昆白小鼠卵母细胞激活与孤雌胚体外发育
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摘要
昆白小鼠是我国自主繁殖的近交系小鼠,由于其遗传背景清楚、生殖周期短、环境适应能力强等优点,是国内良好的实验动物模型。本研究以昆白小鼠为研究对象,分离了子宫内膜上皮细胞,对卵母细胞的孤雌激活、孤雌胚胎的发育潜能进行了研究,探讨了影响孤雌激活和发育的几个因素,为孤雌胚胎干细胞的分离、建系提供一些基础。主要内容有以下几点:
1. 37℃下2.5 mg/mL胰蛋白酶+0.04%EDTA 30 min消化子宫内膜,结合刮取法,能获得活力高、数目多、细胞比较单一的子宫内膜上皮细胞,子宫内膜上皮细胞的活力随着细胞培养代数的增加而下降(噻唑蓝法检测)。
2. 7%乙醇刺激小鼠卵母细胞,激活5～7 min时其激活效果及孤雌胚发育较好,其桑囊胚发育率可达29.77%;
3. SrCl2激活小鼠卵母细胞时,6～10 mmol/L为最佳处理浓度,3～6 h为最佳激活时间,其桑囊胚发育率可达16.67%。
4.在复合激活中,7%乙醇激活5 min在联合2 mmol/L 6-DMAP +5μg/mLCB激活3 h效果最佳,其桑囊胚发育率高达35.77%。
5.添加15%FBS的CZB和mM16培养液均能较好地支持2-cell胚胎发育到囊胚(27.2%,26.3%)。同时,添加FBS的CZB和mM16组的囊胚发育率也均显著高于相应的
添加BSA组(p<0.01)。6. 15%FBS的CZB和mM16培养液进行孤雌胚培养时,胚胎在mM16培养液中仅有7.45%突破2-cell阻滞。但在CZB培养液中有41.66%能发育通过2-cell期并有29.17%到达桑囊胚。
7.昆明小鼠MⅡ卵母细胞激活后在含15%FBS的CZB培养液中可较好地克服2-cell阻滞并形成桑囊胚。在培养48 h添加1.1 mg/mL葡萄糖对胚胎的继续发育有利。
8.颗粒细胞、输卵管上皮细胞和小鼠子宫内膜上皮细胞与小鼠孤雌胚胎共培养24 h,共培养组胚胎与对照组大部分都能进行分裂。而从2-cell期进入4～8-cell期,CZB与颗粒细胞共培养组的胚胎发育无差异,但输卵管、子宫内膜上皮细胞共培养的胚胎要稍好一些(49.12%,48.23%)。CZB培养的桑囊胚发育率(32.99%)略低于其它三个共培养组的胚胎发育率(36.84%,38.71%,38.82%)(p<0.05)。因此,共培养细胞对孤雌胚的发育有一定的促进作用。
KB mouse, which is bred in our country by near inbred, has been a good trial animal model because of the well-known inheriting background, short reproduction cyc1e and the strong environmental adapt ability. In this experiment, we isolated endometrial epithelium cells from KB mouse, and studied the activation of mouse oocytes and culturing of parthenogenesis. We also discussed some factors of activation and development. Our work may be useful to further isolate and esTab. lish mouse parthenogenesis ES cell lines. The main contents are as follows:
1. Endometrial epithelium cells were isolated by the use of 2.5 mg/mLTrypsine + 0.04% EDTA in 37℃to digest the endometrial epithelium in 30 min, combined with scraping method. The vitality and amount of cells was the highest and the cells are more single-typed, the vitality of epithelium cells declined with the increase of passages (detected by MTT method).
2. Mouse oocytes were activated by 7% ethanol. After 5-7 min ,the activated effect and the parthenogenetic development were better than others, the development rates of over morula and blastocyst were up to 29.77%.
3. When mouse oocytes were activated by SrCl2, the optimum concentration was 6-10 mmol/L and the time of the optimum activated is 3-6 h, so the development rates of over morula and blastocyst was up to 16.67%.
4. In combined activation, mouse oocytes were activated 5 min by 7% ethanol, and was cultured 3 h into medium which combined with 2 mmol/L 6-DMAP +5μg/mLCB, the development rates of over morula and blastocyst were up to 35.77%.
5. CZB and mM16 medium containing 15%FBS supported optimum development from 2- cell embryos to blastocyst(27.2%,26.3%). In the meantime, compared with the development rates of blastocyst in CZB and mM16 medium containing 15%FBS with containing BSA,the difference were extreme.
6. For mouse parthenogenesis embryos, in CZB medium, 41.66% embryos prevented the 2- cell block and finally 29.17% embryos reached morula or blastocyst.But7.45% embryos developed to 3～4-cell embryos in mM16 medium, exhibiting an obvious“2-cell block".
7. Activared MⅡoocytes prevented the 2-cell block in CZB+15%FBS medium and developed to morula or blastocyst. The presence of 1.1mg/mL glucose in the medium after culturing 48 h improved the continuous development of embryos.
1. 37℃下2.5 mg/mL胰蛋白酶+0.04%EDTA 30 min消化子宫内膜,结合刮取法,能获得活力高、数目多、细胞比较单一的子宫内膜上皮细胞,子宫内膜上皮细胞的活力随着细胞培养代数的增加而下降(噻唑蓝法检测)。
2. 7%乙醇刺激小鼠卵母细胞,激活5～7 min时其激活效果及孤雌胚发育较好,其桑囊胚发育率可达29.77%;
3. SrCl2激活小鼠卵母细胞时,6～10 mmol/L为最佳处理浓度,3～6 h为最佳激活时间,其桑囊胚发育率可达16.67%。
4.在复合激活中,7%乙醇激活5 min在联合2 mmol/L 6-DMAP +5μg/mLCB激活3 h效果最佳,其桑囊胚发育率高达35.77%。
5.添加15%FBS的CZB和mM16培养液均能较好地支持2-cell胚胎发育到囊胚(27.2%,26.3%)。同时,添加FBS的CZB和mM16组的囊胚发育率也均显著高于相应的
添加BSA组(p<0.01)。6. 15%FBS的CZB和mM16培养液进行孤雌胚培养时,胚胎在mM16培养液中仅有7.45%突破2-cell阻滞。但在CZB培养液中有41.66%能发育通过2-cell期并有29.17%到达桑囊胚。
7.昆明小鼠MⅡ卵母细胞激活后在含15%FBS的CZB培养液中可较好地克服2-cell阻滞并形成桑囊胚。在培养48 h添加1.1 mg/mL葡萄糖对胚胎的继续发育有利。
8.颗粒细胞、输卵管上皮细胞和小鼠子宫内膜上皮细胞与小鼠孤雌胚胎共培养24 h,共培养组胚胎与对照组大部分都能进行分裂。而从2-cell期进入4～8-cell期,CZB与颗粒细胞共培养组的胚胎发育无差异,但输卵管、子宫内膜上皮细胞共培养的胚胎要稍好一些(49.12%,48.23%)。CZB培养的桑囊胚发育率(32.99%)略低于其它三个共培养组的胚胎发育率(36.84%,38.71%,38.82%)(p<0.05)。因此,共培养细胞对孤雌胚的发育有一定的促进作用。
KB mouse, which is bred in our country by near inbred, has been a good trial animal model because of the well-known inheriting background, short reproduction cyc1e and the strong environmental adapt ability. In this experiment, we isolated endometrial epithelium cells from KB mouse, and studied the activation of mouse oocytes and culturing of parthenogenesis. We also discussed some factors of activation and development. Our work may be useful to further isolate and esTab. lish mouse parthenogenesis ES cell lines. The main contents are as follows:
1. Endometrial epithelium cells were isolated by the use of 2.5 mg/mLTrypsine + 0.04% EDTA in 37℃to digest the endometrial epithelium in 30 min, combined with scraping method. The vitality and amount of cells was the highest and the cells are more single-typed, the vitality of epithelium cells declined with the increase of passages (detected by MTT method).
2. Mouse oocytes were activated by 7% ethanol. After 5-7 min ,the activated effect and the parthenogenetic development were better than others, the development rates of over morula and blastocyst were up to 29.77%.
3. When mouse oocytes were activated by SrCl2, the optimum concentration was 6-10 mmol/L and the time of the optimum activated is 3-6 h, so the development rates of over morula and blastocyst was up to 16.67%.
4. In combined activation, mouse oocytes were activated 5 min by 7% ethanol, and was cultured 3 h into medium which combined with 2 mmol/L 6-DMAP +5μg/mLCB, the development rates of over morula and blastocyst were up to 35.77%.
5. CZB and mM16 medium containing 15%FBS supported optimum development from 2- cell embryos to blastocyst(27.2%,26.3%). In the meantime, compared with the development rates of blastocyst in CZB and mM16 medium containing 15%FBS with containing BSA,the difference were extreme.
6. For mouse parthenogenesis embryos, in CZB medium, 41.66% embryos prevented the 2- cell block and finally 29.17% embryos reached morula or blastocyst.But7.45% embryos developed to 3～4-cell embryos in mM16 medium, exhibiting an obvious“2-cell block".
7. Activared MⅡoocytes prevented the 2-cell block in CZB+15%FBS medium and developed to morula or blastocyst. The presence of 1.1mg/mL glucose in the medium after culturing 48 h improved the continuous development of embryos.
引文
[1] Kono T,Obata Y,Wu Q,et al.Birth of parthenogenetic mice that can develop to adulthood[J]. Nature.2004,428:860-864.
[2] CibelliJB,Grant KA,Chapmankb,et al.Parthenogenetic stem cell sinnohuman primates[J].Science, 2002,295:55-56.
[3] 范必勤,邓满文.哺乳动物卵的激活与孤雌发育.生物工程进展[J], 1994,14(3):50-54.
[4] Niwa K,Takano R,Obata Y,et al.Nuclei of oocytes derived from mouse parthenogenetic embryos are competent to support development to term[J]. biolreprod,2004,104(1095):1-29.
[5] TKono.Genomic imprinting is a barrier to parthenogenesis in mammals[J].Cytogenet Genome Res, 2006,113(1-4):31-35.
[6] Beatty RA.Parthenogenesis and Polyploidyin Mammalian Development[J].London:Cambridge University Press,1937,36:13-15.
[7] Kaufman R H.Herpes genitalis treated by photodynamic inactivation of virus [J].Nature,1973,242:475-476.
[8] Iwamoto M,Onishi A,Fuchimoto D,et al.Low oxygen tension during in vitro maturation of porcine follicular oocytes improves parthenogenetic activation and subsequent development to the blastocyst stage[J].Theriogenology,2005,63(5):1277-1289.
[9] Gardner DK,Lane M.Differential regulation of mouse embryo development and viability by amino acids[J],J Reprod Fertil,1997,109:153.
[10] Gomez E,Tarin J J,Pellicer A.Oocyte matunration in human the role of gonadotropins and growth factors[J].Fertil Steril,1993,60(1):40-46.
[11] Lorenzo PL,Hlera MJ,HIera JC.Enhancement of cumulus expansion and nuclear maturation during bovine oocyte maturation in vitro by the addition of epidermal growth factor and insulin-like growth factor[J] .Reprod Fertil,1994,101:697-701.
[12] Krylov V,Kren R,Okada,et al.Effect of protein supplement source on porcine oocyte maturation and subsequent embryonic development after parthenogenetic activation[J].Folia Biol (Praha), 2005,51(2):29-33.
[13] 雷安民.山羊-牛异种细胞核移植研究[M].西北农林科技大学博士生论文,2004.
[14] 钱菊汾.家畜胚胎学[M].中国科学文化出版社,2003.
[15] Takakura I,Naito K,Iwamori N,et al.Inhibition of mitogen activated protein kinase activity induces parthenogenetic activation and increases cyclin B accumulation during porcine oocyte maturation[J].J Reprod Dev,2005,51(5): 617-26.
[16] Vincent C, Cheek TR, Johnson MH.Cell cycle progression of parthenogenetically activated mouse oocytes to interphase is dependent on the level of internal calcium[J].J Cell Sci,1992 Oct;103 ( Pt 2):389-96.
[17] incent C,Cheek TR,Johnson MH.Transient reactivation of CSF in parthenogenetic one-cell mouse embryos[J]. J Cell Sci,1992,103 (2):389-396.
[18] 胡 炜,汪亚平,朱作言.核移植后的细胞核再程序化机制研究进展[j].遗传学报,2003,30(5): 485-492.
[19] Polejaeva IA ,Chen S H,Vaught T D,et al.Cloned pigs produced by nuclear transfer from adult somatic cells[J ].N ature,2000,407(6800):862~890.
[20] Stice SL,Robl J M.Activation of mammalian oocytes by a factor obtained from rabbit sperm[J].Mol Reprod Dev,1990,25(3):272-280.
[21] Szabolcs T,Daniel H,Bernadette B,et al,Egg activation is the result of calcium signal summation in the mouse[J].Reproduction,2006,131:27-34.
[22] 李裕强 , 张涌 , 王新庄 . 乙醇和电场激活小鼠早期卵母细胞的研究 [J]. 西北农业大学学报.1999,7(2):58-61.
[23] Koo D B,Chae J I,Kim J S,et al.Inactivation of MPF and MAP kinase by single electrical stimulus for parthenogenetic development of porcine oocytes[J]. Mol Reprod Dev, 2005,72(4):542-549.
[24] Ebensperger C,BarrosC.Changes at the bamster oocyte surface from the germinal vesicle to ovulation[J].Gametc Res,1984,9:387-397.
[25] Carroll J,Swaon K.Spontaneous cytosolic calcium oscillations of mouse oocytes[j].J Biol Chem,1992,267:11196-11201.
[26] Li Chaojun,Wang bin,FanBiqin.The role of calcium oscillation in mouse oocyte during meiotic matulation[J].Metal Jons in BioMed,1994,411-415.
[27] Li Chaojun,FanBiqin.Changes in 3-dimensional distribution of mitochondria during meiotic division of mouse oocyte[J].Theriogenology,1997,48(1):33-34.
[28] Rark YS,Lin YC.Effect of epidermal growth tactor(EGF)and defined simple media in vitro bovine oocyte maturation and early embryonic development[j]. Theriogenology,1993,39 (3):475-484.
[29] O’neill GT,Rolfe LR, Kaufman MH .Developmental potential and chromosome coostitutionof strontium induced mouse parthengenones[J].Mol Reprod Dev.1991,30:214-219.
[30] 王斌,范必勤.兔卵母细胞的电刺激孤雌激活及体外发育[J].1994,25(6):507-512.
[31] SZ Liu, MX Jiang, LY Yan,et al.Parthenogenetic and nuclear transfer rabbit embryo development and apoptosis after activation treatments.Mol Reprod Dev, 2005,72(1):48-53.
[32] Vitullo A,etal.Develop Biol,1992,151:128-136.
[33] Cuthbertson KSR,Whittingham DG,Cobbold PH.Free Ca2+ increase in exponential phAsesDuring mouse oocytes activation[J].Nature,1981,294:754-757.
[34] FanBiqin .Oocyte meiotic maturation and development of parthenogentic and reconstituted embryos in mammal[J].JiangsuJ of Agr sci,1999,15(2):116-126.
[35] SF Ma,XY Liu,DQ Miao,et al Parthenogenetic activation of mouse oocytes by strontium chloride: a search for the best conditions[J]. Theriogenology,2005,64(5):1142-1157.
[36] Vitullo A.Develop Biol,1992,151:128-131.
[37] Alexander B,Coppola G,Berardino D Di,et al.The effect of 6-dimethylaminopurine (6-DMAP) and cycloheximide (CHX) on the development and chromosomal complement of sheep parthenogenetic and nuclear transfer embryos[J]. Mol Reprod Dev, 2006,73(1):20-30.
[38] 舒益民,庄广伦,周灿权等.应用钙离子载体A23187及6-DMAP对人卵母细胞进行孤雌激活[J].生殖医学杂志.2002, 11(3):145-148.
[39] Kline D,Kline J T. Regulation of intracellular calcium in the mouse egg: Evidence of inositol triosphate-induced calcium release, but not calcium-induced calcium release[J].Biol Reprod.1992, 50:193-203.
[40] Michael W.Calcium at Fertilization and in Early Development[J].Physiol Rev,2006,86: 25-88.
[41] L iu L,Ju J C,Yand X.Parthenogenetic development and protein patterns of newly maturated bovine oocytes after chemical activation[J].Mol Reprod Dev.1998,49(2):298-304.
[42] Machat y Z, Funahashi H,Mayes M A,et al.Effects of injecting calcium chloride into in vitro-maturated porcine oocytes[J]. Biol Reprod,1996,54(2):316-322.
[43] YJ Y,Park CS.Parthenogenetic development of porcine oocytes treated by ethanol, cycloheximide, cytochalasin B and 6-dimethylaminopurine[J]. Anim Reprod Sci, Apr 2005,86(3):297-304.
[44] Fissore RA,Gordo AC,WuHua.Activation of development in mammal:is there a role foe a sperm cytosolic facter?[J].Theriogenology,1998,49(1):43-53.
[45] 范必勤,邓满文.1994.哺乳动物卵的激活与孤雌发育[J].生物工程进展,14(3):50-54.
[46] Shuji Y, Koji N, Hisayo N, et al. Fertilization failure and oocyte activation[J]. Journal of Medical Investigation. 2000. 47:1-8. [47 ]刘红林,范必勤.哺乳动物卵母细胞孤雌发育研究[J].动物学杂志, 1997,32(4):55-58.
[48] Ozil J P.The parthenogenetic development of rabbit vtes after reptitive pulsatile electrical stimulation[J].Development,1990,109:117-127.
[49] Loi P,et al.Development of parthenogenetic and(lonedovine embrvos:effect of activation protocols [J].Biology of Reproduction,1998,58:1177-1187.
[50] Stevens LC.Totipotent cells of parthenogenetic origin in a chimaeric mouse [J]. Nature,1978,276(5685):266-267.
[51] Kaufman MH.Mammalian parthenogenesis in Early Mammalian Development Parthenogen etic Dtudies[J].London:Cambridge University Press,1982,1-19.
[52] FanBiqin .Oocyte meiotic maturation and development of parthenogentic and reconstituted embryos in mammal[J].JiangsuJ of Agr sci,1999,15(2):116-126.
[53] Solter D,Biczysko W,Graham C,et al.Ultrastructure of early development of mouse parthenogenones[J].J Exp Zool,1974,188(1): 1-23.
[54] 邓满齐,范必勤,刘永民.小鼠卵受精和人工激活过程中细胞质游离 Ca2+的变化及其在卵激活中的作用[J].动物学报,1996,42(1):80-86.
[55] 刘林,张忠诚,土水胜.绵羊个体成热卵母细胞的电激活及其在个体孤雌发育的研究[J].动物学报,1998,44 (4 ):74-81.
[56] 王新庄,张涌,张美佳.牛卵母细胞电激活参数的研究[J].西北农业大学报,1997,25 3 ):73-76.
[57] 杨索芳,石德顺.水牛卵母细胞孤雌激活方法的研究[J].西南农业大学学报,2000,13 (2):99-103.
[58] 李光鹏,孟庆刚,魏鹏,等.亚胺环己酮对猪卵母细胞人工孤雌激活作用的研究[J].畜牧兽医学报,2001,32(5):416-420.
[59] 舒益民,庄广伦,周灿权等.应用钙离子载体 A23187 及 6-甲基嘌呤对人卵母细胞进行孤雌激活[J].生殖医学杂志, 2002,11(3):145-148.
[60] Surani MA,Barton SC, Howlett SK, Influence of chromosomal determinants on development of androgenetic and parthenogenetic cells[J].Development,1988,103:171.
[61] Camb J.Econ.The unknown masterpiece: Marx's model of capital[J].Cambridge Journal of Economics,1998,22(3):297-324.
[62] Hashimoto N,Watanabe N,Furuta Y,et al.Parthenogenetic activation of oocytes in c-mos-deficient mice[J].Nature,1994,370(6484):68-71.
[63] Claudio S,Arturo B,Andrea B,et al,Parthenogenetic activation of mouse eggs by microinjection of a truncated c-kit tyrosine kinase present in spermatozoa[J].Development,1994, 124:2267-2274.
[64] 刘红林,范必勤,宗 卉,等.小鼠孤雌胚胎干细胞集落的建立[J].动物学报,1998,44(2):247-~248.
[65] Lin L,James R,Trimarchi,et al.Keefe Haploidy but Not Parthenogenetic Activation Leads to Increased Incidence of Apoptosis in Mouse Embryos[J].Biologyof reproduction,2002,66:204-210.
[66] Joanne L,Thorvaldsen,Andrew M,et al.Developmental Profile of H19 Differentially Methylated Domain Deletion Alleles Reveals Multiple Roles of the DMD in Regulating Allelic Expression and DNA Methylation at the Imprinted H19/Igf2 Locus[J].Mol Cell Biol.2006,26:1245-1258.
[67] Fujimoto A,Mitalipov S M.,Clepper L L,et al.Development of a monkey model for the study of primate genomic imprinting. Mol Hum Reprod,2005,11:413-422.
[68]Akihisa F,Shoukhrat M,Mitalipov,et al.WolfAberrant Genomic Imprinting in Rhesus Monkey Embryonic Stem Cells[J].Stem Cells,2006,24:595-603.
[69] 岳占碰,杨增明.哺乳动物基因印迹及其与胚胎发育的关系[J].动物学杂志, 1999, 34(4):56-59.
[70] Surani M A,Barton S C, Norris M. L. Development of Reconstituted Mouse Eggs Suggest Imprinting of the Genome during Gametogenesis[J].Nature ,1984,308:548-550.
[71] 雷呈祥.印记遗传[J].生物学通报,1994,29(12),8-10.
[72] Looijenga L H,Verkert A J,Groot N D,et al.H19 in Normal Development and Neoplasia[J]. Mol . Reprod. Dev ,1997 , 46 :419-439.
[73] Wise T L, Pravtcheva D D. Perinatal Lethality in H19 Enhancers2Igf2 Transgenic Mice[J]. Mol Reprod Dev,1997,48 :194-207.
[74] Surani M A, BartonS C, Norris M L. Development of Reconstituted Mouse Eggs Suggest Imprinting of the Genome during Gametogenesis[J].Nature,1984,308:548-550.
[75] Tomohiro K,Yusuke S,Yukiko K,et al.Mouse Parthenogenetic Embryos with Monoallelic H19 Expression Can Develop to Day 17.5 of Gestation[J].Developmental Biology,2002, 243(2):294-300.
[76] Qiong W, Takuya K, Manabu K.Regulated expression of two sets of paternally imprinted genes is necessary for mouse parthenogenetic development to term[J]. Reproduction,2006,131: 481-488.
[77] Lee S H,Lumelsky N,Studer L,et al. Efficient generation of mid brain and hindbrain neurons form mouse embryonic stem cells[J].Nat Biotechnol,2000,18(6):675-679.
[78] Burdon T, Smith A, Savatier P. Signalling cell cycle and pluripotency in embryonic stem cells[J].Trends Cell Biol.2002,12: 432-4381.
[79] Eiges R,Benvenisty N.A molecular view on pluripotent stem ce11s[J].FEBS, 2002,529:135-141.
[80] 孟国良,汤富酬,尚克刚,等.高效建立 129/ReJ, C57BL/6J 小鼠胚胎干细胞系的方法学探讨[J].生物工程学报,2002,18: 740-743.
[81] Chatot C L.Ziomek,C A.,Bavister B D.An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro1[J]. Reprod. Fert, 1989,86:679-688.
[82] Vitullo A D, OalJ P.Repetitive calcium stimuli drive meiotic resuption and pronuelear development duringoocyte acbvahnn[J].Dev Riol,1992,151:128.
[83] Wakavama T,Pcrrv A C F, ZnccottiM,et al.Full-tcrm development of mice from enucacatedl oocvtes injected with cumuluscell nuclei[J],Nuture ,1998,394(23):369-74.
[84] Parrington J,Swann K,Shevchenko V I,et al.Calcium oscillations in mammalian eggs triggered by a soluble sperm protern[J].Nature,1996, 379:364.
[85] Cuthbertson K S R,Vhittingham D G, Cobbold P H. Free Ca2+ increase in exponential phases during mouse oocyte ac-tivation[J]. Nature,1981,294:754.
[86] Kline D, Kline JT. Repetive calcium transients and the role of calcium in exocytesis and cell cvcle activation in the mouse egg[J],Biol dev,1992,149(1):80-89.
[87] Cuthbertson K S R,Whittingham D G,Cobbold P H. Free Ca2+ increase inexponential oocytes activation[J].1981,294:754-757.
[88]Tamashiro K L K,Wakavama T,Blanchard R T,et al.Postnatal growth and behavioral development of mice cloned from adult cumulus cells[J].Biol Reprod,2000,63(3):28-34.
[89] Raz T,Ben-yosef D,Shalgi R.Segregation of the pathway:leading to corticalreaction and cell cycle activation in the rat egg[J].Biol Reprod,1998,58:94-102.
[90] Hogben M.Parrington J,Shevchenko V,et al.Calcium oscillations, sperm factors and egg activation at fertilization[J].Mol Med,1998,76:548-554.
[91] Swan K, Parrington J.Mechanism of Ca2+ release at fertilization in mammals[J]. Experimantal zoology (Mol Dev Evol).1999; 285: 267-275.
[92] Liu CT,Chen CH,Cheng SP,et al.Parthenogenesis of rabbit oocytes activated by different stimuli[J]. Animal Reprod Sci.2002; 70: 267-276.
[93] Nakada K, Mizuno J.Intracellular calcium responses in bovine oocytes induced byspermatozoa and regents[J]. Theriogenology. 1998; 80:262-282. Sara M,Styliani Ma, Tom D.Antagonists of Myosin Light Chain Kinase and of Myosin II Inhibit Specific Events of Egg Activation in Fertilized Mouse Eggs[J].Biol Reprod,2006,74:169-176.
[94]Liu L,Ju JC,XZ.Differentialinactivation of maturation-promoting factor and mitogen-activated protein kinase following parthenogenetic activation of bovine oocytes[J]. BioReprod.1998:59:537-545.
[95] Rinaudo P,Massobrio M,Pepperell JR,et al. Dissociation between intracellular calcium elevation and development of human oocytes treated with calcium ionophore[J].fiertii Steril. 1997, 68: 1086-1092.
[96] Ben-Yosef D,Shalgi R.Oocyte activation: lessons from human infertility[J].Trends Mol Med.2001,7:163-169.
[97] Fan HY,Sun QY.Involvement of mitogen-activated protein kinase cascade during oocyte maturation and fertilization in mammals[J].Biol Reprod.2004,70:535-547.
[98] Sun QY,Lax Y,Rubinstein S,et al. Mitogen-activated protein kinase and cell cycle progression during mouse egg activation induced by various stimuli[J].Naturforsch,1999,54:285-294.
[99] Leal CLU, Liu L. Differential effects of kinase inhibitor and electrical stimulus on activationand histone H1 kinase activity in pig oocytes[J]. Animal Reprod Sci,1998,52:51-61.
[100] Liu L,Yang XZ.Interplay of maturation-promoting factor and mitogen-activated protein kinase inactivation during metaphase-to interphase transition of activated bovine oocytes[J].Biol Reprod.1999,61:1-7.
[101] Koo DB, Chae JI, Kim JS,et al.Inactivation of MPF and MAP kinase by single electrical stimulus for parthenogenetic development of porcine oocytes.Mol Reprod Dev, 2005,72(4): 542-549.
[102] Nakasaka H,Yamano S,Hinokio K,et al. Effective activation method with A23187 andpuromycin to produce haploid parthenogenones from freshly ovulated mouse oocytes[J]. Zygote,2000,8:203-208.
[103] 陈北亨.兽医产科学[M].兰州:农业出版社,1988:5-8.
[104] 张 丽 凤 , 苏 应 宽 , 盖 凌 , 等 . 人 子 宫 内 膜 基 质 细 胞 及 上 皮 细 胞 的 分 离 [J]. 生 殖 医 学 杂志,1998,(4):229-231.
[105] 靳 亚 平 , 王 爱 华 , 武 浩 , 等 . 小 鼠 和 山 羊 子 宫 内 膜 淋 巴 细 胞 的 制 备 [J]. 动 物 医 学 进展,2000,21(3):45-45.
[106] 陈秀荔,靳亚平,利光辉,等.孕早期家兔子宫内膜细胞的分离培养与形态观察[J].西北农林科技大学学报(自然科学版),2004,32(6):1-4.
[107] 曾国庆,曹宇静.小鼠胚胎与子宫单层细胞共培养的研究.动物学报[J],1996,42(2):212-216.
[108] 钟 瑜,潘善培,张春雪,等.人输卵管上皮细胞共培养系统对小鼠胚胎体外发育的影响[J].生殖医学杂志,1996,5(3):162-165.
[109] Henriksen T,Tanbo T,Abyholn T h,etal.Epithelial cells from human fall opian tube in culture[J].Human Reprod,1990,5:25-29.
[110] Eyestone W H,First N L.Co-culture of early cattle embryos to the blastocyst stage with oviductal tissue orin conditional medium[J].J Reprod Fertil,1989,85:715-720.
[111] 林 静,陈奕权.输卵管上皮细胞的培养及生物学特性[J].福建医科大学学报,1997,31(2) :152-155.
[112] 钟 瑜,张春雪,潘善培.建立人输卵管上皮细胞无血清培养[J].暨南大学学报,1998,19:31-35.
[113] Walton LA, Siegfried JM,Nelson KG,et al. Endometrial stromal cells in culture: an attempt to understand the genesis and biologic activity of uterine sarcomas[J].Gynecol Oncol,1986,24:247-252.
[114] 谢厚祥译,日木 NAME 学会编.哺乳动物的发育工程[J].湖南:湖南科学技术出版社,1986.
[115] Brigid H, Prank C,Elizabeth L.Manipulatinn the Mouse embryo.New york[J].Cold Spring Harbor Labo-ratorv Press,1986:107.
[116] Xu K P,Yadav B R,Rorie R W,et al.Development and viability of bovine embryos derived from oocytes ma-turcd and fertilised in vitro and co-cultured with bovine oviducal epithelial cells[J].J Reprod Fert,1992,94:33.
[117] Chatot C L,Lewyis J L,Torres I,et al. Development of 1-cell embryos from different strains of mice in CZB medium[J].biolony of Reprod,1990,42:432.
[118] Dandikar P V,Clase R H. Development of mouse embryos in vitro is affected by strain and culture mediums[J] .Gamete Res,1987,17:279.
[119] Chatot C L,ziomijk C A, havistijr h D, et al. An improved medium supports development of random- bred 1-ell mouse embryos in vitro[J]. Reprod Fert,1989,8l:179.
[120] 庞也非,旭日干.昆明小鼠卵子的体外受精及发育的研究[J].细胞生物学杂志,1990,12(4):175.
[121] 刘莹,武迪迪,宗志宏.昆明系小鼠体外受精方法及一种新培养基的应用[J].中国医科大学学报.1999 28(1):8-10.
[122] 沈 虹,刘传聚,顾 正,等.克服早胚发育阻断的兔输卵管因子“DPF- 1”的初步研究[J].实验生物学报,1996,29( 1):403.
[123] 李逸平,沈虹,顾正,等.推进早期胚胎发育正常调控过渡的输卵管细胞因子的初步研究[J].动物学研究,1997,18(1):85.
[124] Bavister B D.Co-culture for embryo development: is it really necessary?[J].Human Reprod, 1992,7(10):1339.
[125] 庞也非,李 东,旭日干.昆明小鼠卵子的体外受精及其新培养系统的建立[J].汕头大学医学院学报,1995,1:17-20.
[126] Iyengar M G,Iyengar C W L,Chen H Y,e1 ad.Expression of creation kinaseisoenzyme during oogenesis and embryogenesis in mouse[J].Dev Biol,1983,96:262-268.
[127] Chatot C L, Ziomljk C A, havistijr h D, et al. An improved medium supports development of random- bred 1-cell mouse embryos in vitro[J].J Reprod fert,1989,8l:179.
[128] Chatot C L,Tascar J A, Ziomljk C.Glutamine uptake and utilization by preimplantation mouse embryos in CZB medium[J].J Reprod Fertil,1990,89:335.
[129] Jackson K V,Kiessling A A.Fertilization of cleavage of mouse oocytes exposed to conditions of human oocyteretrival for in vitro fertilizations[J].Fertil Steril,1989,51:675
[130] Gandolfi F.Brevini TAL.Rechardson L,et al.characterization of proteins secreted by sheep oviductepithelialcells and their function in embryonic development[J]. Devlopment,1989,106:303.
[131] Kapnr PPJohnson LV.Selective sequestration of oviductal fluid glycoprotion in the perivitelline space of and embryos[J]. J Exp Zool,1986, 238:249.
[2] CibelliJB,Grant KA,Chapmankb,et al.Parthenogenetic stem cell sinnohuman primates[J].Science, 2002,295:55-56.
[3] 范必勤,邓满文.哺乳动物卵的激活与孤雌发育.生物工程进展[J], 1994,14(3):50-54.
[4] Niwa K,Takano R,Obata Y,et al.Nuclei of oocytes derived from mouse parthenogenetic embryos are competent to support development to term[J]. biolreprod,2004,104(1095):1-29.
[5] TKono.Genomic imprinting is a barrier to parthenogenesis in mammals[J].Cytogenet Genome Res, 2006,113(1-4):31-35.
[6] Beatty RA.Parthenogenesis and Polyploidyin Mammalian Development[J].London:Cambridge University Press,1937,36:13-15.
[7] Kaufman R H.Herpes genitalis treated by photodynamic inactivation of virus [J].Nature,1973,242:475-476.
[8] Iwamoto M,Onishi A,Fuchimoto D,et al.Low oxygen tension during in vitro maturation of porcine follicular oocytes improves parthenogenetic activation and subsequent development to the blastocyst stage[J].Theriogenology,2005,63(5):1277-1289.
[9] Gardner DK,Lane M.Differential regulation of mouse embryo development and viability by amino acids[J],J Reprod Fertil,1997,109:153.
[10] Gomez E,Tarin J J,Pellicer A.Oocyte matunration in human the role of gonadotropins and growth factors[J].Fertil Steril,1993,60(1):40-46.
[11] Lorenzo PL,Hlera MJ,HIera JC.Enhancement of cumulus expansion and nuclear maturation during bovine oocyte maturation in vitro by the addition of epidermal growth factor and insulin-like growth factor[J] .Reprod Fertil,1994,101:697-701.
[12] Krylov V,Kren R,Okada,et al.Effect of protein supplement source on porcine oocyte maturation and subsequent embryonic development after parthenogenetic activation[J].Folia Biol (Praha), 2005,51(2):29-33.
[13] 雷安民.山羊-牛异种细胞核移植研究[M].西北农林科技大学博士生论文,2004.
[14] 钱菊汾.家畜胚胎学[M].中国科学文化出版社,2003.
[15] Takakura I,Naito K,Iwamori N,et al.Inhibition of mitogen activated protein kinase activity induces parthenogenetic activation and increases cyclin B accumulation during porcine oocyte maturation[J].J Reprod Dev,2005,51(5): 617-26.
[16] Vincent C, Cheek TR, Johnson MH.Cell cycle progression of parthenogenetically activated mouse oocytes to interphase is dependent on the level of internal calcium[J].J Cell Sci,1992 Oct;103 ( Pt 2):389-96.
[17] incent C,Cheek TR,Johnson MH.Transient reactivation of CSF in parthenogenetic one-cell mouse embryos[J]. J Cell Sci,1992,103 (2):389-396.
[18] 胡 炜,汪亚平,朱作言.核移植后的细胞核再程序化机制研究进展[j].遗传学报,2003,30(5): 485-492.
[19] Polejaeva IA ,Chen S H,Vaught T D,et al.Cloned pigs produced by nuclear transfer from adult somatic cells[J ].N ature,2000,407(6800):862~890.
[20] Stice SL,Robl J M.Activation of mammalian oocytes by a factor obtained from rabbit sperm[J].Mol Reprod Dev,1990,25(3):272-280.
[21] Szabolcs T,Daniel H,Bernadette B,et al,Egg activation is the result of calcium signal summation in the mouse[J].Reproduction,2006,131:27-34.
[22] 李裕强 , 张涌 , 王新庄 . 乙醇和电场激活小鼠早期卵母细胞的研究 [J]. 西北农业大学学报.1999,7(2):58-61.
[23] Koo D B,Chae J I,Kim J S,et al.Inactivation of MPF and MAP kinase by single electrical stimulus for parthenogenetic development of porcine oocytes[J]. Mol Reprod Dev, 2005,72(4):542-549.
[24] Ebensperger C,BarrosC.Changes at the bamster oocyte surface from the germinal vesicle to ovulation[J].Gametc Res,1984,9:387-397.
[25] Carroll J,Swaon K.Spontaneous cytosolic calcium oscillations of mouse oocytes[j].J Biol Chem,1992,267:11196-11201.
[26] Li Chaojun,Wang bin,FanBiqin.The role of calcium oscillation in mouse oocyte during meiotic matulation[J].Metal Jons in BioMed,1994,411-415.
[27] Li Chaojun,FanBiqin.Changes in 3-dimensional distribution of mitochondria during meiotic division of mouse oocyte[J].Theriogenology,1997,48(1):33-34.
[28] Rark YS,Lin YC.Effect of epidermal growth tactor(EGF)and defined simple media in vitro bovine oocyte maturation and early embryonic development[j]. Theriogenology,1993,39 (3):475-484.
[29] O’neill GT,Rolfe LR, Kaufman MH .Developmental potential and chromosome coostitutionof strontium induced mouse parthengenones[J].Mol Reprod Dev.1991,30:214-219.
[30] 王斌,范必勤.兔卵母细胞的电刺激孤雌激活及体外发育[J].1994,25(6):507-512.
[31] SZ Liu, MX Jiang, LY Yan,et al.Parthenogenetic and nuclear transfer rabbit embryo development and apoptosis after activation treatments.Mol Reprod Dev, 2005,72(1):48-53.
[32] Vitullo A,etal.Develop Biol,1992,151:128-136.
[33] Cuthbertson KSR,Whittingham DG,Cobbold PH.Free Ca2+ increase in exponential phAsesDuring mouse oocytes activation[J].Nature,1981,294:754-757.
[34] FanBiqin .Oocyte meiotic maturation and development of parthenogentic and reconstituted embryos in mammal[J].JiangsuJ of Agr sci,1999,15(2):116-126.
[35] SF Ma,XY Liu,DQ Miao,et al Parthenogenetic activation of mouse oocytes by strontium chloride: a search for the best conditions[J]. Theriogenology,2005,64(5):1142-1157.
[36] Vitullo A.Develop Biol,1992,151:128-131.
[37] Alexander B,Coppola G,Berardino D Di,et al.The effect of 6-dimethylaminopurine (6-DMAP) and cycloheximide (CHX) on the development and chromosomal complement of sheep parthenogenetic and nuclear transfer embryos[J]. Mol Reprod Dev, 2006,73(1):20-30.
[38] 舒益民,庄广伦,周灿权等.应用钙离子载体A23187及6-DMAP对人卵母细胞进行孤雌激活[J].生殖医学杂志.2002, 11(3):145-148.
[39] Kline D,Kline J T. Regulation of intracellular calcium in the mouse egg: Evidence of inositol triosphate-induced calcium release, but not calcium-induced calcium release[J].Biol Reprod.1992, 50:193-203.
[40] Michael W.Calcium at Fertilization and in Early Development[J].Physiol Rev,2006,86: 25-88.
[41] L iu L,Ju J C,Yand X.Parthenogenetic development and protein patterns of newly maturated bovine oocytes after chemical activation[J].Mol Reprod Dev.1998,49(2):298-304.
[42] Machat y Z, Funahashi H,Mayes M A,et al.Effects of injecting calcium chloride into in vitro-maturated porcine oocytes[J]. Biol Reprod,1996,54(2):316-322.
[43] YJ Y,Park CS.Parthenogenetic development of porcine oocytes treated by ethanol, cycloheximide, cytochalasin B and 6-dimethylaminopurine[J]. Anim Reprod Sci, Apr 2005,86(3):297-304.
[44] Fissore RA,Gordo AC,WuHua.Activation of development in mammal:is there a role foe a sperm cytosolic facter?[J].Theriogenology,1998,49(1):43-53.
[45] 范必勤,邓满文.1994.哺乳动物卵的激活与孤雌发育[J].生物工程进展,14(3):50-54.
[46] Shuji Y, Koji N, Hisayo N, et al. Fertilization failure and oocyte activation[J]. Journal of Medical Investigation. 2000. 47:1-8. [47 ]刘红林,范必勤.哺乳动物卵母细胞孤雌发育研究[J].动物学杂志, 1997,32(4):55-58.
[48] Ozil J P.The parthenogenetic development of rabbit vtes after reptitive pulsatile electrical stimulation[J].Development,1990,109:117-127.
[49] Loi P,et al.Development of parthenogenetic and(lonedovine embrvos:effect of activation protocols [J].Biology of Reproduction,1998,58:1177-1187.
[50] Stevens LC.Totipotent cells of parthenogenetic origin in a chimaeric mouse [J]. Nature,1978,276(5685):266-267.
[51] Kaufman MH.Mammalian parthenogenesis in Early Mammalian Development Parthenogen etic Dtudies[J].London:Cambridge University Press,1982,1-19.
[52] FanBiqin .Oocyte meiotic maturation and development of parthenogentic and reconstituted embryos in mammal[J].JiangsuJ of Agr sci,1999,15(2):116-126.
[53] Solter D,Biczysko W,Graham C,et al.Ultrastructure of early development of mouse parthenogenones[J].J Exp Zool,1974,188(1): 1-23.
[54] 邓满齐,范必勤,刘永民.小鼠卵受精和人工激活过程中细胞质游离 Ca2+的变化及其在卵激活中的作用[J].动物学报,1996,42(1):80-86.
[55] 刘林,张忠诚,土水胜.绵羊个体成热卵母细胞的电激活及其在个体孤雌发育的研究[J].动物学报,1998,44 (4 ):74-81.
[56] 王新庄,张涌,张美佳.牛卵母细胞电激活参数的研究[J].西北农业大学报,1997,25 3 ):73-76.
[57] 杨索芳,石德顺.水牛卵母细胞孤雌激活方法的研究[J].西南农业大学学报,2000,13 (2):99-103.
[58] 李光鹏,孟庆刚,魏鹏,等.亚胺环己酮对猪卵母细胞人工孤雌激活作用的研究[J].畜牧兽医学报,2001,32(5):416-420.
[59] 舒益民,庄广伦,周灿权等.应用钙离子载体 A23187 及 6-甲基嘌呤对人卵母细胞进行孤雌激活[J].生殖医学杂志, 2002,11(3):145-148.
[60] Surani MA,Barton SC, Howlett SK, Influence of chromosomal determinants on development of androgenetic and parthenogenetic cells[J].Development,1988,103:171.
[61] Camb J.Econ.The unknown masterpiece: Marx's model of capital[J].Cambridge Journal of Economics,1998,22(3):297-324.
[62] Hashimoto N,Watanabe N,Furuta Y,et al.Parthenogenetic activation of oocytes in c-mos-deficient mice[J].Nature,1994,370(6484):68-71.
[63] Claudio S,Arturo B,Andrea B,et al,Parthenogenetic activation of mouse eggs by microinjection of a truncated c-kit tyrosine kinase present in spermatozoa[J].Development,1994, 124:2267-2274.
[64] 刘红林,范必勤,宗 卉,等.小鼠孤雌胚胎干细胞集落的建立[J].动物学报,1998,44(2):247-~248.
[65] Lin L,James R,Trimarchi,et al.Keefe Haploidy but Not Parthenogenetic Activation Leads to Increased Incidence of Apoptosis in Mouse Embryos[J].Biologyof reproduction,2002,66:204-210.
[66] Joanne L,Thorvaldsen,Andrew M,et al.Developmental Profile of H19 Differentially Methylated Domain Deletion Alleles Reveals Multiple Roles of the DMD in Regulating Allelic Expression and DNA Methylation at the Imprinted H19/Igf2 Locus[J].Mol Cell Biol.2006,26:1245-1258.
[67] Fujimoto A,Mitalipov S M.,Clepper L L,et al.Development of a monkey model for the study of primate genomic imprinting. Mol Hum Reprod,2005,11:413-422.
[68]Akihisa F,Shoukhrat M,Mitalipov,et al.WolfAberrant Genomic Imprinting in Rhesus Monkey Embryonic Stem Cells[J].Stem Cells,2006,24:595-603.
[69] 岳占碰,杨增明.哺乳动物基因印迹及其与胚胎发育的关系[J].动物学杂志, 1999, 34(4):56-59.
[70] Surani M A,Barton S C, Norris M. L. Development of Reconstituted Mouse Eggs Suggest Imprinting of the Genome during Gametogenesis[J].Nature ,1984,308:548-550.
[71] 雷呈祥.印记遗传[J].生物学通报,1994,29(12),8-10.
[72] Looijenga L H,Verkert A J,Groot N D,et al.H19 in Normal Development and Neoplasia[J]. Mol . Reprod. Dev ,1997 , 46 :419-439.
[73] Wise T L, Pravtcheva D D. Perinatal Lethality in H19 Enhancers2Igf2 Transgenic Mice[J]. Mol Reprod Dev,1997,48 :194-207.
[74] Surani M A, BartonS C, Norris M L. Development of Reconstituted Mouse Eggs Suggest Imprinting of the Genome during Gametogenesis[J].Nature,1984,308:548-550.
[75] Tomohiro K,Yusuke S,Yukiko K,et al.Mouse Parthenogenetic Embryos with Monoallelic H19 Expression Can Develop to Day 17.5 of Gestation[J].Developmental Biology,2002, 243(2):294-300.
[76] Qiong W, Takuya K, Manabu K.Regulated expression of two sets of paternally imprinted genes is necessary for mouse parthenogenetic development to term[J]. Reproduction,2006,131: 481-488.
[77] Lee S H,Lumelsky N,Studer L,et al. Efficient generation of mid brain and hindbrain neurons form mouse embryonic stem cells[J].Nat Biotechnol,2000,18(6):675-679.
[78] Burdon T, Smith A, Savatier P. Signalling cell cycle and pluripotency in embryonic stem cells[J].Trends Cell Biol.2002,12: 432-4381.
[79] Eiges R,Benvenisty N.A molecular view on pluripotent stem ce11s[J].FEBS, 2002,529:135-141.
[80] 孟国良,汤富酬,尚克刚,等.高效建立 129/ReJ, C57BL/6J 小鼠胚胎干细胞系的方法学探讨[J].生物工程学报,2002,18: 740-743.
[81] Chatot C L.Ziomek,C A.,Bavister B D.An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro1[J]. Reprod. Fert, 1989,86:679-688.
[82] Vitullo A D, OalJ P.Repetitive calcium stimuli drive meiotic resuption and pronuelear development duringoocyte acbvahnn[J].Dev Riol,1992,151:128.
[83] Wakavama T,Pcrrv A C F, ZnccottiM,et al.Full-tcrm development of mice from enucacatedl oocvtes injected with cumuluscell nuclei[J],Nuture ,1998,394(23):369-74.
[84] Parrington J,Swann K,Shevchenko V I,et al.Calcium oscillations in mammalian eggs triggered by a soluble sperm protern[J].Nature,1996, 379:364.
[85] Cuthbertson K S R,Vhittingham D G, Cobbold P H. Free Ca2+ increase in exponential phases during mouse oocyte ac-tivation[J]. Nature,1981,294:754.
[86] Kline D, Kline JT. Repetive calcium transients and the role of calcium in exocytesis and cell cvcle activation in the mouse egg[J],Biol dev,1992,149(1):80-89.
[87] Cuthbertson K S R,Whittingham D G,Cobbold P H. Free Ca2+ increase inexponential oocytes activation[J].1981,294:754-757.
[88]Tamashiro K L K,Wakavama T,Blanchard R T,et al.Postnatal growth and behavioral development of mice cloned from adult cumulus cells[J].Biol Reprod,2000,63(3):28-34.
[89] Raz T,Ben-yosef D,Shalgi R.Segregation of the pathway:leading to corticalreaction and cell cycle activation in the rat egg[J].Biol Reprod,1998,58:94-102.
[90] Hogben M.Parrington J,Shevchenko V,et al.Calcium oscillations, sperm factors and egg activation at fertilization[J].Mol Med,1998,76:548-554.
[91] Swan K, Parrington J.Mechanism of Ca2+ release at fertilization in mammals[J]. Experimantal zoology (Mol Dev Evol).1999; 285: 267-275.
[92] Liu CT,Chen CH,Cheng SP,et al.Parthenogenesis of rabbit oocytes activated by different stimuli[J]. Animal Reprod Sci.2002; 70: 267-276.
[93] Nakada K, Mizuno J.Intracellular calcium responses in bovine oocytes induced byspermatozoa and regents[J]. Theriogenology. 1998; 80:262-282. Sara M,Styliani Ma, Tom D.Antagonists of Myosin Light Chain Kinase and of Myosin II Inhibit Specific Events of Egg Activation in Fertilized Mouse Eggs[J].Biol Reprod,2006,74:169-176.
[94]Liu L,Ju JC,XZ.Differentialinactivation of maturation-promoting factor and mitogen-activated protein kinase following parthenogenetic activation of bovine oocytes[J]. BioReprod.1998:59:537-545.
[95] Rinaudo P,Massobrio M,Pepperell JR,et al. Dissociation between intracellular calcium elevation and development of human oocytes treated with calcium ionophore[J].fiertii Steril. 1997, 68: 1086-1092.
[96] Ben-Yosef D,Shalgi R.Oocyte activation: lessons from human infertility[J].Trends Mol Med.2001,7:163-169.
[97] Fan HY,Sun QY.Involvement of mitogen-activated protein kinase cascade during oocyte maturation and fertilization in mammals[J].Biol Reprod.2004,70:535-547.
[98] Sun QY,Lax Y,Rubinstein S,et al. Mitogen-activated protein kinase and cell cycle progression during mouse egg activation induced by various stimuli[J].Naturforsch,1999,54:285-294.
[99] Leal CLU, Liu L. Differential effects of kinase inhibitor and electrical stimulus on activationand histone H1 kinase activity in pig oocytes[J]. Animal Reprod Sci,1998,52:51-61.
[100] Liu L,Yang XZ.Interplay of maturation-promoting factor and mitogen-activated protein kinase inactivation during metaphase-to interphase transition of activated bovine oocytes[J].Biol Reprod.1999,61:1-7.
[101] Koo DB, Chae JI, Kim JS,et al.Inactivation of MPF and MAP kinase by single electrical stimulus for parthenogenetic development of porcine oocytes.Mol Reprod Dev, 2005,72(4): 542-549.
[102] Nakasaka H,Yamano S,Hinokio K,et al. Effective activation method with A23187 andpuromycin to produce haploid parthenogenones from freshly ovulated mouse oocytes[J]. Zygote,2000,8:203-208.
[103] 陈北亨.兽医产科学[M].兰州:农业出版社,1988:5-8.
[104] 张 丽 凤 , 苏 应 宽 , 盖 凌 , 等 . 人 子 宫 内 膜 基 质 细 胞 及 上 皮 细 胞 的 分 离 [J]. 生 殖 医 学 杂志,1998,(4):229-231.
[105] 靳 亚 平 , 王 爱 华 , 武 浩 , 等 . 小 鼠 和 山 羊 子 宫 内 膜 淋 巴 细 胞 的 制 备 [J]. 动 物 医 学 进展,2000,21(3):45-45.
[106] 陈秀荔,靳亚平,利光辉,等.孕早期家兔子宫内膜细胞的分离培养与形态观察[J].西北农林科技大学学报(自然科学版),2004,32(6):1-4.
[107] 曾国庆,曹宇静.小鼠胚胎与子宫单层细胞共培养的研究.动物学报[J],1996,42(2):212-216.
[108] 钟 瑜,潘善培,张春雪,等.人输卵管上皮细胞共培养系统对小鼠胚胎体外发育的影响[J].生殖医学杂志,1996,5(3):162-165.
[109] Henriksen T,Tanbo T,Abyholn T h,etal.Epithelial cells from human fall opian tube in culture[J].Human Reprod,1990,5:25-29.
[110] Eyestone W H,First N L.Co-culture of early cattle embryos to the blastocyst stage with oviductal tissue orin conditional medium[J].J Reprod Fertil,1989,85:715-720.
[111] 林 静,陈奕权.输卵管上皮细胞的培养及生物学特性[J].福建医科大学学报,1997,31(2) :152-155.
[112] 钟 瑜,张春雪,潘善培.建立人输卵管上皮细胞无血清培养[J].暨南大学学报,1998,19:31-35.
[113] Walton LA, Siegfried JM,Nelson KG,et al. Endometrial stromal cells in culture: an attempt to understand the genesis and biologic activity of uterine sarcomas[J].Gynecol Oncol,1986,24:247-252.
[114] 谢厚祥译,日木 NAME 学会编.哺乳动物的发育工程[J].湖南:湖南科学技术出版社,1986.
[115] Brigid H, Prank C,Elizabeth L.Manipulatinn the Mouse embryo.New york[J].Cold Spring Harbor Labo-ratorv Press,1986:107.
[116] Xu K P,Yadav B R,Rorie R W,et al.Development and viability of bovine embryos derived from oocytes ma-turcd and fertilised in vitro and co-cultured with bovine oviducal epithelial cells[J].J Reprod Fert,1992,94:33.
[117] Chatot C L,Lewyis J L,Torres I,et al. Development of 1-cell embryos from different strains of mice in CZB medium[J].biolony of Reprod,1990,42:432.
[118] Dandikar P V,Clase R H. Development of mouse embryos in vitro is affected by strain and culture mediums[J] .Gamete Res,1987,17:279.
[119] Chatot C L,ziomijk C A, havistijr h D, et al. An improved medium supports development of random- bred 1-ell mouse embryos in vitro[J]. Reprod Fert,1989,8l:179.
[120] 庞也非,旭日干.昆明小鼠卵子的体外受精及发育的研究[J].细胞生物学杂志,1990,12(4):175.
[121] 刘莹,武迪迪,宗志宏.昆明系小鼠体外受精方法及一种新培养基的应用[J].中国医科大学学报.1999 28(1):8-10.
[122] 沈 虹,刘传聚,顾 正,等.克服早胚发育阻断的兔输卵管因子“DPF- 1”的初步研究[J].实验生物学报,1996,29( 1):403.
[123] 李逸平,沈虹,顾正,等.推进早期胚胎发育正常调控过渡的输卵管细胞因子的初步研究[J].动物学研究,1997,18(1):85.
[124] Bavister B D.Co-culture for embryo development: is it really necessary?[J].Human Reprod, 1992,7(10):1339.
[125] 庞也非,李 东,旭日干.昆明小鼠卵子的体外受精及其新培养系统的建立[J].汕头大学医学院学报,1995,1:17-20.
[126] Iyengar M G,Iyengar C W L,Chen H Y,e1 ad.Expression of creation kinaseisoenzyme during oogenesis and embryogenesis in mouse[J].Dev Biol,1983,96:262-268.
[127] Chatot C L, Ziomljk C A, havistijr h D, et al. An improved medium supports development of random- bred 1-cell mouse embryos in vitro[J].J Reprod fert,1989,8l:179.
[128] Chatot C L,Tascar J A, Ziomljk C.Glutamine uptake and utilization by preimplantation mouse embryos in CZB medium[J].J Reprod Fertil,1990,89:335.
[129] Jackson K V,Kiessling A A.Fertilization of cleavage of mouse oocytes exposed to conditions of human oocyteretrival for in vitro fertilizations[J].Fertil Steril,1989,51:675
[130] Gandolfi F.Brevini TAL.Rechardson L,et al.characterization of proteins secreted by sheep oviductepithelialcells and their function in embryonic development[J]. Devlopment,1989,106:303.
[131] Kapnr PPJohnson LV.Selective sequestration of oviductal fluid glycoprotion in the perivitelline space of and embryos[J]. J Exp Zool,1986, 238:249.