,Flocculation active distribution was determined and chemical structural characteristics have been researched.
15 strain were screened from HNS wastewater,the contaminated soil and poplar tree branches to be HNS wastewater, and the 7 strain with flocculant activity are screened. Those strain’s flocculation activity change in the process of domesticated. This phenomenon indicates that domesticated have an impact on the flocculant production. The complex bioflocculant with higher flocculation activity than MBF produced by a single strain were constructed, the flocculation could reach 77.60%.
The studies show that carbon source for this strain to producing flocculant is soluble starch , optimum nitrogen source is peptone, optimal initial pH is 5.
The studies show that the optimum flocculation condition is that the flocculants' dosage is 3ml, pH value is 8, metal ion is Ca~(2+).
The studies show that the optimum flocculation condition is that the flocculants' dosage is 6ml, pH value is 8 in the HNS wastewater treatment. The ratio of removing chromaticity and COD of HNS wastewater-degrading is respectively up to 93.333percent and 73.08 percent.
The complex bioflocculant are excreted out of the cell,and the flocculants are mostly distributed in the supernatant. The complex bioflocculant is not only sugar but also protein.
引文
[1]伦宁,王信东,李玉江.两性聚丙烯酰胺在污泥脱水中的应用[J].山东建材学院学报,1999,13(2):114-116
[2]李玉江,吴涛.高稳定活性硅酸SPSA在污泥脱水应用中的研究[J].环境科学研究,1995,8(6):32-36
[3]张跃军,顾学芳,陈伟忠.阳离子絮凝剂PDA合成与应用研究[J].南京理工大学学报,2001,25(2):205-209 [ 4 ]夏畅斌,黄念东,何湘柱.用聚硅酸铁铝混凝剂处理炼油废水[J].化工环保2000,20(4):29-31
[5] Wang Zhen,Wang Kongxing.Studies on bioflocculant producing microorganisms[J].Aeta blicrobiologiea Sinica,1995,35(2):121-129
[6] Salehizadeh H,Vossoughi M,Alemzadeh I.Some investigations on bioflocculant-producing bacteria[J].Biochem Eng J,2000,5(1):39-44
[7] Shih I L,Van Y T,Yeh L C,et al.Production of a biopolymer flocculant from Bacillus licheniformis and its glocculation properties[J].Bioresource Technology,2001,78(3):267-272
[8]甘光奉,甘莉.高分子絮凝剂研究的进展[J].工业水处理:1999,l9(2):6-7
[9]陈朝东,张志强,赵谊颂.工业水处理技术问答[M].北京:化学工业出版社,2007:263.
[10]周礼,张永奎,陈晓等一种高效微生物絮凝剂产生菌的筛选及培养基优化[J].环境科学学报,2006,26(4):554-555
[11]马放,刘俊良,李淑更等.复合型微生物絮凝剂的开发[J].中国给水排水,2003,19(4):1-4
[12]王建华,刘玉存,刘登程等. HNS的临界厚度测试研究[J].含能材料, 2008,16(2): 146.
[13]宁培毅.六硝基芪的制造与应用[J].现代兵器,1981,09:47-50.
[14]惠君明.六硝基芪炸药及其应用[J].爆破器材,1990,57:9-11.
[15]甘莉,孟召平.微生物絮凝剂的研究进展[J].水处理技术,2006,32(4):5-9
[16] Nakamura J,Miyash Iro S,Hirose Y. Sereening isolation and some properties of microbial cell flocculants[J].Agric Bio Chem,1976,40(20):377-383
[ 17 ] Takagi H,Kadowaki K. Flocculant production [J]. Agric. Boil. Chem.,1985,49(11):3151-3157
[18] Kurane R.,Toeda K.,Takeda K.,et al.Screening for and charaeteristics of mircrobial flocculation[J].Agrie.Boil.Chem.,1986,50(9):2309-231 [ 19 ] Kurane R,MatsuyamaH. Production of a bioflocculant by mixed culture[J] Biotechnol,Biochem.1994,(58):253-257
[20] Suh H-H,et al. Characterization of bioflocculant produced by Bacillus sp. P15[J].Journal of Fermentation and Bioengineering,1997,184(2):108-112.
[21] Watanabe M , et al. Flocculating p roperty of extracellular polymeric substance derived form a marine photosynthtic bacterium, rhodovulum sp[J]. Journal of Bioscience and Bioengineering, 1999, (5) : 45-48.
[22] Shih I.L,Van Y T,et al.Prodction of a biopolymer flocculant from Bacillus Lichenfformis and its flocculation properties[J].bio-umc Technology,2001,(78):267-272. [ 23 ] H.Salehizadeh,S.A. Shojaosadat ilsolation and characterization of a bioflocclant produced by Bacillus firmus[J].Bioechnology Letters,2002,(24):35-40.
[24]马放,刘俊良,李淑更等.复合型微生物絮凝剂的开发[J].中国给排水,2003,l(19):4-7
[25]游映玖.Y5复合产絮菌及其产絮物絮凝特性试验研究[J].环境科学与技术, 2003,26(l):4-6
[26]王琴,马放,孟路等.补加葡萄糖对复合型生物絮凝剂生产的影响[J].给水排水,2004,30(10):52-54
[27]董双石,王爱杰,任南琪等.提高复合型产絮菌F2-F6产絮能力的驯化方法.生物加工过程[J],2004,2(3):37-39
[28]靳慧霞,马放,盂路.复合型微生物絮凝剂与化学絮凝剂的复配及其应用[J].化工进展,2006,25(l):105-109
[29]关毅,张娟.EM复合菌群产絮凝剂的絮凝性能[J].水处理技术,2006,23(7) :42-45
[30]张玉玲,张兰英,任何军等.高产絮凝剂复合菌的基因分型及絮凝效果研究[J].中国给水排水,2006,22(11):35-44
[31]马会强,张兰英,张玉玲等.高压对复合型微生物絮凝剂产生菌的性质和絮凝活性的影响[J].吉林大学学报(理学版),2006,44(6):1023-1027
[32]杜宝山,张玉玲,张兰英等.复合型微生物絮凝剂产生菌生长周期中参数变化[J].吉林大学学报(理学报),2007,45(l):138-142
[33]张志强,林波,夏四清等.复合菌群的构建及其所产微生物絮凝剂的动力学研究[J].环境工程学报,2007,l(l):74-77 [ 34 ]郭琇 ,孙洪伟.新型复合型生物絮凝剂的制备与应用研究[J].生物技术,2010, 20(5)83-87 [ 35 ]江锋,黄晓武,胡勇有.胞外生物高聚物絮凝剂的研究进展(下)[J].给水排水,2002,28(9):88-91. [ 36 ]江锋,黄晓武,胡勇有.胞外生物高聚物絮凝剂的研究进展(上)[J].给水排水,2002,28(8):83-89.
[37]周群英,高廷耀.环境工程微生物学[M].北京:高等教育出版社, 2000: 25-30.
[38]陈坚,任洪强等.环境生物技术应用与发展[M].北京:中国轻工业出版社, 2001: 15-38.
[39] Lee SH, Lee SO, Jang K L. Microbial flocculant from Arcuadendrom sp.TS-49[J]. Biotechnology Letters, 1995,17(1): 95-100.
[40]马放,杨基先等.环境生物制剂的开发与应用[M].北京:化学工业出版社,2004: 17-45.
[41]周少奇.环境生物技术[M].北京:科学出版社, 2003: 130-158.
[42]王兰, .张清敏等现代环境微生物学[M].北京:化学工业出版社, 2006: 240-242.
[43]刘紫鹃,刘志培等.一株产微生物絮凝剂菌株的分离鉴定及特性[J].微生物学通报, 2001,28(1): 5-8.
[44]唐受印,戴友芝等.废水处理工程[M].北京:化学工业出版社, 2004: 27.
[45]刘秉涛.壳聚糖复合剂在水处理中的净化效能研究.工学博士学位论文.黑龙江哈尔滨:哈尔滨工业大学,2007.
[46]郭嘉昒.微生物絮凝剂产生菌筛选及絮凝特性研究.工学硕士学位论文.山西省太原市:中北大学,2010.
[47]张兰英,刘娜,孙立波等.现代环境微生物技术[M].北京:.清华大学出版社,2005:354.
[48]李素玉.环境微生物分类与检测技术[M].北京:化学工业出版社,2005:219.
[49]尹华等.微生物絮凝剂的研究现状与发展趋势[J],云南环境科学,2000,19:22-29
[50]刘彬彬.适用于处理造纸中段废水的微生物絮凝剂的制备及应用研究.工学硕士学位论文.江苏省镇江市:江苏大学,2007.
[51]葛秀村,韩朋利等.生物工程专业教学指导[M].山东:山东大学出版社,2007:562
[52]李唯,宁平主等.云南环境研究循环经济与环境保护[M].云南:云南科技出版社,2006:129.
[53]张致平等.微生物药物学[M].北京:化学工业出版社,2003:595
[54]郑怀礼等.生物絮凝剂与絮凝技术. [M].北京:化学工业出版社,2004:147.
[55]汪多仁.绿色净水处理剂[M].北京:科学技术文献出版社,2006:133
[56]周云,何义亮等.微污染水源净水技术及工程实例[M].北京.化学工业出版社,2003:49
[57]肖锦,周勤等.天然高分子絮凝剂[M].北京.化学工业出版社,2005:392.
[58]杨开,康健雄,张永波等.微生物絮凝剂用于污水强化一级处理[J].中国给水排水,2002,18(2):43-45. [ 59 ]贾省芬,杨彦希,黄志勇等.处理城市污水的多功能混合菌研究[J].环境科学,2000,21(3):81-84.
[60]张志强,林渡.利用啤酒废水所产微生物絮凝剂处理靛蓝印染废水的研究[J].环境污染与防治,2006,28(2):149-151
[61]杨劲峰,赵继红,曲凤华等.微生物絮凝剂用于染料废水脱色及其动力学研究[J].化工科技,2009:17(3):16-19
[62]况金蓉,龚文琪.微生物絮凝剂在石化废水处理中的应用[J].武汉理工大学学报,2002,24(8):38-40
[63]尹华,李桂娇,彭辉等.絮凝剂高产菌的研究及其在水处理中的应用[J].水处理技术,2003,29(5):272-275.
[64]柴晓利,赵有才.微生物絮凝剂用于垃圾填埋场渗滤液后处理的研究[J].黑龙江科技学院学报,2003,13(3):6-9
[65]于皓.垃圾填埋场渗滤液生物絮凝预处理研究[J].能源与境,2007,6 ,87-88
[66]王劲松,胡勇有.微生物絮凝剂促进厌氧污泥颗粒化及其机制的研究[J].环境科学学报,2005,25(3):361-366 [ 67 ]黄民生,史宇凯.微生物絮凝剂净化废水试验研究[J].上海环境科学,2000,19(5):222-224,232.
[68]杨圣云,林波,李昌花,等.微生物絮凝剂HXCS-1处理乳化液废水[J].江西科学,2006,24(2):147-149
[69]李琳,张清敏,杨建华.复合微生物絮凝处理红薯淀粉废水的研究[J].环境科学与技术,2006,29(7):75-76
[70]叶明主.微生物学实验技术[M].安徽省合肥:合肥工业大学出版社,2009:36
[71]李素清.胞外生物絮凝剂的制备与特性研究.工学硕士学位论文.湖南:湖南大学,2008
[72] COD测定仪使用说明书
[73]蒋立科,罗曼.生物化学实验设计与实践[M].合肥:高等教育出版社,2006:196-240.
[74]丛峰松.生物化学实验[M].上海:上海交通大学出版社,2005:83-84
[2]李玉江,吴涛.高稳定活性硅酸SPSA在污泥脱水应用中的研究[J].环境科学研究,1995,8(6):32-36
[3]张跃军,顾学芳,陈伟忠.阳离子絮凝剂PDA合成与应用研究[J].南京理工大学学报,2001,25(2):205-209 [ 4 ]夏畅斌,黄念东,何湘柱.用聚硅酸铁铝混凝剂处理炼油废水[J].化工环保2000,20(4):29-31
[5] Wang Zhen,Wang Kongxing.Studies on bioflocculant producing microorganisms[J].Aeta blicrobiologiea Sinica,1995,35(2):121-129
[6] Salehizadeh H,Vossoughi M,Alemzadeh I.Some investigations on bioflocculant-producing bacteria[J].Biochem Eng J,2000,5(1):39-44
[7] Shih I L,Van Y T,Yeh L C,et al.Production of a biopolymer flocculant from Bacillus licheniformis and its glocculation properties[J].Bioresource Technology,2001,78(3):267-272
[8]甘光奉,甘莉.高分子絮凝剂研究的进展[J].工业水处理:1999,l9(2):6-7
[9]陈朝东,张志强,赵谊颂.工业水处理技术问答[M].北京:化学工业出版社,2007:263.
[10]周礼,张永奎,陈晓等一种高效微生物絮凝剂产生菌的筛选及培养基优化[J].环境科学学报,2006,26(4):554-555
[11]马放,刘俊良,李淑更等.复合型微生物絮凝剂的开发[J].中国给水排水,2003,19(4):1-4
[12]王建华,刘玉存,刘登程等. HNS的临界厚度测试研究[J].含能材料, 2008,16(2): 146.
[13]宁培毅.六硝基芪的制造与应用[J].现代兵器,1981,09:47-50.
[14]惠君明.六硝基芪炸药及其应用[J].爆破器材,1990,57:9-11.
[15]甘莉,孟召平.微生物絮凝剂的研究进展[J].水处理技术,2006,32(4):5-9
[16] Nakamura J,Miyash Iro S,Hirose Y. Sereening isolation and some properties of microbial cell flocculants[J].Agric Bio Chem,1976,40(20):377-383
[ 17 ] Takagi H,Kadowaki K. Flocculant production [J]. Agric. Boil. Chem.,1985,49(11):3151-3157
[18] Kurane R.,Toeda K.,Takeda K.,et al.Screening for and charaeteristics of mircrobial flocculation[J].Agrie.Boil.Chem.,1986,50(9):2309-231 [ 19 ] Kurane R,MatsuyamaH. Production of a bioflocculant by mixed culture[J] Biotechnol,Biochem.1994,(58):253-257
[20] Suh H-H,et al. Characterization of bioflocculant produced by Bacillus sp. P15[J].Journal of Fermentation and Bioengineering,1997,184(2):108-112.
[21] Watanabe M , et al. Flocculating p roperty of extracellular polymeric substance derived form a marine photosynthtic bacterium, rhodovulum sp[J]. Journal of Bioscience and Bioengineering, 1999, (5) : 45-48.
[22] Shih I.L,Van Y T,et al.Prodction of a biopolymer flocculant from Bacillus Lichenfformis and its flocculation properties[J].bio-umc Technology,2001,(78):267-272. [ 23 ] H.Salehizadeh,S.A. Shojaosadat ilsolation and characterization of a bioflocclant produced by Bacillus firmus[J].Bioechnology Letters,2002,(24):35-40.
[24]马放,刘俊良,李淑更等.复合型微生物絮凝剂的开发[J].中国给排水,2003,l(19):4-7
[25]游映玖.Y5复合产絮菌及其产絮物絮凝特性试验研究[J].环境科学与技术, 2003,26(l):4-6
[26]王琴,马放,孟路等.补加葡萄糖对复合型生物絮凝剂生产的影响[J].给水排水,2004,30(10):52-54
[27]董双石,王爱杰,任南琪等.提高复合型产絮菌F2-F6产絮能力的驯化方法.生物加工过程[J],2004,2(3):37-39
[28]靳慧霞,马放,盂路.复合型微生物絮凝剂与化学絮凝剂的复配及其应用[J].化工进展,2006,25(l):105-109
[29]关毅,张娟.EM复合菌群产絮凝剂的絮凝性能[J].水处理技术,2006,23(7) :42-45
[30]张玉玲,张兰英,任何军等.高产絮凝剂复合菌的基因分型及絮凝效果研究[J].中国给水排水,2006,22(11):35-44
[31]马会强,张兰英,张玉玲等.高压对复合型微生物絮凝剂产生菌的性质和絮凝活性的影响[J].吉林大学学报(理学版),2006,44(6):1023-1027
[32]杜宝山,张玉玲,张兰英等.复合型微生物絮凝剂产生菌生长周期中参数变化[J].吉林大学学报(理学报),2007,45(l):138-142
[33]张志强,林波,夏四清等.复合菌群的构建及其所产微生物絮凝剂的动力学研究[J].环境工程学报,2007,l(l):74-77 [ 34 ]郭琇 ,孙洪伟.新型复合型生物絮凝剂的制备与应用研究[J].生物技术,2010, 20(5)83-87 [ 35 ]江锋,黄晓武,胡勇有.胞外生物高聚物絮凝剂的研究进展(下)[J].给水排水,2002,28(9):88-91. [ 36 ]江锋,黄晓武,胡勇有.胞外生物高聚物絮凝剂的研究进展(上)[J].给水排水,2002,28(8):83-89.
[37]周群英,高廷耀.环境工程微生物学[M].北京:高等教育出版社, 2000: 25-30.
[38]陈坚,任洪强等.环境生物技术应用与发展[M].北京:中国轻工业出版社, 2001: 15-38.
[39] Lee SH, Lee SO, Jang K L. Microbial flocculant from Arcuadendrom sp.TS-49[J]. Biotechnology Letters, 1995,17(1): 95-100.
[40]马放,杨基先等.环境生物制剂的开发与应用[M].北京:化学工业出版社,2004: 17-45.
[41]周少奇.环境生物技术[M].北京:科学出版社, 2003: 130-158.
[42]王兰, .张清敏等现代环境微生物学[M].北京:化学工业出版社, 2006: 240-242.
[43]刘紫鹃,刘志培等.一株产微生物絮凝剂菌株的分离鉴定及特性[J].微生物学通报, 2001,28(1): 5-8.
[44]唐受印,戴友芝等.废水处理工程[M].北京:化学工业出版社, 2004: 27.
[45]刘秉涛.壳聚糖复合剂在水处理中的净化效能研究.工学博士学位论文.黑龙江哈尔滨:哈尔滨工业大学,2007.
[46]郭嘉昒.微生物絮凝剂产生菌筛选及絮凝特性研究.工学硕士学位论文.山西省太原市:中北大学,2010.
[47]张兰英,刘娜,孙立波等.现代环境微生物技术[M].北京:.清华大学出版社,2005:354.
[48]李素玉.环境微生物分类与检测技术[M].北京:化学工业出版社,2005:219.
[49]尹华等.微生物絮凝剂的研究现状与发展趋势[J],云南环境科学,2000,19:22-29
[50]刘彬彬.适用于处理造纸中段废水的微生物絮凝剂的制备及应用研究.工学硕士学位论文.江苏省镇江市:江苏大学,2007.
[51]葛秀村,韩朋利等.生物工程专业教学指导[M].山东:山东大学出版社,2007:562
[52]李唯,宁平主等.云南环境研究循环经济与环境保护[M].云南:云南科技出版社,2006:129.
[53]张致平等.微生物药物学[M].北京:化学工业出版社,2003:595
[54]郑怀礼等.生物絮凝剂与絮凝技术. [M].北京:化学工业出版社,2004:147.
[55]汪多仁.绿色净水处理剂[M].北京:科学技术文献出版社,2006:133
[56]周云,何义亮等.微污染水源净水技术及工程实例[M].北京.化学工业出版社,2003:49
[57]肖锦,周勤等.天然高分子絮凝剂[M].北京.化学工业出版社,2005:392.
[58]杨开,康健雄,张永波等.微生物絮凝剂用于污水强化一级处理[J].中国给水排水,2002,18(2):43-45. [ 59 ]贾省芬,杨彦希,黄志勇等.处理城市污水的多功能混合菌研究[J].环境科学,2000,21(3):81-84.
[60]张志强,林渡.利用啤酒废水所产微生物絮凝剂处理靛蓝印染废水的研究[J].环境污染与防治,2006,28(2):149-151
[61]杨劲峰,赵继红,曲凤华等.微生物絮凝剂用于染料废水脱色及其动力学研究[J].化工科技,2009:17(3):16-19
[62]况金蓉,龚文琪.微生物絮凝剂在石化废水处理中的应用[J].武汉理工大学学报,2002,24(8):38-40
[63]尹华,李桂娇,彭辉等.絮凝剂高产菌的研究及其在水处理中的应用[J].水处理技术,2003,29(5):272-275.
[64]柴晓利,赵有才.微生物絮凝剂用于垃圾填埋场渗滤液后处理的研究[J].黑龙江科技学院学报,2003,13(3):6-9
[65]于皓.垃圾填埋场渗滤液生物絮凝预处理研究[J].能源与境,2007,6 ,87-88
[66]王劲松,胡勇有.微生物絮凝剂促进厌氧污泥颗粒化及其机制的研究[J].环境科学学报,2005,25(3):361-366 [ 67 ]黄民生,史宇凯.微生物絮凝剂净化废水试验研究[J].上海环境科学,2000,19(5):222-224,232.
[68]杨圣云,林波,李昌花,等.微生物絮凝剂HXCS-1处理乳化液废水[J].江西科学,2006,24(2):147-149
[69]李琳,张清敏,杨建华.复合微生物絮凝处理红薯淀粉废水的研究[J].环境科学与技术,2006,29(7):75-76
[70]叶明主.微生物学实验技术[M].安徽省合肥:合肥工业大学出版社,2009:36
[71]李素清.胞外生物絮凝剂的制备与特性研究.工学硕士学位论文.湖南:湖南大学,2008
[72] COD测定仪使用说明书
[73]蒋立科,罗曼.生物化学实验设计与实践[M].合肥:高等教育出版社,2006:196-240.
[74]丛峰松.生物化学实验[M].上海:上海交通大学出版社,2005:83-84