嗜鞣管囊酵母P-01发酵玉米秸秆糖化液关键技术研究
|
摘要
本文对利用玉米秸秆生产纤维燃料乙醇的关键技术进行了研究,包括秸秆糖化液中有毒物质脱除和提高酵母菌发酵性能两个主要方面,以提高发酵的结果,为纤维燃料乙醇的产业化奠定基础。
对糖化液进行了分析表明,糖成分主要有葡萄糖、木糖、甘露糖、半乳糖、阿拉伯糖等;D-葡萄糖和木糖的比例约为1.22,二者占总还原糖76.18%左右;糖化液中乙酸含量为4.68g/L,糠醛含量为0.20g/L。
嗜鞣管囊酵母P-01单独发酵木糖的糖醇转化率为15.23%;P-01发酵纯糖的糖醇转化率为28.54%;P-01发酵玉米秸秆糖化液的糖醇转化率为17.36%,在玉米秸秆糖化液中乙酸是酵母菌发酵的主要抑制物质,而糠醛对酵母菌无显著影响。
对糖化液的脱毒试验结果表明,通过提高发酵培养基中的pH(由5.0升至5.5),P-01的糖醇转化率可从20.61%提高到23.58%;用熟石灰对糖化液脱毒处理,P-01的糖醇转化率可从18.90%提高到21.57%;蒸煮糖化液脱毒,P-01的糖醇转化率由24.12%提高到25.25%;糖化液中0.1g/L的乙醛能使P-01的糖醇转化率从23.06%,提高到24.00%,0.1g/L的沸石可使P-01的糖醇转化率从16.95%提高到18.94%,1g/L的活性炭可使P-01的糖醇转化率从16.95%提高到18.39%。
在提高酵母发酵性能方面,在酶解时用磷酸缓冲液,更适合P-01发酵玉米秸秆糖化液;适合P-01发酵玉米秸秆糖化液的培养基配方为:酵母膏0.03 g/100ml,蛋白胨0.05 g/100ml,尿素0.02 g/100ml,磷酸氢二铵0.01 g/100ml,吐温80 25μm/100ml,聚乙烯醇2.5mg/100ml,L-谷氨酰胺62.5mg/100ml,pH5.0;添加油酸对酵母菌发酵结果的影响不明显;在36h时补料,发酵结束的乙醇含量为2.05%(v/v)比对照的乙醇含量1.54%(v/v)高出0.51百分点,P-01的糖醇转化率为28.23%,略低于对照的28.71%。
In this paper,the key technology of fuel ethanol produced from corn stalk material by Pachysolen tannophilus P-01 was studied.Including two main aspects,one was the detoxification of corn stalk saccharified liquid and another was enhancing fermentation of the yeast,improving the results,and putting the way for fuel ethanol industry.
As analyzing the saccharified liquid,the main sugar was glucose,xylose,mannose, galactose,arabinose et al.;the ratio of D-glucose to xylose was about 1.22,D-glucose and xylose is accounting for about 76.18%of total sugar;the acetic acid and furfural at concentrations of 4.68g/L and 0.20g/L,respecttively.
Fermentation of xylose by P-01,and the ethanol conversion rate was 15.23%; co-fermentation of glucose and xylose P-01,and the ethanol conversion rate was 28.54%;and fermertation of saccharified liquid by P-01,and the ethanol conversion rate was 17.36%.In the saccharified liquid,P-01 was inhibited by acetic acid and not by furfural.
The results of detoxification to the saccharified liquid show that,adjusting pH from 5.0 to 5.5 to the saccharified liquid,the ethanol conversion rate of P-01 increased from 20.61%to 23.58%;treatment to the saccharified liquid by hydrated lime,the ethanol conversion rate of P-01 increased from 18.90%to 21.57%;detoxification by cooking to the saccharified liquid,the ethanol conversion rate of P-01 increased from 24.12%to 25.25%;addition 0.1g/L acetaldehyde,0.1g/L zeolite,1g/L activated carbon can increase The ethanol conversion rate from 23.06%to 24.00%,from 16.95%to 18.94%,from 16.95%to 18.39%,respecttiveiy.
For improving fermentation of P-01,choose the phosphoric acid buffer is more suitable for co-fermentation to fuel ethanol production from corn stalk;the medium formulation which is suitable for fermentation of saccharified liquid by P-01 is yeast extract 0.03 g/100ml,peptone 0.05 g/100ml,urea 0.02 g/100ml,(NH4)_2PO_4 0.01 g/100ml,tween 80 25μm/100ml,PVA 2.5mg/100ml,L-glutamine 62.5mg/100ml, pH5.0.The impact of oleic acid on fermentation is weak;when 36h feeding batch culture,at the end of fermentation the ethanol concentration is 2.05(v/v),0.51 higher than the ethanol concentration of control,the ethanol conversion rate of P-01 was 28.23%,slightly lower than 28.71%of the control.
对糖化液进行了分析表明,糖成分主要有葡萄糖、木糖、甘露糖、半乳糖、阿拉伯糖等;D-葡萄糖和木糖的比例约为1.22,二者占总还原糖76.18%左右;糖化液中乙酸含量为4.68g/L,糠醛含量为0.20g/L。
嗜鞣管囊酵母P-01单独发酵木糖的糖醇转化率为15.23%;P-01发酵纯糖的糖醇转化率为28.54%;P-01发酵玉米秸秆糖化液的糖醇转化率为17.36%,在玉米秸秆糖化液中乙酸是酵母菌发酵的主要抑制物质,而糠醛对酵母菌无显著影响。
对糖化液的脱毒试验结果表明,通过提高发酵培养基中的pH(由5.0升至5.5),P-01的糖醇转化率可从20.61%提高到23.58%;用熟石灰对糖化液脱毒处理,P-01的糖醇转化率可从18.90%提高到21.57%;蒸煮糖化液脱毒,P-01的糖醇转化率由24.12%提高到25.25%;糖化液中0.1g/L的乙醛能使P-01的糖醇转化率从23.06%,提高到24.00%,0.1g/L的沸石可使P-01的糖醇转化率从16.95%提高到18.94%,1g/L的活性炭可使P-01的糖醇转化率从16.95%提高到18.39%。
在提高酵母发酵性能方面,在酶解时用磷酸缓冲液,更适合P-01发酵玉米秸秆糖化液;适合P-01发酵玉米秸秆糖化液的培养基配方为:酵母膏0.03 g/100ml,蛋白胨0.05 g/100ml,尿素0.02 g/100ml,磷酸氢二铵0.01 g/100ml,吐温80 25μm/100ml,聚乙烯醇2.5mg/100ml,L-谷氨酰胺62.5mg/100ml,pH5.0;添加油酸对酵母菌发酵结果的影响不明显;在36h时补料,发酵结束的乙醇含量为2.05%(v/v)比对照的乙醇含量1.54%(v/v)高出0.51百分点,P-01的糖醇转化率为28.23%,略低于对照的28.71%。
In this paper,the key technology of fuel ethanol produced from corn stalk material by Pachysolen tannophilus P-01 was studied.Including two main aspects,one was the detoxification of corn stalk saccharified liquid and another was enhancing fermentation of the yeast,improving the results,and putting the way for fuel ethanol industry.
As analyzing the saccharified liquid,the main sugar was glucose,xylose,mannose, galactose,arabinose et al.;the ratio of D-glucose to xylose was about 1.22,D-glucose and xylose is accounting for about 76.18%of total sugar;the acetic acid and furfural at concentrations of 4.68g/L and 0.20g/L,respecttively.
Fermentation of xylose by P-01,and the ethanol conversion rate was 15.23%; co-fermentation of glucose and xylose P-01,and the ethanol conversion rate was 28.54%;and fermertation of saccharified liquid by P-01,and the ethanol conversion rate was 17.36%.In the saccharified liquid,P-01 was inhibited by acetic acid and not by furfural.
The results of detoxification to the saccharified liquid show that,adjusting pH from 5.0 to 5.5 to the saccharified liquid,the ethanol conversion rate of P-01 increased from 20.61%to 23.58%;treatment to the saccharified liquid by hydrated lime,the ethanol conversion rate of P-01 increased from 18.90%to 21.57%;detoxification by cooking to the saccharified liquid,the ethanol conversion rate of P-01 increased from 24.12%to 25.25%;addition 0.1g/L acetaldehyde,0.1g/L zeolite,1g/L activated carbon can increase The ethanol conversion rate from 23.06%to 24.00%,from 16.95%to 18.94%,from 16.95%to 18.39%,respecttiveiy.
For improving fermentation of P-01,choose the phosphoric acid buffer is more suitable for co-fermentation to fuel ethanol production from corn stalk;the medium formulation which is suitable for fermentation of saccharified liquid by P-01 is yeast extract 0.03 g/100ml,peptone 0.05 g/100ml,urea 0.02 g/100ml,(NH4)_2PO_4 0.01 g/100ml,tween 80 25μm/100ml,PVA 2.5mg/100ml,L-glutamine 62.5mg/100ml, pH5.0.The impact of oleic acid on fermentation is weak;when 36h feeding batch culture,at the end of fermentation the ethanol concentration is 2.05(v/v),0.51 higher than the ethanol concentration of control,the ethanol conversion rate of P-01 was 28.23%,slightly lower than 28.71%of the control.
引文
[1]戴林,李景明.中国生物质能转化技术发展与评价[M].北京:中国环境科学出版社,1998
[2]张雷.中国能源供应的调整.中国能源,1999,3:12-14
[3]闫长乐.中国能源发展报告[M].北京:经济管理出版社,1994
[4]刘卿,胡迎春.21世纪初我国石油安全战略选择.国土资源科技管理,2002,6:25-30
[5]郭云涛.国家能源安全及能源管理体制面临的挑战及对策.中国煤炭,2002,28(9):4-8
[6]郭祥冰.世界可再生能源发展状况和我国发展目标.福建能源开发与节约,2001,3:6-9
[7]L.Nicole,X.Youkun.Production of ethanol from recycled paper sludge using cellulose and yest Kluverichia Coli.Biotechnology Progress,1997,12(2):135-143
[8]傅其军.燃料乙醇的发展前景.广西轻工业,2001,2:6-10
[9]M.Jose,R.Goldemberg Jose.Alcohol program Energy Policy,1999,27(4):229-245
[10]余燕春.利用农业废弃物生产酒精的社会效益[J].江西林业科,1996,6:18-20
[11]黄治玲.燃料乙醇工业的全球发展概况[J].化工管理,2002,3:34-35
[12]刘全根.乙醇汽油的应用[J]炼油设计,2001,32(2):40-45
[13]曲音波,高培基.造纸厂废物发酵生产纤维素酶、酒精和酵母综合工艺的研究进展[J].食品与发酵工业,1993,19(6):62-65.
[14]Nick Nagie.A Process Economic to Develop a Dilute-Acid Cellulose Hydrolysis process to produce ethanol from Biomass[J].Appl.Biotechnol.,1999,77-79:599-607.
[15]Jeewon Lee..Biological conversion of lignocellosic biomass to ethanol [J].J.Biotechnol.,1997,56:1-24
[16]孙玉英,谭海刚.发酵木糖酵母菌株的选育.广州食品工业科技,2003,18(4):1-4
[17]Gregg D.,Saddler J.N..Bioconversion of lignocellulosi-biomass to ethanol.Biomass and Bioenergy,1995,9(1-5):287-302
[18]H.Krrishna,K.Prasanthi.Simultaneous saccharification and fermentation of pretreated sugar cane leaves to ethanol[J].Process Biochemistry,2001,(8):825-830
[19]曲音波.开展生物制转化酒精研究实现液体燃料可持续供应 济南:中国资源生物技术与糖工程学术研讨会论文集
[20]吴坤等.木质素生物降解进展.河南农业大学学报,2000,34(4):349-354
[21]鲍晓明.嗜热细菌木糖异构酶基因XYLA在酿酒酵母中的高效表达.微生物学报,1999,39(1):49-54
[22]宋安东.生物质(秸秆)纤维燃料乙醇生产工艺试验研究[D].郑州:河南农业大学,2003
[23]沈翌等.酿酒酵母工业菌株中Ⅺ木糖代谢途径的建立.中国生物工程杂志,2005,25(9):69-73
[24]鲍晓明.酒精生产酵母菌株木糖代谢工程及其发酵工艺.济南:中国资源生物技术与糖工程学术研讨会论文集.2005
[25]勇强等.玉米秸秆生物转化制取酒精的中间试验.济南:中国资源生物技术与糖工程学术研讨会论文集.2005
[26]邓良伟.纤维素类物质生产燃料酒精研究进展.食品与发酵工业,1995,6:69-72
[27]黄忠乾,龙章富等.农作物秸秆资源的综合利用[J].资源开发与市场,2002(2):32-34
[28]陈艳萍等.戊糖发酵微生物及选育[J].纤维素科学与技术,2001,9(3):57-61
[29]Wang P Y,Shopsis C,Schneider H.Biochem Biophys Res Commun,1980,94:248-254
[30]马文漪,杨柳燕,程树培.废物乙醇化处理.环境科学进展,1994,(2)3:58-63
[31]孙可伟.固体废弃物资源化的现状和展望[J].中国资源综合利用,20(1):10-13
[32]张继泉等.预处理玉米秸杆生产燃料酒精的研究.广州食品工业科技,2002,18(3):1-3
[33]张书祥,肖亚中等.添加营养盐对酒精酵母发酵的影响.生物学杂志1997,114(1):23-25
[34]Honwy,Stcvis P,roscnfcld S et al,Biotechnol bioeng symp,1983,13:245-255.
[35]Lawford,Rousseau.Improving fermentation performance of Recombinant Zymomonas in acetic acid-containing media.Appl.Biochem.Biotechnol.1998,70-72,161-172
[36]Gong C.S.,GT.Conversion of D-xylose to ethanol by yeast.Eidited by Duck worgh H.E.and Thompson,E.A.,1983,525-555
[37]Shipra D.,Mita B..Production of alcohol fron starch by immobilized cell of saccharomyces diasrtaricus in batch and xomtinuous process.Process Biochemistry,1991,25(2):43-45
[38]Jeffrices T.W.Emerging technology for fermenting D-xylose.Trends in Biotechnology,1985,3(8):208-211
[39]Mohammad J.Taherzadeh,Claes Niklasson et al.Conversion of dilute-acid hydrolyzates of spruce and birch to ethanol by fed-batch fermentation.Bioresource technology[J],1999,69:59-66
[40]You K.M.,Rosenfield C.L.,Knipple D.C..Ethanol tolerance in the yeast Saccharomyces cerevisiae is dependent on cellular oleic acid content.Appl Environ Microbiol,2003,69(3):1499-1503
[41]沈楠,肖冬光.大豆脂肪酸对酿酒酵母酒精耐性的影响.酿酒科技,2006,11:17-20
[42]A.R.Barber,H.Hansson.Acetaldehyde stimulation of the growth of Saccharomyces cerevisiae in the presence of inhibitors found in lignocellulose-to-ethanol fermentations,Journal of Industrial Microbiology,2000,20,104-108
[43]G.A.Stanley,N.G.Douglas,E.J.Every,T.Tzanatosand N.B.Pamment.BIOTECHNOLOGY LETTERSVolume,1993,15(12):1199-1204
[44]Taherzadeh M.J.,Niklasson C.,Liden G..On-line control of fed-batch fermentation of dilute-acid hydrolyzates.Biotechnol Bioeng,2000,69:330-338
[45]Olsson L.,Hahn-HEagerdal B..Fermentation of lignocellulosic hydrolysates for ethanol production.Enzyme Microb.Technol.,1996,18:312-331
[46]Parajro J.C.,Domringuez H..Charcoal adsorption of wood hydrolysates for improving their fementability:Influence of the operational conditions.Bioresource Technol.,1996,57:179-185
[47]Palmqvist E.,Hahn-HEagerdal B..Fermentation of lignocellulosic hydrolysates.Ⅱ:Inhibitors and mechanisms of inhibition.Bioresource Technol.,2000,74:25-33
[48]Roberto I.C..Utilization of sugarcane bagasse hemicellulosic hydrolysate by Pichia stipitis for the production of ethanol.Process Biochem.,1991,26:15-21
[49]Delgenes J..Effects of lignocellulose degradation products on ethanol fermentations of glucose and xylose by Saccharomyces cerevisiae,Zymomonas mobilis,Pichia stipitis and Candida shehatae.Enzyme Microb.Technol.,1996,19:220-225
[50]Nigam J.N..Ethanol production from wheat straw hemicellulose hydrolysate by Pichia stipitis.J.Biotechnol.,2001,87:17-27
[51]Alves L.A..Pretreatment of cane bagasse hemicellulose hydrolyzate for xylitol production by Candida guilliermondii.Appl.Biochem.Biotechnol.,1998,70-72,89-98
[52]Martinez A..Effects of Ca(OH)_2 treatments on the composition and toxicity of bagasse hemicellulose hydrolysates.Biotechnol.Bioeng.,2000,69:526-536
[53]Parajro J.C..Biotechnological production of xylitol Part 3:Operation in culture media made from lignocellulose hydrolysates.Bioresource Technol.,1998,66:25-40
[54]Villa P..Influence of phenolic compounds on the bioprocess of xylitol production by Candida guilliermondii.In:Esbes-2 European Symposium On Biochemical Engineering Science,2.Porto-Portugal,1998
[55]McMillan J.D..Pretreatment of lignocellulosic biomass.In:Himmel M.E.,Baker J.O.,Overend R.P.(Eds.).Conversion of Hemicellulose Hydrolyzates to Ethanol.American Chemical Society Symposium,Washington DC,1994:292-324
[56]Van Zyl C.,Prior B.A.,du Preez J.C..Acetic acid inhibition of D-xylose fermentation by Pichia stipitis.Enzyme Microb.Technol.,1991,13:82-86
[57]Felipe M.G.A..Veira M.V..Effect of acetic acid on xylose fermentation to xylitol by Candida guilliermondii[J].Basic Microb.,1995,35:171-177
[58]Palmqvist E..Main and interaction effects of acetic acid,furfural and phydroxybenzoic acid on growth and ethanol productivity of yeasts.Biotechnol. Bioeng.,1999,63:46-55
[59]Watson N.E..Factors an acid treated bagasse inhibiting ethanol production from Dxylose by Pachysolen tannophilus.Enzyme Microb.Technol.,1984,6:451-456
[60]Roberto I.C..Utilization of cane bagasse hemicellulosic hydrolysate by Candida guilliermondii for xylitol production.Bioresource Technol.,1991,36:271-275
[61]Kuhad R.C.,Singh A..Lignocellulose biotechnology:Current and future prospects.Crit.Rev.Biotechnol.,1993,13:151-172
[62]Winkelhausen E.,guzmanova S..Microbial conversion of D-xylose to xylitol[J].Ferment.Bioeng.,1998,86:1-14
[63]Mussatto S.I..Influencia do Tratamento do Hidrolisado Hemicelul(?)osico de Palha de Arroz na Producao de Xilitol por Candlda guilliermondii.M.Sc.thesis,Faculdade de Engenharia Qu(?)mica de Lorena,Brasil.2002
[64]Larsson S..Comparison of different methods for the detoxification of lignocellulose hydrolysates of spruce.Appl.Biochem.Biotechnol.,1999:77-79,91-103
[65]Jonsson L.J.,Palmqvist E.,Nilvebrant,N.O.,Hahn-nagerdal B..Detoxification of wood hydrolysates with laccase and peroxidase from the white-rot fungus Tramete5versicolor.Appl.Microbiol.Biotechnol.,1998,49:691-697
[66]Schneider H..Selective removal of acetic acid from hardwood spent sulfite liquor using a mutant yeast.Enzyme Microb.Technol.,1996,19:94-98
[67]Felipe M.G.A.,Alves,L.A..Fermentation eucalyptus hemicellulosic hydrolyzate to xylitol by Candida gvilliermondii.Bioresource Technol.,1996,56:281-283
[68]Silva C.J.S.M.,Roberto I.C..Improvement of xylitol production by Candida guilliermondii FTI 20037 previously adapted to rice straw hemicellulosic hydrolysate.Lett.Appl.Microbiol.,2001,32:248-252
[69]Sene L..Metabolic study of the adaptation of the yeast Candida guilliermondii to sugar cane bagasse hydrolysate.Appl.Microbicl.Biotechnol.,2001,57:738-743
[70]Parajo J.C.Improved xylitol production with Debaryomyces hansenii Y-7426 from raw or detoxified wood hydrolysates.Enzyme Microb.Technol.,1997,21:18-24
[71]Converti A.,Dominguez J.M..Wood hydrolysis and hydrolyzate detoxification for subsequent xylitol production.Chem.Eng.Technol.,2000,23:1013-1020
[72]Rodrigues R.C.L.B.,Felipe M.G.A..The influence of pH,temperature and hydrolysate concentration on the removal of volatile and nonvolatile compounds from sugarcane bagasse hemicellulosic hydrolysate treated with activated charcoal before or after vacuum evaporation.Braz.J.Chem.Eng.,2001,18:299-311
[73]Van Zyl C..Production of ethanol from sugar cane bagasse hemicellulose hydrolysate by Pichia stipitis.Appl.Biochem.Biotechnol.,1998,17:357-369
[74]Martinez A.,Rodriguez M.E..Detoxification of dilute acid hydrolysates of lignocellulose with lime.Biotechnol.Progr.,2001,17:287-293
[75]Silva S.S..Xylitol production by Candida guilliermondii FTI 20037 grown in pretreated sugarcane hydrolysate.Sustain.Agr.Food Energ.Ind.,1998,1116-1119
[76]Mussatto S.I..Hydrolysate detoxification with activated charcoal for xylitol production by Candida guilliermondii.Biotechnol.Lett.,2001,23:1681-1684
[77]余志晟,张洪勋.纤维素热解液发酵制乙醇.过程工程学报,2002,2(6):553-557
[78]Lee W.G..Ethanol production using concentrated oak wood hydrolysates and methods to detoxify.Appl.Biochem.Biotechnol.,1999:77-79,547-559
[79]张扬,王运红,邓立红等.稻秸半纤维素水解条件和水解液脱毒的研究.纤维素科学与技术,2005,13(2):38-44
[80]Gong C.S.Pretreatment of sugar cane bagasse hemicellulose hydrolyzate for ethanol production by yeast.Appl.Biochem.Biotechnol.,1993:39-40,83-88
[71]李增新,张启君.天然沸石及其在农业中的应用.农业环境保护,1995,14(1):41-42
[82]Godia F,Casas C,Sola C.A survey os continuous ethanol fermentation in immobilized cell Process Biochemistry,1987,22(2):43-47
[83]刘伯元.冶金部华东地勘局矿产品开发研究所沸石及其开发应用.地质与勘探,1994,9(5):21-25
[84]Fox C.R.,Kennedy D.C..Conceptual design of adsorption systems.In:Slejko F.L.(Ed.),Adsorption Technology:A Step-by-Step Approach to Process Evaluation and Application.New York,1985:91-166
[85]Bernardin Jr..Experimental design and testing of adsorption and adsorbates.In:Slejko,F.L.(Ed.),Adsorption Technology:A Step-by-Step Approach to Process Evaluation and Application.New York,1985:37-90
[86]Parajo J.C.,Dominguez H..Study of charcoal adsorption for improving the production of xylitol from wood hydrolysates.Bioprocess Eng.,1995,16:39-43
[87]Gurgel P.V.,Mancillha I.M.,Pecanha R.P..Xylitol recovery from fermented sugarcane bagasse hydrolyzate.Bioresource Technol.,1995,52:219-223
[2]张雷.中国能源供应的调整.中国能源,1999,3:12-14
[3]闫长乐.中国能源发展报告[M].北京:经济管理出版社,1994
[4]刘卿,胡迎春.21世纪初我国石油安全战略选择.国土资源科技管理,2002,6:25-30
[5]郭云涛.国家能源安全及能源管理体制面临的挑战及对策.中国煤炭,2002,28(9):4-8
[6]郭祥冰.世界可再生能源发展状况和我国发展目标.福建能源开发与节约,2001,3:6-9
[7]L.Nicole,X.Youkun.Production of ethanol from recycled paper sludge using cellulose and yest Kluverichia Coli.Biotechnology Progress,1997,12(2):135-143
[8]傅其军.燃料乙醇的发展前景.广西轻工业,2001,2:6-10
[9]M.Jose,R.Goldemberg Jose.Alcohol program Energy Policy,1999,27(4):229-245
[10]余燕春.利用农业废弃物生产酒精的社会效益[J].江西林业科,1996,6:18-20
[11]黄治玲.燃料乙醇工业的全球发展概况[J].化工管理,2002,3:34-35
[12]刘全根.乙醇汽油的应用[J]炼油设计,2001,32(2):40-45
[13]曲音波,高培基.造纸厂废物发酵生产纤维素酶、酒精和酵母综合工艺的研究进展[J].食品与发酵工业,1993,19(6):62-65.
[14]Nick Nagie.A Process Economic to Develop a Dilute-Acid Cellulose Hydrolysis process to produce ethanol from Biomass[J].Appl.Biotechnol.,1999,77-79:599-607.
[15]Jeewon Lee..Biological conversion of lignocellosic biomass to ethanol [J].J.Biotechnol.,1997,56:1-24
[16]孙玉英,谭海刚.发酵木糖酵母菌株的选育.广州食品工业科技,2003,18(4):1-4
[17]Gregg D.,Saddler J.N..Bioconversion of lignocellulosi-biomass to ethanol.Biomass and Bioenergy,1995,9(1-5):287-302
[18]H.Krrishna,K.Prasanthi.Simultaneous saccharification and fermentation of pretreated sugar cane leaves to ethanol[J].Process Biochemistry,2001,(8):825-830
[19]曲音波.开展生物制转化酒精研究实现液体燃料可持续供应 济南:中国资源生物技术与糖工程学术研讨会论文集
[20]吴坤等.木质素生物降解进展.河南农业大学学报,2000,34(4):349-354
[21]鲍晓明.嗜热细菌木糖异构酶基因XYLA在酿酒酵母中的高效表达.微生物学报,1999,39(1):49-54
[22]宋安东.生物质(秸秆)纤维燃料乙醇生产工艺试验研究[D].郑州:河南农业大学,2003
[23]沈翌等.酿酒酵母工业菌株中Ⅺ木糖代谢途径的建立.中国生物工程杂志,2005,25(9):69-73
[24]鲍晓明.酒精生产酵母菌株木糖代谢工程及其发酵工艺.济南:中国资源生物技术与糖工程学术研讨会论文集.2005
[25]勇强等.玉米秸秆生物转化制取酒精的中间试验.济南:中国资源生物技术与糖工程学术研讨会论文集.2005
[26]邓良伟.纤维素类物质生产燃料酒精研究进展.食品与发酵工业,1995,6:69-72
[27]黄忠乾,龙章富等.农作物秸秆资源的综合利用[J].资源开发与市场,2002(2):32-34
[28]陈艳萍等.戊糖发酵微生物及选育[J].纤维素科学与技术,2001,9(3):57-61
[29]Wang P Y,Shopsis C,Schneider H.Biochem Biophys Res Commun,1980,94:248-254
[30]马文漪,杨柳燕,程树培.废物乙醇化处理.环境科学进展,1994,(2)3:58-63
[31]孙可伟.固体废弃物资源化的现状和展望[J].中国资源综合利用,20(1):10-13
[32]张继泉等.预处理玉米秸杆生产燃料酒精的研究.广州食品工业科技,2002,18(3):1-3
[33]张书祥,肖亚中等.添加营养盐对酒精酵母发酵的影响.生物学杂志1997,114(1):23-25
[34]Honwy,Stcvis P,roscnfcld S et al,Biotechnol bioeng symp,1983,13:245-255.
[35]Lawford,Rousseau.Improving fermentation performance of Recombinant Zymomonas in acetic acid-containing media.Appl.Biochem.Biotechnol.1998,70-72,161-172
[36]Gong C.S.,GT.Conversion of D-xylose to ethanol by yeast.Eidited by Duck worgh H.E.and Thompson,E.A.,1983,525-555
[37]Shipra D.,Mita B..Production of alcohol fron starch by immobilized cell of saccharomyces diasrtaricus in batch and xomtinuous process.Process Biochemistry,1991,25(2):43-45
[38]Jeffrices T.W.Emerging technology for fermenting D-xylose.Trends in Biotechnology,1985,3(8):208-211
[39]Mohammad J.Taherzadeh,Claes Niklasson et al.Conversion of dilute-acid hydrolyzates of spruce and birch to ethanol by fed-batch fermentation.Bioresource technology[J],1999,69:59-66
[40]You K.M.,Rosenfield C.L.,Knipple D.C..Ethanol tolerance in the yeast Saccharomyces cerevisiae is dependent on cellular oleic acid content.Appl Environ Microbiol,2003,69(3):1499-1503
[41]沈楠,肖冬光.大豆脂肪酸对酿酒酵母酒精耐性的影响.酿酒科技,2006,11:17-20
[42]A.R.Barber,H.Hansson.Acetaldehyde stimulation of the growth of Saccharomyces cerevisiae in the presence of inhibitors found in lignocellulose-to-ethanol fermentations,Journal of Industrial Microbiology,2000,20,104-108
[43]G.A.Stanley,N.G.Douglas,E.J.Every,T.Tzanatosand N.B.Pamment.BIOTECHNOLOGY LETTERSVolume,1993,15(12):1199-1204
[44]Taherzadeh M.J.,Niklasson C.,Liden G..On-line control of fed-batch fermentation of dilute-acid hydrolyzates.Biotechnol Bioeng,2000,69:330-338
[45]Olsson L.,Hahn-HEagerdal B..Fermentation of lignocellulosic hydrolysates for ethanol production.Enzyme Microb.Technol.,1996,18:312-331
[46]Parajro J.C.,Domringuez H..Charcoal adsorption of wood hydrolysates for improving their fementability:Influence of the operational conditions.Bioresource Technol.,1996,57:179-185
[47]Palmqvist E.,Hahn-HEagerdal B..Fermentation of lignocellulosic hydrolysates.Ⅱ:Inhibitors and mechanisms of inhibition.Bioresource Technol.,2000,74:25-33
[48]Roberto I.C..Utilization of sugarcane bagasse hemicellulosic hydrolysate by Pichia stipitis for the production of ethanol.Process Biochem.,1991,26:15-21
[49]Delgenes J..Effects of lignocellulose degradation products on ethanol fermentations of glucose and xylose by Saccharomyces cerevisiae,Zymomonas mobilis,Pichia stipitis and Candida shehatae.Enzyme Microb.Technol.,1996,19:220-225
[50]Nigam J.N..Ethanol production from wheat straw hemicellulose hydrolysate by Pichia stipitis.J.Biotechnol.,2001,87:17-27
[51]Alves L.A..Pretreatment of cane bagasse hemicellulose hydrolyzate for xylitol production by Candida guilliermondii.Appl.Biochem.Biotechnol.,1998,70-72,89-98
[52]Martinez A..Effects of Ca(OH)_2 treatments on the composition and toxicity of bagasse hemicellulose hydrolysates.Biotechnol.Bioeng.,2000,69:526-536
[53]Parajro J.C..Biotechnological production of xylitol Part 3:Operation in culture media made from lignocellulose hydrolysates.Bioresource Technol.,1998,66:25-40
[54]Villa P..Influence of phenolic compounds on the bioprocess of xylitol production by Candida guilliermondii.In:Esbes-2 European Symposium On Biochemical Engineering Science,2.Porto-Portugal,1998
[55]McMillan J.D..Pretreatment of lignocellulosic biomass.In:Himmel M.E.,Baker J.O.,Overend R.P.(Eds.).Conversion of Hemicellulose Hydrolyzates to Ethanol.American Chemical Society Symposium,Washington DC,1994:292-324
[56]Van Zyl C.,Prior B.A.,du Preez J.C..Acetic acid inhibition of D-xylose fermentation by Pichia stipitis.Enzyme Microb.Technol.,1991,13:82-86
[57]Felipe M.G.A..Veira M.V..Effect of acetic acid on xylose fermentation to xylitol by Candida guilliermondii[J].Basic Microb.,1995,35:171-177
[58]Palmqvist E..Main and interaction effects of acetic acid,furfural and phydroxybenzoic acid on growth and ethanol productivity of yeasts.Biotechnol. Bioeng.,1999,63:46-55
[59]Watson N.E..Factors an acid treated bagasse inhibiting ethanol production from Dxylose by Pachysolen tannophilus.Enzyme Microb.Technol.,1984,6:451-456
[60]Roberto I.C..Utilization of cane bagasse hemicellulosic hydrolysate by Candida guilliermondii for xylitol production.Bioresource Technol.,1991,36:271-275
[61]Kuhad R.C.,Singh A..Lignocellulose biotechnology:Current and future prospects.Crit.Rev.Biotechnol.,1993,13:151-172
[62]Winkelhausen E.,guzmanova S..Microbial conversion of D-xylose to xylitol[J].Ferment.Bioeng.,1998,86:1-14
[63]Mussatto S.I..Influencia do Tratamento do Hidrolisado Hemicelul(?)osico de Palha de Arroz na Producao de Xilitol por Candlda guilliermondii.M.Sc.thesis,Faculdade de Engenharia Qu(?)mica de Lorena,Brasil.2002
[64]Larsson S..Comparison of different methods for the detoxification of lignocellulose hydrolysates of spruce.Appl.Biochem.Biotechnol.,1999:77-79,91-103
[65]Jonsson L.J.,Palmqvist E.,Nilvebrant,N.O.,Hahn-nagerdal B..Detoxification of wood hydrolysates with laccase and peroxidase from the white-rot fungus Tramete5versicolor.Appl.Microbiol.Biotechnol.,1998,49:691-697
[66]Schneider H..Selective removal of acetic acid from hardwood spent sulfite liquor using a mutant yeast.Enzyme Microb.Technol.,1996,19:94-98
[67]Felipe M.G.A.,Alves,L.A..Fermentation eucalyptus hemicellulosic hydrolyzate to xylitol by Candida gvilliermondii.Bioresource Technol.,1996,56:281-283
[68]Silva C.J.S.M.,Roberto I.C..Improvement of xylitol production by Candida guilliermondii FTI 20037 previously adapted to rice straw hemicellulosic hydrolysate.Lett.Appl.Microbiol.,2001,32:248-252
[69]Sene L..Metabolic study of the adaptation of the yeast Candida guilliermondii to sugar cane bagasse hydrolysate.Appl.Microbicl.Biotechnol.,2001,57:738-743
[70]Parajo J.C.Improved xylitol production with Debaryomyces hansenii Y-7426 from raw or detoxified wood hydrolysates.Enzyme Microb.Technol.,1997,21:18-24
[71]Converti A.,Dominguez J.M..Wood hydrolysis and hydrolyzate detoxification for subsequent xylitol production.Chem.Eng.Technol.,2000,23:1013-1020
[72]Rodrigues R.C.L.B.,Felipe M.G.A..The influence of pH,temperature and hydrolysate concentration on the removal of volatile and nonvolatile compounds from sugarcane bagasse hemicellulosic hydrolysate treated with activated charcoal before or after vacuum evaporation.Braz.J.Chem.Eng.,2001,18:299-311
[73]Van Zyl C..Production of ethanol from sugar cane bagasse hemicellulose hydrolysate by Pichia stipitis.Appl.Biochem.Biotechnol.,1998,17:357-369
[74]Martinez A.,Rodriguez M.E..Detoxification of dilute acid hydrolysates of lignocellulose with lime.Biotechnol.Progr.,2001,17:287-293
[75]Silva S.S..Xylitol production by Candida guilliermondii FTI 20037 grown in pretreated sugarcane hydrolysate.Sustain.Agr.Food Energ.Ind.,1998,1116-1119
[76]Mussatto S.I..Hydrolysate detoxification with activated charcoal for xylitol production by Candida guilliermondii.Biotechnol.Lett.,2001,23:1681-1684
[77]余志晟,张洪勋.纤维素热解液发酵制乙醇.过程工程学报,2002,2(6):553-557
[78]Lee W.G..Ethanol production using concentrated oak wood hydrolysates and methods to detoxify.Appl.Biochem.Biotechnol.,1999:77-79,547-559
[79]张扬,王运红,邓立红等.稻秸半纤维素水解条件和水解液脱毒的研究.纤维素科学与技术,2005,13(2):38-44
[80]Gong C.S.Pretreatment of sugar cane bagasse hemicellulose hydrolyzate for ethanol production by yeast.Appl.Biochem.Biotechnol.,1993:39-40,83-88
[71]李增新,张启君.天然沸石及其在农业中的应用.农业环境保护,1995,14(1):41-42
[82]Godia F,Casas C,Sola C.A survey os continuous ethanol fermentation in immobilized cell Process Biochemistry,1987,22(2):43-47
[83]刘伯元.冶金部华东地勘局矿产品开发研究所沸石及其开发应用.地质与勘探,1994,9(5):21-25
[84]Fox C.R.,Kennedy D.C..Conceptual design of adsorption systems.In:Slejko F.L.(Ed.),Adsorption Technology:A Step-by-Step Approach to Process Evaluation and Application.New York,1985:91-166
[85]Bernardin Jr..Experimental design and testing of adsorption and adsorbates.In:Slejko,F.L.(Ed.),Adsorption Technology:A Step-by-Step Approach to Process Evaluation and Application.New York,1985:37-90
[86]Parajo J.C.,Dominguez H..Study of charcoal adsorption for improving the production of xylitol from wood hydrolysates.Bioprocess Eng.,1995,16:39-43
[87]Gurgel P.V.,Mancillha I.M.,Pecanha R.P..Xylitol recovery from fermented sugarcane bagasse hydrolyzate.Bioresource Technol.,1995,52:219-223