高压脉冲电场常温快速提取中国林蛙多糖工艺机理研究
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摘要
我国在中药有效成分提取方法上以传统方法为主,提取时间长、提取过程繁琐、提取出来的有效成分复杂、含量比例较低,急需开发新的方法提取传统的中药资源,增强我国传统中药的优势,加快中医药走向世界的步伐。
高压脉冲电场(PEF)杀菌技术已被公认为国际上研究最热门、最先进的灭菌技术之一,是对传统灭菌方法的革命,其创新性、科学性和先进性不容置疑。本文根据PEF技术对微生物细胞的作用机理,首次把PEF技术应用到经济药用动物中国林蛙有效成分多糖的提取、分离上,为中药有效成分的提取提供了一条全新的途径,以便于深入发展和拓宽PEF技术的应用范围,同时满足中药现代化对低能耗、低操作费用、高效的高新技术的要求。
经过大量的实验研究,获得了合理的加工工艺,并通过对PEF提取法和常规方法的比较、提取机理的分析、提取动力学模型的建立,证明这种方法是完全可行的,并对该方法的可行性做出了理论解释,建立了一套可以快速提取的工艺方法,为该方法的工业化生产奠定了理论和实践的基础。
Pulsed electric field (PEF) non-thermal pasteurization technique was known as the one of the most interesting and advanced technology on international. It is a revolution in traditional pasteurization technique. It is unassailable that it is innovative, scientific and advanced. It is noticeable that PEF technology is a crossed branch of electronics, chemistry, microbiology, physics and engineering technique. Its mechanism research to microorganism inactivation will provide a new method for life sciences question desiderated to resolve, such as cell components extraction, cell amalgamation et al. But there isn’t any research in this way. So it is necessary to develop the PEF technology to meet the market’s need have low consumed and high effect to a new technique.
The extraction, separation and purification are the key basic technologies of Chinese drug modernization. Because the essential components or the cluster of essential components must be attained, the modernization of Chinese drug may be carried out. Nowadays, the traditional extraction methods give priority to the Chinese drug modernization, which are low effect, long time low extraction quantity. So it is in dire need of a new method to quicken the Chinese drug’s step to the world.
We come to a conclusion on the PEF as follows: extracting at normal temperature, the essential components without loss and denaturalization, time of extraction being short, the achievable yield being high, purification and separation being easy, economizing on energy and without contamination. PEF is better than the traditional technologies The PEF technology is firstly used to extract Rana temporaria chensinensis David’s polysaccharide in this paper. Several key technical problems of the technology are studied as follows:
1. PEF technology is used to extract Rana temporaria chensinensis David’s polysaccharide first time. This is a new exploration and probing to extract the essential components from Chinese herbs. The affect factors of PEF are analyzed with the extraction of Rana temporaria chensinensis David’s polysaccharide in detail. The basic parameters’ranges of PEF to extract the Rana temporaria chensinensis David’s polysaccharide are as following: electric field intensity 20kV/cm, pulse number 6, extraction liquid 0.5%KOH. These results not only provide experimental and theoretical basis more for extracting Rana temporaria
高压脉冲电场(PEF)杀菌技术已被公认为国际上研究最热门、最先进的灭菌技术之一,是对传统灭菌方法的革命,其创新性、科学性和先进性不容置疑。本文根据PEF技术对微生物细胞的作用机理,首次把PEF技术应用到经济药用动物中国林蛙有效成分多糖的提取、分离上,为中药有效成分的提取提供了一条全新的途径,以便于深入发展和拓宽PEF技术的应用范围,同时满足中药现代化对低能耗、低操作费用、高效的高新技术的要求。
经过大量的实验研究,获得了合理的加工工艺,并通过对PEF提取法和常规方法的比较、提取机理的分析、提取动力学模型的建立,证明这种方法是完全可行的,并对该方法的可行性做出了理论解释,建立了一套可以快速提取的工艺方法,为该方法的工业化生产奠定了理论和实践的基础。
Pulsed electric field (PEF) non-thermal pasteurization technique was known as the one of the most interesting and advanced technology on international. It is a revolution in traditional pasteurization technique. It is unassailable that it is innovative, scientific and advanced. It is noticeable that PEF technology is a crossed branch of electronics, chemistry, microbiology, physics and engineering technique. Its mechanism research to microorganism inactivation will provide a new method for life sciences question desiderated to resolve, such as cell components extraction, cell amalgamation et al. But there isn’t any research in this way. So it is necessary to develop the PEF technology to meet the market’s need have low consumed and high effect to a new technique.
The extraction, separation and purification are the key basic technologies of Chinese drug modernization. Because the essential components or the cluster of essential components must be attained, the modernization of Chinese drug may be carried out. Nowadays, the traditional extraction methods give priority to the Chinese drug modernization, which are low effect, long time low extraction quantity. So it is in dire need of a new method to quicken the Chinese drug’s step to the world.
We come to a conclusion on the PEF as follows: extracting at normal temperature, the essential components without loss and denaturalization, time of extraction being short, the achievable yield being high, purification and separation being easy, economizing on energy and without contamination. PEF is better than the traditional technologies The PEF technology is firstly used to extract Rana temporaria chensinensis David’s polysaccharide in this paper. Several key technical problems of the technology are studied as follows:
1. PEF technology is used to extract Rana temporaria chensinensis David’s polysaccharide first time. This is a new exploration and probing to extract the essential components from Chinese herbs. The affect factors of PEF are analyzed with the extraction of Rana temporaria chensinensis David’s polysaccharide in detail. The basic parameters’ranges of PEF to extract the Rana temporaria chensinensis David’s polysaccharide are as following: electric field intensity 20kV/cm, pulse number 6, extraction liquid 0.5%KOH. These results not only provide experimental and theoretical basis more for extracting Rana temporaria
引文
1. 中华人民共和国卫生部药典委员会. 中华人民共和国药典[M]. 广州:广东出版社,1995:223
2. 王春青,吕树臣,李荣权. 中国林蛙的开发与利用[J]. 当代畜牧,2002,8:24-25.
3. 崔桂友. 哈士蟆油的形态结构观察和化学成分测定. 生物学杂志,2000,17(1):27~28.
4. 金莉莉,刘德文,范文今,等. 中国林蛙卵、卵油及输卵管对红细胞 SOD 活性的影响. 中国公共卫生,2002,18(5):607.
5. 崔贞玉,王琳,张琨,等. 林蛙油对老年雌性大鼠脂质过氧化的影响. 环境与职业医学,2002,19(3):204.
6. 于勇,刘阳,范文今,等. 林蛙油耐缺氧与调节血脂的作用. 环境与职业医学,2002,19(3):204~205.
7. 李成义,孙文伟,姜熙罗,等. 蛤士蟆卵油化学成分分析. 中国药学杂志,1994,29(12):712~713.
8. 李成义,李静波,李广文,等. 吉林产蛤士蟆卵油中微量元素含量测定. 中药材,1991,14(1):16~17.
9. 卢立明,宋少江,徐绥绪,等. 蛤士蟆卵油化学成分研究. 中国药物化学杂志,2001,11(4):224~225.
10. 彭飞,徐峰,刘彬,等. 中国林蛙卵油对高脂血症大鼠降脂作用的观察. 医学理论与实践,2003,16(9):996~997.
11. 彭飞,于虹,徐峰,等. 中国林蛙卵油的中枢抑制作用. 沈阳药科大学学报,2002,19(3):204~207.
12. 杜景新,王丽,燕杰. 中国林蛙各部位氨基酸含量的分析. 人参研究,2003,3:40~42.
13. 张晓静,刘会东.植物多糖提取分离及药理作用的研究进展.时珍国医国药,2003,14(8):494~497
14. 魏培莲.微生物胞多糖研究进展.浙江科技学院学报,2003,14(2):8~12
15. 骆传环,黄荣清,刘曙晨.海星多糖的分子量测定.军事医学科学院院刊,2002,26(2).
16. 樊绘曾、陈菊娣等.玉足海参酸性多糖的研究.药学学报,1983,18(3):203~208
17. 许东晖,王兵,许实波,等.鲍鱼多糖对荷瘤小鼠腹腔巨噬细胞迟发型超敏反应的作用.中药材,1999,22(2):88
18. 胡健饶,曹明富.三角帆蚌多糖抑瘤作用的实验研究.中国现代应用药学杂志,2003,20(1):11~13
19. 窦昌贵,黄芳,黄罗生,等.文蛤多糖抗癌免疫药理作用的研究.中国海洋药物,1999,18(2):15
20. 唐巍然,闻杰,于晓红,等.鹿茸多糖对小鼠免疫功能的影响.中医药信息,1998,15(6):43
21. 黄林清,周世文,张诗平,等.蚕蛹多糖对小鼠免疫功能的影响.解放军药学学报,2002,18(1):11~13.
22. 邱鹏新,黎明涛,等.黑海参多糖对 β-淀粉样蛋白诱导的皮质神经元凋亡的保护作用.中草药,2000,31(4):271~274
23. 吕昌龙,赫甡,刘北星,等.乌贼墨及其提取物的免疫刺激作用.中国海洋药物,1999,18(2):32
24. 李东霞,李德全,张双全.鲨鱼软骨多糖的理化性质及其与 DNA 分子相互作用的研究.海洋科学,2000,24(5):40~43
25. 赵本树,杜晓东,吕金梁,等.鲨鱼软骨酸性多糖对小鼠的降脂作用.中国药理学通报,1995,11(3):259.
26. 高存记,李家增,彭林,等.刺参酸性粘多糖对纤维蛋白凝胶结构及其溶解性的影响.中华血液学杂志,1996,17(9):458
27. 王大为,张艳荣,沙坤.中国林蛙皮多糖类天然保湿因子的提取与鉴定.吉林农业大学学报,2002,24(6):99~102,106
28. 钱晋,过鑫昌,王学锋,等.玉足海参酸性粘多糖对冠心病患者血凝、血液粘度及血脂的影响.上海医学,1997,20(6):342
29. 钦传光,黄开勋,徐辉碧.泥鳅多糖的免疫作用研究.中国药学杂志,2002,37(8):
588~591.
30. 钦传光,周军,赵文,等.泥鳅多糖清除活性氧自由基和保护 DNA 链的作用.生物化学与生物物理学报,2001,33(2):215~218.
31. 钦传光,黄开勋,徐辉碧.泥鳅及其黏液抗炎作用的实验研究[J].中国药学杂志,2000,35(2):846~847.
32. 钦传光,黄开勋,徐辉碧.泥鳅多糖对试验型糖尿病小鼠血糖血脂的影响[J].中国药理学与毒理学杂志,2002,16(2):124~127.
33. 苏秀榕,娄永江,等.海参的营养成分及海参多糖的抗肿瘤活性的研究.营养学报,2003,25(2):181~183
34. 丁素菊,郑惠民,史荫绵.中性粘多糖对体外超氧阴离子自由基的研究.第二军医大学学报,1994,15(5):464.
35. 李志军,李八方,等.五种海洋多糖抗疲劳作用的初步研究.中国海洋药物,2003,2:53~56
36. 张彦民,李宝才,朱利平,等.多糖化学及其生物活性研究进展.昆明理工大学学报(理工版),2003,28(3):140~149.
37. 刘天龙,许剑琴.多糖现代研究及应用进展.中国兽医杂志,2004,40(3):24~26.
38. 马宝瑕,陈新,邓军娥.中药多糖研究进展.中国医院药学杂志,2003,23(6):360~362
39. 沈鸣,陈建伟.氨基多糖的药理研究进展.上海医药,2001,22(6):268~269.
40. 王琪琳,王海仁.海洋生物多糖药用功能的新进展.生物学通报,2002,37(7):12~13.
41. Murray HG,et al. Scheur PJ (ed):Bioorganic Marine Chemistry I,Springer-Kerlag,Berlin Heidelberg,1987,107~121.
42. Umezawa H, ckami Y, okurasawa S etal. Marinactan antitumor polysaccharide produced by marine bacteria [J]. Antibiot, 1983, 36: 371~376.
43. Nakashima H, kido Y. kokayashi N, et al. Antiretroviral activity in a marine Red alga:reverse extract of schizymenia pacifica [J]. J cancer Red Clin oncol, 1987,113(5):413-419.
44. Cassaro C M F, et. Distribution of sulphated mucopolysaccharides in invertebrates. J Biochem, 1977,252:2254~2259.
45. Ovodov Y S. et al. Polysaccharides of some Japan Sea invertebrates. Comp Biochem physiol, 1969, 29:1093~1099.
46. Rianchini P, et al. Fraction and identification of heparin and other acidic mucopolysaccharides by a new discontinuous electrophoretic method. J Chromalogr, 1980,196:455~461.
47. Dietrich C P, et al. Identification of acidic mucopolysaccharides by agarose gel electrophoresis [J] Chromatogr, 1977,130:299~235.
48. Guerard F, et al., Comparative Studies on Fish Skin Mucins, Program and Abstracts. Second International Marine Biotechnology Conference (IMBC’91),1991,p29.
49. 周铭东,崔悦礼,陈静华等.贝类多糖的组成及性质研究.云南大学学报(自然科学版)1998,20(3):187~189.
50. 姚新生.天然药物化学[M].第三版.北京:人民卫生出版社,2002.53~106.
51. Quass, D. W. Pulsed electric field processing in the food industry. A status report on PEF. Palo Alto, CA. Electric Power Research Institute. CR-109742. 1997.
52. Palaniappan S,Sasfry S K. Effects of electricity on microorganisms:a review [J].J Food Process Preserv,1990,14:393~414.
53. 时兰春,王伯初. 高压电脉冲灭菌理论的研究进展.重庆大学学报,2002,25(4):144~147.
54. Sale A J H, Hamilton W A. Effects of high electric fields on microorganisms [J].Biochim Biophys Acta, 1967,(148):781~788.
55. Hulsheger H, Niemann E G. Lethal effects of high-voltage pulses on E.coli K12 [J].Radiat Environ Biophys,1980,(22):149~162.
56. Zimmermann U . Electric breakdown , electropermeabilization and electrofusion Rev[J].Phys Biochem Phamacol,1986,105:196~256.
57. Vego-Mercado H, Pothakamury U R, Chang F J, et al. Inactivation of E.coli by combining pH, ionic strength and pulsed electric fields hurdles[J]. Food Res Int, 1996,29(2):117~121.
58. Tsong T Y. Electroporation of cell membranes [J]. Biophys J. 1991,60:297~306.
59. Macgrecor S J,Farish 0,Fouracre R,et a1.Inactivation of pathogenic and spoilage microorganisms in a test liquid using pulsed electric fields [J].IEEE transactions on plasma science,2000,28(1):144~149.
60. Pothakamury, U. R., Monsalve-Gonzalez, A., Barbosa-Cánovas, G. V. and Swanson, B. G.Inactivation of Escherichia coli and Staphylococcus aureus in model foods by pulsed electric field technology. Food Res Int. 1995.28(2):167~171.
61. Martin-Belloso, O., Qin, B. L., Chang, F. J., Barbosa-Cánovas, G. V. and Swanson, B. Inactivation of Escherichia coli in skim milk by high intensity pulsed electric fields. J Food Process Eng. 1997 20:317~336.
62. Zhang, Q. H., Qin, B.-L., Barbosa-Cánovas, G. V. and Swanson, B. G. Inactivation of E. coli for food pasteurization by high-strength pulsed electric fields. J Food Process Preserv. 1995, 19(2):103~118.
63. Qin, B., Pothakamury, U. R., Vega, H., Martin, O., Barbosa-Cánovas, G. V. and Swanson, B. G. Food pasteurization using high intensity pulsed electric fields. J Food Technol. 1995,49(12):55~66.
64. Vega-Mercado, H., Martin-Belloso, O., Qin, B.-L., Chang, F.-J., Gongora-Nieto, M. M., Barbosa-Cánovas, G. V. and Swanson, B. G. Non-thermal food preservation: pulsed electric fields. Trends Food Sci Technol. 1997,8(5):151~157.
65. Ma, L., Chang, F. J. and Barbosa-Cánovas, G. V. Inactivation of E. coli in liquid whole eggs using pulsed electric fields technologies. New frontiers in food engineering. Proceedings of the Fifth Conference of Food Engineering. American Institute of Chemical Engineers. 1997.216~221.
66. Qin, B.-L., Barbosa-Cánovas, G. V., Swanson, B. G. and Pedrow, P. D. Inactivating microorganism using a pulsed electric field continuous treatment system. IEEE Trans Indus Applic. 1998,34(1):43~49.
67. Zhang, Q. H., Qiu, X. and Sharma, S. K. Recent development in pulsed electric field processing. Washington, DC. National Food Processors Association. New Technologies Yearbook. 1997,31~42.
68. Dunn, J. E. and Pearlman, J. S. Methods and apparatus for extending the shelf-life of fluid food products. Maxwell Laboratories, Inc. U. S. Patent 1987,4,695,472.
69. Sitzmann, V. High voltage pulse techniques for food preservation. G. W. Gould. New methods for food preservation. London, UK. Blackie Academic and Professional. 1995,236~252.
70. Grahl, T., Sitzmann, W. and Markl, H. Killing of microorganisms in fluid media by high-voltage pulses. DECHEMA Biotechnology Conferences. 1992,675~679.
71. Ho, S. Y., G.S., M., Cross, J. D. and Griffiths, M. W. Inactivation of Pseudomonas fluorescens by high voltage electric pulses. J Food Sci. 1995,60(6):1337~1343.
72. Ho, S. Y., Mittal, G. S. and Cross, J. D. Effects of high field electric pulses on the activity of selected enzymes. J Food Eng. 1997,31(1):69~84.
73. Marquez, V. O., Mittal, G. S. and Griffiths, M. W. Destruction and inhibition of bacterial spores by high voltage pulsed electric field. J Food Sci. 1997,62(2):399~401,409
74. Mittal, G. S. and Choundry, M. Pulsed electric field sterilization of waste brine solution.Proceedings of the Seventh International Congress on Engineering and Food. Brighton Center, UK. 1997,C13~C16.
75. Hulsheger, H., Pottel, J. and Niemann, E. G. Electric field effects on bacteria and yeast cells. Radiat Environ Biophys. 1983,22:149~162
76. Grahl, T. and Maerkl, H. Killing of microorganisms by pulsed electric fields. Applied MicrobiolBiotechnol. 1996,45(1/2):148~157
77. 曾新安,陈勇主编.脉冲电场非热灭菌技术.中国轻工业出版社,2005
78. Qin, B. L., Zhang, Q., Barbosa-Cánovas, G. V., Swanson, B. G. and Pedrow, P. D. Inactivation of microorganisms by pulsed electric fields with different voltage waveforms. IEEE Trans Dielec Insul. 1994,1(6):1047~1057
79. Qin, B.-L., Zhang, Q. H., Barbosa-Cánovas, G. V., Swanson, B. G. and Pedrow, P. D. Pulsed electric field treatment chamber design for liquid food pasteurization using a finite element method. Transactions of the ASAE. 1995,38(2):557~565
80. Mizuno, A. and Hori, Y. Destruction of living cells by pulsed high-voltage application. IEEE Trans Ind Appl. 1991,24(3):387~394
81. Zhang, Q. H., Monsalve-Gonzalez, A., Barbosa-Cánovas, G. V. and Swanson, B. G. Inactivation of E. coli and S. cerevisiae by pulsed electric fields under controlled temperature conditions. Transactions of the ASAE. 1994,37(2):581~587
82. Zhang, Q. H., Chang, F.-J. and Barbosa-Cánovas, G. V. Inactivation of microorganisms in a semisolid model food using high voltage pulsed electric fields. Lebensm Wiss Technol. 1994,27(6):538~543
83. Fernandez-Molina, J. J., Barkstrom, E., Torstensson, P., Barbosa-Cánovas, G. V. and Swanson, B. G. Shelf-life extension of raw skim milk by combining heat and pulsed electric fields. Food Res Int. 1999.
84. Calderon-Miranda, M. L. Inactivation of listeria inocua by pulsed electric fields and nisin. Pullman, WA. Washington State University. 1998.
85. Qin, B.-L., Barbosa-Cánovas, G. V., Swanson, B. G. and Pedrow, P. D. Inactivating microorganism using a pulsed electric field continuous treatment system. IEEE Trans Indus Applic. 1998,34(1):43~49
86. Liu, X., Yousef, A. E. and Chism, G. W. Inactivation of Escherichia coli O157:H7 by the combination of organic acids and pulsed electric field. J Food Safety. 1997,16(4):287~299
87. Simpson, M. V., Barbosa-Cánovas, G. V. and Swanson, B. G. The Combined inhibitory effect of lysozyme and high voltage pulsed electric fields on the growth of Bacillus subtilis spores. IFT Annual Meeting: Book of Abstracts. 1995,267.
88. Pagan, R., Esplugas, S., Gongora-Nieto, M. M., Barbosa-Cánovas, G. V. and Swanson, B. G. Inactivation of Bacillus subtilis spores using high intensity pulsed electric fields in combination with other food conservation technologies. Food Scie Technol Int. 1998,4(1):33~44
89. Castro, A. J. Pulsed electrical field modification of activity and denaturation of alkaline phosphatase. Food Science and Human Nutrition. Pullman, WA. Washington State University. 1994.
90. Vega-Mercado, H., Powers, J. R., Barbosa-Cánovas, G. V. and Swanson, B. G. Plasmin inactivation with pulsed electric fields. J Food Sci. 1995,60(5):1143~1146
91. Yeom,H.W.,Zhang,Q.H.,Dunne,C.P.Inactivation of papain by pulsed electric fields in a continuous system. Food Chemistry.1999,67,53~59.
92. Barsotti, L.,Dumay, E.,Mu, T.H.,Fernandez Diaz,M.D.,Cheftel,J.C.Effects of high voltage electric pulses on proteinbased food constituents and structures. Trends in food Science and Technology. 2001,12(3):136~144
93. 钟葵,孙志健等,高压脉冲电场对辣根过氧化物酶活性及构象的影响效果。中国食品学报,2005,5(1):31~35
94. Joaquin Giner, Vicente Gimeno, Alexandre Eapachs, et al. Inhibition of tomato Licopersicon esculentum Mill. Pectin methylesterase by pulsed electric fields. Innovative Food Science and Emerging Technologies. 2000,1:57~67.
95. S.Min, S.K.Min, et al.Inactivation kinetics of tomato juice lipoxygenase by pulsed electric field. Food Engineering Physical Properties. 2003,68(6):1995~2001.
96. Giner,J.,Rauret-Arino,A.,et al. Inactivation of polyphenoloxidase by pulsed electric fields. In proceedings IFT Annual Meeting, 1997,19,Orlando, USA.
97. Seacheol Min, Z.Tony Jin,and Q.Howard Zhang.Commercial scale PEF processing of tomato juice.J.Agric.Food Chem. 2003,51:3338~3344.
98. Z.Tony Jin and Q.Howard Zhang. PEF inactivation of microorganisms and preservation of quality of cranberry juice. Jounal of Food Processing Preservation.1999,23,481~497.
99. L.Barsotti,P.Merle and J.C.Cheftel. Food processing by PEF I:Physical aspects.Ⅱ .Biological aspects Food Rev. Int.1999,15(2):163~213.
100. Dunn, J. Pulsed light and pulsed electric field for foods and eggs. Poul Sci. 1996,75(9):1133~1136
101. Fernandez-Molina, J. J., Barkstrom, E., Torstensson, P., Barbosa-Cánovas, G. V. and Swanson, B. G. Shelf-life extension of raw skim milk by combining heat and pulsed electric fields. Food Res Int. 1999.
102. Reina, L. D., Jin, Z. T., Yousef, A. E. and Zhang, Q. H. Inactivation of Listeria monocytogenes in milk by pulsed electric field. J Food Protect. 1998,61(9):1203~1206
103. Knorr, D., Geulen, M., Grahl, T. and Sitzmann, W. Food application of high electric field pulses. Trends Food Sci Technol. 1994, 5:71~75
104. 方胜,孙学兵,陆守道.利用高压脉冲电场加速冰解冻的试验研究.北京工商大学学报,2003,21(4):43~53
105. Ngo Vu. Electric field effects on drop size and terminal velocity in liquid-liquid systems. AIChEJ. 1986,32(10):1739~1742
106. Costerton J W, Irvin R T, Cheng K J. The bacterial glycocalyx in nature and disease. Annu Rev Microbiol, 1999,35:299~327
107. 刘学湘,陈建伟. 梅花参中梅花参多糖的提取工艺研究[J]. 南京中医药大学学报,2003,19(3):161~163
108. 李熙灿,曾和平,杨世柱. 黑海参多糖组成的研究[J]. 广州化学,1998(1):17~21.
109. 孙兴权,胡耀辉,付蕾,于晓斌.中国林蛙头中抗凝物质的分离纯化及抗凝活性研究[J].吉林农业大学学报,2000,22(3):100~103.
110. Kennedy JF, Proteoglycans, Elsevier, Amsterdam, 1979
111. Danishefsky I, et al., J Biochem, 1966, 241:143
112. 樊绘曾. 海洋药物,1983,1:40
113. Karamanos NK, et al. Biochem Biophys Acta,1988,966(1):36
114. 王长云,管华诗.氨基多糖的提取、分离和分析测定方法及其研究进展.中国海洋药物,1996,1:24~31
115. 张惟杰.糖复合物生化研究技术[M].杭州:浙江大学出版社,1999,128.
116. Fenichel RL. Immune modulation agents and their mechanism[M]. New York:Marcel Dekker inc, 1984,409.
117. 张豁中,温玉麟. 动物活性成分化学[M].天津:天津科学技术出版社,1999,1~186.
118. 马常夫. 哈士蟆的年龄鉴定与种群结构的初步研究[J]. 东北师范大学学报(自然科学版),1985(1):81~89.
119. 沈鸣,陈建伟. 糙海参多糖纯化工艺优选及其产物的药理活性比较[J]. 中国海洋药物,2003(1):1~4.
120. 任露泉编著.试验优化设计与分析(第 1 版).长春:吉林科学技术出版社,2001,5
121. 涂国云,卜宗式,杜昱光. 酶法提取竹荪深层发酵菌丝体多糖的研究. 中国食用菌,1998,17(1),36~38
122. 林宇野,杨虹. 酶法提取多糖的研究. 食品与发酵工业,1995(1):13~17
123. 陈哲超,林宇野. 碱解、酶解复合法提取香菇多糖的研究. 生物工程进展,1995,15(1):47~50
124. 韩慧敏,谢安,罗杰英. 海参多糖提取工艺研究[J]. 湖南中医杂志,2000,16(1):56~57.
125. 王长云,管华诗,刘树青. 扇贝糖胺聚糖的琼脂糖凝胶电泳分析[J]. 中国海洋药物,1996,2:12~14
126. 孙兴权,付蕾,曹云龙,胡耀辉.林蛙多糖(TCPS)提取工艺的初步研究 [J].吉林农业大学学报,2001,23(4):108~110
127. 冯年平,郁威主编.中药提取分离技术原理与应用.中国医药科技出版社,2005 年
128. 赵兵,苏立民.电场絮凝提取碱性蛋白酶的研究.化工冶金,1991,12(2):143~149
129. 赵兵,苏立民.电场絮凝分离提取 α-淀粉酶.化工学报,1991,(1):109~113
130. Zhang, Q. H., Monsalve-Gonzalez, A., Barbosa-Cánovas, G. V. and Swanson, B. G. Inactivation of E. coli and S. cerevisiae by pulsed electric fields under controlledtemperature conditions. Transactions of the ASAE. 1994,37(2):581~587
131. Dunne, C. P., Dunn, J., Clark, W., Ott, T. and Bushnell, A. H. Application of high energy electric field pulses to preservation of foods for combat rations. Science and Technology for Force XXI. Department of the Army. Norfolk, Virginia. 1996, June 24~27. 7.
132. Jeyamkondan, S., Jayas, D. S. and Holley, R. A. Pulsed electric field processing of foods: a review. J Food Protect. 1999,62(9):1088~1096
133. Schoenbach, K. H., Peterkin, F. E., Alden, R. W. and Beebe, S. J. The effect of pulsed electric fields on biological cells: Experiments and applications. IEEE Trans Plasma Sci. 1997,25(2):284~292
134. Peleg, M. A model of microbial survival after exposure to pulse electric fields. J Sci Food Agric. 1995,67(1):93~99
135. Hulsheger, H., Pottel, J. and Niemann, E. G. Killing of bacteria with electric pulses of high field strength. Radiat Environ Biophys. 1981,20:53~65 5.
136. Barbosa-Cánovas, G. V., Gongora-Nieto, M. M., Pothakamury, U. R., Swanson, B. G. Preservation of foods with pulsed electric fields. 1999,1-9, 76~107, 108-155. Academic Press Ltd. London.
137. Love, P. Correlation of fourier transforms of pulsed electric field waveform and microorganism inactivation. IEEE Transactions on Dielectrics and Electrical Insulation. 1998,5(1):142~147
138. Jayaram, S., Castle, G. S. P. and Margaritis, A. Kinetics of sterilization of Lactobacillus brevis cells by the application of high voltage pulses. Biotechnol Bioeng. 1992,40(11):1412~1420
139. Qin, B.-L., Chang, F.-J., Barbosa-Cánovas, G. V. and Swanson, B. G.Nonthermal inactivation of S. cerevisiae in apple juice using pulsed electric fields. Lebensm Wiss Technol. 1995,28(6):564~568
140. Pothakamury, U. R., Vega, H., Zhang, Q. H., Barbosa-Cánovas, G. V. and Swanson, B. G. Effect of growth stage and processing temperature on the inactivation of E. coli by pulsed electric fields. J Food Protect. 199659(11):1167~1171
141. Jacob, H. E., Forster, W. and Berg, H. Microbial implications of electric field effects. II. Inactivation of yeast cells and repair of their cell envelope. Z. Allg. Microbial 1981,21(3):225~232
142. Gaskova, D., Sigler, K., Janderova, B. and Plasek, J. Effeck of high-voltage electric pulses on yeast cells: Factors influencing the killing efficiency. Bioelectrochem Bioenergetics. 1996,39:195~202.
143. 赵武奇,殷涌光等.高压脉冲电场杀菌系统设计与试验. 农业机械学报,2002,33(3):67~69
144. Jayaram S, Castle G S P, et al. The effects of high field pulse and liquid medium conductivity on survivability of Lactobacillus brevis[J].Appl Microbiol Biotechnol ,1993,40:119~122
145. U.S.FDA Center for Food Safety and Applied Nutrition. Kinetics of Microbial Inactivation for Alternative Food Processing Technologies: Pulsed Electric Fields.2000,June 2.
146. Castro, A. J., Barbosa-Cánovas, G. V. and Swanson, B. G. Microbial inactivation of foods by pulsed electric fields. J Food Process Pres. 1993,17:47~73
147. Zimmermann, U. Electrical breakdown, electropermeabilization and electrofusion. Rev Physiol Biochem Pharmacol. 1986,105:175~25
148. 雷鸣书,黄勇,蒋正宇. 脉冲磁场灭菌.食品科学,1993,(12):55~56
149. Sato M, Clements S. Formation of chemical species and their effects on microorganisms using a pulsed high-voltage discharge in water. TEEE Transactions on industry Applications, 1996,32(12):106
150. 李胜利等.脉冲电晕对印染废水脱色效果的实验研究.环境科学,1996,17(1):13~1
151. 邵鹏.电场作用下细胞膜电压时空特性的研究. 天津大学硕士学位论文,2002 年
152. 储茂泉,古宏晨,刘国杰.中草药浸提过程的动力学模型.中草药,2000,31(7):504~506
153. 戴干策, 任德呈, 范自晖.传递现象导论 1 北京: 化学工业出版社, 1996.
2. 王春青,吕树臣,李荣权. 中国林蛙的开发与利用[J]. 当代畜牧,2002,8:24-25.
3. 崔桂友. 哈士蟆油的形态结构观察和化学成分测定. 生物学杂志,2000,17(1):27~28.
4. 金莉莉,刘德文,范文今,等. 中国林蛙卵、卵油及输卵管对红细胞 SOD 活性的影响. 中国公共卫生,2002,18(5):607.
5. 崔贞玉,王琳,张琨,等. 林蛙油对老年雌性大鼠脂质过氧化的影响. 环境与职业医学,2002,19(3):204.
6. 于勇,刘阳,范文今,等. 林蛙油耐缺氧与调节血脂的作用. 环境与职业医学,2002,19(3):204~205.
7. 李成义,孙文伟,姜熙罗,等. 蛤士蟆卵油化学成分分析. 中国药学杂志,1994,29(12):712~713.
8. 李成义,李静波,李广文,等. 吉林产蛤士蟆卵油中微量元素含量测定. 中药材,1991,14(1):16~17.
9. 卢立明,宋少江,徐绥绪,等. 蛤士蟆卵油化学成分研究. 中国药物化学杂志,2001,11(4):224~225.
10. 彭飞,徐峰,刘彬,等. 中国林蛙卵油对高脂血症大鼠降脂作用的观察. 医学理论与实践,2003,16(9):996~997.
11. 彭飞,于虹,徐峰,等. 中国林蛙卵油的中枢抑制作用. 沈阳药科大学学报,2002,19(3):204~207.
12. 杜景新,王丽,燕杰. 中国林蛙各部位氨基酸含量的分析. 人参研究,2003,3:40~42.
13. 张晓静,刘会东.植物多糖提取分离及药理作用的研究进展.时珍国医国药,2003,14(8):494~497
14. 魏培莲.微生物胞多糖研究进展.浙江科技学院学报,2003,14(2):8~12
15. 骆传环,黄荣清,刘曙晨.海星多糖的分子量测定.军事医学科学院院刊,2002,26(2).
16. 樊绘曾、陈菊娣等.玉足海参酸性多糖的研究.药学学报,1983,18(3):203~208
17. 许东晖,王兵,许实波,等.鲍鱼多糖对荷瘤小鼠腹腔巨噬细胞迟发型超敏反应的作用.中药材,1999,22(2):88
18. 胡健饶,曹明富.三角帆蚌多糖抑瘤作用的实验研究.中国现代应用药学杂志,2003,20(1):11~13
19. 窦昌贵,黄芳,黄罗生,等.文蛤多糖抗癌免疫药理作用的研究.中国海洋药物,1999,18(2):15
20. 唐巍然,闻杰,于晓红,等.鹿茸多糖对小鼠免疫功能的影响.中医药信息,1998,15(6):43
21. 黄林清,周世文,张诗平,等.蚕蛹多糖对小鼠免疫功能的影响.解放军药学学报,2002,18(1):11~13.
22. 邱鹏新,黎明涛,等.黑海参多糖对 β-淀粉样蛋白诱导的皮质神经元凋亡的保护作用.中草药,2000,31(4):271~274
23. 吕昌龙,赫甡,刘北星,等.乌贼墨及其提取物的免疫刺激作用.中国海洋药物,1999,18(2):32
24. 李东霞,李德全,张双全.鲨鱼软骨多糖的理化性质及其与 DNA 分子相互作用的研究.海洋科学,2000,24(5):40~43
25. 赵本树,杜晓东,吕金梁,等.鲨鱼软骨酸性多糖对小鼠的降脂作用.中国药理学通报,1995,11(3):259.
26. 高存记,李家增,彭林,等.刺参酸性粘多糖对纤维蛋白凝胶结构及其溶解性的影响.中华血液学杂志,1996,17(9):458
27. 王大为,张艳荣,沙坤.中国林蛙皮多糖类天然保湿因子的提取与鉴定.吉林农业大学学报,2002,24(6):99~102,106
28. 钱晋,过鑫昌,王学锋,等.玉足海参酸性粘多糖对冠心病患者血凝、血液粘度及血脂的影响.上海医学,1997,20(6):342
29. 钦传光,黄开勋,徐辉碧.泥鳅多糖的免疫作用研究.中国药学杂志,2002,37(8):
588~591.
30. 钦传光,周军,赵文,等.泥鳅多糖清除活性氧自由基和保护 DNA 链的作用.生物化学与生物物理学报,2001,33(2):215~218.
31. 钦传光,黄开勋,徐辉碧.泥鳅及其黏液抗炎作用的实验研究[J].中国药学杂志,2000,35(2):846~847.
32. 钦传光,黄开勋,徐辉碧.泥鳅多糖对试验型糖尿病小鼠血糖血脂的影响[J].中国药理学与毒理学杂志,2002,16(2):124~127.
33. 苏秀榕,娄永江,等.海参的营养成分及海参多糖的抗肿瘤活性的研究.营养学报,2003,25(2):181~183
34. 丁素菊,郑惠民,史荫绵.中性粘多糖对体外超氧阴离子自由基的研究.第二军医大学学报,1994,15(5):464.
35. 李志军,李八方,等.五种海洋多糖抗疲劳作用的初步研究.中国海洋药物,2003,2:53~56
36. 张彦民,李宝才,朱利平,等.多糖化学及其生物活性研究进展.昆明理工大学学报(理工版),2003,28(3):140~149.
37. 刘天龙,许剑琴.多糖现代研究及应用进展.中国兽医杂志,2004,40(3):24~26.
38. 马宝瑕,陈新,邓军娥.中药多糖研究进展.中国医院药学杂志,2003,23(6):360~362
39. 沈鸣,陈建伟.氨基多糖的药理研究进展.上海医药,2001,22(6):268~269.
40. 王琪琳,王海仁.海洋生物多糖药用功能的新进展.生物学通报,2002,37(7):12~13.
41. Murray HG,et al. Scheur PJ (ed):Bioorganic Marine Chemistry I,Springer-Kerlag,Berlin Heidelberg,1987,107~121.
42. Umezawa H, ckami Y, okurasawa S etal. Marinactan antitumor polysaccharide produced by marine bacteria [J]. Antibiot, 1983, 36: 371~376.
43. Nakashima H, kido Y. kokayashi N, et al. Antiretroviral activity in a marine Red alga:reverse extract of schizymenia pacifica [J]. J cancer Red Clin oncol, 1987,113(5):413-419.
44. Cassaro C M F, et. Distribution of sulphated mucopolysaccharides in invertebrates. J Biochem, 1977,252:2254~2259.
45. Ovodov Y S. et al. Polysaccharides of some Japan Sea invertebrates. Comp Biochem physiol, 1969, 29:1093~1099.
46. Rianchini P, et al. Fraction and identification of heparin and other acidic mucopolysaccharides by a new discontinuous electrophoretic method. J Chromalogr, 1980,196:455~461.
47. Dietrich C P, et al. Identification of acidic mucopolysaccharides by agarose gel electrophoresis [J] Chromatogr, 1977,130:299~235.
48. Guerard F, et al., Comparative Studies on Fish Skin Mucins, Program and Abstracts. Second International Marine Biotechnology Conference (IMBC’91),1991,p29.
49. 周铭东,崔悦礼,陈静华等.贝类多糖的组成及性质研究.云南大学学报(自然科学版)1998,20(3):187~189.
50. 姚新生.天然药物化学[M].第三版.北京:人民卫生出版社,2002.53~106.
51. Quass, D. W. Pulsed electric field processing in the food industry. A status report on PEF. Palo Alto, CA. Electric Power Research Institute. CR-109742. 1997.
52. Palaniappan S,Sasfry S K. Effects of electricity on microorganisms:a review [J].J Food Process Preserv,1990,14:393~414.
53. 时兰春,王伯初. 高压电脉冲灭菌理论的研究进展.重庆大学学报,2002,25(4):144~147.
54. Sale A J H, Hamilton W A. Effects of high electric fields on microorganisms [J].Biochim Biophys Acta, 1967,(148):781~788.
55. Hulsheger H, Niemann E G. Lethal effects of high-voltage pulses on E.coli K12 [J].Radiat Environ Biophys,1980,(22):149~162.
56. Zimmermann U . Electric breakdown , electropermeabilization and electrofusion Rev[J].Phys Biochem Phamacol,1986,105:196~256.
57. Vego-Mercado H, Pothakamury U R, Chang F J, et al. Inactivation of E.coli by combining pH, ionic strength and pulsed electric fields hurdles[J]. Food Res Int, 1996,29(2):117~121.
58. Tsong T Y. Electroporation of cell membranes [J]. Biophys J. 1991,60:297~306.
59. Macgrecor S J,Farish 0,Fouracre R,et a1.Inactivation of pathogenic and spoilage microorganisms in a test liquid using pulsed electric fields [J].IEEE transactions on plasma science,2000,28(1):144~149.
60. Pothakamury, U. R., Monsalve-Gonzalez, A., Barbosa-Cánovas, G. V. and Swanson, B. G.Inactivation of Escherichia coli and Staphylococcus aureus in model foods by pulsed electric field technology. Food Res Int. 1995.28(2):167~171.
61. Martin-Belloso, O., Qin, B. L., Chang, F. J., Barbosa-Cánovas, G. V. and Swanson, B. Inactivation of Escherichia coli in skim milk by high intensity pulsed electric fields. J Food Process Eng. 1997 20:317~336.
62. Zhang, Q. H., Qin, B.-L., Barbosa-Cánovas, G. V. and Swanson, B. G. Inactivation of E. coli for food pasteurization by high-strength pulsed electric fields. J Food Process Preserv. 1995, 19(2):103~118.
63. Qin, B., Pothakamury, U. R., Vega, H., Martin, O., Barbosa-Cánovas, G. V. and Swanson, B. G. Food pasteurization using high intensity pulsed electric fields. J Food Technol. 1995,49(12):55~66.
64. Vega-Mercado, H., Martin-Belloso, O., Qin, B.-L., Chang, F.-J., Gongora-Nieto, M. M., Barbosa-Cánovas, G. V. and Swanson, B. G. Non-thermal food preservation: pulsed electric fields. Trends Food Sci Technol. 1997,8(5):151~157.
65. Ma, L., Chang, F. J. and Barbosa-Cánovas, G. V. Inactivation of E. coli in liquid whole eggs using pulsed electric fields technologies. New frontiers in food engineering. Proceedings of the Fifth Conference of Food Engineering. American Institute of Chemical Engineers. 1997.216~221.
66. Qin, B.-L., Barbosa-Cánovas, G. V., Swanson, B. G. and Pedrow, P. D. Inactivating microorganism using a pulsed electric field continuous treatment system. IEEE Trans Indus Applic. 1998,34(1):43~49.
67. Zhang, Q. H., Qiu, X. and Sharma, S. K. Recent development in pulsed electric field processing. Washington, DC. National Food Processors Association. New Technologies Yearbook. 1997,31~42.
68. Dunn, J. E. and Pearlman, J. S. Methods and apparatus for extending the shelf-life of fluid food products. Maxwell Laboratories, Inc. U. S. Patent 1987,4,695,472.
69. Sitzmann, V. High voltage pulse techniques for food preservation. G. W. Gould. New methods for food preservation. London, UK. Blackie Academic and Professional. 1995,236~252.
70. Grahl, T., Sitzmann, W. and Markl, H. Killing of microorganisms in fluid media by high-voltage pulses. DECHEMA Biotechnology Conferences. 1992,675~679.
71. Ho, S. Y., G.S., M., Cross, J. D. and Griffiths, M. W. Inactivation of Pseudomonas fluorescens by high voltage electric pulses. J Food Sci. 1995,60(6):1337~1343.
72. Ho, S. Y., Mittal, G. S. and Cross, J. D. Effects of high field electric pulses on the activity of selected enzymes. J Food Eng. 1997,31(1):69~84.
73. Marquez, V. O., Mittal, G. S. and Griffiths, M. W. Destruction and inhibition of bacterial spores by high voltage pulsed electric field. J Food Sci. 1997,62(2):399~401,409
74. Mittal, G. S. and Choundry, M. Pulsed electric field sterilization of waste brine solution.Proceedings of the Seventh International Congress on Engineering and Food. Brighton Center, UK. 1997,C13~C16.
75. Hulsheger, H., Pottel, J. and Niemann, E. G. Electric field effects on bacteria and yeast cells. Radiat Environ Biophys. 1983,22:149~162
76. Grahl, T. and Maerkl, H. Killing of microorganisms by pulsed electric fields. Applied MicrobiolBiotechnol. 1996,45(1/2):148~157
77. 曾新安,陈勇主编.脉冲电场非热灭菌技术.中国轻工业出版社,2005
78. Qin, B. L., Zhang, Q., Barbosa-Cánovas, G. V., Swanson, B. G. and Pedrow, P. D. Inactivation of microorganisms by pulsed electric fields with different voltage waveforms. IEEE Trans Dielec Insul. 1994,1(6):1047~1057
79. Qin, B.-L., Zhang, Q. H., Barbosa-Cánovas, G. V., Swanson, B. G. and Pedrow, P. D. Pulsed electric field treatment chamber design for liquid food pasteurization using a finite element method. Transactions of the ASAE. 1995,38(2):557~565
80. Mizuno, A. and Hori, Y. Destruction of living cells by pulsed high-voltage application. IEEE Trans Ind Appl. 1991,24(3):387~394
81. Zhang, Q. H., Monsalve-Gonzalez, A., Barbosa-Cánovas, G. V. and Swanson, B. G. Inactivation of E. coli and S. cerevisiae by pulsed electric fields under controlled temperature conditions. Transactions of the ASAE. 1994,37(2):581~587
82. Zhang, Q. H., Chang, F.-J. and Barbosa-Cánovas, G. V. Inactivation of microorganisms in a semisolid model food using high voltage pulsed electric fields. Lebensm Wiss Technol. 1994,27(6):538~543
83. Fernandez-Molina, J. J., Barkstrom, E., Torstensson, P., Barbosa-Cánovas, G. V. and Swanson, B. G. Shelf-life extension of raw skim milk by combining heat and pulsed electric fields. Food Res Int. 1999.
84. Calderon-Miranda, M. L. Inactivation of listeria inocua by pulsed electric fields and nisin. Pullman, WA. Washington State University. 1998.
85. Qin, B.-L., Barbosa-Cánovas, G. V., Swanson, B. G. and Pedrow, P. D. Inactivating microorganism using a pulsed electric field continuous treatment system. IEEE Trans Indus Applic. 1998,34(1):43~49
86. Liu, X., Yousef, A. E. and Chism, G. W. Inactivation of Escherichia coli O157:H7 by the combination of organic acids and pulsed electric field. J Food Safety. 1997,16(4):287~299
87. Simpson, M. V., Barbosa-Cánovas, G. V. and Swanson, B. G. The Combined inhibitory effect of lysozyme and high voltage pulsed electric fields on the growth of Bacillus subtilis spores. IFT Annual Meeting: Book of Abstracts. 1995,267.
88. Pagan, R., Esplugas, S., Gongora-Nieto, M. M., Barbosa-Cánovas, G. V. and Swanson, B. G. Inactivation of Bacillus subtilis spores using high intensity pulsed electric fields in combination with other food conservation technologies. Food Scie Technol Int. 1998,4(1):33~44
89. Castro, A. J. Pulsed electrical field modification of activity and denaturation of alkaline phosphatase. Food Science and Human Nutrition. Pullman, WA. Washington State University. 1994.
90. Vega-Mercado, H., Powers, J. R., Barbosa-Cánovas, G. V. and Swanson, B. G. Plasmin inactivation with pulsed electric fields. J Food Sci. 1995,60(5):1143~1146
91. Yeom,H.W.,Zhang,Q.H.,Dunne,C.P.Inactivation of papain by pulsed electric fields in a continuous system. Food Chemistry.1999,67,53~59.
92. Barsotti, L.,Dumay, E.,Mu, T.H.,Fernandez Diaz,M.D.,Cheftel,J.C.Effects of high voltage electric pulses on proteinbased food constituents and structures. Trends in food Science and Technology. 2001,12(3):136~144
93. 钟葵,孙志健等,高压脉冲电场对辣根过氧化物酶活性及构象的影响效果。中国食品学报,2005,5(1):31~35
94. Joaquin Giner, Vicente Gimeno, Alexandre Eapachs, et al. Inhibition of tomato Licopersicon esculentum Mill. Pectin methylesterase by pulsed electric fields. Innovative Food Science and Emerging Technologies. 2000,1:57~67.
95. S.Min, S.K.Min, et al.Inactivation kinetics of tomato juice lipoxygenase by pulsed electric field. Food Engineering Physical Properties. 2003,68(6):1995~2001.
96. Giner,J.,Rauret-Arino,A.,et al. Inactivation of polyphenoloxidase by pulsed electric fields. In proceedings IFT Annual Meeting, 1997,19,Orlando, USA.
97. Seacheol Min, Z.Tony Jin,and Q.Howard Zhang.Commercial scale PEF processing of tomato juice.J.Agric.Food Chem. 2003,51:3338~3344.
98. Z.Tony Jin and Q.Howard Zhang. PEF inactivation of microorganisms and preservation of quality of cranberry juice. Jounal of Food Processing Preservation.1999,23,481~497.
99. L.Barsotti,P.Merle and J.C.Cheftel. Food processing by PEF I:Physical aspects.Ⅱ .Biological aspects Food Rev. Int.1999,15(2):163~213.
100. Dunn, J. Pulsed light and pulsed electric field for foods and eggs. Poul Sci. 1996,75(9):1133~1136
101. Fernandez-Molina, J. J., Barkstrom, E., Torstensson, P., Barbosa-Cánovas, G. V. and Swanson, B. G. Shelf-life extension of raw skim milk by combining heat and pulsed electric fields. Food Res Int. 1999.
102. Reina, L. D., Jin, Z. T., Yousef, A. E. and Zhang, Q. H. Inactivation of Listeria monocytogenes in milk by pulsed electric field. J Food Protect. 1998,61(9):1203~1206
103. Knorr, D., Geulen, M., Grahl, T. and Sitzmann, W. Food application of high electric field pulses. Trends Food Sci Technol. 1994, 5:71~75
104. 方胜,孙学兵,陆守道.利用高压脉冲电场加速冰解冻的试验研究.北京工商大学学报,2003,21(4):43~53
105. Ngo Vu. Electric field effects on drop size and terminal velocity in liquid-liquid systems. AIChEJ. 1986,32(10):1739~1742
106. Costerton J W, Irvin R T, Cheng K J. The bacterial glycocalyx in nature and disease. Annu Rev Microbiol, 1999,35:299~327
107. 刘学湘,陈建伟. 梅花参中梅花参多糖的提取工艺研究[J]. 南京中医药大学学报,2003,19(3):161~163
108. 李熙灿,曾和平,杨世柱. 黑海参多糖组成的研究[J]. 广州化学,1998(1):17~21.
109. 孙兴权,胡耀辉,付蕾,于晓斌.中国林蛙头中抗凝物质的分离纯化及抗凝活性研究[J].吉林农业大学学报,2000,22(3):100~103.
110. Kennedy JF, Proteoglycans, Elsevier, Amsterdam, 1979
111. Danishefsky I, et al., J Biochem, 1966, 241:143
112. 樊绘曾. 海洋药物,1983,1:40
113. Karamanos NK, et al. Biochem Biophys Acta,1988,966(1):36
114. 王长云,管华诗.氨基多糖的提取、分离和分析测定方法及其研究进展.中国海洋药物,1996,1:24~31
115. 张惟杰.糖复合物生化研究技术[M].杭州:浙江大学出版社,1999,128.
116. Fenichel RL. Immune modulation agents and their mechanism[M]. New York:Marcel Dekker inc, 1984,409.
117. 张豁中,温玉麟. 动物活性成分化学[M].天津:天津科学技术出版社,1999,1~186.
118. 马常夫. 哈士蟆的年龄鉴定与种群结构的初步研究[J]. 东北师范大学学报(自然科学版),1985(1):81~89.
119. 沈鸣,陈建伟. 糙海参多糖纯化工艺优选及其产物的药理活性比较[J]. 中国海洋药物,2003(1):1~4.
120. 任露泉编著.试验优化设计与分析(第 1 版).长春:吉林科学技术出版社,2001,5
121. 涂国云,卜宗式,杜昱光. 酶法提取竹荪深层发酵菌丝体多糖的研究. 中国食用菌,1998,17(1),36~38
122. 林宇野,杨虹. 酶法提取多糖的研究. 食品与发酵工业,1995(1):13~17
123. 陈哲超,林宇野. 碱解、酶解复合法提取香菇多糖的研究. 生物工程进展,1995,15(1):47~50
124. 韩慧敏,谢安,罗杰英. 海参多糖提取工艺研究[J]. 湖南中医杂志,2000,16(1):56~57.
125. 王长云,管华诗,刘树青. 扇贝糖胺聚糖的琼脂糖凝胶电泳分析[J]. 中国海洋药物,1996,2:12~14
126. 孙兴权,付蕾,曹云龙,胡耀辉.林蛙多糖(TCPS)提取工艺的初步研究 [J].吉林农业大学学报,2001,23(4):108~110
127. 冯年平,郁威主编.中药提取分离技术原理与应用.中国医药科技出版社,2005 年
128. 赵兵,苏立民.电场絮凝提取碱性蛋白酶的研究.化工冶金,1991,12(2):143~149
129. 赵兵,苏立民.电场絮凝分离提取 α-淀粉酶.化工学报,1991,(1):109~113
130. Zhang, Q. H., Monsalve-Gonzalez, A., Barbosa-Cánovas, G. V. and Swanson, B. G. Inactivation of E. coli and S. cerevisiae by pulsed electric fields under controlledtemperature conditions. Transactions of the ASAE. 1994,37(2):581~587
131. Dunne, C. P., Dunn, J., Clark, W., Ott, T. and Bushnell, A. H. Application of high energy electric field pulses to preservation of foods for combat rations. Science and Technology for Force XXI. Department of the Army. Norfolk, Virginia. 1996, June 24~27. 7.
132. Jeyamkondan, S., Jayas, D. S. and Holley, R. A. Pulsed electric field processing of foods: a review. J Food Protect. 1999,62(9):1088~1096
133. Schoenbach, K. H., Peterkin, F. E., Alden, R. W. and Beebe, S. J. The effect of pulsed electric fields on biological cells: Experiments and applications. IEEE Trans Plasma Sci. 1997,25(2):284~292
134. Peleg, M. A model of microbial survival after exposure to pulse electric fields. J Sci Food Agric. 1995,67(1):93~99
135. Hulsheger, H., Pottel, J. and Niemann, E. G. Killing of bacteria with electric pulses of high field strength. Radiat Environ Biophys. 1981,20:53~65 5.
136. Barbosa-Cánovas, G. V., Gongora-Nieto, M. M., Pothakamury, U. R., Swanson, B. G. Preservation of foods with pulsed electric fields. 1999,1-9, 76~107, 108-155. Academic Press Ltd. London.
137. Love, P. Correlation of fourier transforms of pulsed electric field waveform and microorganism inactivation. IEEE Transactions on Dielectrics and Electrical Insulation. 1998,5(1):142~147
138. Jayaram, S., Castle, G. S. P. and Margaritis, A. Kinetics of sterilization of Lactobacillus brevis cells by the application of high voltage pulses. Biotechnol Bioeng. 1992,40(11):1412~1420
139. Qin, B.-L., Chang, F.-J., Barbosa-Cánovas, G. V. and Swanson, B. G.Nonthermal inactivation of S. cerevisiae in apple juice using pulsed electric fields. Lebensm Wiss Technol. 1995,28(6):564~568
140. Pothakamury, U. R., Vega, H., Zhang, Q. H., Barbosa-Cánovas, G. V. and Swanson, B. G. Effect of growth stage and processing temperature on the inactivation of E. coli by pulsed electric fields. J Food Protect. 199659(11):1167~1171
141. Jacob, H. E., Forster, W. and Berg, H. Microbial implications of electric field effects. II. Inactivation of yeast cells and repair of their cell envelope. Z. Allg. Microbial 1981,21(3):225~232
142. Gaskova, D., Sigler, K., Janderova, B. and Plasek, J. Effeck of high-voltage electric pulses on yeast cells: Factors influencing the killing efficiency. Bioelectrochem Bioenergetics. 1996,39:195~202.
143. 赵武奇,殷涌光等.高压脉冲电场杀菌系统设计与试验. 农业机械学报,2002,33(3):67~69
144. Jayaram S, Castle G S P, et al. The effects of high field pulse and liquid medium conductivity on survivability of Lactobacillus brevis[J].Appl Microbiol Biotechnol ,1993,40:119~122
145. U.S.FDA Center for Food Safety and Applied Nutrition. Kinetics of Microbial Inactivation for Alternative Food Processing Technologies: Pulsed Electric Fields.2000,June 2.
146. Castro, A. J., Barbosa-Cánovas, G. V. and Swanson, B. G. Microbial inactivation of foods by pulsed electric fields. J Food Process Pres. 1993,17:47~73
147. Zimmermann, U. Electrical breakdown, electropermeabilization and electrofusion. Rev Physiol Biochem Pharmacol. 1986,105:175~25
148. 雷鸣书,黄勇,蒋正宇. 脉冲磁场灭菌.食品科学,1993,(12):55~56
149. Sato M, Clements S. Formation of chemical species and their effects on microorganisms using a pulsed high-voltage discharge in water. TEEE Transactions on industry Applications, 1996,32(12):106
150. 李胜利等.脉冲电晕对印染废水脱色效果的实验研究.环境科学,1996,17(1):13~1
151. 邵鹏.电场作用下细胞膜电压时空特性的研究. 天津大学硕士学位论文,2002 年
152. 储茂泉,古宏晨,刘国杰.中草药浸提过程的动力学模型.中草药,2000,31(7):504~506
153. 戴干策, 任德呈, 范自晖.传递现象导论 1 北京: 化学工业出版社, 1996.