摘要
目的
探讨在非小细胞肺癌(non-small cell lungcarcinoma,NSCLC)中PTTG、c-myc和p53的表达及其与NSCLC发生发展之间的关系。
方法
应用免疫组织化学En Vision~(TM)法测定60例NSCLC标本及27例正常肺组织中PTTG、c-myc和p53的表达。
结果
1.NSCLC组织中PTTG、c-myc和p53表达较为广泛,PTTG,c-myc阳性颗粒位于细胞核和细胞浆,p53阳性颗粒位于细胞核,着色明显强于正常肺组织。
2.PTTG、c-myc和p53在NSCLC组织中阳性表达率分别为73.33%,58.33%和53.33%;正常肺组织的阳性表达率分别为11.11%,18.52%和7.41%;PTTG、c-myc和p53在NSCLC中阳性表达率明显高于正常肺组织,阳性表达率差别有统计学意义(X~2=29.025,11.884,16.497;P=0.000,0.001,0.000)。
3.在NSCLC中PTTG、c-myc和p53的阳性表达率在不同年龄(X~2=0.019,0.221,0.729;P=0.991,0.895,0.694)、不同性别(X~2=0.057,0.286,0.011;P=0.812,0.593,0.916)和在不同病理类型中(X~2=0.302,0.020,0.350;P=0.582,0.888,0.554)的阳性表达率差别无统计学意义。
4.肿瘤直径D≤3cm组,NSCLC组织中PTTG、c-mvc和p53的阳性率分别为73.33%,53.33%和60%:3cm<D<5cm组中阳性率为71.43%,61.90%和47.62%:D≥5cm组阳性率为75.0%,58.33%和54.17%。经过统计学分析,在不同大小三组NSCLC中,PTTG、c-myc和p53的阳性表达率差别无统计学意义(X~2=0.073,0.264,0.550;P=0.964,0.876,0.760)。
5.PTTG、c-myc和p53在高分化NSCLC中的阳性表达率分别为35.71%,21.43%和57.14%,在中等分化者的阳性表达率为80.59%,71.43%和52.38%,在低分化者的阳性率为84.0%,68.0%和52.0%。PTTG、c-myc在三组不同分化程度NSCLC中的阳性表达率差别有统计学意义(X~2=8.727,10.288;P=0.013,0.006),p53在三组不同分化程度NSCLC中阳性表达率差别无统计学意义(X~2=0.107,P=0.948)。
6.淋巴结有转移组的NSCLC细胞PTTG、c-myc和p53阳性表达率分别为89.29%,75.0%和60.71%:淋巴结无转移组PTTG、c-myc和p53的阳性率分别为59.37%,39.29%和46.88%。PTTG、c-mvc在有转移组的NSCLC细胞中阳性表达率明显高于无转移组,阳性表达率差别有统计学意义(X~2=6.832,16.194,P=0.009,0.000)。p53在有转移和无转移的NSCLC细胞中阳性表达率差别无统计学意义(P=0.284)。
7.在NSCLC中PTTG和c-myc阳性表达分别为44例、35例,共同表达阳性为33例,共同表达阴性为14例,经Spearman等级相关分析NSCLC中PTTG阳性表达与c-myc呈显著正相关(rs=0.516,P<0.01);PTTG与p53阳性表达分别为44例、32例,共同表达阳性为29例,共同表达阴性为13例,经Spearman等级相关分析NSCLC中PTTG阳性表达与p53呈显著正相关(rs=0.418,P<0.01);c-myc与p53阳性表达分别为35例、32例,共同表达阳性为28例,共同表达阴性为11例,经Spearman等级相关分析c-myc阳性表达与p53呈显著正相关(rs=0.309,P<0.05)。
结论
1.PTTG,c-myc和p53在NSCLC组织中的阳性表达率均高于正常肺组织,提示三者均与NSCLC的发生有关。
2.PTTG与c-myc的表达与NSCLC细胞的分化程度、淋巴结转移关系密切,提示与NSCLC的发展有关。
3.PTTG、c-mvc在NSCLC中的表达呈显著正相关,提示两者在NSCLC发生和发展中存在协同作用;PTTG、p53在NSCLC中的表达呈显著正相关,提示两者在NSCLC发生和发展中存在协同作用;c-myc、p53在NSCLC中的表达也呈显著正相关,提示两者在NSCLC发生和发展中存在协同作用。
4.三者联合检测,有助于NSCLC评估预后和指导治疗。
Objective:
To investigate the relationship between the expression of PTTG;c-myc and p53 and their role in occurrence and development of non-small cell lung carcinoma.
Methods:
The expression of PTTG,c-myc and p53 was detected immunohistochemistrically by EnVision~(TM)method in 60 cases of non-small cell lung carcinoma and 27 cases of normal lung tissue.
Results:
1.There was an extensive expression of PTTG and c- myc in nucleus and cytoplasm of tumor cell(P<0.01),there was an extensive expression of p53 in nucleus of tumor cell(P<0.01).They were also existed less in normal lung tissue.
2.The positive rate of PTTG、c-myc and p53 was 73.33%,58.33% and 53.33%differencely in tumor cell and 11.11%,18.52%and 7.41% differencely in normal lung tissue.There was a significant difference between tumor cell and normal lung tissue.(X~2=29.025,11.884,16.497; P=0.000,0.001,0.000).
3.Difference was not found between the expression of PTTG and c-myc in age groups(X~2=0.019,0.221,0.729;P=0.991,0.895,0.694)and gender groups(X~2=0.057,0.286,0.011;P=0.812,0.593,0.916). Difference was also not found in groups of pathological tapes (X~2=0.302,0.020,0.350;P=0.582,0.888,0.554)and tumor size (X~2=0.073,0.264,0.550;P=0.964,0.876,0.760).
4.The expression of PTTG and c-myc in tumor cell was both correlated with differentiated degree of NSCLC(X~2=8.727,10.288;P= 0.013,0.006),the expression of p53 was not correlated with differentiated degree of NSCLC(X~2=0.107,P=0.948)
5.In NSCLC with lymph node metastasis,the positive rate of PTTG and c-myc was 89.29%,75.0%;and was 59.37%,39.29%respectively in groups without lymph node metastasis.There was a significant difference between the two groups(X~2=6.832,16.194,P=0.009,0.000).The positive rate of p53 was 60.71%,and was 46.88%in groups without lymph node metastasis.There was not a significant difference between the two groups(P=0.284).
6.The expression of PTTG and c-myc in non-small cell lung carcinoma had a striking positive correlation(rs=0.516,P<0.01);PTTG and p53 in non-small cell lung carcinoma had a striking positive correlation(rs=0.418,P<0.01);c-myc and p53 also had a striking positive correlation(rs=0.309,P<0.05).
Conclusion:
1.The positive rate of PTTG,c-myc and p53 was higher than that in normal lung tissue indicated that all were participated in occurrence of NSCLC.
2.Both PTTG and c-myc were correlated with differentiation of tumor cell and lymph node metastasis suggested they may involve in the development of NSCLC.
3.The positive expression of PTTG,c-myc and PTTG,p53 in non-small cell lung carcinoma had a striking positive correlation respectively(P<0.01),they were coordinated in occurrence and development of NSCLC.The positive expression of c-myc and p53 also had a striking positive correlation(P<0.05),and they were coordinated in occurrence and development of NSCLC too.
4.Detecting all of them would be beneficial to evaluating prognosis and the therapeutic guidance in NSCLC.
引文
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