团头鲂三种细胞系的建立、鉴定及其初步应用
|
摘要
团头鲂(Megalobrama amblycephala),俗称武昌鱼,隶属于鲤形目,鲤科,鳊亚科,鲂属,原产于我国,起初分布于长江中下游附属的湖泊,自1964年驯化成功后,因其存活率高、生长快、食草性、营养价值高、病害少和易捕捞等优点,现已推广到全国各地养殖。近年来,由于过度捕捞、水域污染和近亲交配,团头鲂种质出现严重退化和混杂现象,养殖群体出现性早熟、生长速度减慢和疾病增多等。目前对团头鲂的研究集中在生物学、免疫、疾病、营养及遗传育种等多个方面,而对团头鲂细胞培养方面的研究报道还很匮乏。因此本研究建立了团头鲂鳍条、肌肉和心脏细胞系,对3种细胞进行鉴定,并将其初步应用于病毒学、环境毒物检测和遗传学等领域。以期将这3种细胞系作为体外体系运用于团头鲂各方面的研究,为团头鲂的养殖和育种提供基础资料；运用于环境毒物检测,以确立敏感合适的细胞体外模型应用于毒物毒性的早期预警研究。具体内容及研究结果如下：
(1)团头鲂三种细胞系的建立与鉴定
用组织块贴壁法建立了团头鲂鳍条、肌肉和心脏细胞系,在一年多的时间内分别传代到95代、86代和71代以上,分别命名为MAF、MAM和MAH。3-6个月冻存后,MAF、MAM和MAH细胞复苏存活率分别为92.92±5.38%,90.04±5.49%和91.54±6.55%。MAF、MAM和MAH细胞适宜的生长温度为28-32-C,生长培养基为M199,培养基中血清浓度的添加量为10%。第10代的MAF、MAM和MAH细胞染色体数目为48分别占62%、59%和65%,说明3种细胞属正常二倍体细胞。30代MAF、MAM和MAH细胞的12s rRNA片段序列与已报道的团头鲂12srRNA序列一致率为99%-100%,表明3种细胞确实来自于团头鲂。60代MAF、MAM和MAH细胞与细胞角蛋白,纤连蛋白,结蛋白和Ki67抗体均呈阳性反应,表明3种细胞增殖能力强,呈现出上皮样和成纤维样混合状。
(2)团头鲂鳍条细胞的初步应用
以MAF细胞为研究对象,测定黑鱼棒状病毒(SHRV),鲤春病毒血症病毒(SVCV),斑点叉尾鮰病毒(CCV)和草鱼呼肠孤病毒(GCRV)对MAF细胞的敏感性实验。结果表明,MAF细胞对SHRV, SVCV和CCV较敏感,分别在感染后的1d、2d和2d出现典型的病变；MAF细胞对GCRV不敏感。PCR技术确认了MAF细胞被SHRV、SVCV和CCV感染,而不能被GCRV感染。SHRV、 SVCV和CCV在MAF细胞中病毒滴度分别达到108.2,106.8和105.9TCID50ml-1。MAF细胞对嗜水气单胞菌胞外产物敏感,24h细胞的形态即发生改变,细胞皱缩、变圆,最终整个单层脱落。MAF细胞对重金属铜也很敏感,24h半致死浓度为144.48±13.25μmol/L。用脂质体2000能成功将pEGFP-Nl转入到MAF细胞中,转染效率为5%。上述结果表明MAF作为体外体系能应用于病毒学、细胞生物学和遗传学等领域。
(3)全氟辛酸对团头鲂肌肉细胞的毒性研究
采用MTT法、细胞总蛋白含量(CP)、中性红摄取试验(NR)法和CCK-8试剂盒测定全氟辛酸对MAM细胞的毒性。实验结果表明：设定的全氟辛酸浓度都对MAM细胞的生长有抑制作用,且细胞存活率的降低与全氟辛酸浓度呈正相关。24h IC50值分别为240.81±10.66mg/L、243.96±11.65mg/L、248.63±13.95mg/L和223.62±13.97mg/L,48h IC50值分别为214.01±10.48mg/L、219.86±10.13mg/L、235.57±8.43mg/L和210.40±8.32mg/L,4种方法测定24h和48h IC50值的大小顺序为NR> CP>MT1>CCK-8,经分析4种方法测定的24h和48h ICso值没有显著性差异(P>0.05)。50mg/L和100mg/L全氟辛酸对MAM细胞暴露染毒后48h,MAM细胞不饱满,有皱缩的趋势,100mg/L组更明显；扫描电镜结果显示：50mg/L全氟辛酸暴露后,MAM细胞核变小,细胞中脂滴数量有增多的趋势,线粒体膨大,100mg/L组MAM细胞中的脂滴数量明显增加,线粒体膨大可以看到明显的嵴。表明全氟辛酸对MAM细胞的线粒体有损伤,并对其脂质代谢有影响。
Blunt snout bream (Megalobrama amblycephala), commonly known as Wuchang fish, affiliated to Cypriniformes, Cyprinidae, Abramidinae, Megalobrama. M. amblycephala is native to China, distributed originally to affiliated lakes of middle and lower reaches of the Yangtze River in China. In1964it was domesticated successfully, endowed with excellent biological characteristics for high survival rate, fast growth, herbivorous, high nutritive value, less disease and easy fishing, etc., now it has become one of the main farmed freshwater species in China. In recent years, the germplasm resources of M. amblycephala are under threat of recession and mixture due to overfishing, pollution of waters, inbreeding, and cultured population of M. amblycephala is gradually exhibiting early such as smaller sexual maturity individuals, lose of growth predominance and increase disease. Now, many studies have been conducted to understand its biology, immunology, nutrition, diseases and breeding, but there is limited information available on cultured cells derived from M. amblycephala. Therefore, this study established and characterized three new fish cell lines from the fin, muscle and heart of M. amblycephala, and used these three cell lines as in vitro systems for studying virology, environmental toxicology, genetics and so on. We hope these three cell lines as in vitro systems could be applied in all researches of M. amblycephala, provid basic datas for farming and breeding of M. amblycephala, and identify the more sensitive cell lines as bio-indicators to detect environmental toxic chemicals. The main results were as follows:
(1) Development and characterization of three new cell lines from M. amblycephala
Three new fish cell lines (MAF、MAM and MAH) were developed from the fin, muscle and heart of M. amblycephala by using the tissue explants technique, and have been subcultured more than95,86and71times in more than a year. After3-6months of storage in liquid nitrogen, the viability of the MAF, MAM and MAH cells was92.92±5.38%,90.04±5.49%and91.54±6.55%, respectively. All the cell lines were optimally maintained at28-32℃in medium199supplemented with10%fetal bovine serum. Most MAF, MAM and MAH cells have chromosome number of48at passage10, which accounted for62%,59%and65%, respectively, it demonstrated that the three cells are normal diploid cells. DNA was isolated from passage30MAF, MAM and MAH cells, an expected PCR product size was obtained by amplification of12s rRNA from extracted DNA, subsequent comparative analysis of the identified sequences demonstrated a99-100%match with the known mitochondrial DNA sequence of M. amblycephala12S rRNA, the results demonstrated that MAF, MAM and MAH cells indeed originated from M. amblycephala. MAF, MAM and MAH cells were strongly positive for pancytokeratin, desmin, fibronectin and the proliferative marker Ki67, the results demonstrated the three cells to be fibroblastic, epithelial and muscular in nature.
(2) The preliminary application of MAF cells
The susceptibility of MAF cells to infection by four viruses, snakehead rhabdovirus (SHRV), spring viremia carp virus (SVCV), channel catfish virus (CCV) and grass carp reovirus (GCRV), was evaluated by cytopathic effects (CPE). The significant CPE of SHRV, SVCV and CCV infection was observed in MAF cells at1d,2d and2d post-inoculation, respectively. However, no CPE was found in GCRV-infected MAF cells. Furthermore, PCR assays detected prominent bands from SVCV, SHRV and CCV infected MAF cells, it demonstrated MAF cells infected by SVCV, SHRV and CCV. The viral titers of SVCV, SHRV and CCV in MAF cells reached1068,1082and1O5.9TCID50ml-1, respectively. Bacterial cytotoxicity studies showed that extracellular products from Aeromonas hydrophila were toxic to MAF cells, the morphological changes were shrinking, detachment and finally destruction of the monolayer. Cu2+was also cytotoxic to MAF cells and the24h-IC50value was144.48±3.25μmol/L. When MAF cells were transfected with pEGFP-N1plasmid, bright fluorescent signals were observed, and the transfection efficiency reached up to5%. These results suggest that the MAF cell line may provide a valuable tool for studying virus pathogenesis, as well as cytotoxicity testing and genetic manipulation studies.
(3) The toxicity studies of perfluorooctanoic acid on MAM cells
Cytotoxicity of perfluorooctanoic acid (PFOA) on MAM cells was measured by four endpoint systems:methyl thiazolyl tetrazolium (MTT) assay, cell protein (CP) assay, neutral red (NR) uptake assay and cell counting kit-8(CCK-8) assay. The result showed that PFOA was cytotoxic to MAM cells at all tested concentrations, and toxicity increased as the concentration of PFOA was progressively increased. The24h-IC50values of PFOA were240.81±10.66mg/L,243.96±11.65mg/L,248.63±13.95mg/L and223.62±13.97mg/L for MTT assay, CP assay, NR uptake and CCK-8assay, respectively. The48h-IC50values were214.01±10.48mg/L,219.86±10.13mg/L,235.57±8.43mg/L and210.40±8.32mg/L, respectively. For the four methods,24h-IC50and48h-IC50values were NR>CP>MTT>CCK-8, and there was no significant difference for24h-IC50and48h-IC50values measured by these foure methods (P>0.05). Moreover, the morphological changes of MAM cells were also studied at the concentration of50mg/L and100mg/L for48h. MAM cells were not full, tended to shrinking, and100mg/L group was more obvious. Under a scanning electron microscopy, the nucleus of MAM cells became small, mitochondrial swelled and an increased number of lipid particles after50mg/L PFOA exposure. The number of lipid droplets in MAM cells was significantly increased, and mitochondrial swelled with obvious ridge could be seen in MAM cells at the group of100mg/L. These results indicated PFOA could damage mitochondrial and affect lipid metabolism of MAM cells. This would suggest that the MAM cell line is a suitable bioindicator for the screening of the acute toxicity of PFOA.
(1)团头鲂三种细胞系的建立与鉴定
用组织块贴壁法建立了团头鲂鳍条、肌肉和心脏细胞系,在一年多的时间内分别传代到95代、86代和71代以上,分别命名为MAF、MAM和MAH。3-6个月冻存后,MAF、MAM和MAH细胞复苏存活率分别为92.92±5.38%,90.04±5.49%和91.54±6.55%。MAF、MAM和MAH细胞适宜的生长温度为28-32-C,生长培养基为M199,培养基中血清浓度的添加量为10%。第10代的MAF、MAM和MAH细胞染色体数目为48分别占62%、59%和65%,说明3种细胞属正常二倍体细胞。30代MAF、MAM和MAH细胞的12s rRNA片段序列与已报道的团头鲂12srRNA序列一致率为99%-100%,表明3种细胞确实来自于团头鲂。60代MAF、MAM和MAH细胞与细胞角蛋白,纤连蛋白,结蛋白和Ki67抗体均呈阳性反应,表明3种细胞增殖能力强,呈现出上皮样和成纤维样混合状。
(2)团头鲂鳍条细胞的初步应用
以MAF细胞为研究对象,测定黑鱼棒状病毒(SHRV),鲤春病毒血症病毒(SVCV),斑点叉尾鮰病毒(CCV)和草鱼呼肠孤病毒(GCRV)对MAF细胞的敏感性实验。结果表明,MAF细胞对SHRV, SVCV和CCV较敏感,分别在感染后的1d、2d和2d出现典型的病变；MAF细胞对GCRV不敏感。PCR技术确认了MAF细胞被SHRV、SVCV和CCV感染,而不能被GCRV感染。SHRV、 SVCV和CCV在MAF细胞中病毒滴度分别达到108.2,106.8和105.9TCID50ml-1。MAF细胞对嗜水气单胞菌胞外产物敏感,24h细胞的形态即发生改变,细胞皱缩、变圆,最终整个单层脱落。MAF细胞对重金属铜也很敏感,24h半致死浓度为144.48±13.25μmol/L。用脂质体2000能成功将pEGFP-Nl转入到MAF细胞中,转染效率为5%。上述结果表明MAF作为体外体系能应用于病毒学、细胞生物学和遗传学等领域。
(3)全氟辛酸对团头鲂肌肉细胞的毒性研究
采用MTT法、细胞总蛋白含量(CP)、中性红摄取试验(NR)法和CCK-8试剂盒测定全氟辛酸对MAM细胞的毒性。实验结果表明：设定的全氟辛酸浓度都对MAM细胞的生长有抑制作用,且细胞存活率的降低与全氟辛酸浓度呈正相关。24h IC50值分别为240.81±10.66mg/L、243.96±11.65mg/L、248.63±13.95mg/L和223.62±13.97mg/L,48h IC50值分别为214.01±10.48mg/L、219.86±10.13mg/L、235.57±8.43mg/L和210.40±8.32mg/L,4种方法测定24h和48h IC50值的大小顺序为NR> CP>MT1>CCK-8,经分析4种方法测定的24h和48h ICso值没有显著性差异(P>0.05)。50mg/L和100mg/L全氟辛酸对MAM细胞暴露染毒后48h,MAM细胞不饱满,有皱缩的趋势,100mg/L组更明显；扫描电镜结果显示：50mg/L全氟辛酸暴露后,MAM细胞核变小,细胞中脂滴数量有增多的趋势,线粒体膨大,100mg/L组MAM细胞中的脂滴数量明显增加,线粒体膨大可以看到明显的嵴。表明全氟辛酸对MAM细胞的线粒体有损伤,并对其脂质代谢有影响。
Blunt snout bream (Megalobrama amblycephala), commonly known as Wuchang fish, affiliated to Cypriniformes, Cyprinidae, Abramidinae, Megalobrama. M. amblycephala is native to China, distributed originally to affiliated lakes of middle and lower reaches of the Yangtze River in China. In1964it was domesticated successfully, endowed with excellent biological characteristics for high survival rate, fast growth, herbivorous, high nutritive value, less disease and easy fishing, etc., now it has become one of the main farmed freshwater species in China. In recent years, the germplasm resources of M. amblycephala are under threat of recession and mixture due to overfishing, pollution of waters, inbreeding, and cultured population of M. amblycephala is gradually exhibiting early such as smaller sexual maturity individuals, lose of growth predominance and increase disease. Now, many studies have been conducted to understand its biology, immunology, nutrition, diseases and breeding, but there is limited information available on cultured cells derived from M. amblycephala. Therefore, this study established and characterized three new fish cell lines from the fin, muscle and heart of M. amblycephala, and used these three cell lines as in vitro systems for studying virology, environmental toxicology, genetics and so on. We hope these three cell lines as in vitro systems could be applied in all researches of M. amblycephala, provid basic datas for farming and breeding of M. amblycephala, and identify the more sensitive cell lines as bio-indicators to detect environmental toxic chemicals. The main results were as follows:
(1) Development and characterization of three new cell lines from M. amblycephala
Three new fish cell lines (MAF、MAM and MAH) were developed from the fin, muscle and heart of M. amblycephala by using the tissue explants technique, and have been subcultured more than95,86and71times in more than a year. After3-6months of storage in liquid nitrogen, the viability of the MAF, MAM and MAH cells was92.92±5.38%,90.04±5.49%and91.54±6.55%, respectively. All the cell lines were optimally maintained at28-32℃in medium199supplemented with10%fetal bovine serum. Most MAF, MAM and MAH cells have chromosome number of48at passage10, which accounted for62%,59%and65%, respectively, it demonstrated that the three cells are normal diploid cells. DNA was isolated from passage30MAF, MAM and MAH cells, an expected PCR product size was obtained by amplification of12s rRNA from extracted DNA, subsequent comparative analysis of the identified sequences demonstrated a99-100%match with the known mitochondrial DNA sequence of M. amblycephala12S rRNA, the results demonstrated that MAF, MAM and MAH cells indeed originated from M. amblycephala. MAF, MAM and MAH cells were strongly positive for pancytokeratin, desmin, fibronectin and the proliferative marker Ki67, the results demonstrated the three cells to be fibroblastic, epithelial and muscular in nature.
(2) The preliminary application of MAF cells
The susceptibility of MAF cells to infection by four viruses, snakehead rhabdovirus (SHRV), spring viremia carp virus (SVCV), channel catfish virus (CCV) and grass carp reovirus (GCRV), was evaluated by cytopathic effects (CPE). The significant CPE of SHRV, SVCV and CCV infection was observed in MAF cells at1d,2d and2d post-inoculation, respectively. However, no CPE was found in GCRV-infected MAF cells. Furthermore, PCR assays detected prominent bands from SVCV, SHRV and CCV infected MAF cells, it demonstrated MAF cells infected by SVCV, SHRV and CCV. The viral titers of SVCV, SHRV and CCV in MAF cells reached1068,1082and1O5.9TCID50ml-1, respectively. Bacterial cytotoxicity studies showed that extracellular products from Aeromonas hydrophila were toxic to MAF cells, the morphological changes were shrinking, detachment and finally destruction of the monolayer. Cu2+was also cytotoxic to MAF cells and the24h-IC50value was144.48±3.25μmol/L. When MAF cells were transfected with pEGFP-N1plasmid, bright fluorescent signals were observed, and the transfection efficiency reached up to5%. These results suggest that the MAF cell line may provide a valuable tool for studying virus pathogenesis, as well as cytotoxicity testing and genetic manipulation studies.
(3) The toxicity studies of perfluorooctanoic acid on MAM cells
Cytotoxicity of perfluorooctanoic acid (PFOA) on MAM cells was measured by four endpoint systems:methyl thiazolyl tetrazolium (MTT) assay, cell protein (CP) assay, neutral red (NR) uptake assay and cell counting kit-8(CCK-8) assay. The result showed that PFOA was cytotoxic to MAM cells at all tested concentrations, and toxicity increased as the concentration of PFOA was progressively increased. The24h-IC50values of PFOA were240.81±10.66mg/L,243.96±11.65mg/L,248.63±13.95mg/L and223.62±13.97mg/L for MTT assay, CP assay, NR uptake and CCK-8assay, respectively. The48h-IC50values were214.01±10.48mg/L,219.86±10.13mg/L,235.57±8.43mg/L and210.40±8.32mg/L, respectively. For the four methods,24h-IC50and48h-IC50values were NR>CP>MTT>CCK-8, and there was no significant difference for24h-IC50and48h-IC50values measured by these foure methods (P>0.05). Moreover, the morphological changes of MAM cells were also studied at the concentration of50mg/L and100mg/L for48h. MAM cells were not full, tended to shrinking, and100mg/L group was more obvious. Under a scanning electron microscopy, the nucleus of MAM cells became small, mitochondrial swelled and an increased number of lipid particles after50mg/L PFOA exposure. The number of lipid droplets in MAM cells was significantly increased, and mitochondrial swelled with obvious ridge could be seen in MAM cells at the group of100mg/L. These results indicated PFOA could damage mitochondrial and affect lipid metabolism of MAM cells. This would suggest that the MAM cell line is a suitable bioindicator for the screening of the acute toxicity of PFOA.
引文
1.陈松林,秦启伟.鱼类细胞培养理论与技术.北京：科学出版社,2011.1-69
2.崔燕,白承连,徐涛,王晓彤,陈元红,靳大庆.PFOA对斑马鱼胚胎发育、行为和DNA损伤的毒性研究(英文).生态毒理学报,2012,7(3)：241-250
3.杜玉东,陈孝煊,吴志新,王敏,刘迁,夏君,王树云,李莉娟.斑点叉尾鮰疱疹病毒PCR-ELISA检测方法的建立.华中农业大学学报,2009,28(5)：604-608
4.弗雷谢尼RI著.动物细胞培养：基本技术指南.章静波,徐存拴译.5版.北京：科学出版社,2008.30-90
5.霍静,洪锡钧,王志坚,刘小红,王咏梅.南方鲇肾细胞系(SMK-I)的建立及其细胞骨架的研究.西南大学学报(自然科学版),2007,29(12)：128-133
6.金一和,丁梅,翟成,王烈,董光辉,舒为群,张颖花,齐藤宪光,佐佐木和明.长江三峡库区江水和武汉地区地面水中PFOS和PFOA污染现状调查.生态环境,2006,15(3)：486-489
7.金一和,刘晓,李彤,秦红梅,张颖花,齐藤宪光,佐佐木和明,小泉昭夫.沈阳地区成人血清和脐带血中全氟有机物污染现状.卫生研究,2004,33(4)：481-483
8.孔祥婷,沈颂东,姜德勋,姜红霞,徐健荣,张君,韩晓磊,许璞.黄颡鱼吻端细胞培养研究.淡水渔业,2009,39(2)：19-25
9.李思发,蔡完其.团头鲂双向选育效应研究.水产学报,2000,24(3)：201-205
10.李思发,杨学明.双向选择对团头鲂生化遗传变异的影响.中国水产科学,1996,3(1)：1-5
11.李文峰,麦康森,黄倢,史成银.鱼类细胞培养及其在病毒学研究中的应用.动物医学进展,2010,35(1)：107-110
12.李杨.团头鲂三个野生群体的遗传结构分析及遗传图谱的构建.[博士学位论文].武汉：华中农业大学图书馆,2010
13.刘肖莲.鳜鱼与团头鲂微卫星标记的应用及分子育种基础研究.[博士学位论文].武汉：华中农业大学图书馆,2012
14.刘新建,李贵生.国内鱼类病毒病研究进展.生态科学,2004,23(3)：282-285
15.卢向明,陈萍萍.低剂量全氟辛酸对雄性黑斑蛙生殖毒效应及机理研究.环境科学学报,2012,32(6)：1497-1502
16.冉玮,张桂蓉,王卫民,魏开建,周玲玲.利用SRAP标记分析3个团头鲂群体的遗传多样性.华中农业大学学报,2010,29(5)：601-606.
17.任秉新.大菱鲆(Scophthalmus maximus)肝细胞体外培养、红体病虹彩病毒体外繁殖条件及病毒疫苗研制的研究.[博士学位论文].青岛：中国海洋大学图书馆,2009
18.任国诚,陈松林,沙珍霞.半滑舌鳎肝脏细胞系的建立与鉴定.高技术通讯,2008,18(6)：657-660
19.沈锦玉.嗜水气单胞菌外膜提取物及胞外产物特性的分析.[硕士学位论文].杭州：浙江大学图书馆,2002
20.谭凤霞,王敏,王卫民.利用草鱼CIK细胞和MTT法测定镉和铬毒性试验的优化.水生生物学报,2006,30(3)：371-374
21.谭凤霞,杨方星,王卫民,王敏.稀有鮈鲫鳍细胞系的建立及其作为测定重金属毒性模型的探讨.水生生物学报,2009,33(4)：767-771
22.谭凤霞.三株鱼类细胞系的建立和十二株鱼类细胞系对重金属毒性的敏感性研究.[博士学位论文].武汉：华中农业大学图书馆,2008
23.唐首杰,李思发,蔡完其.团头鲂野生、驯养、选育3类遗传生态群体遗传变异的线粒体DNA分析.中国水产科学,2011,18(3)：483-492
24.王京真,姬伟,肖武汉,王丁.长江江豚脐静脉细胞体外培养的初步研究.水生生物学报,2010,34(2)：436-441
25.王铁辉,张义兵,李戈强,易泳兰,刘汉勤,朱作言.鱼类培养细胞干扰素的诱导.病毒学报,1999,15(1)：47-53
26.王贤丽.几种重要海水养殖鱼类细胞系的建立、诱导分化及其应用研究.[博士学位论文].青岛：中国海洋大学图书馆,2009
27.韦新兰,张杰,陈丽萍,王卫民,高泽霞,王焕岭.团头鲂SPATA4基因的 分子克隆及表达分析.华中农业大学学报,2012,31(6)：737-743
28.魏彦章,陆仁后,白国栋.草鱼尾鳍组织二倍体细胞系GCCF-2的建立及其部分生物学特性分析.水产学报,1987,11(4)：269-275
29.魏云波,刘书花,王兴民,周延生,孙爱.石鲽鳍细胞系的建立及三丁基锡对其毒性作用的研究.现代农业科技,2011,19：13-14,18
30.魏云波.褐点石斑鱼(Epinephelus fuscoguttatus)三种细胞系的建立、病毒敏感性以及环境污染物对其毒性作用的研究.[博士学位论文].青岛：中国海洋大学图书馆,2009
31.谢保胜,王刚,史健全,祁洪芳.青海湖裸鲤体外肝胰细胞原代与传代培养.生物学杂志,2009,26(4)：34-37
32.徐晓辉.两种星鲽组织细胞系的建立及久效磷对其毒性作用的研究.[硕士学位论文].青岛：中国海洋大学图书馆,2010
33.杨谨,李昂,杨谦,李旭.过氧化物酶体增殖剂PFOA对小鼠免疫系统的影响.细胞与分子免疫学杂志,2006,22(2)：157-160
34.杨先乐.鱼类组织培养的回顾与展望.水产学报,1999,23：74-81
35.姚晓峰,仲来福.全氟辛酸对HepG2细胞的遗传毒性及氧化性DNA损伤.毒理学杂志,2005,19(3)：216-217
36.姚晓峰.全氟辛酸对HepG2细胞的遗传毒性及氧化性DNA损伤.[硕士学位论文].大连：大连医科大学.,2006
37.叶露,吴玲玲,蒋雨希,张超杰,陈玲.PFOS/PFOA对斑马鱼(Danio rerio)胚胎致毒效应研究.环境科学,2009,30(6)：1727-1732
38.叶露.典型全氟化合物水生毒理学效应与致毒机理初探.[硕士学位论文].上海：同济大学图书馆,2008
39.尹洪滨,范兆廷,孙中武,潘峰,孟繁荣.团头鲂核型与DNA含量分析研究.水产学杂志,1995,8(1)：22-26
40.于淼,管华诗,郭华荣,张士璀.鱼类细胞培养及其应用.海洋科学,2003,27(3)：4-8
41.曾聪,阎里清,高泽霞,曹小娟,钱雪桥,王卫民.梁子湖、鄱阳湖和淤泥湖团头鲂的形态学比较.华中农业大学学报,2012,31(1)：88-94
42.曾令兵,李晓莉,张林,徐进,张燕.斑点叉尾鮰肾脏组织细胞系的建立及 其生物学特性.中国水产科学,2009,16(1)：75-81
43.张贵生,傅荣恕.稀土与铜对鲹鲦鱼红细胞微核及核异常的影响.四川动物,2007,26(3)：644-646
44.张念慈,杨广智.草鱼吻端组织细胞株ZC-7901及其亚株ZC-7901S1的建立和特性观察.水产学报,1981,5(1)：111-120
45.张奇亚.我国水生动物病毒病研究概况.水生生物学报,2002,26(1)：89-101
46.张倩,张超杰,周琪,陈玲. SPE-HPLC/MS联用法测定地表水中的PFOA及PFOS含量.四川环境,2006,25(4)：10-12,28
47.张新辉,夏新民,罗伟,高泽霞,钱雪桥,王卫民.团头鲂雌核发育后代的微卫星标记分析.华中农业大学学报,2012,31(6)：737-743
48.张新辉.团头鲂三倍体和雌核发育的诱导及其主要生物学特性的研究.[硕士学位论文].武汉：华中农业大学图书馆,2013
49.张义兵,俞小牧,陈敏容,赵庆中,陈道权,王铁辉,王丁.白鱀豚组织培养的初步研究.水生生物学报,2000,24(2)：193-195
50.中国渔业统计年鉴,北京：中国农业出版社,2001
51.左文功,钱华鑫,许映芳,杜森英,杨先乐.草鱼肾组织细胞系CIK的建立及其生物学特性.水产学报,1986,10(1)：11-17
52. Akimoto K, Takaoka T, Sorimachi K. Development of a simple culture method for the tissues contaminated with microorganisms and application to establishment of a fish cell line. Zool Sci 2000,17(1):61-63
53. Ariel E, Nicolajsen N, Christophersen M B, Holopainen R, Tapiovaara H, Bang Jensen B. Propagation and isolation of ranaviruses in cell culture. Aquaculture 2009,294(3-4):159-164
54. Aviv A. How long should telomeres be? Current hypertension reports 2001,3(2): 145-151.
55. Babu V, Nambi K, Chandra V, Ishaq Ahmed V, Bhonde R, Sahul Hameed A. Establishment and characterization of a fin cell line from Indian walking catfish, Clarias batrachus (L.). JFish Dis 2011,34(5):355-364
56. Balebona M, Krovacek K, Morinigo M, Mansson I, Faris A, Borrego J. Neurotoxic effect on two fish species and a PC12 cell line of the supernate of Vibrio alginolyticus and Vibrio anguillarum. Vet Microbiol 1998,63:61-69
57. Barker C, Rackham B. The induction of sister-chromatid exchanges in cultured fish cells (Ameca splendens) by carcinogenic mutagens. Mutat Res 1979,68(4): 381-387
58. Bayne BL, Brown DA, Burns K, Dixon DR, Ivanovici A, Livingstone DA, Lowe DM, Moore MN, Stebbing ARD, Widdings J. The effects of stress and pollution on marine animals. Praeger, New York, USA.1985
59. Bejar J, Borrego JJ, Alvarez MC. A continuous cell line from the cultured marine fish gilt-head seabream (Sparus aurata L.). Aquaculture 1997,150(1-2):143-153
60. Berthiaume J, Wallace KB. Perfluorooctanoate, perflourooctanesulfonate, and N-ethyl perfluorooctanesulfonamido ethanol; peroxisome proliferation and mitochondrial biogenesis. Toxicol Lett 2002,129(1-2):23-32
61. Beyenbach KW, Booz G, Kleinzeller A. Transepithelial voltages and resistances across isolated perfused renal tubules of the winter flounder. Bull Mt Desert Isl Biol Lab 1980,20:78-82
62. Biegel LB, Hurtt ME, Frame SR, O'Connor JC, Cook JC. Mechanisms of extrahepatic tumor induction by peroxisome proliferators in male CD rats. Toxicol Sci 2001,60(1):44-55
63. Bodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu CP, Morin GB, Harley CB, Shay JW, Lichtsteiner S, Wright WE. Extension of life-span by introduction of telomerase into normal human cells. Science 1998,279(5349):349-352
64. Bols N, Dayeh V, Lee L, Schirmer K. Use of fish cell lines in the toxicology and ecotoxicology of fish. Piscine cell lines in environmental toxicology. Biochem Mol Biol Fish 2005,6:43-84
65. Boulanger B, Vargo J, Schnoor JL, Hornbuckle KC. Detection of perfluorooctane surfactants in Great Lakes water. Environ Sci Technol 2004,38(15):4064-4070
66. Bowser PR, Plumb JA. Fish cell lines:establishment of a line from ovaries of channel catfish. In vitro 1980,16(5):365-368
67. Chen MJ, Chiou PP, Liao YH, Lin CM, Chen TT. Development and characterization of five rainbow trout pituitary single-cell clone lines capable of producing pituitary hormones. JEndocrinol 2010,205(1):69-78
68. Chen QL, Luo, Z, Liu X, Song YF, Liu CX, Zheng JL, Zhao YH. Effects of waterborne chronic copper exposure on hepatic lipid metabolism and metal-element composition in synechogobius hasta. Arch Environ Contam Toxicol 2013, 64(2):301-315
69. Chen SL, Ren GC, Sha ZX, Hong Y. Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus). Aquaculture 2005,249(1-4):63-68
70. Chen SL, Ren GC, Sha ZX, Hong Y. Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus). Aquaculture 2005,249(1-4):63-68
71. Chen SL, Ren GC, Sha ZX, Shi CY. Establishment of a continuous embryonic cell line from Japanese flounder Paralichthys olivaceus for virus isolation. Dis Aquat Org 2004,60:241-246
72. Chen SL, Sha ZX, Ye H Q. Establishment of a pluripotent embryonic cell line from sea perch(Lateolabrax japonicus) embryos. Aquaculture 2003,218(1-4): 141-151
73. Cheng LL, Bowser P, Spitsbergen J. Development of cell cultures derived from lake trout liver and kidney in a hormone-supplemented, serum-reduced medium. J Aquat Anim Health 1993,5:119-126
74. Chi S, Hu W, Lo B. Establishment and characterization of a continuous cell line (GF-1) derived from grouper, Epinephelus coioides (Hamilton):a cell line susceptible to grouper nervous necrosis virus (GNNV). J Fish Dis 1999,22(3): 173-182
75. Clem LW, Moewus L, Sigel MM. Studies with cells from marine fish in tissue culture, Proceedings of the society for experimental biology and medicine. Society for experimental biology and medicine (New York, NY). Royal Society of Medicine 1961,762-766
76. Collodi P, Kame Y, Ernst T, Miranda C, Buhler DR, Barnes DW. Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues. Cell Biol Toxicol 1992,8(1):43-61
77. Costa-Ramos C, Rowley AF. Effect of extracellular products of Pseudoalteromonas atlantica on the edible crab Cancer pagurus. Appl Environ Microbiol 2004,70(2):729-735
78. Cuchens MA, Clem LW. Phylogeny of lymphocyte heterogeneity:II. Differential effects of temperature on fish T-like and B-like cells. Cell Immunol 1977,34(2): 219-230
79. DeWitte-Orr S, Lepic K, Bryson S, Walsh S, Lee L, Bols N. Development of a continuous cell line, PBLE, from an American eel peripheral blood leukocyte preparation. In Vitro Cell Dev Biol Anim 2006,42(8-9):263-272
80. Dinglasan MJA, Ye Y, Edwards EA, Mabury SA. Fluorotelomer alcohol biodegradation yields poly-and perfluorinated acids. Environ Sci Technol 2004, 38(10):2857-2864
81. Dong C, Weng S, Shi X, Xu X, Shi N, He J. Development of a mandarin fish Siniperca chuatsi fry cell line suitable for the study of infectious spleen and kidney necrosis virus (ISKNV). Virus Res 2008,135(2):273-281
82. Ellis DA, Martin JW, De Silva AO, Mabury SA, Hurley MD, Sulbaek Andersen MP, Wallington TJ. Degradation of fluorotelomer alcohols:a likely atmospheric source of perfluorinated carboxylic acids. Environ Sci Technol 2004,38(12): 3316-3321
83. Fan L, Collodi P. Progress towards cell-mediated gene transfer in zebrafish. Briefings Funct Genomic Proteomic 2002,1(2):131-138
84. Fan TJ, Ren BX, Geng XF, Yu QT, Wang LY. Establishment of a turbot fin cell line and its susceptibility to turbot reddish body iridovirus. Cytotechnology 2010, 62(3):217-223
85. Fijan N, Sulimanovic D, Bearzotti M, Muzinic D, Zwillenberg L, Chilmonczyk S, Vautherot J, De Kinkelin P. Some properties of the Epithelioma papulosum cyprini (EPC) cell line from carp cyprinus carpio. Annales De l'Institut Pasteur-/Virologie 1983,134(2):207-220
86. Forest D, Nishikawa R, Kobayashi H, Parton A, Bayne C J, Barnes D W. RNA expression in a cartilaginous fish cell line reveals ancient 3'noncoding regions highly conserved in vertebrates. Proceedings of the National Academy of Sciences 2007,104(4):1224-1229
87. Frerichs G, Morgan D, Hart D, Skerrow C, Roberts R, Onions D. Spontaneously productive C-type retro virus infection of fish cell lines. J Gen Virol 1991,72(10): 2537-2539
88. Fryer JL, Lannan C. Three decades of fish cell culture:a current listing of cell lines derived from fishes. J Tissue Cult Meth 1994,16(2):87-94
89. Garcia DM, Bauer H, Dietz T, Schubert T, Markl J, Schaffeld M. Identification of keratins and analysis of their expression in carp and goldfish:comparison with the zebrafish and trout keratin catalog. Cell Tissue Res 2005,322(2):245-256
90. Goecke-Flora CM, Reo NV. Influence of carbon chain length on the hepatic effects of perfluorinated fatty acids. Chem Res Toxicol 1996,9(4):689-695
91. Gravell M, Malsberger R. A permanent cell line from the fathead minnow (Pimephales promelas).Ann N Y Acad Sci 1965,126(1):555-565
92. Guruge KS, Yeung LW, Yamanaka N, Miyazaki S, Lam PK, Giesy JP, Jones PD, Yamashita N. Gene expression profiles in rat liver treated with perfluoroctanoic acid (PFOA). Toxicol Sci 2006,89(1):93-107
93. Han X, Kcrmpcr RA, Jepson GW. Subcellular Distribution and protein binding of pcrfluorooctanoic acid in rat liver and kindey. Drug Chem Toxicol 2005,28(2): 197-209
94. Hashimoto H, Toyohara H, Yokoyama Y, Sakaguchi M, Ozato K, Wakamatsu Y. Effects of carp serum on the growth of goldfish fin cells in early passage. J Fish Biol 1997,50(1):201-207
95. Haughom B, Spydevold 0. The mechanism underlying the hypolipemic effect of perfluorooctanoic acid (PFOA), perfluorooctane sulphonic acid (PFOSA) and clofibric acid. Biochim BiophysActa 1992,1128(1):65-72
96. Herrmann H, Munick M, Brettel M, Fouquet B, Markl J. Vimentin in a cold-water fish, the rainbow trout:highly conserved primary structure but unique assembly properties. J cell Sci 1996,109(3):569-578
97. Hightower LE, Renfro JL. Recent applications of fish cell culture to biomedical research. JExp Zool 1988,248(3):290-302
98. Holen E, Kausland A, Skjaerven K. Embryonic stem cells isolated from Atlantic cod (Gadus morhua) and the developmental expression of a stage-specific transcription factor ac-Pou2. Fish Physio Biochem 2010,36(4):1029-1039
99. Huang X, Huang Y, Ouyang Z, Qin Q. Establishment of a cell line from the brain of grouper(Epinephelus akaara) for cytotoxicity testing and virus pathogenesis. Aquaculture 2011,311(1-4):65-73
100.Huang X, Huang Y, Sun J, Han X, Qin Q. Characterization of two grouper Epinephelus akaara cell lines:Application to studies of Singapore grouper iridovirus (SGIV) propagation and virus-host interaction. Aquaculture 2009, 292(3-4):172-179
101.Ishaq Ahmed V, Chandra V, Parameswaran V, Venkatesan C, Shukla R, Bhonde R, Sahul Hameed A. A new epithelial-like cell line from eye muscle of catla Catla catla (Hamilton):development and characterization. J Fish Biol 2008,72(8): 2026-2038
102.Ishaq Ahmed V, Chandra V, Sudhakaran R, Rajesh Kumar S, Sarathi M, Sarath Babu V, Ramesh B, Sahul Hameed A. Development and characterization of cell lines derived from rohu, Labeo rohita (Hamilton), and catla, Catla catla (Hamilton). JFish Dis 2009a,32(3):211-218
103.Ishaq Ahmed V, Sarath Babu V, Chandra V, Nambi K, Thomas J, Bhonde R, Sahul Hameed A. A new fibroblastic-like cell line from heart muscle of the Indian major carp(Catla catla):Development and characterization. Aquaculture 2009b, 293(3-4):180-186
104.Joerink M, Ribeiro CM, Stet, R J, Hermsen T, Savelkoul HF, Wiegertjes GF. Head kidney-derived macrophages of common carp (Cyprinus carpio L.) show plasticity and functional polarization upon differential stimulation. J Immunol 2006,177(1):61-69
105.Kang M, Oh M, Kim Y, Kawai K, Jung S. Establishment and characterization of two new cell lines derived from flounder, Paralichthys olivaceus (Temminck & Schlegel). J Fish Dis 2003,26(11-12):657-665
106.Katakura Y, Alam S, Shirahata S. Immortalization by gene transfection. Methods Cell Biol 1998,57:69-91
107.Kelly R, Miller H, Nielsen O, Clayton J. Fish cell culture:characteristics of a continuous fibroblastic cell line from Walleye (Stizostedion vitreum vitreum). Can J Fish Aquat Sci 1980,37,1070-1075
108.Kerstner-Wood C, Coward L, Gorman G. U.S. EPA docket AR-226-1354. Washington, DC:U.S. Environmental Protection Agency. Protein Binding of Perfluorobutane Sulfonate, Perfluorohexane Sulfonate, Perfluorooctane Sulfonate and Perfluorooctanoate to Plasma (Human, Rat and Monkey), and Various Human-Derived Plasma Protein Fractions.2003
109.Key BD, Howell RD, Criddle CS. Fluorinated organics in the biosphere. Environ Sci Technol 1997,31(9):2445-2454
110.Kiyono T, Foster SA, Koop JI, McDougall JK, Galloway DA, Klingelhutz AJ. Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 1998,396(6706):84-88
11 l.Komura JI, Mitani H, Shima A. Fish cell culture:Establishment of two fibroblast-like cell lines (OL-17 and OL-32) from fins of the medaka, Oryzias latipes. In Vitro Cell Dev-An 1988,24(4):294-298
112.Kondo H, Watabe S. Temperature-dependent enhancement of cell proliferation and mRNA expression for type I collagen and HSP70 in primary cultured goldfish cells. Comp Biochem Physiol A Mol Integr Physiol 2004,138(2):221-228
113.Ku CC, Teng YC, Wang CS, Lu CH. Establishment and characterization of three cell lines derived from the rockfish grouper Epinephelus quoyanus:Use for transgenic studies and cytotoxicity testing. Aquaculture 2009,294(1-2):147-151
114.Lai YS, John J, Lin CH, Guo IC, Chen SC, Fang K, Chang CY. Establishment of cell lines from a tropical grouper, Epinephelus awoara (Temminck & Schlegel), and their susceptibility to grouper irido-and nodaviruses. J Fish Dis 2003,26(1): 31-42
115.Lai YS, Murali S, Chiu HC, Ju HY, Lin Y S, Chen SC, Guo IC, Fang K, Chang CY. Propagation of yellow grouper nervous necrosis virus (YGNNV) in a new nodavirus-susceptible cell line from yellow grouper, Epinephelus awoara (Temminck & Schlegel), brain tissue. JFish Dis 2001,24(5):299-309
116.Lai YS, Murali S, Ju HY, Wu MF, Guo IC, Chen SC, Fang K, Chang CY. Two iridovirus-susceptible cell lines established from kidney and liver of grouper, Epinephelus awoara (Temminck & Schlegel), and partial characterization of grouper iridovirus. J Fish Dis 2000,23(6):379-388
117.Lakra WS, Swaminathan TR, Rathore G, Goswami M, Yadav K, Kapoor S. Development and characterization of three new diploid cell lines from Labeo rohita (Ham.). Biotechnol Prog 2010,26(4):1008-1013
118.Lakra W, Swaminathan TR, Joy K. Development, characterization, conservation and storage of fish cell lines:a review. Fish Physiol Biochem 2011,37(1):1-20
119.Lakra WS, Sivakumar N, Goswami M, Bhonde RR. Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch). Aquac Res 2006, 37(1):18-24
120.Lamche G, Meier W, Suter M, Burkhardt-Holm P. Primary culture of dispersed skin epidermal cells of rainbow trout Oncorhynchus mykiss Walbaum. Cell Mol Life Sci 1998,54(9):1042-1051
121.Lau C, Anitole K, Hodes C, Lai D, Pfahles-Hutchens A, Seed J. Perfluoroalkyl acids:a review of monitoring and toxicological findings. Toxicol Sci 2007,99(2): 366-394
122.Leguen I, Peron S, Prunet P. Effects of iron on rainbow trout gill cells in primary culture. Cell Biol Toxicol 2011,27(5):311-319
123.Lein NPH, Fujii S, Tanaka S, Nozoe M, Tanaka H. Contamination of perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) in surface water of the Yodo River basin (Japan). Desalination 2008,226(1-3):338-347
124.Levan A, Fredga K, Sandberg AA. Nomenclature for centromeric position on chromosomes. Hereditas 1964,52(2):201-220
125.Lewis D, Marks J. Microcultures of Sarotherodon mossambicus (Peters) cells: Their use in detecting fish viruses. J Fish Dis 1985,8(5):477-478
126.Li SF, Cai WQ. Genetic improvement of the herbivorous blunt snout bream (Megalobrama amblycephala). Naga 2003,26 (1):20-23
127.Lu RH, Liang XF, Wang M, Zhou Y, Bai XL, He Y The role of leptin in lipid metabolism in fatty degenerated hepatocytes of the grass carp Ctenopharyngodon idellus. Fish Physiol Biochem 2012,38(6):1759-1774
128.Lu Y, Aguirre AA, Hamm C, Wang Y, Yu Q, Loh PC, Yanagihara R. Establishment, cryopreservation, and growth of 11 cell lines prepared from a juvenile Hawaiian monk seal, Monachus schauinslandi. Methods Cell Sci 2000, 22(2-3):115-124
129.Luebker DJ, Hansen KJ, Bass NM, Butenhoff JL, Seacat AM. Interactions of flurochemicals with rat liver fatty acid-binding protein. Toxicology 2002,176(3): 175-185
130.Luo W, Zeng C, Deng W, Robinson N, Wang WM, Gao ZX. Genetic parameter estimates for growth-related traits of blunt snout bream(Megalobrama amblycephala) using microsatellite-based pedigree. Aquac Res 2013,1-8, DOI 10.1111/are.12133
131.Matsumoto J, Ishikawa T, Masahito P, Takayama S. Permanent cell lines from erythrophoromas in goldfish(Carassius auratus). JNatl Cancer Inst 1980,64(4): 879-890
132.Matsuo Y, Nishizaki C, Drexler H. Efficient DNA fingerprinting method for the identification of cross-culture contamination of cell lines. Hum Cell 1999,12(3): 149-154
133.Mauger PE, Labbe C, Bobe J, Caury C, Leguen I, Baffet G, Le Bail PY. Characterization of goldfish fin cells in culture:some evidence of an epithelial cell profile. Comp Biochem Physiol B Biochem Mol Biol 2009,152(3):205-215
134.Miller NW, Sizemore R, Clem L. Phylogeny of lymphocyte heterogeneity:the cellular requirements for in vitro antibody responses of channel catfish leukocytes. J Immunol 1985,134(5):2884-2888
135.Mitani H, Naruse K, Shima A. Eurythermic and stenothermic growth of cultured fish cells and their thermosensitivity. J Cell Sci 1989,93(Pt4):731-737
136.Moody CA, Martin JW, Kwan WC, Muir DC, Mabury SA. Monitoring perfluorinated surfactants in biota and surface water samples following an accidental release of fire-fighting foam into Etobicoke Creek. Environ Sci Technol 2002,36(4):545-551
137.Morikawa A, Kamei N, Harada K, Inoue K, Yoshinaga T, Saito N, Koizumi A. The bioconcentration factor of perfluorooctane sulfonate is significantly larger than that of perfluorooctanoate in wild turtles (Trachemys scripta elegans and Chinemys reevesii):an Ai river ecological study in Japan. Ecotoxicol Environ Saf 2006,65(1):14-21
138.Moritz C, Labbe C. Cryopreservation of goldfish fins and optimization for field scale cryobanking. Cryobiology 2008,56(3):181-188
139.Mothersill C, Lyng F, Lyons M, Cottell D. Growth and differentiation of epidermal cells from the rainbow trout established as explants and maintained in various media. JFish Biol 1995,46(6):1011-1025
140.Mulkiewicz E, Jastorff B, Skladanowski A, Kleszczynski K, Stepnowski P. Evaluation of the acute toxicity of perfluorinated carboxylic acids using eukaryotic cell lines, bacteria and enzymatic assays. Environ Toxicol Pharmacol 2007,23(3):279-285
141.Nicholson BL, Byrne C. An established cell line from the Atlantic salmon(Salmo salar). JFish Board Can 1973,30(70):913-916
142.Nicholson B, Danner D, Wu JL. Three new continuous cell lines from marine fishes of Asia. In Vitro Cell Dev-An 1987,23(3):199-204
143.Olsen GW, Gilliland FD, Burlew MM, Burris JM, Mandel JS, Mandel JH. An epidemiologic investigation of reproductive hormones in men with occupational exposure to perfluorooctanoic acid. J Occup Environ Med 1998,40(7):614-622
144.Organisation for Economic Cooperation and Development (OECD). Hazard Assessment of Perfluorooctane Sulfonate (PFOS) and Its Salts. US EPA docket AR-226-1140.2002
145.0u-yang L, Huang XH, Huang EY, Huang YH, Gong J, Sun JJ, Qin QW. Establishment and characterization of a new marine fish cell line derived from red-spotted grouper Epinephelus akaara. J Fish Biol 2010,77(5):1083-1095
146.Parameswaran V, Shukla R, Bhonde R, Hameed AS. Development of a pluripotent ES-like cell line from Asian sea bass(Lates calcarifer)-an oviparous stem cell line mimicking viviparous ES cells. Mar Biotechnol 2007,9(6):766-775
147.Parameswaran V, Shukla R, Bhonde R, Sahul Hameed A. Splenic cell line from sea bass, Lates calcarifer:Establishment and characterization. Aquaculture 2006, 261(1):43-53
148.Pastoor TP, Lee KP, Perri MA, Gillies PJ. Biochemical and morphological studies of ammonium perfluorooctanoate-induced hepatomegaly and peroxisome proliferation. Exp Mol Pathol 1987,47(1):98-109
149.Perry G, McDonald G, Ferguson M, Ganassin R, Bols N. Characterization of rainbow trout cell lines using microsatellite DNA profiling. Cytotechnology 2001, 37(3):143-151
150.Pettersen EF, Ingerslev HC, Stavang V, Egenberg M, Wergeland HI. A highly phagocytic cell line TO from Atlantic salmon is CD83 positive and M-CSFR negative, indicating a dendritic-like cell type. Fish Shellfish Immunol 2008,25(6): 809-819
151.Phelan PE, Pressley ME, Witten PE, Mellon MT, Blake S, Kim CH. Characterization of snakehead rhabdovirus infection in zebrafish (Danio rerio). J Virol 2005,79(3):1842-1852
152.Pombinho AR, Laize V, Molha DM, Marques SM, Cancela ML. Development of two bone-derived cell lines from the marine teleost Sparus aurata; evidence for extracellular matrix mineralization and cell-type-specific expression of matrix Gla protein and osteocalcin. Cell Tissue Res 2004,315(3):393-406
153.Prasanna I, Lakra W, Ogale S, Bhonde R. Cell culture from fin explant of endangered golden mahseer, Tor putitora (Hamilton). Curr Sci India 2000,79(1): 93-94
154.Qin Q, Wu T, Jia T, Hegde A, Zhang R. Development and characterization of a new tropical marine fish cell line from grouper, Epinephelus coioides susceptible to iridovirus and nodavirus. J Virol Methods 2006,131(1):58-64
155.Ribeiro L, Ahne W. Fish cell culture:Initiation of a line of pituitary cells from carp(Cyprinus carpio) to study the release of gonadotropin in vitro. In vitro 1982, 18(5):419-420
156.Rougee L, Ostrander GK, Richmond RH, Lu Y. Establishment, characterization, and viral susceptibility of two cell lines derived from goldfish Carassius auratus muscle and swim bladder. Diseases Aquatic Organ 2007,77(2):127-135
157.Ruiz S, Schyth BD, Encinas P, Tafalla C, Estepa A, Lorenzen N, Coll J. New tools to study RNA interference to fish viruses:Fish cell lines permanently expressing siRNAs targeting the viral polymerase of viral hemorrhagic septicemia virus. Antiviral Res 2009,82(3):148-156
158.Sahul Hameed A, Parameswaran V, Shukla R, Bright Singh I, Thirunavukkarasu A, Bhonde R. Establishment and characterization of India's first marine fish cell line (SISK) from the kidney of sea bass(Lates calcarifer). Aquaculture 2006, 257(1-4):92-103
159.Santos Y, Pazos F, Bandin I, Toranzo AE. Analysis of antigens present in the extracellular products and cell surface of Vibrio anguillarum serotypes 01,02, and 03. Appl Environ Microbiol 1995,61(7):2493-2498
160.Santos-Ruiz L, Santamaria J, Becerra J. Cytoskeletal dynamics of the teleostean fin ray during fin epimorphic regeneration. Differentiation 2005,73(4):175-187
161.Schaffeld M, Markl J. Fish keratins. Methods Cell Biol 2004,78,627-671
162.Shen L, Stuge TB, Zhou H, Khayat M, Barker KS, Quiniou S, Wilson M, Bengten E, Chinchar VG, Clem LW. Channel catfish cytotoxic cells:a mini-review. Dev Comp Immunol 2002,26(2):141-149
163.Shima A, Nikaido O, Shinohara S, Egami N. Continued in vitro growth of fibroblast-like cells (RBCF-1) derived from the caudal fin of the fish, Carassius auratus. Exp Gerontol 1980,15(4):305-314
164.Skutlarek D, Exner M, Farber H. Perfluorinated surfactants in surface and drinking waters. Environ Sci Pollut Res Int 2006,13(5):299-307
165.Sobhana K, George K, Venkat Ravi G, Ittoop G, Paulraj R. Development of a cell culture system from gill explants of the grouper, Epinephelus malabaricus (Bloch and Shneider). Asian Fish Sci 2009,22(2):541-547
166.Sohlenius AK, Andersson K, DePierre JW. The effects of perfluoro-octanoic acid on hepatic peroxisome proliferation and related parameters show no sex-related differences in mice. Biochem J 1992,285(Pt 3):779-783
167.Sohlenius AK, Eriksson AM, Hogstrom C, Kimland M, DePierre JW. Perfluorooctane sulfonic acid is a potent inducer of peroxisomal fatty acid β-oxidation and other activities known to be affected by peroxisome proliferators in mouse liver. Pharmacol Toxicol 1993,72(2):90-93
168.Somamoto T, Nakanishi T, Okamoto N. Specific cell-mediated cytotoxicity against a virus-infected syngeneic cell line in isogeneic ginbuna crucian carp. Dev Comp Immunol 2000,24(6-7):633-640
169. Song Y, Liu L, Shen H, You J, Luo Y. Effect of sodium alginate-based edible coating containing different anti-oxidants on quality and shelf life of refrigerated bream (Megalobrama amblycephala). Food Control 2011,22(3-4):608-615
170.Starkov AA, Wallace KB. Structural determinants of fluorochemical-induced mitochondrial dysfunction. Toxicol Sci 2002.66(2):244-252.
171.Stone D, Ahne W, Denham K, Dixon P, Liu C, Sheppard A, Taylor G, Way K. Nucleotide sequence analysis of the glycoprotein gene of putative spring viraemia of carp virus and pike fry rhabdovirus isolates reveals four genogroups. Dis Aquat Organ 2003,53(3):203-210
172.Swaminathan TR, Lakra WS, Gopalakrishnan A, Basheer V, Khushwaha B, Sajeela K. Development and characterization of a new epithelial cell line PSF from caudal fin of Green chromide, Etroplus suratensis (Bloch,1790). In Vitro Cell Dev Biol Anim 2010,46(8):647-656
173.Tan F, Wang M, Wang W, Lu Y. Comparative evaluation of the cytotoxicity sensitivity of six fish cell lines to four heavy metals in vitro. Toxicol In Vitro 2008, 22(1):164-170
174.Tong SL, Li H, Miao HZ. The establishment and partial characterization of a continuous fish cell line FG-9307 from the gill of flounder Paralichthys olivaceus. Aquaculture 1997,156(3-4):327-333
175.USEPA. Preliminary Risk Assessment of the Developmental Toxicity Associated with Exposure to Perfluorooctanoic Acid and Its Salts.2003
176.Vaziri H, Benchimol S. Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span. Curr Biol 1998,8(5):279-282
177.Wagg SK, Lee LE. A proteomics approach to identifying fish cell lines. Proteomics 2005,5(16):4236-4244
178.Wang G, Lapatra S, Zeng L, Zhao Z, Lu Y. Establishment, growth, cryopreservation and species of origin identification of three cell lines from white sturgeon, Acipenser transmontanus. Meth Cell Sci 2004,25(3-4):211-220
179. Wang N, Wang X, Sha Z, Tian Y, Chen S. Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus). Fish Physiol Biochem 2010a,36(4):1227-1234
180.Wang X, Wang N, Sha Z, Chen S. Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis). Fish Physiol Biochem 2010b,36(4):1181-1189
181.Wei YB, Fan TJ, Jiang GJ, Sun A, Xu XH, Wang J. Establishment of a novel fin cell line from Brown-marbled grouper, Epinephelus fuscoguttatus (Forsskal), and evaluation of its viral susceptibility. Aquac Res 2009,40(13):1523-1531
182. Wei Y, Liu Y, Wang J, Tao Y, Dai J. Toxicogenomic analysis of the hepatic effects of perfluorooctanoic acid on rare minnows (Gobiocypris rarus). Toxicol Appl Pharmacol 2008,226(3):285-297
183.White SS, Calafat AM, Kuklenyik Z, Villanueva L, Zehr RD, Helfant L, Strynar MJ, Lindstrom AB, Thibodeaux JR, Wood C. Gestational PFOA exposure of mice is associated with altered mammary gland development in dams and female offspring. Toxicol Sci 2007,96(1):133-144
184.Wolf K, Mann JA. Poikilotherm vertebrate cell lines and viruses:a current listing for fishes. In Vitro 1980,16(2):168-179
185.Wolf K, Quimby M. Established eurythermic line of fish cells in vitro. Science 1962,135(3508):1065-1066
186.Wondergem R, Amsler K. Transmembrane protentials of cultured rectal gland cells of Raja erinacea. Bull Mt Desert Is1 Bio Lab 1986,26:105-107
187.Wu Y, Chi S. Persistence of betanodavirus in barramundi brain (BB) cell line involves the induction of interferon response. Fish Shellfish Immunol 2006,21(5): 540-547
188.Xiao Q, Li D, Liu H. A flounder (Paralichthys olivaceus) gill cell line as in vitro acute assay system of nonylphenol cytotoxicity. Environ Monit Assess 2011, 175(1-4):315-319.
189.Xing JQ El-Sweisi W, Lee L, Collodi P, Seymour C, Mothersill C, Bols NC. Development of a zebrafish spleen cell line, ZSSJ, and its growth arrest by gamma irradiation and capacity to act as feeder cells. In Vitro Cell Dev Biol Anim 2009,45(3-4):163-174
190.Yan W, Nie P, Lu Y. Establishment, characterization and viral susceptibility of a new cell line derived from goldfish, Carassius auratus (L.), tail fin. J Fish Dis 2011,34(10):757-768
191.Ye HQ, Chen SL, Sha ZX, Xu MY. Development and characterization of cell lines from heart, liver, spleen and head kidney of sea perch Lateolabrax japonicus. J Fish Biol 2006,69(sa):115-126
192.Yi M, Hong N, Hong Y. Generation of medaka fish haploid embryonic stem cells. Science 2009,326(5951):430-433
193.Zhang B, Wang X, Sha Z, Yang C, Liu S, Wang N, Chen SL. Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis. IntJBiol Sci 2011,7(4):452-459
194.Zhang L, Luo Q, Fang Q, Wang Y. An improved RT-PCR assay for rapid and sensitive detection of grass carp reovirus. J Virol Methods 2010,169(1):28-33
195.Zhang X, Xie P, Zhang X, Zhou W, Zhao S, Zhao Y, Cai Y. Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions. J Appl Toxicol 2012, DOI 10.1002/jat.2749
196.Zhao Y, Gul Y, Li S, Wang W. Cloning, identification and accurate normalization expression analysis of PPARa gene by GeNorm in Megalobrama amblycephala. Fish Shellfish Immunol 2011,31(3):462-468
197.Zhao Z, Lu Y. Establishment and characterization of two cell lines from bluefin trevally Caranx melampygus. DisAquat Organ 2006,68(2):91-100
198.Zhao Z, Montgomery-Brock D, Lee CS, Lu Y. Establishment, characterization and viral susceptibility of 3 new cell lines from snakehead, Channa striatus (Blooch). Methods Cell Sci 2004,25(3-4):155-166
199.Zhou GZ, Li ZQ, Yuan XP, Zhang QY. Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara). Mar Biotechnol 2007,9(3):370-376
200.Zhou G, Gui L, Li Z, Yuan X, Zhang Q. Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus. J Fish Biol 2008,73(8):2058-2067
201.Zhou L, Deane E, Woo N. Development of a black sea bream fibroblast cell line and its potential use as an in vitro model for stress protein studies. Fish Physiol Biochem 2003,29(4):255-262
202.Zhu DM, Yang K, Wang WM, Song W. Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala. Fish Physiol Biochem 2013,1-12. DOI 10.1007/s10695-013-9794-6
203.Zou S, Li S, Cai W, Zhao J, Yang H. Establishment of fertile tetraploid population of blunt snout bream (Megalobrama amblycephala). Aquaculture 2004,238(1-4): 155-164.
2.崔燕,白承连,徐涛,王晓彤,陈元红,靳大庆.PFOA对斑马鱼胚胎发育、行为和DNA损伤的毒性研究(英文).生态毒理学报,2012,7(3)：241-250
3.杜玉东,陈孝煊,吴志新,王敏,刘迁,夏君,王树云,李莉娟.斑点叉尾鮰疱疹病毒PCR-ELISA检测方法的建立.华中农业大学学报,2009,28(5)：604-608
4.弗雷谢尼RI著.动物细胞培养：基本技术指南.章静波,徐存拴译.5版.北京：科学出版社,2008.30-90
5.霍静,洪锡钧,王志坚,刘小红,王咏梅.南方鲇肾细胞系(SMK-I)的建立及其细胞骨架的研究.西南大学学报(自然科学版),2007,29(12)：128-133
6.金一和,丁梅,翟成,王烈,董光辉,舒为群,张颖花,齐藤宪光,佐佐木和明.长江三峡库区江水和武汉地区地面水中PFOS和PFOA污染现状调查.生态环境,2006,15(3)：486-489
7.金一和,刘晓,李彤,秦红梅,张颖花,齐藤宪光,佐佐木和明,小泉昭夫.沈阳地区成人血清和脐带血中全氟有机物污染现状.卫生研究,2004,33(4)：481-483
8.孔祥婷,沈颂东,姜德勋,姜红霞,徐健荣,张君,韩晓磊,许璞.黄颡鱼吻端细胞培养研究.淡水渔业,2009,39(2)：19-25
9.李思发,蔡完其.团头鲂双向选育效应研究.水产学报,2000,24(3)：201-205
10.李思发,杨学明.双向选择对团头鲂生化遗传变异的影响.中国水产科学,1996,3(1)：1-5
11.李文峰,麦康森,黄倢,史成银.鱼类细胞培养及其在病毒学研究中的应用.动物医学进展,2010,35(1)：107-110
12.李杨.团头鲂三个野生群体的遗传结构分析及遗传图谱的构建.[博士学位论文].武汉：华中农业大学图书馆,2010
13.刘肖莲.鳜鱼与团头鲂微卫星标记的应用及分子育种基础研究.[博士学位论文].武汉：华中农业大学图书馆,2012
14.刘新建,李贵生.国内鱼类病毒病研究进展.生态科学,2004,23(3)：282-285
15.卢向明,陈萍萍.低剂量全氟辛酸对雄性黑斑蛙生殖毒效应及机理研究.环境科学学报,2012,32(6)：1497-1502
16.冉玮,张桂蓉,王卫民,魏开建,周玲玲.利用SRAP标记分析3个团头鲂群体的遗传多样性.华中农业大学学报,2010,29(5)：601-606.
17.任秉新.大菱鲆(Scophthalmus maximus)肝细胞体外培养、红体病虹彩病毒体外繁殖条件及病毒疫苗研制的研究.[博士学位论文].青岛：中国海洋大学图书馆,2009
18.任国诚,陈松林,沙珍霞.半滑舌鳎肝脏细胞系的建立与鉴定.高技术通讯,2008,18(6)：657-660
19.沈锦玉.嗜水气单胞菌外膜提取物及胞外产物特性的分析.[硕士学位论文].杭州：浙江大学图书馆,2002
20.谭凤霞,王敏,王卫民.利用草鱼CIK细胞和MTT法测定镉和铬毒性试验的优化.水生生物学报,2006,30(3)：371-374
21.谭凤霞,杨方星,王卫民,王敏.稀有鮈鲫鳍细胞系的建立及其作为测定重金属毒性模型的探讨.水生生物学报,2009,33(4)：767-771
22.谭凤霞.三株鱼类细胞系的建立和十二株鱼类细胞系对重金属毒性的敏感性研究.[博士学位论文].武汉：华中农业大学图书馆,2008
23.唐首杰,李思发,蔡完其.团头鲂野生、驯养、选育3类遗传生态群体遗传变异的线粒体DNA分析.中国水产科学,2011,18(3)：483-492
24.王京真,姬伟,肖武汉,王丁.长江江豚脐静脉细胞体外培养的初步研究.水生生物学报,2010,34(2)：436-441
25.王铁辉,张义兵,李戈强,易泳兰,刘汉勤,朱作言.鱼类培养细胞干扰素的诱导.病毒学报,1999,15(1)：47-53
26.王贤丽.几种重要海水养殖鱼类细胞系的建立、诱导分化及其应用研究.[博士学位论文].青岛：中国海洋大学图书馆,2009
27.韦新兰,张杰,陈丽萍,王卫民,高泽霞,王焕岭.团头鲂SPATA4基因的 分子克隆及表达分析.华中农业大学学报,2012,31(6)：737-743
28.魏彦章,陆仁后,白国栋.草鱼尾鳍组织二倍体细胞系GCCF-2的建立及其部分生物学特性分析.水产学报,1987,11(4)：269-275
29.魏云波,刘书花,王兴民,周延生,孙爱.石鲽鳍细胞系的建立及三丁基锡对其毒性作用的研究.现代农业科技,2011,19：13-14,18
30.魏云波.褐点石斑鱼(Epinephelus fuscoguttatus)三种细胞系的建立、病毒敏感性以及环境污染物对其毒性作用的研究.[博士学位论文].青岛：中国海洋大学图书馆,2009
31.谢保胜,王刚,史健全,祁洪芳.青海湖裸鲤体外肝胰细胞原代与传代培养.生物学杂志,2009,26(4)：34-37
32.徐晓辉.两种星鲽组织细胞系的建立及久效磷对其毒性作用的研究.[硕士学位论文].青岛：中国海洋大学图书馆,2010
33.杨谨,李昂,杨谦,李旭.过氧化物酶体增殖剂PFOA对小鼠免疫系统的影响.细胞与分子免疫学杂志,2006,22(2)：157-160
34.杨先乐.鱼类组织培养的回顾与展望.水产学报,1999,23：74-81
35.姚晓峰,仲来福.全氟辛酸对HepG2细胞的遗传毒性及氧化性DNA损伤.毒理学杂志,2005,19(3)：216-217
36.姚晓峰.全氟辛酸对HepG2细胞的遗传毒性及氧化性DNA损伤.[硕士学位论文].大连：大连医科大学.,2006
37.叶露,吴玲玲,蒋雨希,张超杰,陈玲.PFOS/PFOA对斑马鱼(Danio rerio)胚胎致毒效应研究.环境科学,2009,30(6)：1727-1732
38.叶露.典型全氟化合物水生毒理学效应与致毒机理初探.[硕士学位论文].上海：同济大学图书馆,2008
39.尹洪滨,范兆廷,孙中武,潘峰,孟繁荣.团头鲂核型与DNA含量分析研究.水产学杂志,1995,8(1)：22-26
40.于淼,管华诗,郭华荣,张士璀.鱼类细胞培养及其应用.海洋科学,2003,27(3)：4-8
41.曾聪,阎里清,高泽霞,曹小娟,钱雪桥,王卫民.梁子湖、鄱阳湖和淤泥湖团头鲂的形态学比较.华中农业大学学报,2012,31(1)：88-94
42.曾令兵,李晓莉,张林,徐进,张燕.斑点叉尾鮰肾脏组织细胞系的建立及 其生物学特性.中国水产科学,2009,16(1)：75-81
43.张贵生,傅荣恕.稀土与铜对鲹鲦鱼红细胞微核及核异常的影响.四川动物,2007,26(3)：644-646
44.张念慈,杨广智.草鱼吻端组织细胞株ZC-7901及其亚株ZC-7901S1的建立和特性观察.水产学报,1981,5(1)：111-120
45.张奇亚.我国水生动物病毒病研究概况.水生生物学报,2002,26(1)：89-101
46.张倩,张超杰,周琪,陈玲. SPE-HPLC/MS联用法测定地表水中的PFOA及PFOS含量.四川环境,2006,25(4)：10-12,28
47.张新辉,夏新民,罗伟,高泽霞,钱雪桥,王卫民.团头鲂雌核发育后代的微卫星标记分析.华中农业大学学报,2012,31(6)：737-743
48.张新辉.团头鲂三倍体和雌核发育的诱导及其主要生物学特性的研究.[硕士学位论文].武汉：华中农业大学图书馆,2013
49.张义兵,俞小牧,陈敏容,赵庆中,陈道权,王铁辉,王丁.白鱀豚组织培养的初步研究.水生生物学报,2000,24(2)：193-195
50.中国渔业统计年鉴,北京：中国农业出版社,2001
51.左文功,钱华鑫,许映芳,杜森英,杨先乐.草鱼肾组织细胞系CIK的建立及其生物学特性.水产学报,1986,10(1)：11-17
52. Akimoto K, Takaoka T, Sorimachi K. Development of a simple culture method for the tissues contaminated with microorganisms and application to establishment of a fish cell line. Zool Sci 2000,17(1):61-63
53. Ariel E, Nicolajsen N, Christophersen M B, Holopainen R, Tapiovaara H, Bang Jensen B. Propagation and isolation of ranaviruses in cell culture. Aquaculture 2009,294(3-4):159-164
54. Aviv A. How long should telomeres be? Current hypertension reports 2001,3(2): 145-151.
55. Babu V, Nambi K, Chandra V, Ishaq Ahmed V, Bhonde R, Sahul Hameed A. Establishment and characterization of a fin cell line from Indian walking catfish, Clarias batrachus (L.). JFish Dis 2011,34(5):355-364
56. Balebona M, Krovacek K, Morinigo M, Mansson I, Faris A, Borrego J. Neurotoxic effect on two fish species and a PC12 cell line of the supernate of Vibrio alginolyticus and Vibrio anguillarum. Vet Microbiol 1998,63:61-69
57. Barker C, Rackham B. The induction of sister-chromatid exchanges in cultured fish cells (Ameca splendens) by carcinogenic mutagens. Mutat Res 1979,68(4): 381-387
58. Bayne BL, Brown DA, Burns K, Dixon DR, Ivanovici A, Livingstone DA, Lowe DM, Moore MN, Stebbing ARD, Widdings J. The effects of stress and pollution on marine animals. Praeger, New York, USA.1985
59. Bejar J, Borrego JJ, Alvarez MC. A continuous cell line from the cultured marine fish gilt-head seabream (Sparus aurata L.). Aquaculture 1997,150(1-2):143-153
60. Berthiaume J, Wallace KB. Perfluorooctanoate, perflourooctanesulfonate, and N-ethyl perfluorooctanesulfonamido ethanol; peroxisome proliferation and mitochondrial biogenesis. Toxicol Lett 2002,129(1-2):23-32
61. Beyenbach KW, Booz G, Kleinzeller A. Transepithelial voltages and resistances across isolated perfused renal tubules of the winter flounder. Bull Mt Desert Isl Biol Lab 1980,20:78-82
62. Biegel LB, Hurtt ME, Frame SR, O'Connor JC, Cook JC. Mechanisms of extrahepatic tumor induction by peroxisome proliferators in male CD rats. Toxicol Sci 2001,60(1):44-55
63. Bodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu CP, Morin GB, Harley CB, Shay JW, Lichtsteiner S, Wright WE. Extension of life-span by introduction of telomerase into normal human cells. Science 1998,279(5349):349-352
64. Bols N, Dayeh V, Lee L, Schirmer K. Use of fish cell lines in the toxicology and ecotoxicology of fish. Piscine cell lines in environmental toxicology. Biochem Mol Biol Fish 2005,6:43-84
65. Boulanger B, Vargo J, Schnoor JL, Hornbuckle KC. Detection of perfluorooctane surfactants in Great Lakes water. Environ Sci Technol 2004,38(15):4064-4070
66. Bowser PR, Plumb JA. Fish cell lines:establishment of a line from ovaries of channel catfish. In vitro 1980,16(5):365-368
67. Chen MJ, Chiou PP, Liao YH, Lin CM, Chen TT. Development and characterization of five rainbow trout pituitary single-cell clone lines capable of producing pituitary hormones. JEndocrinol 2010,205(1):69-78
68. Chen QL, Luo, Z, Liu X, Song YF, Liu CX, Zheng JL, Zhao YH. Effects of waterborne chronic copper exposure on hepatic lipid metabolism and metal-element composition in synechogobius hasta. Arch Environ Contam Toxicol 2013, 64(2):301-315
69. Chen SL, Ren GC, Sha ZX, Hong Y. Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus). Aquaculture 2005,249(1-4):63-68
70. Chen SL, Ren GC, Sha ZX, Hong Y. Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus). Aquaculture 2005,249(1-4):63-68
71. Chen SL, Ren GC, Sha ZX, Shi CY. Establishment of a continuous embryonic cell line from Japanese flounder Paralichthys olivaceus for virus isolation. Dis Aquat Org 2004,60:241-246
72. Chen SL, Sha ZX, Ye H Q. Establishment of a pluripotent embryonic cell line from sea perch(Lateolabrax japonicus) embryos. Aquaculture 2003,218(1-4): 141-151
73. Cheng LL, Bowser P, Spitsbergen J. Development of cell cultures derived from lake trout liver and kidney in a hormone-supplemented, serum-reduced medium. J Aquat Anim Health 1993,5:119-126
74. Chi S, Hu W, Lo B. Establishment and characterization of a continuous cell line (GF-1) derived from grouper, Epinephelus coioides (Hamilton):a cell line susceptible to grouper nervous necrosis virus (GNNV). J Fish Dis 1999,22(3): 173-182
75. Clem LW, Moewus L, Sigel MM. Studies with cells from marine fish in tissue culture, Proceedings of the society for experimental biology and medicine. Society for experimental biology and medicine (New York, NY). Royal Society of Medicine 1961,762-766
76. Collodi P, Kame Y, Ernst T, Miranda C, Buhler DR, Barnes DW. Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues. Cell Biol Toxicol 1992,8(1):43-61
77. Costa-Ramos C, Rowley AF. Effect of extracellular products of Pseudoalteromonas atlantica on the edible crab Cancer pagurus. Appl Environ Microbiol 2004,70(2):729-735
78. Cuchens MA, Clem LW. Phylogeny of lymphocyte heterogeneity:II. Differential effects of temperature on fish T-like and B-like cells. Cell Immunol 1977,34(2): 219-230
79. DeWitte-Orr S, Lepic K, Bryson S, Walsh S, Lee L, Bols N. Development of a continuous cell line, PBLE, from an American eel peripheral blood leukocyte preparation. In Vitro Cell Dev Biol Anim 2006,42(8-9):263-272
80. Dinglasan MJA, Ye Y, Edwards EA, Mabury SA. Fluorotelomer alcohol biodegradation yields poly-and perfluorinated acids. Environ Sci Technol 2004, 38(10):2857-2864
81. Dong C, Weng S, Shi X, Xu X, Shi N, He J. Development of a mandarin fish Siniperca chuatsi fry cell line suitable for the study of infectious spleen and kidney necrosis virus (ISKNV). Virus Res 2008,135(2):273-281
82. Ellis DA, Martin JW, De Silva AO, Mabury SA, Hurley MD, Sulbaek Andersen MP, Wallington TJ. Degradation of fluorotelomer alcohols:a likely atmospheric source of perfluorinated carboxylic acids. Environ Sci Technol 2004,38(12): 3316-3321
83. Fan L, Collodi P. Progress towards cell-mediated gene transfer in zebrafish. Briefings Funct Genomic Proteomic 2002,1(2):131-138
84. Fan TJ, Ren BX, Geng XF, Yu QT, Wang LY. Establishment of a turbot fin cell line and its susceptibility to turbot reddish body iridovirus. Cytotechnology 2010, 62(3):217-223
85. Fijan N, Sulimanovic D, Bearzotti M, Muzinic D, Zwillenberg L, Chilmonczyk S, Vautherot J, De Kinkelin P. Some properties of the Epithelioma papulosum cyprini (EPC) cell line from carp cyprinus carpio. Annales De l'Institut Pasteur-/Virologie 1983,134(2):207-220
86. Forest D, Nishikawa R, Kobayashi H, Parton A, Bayne C J, Barnes D W. RNA expression in a cartilaginous fish cell line reveals ancient 3'noncoding regions highly conserved in vertebrates. Proceedings of the National Academy of Sciences 2007,104(4):1224-1229
87. Frerichs G, Morgan D, Hart D, Skerrow C, Roberts R, Onions D. Spontaneously productive C-type retro virus infection of fish cell lines. J Gen Virol 1991,72(10): 2537-2539
88. Fryer JL, Lannan C. Three decades of fish cell culture:a current listing of cell lines derived from fishes. J Tissue Cult Meth 1994,16(2):87-94
89. Garcia DM, Bauer H, Dietz T, Schubert T, Markl J, Schaffeld M. Identification of keratins and analysis of their expression in carp and goldfish:comparison with the zebrafish and trout keratin catalog. Cell Tissue Res 2005,322(2):245-256
90. Goecke-Flora CM, Reo NV. Influence of carbon chain length on the hepatic effects of perfluorinated fatty acids. Chem Res Toxicol 1996,9(4):689-695
91. Gravell M, Malsberger R. A permanent cell line from the fathead minnow (Pimephales promelas).Ann N Y Acad Sci 1965,126(1):555-565
92. Guruge KS, Yeung LW, Yamanaka N, Miyazaki S, Lam PK, Giesy JP, Jones PD, Yamashita N. Gene expression profiles in rat liver treated with perfluoroctanoic acid (PFOA). Toxicol Sci 2006,89(1):93-107
93. Han X, Kcrmpcr RA, Jepson GW. Subcellular Distribution and protein binding of pcrfluorooctanoic acid in rat liver and kindey. Drug Chem Toxicol 2005,28(2): 197-209
94. Hashimoto H, Toyohara H, Yokoyama Y, Sakaguchi M, Ozato K, Wakamatsu Y. Effects of carp serum on the growth of goldfish fin cells in early passage. J Fish Biol 1997,50(1):201-207
95. Haughom B, Spydevold 0. The mechanism underlying the hypolipemic effect of perfluorooctanoic acid (PFOA), perfluorooctane sulphonic acid (PFOSA) and clofibric acid. Biochim BiophysActa 1992,1128(1):65-72
96. Herrmann H, Munick M, Brettel M, Fouquet B, Markl J. Vimentin in a cold-water fish, the rainbow trout:highly conserved primary structure but unique assembly properties. J cell Sci 1996,109(3):569-578
97. Hightower LE, Renfro JL. Recent applications of fish cell culture to biomedical research. JExp Zool 1988,248(3):290-302
98. Holen E, Kausland A, Skjaerven K. Embryonic stem cells isolated from Atlantic cod (Gadus morhua) and the developmental expression of a stage-specific transcription factor ac-Pou2. Fish Physio Biochem 2010,36(4):1029-1039
99. Huang X, Huang Y, Ouyang Z, Qin Q. Establishment of a cell line from the brain of grouper(Epinephelus akaara) for cytotoxicity testing and virus pathogenesis. Aquaculture 2011,311(1-4):65-73
100.Huang X, Huang Y, Sun J, Han X, Qin Q. Characterization of two grouper Epinephelus akaara cell lines:Application to studies of Singapore grouper iridovirus (SGIV) propagation and virus-host interaction. Aquaculture 2009, 292(3-4):172-179
101.Ishaq Ahmed V, Chandra V, Parameswaran V, Venkatesan C, Shukla R, Bhonde R, Sahul Hameed A. A new epithelial-like cell line from eye muscle of catla Catla catla (Hamilton):development and characterization. J Fish Biol 2008,72(8): 2026-2038
102.Ishaq Ahmed V, Chandra V, Sudhakaran R, Rajesh Kumar S, Sarathi M, Sarath Babu V, Ramesh B, Sahul Hameed A. Development and characterization of cell lines derived from rohu, Labeo rohita (Hamilton), and catla, Catla catla (Hamilton). JFish Dis 2009a,32(3):211-218
103.Ishaq Ahmed V, Sarath Babu V, Chandra V, Nambi K, Thomas J, Bhonde R, Sahul Hameed A. A new fibroblastic-like cell line from heart muscle of the Indian major carp(Catla catla):Development and characterization. Aquaculture 2009b, 293(3-4):180-186
104.Joerink M, Ribeiro CM, Stet, R J, Hermsen T, Savelkoul HF, Wiegertjes GF. Head kidney-derived macrophages of common carp (Cyprinus carpio L.) show plasticity and functional polarization upon differential stimulation. J Immunol 2006,177(1):61-69
105.Kang M, Oh M, Kim Y, Kawai K, Jung S. Establishment and characterization of two new cell lines derived from flounder, Paralichthys olivaceus (Temminck & Schlegel). J Fish Dis 2003,26(11-12):657-665
106.Katakura Y, Alam S, Shirahata S. Immortalization by gene transfection. Methods Cell Biol 1998,57:69-91
107.Kelly R, Miller H, Nielsen O, Clayton J. Fish cell culture:characteristics of a continuous fibroblastic cell line from Walleye (Stizostedion vitreum vitreum). Can J Fish Aquat Sci 1980,37,1070-1075
108.Kerstner-Wood C, Coward L, Gorman G. U.S. EPA docket AR-226-1354. Washington, DC:U.S. Environmental Protection Agency. Protein Binding of Perfluorobutane Sulfonate, Perfluorohexane Sulfonate, Perfluorooctane Sulfonate and Perfluorooctanoate to Plasma (Human, Rat and Monkey), and Various Human-Derived Plasma Protein Fractions.2003
109.Key BD, Howell RD, Criddle CS. Fluorinated organics in the biosphere. Environ Sci Technol 1997,31(9):2445-2454
110.Kiyono T, Foster SA, Koop JI, McDougall JK, Galloway DA, Klingelhutz AJ. Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 1998,396(6706):84-88
11 l.Komura JI, Mitani H, Shima A. Fish cell culture:Establishment of two fibroblast-like cell lines (OL-17 and OL-32) from fins of the medaka, Oryzias latipes. In Vitro Cell Dev-An 1988,24(4):294-298
112.Kondo H, Watabe S. Temperature-dependent enhancement of cell proliferation and mRNA expression for type I collagen and HSP70 in primary cultured goldfish cells. Comp Biochem Physiol A Mol Integr Physiol 2004,138(2):221-228
113.Ku CC, Teng YC, Wang CS, Lu CH. Establishment and characterization of three cell lines derived from the rockfish grouper Epinephelus quoyanus:Use for transgenic studies and cytotoxicity testing. Aquaculture 2009,294(1-2):147-151
114.Lai YS, John J, Lin CH, Guo IC, Chen SC, Fang K, Chang CY. Establishment of cell lines from a tropical grouper, Epinephelus awoara (Temminck & Schlegel), and their susceptibility to grouper irido-and nodaviruses. J Fish Dis 2003,26(1): 31-42
115.Lai YS, Murali S, Chiu HC, Ju HY, Lin Y S, Chen SC, Guo IC, Fang K, Chang CY. Propagation of yellow grouper nervous necrosis virus (YGNNV) in a new nodavirus-susceptible cell line from yellow grouper, Epinephelus awoara (Temminck & Schlegel), brain tissue. JFish Dis 2001,24(5):299-309
116.Lai YS, Murali S, Ju HY, Wu MF, Guo IC, Chen SC, Fang K, Chang CY. Two iridovirus-susceptible cell lines established from kidney and liver of grouper, Epinephelus awoara (Temminck & Schlegel), and partial characterization of grouper iridovirus. J Fish Dis 2000,23(6):379-388
117.Lakra WS, Swaminathan TR, Rathore G, Goswami M, Yadav K, Kapoor S. Development and characterization of three new diploid cell lines from Labeo rohita (Ham.). Biotechnol Prog 2010,26(4):1008-1013
118.Lakra W, Swaminathan TR, Joy K. Development, characterization, conservation and storage of fish cell lines:a review. Fish Physiol Biochem 2011,37(1):1-20
119.Lakra WS, Sivakumar N, Goswami M, Bhonde RR. Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch). Aquac Res 2006, 37(1):18-24
120.Lamche G, Meier W, Suter M, Burkhardt-Holm P. Primary culture of dispersed skin epidermal cells of rainbow trout Oncorhynchus mykiss Walbaum. Cell Mol Life Sci 1998,54(9):1042-1051
121.Lau C, Anitole K, Hodes C, Lai D, Pfahles-Hutchens A, Seed J. Perfluoroalkyl acids:a review of monitoring and toxicological findings. Toxicol Sci 2007,99(2): 366-394
122.Leguen I, Peron S, Prunet P. Effects of iron on rainbow trout gill cells in primary culture. Cell Biol Toxicol 2011,27(5):311-319
123.Lein NPH, Fujii S, Tanaka S, Nozoe M, Tanaka H. Contamination of perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) in surface water of the Yodo River basin (Japan). Desalination 2008,226(1-3):338-347
124.Levan A, Fredga K, Sandberg AA. Nomenclature for centromeric position on chromosomes. Hereditas 1964,52(2):201-220
125.Lewis D, Marks J. Microcultures of Sarotherodon mossambicus (Peters) cells: Their use in detecting fish viruses. J Fish Dis 1985,8(5):477-478
126.Li SF, Cai WQ. Genetic improvement of the herbivorous blunt snout bream (Megalobrama amblycephala). Naga 2003,26 (1):20-23
127.Lu RH, Liang XF, Wang M, Zhou Y, Bai XL, He Y The role of leptin in lipid metabolism in fatty degenerated hepatocytes of the grass carp Ctenopharyngodon idellus. Fish Physiol Biochem 2012,38(6):1759-1774
128.Lu Y, Aguirre AA, Hamm C, Wang Y, Yu Q, Loh PC, Yanagihara R. Establishment, cryopreservation, and growth of 11 cell lines prepared from a juvenile Hawaiian monk seal, Monachus schauinslandi. Methods Cell Sci 2000, 22(2-3):115-124
129.Luebker DJ, Hansen KJ, Bass NM, Butenhoff JL, Seacat AM. Interactions of flurochemicals with rat liver fatty acid-binding protein. Toxicology 2002,176(3): 175-185
130.Luo W, Zeng C, Deng W, Robinson N, Wang WM, Gao ZX. Genetic parameter estimates for growth-related traits of blunt snout bream(Megalobrama amblycephala) using microsatellite-based pedigree. Aquac Res 2013,1-8, DOI 10.1111/are.12133
131.Matsumoto J, Ishikawa T, Masahito P, Takayama S. Permanent cell lines from erythrophoromas in goldfish(Carassius auratus). JNatl Cancer Inst 1980,64(4): 879-890
132.Matsuo Y, Nishizaki C, Drexler H. Efficient DNA fingerprinting method for the identification of cross-culture contamination of cell lines. Hum Cell 1999,12(3): 149-154
133.Mauger PE, Labbe C, Bobe J, Caury C, Leguen I, Baffet G, Le Bail PY. Characterization of goldfish fin cells in culture:some evidence of an epithelial cell profile. Comp Biochem Physiol B Biochem Mol Biol 2009,152(3):205-215
134.Miller NW, Sizemore R, Clem L. Phylogeny of lymphocyte heterogeneity:the cellular requirements for in vitro antibody responses of channel catfish leukocytes. J Immunol 1985,134(5):2884-2888
135.Mitani H, Naruse K, Shima A. Eurythermic and stenothermic growth of cultured fish cells and their thermosensitivity. J Cell Sci 1989,93(Pt4):731-737
136.Moody CA, Martin JW, Kwan WC, Muir DC, Mabury SA. Monitoring perfluorinated surfactants in biota and surface water samples following an accidental release of fire-fighting foam into Etobicoke Creek. Environ Sci Technol 2002,36(4):545-551
137.Morikawa A, Kamei N, Harada K, Inoue K, Yoshinaga T, Saito N, Koizumi A. The bioconcentration factor of perfluorooctane sulfonate is significantly larger than that of perfluorooctanoate in wild turtles (Trachemys scripta elegans and Chinemys reevesii):an Ai river ecological study in Japan. Ecotoxicol Environ Saf 2006,65(1):14-21
138.Moritz C, Labbe C. Cryopreservation of goldfish fins and optimization for field scale cryobanking. Cryobiology 2008,56(3):181-188
139.Mothersill C, Lyng F, Lyons M, Cottell D. Growth and differentiation of epidermal cells from the rainbow trout established as explants and maintained in various media. JFish Biol 1995,46(6):1011-1025
140.Mulkiewicz E, Jastorff B, Skladanowski A, Kleszczynski K, Stepnowski P. Evaluation of the acute toxicity of perfluorinated carboxylic acids using eukaryotic cell lines, bacteria and enzymatic assays. Environ Toxicol Pharmacol 2007,23(3):279-285
141.Nicholson BL, Byrne C. An established cell line from the Atlantic salmon(Salmo salar). JFish Board Can 1973,30(70):913-916
142.Nicholson B, Danner D, Wu JL. Three new continuous cell lines from marine fishes of Asia. In Vitro Cell Dev-An 1987,23(3):199-204
143.Olsen GW, Gilliland FD, Burlew MM, Burris JM, Mandel JS, Mandel JH. An epidemiologic investigation of reproductive hormones in men with occupational exposure to perfluorooctanoic acid. J Occup Environ Med 1998,40(7):614-622
144.Organisation for Economic Cooperation and Development (OECD). Hazard Assessment of Perfluorooctane Sulfonate (PFOS) and Its Salts. US EPA docket AR-226-1140.2002
145.0u-yang L, Huang XH, Huang EY, Huang YH, Gong J, Sun JJ, Qin QW. Establishment and characterization of a new marine fish cell line derived from red-spotted grouper Epinephelus akaara. J Fish Biol 2010,77(5):1083-1095
146.Parameswaran V, Shukla R, Bhonde R, Hameed AS. Development of a pluripotent ES-like cell line from Asian sea bass(Lates calcarifer)-an oviparous stem cell line mimicking viviparous ES cells. Mar Biotechnol 2007,9(6):766-775
147.Parameswaran V, Shukla R, Bhonde R, Sahul Hameed A. Splenic cell line from sea bass, Lates calcarifer:Establishment and characterization. Aquaculture 2006, 261(1):43-53
148.Pastoor TP, Lee KP, Perri MA, Gillies PJ. Biochemical and morphological studies of ammonium perfluorooctanoate-induced hepatomegaly and peroxisome proliferation. Exp Mol Pathol 1987,47(1):98-109
149.Perry G, McDonald G, Ferguson M, Ganassin R, Bols N. Characterization of rainbow trout cell lines using microsatellite DNA profiling. Cytotechnology 2001, 37(3):143-151
150.Pettersen EF, Ingerslev HC, Stavang V, Egenberg M, Wergeland HI. A highly phagocytic cell line TO from Atlantic salmon is CD83 positive and M-CSFR negative, indicating a dendritic-like cell type. Fish Shellfish Immunol 2008,25(6): 809-819
151.Phelan PE, Pressley ME, Witten PE, Mellon MT, Blake S, Kim CH. Characterization of snakehead rhabdovirus infection in zebrafish (Danio rerio). J Virol 2005,79(3):1842-1852
152.Pombinho AR, Laize V, Molha DM, Marques SM, Cancela ML. Development of two bone-derived cell lines from the marine teleost Sparus aurata; evidence for extracellular matrix mineralization and cell-type-specific expression of matrix Gla protein and osteocalcin. Cell Tissue Res 2004,315(3):393-406
153.Prasanna I, Lakra W, Ogale S, Bhonde R. Cell culture from fin explant of endangered golden mahseer, Tor putitora (Hamilton). Curr Sci India 2000,79(1): 93-94
154.Qin Q, Wu T, Jia T, Hegde A, Zhang R. Development and characterization of a new tropical marine fish cell line from grouper, Epinephelus coioides susceptible to iridovirus and nodavirus. J Virol Methods 2006,131(1):58-64
155.Ribeiro L, Ahne W. Fish cell culture:Initiation of a line of pituitary cells from carp(Cyprinus carpio) to study the release of gonadotropin in vitro. In vitro 1982, 18(5):419-420
156.Rougee L, Ostrander GK, Richmond RH, Lu Y. Establishment, characterization, and viral susceptibility of two cell lines derived from goldfish Carassius auratus muscle and swim bladder. Diseases Aquatic Organ 2007,77(2):127-135
157.Ruiz S, Schyth BD, Encinas P, Tafalla C, Estepa A, Lorenzen N, Coll J. New tools to study RNA interference to fish viruses:Fish cell lines permanently expressing siRNAs targeting the viral polymerase of viral hemorrhagic septicemia virus. Antiviral Res 2009,82(3):148-156
158.Sahul Hameed A, Parameswaran V, Shukla R, Bright Singh I, Thirunavukkarasu A, Bhonde R. Establishment and characterization of India's first marine fish cell line (SISK) from the kidney of sea bass(Lates calcarifer). Aquaculture 2006, 257(1-4):92-103
159.Santos Y, Pazos F, Bandin I, Toranzo AE. Analysis of antigens present in the extracellular products and cell surface of Vibrio anguillarum serotypes 01,02, and 03. Appl Environ Microbiol 1995,61(7):2493-2498
160.Santos-Ruiz L, Santamaria J, Becerra J. Cytoskeletal dynamics of the teleostean fin ray during fin epimorphic regeneration. Differentiation 2005,73(4):175-187
161.Schaffeld M, Markl J. Fish keratins. Methods Cell Biol 2004,78,627-671
162.Shen L, Stuge TB, Zhou H, Khayat M, Barker KS, Quiniou S, Wilson M, Bengten E, Chinchar VG, Clem LW. Channel catfish cytotoxic cells:a mini-review. Dev Comp Immunol 2002,26(2):141-149
163.Shima A, Nikaido O, Shinohara S, Egami N. Continued in vitro growth of fibroblast-like cells (RBCF-1) derived from the caudal fin of the fish, Carassius auratus. Exp Gerontol 1980,15(4):305-314
164.Skutlarek D, Exner M, Farber H. Perfluorinated surfactants in surface and drinking waters. Environ Sci Pollut Res Int 2006,13(5):299-307
165.Sobhana K, George K, Venkat Ravi G, Ittoop G, Paulraj R. Development of a cell culture system from gill explants of the grouper, Epinephelus malabaricus (Bloch and Shneider). Asian Fish Sci 2009,22(2):541-547
166.Sohlenius AK, Andersson K, DePierre JW. The effects of perfluoro-octanoic acid on hepatic peroxisome proliferation and related parameters show no sex-related differences in mice. Biochem J 1992,285(Pt 3):779-783
167.Sohlenius AK, Eriksson AM, Hogstrom C, Kimland M, DePierre JW. Perfluorooctane sulfonic acid is a potent inducer of peroxisomal fatty acid β-oxidation and other activities known to be affected by peroxisome proliferators in mouse liver. Pharmacol Toxicol 1993,72(2):90-93
168.Somamoto T, Nakanishi T, Okamoto N. Specific cell-mediated cytotoxicity against a virus-infected syngeneic cell line in isogeneic ginbuna crucian carp. Dev Comp Immunol 2000,24(6-7):633-640
169. Song Y, Liu L, Shen H, You J, Luo Y. Effect of sodium alginate-based edible coating containing different anti-oxidants on quality and shelf life of refrigerated bream (Megalobrama amblycephala). Food Control 2011,22(3-4):608-615
170.Starkov AA, Wallace KB. Structural determinants of fluorochemical-induced mitochondrial dysfunction. Toxicol Sci 2002.66(2):244-252.
171.Stone D, Ahne W, Denham K, Dixon P, Liu C, Sheppard A, Taylor G, Way K. Nucleotide sequence analysis of the glycoprotein gene of putative spring viraemia of carp virus and pike fry rhabdovirus isolates reveals four genogroups. Dis Aquat Organ 2003,53(3):203-210
172.Swaminathan TR, Lakra WS, Gopalakrishnan A, Basheer V, Khushwaha B, Sajeela K. Development and characterization of a new epithelial cell line PSF from caudal fin of Green chromide, Etroplus suratensis (Bloch,1790). In Vitro Cell Dev Biol Anim 2010,46(8):647-656
173.Tan F, Wang M, Wang W, Lu Y. Comparative evaluation of the cytotoxicity sensitivity of six fish cell lines to four heavy metals in vitro. Toxicol In Vitro 2008, 22(1):164-170
174.Tong SL, Li H, Miao HZ. The establishment and partial characterization of a continuous fish cell line FG-9307 from the gill of flounder Paralichthys olivaceus. Aquaculture 1997,156(3-4):327-333
175.USEPA. Preliminary Risk Assessment of the Developmental Toxicity Associated with Exposure to Perfluorooctanoic Acid and Its Salts.2003
176.Vaziri H, Benchimol S. Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span. Curr Biol 1998,8(5):279-282
177.Wagg SK, Lee LE. A proteomics approach to identifying fish cell lines. Proteomics 2005,5(16):4236-4244
178.Wang G, Lapatra S, Zeng L, Zhao Z, Lu Y. Establishment, growth, cryopreservation and species of origin identification of three cell lines from white sturgeon, Acipenser transmontanus. Meth Cell Sci 2004,25(3-4):211-220
179. Wang N, Wang X, Sha Z, Tian Y, Chen S. Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus). Fish Physiol Biochem 2010a,36(4):1227-1234
180.Wang X, Wang N, Sha Z, Chen S. Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis). Fish Physiol Biochem 2010b,36(4):1181-1189
181.Wei YB, Fan TJ, Jiang GJ, Sun A, Xu XH, Wang J. Establishment of a novel fin cell line from Brown-marbled grouper, Epinephelus fuscoguttatus (Forsskal), and evaluation of its viral susceptibility. Aquac Res 2009,40(13):1523-1531
182. Wei Y, Liu Y, Wang J, Tao Y, Dai J. Toxicogenomic analysis of the hepatic effects of perfluorooctanoic acid on rare minnows (Gobiocypris rarus). Toxicol Appl Pharmacol 2008,226(3):285-297
183.White SS, Calafat AM, Kuklenyik Z, Villanueva L, Zehr RD, Helfant L, Strynar MJ, Lindstrom AB, Thibodeaux JR, Wood C. Gestational PFOA exposure of mice is associated with altered mammary gland development in dams and female offspring. Toxicol Sci 2007,96(1):133-144
184.Wolf K, Mann JA. Poikilotherm vertebrate cell lines and viruses:a current listing for fishes. In Vitro 1980,16(2):168-179
185.Wolf K, Quimby M. Established eurythermic line of fish cells in vitro. Science 1962,135(3508):1065-1066
186.Wondergem R, Amsler K. Transmembrane protentials of cultured rectal gland cells of Raja erinacea. Bull Mt Desert Is1 Bio Lab 1986,26:105-107
187.Wu Y, Chi S. Persistence of betanodavirus in barramundi brain (BB) cell line involves the induction of interferon response. Fish Shellfish Immunol 2006,21(5): 540-547
188.Xiao Q, Li D, Liu H. A flounder (Paralichthys olivaceus) gill cell line as in vitro acute assay system of nonylphenol cytotoxicity. Environ Monit Assess 2011, 175(1-4):315-319.
189.Xing JQ El-Sweisi W, Lee L, Collodi P, Seymour C, Mothersill C, Bols NC. Development of a zebrafish spleen cell line, ZSSJ, and its growth arrest by gamma irradiation and capacity to act as feeder cells. In Vitro Cell Dev Biol Anim 2009,45(3-4):163-174
190.Yan W, Nie P, Lu Y. Establishment, characterization and viral susceptibility of a new cell line derived from goldfish, Carassius auratus (L.), tail fin. J Fish Dis 2011,34(10):757-768
191.Ye HQ, Chen SL, Sha ZX, Xu MY. Development and characterization of cell lines from heart, liver, spleen and head kidney of sea perch Lateolabrax japonicus. J Fish Biol 2006,69(sa):115-126
192.Yi M, Hong N, Hong Y. Generation of medaka fish haploid embryonic stem cells. Science 2009,326(5951):430-433
193.Zhang B, Wang X, Sha Z, Yang C, Liu S, Wang N, Chen SL. Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis. IntJBiol Sci 2011,7(4):452-459
194.Zhang L, Luo Q, Fang Q, Wang Y. An improved RT-PCR assay for rapid and sensitive detection of grass carp reovirus. J Virol Methods 2010,169(1):28-33
195.Zhang X, Xie P, Zhang X, Zhou W, Zhao S, Zhao Y, Cai Y. Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions. J Appl Toxicol 2012, DOI 10.1002/jat.2749
196.Zhao Y, Gul Y, Li S, Wang W. Cloning, identification and accurate normalization expression analysis of PPARa gene by GeNorm in Megalobrama amblycephala. Fish Shellfish Immunol 2011,31(3):462-468
197.Zhao Z, Lu Y. Establishment and characterization of two cell lines from bluefin trevally Caranx melampygus. DisAquat Organ 2006,68(2):91-100
198.Zhao Z, Montgomery-Brock D, Lee CS, Lu Y. Establishment, characterization and viral susceptibility of 3 new cell lines from snakehead, Channa striatus (Blooch). Methods Cell Sci 2004,25(3-4):155-166
199.Zhou GZ, Li ZQ, Yuan XP, Zhang QY. Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara). Mar Biotechnol 2007,9(3):370-376
200.Zhou G, Gui L, Li Z, Yuan X, Zhang Q. Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus. J Fish Biol 2008,73(8):2058-2067
201.Zhou L, Deane E, Woo N. Development of a black sea bream fibroblast cell line and its potential use as an in vitro model for stress protein studies. Fish Physiol Biochem 2003,29(4):255-262
202.Zhu DM, Yang K, Wang WM, Song W. Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala. Fish Physiol Biochem 2013,1-12. DOI 10.1007/s10695-013-9794-6
203.Zou S, Li S, Cai W, Zhao J, Yang H. Establishment of fertile tetraploid population of blunt snout bream (Megalobrama amblycephala). Aquaculture 2004,238(1-4): 155-164.