睑板腺癌SSTR2的表达
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摘要
目的：研究睑板腺癌SSTR2的表达情况，探讨SSTR2表达与睑板腺癌病理学特点之间的关系。
方法：收集睑板腺癌组织标本60例及正常睑板腺组织标本20例。每个标本制成5μm厚组织切片3张，一张行HE染色用于病理学分类，一张采用免疫组化法检测SSTR2的表达，一张以PBS液代替一抗作为空白对照。采集图像，利用IPP(Image—Pro Plus 6.0)分析图象，通过测图像MOD(Mean Optical Density平均光密度)值判断图片染色强度。采用SPSS13.0统计学软件进行分析比较SSTR2的表达与睑板腺癌病理学特征间的关系及正常组织组与睑板腺癌组织间的差异性。
结果：正常睑板腺组织及睑板腺癌组织中均有SSTR2的表达且均表达于细胞浆膜上。睑板腺癌组织MOD值明显大于正常睑板腺组织，差异有统计学意义(p=0.000＜0.05)。睑板腺癌不同分化程度组间MOD值存在差异，但差异无统计学意义(p=0.898＞0.05)。睑板腺癌SSTR2的表达与患者的年龄、性别、发病部位、病程、肿瘤分化程度、复发情况、转移情况等病理学特点之间存在差异，但差异无统计学意义(p均＞0.05)。
结论：正常睑板腺组织及睑板腺癌组织细胞浆膜上均有SSTR2的表达。睑板腺癌组织中SSTR2表达高于正常睑板腺组织，SSTR2的表达与睑板腺癌的分化程度无明显相关。
Purpose To detect the expression of somatostatin receptor 2 (SSTR2) ofthe specimens of meibomian gland carcinoma. To discuss the relationshipbetween the expression of SSTR2 and pathologic characteristics of thetumor.
Methods 60 specimens of meibomian gland carcinoma and 20specimens of normal meibomian gland were collected. Every specimen wasmade 3 sections, thickness of the section was 5μm. One section was stainedby HE; one section was stained by Streptavidin-Peroxidaseimmunohistochemistry for the expression of SSTR2; one section was used forblank control by using PBS substitution the first antibody. Pictures wereanalyzed by Image—Pro Plus 6.0 (IPP 6.0). The degree of stain wasdetermined by Mean Optical Density (MOD) of sections. The difference inexpression of SSTR2 between carcinoma of meibomian gland tissues andnormal meibomian gland tissues was analysed by SPSS, version 13.0.
Results Expression of SSTR2 was positive both in normal meibomiangland tissues and carcinoma of meibomian gland. The distribution of SSTR2was in plasma and membrane. MOD values in carcinoma of meibomiangland tissues were higher than it in normal meibomian gland tissues. Thedifference of MOD values was significant statistical (p=0.000＜0.05). MODvalues were different in different differentiation of carcinoma of meibomiangland tissues, but the difference was not significant statistical (p=0.898＞0.05). We found no significant statistical relationship between theexpression of SSTR2 and age, sex, position, course of disease,differentiation, recurrence, metastasis (p＞0.05).
Conclusions Expression of SSTR2 was positive both in normalmeibomian gland tissues and carcinoma of meibomian gland. Thedistribution of SSTR2 was in plasma and membrane. Expression of SSTR2in carcinoma of meibomian gland tissues was higher than expression ofSSTR2 in normal meibomian gland tissues. In expression of SSTR2, wefound no relationship in differentiated degree of meibomian gland carcinoma.
方法：收集睑板腺癌组织标本60例及正常睑板腺组织标本20例。每个标本制成5μm厚组织切片3张，一张行HE染色用于病理学分类，一张采用免疫组化法检测SSTR2的表达，一张以PBS液代替一抗作为空白对照。采集图像，利用IPP(Image—Pro Plus 6.0)分析图象，通过测图像MOD(Mean Optical Density平均光密度)值判断图片染色强度。采用SPSS13.0统计学软件进行分析比较SSTR2的表达与睑板腺癌病理学特征间的关系及正常组织组与睑板腺癌组织间的差异性。
结果：正常睑板腺组织及睑板腺癌组织中均有SSTR2的表达且均表达于细胞浆膜上。睑板腺癌组织MOD值明显大于正常睑板腺组织，差异有统计学意义(p=0.000＜0.05)。睑板腺癌不同分化程度组间MOD值存在差异，但差异无统计学意义(p=0.898＞0.05)。睑板腺癌SSTR2的表达与患者的年龄、性别、发病部位、病程、肿瘤分化程度、复发情况、转移情况等病理学特点之间存在差异，但差异无统计学意义(p均＞0.05)。
结论：正常睑板腺组织及睑板腺癌组织细胞浆膜上均有SSTR2的表达。睑板腺癌组织中SSTR2表达高于正常睑板腺组织，SSTR2的表达与睑板腺癌的分化程度无明显相关。
Purpose To detect the expression of somatostatin receptor 2 (SSTR2) ofthe specimens of meibomian gland carcinoma. To discuss the relationshipbetween the expression of SSTR2 and pathologic characteristics of thetumor.
Methods 60 specimens of meibomian gland carcinoma and 20specimens of normal meibomian gland were collected. Every specimen wasmade 3 sections, thickness of the section was 5μm. One section was stainedby HE; one section was stained by Streptavidin-Peroxidaseimmunohistochemistry for the expression of SSTR2; one section was used forblank control by using PBS substitution the first antibody. Pictures wereanalyzed by Image—Pro Plus 6.0 (IPP 6.0). The degree of stain wasdetermined by Mean Optical Density (MOD) of sections. The difference inexpression of SSTR2 between carcinoma of meibomian gland tissues andnormal meibomian gland tissues was analysed by SPSS, version 13.0.
Results Expression of SSTR2 was positive both in normal meibomiangland tissues and carcinoma of meibomian gland. The distribution of SSTR2was in plasma and membrane. MOD values in carcinoma of meibomiangland tissues were higher than it in normal meibomian gland tissues. Thedifference of MOD values was significant statistical (p=0.000＜0.05). MODvalues were different in different differentiation of carcinoma of meibomiangland tissues, but the difference was not significant statistical (p=0.898＞0.05). We found no significant statistical relationship between theexpression of SSTR2 and age, sex, position, course of disease,differentiation, recurrence, metastasis (p＞0.05).
Conclusions Expression of SSTR2 was positive both in normalmeibomian gland tissues and carcinoma of meibomian gland. Thedistribution of SSTR2 was in plasma and membrane. Expression of SSTR2in carcinoma of meibomian gland tissues was higher than expression ofSSTR2 in normal meibomian gland tissues. In expression of SSTR2, wefound no relationship in differentiated degree of meibomian gland carcinoma.
引文
1 戴京，孙宪丽，李彬等．8673例眼附属器增生性病变及肿瘤组织的病理分析．中华眼科杂志，1999；35(4)：258-261
2 Kass LG, Hornblass A. Sebaceous carcinoma of the ocular adnexa. Surv ophthalmol, 1989; 33: 477-490
3 Jerry A. Shields, Hankan Demirci, Brian P. Marr, et al. Sebaceous carcinoma of the ocular region: A review. Survey ophthalmology, 2005 Mar-April 2; 50: 103-122
4 立彦、夏伟、瞿卫等．生长抑素及其受体的研究与临床应用现状．国外医学临床生物化学与检验学分册．2004；25(6)：529-535
5 Yamada Y, Post SR, Wang K, et al. Cloning and functional characterization of a family of human and mouse somatostatin receptors expressed in brain, gasteointestinal tract and kidney. Proc Natl Acad Sci USA, 1992; 89: 251-255
6 Patel YC. Somatostain and its receptor family. Frontiers Neuroendocrinol, 1999; 20: 157-159
7 汤钊酋等．现代肿瘤学．上海：复旦大学出版社；2003；1063-1064
8 武忠弼，杨光华．中华外科病理学．北京：人民卫生出版社；2002；2085-2086．
9 Bell GI, Yasuda K, Kong H, et al. Molecular biology of somatostatin receptor. Ciba Found Symp. 1995; 190: 65-88
10 David M. Cook, Marie B. Cook. Managing acromegaly with somatostatin analogs. The endocrinologist. 2006; 16(2): 100-108
11 Lahlou M, Guillermet J, Hortala M, et al. Molecular signaling of somatostatin receptors. Ann N Y Acad Sci. 2004; 1014: 121-131
12 Rocheville M, Lange DC, Kumar U, et al. Receptors for dopamine and somatostatin: formation of hetero-oligomers with enhanced functional activity. Science. 2000; 288: 154-157
13 Reubi JC, Laissue JA, Waser B, et al. Immunohistochem ical detect ion of somatostatin SSTR2a receptors in the lymphatic, smooth muscular, and peripheral nervous system s of the human gastrointestinal tract: facts and artifacts. J Clin Endo Metab, 1999; 84: 2942
14 Schulz S, Schmitt J, Quednow C, et al. Immunohistochemical detection of somatostatin receptors in human ovarian tumors. Gynecol Oncol, 2002, 84(2): 235-240
15 邢兰英，刘星，史玉霞．SSTR2在上皮性卵巢肿瘤中的表达及意义．陕西医学杂志．2006：35(2)：131-133
16 刘与友，黄种心，邱成志，涂远荣．生长抑素受体亚型在食管癌的表达及意义．福建医科大学学报．2005；39(4)：376-378
17 李小毅，唐伟松，陈原稼，陈元方．生长抑素2型受体在大肠癌中的表达．中国普外基础与临床杂志．2004；11(4)：347-350
18 邱成志，黄种心，王川，朱世泽，吴友谊，邱建龙．生长抑素受体亚型在界直肠癌组织中的表达．中华实验外科杂志．2005；22(4)：442-444
19 迟志宏，郑文尧，梁浩，孙晓华．人胃癌组织中生长抑素2型受体表达的初步研究．中国肿瘤临床与康复，2001；8(2)：18-19
1 Krulich L, Dhariwal AP, McCann SM. Stimulatory and inhibitory effects of purified hypothalamic extracts on growth hormone release from rat pitvitro. Endocrinology. 1968;83:783-790
2 Brazeau P, Vale W, Burgus R, et al. Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science. 1973;179:77-79
3 Benuck M, Marks N. Differences in the degradation of hypothalamic releasing factors by rat and human serum . Life Sci. 1976; 19:1271-1276
4 Samols E, Stagner J. Islet somatostatin-microvascular, paracrine and pulsatile regulation. Metabolism. 1990;39:55-60
5 Patel YC , Liu J, Galanopoulou A, et al. Production, action, and degradation of somatostatin in:Jefferson LS ,Cherrington AD, Goodman ,HM, eds. Handbook of physiology: Section 7 The Endocrine System. Oxford Uuniversity Press;2001:267-302
6 Montminy MR, Low MJ, Tapia-Araancibia L, et al. Cyclic AMP regulates somatostatin mRNA accumulation in primary diencephalic cultures and in transfeced fibroblast cells. J Neurosci. 1986;6:1171-1176
7 Bruns C, Lewis I, Briner U, et al. S0M230: a novel somatostain peptidomimetic with broad somatotropin release inhibiting factor receptor binding and a unique antisecretory profile. Eur J Endocrinol. 2002;146:707-716.
8 Bell GI, Yasuda K, Kong H, et al. Molecular biology of somatostatin receptor. Ciba Found Symp.1995;190:65-88
9 David M. Cook, Marie B. Cook. Managing acromegaly with somatostatin analogs. The endocrinologist. 2006; 16(2): 100-108
10 Lahlou M, Guillermet J, Hortala M, et al. Molecular signaling of somatostatin receptors. Ann N Y Acad Sci. 2004; 1014: 121-131
11 Rocheville M, Lange DC, Kumar U, et al. Receptors for dopamine and somatostatin: formation of hetero-oligomers with enhanced functional activity. Science. 2000; 288: 154-157
12 Bell GI, Reisine T. Molecular biology of somatostatin receptors. Trends Neurosci. 1993; 16: 34-38
13 陈绪军，艾中立，刘志苏．生长抑素类似物的抗肿瘤作用机制．国外医学 生理、病理科学与临床分册．2000；20(3)：235-237
14 Zalatnai A, Pogany V. Apoptosis induction and phosphorylation state in human pancreatic carcinoma xenografts following octreotide treatment. Anticancer Res, 2001; 21: 477-480
15 Zalatnai A, Szegedi Z, Bocsi J. Flow cytometric evidence of apoptosis in human pancreatic cancer xenografts treated with Sandostatin. Anticancer Res. 2000, 20: 1663-1666
16 Zhao B, Zhao H, Zhao N, et al. Cholangiocarcinoma cells express somatostatin receptor subtype 2 and respond to octreotide treatment. J Hepatobiliary Pancreat Surg. 2002; 9(4): 497-502
17 颜登国，区庆嘉，李江涛．原发性肝癌生长抑素受体mRNA表达的研究．中华普通外科杂志，2003；18：55-56
18 瞿卫，王自正，王书奎，王峰．胃癌生长抑素受体mRNA表达的研究．中华肿瘤防治杂志．2006；13(7)：498-500
19 邢兰英，刘星，史玉霞．SSTR2在上皮性卵巢肿瘤中的表达及意义．陕西医学 杂志．2006；35(2)：131-133
20 代远斌，曾希志，向志．人类大肠癌SSTR2mRNA表达及其与细胞增殖相关性研究．第三军医大学学报．2005；27(23)：2363-2365
21 李小毅，唐伟松，陈原稼，陈元方．生长抑素2型受体在大肠癌中的表达．中国普外基础与临床杂志．2004；11(4)：347-350
22 Rochaix P, Delesque N, Esteve JP, et al. Gene therapy for pancreatic carcinoma: local and distant antitumor effects after somatostatin receptor sst2 gene transfer. Hum Gene Ther, 1999; 10(6): 995-1008
23 Klisovic DD, Dorisio MS, Katz SE, et al. Somatostatin receptor gene expression in human ocular tisses: RT-PCR and immunohistochemical study. Invest Ophthalmol Vis Sci, 2001, 42(10): 2193-2201
24 Karch AM. 1996 Nursing Drug Guide. Philadephia: JB Lippincott Company: 1996; 812-814
25 Smith L E, Kopchick KK, Chen W, et al. Essential role of growth hormone in ischemia-induced retinal neovascularization. Science. 1997, 276(5319): 1076
26 Luo Q, Peyman GA, Conway MD, et al. Effect of a somatostatin analog (Octreotide Acetate) on the growth of retinal pigment epitheial cells in culture. Curr Eye Res, 1996, 15(9): 909
27 戴京，孙宪丽，李彬，郑邦和，胡士敏，李辽青等．8673例眼附属器增生性病变及肿瘤组织的病理分析[J]．中华眼科杂志 1999；35(4)：258-261．
2 Kass LG, Hornblass A. Sebaceous carcinoma of the ocular adnexa. Surv ophthalmol, 1989; 33: 477-490
3 Jerry A. Shields, Hankan Demirci, Brian P. Marr, et al. Sebaceous carcinoma of the ocular region: A review. Survey ophthalmology, 2005 Mar-April 2; 50: 103-122
4 立彦、夏伟、瞿卫等．生长抑素及其受体的研究与临床应用现状．国外医学临床生物化学与检验学分册．2004；25(6)：529-535
5 Yamada Y, Post SR, Wang K, et al. Cloning and functional characterization of a family of human and mouse somatostatin receptors expressed in brain, gasteointestinal tract and kidney. Proc Natl Acad Sci USA, 1992; 89: 251-255
6 Patel YC. Somatostain and its receptor family. Frontiers Neuroendocrinol, 1999; 20: 157-159
7 汤钊酋等．现代肿瘤学．上海：复旦大学出版社；2003；1063-1064
8 武忠弼，杨光华．中华外科病理学．北京：人民卫生出版社；2002；2085-2086．
9 Bell GI, Yasuda K, Kong H, et al. Molecular biology of somatostatin receptor. Ciba Found Symp. 1995; 190: 65-88
10 David M. Cook, Marie B. Cook. Managing acromegaly with somatostatin analogs. The endocrinologist. 2006; 16(2): 100-108
11 Lahlou M, Guillermet J, Hortala M, et al. Molecular signaling of somatostatin receptors. Ann N Y Acad Sci. 2004; 1014: 121-131
12 Rocheville M, Lange DC, Kumar U, et al. Receptors for dopamine and somatostatin: formation of hetero-oligomers with enhanced functional activity. Science. 2000; 288: 154-157
13 Reubi JC, Laissue JA, Waser B, et al. Immunohistochem ical detect ion of somatostatin SSTR2a receptors in the lymphatic, smooth muscular, and peripheral nervous system s of the human gastrointestinal tract: facts and artifacts. J Clin Endo Metab, 1999; 84: 2942
14 Schulz S, Schmitt J, Quednow C, et al. Immunohistochemical detection of somatostatin receptors in human ovarian tumors. Gynecol Oncol, 2002, 84(2): 235-240
15 邢兰英，刘星，史玉霞．SSTR2在上皮性卵巢肿瘤中的表达及意义．陕西医学杂志．2006：35(2)：131-133
16 刘与友，黄种心，邱成志，涂远荣．生长抑素受体亚型在食管癌的表达及意义．福建医科大学学报．2005；39(4)：376-378
17 李小毅，唐伟松，陈原稼，陈元方．生长抑素2型受体在大肠癌中的表达．中国普外基础与临床杂志．2004；11(4)：347-350
18 邱成志，黄种心，王川，朱世泽，吴友谊，邱建龙．生长抑素受体亚型在界直肠癌组织中的表达．中华实验外科杂志．2005；22(4)：442-444
19 迟志宏，郑文尧，梁浩，孙晓华．人胃癌组织中生长抑素2型受体表达的初步研究．中国肿瘤临床与康复，2001；8(2)：18-19
1 Krulich L, Dhariwal AP, McCann SM. Stimulatory and inhibitory effects of purified hypothalamic extracts on growth hormone release from rat pitvitro. Endocrinology. 1968;83:783-790
2 Brazeau P, Vale W, Burgus R, et al. Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science. 1973;179:77-79
3 Benuck M, Marks N. Differences in the degradation of hypothalamic releasing factors by rat and human serum . Life Sci. 1976; 19:1271-1276
4 Samols E, Stagner J. Islet somatostatin-microvascular, paracrine and pulsatile regulation. Metabolism. 1990;39:55-60
5 Patel YC , Liu J, Galanopoulou A, et al. Production, action, and degradation of somatostatin in:Jefferson LS ,Cherrington AD, Goodman ,HM, eds. Handbook of physiology: Section 7 The Endocrine System. Oxford Uuniversity Press;2001:267-302
6 Montminy MR, Low MJ, Tapia-Araancibia L, et al. Cyclic AMP regulates somatostatin mRNA accumulation in primary diencephalic cultures and in transfeced fibroblast cells. J Neurosci. 1986;6:1171-1176
7 Bruns C, Lewis I, Briner U, et al. S0M230: a novel somatostain peptidomimetic with broad somatotropin release inhibiting factor receptor binding and a unique antisecretory profile. Eur J Endocrinol. 2002;146:707-716.
8 Bell GI, Yasuda K, Kong H, et al. Molecular biology of somatostatin receptor. Ciba Found Symp.1995;190:65-88
9 David M. Cook, Marie B. Cook. Managing acromegaly with somatostatin analogs. The endocrinologist. 2006; 16(2): 100-108
10 Lahlou M, Guillermet J, Hortala M, et al. Molecular signaling of somatostatin receptors. Ann N Y Acad Sci. 2004; 1014: 121-131
11 Rocheville M, Lange DC, Kumar U, et al. Receptors for dopamine and somatostatin: formation of hetero-oligomers with enhanced functional activity. Science. 2000; 288: 154-157
12 Bell GI, Reisine T. Molecular biology of somatostatin receptors. Trends Neurosci. 1993; 16: 34-38
13 陈绪军，艾中立，刘志苏．生长抑素类似物的抗肿瘤作用机制．国外医学 生理、病理科学与临床分册．2000；20(3)：235-237
14 Zalatnai A, Pogany V. Apoptosis induction and phosphorylation state in human pancreatic carcinoma xenografts following octreotide treatment. Anticancer Res, 2001; 21: 477-480
15 Zalatnai A, Szegedi Z, Bocsi J. Flow cytometric evidence of apoptosis in human pancreatic cancer xenografts treated with Sandostatin. Anticancer Res. 2000, 20: 1663-1666
16 Zhao B, Zhao H, Zhao N, et al. Cholangiocarcinoma cells express somatostatin receptor subtype 2 and respond to octreotide treatment. J Hepatobiliary Pancreat Surg. 2002; 9(4): 497-502
17 颜登国，区庆嘉，李江涛．原发性肝癌生长抑素受体mRNA表达的研究．中华普通外科杂志，2003；18：55-56
18 瞿卫，王自正，王书奎，王峰．胃癌生长抑素受体mRNA表达的研究．中华肿瘤防治杂志．2006；13(7)：498-500
19 邢兰英，刘星，史玉霞．SSTR2在上皮性卵巢肿瘤中的表达及意义．陕西医学 杂志．2006；35(2)：131-133
20 代远斌，曾希志，向志．人类大肠癌SSTR2mRNA表达及其与细胞增殖相关性研究．第三军医大学学报．2005；27(23)：2363-2365
21 李小毅，唐伟松，陈原稼，陈元方．生长抑素2型受体在大肠癌中的表达．中国普外基础与临床杂志．2004；11(4)：347-350
22 Rochaix P, Delesque N, Esteve JP, et al. Gene therapy for pancreatic carcinoma: local and distant antitumor effects after somatostatin receptor sst2 gene transfer. Hum Gene Ther, 1999; 10(6): 995-1008
23 Klisovic DD, Dorisio MS, Katz SE, et al. Somatostatin receptor gene expression in human ocular tisses: RT-PCR and immunohistochemical study. Invest Ophthalmol Vis Sci, 2001, 42(10): 2193-2201
24 Karch AM. 1996 Nursing Drug Guide. Philadephia: JB Lippincott Company: 1996; 812-814
25 Smith L E, Kopchick KK, Chen W, et al. Essential role of growth hormone in ischemia-induced retinal neovascularization. Science. 1997, 276(5319): 1076
26 Luo Q, Peyman GA, Conway MD, et al. Effect of a somatostatin analog (Octreotide Acetate) on the growth of retinal pigment epitheial cells in culture. Curr Eye Res, 1996, 15(9): 909
27 戴京，孙宪丽，李彬，郑邦和，胡士敏，李辽青等．8673例眼附属器增生性病变及肿瘤组织的病理分析[J]．中华眼科杂志 1999；35(4)：258-261．