囊素对禽流感H5N1亚型灭活疫苗等的免疫增强效果
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摘要
本文研究疫苗常规免疫剂量及减半剂量配合应用囊素对免疫应答的影响。
1．囊素对禽流感H5N1亚型灭活疫苗的免疫增强效果
将7日龄黄羽肉鸡250只分为5组，即对照组、常规疫苗剂量配合囊素组、常规疫苗剂量减半配合囊素组、生理盐水组和囊素组，每组50只。通过血凝抑制试验测定血清抗体水平及计算法氏囊指数等试验方法，研究囊素和禽流感H5N1灭活疫苗配合应用的免疫效果及囊素对母源抗体的影响。结果显示，29日龄后，常规免疫剂量配合囊素组的法氏囊指数和血清抗体滴度显著高于对照组(p＜0.05)，而常规剂量减半配合囊素组与对照组在法氏囊指数和血清抗体滴度上差异不显著(p＞0.05)；囊素组的母源抗体滴度比生理盐水组高1.5-2个滴度。说明囊素对禽流感疫苗有免疫增强作用，疫苗剂量减半配合囊素应用可达到常规疫苗免疫效果，囊素对母源抗体有维持作用。
2．囊素新城疫LaSota株弱毒疫苗的免疫增强效果
将7日龄黄羽肉鸡300只分为5组，即对照组、常规疫苗剂量配合囊素组、常规疫苗剂量减半配合囊素组、生理盐水组和囊素组，每组60只。通过血凝抑制试验测定血清抗体水平和间接ELISA检测鼻腔冲洗液中IgA水平及计算法氏囊指数等试验方法，研究囊素和新城疫LaSota弱毒疫苗配合应用的免疫效果。结果显示，29日龄后，常规免疫剂量配合囊素组的法氏囊指数、血清抗体滴度和IgA水平显著高于对照组(p＜0.05)，而常规剂量减半配合囊素组与对照组在法氏囊指数、血清抗体滴度和IgA水平上差异不显著(p＞0.05)；说明囊素对疫苗有免疫增强作用，疫苗剂量减半配合囊素应用可达到常规疫苗免疫效果。
3．囊素对传染性法氏囊病弱毒疫苗的免疫增强效果
将7日龄黄羽肉鸡300只分为5组，即对照组、常规疫苗剂量配合囊素组、常规疫苗剂量减半配合囊素组、生理盐水组和囊素组，每组60只。通过间接ELISA测定血清抗体水平及计算法氏囊指数等试验方法，研究囊素和传染性法氏囊病毒弱毒疫苗配合应用的免疫效果。结果显示，常规免疫剂量配合囊素组和疫苗剂量减半配合囊素组的法氏囊指数显著高于对照组，常规免疫剂量配合
Here, this paper mainly studied the effect of the normal immune dose and the half of that for vaccine associated with bursin on immune response. 1.The immune enhancement of bursin on avian influenza virus (AIV) H5N1 subtype inactivated vaccine
To investigate the immune enhancement effect of bursin on inactivated avian influenza virus (AIV) H5N1 subtype vaccine and maternal antibody, two hundred and fifty huang broiler chickens were divided into 5 groups of 50 each as follows: the group for control, normal vaccine immunized dose associated with bursin, half of normal vaccine immunized dose included with bursin, saline and bursin. Hemagglutination inhibition assays and calculation of index of bursa of fabricius and spleen were applied during experiment. After 29 days old, significant difference at index of bursa of fabricius and HI antibody titer of serum can be observed between the group of normal immune dose accompanied with bursin and that of control (p＜0.05) , however, no difference can be discerned between the group of half of normal vaccine immune dose including bursin and the control one (p>0.05). Antibody titer of the bursin group was higher than that of the saline one between 1.5 and 2 titer. The present results show that there exist the immune enhancement effects of bursin on the chicken's immune response. In addition, the same immune effect as the normal immunized group can be induced by half of normal immune dose associated with
peptide. The role bursin played in maintaining maternal antibody.
2.The immune enhancement of bursin on LaSota strain of Newcastle disease
virus (NDV) live -attenuated vaccine
To investigate the immune enhancement effect of bursin on live-attenuated
1．囊素对禽流感H5N1亚型灭活疫苗的免疫增强效果
将7日龄黄羽肉鸡250只分为5组，即对照组、常规疫苗剂量配合囊素组、常规疫苗剂量减半配合囊素组、生理盐水组和囊素组，每组50只。通过血凝抑制试验测定血清抗体水平及计算法氏囊指数等试验方法，研究囊素和禽流感H5N1灭活疫苗配合应用的免疫效果及囊素对母源抗体的影响。结果显示，29日龄后，常规免疫剂量配合囊素组的法氏囊指数和血清抗体滴度显著高于对照组(p＜0.05)，而常规剂量减半配合囊素组与对照组在法氏囊指数和血清抗体滴度上差异不显著(p＞0.05)；囊素组的母源抗体滴度比生理盐水组高1.5-2个滴度。说明囊素对禽流感疫苗有免疫增强作用，疫苗剂量减半配合囊素应用可达到常规疫苗免疫效果，囊素对母源抗体有维持作用。
2．囊素新城疫LaSota株弱毒疫苗的免疫增强效果
将7日龄黄羽肉鸡300只分为5组，即对照组、常规疫苗剂量配合囊素组、常规疫苗剂量减半配合囊素组、生理盐水组和囊素组，每组60只。通过血凝抑制试验测定血清抗体水平和间接ELISA检测鼻腔冲洗液中IgA水平及计算法氏囊指数等试验方法，研究囊素和新城疫LaSota弱毒疫苗配合应用的免疫效果。结果显示，29日龄后，常规免疫剂量配合囊素组的法氏囊指数、血清抗体滴度和IgA水平显著高于对照组(p＜0.05)，而常规剂量减半配合囊素组与对照组在法氏囊指数、血清抗体滴度和IgA水平上差异不显著(p＞0.05)；说明囊素对疫苗有免疫增强作用，疫苗剂量减半配合囊素应用可达到常规疫苗免疫效果。
3．囊素对传染性法氏囊病弱毒疫苗的免疫增强效果
将7日龄黄羽肉鸡300只分为5组，即对照组、常规疫苗剂量配合囊素组、常规疫苗剂量减半配合囊素组、生理盐水组和囊素组，每组60只。通过间接ELISA测定血清抗体水平及计算法氏囊指数等试验方法，研究囊素和传染性法氏囊病毒弱毒疫苗配合应用的免疫效果。结果显示，常规免疫剂量配合囊素组和疫苗剂量减半配合囊素组的法氏囊指数显著高于对照组，常规免疫剂量配合
Here, this paper mainly studied the effect of the normal immune dose and the half of that for vaccine associated with bursin on immune response. 1.The immune enhancement of bursin on avian influenza virus (AIV) H5N1 subtype inactivated vaccine
To investigate the immune enhancement effect of bursin on inactivated avian influenza virus (AIV) H5N1 subtype vaccine and maternal antibody, two hundred and fifty huang broiler chickens were divided into 5 groups of 50 each as follows: the group for control, normal vaccine immunized dose associated with bursin, half of normal vaccine immunized dose included with bursin, saline and bursin. Hemagglutination inhibition assays and calculation of index of bursa of fabricius and spleen were applied during experiment. After 29 days old, significant difference at index of bursa of fabricius and HI antibody titer of serum can be observed between the group of normal immune dose accompanied with bursin and that of control (p＜0.05) , however, no difference can be discerned between the group of half of normal vaccine immune dose including bursin and the control one (p>0.05). Antibody titer of the bursin group was higher than that of the saline one between 1.5 and 2 titer. The present results show that there exist the immune enhancement effects of bursin on the chicken's immune response. In addition, the same immune effect as the normal immunized group can be induced by half of normal immune dose associated with
peptide. The role bursin played in maintaining maternal antibody.
2.The immune enhancement of bursin on LaSota strain of Newcastle disease
virus (NDV) live -attenuated vaccine
To investigate the immune enhancement effect of bursin on live-attenuated
引文
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[1] Jean M, Hammel MD, William K, et al. Update on emerging infections: News from the centers for disease control and prevention[J]. Annals of Emergency Medicine, 2005(45):88-90.
[2] Ilaria Capua, Dennis J Alexander. An update on avian influenza in poultry[J]. International Congress Series, 2004(1263):741-744.
[3] Suarez DL, Schultz-Cherry S. Immunology of avian influenza virus: a review [J]. Developmental and Comparative Immunology 2000(24):269-283.
[4] Brand A, Cilmour DG, Coldstein G. Lymphocyte - differentiating hormone of bursa of Fabricius[J]. Science, 1976(193):319-321.
[5] Audhya T, Kroon D,Heavner G et al. Tripetide structure of bursin, a selective B cell differentiating hormone of the bursa of Fabricius[J]. Science, 1986(231):997-999.
[6] 陆承平．兽医微生物学(第三版)[M]．北京：中国农业出版社，2003．
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