一个水稻生殖发育突变体的遗传分析及基因定位
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摘要
水稻生殖发育突变体的获得和研究,在揭示生殖发育过程中基因的作用及其相互关系,探明水稻的生殖发育,特别是花发育的奥秘起了十分重要的作用。研究水稻生殖发育机制具有重要的理论意义和实际应用价值。本课题组从明恢86的组培材料T2群体中发现了可以稳定遗传的水稻生殖发育突变体frol(functionof reproductive organ 1),其基因拟名为frol(t)。本研究主要从遗传学以及分子标记基因定位两个方面对frol进行研究,为今后基因表达和克隆等奠定基础。主要的研究结果如下:
frol突变体形态表现为内外稃闭合,抽穗但不开花;4轮器官结构及数目近正常,但内外稃片抱合扭曲成辣椒状,雌雄蕊发育不完全,呈透明色,雌雄蕊均不育。
以frol突变体为父本,明恢86,R527、93-11和中花16为母本配制杂交组合进行性状遗传分析。所有组合F_1代植株均表现为正常亲本的表型,根据F2代表型及X“测验结果表明,正常株与突变株的比例符合1对基因控制的分离比3:1,即该突变性状是受一对隐性基因控制的。
以frol和中花16杂交构建F2为定位群体,采用微卫星标记技术,应用BSA分析方法,并最终将frol(t)定位在第8号染色体inDe10802和RM23432之间。其中RM23432与frol(t)的遗传距离为1.3cM,inDe10802与frol(t)的遗传距离为0.58cM。
The acquisition and research on the rice mutants related to reproductive development played an important role in uncovering the function and interaction of genes in reproductive process,especially in floral development.Research on rice reproductive mechanism is significant both in theory and in human agriculture.The mutant strains called in this paper frol(function of reproductive organ 1)were found by our research group,and the genes involved were named as frol(t)respectively. They were found in T2 population of minghui86 transgenic rice progeny.We have conducted researches on frol in three aspects:morphological observation,genetic analysis and gene mapping,setting strong foundation for gene expressing and gene clonin Genetic analysis of non-pales
Mutant frol had heading but not flowering.Floret shape of frol mutant is similar to that of tiny green capsicum,and in which stamens and pistils are abnormal, translucent and sterile.
Four F2 populations,Minghui86/ mutant,R527/ mutant,93-11/ mutant and Zhonghua16/ mutant were developed,the result of genetic analysisindicated that the mutant is controlled by a single recessive gene in the near future.
Using mutant heterozygotes of frol,F2 populations were developed from the crosses of frol and zhonghua16 to detect molecular markers linked with the gene of frol(t),with the aid of SSR mark technique and by means of BSA analysis.
Finally,the frol(t)was mapped between marker RM23432 and maker inDe10802 in the region of chromsome8.RM23432 was on one side of frol(t)with genetic distance of 1.4 cM,and inDe10802 was on the other side with 0.6 cM respectively.
frol突变体形态表现为内外稃闭合,抽穗但不开花;4轮器官结构及数目近正常,但内外稃片抱合扭曲成辣椒状,雌雄蕊发育不完全,呈透明色,雌雄蕊均不育。
以frol突变体为父本,明恢86,R527、93-11和中花16为母本配制杂交组合进行性状遗传分析。所有组合F_1代植株均表现为正常亲本的表型,根据F2代表型及X“测验结果表明,正常株与突变株的比例符合1对基因控制的分离比3:1,即该突变性状是受一对隐性基因控制的。
以frol和中花16杂交构建F2为定位群体,采用微卫星标记技术,应用BSA分析方法,并最终将frol(t)定位在第8号染色体inDe10802和RM23432之间。其中RM23432与frol(t)的遗传距离为1.3cM,inDe10802与frol(t)的遗传距离为0.58cM。
The acquisition and research on the rice mutants related to reproductive development played an important role in uncovering the function and interaction of genes in reproductive process,especially in floral development.Research on rice reproductive mechanism is significant both in theory and in human agriculture.The mutant strains called in this paper frol(function of reproductive organ 1)were found by our research group,and the genes involved were named as frol(t)respectively. They were found in T2 population of minghui86 transgenic rice progeny.We have conducted researches on frol in three aspects:morphological observation,genetic analysis and gene mapping,setting strong foundation for gene expressing and gene clonin Genetic analysis of non-pales
Mutant frol had heading but not flowering.Floret shape of frol mutant is similar to that of tiny green capsicum,and in which stamens and pistils are abnormal, translucent and sterile.
Four F2 populations,Minghui86/ mutant,R527/ mutant,93-11/ mutant and Zhonghua16/ mutant were developed,the result of genetic analysisindicated that the mutant is controlled by a single recessive gene in the near future.
Using mutant heterozygotes of frol,F2 populations were developed from the crosses of frol and zhonghua16 to detect molecular markers linked with the gene of frol(t),with the aid of SSR mark technique and by means of BSA analysis.
Finally,the frol(t)was mapped between marker RM23432 and maker inDe10802 in the region of chromsome8.RM23432 was on one side of frol(t)with genetic distance of 1.4 cM,and inDe10802 was on the other side with 0.6 cM respectively.
引文
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