麦草原料生物预处理与酚类化合物生物降解动力学研究
|
摘要
本文对造纸行业中具有较好应用前景的真菌门担子菌纲的白腐菌代表性菌株黄孢原毛平革菌("Phanerochaete chrysosprium"以下简称"P.chrysosprium")在麦草原料预处理中的应用以及废水生物处理等内容进行了较为系统的研究。课题涉及生物工程、制浆造纸工程以及环境化学与计算机技术应用等多学科内容，其成果对于利用生物技术处理纤维原料，减少制浆化学品消耗以及漂白废水污染控制工作具有重要的指导意义。
利用P. chrysosprium处理麦草原料，采用正交回归实验设计方法确定碳源(葡萄糖)、氮源(硝酸铵)、硫酸锰等对生物处理效果具有显著影响的因素水平最优化条件分别为(g/L)：27.22、0.06、0.20，作为麦草化学制浆预处理时间以6-12天为宜。根据最优化条件进行了碱法浆蒸煮试验，对比研究了生物预处理对麦草制浆的影响。结果表明：在用碱量8％--14％范围内，经过经过5天生物预处理的原料与未经处理的原料相比，在相同卡伯值和成纸强度条件下，用碱量降低2％。红外谱图分析结果显示，麦草木素生物降解是生物酶催化作用下的氧化反应，其结果C_α发生氧化、β-0-4键氧化裂解，导致木素大分子断裂溶出。
利用P. chrysosprium处理E段漂白废水(COD为389lmg/L)5天时COD去除率为71.8％、脱色率为65％。论文对密闭式分光光度法测定漂白废水COD方法进行了研究，确定最佳测定波长为430nm。t检验结果表明，该方法与标准COD_(cr)测定结果高度显著相关。实际应用中，该方法测定成本低，特别适宜批量试样同时测定，有利于消除系统误差。
对生物处理前后E段废水GC-MS检测结果证明，白腐菌P.chrysosprium处理漂白废水不产生二次污染；造成E段废水色度
的主要污染物为各种长碳链烷烃、烯烃以及苯基酯类、醇类化合
物，在菌体对数生长阶段可大量去除。
利用 olterra模型建立了 P Chwompum处理2，4一二氯酚模
拟废水动力学方程。通过动力学分析定量探讨了有毒化合物作用
下的菌体生长与底物降解关系。试验结果证实Volterra模型较好地
反映了有毒化合物对生物降解过程的抑制作用，较为精确地描述
了降解体系中菌体生长过程。动力学方程模拟结果与试验数据经
Pearson检验高度显著相关。动力学研究表明氯代酚的批次处理试
验中，生物吸附作用显著影响底物去除反应历程，这一特性在反
应器设计及氯代酚类有毒污染物生物降解机理研究中应给予重
视。在试验条件下使用P Chlysosporium处理2，4一二氯酚120h，
去除率＞99％。
在本文的最后部分，利用分子连接性指数（MCI）描述酚类化
合物结构，建立了人工神经网络（Artificial Neural Net，ANN）
模型预测酚类化合物可生物降解性能。使用BP算法对试验任选的
两组化合物的预测结果正确率均大于80％，预测结果显示了人工
神经元网络模型对于各种酚类化合物的可生物降解性具有良好的
预测能力，体现了网络模型自适应能力对于变量间相互关系和物
理意义不明的多变量非线性函数具有良好的逼近性能。
The research is focused on the application of white rot fungi in pulping and paper making field. In this paper, Phanerochaete chrysosprium (P. chrysosprium), a kind of representative white rot fungi, is used in straw bio-pretreatment and effluent bio-treatment respectively to decline the consumption of chemicals in pulp and the pollution of bleaching effluent.
The nutritional factors were optimized for better lignin bio-degradation with Orthogonal Regressive Analysis Method, i.e., carbon source (glucose), 27.22g/L, nitrogen source (ammonium nitrate), 0.06g/L and manganese sulfate, 0.20g/L. The results show that pretreated straw consumes 2% less soda than that of unpretreated straw for cooking. The results of IR analysis show that it is oxidation of C. and -0-4 catalyzed by enzyme, which resulted in straw lignin bio-degraded.
The COD of E stage bleaching effluent that has been bio-treated for 5 days by P. chrysosprium is 71.8% less than that of unprocessed E stage bleaching waste-water and the color removes is 65%. This study is focused on the feasibility that utilize the sealed tube method to measure CODcr of bleaching effluent. The best wavelength to measure concentration of Cr6+ is 430nm. The results of /-test demonstrated that it can be used for measurement of CODcr same as the method described in Standard method, and the Pearson coefficient was 0.9995. The advantage of the sealed tube method is that the consumption of chemicals is less than standard method. The results of GC-MS detected by bio-treated and unprocessed E-stage wastewater show that the mainly pollution source of bleaching effluent is composed of macromolecule alkyl, alkene, phenyl ester and hydrin etc. These substances can be biodegraded completely
during fungi rapid growth stage by P. chrysosprium.
The mechanism of degradation of chlorophenol by P. chrysosporium with Volterra model was studied and the biodegrade dynamic formula was established to describe the biodegradation and effect of biosorption in batch-reactor. The results show that Volterra model describe the fungi growth process and the action of biosorption in the poison system well. By the experiment, 2,4-dichlorophenol can be eliminated above 99% by P. chrysosporium.
Finally, the Revised Feed forward Neural Network model of BP algorithmic was used to predict biodegradability of phenols substances using Molecule Connect Index to characterize structure of compounds. The correct rates are above 80% in predicting random sample. The artificial neural networks model is suitable for solving the problem of multivariate nonlinear function.
利用P. chrysosprium处理麦草原料，采用正交回归实验设计方法确定碳源(葡萄糖)、氮源(硝酸铵)、硫酸锰等对生物处理效果具有显著影响的因素水平最优化条件分别为(g/L)：27.22、0.06、0.20，作为麦草化学制浆预处理时间以6-12天为宜。根据最优化条件进行了碱法浆蒸煮试验，对比研究了生物预处理对麦草制浆的影响。结果表明：在用碱量8％--14％范围内，经过经过5天生物预处理的原料与未经处理的原料相比，在相同卡伯值和成纸强度条件下，用碱量降低2％。红外谱图分析结果显示，麦草木素生物降解是生物酶催化作用下的氧化反应，其结果C_α发生氧化、β-0-4键氧化裂解，导致木素大分子断裂溶出。
利用P. chrysosprium处理E段漂白废水(COD为389lmg/L)5天时COD去除率为71.8％、脱色率为65％。论文对密闭式分光光度法测定漂白废水COD方法进行了研究，确定最佳测定波长为430nm。t检验结果表明，该方法与标准COD_(cr)测定结果高度显著相关。实际应用中，该方法测定成本低，特别适宜批量试样同时测定，有利于消除系统误差。
对生物处理前后E段废水GC-MS检测结果证明，白腐菌P.chrysosprium处理漂白废水不产生二次污染；造成E段废水色度
的主要污染物为各种长碳链烷烃、烯烃以及苯基酯类、醇类化合
物，在菌体对数生长阶段可大量去除。
利用 olterra模型建立了 P Chwompum处理2，4一二氯酚模
拟废水动力学方程。通过动力学分析定量探讨了有毒化合物作用
下的菌体生长与底物降解关系。试验结果证实Volterra模型较好地
反映了有毒化合物对生物降解过程的抑制作用，较为精确地描述
了降解体系中菌体生长过程。动力学方程模拟结果与试验数据经
Pearson检验高度显著相关。动力学研究表明氯代酚的批次处理试
验中，生物吸附作用显著影响底物去除反应历程，这一特性在反
应器设计及氯代酚类有毒污染物生物降解机理研究中应给予重
视。在试验条件下使用P Chlysosporium处理2，4一二氯酚120h，
去除率＞99％。
在本文的最后部分，利用分子连接性指数（MCI）描述酚类化
合物结构，建立了人工神经网络（Artificial Neural Net，ANN）
模型预测酚类化合物可生物降解性能。使用BP算法对试验任选的
两组化合物的预测结果正确率均大于80％，预测结果显示了人工
神经元网络模型对于各种酚类化合物的可生物降解性具有良好的
预测能力，体现了网络模型自适应能力对于变量间相互关系和物
理意义不明的多变量非线性函数具有良好的逼近性能。
The research is focused on the application of white rot fungi in pulping and paper making field. In this paper, Phanerochaete chrysosprium (P. chrysosprium), a kind of representative white rot fungi, is used in straw bio-pretreatment and effluent bio-treatment respectively to decline the consumption of chemicals in pulp and the pollution of bleaching effluent.
The nutritional factors were optimized for better lignin bio-degradation with Orthogonal Regressive Analysis Method, i.e., carbon source (glucose), 27.22g/L, nitrogen source (ammonium nitrate), 0.06g/L and manganese sulfate, 0.20g/L. The results show that pretreated straw consumes 2% less soda than that of unpretreated straw for cooking. The results of IR analysis show that it is oxidation of C. and -0-4 catalyzed by enzyme, which resulted in straw lignin bio-degraded.
The COD of E stage bleaching effluent that has been bio-treated for 5 days by P. chrysosprium is 71.8% less than that of unprocessed E stage bleaching waste-water and the color removes is 65%. This study is focused on the feasibility that utilize the sealed tube method to measure CODcr of bleaching effluent. The best wavelength to measure concentration of Cr6+ is 430nm. The results of /-test demonstrated that it can be used for measurement of CODcr same as the method described in Standard method, and the Pearson coefficient was 0.9995. The advantage of the sealed tube method is that the consumption of chemicals is less than standard method. The results of GC-MS detected by bio-treated and unprocessed E-stage wastewater show that the mainly pollution source of bleaching effluent is composed of macromolecule alkyl, alkene, phenyl ester and hydrin etc. These substances can be biodegraded completely
during fungi rapid growth stage by P. chrysosprium.
The mechanism of degradation of chlorophenol by P. chrysosporium with Volterra model was studied and the biodegrade dynamic formula was established to describe the biodegradation and effect of biosorption in batch-reactor. The results show that Volterra model describe the fungi growth process and the action of biosorption in the poison system well. By the experiment, 2,4-dichlorophenol can be eliminated above 99% by P. chrysosporium.
Finally, the Revised Feed forward Neural Network model of BP algorithmic was used to predict biodegradability of phenols substances using Molecule Connect Index to characterize structure of compounds. The correct rates are above 80% in predicting random sample. The artificial neural networks model is suitable for solving the problem of multivariate nonlinear function.
引文
1 谌凡更，欧义芳．木质纤维原料的热化学液化．纤维素科学与技术[J]，2000，8 (1)：44-57．
2 J. M. Palmer and C. S. Evans, ISWPC-83, V. 3. E 19(1983)
3 R. Kondo, K. Hrazono, K. Sakai. Bleaching of Hardwood Kraft Pulp with Manganese Peroxidase Secreted from Phanerochaete sodida YK-624[J]. Applied and Environmental Microbiology, 1994, 60(12): 4359-4363.
4 K. Fujita, R. Kondo, K. Sakai, et al..Biobleaching of Softwood Kraft Pulp with White-rot Fungus IZU-154, Tappi J., 1993, 76(1): 81-84.
5 Die-sheng Tai, Minoru Terazawa, Chen-Loung Chen et al.. Lignin Biodegradation Products from Birch Wood by Phanerochaete chrysosporium[J], Holzforschung, 1990, 44(3): 185-190.
6 张厚民，A.M.Stomp,T.K.Kirk．生物技术与制浆造纸工业(上)[J]．中国造纸，1994(4)：51-57．
7 Ming Tien, T. Kent Kirk. Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds[J], Science, 1983, 221: 661-663.
8 J. K. Glenn, M. A. Morgan, M. B. Mayfield et al.. An Extracellular H_2O_-requiring Enzyme Preparation Involved in Lignin Biodegradation by the White Rot Basidiomycete Phanetochaete chrysosporium [J], Biochem Biophys res Commun, 1983, 114(3): 1077-1083.
9 Kuwahara M, et al. febs Lett, 1984; 169:247
10 R. L. Kelley, C. Adinarayana Reddy. Identification of Glucose Oxidase Activity as the Primary Source of Hydrogen Peroxide Production in Ligninolytic Cultures of Phanerochaete chrysosporium [J], Arch Microbiol, 1986, 144: 248-253.
11 P.J. Kersten, T. K. Kirk. Involvement of a New Enzyme, Glyoxal Oxidase, in Extracellular H_2O_2 Production by Phanerochaete chrysosporium[J], J. Bacterial, 1987 169(5): 2195-2201.
12 余惠生．白腐菌对稻草的生物降解规律及在生物制浆上的潜在应用[J]．中国造纸，1989(1)：16-20．
13 L. Otjen, R. A. Blanchette. Selective Delignification of Aspen Wood Blocks in Vitro by Three White Rot Basidiomycetes. Appl. Environ. Microbiol.1985, 50(3): 568-572.
14 余惠生，黄秀瑜．稻草木素生物降解的研究[J]．纤维素科学与技术，1(1)，1993：12-23．
15 A. Bjorkman. Studies on Finely Divided Wood, Part 1 [J]. Svensk Papperstidn, 59, 1956: 477-485.
16 Robert Bourbonnais, Michael G. Paice. Enzymatic Delignification of Kraft Pulp Using Laccase and a Mediator[J]. Tappi J., 1996, 79(6): 199-204.
17 L. A. Andersson, V. Renganathar, A. A. Chiu et al.. Spectral Characterization of Diarylpropane oxygenase, a Novel peroxide-dependent, lignin-degrading heme enzyme[J]. J. Biol. Chem, 1985, 2660(10):6080-6087
18 马登波，刘紫鹃，高培基等．木素生物降解的研究进展[J]．纤维素科学与技术[J]，4(1)，1996：1-12．
19 Glenn, J. K, M., Gold, M. H. 1985. Purification and characterization of an extracellular Mn(Ⅱ)-dependent peroxidase from the lignin-degrading basidiomycete Phanerochaete chrysosporium. Arch. Biochem. Biophys. 242:329-41
20 H. P. Call, I. Mucke, 1994 NCB-Conference, Proceedings
21 H.P.Call,I.Mucke,1995 INCBC.March,1995 In：王彩华 文湘辉 余惠生，国际生物漂白研究及应用的最新进展，广东造纸，3-4，1995：8-11，32
22 T. K. Kirk, H.-m. Chang. Decomposition of Lignin by White-rot fungi, Ⅰ. Isolation of Heavily Degraded Lignins from Decayed spruce[J]. Holzforschung 1974, 28(6): 217-222.
23 T.K. Kirk, H.-m. Chang. Decomposition of Lignin by White-rot Fungi. Ⅱ. Characterization of Heavily Degraded Lignins from Decayed Spruce. Holzforschung, 29(2), 1975: 56-64.
24 恽魁宏，高鸿宾，任贵忠．高等有机化学[M]，北京：高等教育出版社，1988,313-342
25 T K. Kirk, H.-m. Chang. Decomposition of Lignin by White-rot Fungi. Ⅱ. Characterization of Heavily Degraded Lignins from Decayed Spruce[J]. Holzforschung, 29(2), 1975: 56-64.
26 Enoki, A., G. P. goldsby and M. H. Gold. 1980. Metabolism of the lignin model compounds veratrylglycerol-β-guaiacyl ether by Phanerochaete chrysosporium. Arch. Microbiol. 125, 2327-232.
27 T K. Kirk, J. M. Harkin, E. B. Cowling. Degradation of the Lignin Model Compound Syringylglycol-β-guaiacyl Ether by Polyporus versicolor and Stereum Frustulatum [J], Biochim. Biophys. Acta., 1968, 165: 145-163.
28 Pew, J. C. and W. J. Connors. 1969. New structures from enzymatic dehydrogenation of lignin model p-hydroxypropiophenones. J. Org. Chem. 34,585-589
29 Yon P.-chr Ellwardt, K. Haider, L. Ernst. Untersuchung des mikrobiellen Ligninabaues durch ~(13)C-angereichertem DHP-Lignin aus Coniferylalkohol[J]. Holzforschung, 1981, 35(3): 103-109.
30 Barry K.Carpenter．有机反应机理测定的研究方法[M]．北京：北京大学出版社，1991，170-189
31 T. Reitberger, J. Gierer, Chemiluminescence as a Means to Study the Role of Hydroxyl Radicals in Oxidative Processes[J]. Holzforschung, 1988, 42(6): 351-356.
32 S. Backa, J. Gierer, K. Jansbo et al.. Hydroxyl Radicals in Oxidative Processes Studied by a New Chemiluminescence Method. Tappi Proceeding, 1999, 1-6.
33 Stefan Backa,,Josef Gierer, Torbjorn Reitberger et al.. Hydroxyl Radical Activity Associeted with the Growth of White-Rot fungi[J]. Holzforschung, 47(3), 1993: 181-187.
34 Taeko Hirano, Hiromi Tanaka, Akio Enoki. Relationship Between Production of Hydroxyl Radicals and Degradaton of Wood by the Brown-rot Fungus, Tyromyces palustris[J]. Holzforschung, 1997, 51 (5): 389-395
35 丁少军，王传槐，叶汉玲等．云芝(Coriolus versicolor)高木质素降解活性菌株筛选的研究[J]．纤维素科学与技术，1994，2(1)：32-37．
36 Kirk T. K., Takayoski Higuchi, chang Houmin, Lignin biodegradation microbiology, Chemistry and Potential Applications, Vol. 1, CrC Press, Inc. Boca Raton, Florida, 1980:203-312
37 Kirk T. K., Takayoski Higuchi, chang Houmin, Lignin biodegradation microbiology, Chemistry and Potential Applications, Vol. 1, CrC Press, Inc. Boca Raton, Florida, 1980:203-312
38 林鹿，陈嘉翔，余家鸾等．高效生产降解木素酶和降解聚木糖酶的菌种选育[J]．中国造纸学报，1996，11增刊：61-68．
39 Chen, C. L. and H. Chang, ACS/CSJ Chemical Congress, Abstract Cellulose, Paper and Textile Division, No. 132, Honolulu(1979).
40 Kirk, T K., Schultz, E., Conmnors, W. J., Lorenz, L. F., Zeikus, J. G. 1978. Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium, Arch. Microbiol. 117:277-85
41 M. S. A. Leisola, D. C. Ulmer, A. Fiechter. Factors affecting lignin degradation in lignocellulose by Phanerochaete chrysosporium [J], Arch. Microbiol. 137, 1983: 171-175.
42 B. D. Faison, T.K.Kirk. Relationship Between Lignin Degradation and Production of Reduced Oxygen Species by Phanerochaete chrysosporium[J]. Appl. Environ. Microbiol. 1983, 46(5): 1140-1145.
43 B. D Faison, T, K. Kirk. Factors Involved in the Regulation of a Ligninase Activity in Phanerochaete chrysosporium[J]. Appl. Eviron. Microbiol, 1985, 49(2): 299-304.
44 余惠生．白腐菌对稻草的生物降解规律及在生物制浆上的潜在应用[J]．中国造纸，1989，(1)：16-20．
45 Tadakazu Hiroi, Karl-Erik Eriksson. Microbiological Degradation of Lignin, Part 1. Influence of Cellulose on the Degradation of Lignins by the White-rot Rungus Pleurotus ostreatus[J]. Svensk Papperstidning 79(5), 1976: 157-161.
46 Tadakazu Hiroi, Karl-Erik Eriksson et al.. Microbiological Degradation of Lignin, Part 2. Influence of Cellulose upon the Degradation of Calcium Lignosulfonate of Various Molecular Sizes by the White-rot Fungus Pleurotus ostreatus[J], Svensk Papperstidning 79(5), 1976:162-166.
47 T. K. Kirk, W. J. Cormors, J. G. Zeikus, Recent Adv. Phytochem, Ⅱ,369(1977)In：木质素的化学(428)
48 Drew, S. W. and K. L. Kadam, Development in Industrial Microbiology, Vol. 20, 153(1979).
49 浦跃武，甄浩铭，冯书庭等．白腐菌产锰过氧化物酶条件的研究[J]．菌
务系统，1998，17(3)：251-255．
50 林鹿，邓耀杰，胡键等．富N条件下白腐菌对联苯的降解[J]，应用与环境生物学报，1999，5(3)：320-324．
51 李慧荣，陈建海．黄孢原毛平革菌对五氯酚生物降解研究[J]，江苏石油化工学院学报，1999，11(2)：24-28．
52 余惠生．白腐菌对稻草的生物降解规律及在生物制浆上的潜在应用[J]．中国造纸，1989，(1)：16-20．
53 T W. Jeferies, S. Choi, t. K. Kirk. Nutritional Regulation of Lignin Degradation by Phanerochaete chrysosporium[J], Applied and Environmental Microbiology, 1981, 42(2): 290-296.
54 I. D. Reid. Effects of Nitrogen Sources on Cellulose and Synthetic Lignin Degradation by Phanerochaete chrysosporium [J], Applied and Environmental Microbiology, 45(3), 1983: 838-842.
55 I. D. Reid, Effects of Nitrogen Supplements on Degradation of Aspen Wood Lignin and Carbohydrate Components by Phanerochaete chrysosporium[J], Applied and Environmental Microbiology, 1983, 45(3): 830-837.
56 Koichi Harazono, Ryuichiro Kondo, Kokki Sakai. Bleaching of hardwood kraft pulp with manganese peroxidase from Phanerochaete sordida YK-624 without addition of MnSO——4[J]. Applied and Environmental Microbiology, 1996,62(3): 913-917.
57 M. G. Paice, I. D. Reid, R. Bhourbonnais et al.. Manganese Peroxidase, Produced by Trametes versicolor During Pulp Bleaching, Demethylates and Delignifies Kraft Pulp, Applied and Environmental Microbiology, 1993, 59(1): 260-265.
58 Frederic H. Perie, Michael H. Gold. Manganese Regulation of Manganese Peroxidase Expression and Lignin Degradation by the White Rot Fungus Dichomitus Squalens[J], Applied and environmental Microbiology, 1991, 57(8): 2240-2245.
59 Frederick s. Archibald. Lignin Peroxidase Activity is not Important in Biological Bleaching and Delignification of Unbleached Kraft Pulp by Trametes versicolor[J]. Applied and Environmental Microblology, 1992, 58(9): 3101-3109
60 Frederick S. Archibald. A New Assay for Lignin-type Peroxidases Employing the Dye Azure B[J], Applied and Environmental Microbiology, 1992, 58(9): 3110-3116
61 邓耀杰，林鹿，詹怀宇．白腐菌处理亚硫酸盐浆CEH漂白废水及对氯代酚类纯底物的降解[J]，中国造纸学报，1999，14 增刊：63-67．
62 陈建海，李慧蓉．黄孢原毛平革菌对2，4-二氯苯酚的生物降解[J]，环境科学技术，1999，87(4)：26-28．
63 王双飞，陈嘉翔，余家鸾．吸附法固定白腐菌处理苇浆漂白废水的研究[J]，中国造纸，1995，14(6)：44-48．
64 程言君，纸浆漂白废水中有机氯化物的排放与控制[J]，中国造纸，1994，13 (4)：57-60
65 魏鹏月等．漂白废水中的氯化有机物及其处理[J]，中国造纸，1993，12(6)：59-65．
66 林鹿等．白腐菌对纸浆CEH漂白废水的脱色、消除毒性和芳香化合物的降解[J]，中国造纸学报，1996，11增刊：69-74．
67 林开荣等编．造纸工业环境保护[M]，中国轻工业出版社，1980．
68 陈嘉翔．清洁制浆技术[M]，北京：中国轻工业出版社，1996：1-5．
69 GB
70 D. Livernoche, L. Jurasek, M. Desrochers, J. orica, Removal of color from Kraft wastewaters with cultures of white-rot fungi and with immobilized mycelium ofcoriolus versicolor Biotechnol. Bioeng. 1983,25:2055-2065
71 Seon-Ho Lee, Hirofumi Hiral,Ryuichiro, et al. Screening of wood-rotting fungi for effluent decolorization of Kraft bleaching effluents, Seventh international symposium on wood and pulping chemistry, volume(3):432-437
72 Eriksson K-E L, et al. Microbiol and Enzymatic Degradation of Wood and Wood Components, Springer, New York, 1990,:249-318
73 Kirk T K, et al. Arch Microbiol, 1978,117:277
74 Thomas W. Jefffies, Suki Choi,Kent kirk, Nutritional regulation of degradation by Phanerchaete chrysosporium, Appl. Environ. Microbio. 1981, 42(2):290-296
75 B. D. Faison, T K. Kirk. Factors involved in the regulation of a ligninase activity in Phanerchaete chrysospodum, Appl. Environ. Microbic. 1985, 49(2):299-304
76 Frederick. C. Mickel, Jr., S. Balachandra Dass, et al. Role of manganese peroxidases and lignin peroxidases of Phanerochaete Chrysosporium in the decolorization of Kraft bleach plant effluent, Appl. Environ. Microbic. 1991,57(8):2368-2373
77 Zohar Kerem, Yitzhak Hadar, Effect of manganese on lignin degradation by Pleurotus ostreatus during solid-state fermentation, Appl. Environ. Microbiol., 1993,59(12):4115-4120
78 王伟，陈嘉翔．生物漂白的研究现状及应用前景，国际造纸 1994，13(6)：31-33
79 J. E. Adaskaveg, R. L. Gilbertson, M.R. Dunlap. Effects of incubation time and temperature on In Vitro selective delignification on silver leaf oak by Ganoderma colossum, Appl. Environ. Microbiol., 1995,61 (1): 138-144
80 Yue-zhong Li, Pei-ji Gao, Zu-nong Wang, Nutritional regulation of Ligninase, Celluases, and Xylanase synthesized in Phanerochate chrysosprium, Seventh international symposium on wood and pulping chemistry, volume(2):698-707
81 Marcel Asther, Cecile Capdevila, Georges Corrieu, Control of lignin
peroxidase by Phanerochaete chrysosporium INA-12 by temperature shifting, Appl. Environ. Microbio. 1988, 54(12) :3194-3196
82 Frank Tonon, Etienne Odier, Influence of veratryl alcohol and hydrogen peroxide on ligninase activity and ligninase production by Phanerochaete chrysosprium, Appl. Environ. Microbio. 1988,54(2):466-472
83 陈勇生，孙启俊，陈均等．重金属的生物吸附技术研究[J]，环境科学进展，1997，5(6)：34-43．
84 吴涓，李清彪，邓旭等．白腐真菌吸附铅的研究[J]，微生物学报，1999，39(1)：87-90．
85 陈勇生，闫姝，孙启俊等．酚类化合物的生物吸附特征与其结构的关系[J]，中国环境科学，1998，18(3)：248-251．
86 陈元彩，肖锦，詹怀宇．生物吸附作用对漂白废水中AOX去除作用的研究[J]．环境科学研究，1999，12(6)：28-31．
87 H. W. Liu, H. C. Lavallee, S. N. Lo. Mechanisms and Kinetics of Removal of Resin and Fatty Acids, Toxic Compounds, in CTMP Effluents During Aerobic Biological Treatment, Seventh international Symposium on Wood And Pulping Chemistry, Volume Two, Beijing, P. R. China, May 25-28,1993: 911-919.
88 沈东升，徐向阳，冯孝善．厌氧颗粒污泥对五氯苯酚的吸附、解吸和生物降解研究[J]，环境科学，1996，17(1)：20-23．
89
90 楼顺天等．基于MATLAB的系统分析与设计——神经网络[M]．西安：西安电子科技大学出版社，1998，9～14．
91 徐丽娜．神经网络控制[M]．哈尔滨：哈尔滨工业大学出版社，1999，1-3．
92 孙晞，鲁生业．利用神经网络对胺类有机物急性毒性的分类及定量预测[J]．环境科学，1998，19(1)：41-45．
93 张爱茜，魏东斌，王连生．用分子连接性指数研究氯代芳香族化合物对绿藻的毒性及QSA及分析[J]．环境化学，2000，19(3)：220-224．
94 王连生，韩朔睽．有机物定量结构-活性相关[M]．北京：中国环境科学出版社，1993，2-17．
95 陈景文，王帅杰，邹善伟等．不同量子化学算法在多环芳烃光解行为定量结构-性质关系(QSPR)中的应用[J]．中国环境科学，1999，19(4)．
96 李祚泳．B-P网络用于水质综合评价方法的研究[J]．环境工程，1995，13(2)：51-53．
97 刘国东等．水质综合评价的人工神经网络模型[J]．中国环境科学，1998，18(6)：514-517．
98 王世明等．利用人工神经网络预测芳香化合物的生物可降解性[J]．环境化学，2000，19(1)：48-52．
99 瞿福平，张晓健，何苗等．氯代芳香化合物生物降解性能与其化学结构的相关性．环境科学，1998，19(6)：26-28．
100 田禹等、BP及BPF人工神经网络对臭氧生物活性炭水处理系统建模的比较[J]，中国环境科学，1998，18(5)，In：http://chinainfo.gov.cn/index.htm.
101 Bhat, N., et. al. Use of neural nets for dynamic modeling and control of chemical process systems, Computers Chem. Engng. 14(4/5), 1990:573-583.
102 J. B. Rudd. Using a Neural Network System for Advanced Process Control[J]. Tappi J. 1991, 74(9): 153-159.
103 胡守仁等．神经网络导论[M]．长沙：国防科技大学出版社，1993，21-22．
104 Ikuo Momohara, et al. Oxidation of Dimethoxybenzene Derivatives by Lignm Peroxidase[C]. 10th ISWPC, Vol. Ⅲ. Yokohama Japan, 1999: 68-71.
105 Chen-Loung chen, et al. ~(13)C NMR Spectroscopic Study of Spruce Lignin Degraded by Phanerochaete chrysosporium, Ⅱ. Synthesis and Chemical Shifts of Model Compounds[J], Holzforschung, 1982, 36(5): 239-247.
106 李慧蓉．白腐真菌研究进展[J]．环境科学进展，1996．4(6)：69-77．
107 Sami sayadi, et al. Roles of Lignin Peroxidase and Manganese Peroxidase from Phanerochaete chrysosporium in the Decolorization of Olive Mill Wastewaters[J], Applied and Environmental Microbiology[J], 1995, 61(3): 1098-1103.
108 浦跃武等．白腐菌产锰过氧化物酶条件的研究[J]．菌物系统，1998，17(3)：251-255．
109 S. A. Medvedeva, et al.: Oxidative Destruction of Lignin and Phenol Compounds by Basidial Fungi Phanerochaete sanguinea and Coriolus villosus[C]. Seventh Inter national Symposium on Wood and Pulping Chemistry Proceedings, Vol. Two. Bejing: P. R. China. 1993, 650～658.
110 白新桂．数据分析与试验优化设计[M]．北京：清华大学出版社．
111 Kirk, T. K., Schultz, E., Conmnors, W. J., Lorenz, L. F., Zeikus, J. G. 1978. Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium, Arch. Microbiol. 117:277-85
112 李群等．木素降解菌Phanerochaete chrysosprium营养条件及其交互作用对选择性指数的影响[J]．天津轻工业学院学报，2000，(1)．
113 黄君礼．化学需氧量分析中的发展趋势[J]．环境科学丛刊，5(3)：26～34．
114 Best, D. E. and De Casseres, K.E., Wat. Pollut. Control, 77(1),138(1978).
115 陈元彩，等．密封式COD反应器快速测定法[J]．工业水处理．1996，16(3)：27-28．
116 李欣．哈尔滨师范大学学报，1995，(2)．
117 国家环保局编，水和废水检测分析方法[M]．北京：中国环境科学出版社，1989，第2版．
118 黄君礼，鲍治宇编著．紫外吸收光谱法及其应用[M]．北京：中国科学技术出版社，1992，265-270．
119 国家环保局编．水和废水检测分析方法[M]，第2版，北京：中国环境科学出版社，1989．
120 吴涓等．白腐真菌吸附铅的研究[J]．微生物学报．1999．39(1)：87～90．
121 李慧蓉．等 黄孢原毛平革菌对6种染料的脱色降解[J]，环境科学研究，1999，12(3)：14～17，21．
122 Die-sheng Tai et al., Lignin Biodegradation Products from Birch Wood Decayed by Phanerochaete chrysosporium. Part 2. The Constituents of Ether-soluble Low-molecular-weight Fracnons[J]. Holzforschung, 1990.44(4)57～262
123 F. S. Miranda et al.. ~(13)C NMR Spectroscopic Study of Spruce Lignin Degraded by Phanerochaete Chrysosporium, Ⅰ. New Structures[J]. Holzforschung, 1982, 36(4): 165-172.
124 Jean-Claude sigoillot et al. Enzymatic Treatment with Manganese Peroxidase from Phanerochaete chrysosporium for Enhancing Wheat Straw Pulp Characteristics [J]. Holzforschung, 1997, 51 (6): 549-556.
125 J.S. Yadav. R.E. Wallace. et al.. Mineralization of Mono- and Dichlorobenzenes and Simultaneous Degradation of Chloro- and Methyl-substituted Benzenes by the White Rot Fungus Phanerochaete chrysosporium [J], Applied and Environmental Microbiology, 1995, 61 (2): 667-680.
126 陈宗淇，戴闵光．胶体化学[M]．北京：高等教育出版社，1984，53-126．
127 孙红文，黄国兰，丛丽莉等．藻类对偶氮染料的降解及定量结构-生物降解性研究[J]，中国环境科学，1999，19(4)，In：http://chinainfo.gov.cn/indexs．htm.
128 楼顺天等．基于MATLAB的系统分析与设计——神经网络[M]．西安：西安电子科技大学出版社，1998，9～14．
129 瞿福平，张晓健，何苗等．氯苯类有机物生物降解性能及共代谢作用研究．中国环境科学，1997，17(2)：142～145
130 王连生．环境化学进展[M]．北京：化学工业出版社，1995，108-115．
131 陈勇生等．拓扑指数在定量结构生物降解性关系中的应用[J]．环境化学，1997，16(3)：208～214．
132 陈勇生等．32种芳香化合物的好氧生物降解性表征[J]．环境化学，1997，16(1)：43～48．
2 J. M. Palmer and C. S. Evans, ISWPC-83, V. 3. E 19(1983)
3 R. Kondo, K. Hrazono, K. Sakai. Bleaching of Hardwood Kraft Pulp with Manganese Peroxidase Secreted from Phanerochaete sodida YK-624[J]. Applied and Environmental Microbiology, 1994, 60(12): 4359-4363.
4 K. Fujita, R. Kondo, K. Sakai, et al..Biobleaching of Softwood Kraft Pulp with White-rot Fungus IZU-154, Tappi J., 1993, 76(1): 81-84.
5 Die-sheng Tai, Minoru Terazawa, Chen-Loung Chen et al.. Lignin Biodegradation Products from Birch Wood by Phanerochaete chrysosporium[J], Holzforschung, 1990, 44(3): 185-190.
6 张厚民，A.M.Stomp,T.K.Kirk．生物技术与制浆造纸工业(上)[J]．中国造纸，1994(4)：51-57．
7 Ming Tien, T. Kent Kirk. Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds[J], Science, 1983, 221: 661-663.
8 J. K. Glenn, M. A. Morgan, M. B. Mayfield et al.. An Extracellular H_2O_-requiring Enzyme Preparation Involved in Lignin Biodegradation by the White Rot Basidiomycete Phanetochaete chrysosporium [J], Biochem Biophys res Commun, 1983, 114(3): 1077-1083.
9 Kuwahara M, et al. febs Lett, 1984; 169:247
10 R. L. Kelley, C. Adinarayana Reddy. Identification of Glucose Oxidase Activity as the Primary Source of Hydrogen Peroxide Production in Ligninolytic Cultures of Phanerochaete chrysosporium [J], Arch Microbiol, 1986, 144: 248-253.
11 P.J. Kersten, T. K. Kirk. Involvement of a New Enzyme, Glyoxal Oxidase, in Extracellular H_2O_2 Production by Phanerochaete chrysosporium[J], J. Bacterial, 1987 169(5): 2195-2201.
12 余惠生．白腐菌对稻草的生物降解规律及在生物制浆上的潜在应用[J]．中国造纸，1989(1)：16-20．
13 L. Otjen, R. A. Blanchette. Selective Delignification of Aspen Wood Blocks in Vitro by Three White Rot Basidiomycetes. Appl. Environ. Microbiol.1985, 50(3): 568-572.
14 余惠生，黄秀瑜．稻草木素生物降解的研究[J]．纤维素科学与技术，1(1)，1993：12-23．
15 A. Bjorkman. Studies on Finely Divided Wood, Part 1 [J]. Svensk Papperstidn, 59, 1956: 477-485.
16 Robert Bourbonnais, Michael G. Paice. Enzymatic Delignification of Kraft Pulp Using Laccase and a Mediator[J]. Tappi J., 1996, 79(6): 199-204.
17 L. A. Andersson, V. Renganathar, A. A. Chiu et al.. Spectral Characterization of Diarylpropane oxygenase, a Novel peroxide-dependent, lignin-degrading heme enzyme[J]. J. Biol. Chem, 1985, 2660(10):6080-6087
18 马登波，刘紫鹃，高培基等．木素生物降解的研究进展[J]．纤维素科学与技术[J]，4(1)，1996：1-12．
19 Glenn, J. K, M., Gold, M. H. 1985. Purification and characterization of an extracellular Mn(Ⅱ)-dependent peroxidase from the lignin-degrading basidiomycete Phanerochaete chrysosporium. Arch. Biochem. Biophys. 242:329-41
20 H. P. Call, I. Mucke, 1994 NCB-Conference, Proceedings
21 H.P.Call,I.Mucke,1995 INCBC.March,1995 In：王彩华 文湘辉 余惠生，国际生物漂白研究及应用的最新进展，广东造纸，3-4，1995：8-11，32
22 T. K. Kirk, H.-m. Chang. Decomposition of Lignin by White-rot fungi, Ⅰ. Isolation of Heavily Degraded Lignins from Decayed spruce[J]. Holzforschung 1974, 28(6): 217-222.
23 T.K. Kirk, H.-m. Chang. Decomposition of Lignin by White-rot Fungi. Ⅱ. Characterization of Heavily Degraded Lignins from Decayed Spruce. Holzforschung, 29(2), 1975: 56-64.
24 恽魁宏，高鸿宾，任贵忠．高等有机化学[M]，北京：高等教育出版社，1988,313-342
25 T K. Kirk, H.-m. Chang. Decomposition of Lignin by White-rot Fungi. Ⅱ. Characterization of Heavily Degraded Lignins from Decayed Spruce[J]. Holzforschung, 29(2), 1975: 56-64.
26 Enoki, A., G. P. goldsby and M. H. Gold. 1980. Metabolism of the lignin model compounds veratrylglycerol-β-guaiacyl ether by Phanerochaete chrysosporium. Arch. Microbiol. 125, 2327-232.
27 T K. Kirk, J. M. Harkin, E. B. Cowling. Degradation of the Lignin Model Compound Syringylglycol-β-guaiacyl Ether by Polyporus versicolor and Stereum Frustulatum [J], Biochim. Biophys. Acta., 1968, 165: 145-163.
28 Pew, J. C. and W. J. Connors. 1969. New structures from enzymatic dehydrogenation of lignin model p-hydroxypropiophenones. J. Org. Chem. 34,585-589
29 Yon P.-chr Ellwardt, K. Haider, L. Ernst. Untersuchung des mikrobiellen Ligninabaues durch ~(13)C-angereichertem DHP-Lignin aus Coniferylalkohol[J]. Holzforschung, 1981, 35(3): 103-109.
30 Barry K.Carpenter．有机反应机理测定的研究方法[M]．北京：北京大学出版社，1991，170-189
31 T. Reitberger, J. Gierer, Chemiluminescence as a Means to Study the Role of Hydroxyl Radicals in Oxidative Processes[J]. Holzforschung, 1988, 42(6): 351-356.
32 S. Backa, J. Gierer, K. Jansbo et al.. Hydroxyl Radicals in Oxidative Processes Studied by a New Chemiluminescence Method. Tappi Proceeding, 1999, 1-6.
33 Stefan Backa,,Josef Gierer, Torbjorn Reitberger et al.. Hydroxyl Radical Activity Associeted with the Growth of White-Rot fungi[J]. Holzforschung, 47(3), 1993: 181-187.
34 Taeko Hirano, Hiromi Tanaka, Akio Enoki. Relationship Between Production of Hydroxyl Radicals and Degradaton of Wood by the Brown-rot Fungus, Tyromyces palustris[J]. Holzforschung, 1997, 51 (5): 389-395
35 丁少军，王传槐，叶汉玲等．云芝(Coriolus versicolor)高木质素降解活性菌株筛选的研究[J]．纤维素科学与技术，1994，2(1)：32-37．
36 Kirk T. K., Takayoski Higuchi, chang Houmin, Lignin biodegradation microbiology, Chemistry and Potential Applications, Vol. 1, CrC Press, Inc. Boca Raton, Florida, 1980:203-312
37 Kirk T. K., Takayoski Higuchi, chang Houmin, Lignin biodegradation microbiology, Chemistry and Potential Applications, Vol. 1, CrC Press, Inc. Boca Raton, Florida, 1980:203-312
38 林鹿，陈嘉翔，余家鸾等．高效生产降解木素酶和降解聚木糖酶的菌种选育[J]．中国造纸学报，1996，11增刊：61-68．
39 Chen, C. L. and H. Chang, ACS/CSJ Chemical Congress, Abstract Cellulose, Paper and Textile Division, No. 132, Honolulu(1979).
40 Kirk, T K., Schultz, E., Conmnors, W. J., Lorenz, L. F., Zeikus, J. G. 1978. Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium, Arch. Microbiol. 117:277-85
41 M. S. A. Leisola, D. C. Ulmer, A. Fiechter. Factors affecting lignin degradation in lignocellulose by Phanerochaete chrysosporium [J], Arch. Microbiol. 137, 1983: 171-175.
42 B. D. Faison, T.K.Kirk. Relationship Between Lignin Degradation and Production of Reduced Oxygen Species by Phanerochaete chrysosporium[J]. Appl. Environ. Microbiol. 1983, 46(5): 1140-1145.
43 B. D Faison, T, K. Kirk. Factors Involved in the Regulation of a Ligninase Activity in Phanerochaete chrysosporium[J]. Appl. Eviron. Microbiol, 1985, 49(2): 299-304.
44 余惠生．白腐菌对稻草的生物降解规律及在生物制浆上的潜在应用[J]．中国造纸，1989，(1)：16-20．
45 Tadakazu Hiroi, Karl-Erik Eriksson. Microbiological Degradation of Lignin, Part 1. Influence of Cellulose on the Degradation of Lignins by the White-rot Rungus Pleurotus ostreatus[J]. Svensk Papperstidning 79(5), 1976: 157-161.
46 Tadakazu Hiroi, Karl-Erik Eriksson et al.. Microbiological Degradation of Lignin, Part 2. Influence of Cellulose upon the Degradation of Calcium Lignosulfonate of Various Molecular Sizes by the White-rot Fungus Pleurotus ostreatus[J], Svensk Papperstidning 79(5), 1976:162-166.
47 T. K. Kirk, W. J. Cormors, J. G. Zeikus, Recent Adv. Phytochem, Ⅱ,369(1977)In：木质素的化学(428)
48 Drew, S. W. and K. L. Kadam, Development in Industrial Microbiology, Vol. 20, 153(1979).
49 浦跃武，甄浩铭，冯书庭等．白腐菌产锰过氧化物酶条件的研究[J]．菌
务系统，1998，17(3)：251-255．
50 林鹿，邓耀杰，胡键等．富N条件下白腐菌对联苯的降解[J]，应用与环境生物学报，1999，5(3)：320-324．
51 李慧荣，陈建海．黄孢原毛平革菌对五氯酚生物降解研究[J]，江苏石油化工学院学报，1999，11(2)：24-28．
52 余惠生．白腐菌对稻草的生物降解规律及在生物制浆上的潜在应用[J]．中国造纸，1989，(1)：16-20．
53 T W. Jeferies, S. Choi, t. K. Kirk. Nutritional Regulation of Lignin Degradation by Phanerochaete chrysosporium[J], Applied and Environmental Microbiology, 1981, 42(2): 290-296.
54 I. D. Reid. Effects of Nitrogen Sources on Cellulose and Synthetic Lignin Degradation by Phanerochaete chrysosporium [J], Applied and Environmental Microbiology, 45(3), 1983: 838-842.
55 I. D. Reid, Effects of Nitrogen Supplements on Degradation of Aspen Wood Lignin and Carbohydrate Components by Phanerochaete chrysosporium[J], Applied and Environmental Microbiology, 1983, 45(3): 830-837.
56 Koichi Harazono, Ryuichiro Kondo, Kokki Sakai. Bleaching of hardwood kraft pulp with manganese peroxidase from Phanerochaete sordida YK-624 without addition of MnSO——4[J]. Applied and Environmental Microbiology, 1996,62(3): 913-917.
57 M. G. Paice, I. D. Reid, R. Bhourbonnais et al.. Manganese Peroxidase, Produced by Trametes versicolor During Pulp Bleaching, Demethylates and Delignifies Kraft Pulp, Applied and Environmental Microbiology, 1993, 59(1): 260-265.
58 Frederic H. Perie, Michael H. Gold. Manganese Regulation of Manganese Peroxidase Expression and Lignin Degradation by the White Rot Fungus Dichomitus Squalens[J], Applied and environmental Microbiology, 1991, 57(8): 2240-2245.
59 Frederick s. Archibald. Lignin Peroxidase Activity is not Important in Biological Bleaching and Delignification of Unbleached Kraft Pulp by Trametes versicolor[J]. Applied and Environmental Microblology, 1992, 58(9): 3101-3109
60 Frederick S. Archibald. A New Assay for Lignin-type Peroxidases Employing the Dye Azure B[J], Applied and Environmental Microbiology, 1992, 58(9): 3110-3116
61 邓耀杰，林鹿，詹怀宇．白腐菌处理亚硫酸盐浆CEH漂白废水及对氯代酚类纯底物的降解[J]，中国造纸学报，1999，14 增刊：63-67．
62 陈建海，李慧蓉．黄孢原毛平革菌对2，4-二氯苯酚的生物降解[J]，环境科学技术，1999，87(4)：26-28．
63 王双飞，陈嘉翔，余家鸾．吸附法固定白腐菌处理苇浆漂白废水的研究[J]，中国造纸，1995，14(6)：44-48．
64 程言君，纸浆漂白废水中有机氯化物的排放与控制[J]，中国造纸，1994，13 (4)：57-60
65 魏鹏月等．漂白废水中的氯化有机物及其处理[J]，中国造纸，1993，12(6)：59-65．
66 林鹿等．白腐菌对纸浆CEH漂白废水的脱色、消除毒性和芳香化合物的降解[J]，中国造纸学报，1996，11增刊：69-74．
67 林开荣等编．造纸工业环境保护[M]，中国轻工业出版社，1980．
68 陈嘉翔．清洁制浆技术[M]，北京：中国轻工业出版社，1996：1-5．
69 GB
70 D. Livernoche, L. Jurasek, M. Desrochers, J. orica, Removal of color from Kraft wastewaters with cultures of white-rot fungi and with immobilized mycelium ofcoriolus versicolor Biotechnol. Bioeng. 1983,25:2055-2065
71 Seon-Ho Lee, Hirofumi Hiral,Ryuichiro, et al. Screening of wood-rotting fungi for effluent decolorization of Kraft bleaching effluents, Seventh international symposium on wood and pulping chemistry, volume(3):432-437
72 Eriksson K-E L, et al. Microbiol and Enzymatic Degradation of Wood and Wood Components, Springer, New York, 1990,:249-318
73 Kirk T K, et al. Arch Microbiol, 1978,117:277
74 Thomas W. Jefffies, Suki Choi,Kent kirk, Nutritional regulation of degradation by Phanerchaete chrysosporium, Appl. Environ. Microbio. 1981, 42(2):290-296
75 B. D. Faison, T K. Kirk. Factors involved in the regulation of a ligninase activity in Phanerchaete chrysospodum, Appl. Environ. Microbic. 1985, 49(2):299-304
76 Frederick. C. Mickel, Jr., S. Balachandra Dass, et al. Role of manganese peroxidases and lignin peroxidases of Phanerochaete Chrysosporium in the decolorization of Kraft bleach plant effluent, Appl. Environ. Microbic. 1991,57(8):2368-2373
77 Zohar Kerem, Yitzhak Hadar, Effect of manganese on lignin degradation by Pleurotus ostreatus during solid-state fermentation, Appl. Environ. Microbiol., 1993,59(12):4115-4120
78 王伟，陈嘉翔．生物漂白的研究现状及应用前景，国际造纸 1994，13(6)：31-33
79 J. E. Adaskaveg, R. L. Gilbertson, M.R. Dunlap. Effects of incubation time and temperature on In Vitro selective delignification on silver leaf oak by Ganoderma colossum, Appl. Environ. Microbiol., 1995,61 (1): 138-144
80 Yue-zhong Li, Pei-ji Gao, Zu-nong Wang, Nutritional regulation of Ligninase, Celluases, and Xylanase synthesized in Phanerochate chrysosprium, Seventh international symposium on wood and pulping chemistry, volume(2):698-707
81 Marcel Asther, Cecile Capdevila, Georges Corrieu, Control of lignin
peroxidase by Phanerochaete chrysosporium INA-12 by temperature shifting, Appl. Environ. Microbio. 1988, 54(12) :3194-3196
82 Frank Tonon, Etienne Odier, Influence of veratryl alcohol and hydrogen peroxide on ligninase activity and ligninase production by Phanerochaete chrysosprium, Appl. Environ. Microbio. 1988,54(2):466-472
83 陈勇生，孙启俊，陈均等．重金属的生物吸附技术研究[J]，环境科学进展，1997，5(6)：34-43．
84 吴涓，李清彪，邓旭等．白腐真菌吸附铅的研究[J]，微生物学报，1999，39(1)：87-90．
85 陈勇生，闫姝，孙启俊等．酚类化合物的生物吸附特征与其结构的关系[J]，中国环境科学，1998，18(3)：248-251．
86 陈元彩，肖锦，詹怀宇．生物吸附作用对漂白废水中AOX去除作用的研究[J]．环境科学研究，1999，12(6)：28-31．
87 H. W. Liu, H. C. Lavallee, S. N. Lo. Mechanisms and Kinetics of Removal of Resin and Fatty Acids, Toxic Compounds, in CTMP Effluents During Aerobic Biological Treatment, Seventh international Symposium on Wood And Pulping Chemistry, Volume Two, Beijing, P. R. China, May 25-28,1993: 911-919.
88 沈东升，徐向阳，冯孝善．厌氧颗粒污泥对五氯苯酚的吸附、解吸和生物降解研究[J]，环境科学，1996，17(1)：20-23．
89
90 楼顺天等．基于MATLAB的系统分析与设计——神经网络[M]．西安：西安电子科技大学出版社，1998，9～14．
91 徐丽娜．神经网络控制[M]．哈尔滨：哈尔滨工业大学出版社，1999，1-3．
92 孙晞，鲁生业．利用神经网络对胺类有机物急性毒性的分类及定量预测[J]．环境科学，1998，19(1)：41-45．
93 张爱茜，魏东斌，王连生．用分子连接性指数研究氯代芳香族化合物对绿藻的毒性及QSA及分析[J]．环境化学，2000，19(3)：220-224．
94 王连生，韩朔睽．有机物定量结构-活性相关[M]．北京：中国环境科学出版社，1993，2-17．
95 陈景文，王帅杰，邹善伟等．不同量子化学算法在多环芳烃光解行为定量结构-性质关系(QSPR)中的应用[J]．中国环境科学，1999，19(4)．
96 李祚泳．B-P网络用于水质综合评价方法的研究[J]．环境工程，1995，13(2)：51-53．
97 刘国东等．水质综合评价的人工神经网络模型[J]．中国环境科学，1998，18(6)：514-517．
98 王世明等．利用人工神经网络预测芳香化合物的生物可降解性[J]．环境化学，2000，19(1)：48-52．
99 瞿福平，张晓健，何苗等．氯代芳香化合物生物降解性能与其化学结构的相关性．环境科学，1998，19(6)：26-28．
100 田禹等、BP及BPF人工神经网络对臭氧生物活性炭水处理系统建模的比较[J]，中国环境科学，1998，18(5)，In：http://chinainfo.gov.cn/index.htm.
101 Bhat, N., et. al. Use of neural nets for dynamic modeling and control of chemical process systems, Computers Chem. Engng. 14(4/5), 1990:573-583.
102 J. B. Rudd. Using a Neural Network System for Advanced Process Control[J]. Tappi J. 1991, 74(9): 153-159.
103 胡守仁等．神经网络导论[M]．长沙：国防科技大学出版社，1993，21-22．
104 Ikuo Momohara, et al. Oxidation of Dimethoxybenzene Derivatives by Lignm Peroxidase[C]. 10th ISWPC, Vol. Ⅲ. Yokohama Japan, 1999: 68-71.
105 Chen-Loung chen, et al. ~(13)C NMR Spectroscopic Study of Spruce Lignin Degraded by Phanerochaete chrysosporium, Ⅱ. Synthesis and Chemical Shifts of Model Compounds[J], Holzforschung, 1982, 36(5): 239-247.
106 李慧蓉．白腐真菌研究进展[J]．环境科学进展，1996．4(6)：69-77．
107 Sami sayadi, et al. Roles of Lignin Peroxidase and Manganese Peroxidase from Phanerochaete chrysosporium in the Decolorization of Olive Mill Wastewaters[J], Applied and Environmental Microbiology[J], 1995, 61(3): 1098-1103.
108 浦跃武等．白腐菌产锰过氧化物酶条件的研究[J]．菌物系统，1998，17(3)：251-255．
109 S. A. Medvedeva, et al.: Oxidative Destruction of Lignin and Phenol Compounds by Basidial Fungi Phanerochaete sanguinea and Coriolus villosus[C]. Seventh Inter national Symposium on Wood and Pulping Chemistry Proceedings, Vol. Two. Bejing: P. R. China. 1993, 650～658.
110 白新桂．数据分析与试验优化设计[M]．北京：清华大学出版社．
111 Kirk, T. K., Schultz, E., Conmnors, W. J., Lorenz, L. F., Zeikus, J. G. 1978. Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium, Arch. Microbiol. 117:277-85
112 李群等．木素降解菌Phanerochaete chrysosprium营养条件及其交互作用对选择性指数的影响[J]．天津轻工业学院学报，2000，(1)．
113 黄君礼．化学需氧量分析中的发展趋势[J]．环境科学丛刊，5(3)：26～34．
114 Best, D. E. and De Casseres, K.E., Wat. Pollut. Control, 77(1),138(1978).
115 陈元彩，等．密封式COD反应器快速测定法[J]．工业水处理．1996，16(3)：27-28．
116 李欣．哈尔滨师范大学学报，1995，(2)．
117 国家环保局编，水和废水检测分析方法[M]．北京：中国环境科学出版社，1989，第2版．
118 黄君礼，鲍治宇编著．紫外吸收光谱法及其应用[M]．北京：中国科学技术出版社，1992，265-270．
119 国家环保局编．水和废水检测分析方法[M]，第2版，北京：中国环境科学出版社，1989．
120 吴涓等．白腐真菌吸附铅的研究[J]．微生物学报．1999．39(1)：87～90．
121 李慧蓉．等 黄孢原毛平革菌对6种染料的脱色降解[J]，环境科学研究，1999，12(3)：14～17，21．
122 Die-sheng Tai et al., Lignin Biodegradation Products from Birch Wood Decayed by Phanerochaete chrysosporium. Part 2. The Constituents of Ether-soluble Low-molecular-weight Fracnons[J]. Holzforschung, 1990.44(4)57～262
123 F. S. Miranda et al.. ~(13)C NMR Spectroscopic Study of Spruce Lignin Degraded by Phanerochaete Chrysosporium, Ⅰ. New Structures[J]. Holzforschung, 1982, 36(4): 165-172.
124 Jean-Claude sigoillot et al. Enzymatic Treatment with Manganese Peroxidase from Phanerochaete chrysosporium for Enhancing Wheat Straw Pulp Characteristics [J]. Holzforschung, 1997, 51 (6): 549-556.
125 J.S. Yadav. R.E. Wallace. et al.. Mineralization of Mono- and Dichlorobenzenes and Simultaneous Degradation of Chloro- and Methyl-substituted Benzenes by the White Rot Fungus Phanerochaete chrysosporium [J], Applied and Environmental Microbiology, 1995, 61 (2): 667-680.
126 陈宗淇，戴闵光．胶体化学[M]．北京：高等教育出版社，1984，53-126．
127 孙红文，黄国兰，丛丽莉等．藻类对偶氮染料的降解及定量结构-生物降解性研究[J]，中国环境科学，1999，19(4)，In：http://chinainfo.gov.cn/indexs．htm.
128 楼顺天等．基于MATLAB的系统分析与设计——神经网络[M]．西安：西安电子科技大学出版社，1998，9～14．
129 瞿福平，张晓健，何苗等．氯苯类有机物生物降解性能及共代谢作用研究．中国环境科学，1997，17(2)：142～145
130 王连生．环境化学进展[M]．北京：化学工业出版社，1995，108-115．
131 陈勇生等．拓扑指数在定量结构生物降解性关系中的应用[J]．环境化学，1997，16(3)：208～214．
132 陈勇生等．32种芳香化合物的好氧生物降解性表征[J]．环境化学，1997，16(1)：43～48．