鸡体内硫酸粘杆菌素残留检测微生物学方法和消除规律研究
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摘要
本实验建立了一种灵敏、快速和方便于实践应用的硫酸粘杆菌素残留检测微生物学方法。
该方法以支气管炎博代特氏菌(Bordetella bronchiseptica)ATCC4617作为检测菌株，应用一剂量法设计原理。用PBS提取肌肉组织和用5％硫酸提取肝脏和肾脏制备肌肉、肝脏和肾脏组织标准曲线，并以此标准曲线为基础计算待测样品中硫酸粘杆菌素的残留量。检测限肌肉组织为0.15μg／g、肝脏和肾脏组织均为0.20μg／g，低于我国农业部和欧盟规定的最高残留限量。在药物浓度为0.05μg／ml、0.2gg／ml和0.8μg／ml时日内变异系数分别为5.3％、3.6％和3.4％，日间变异系数分别为4.3％、2.4％和3.0％，说明本方法重复性好。肌肉、肝脏和肾脏组织标准曲线在0.025～6.4μg／ml范围内线性关系良好(r=0.9970，0.9994，0.9990：n=9，9，9)，肌肉在O.15μg／g、0.60μg／g及3.6μg／g添加水平下平均回收率分别为62.0％、79.0％和90.9％，变异系数为13.7％、11.4％和5.6％；肝脏在0.2μg／g、1μg／g及4μg／g添加水平下平均回收率分别为64.5％、80.2％和93.4％，变异系数为13.8％、5.1％和6.3％；肾脏在0.2μg／g、1μg／g及4μg／g添加水平下平均回收率分别为65.0％、81.8％和95.9％，变异系数为14.1％、7.2％和6.0％。
本实验首次研究了支气管炎博代特氏菌ATCC4617对多种抗菌药物的药敏实验，实验结果表明：该方法具有很高的特异性，但氯霉素和卡那霉素等药物有可能干扰检测结果的准确性或出现假阳性结果，可结合高效液相色谱等方法进行确证性实验。
以高、低(300mg／L、60mg／L按效价计)两个剂量给鸡饮水给药。肌肉高、低剂量组均未检测出硫酸粘杆菌素残留，肝脏和肾脏低剂量组未检测出硫酸粘杆菌素残留，高剂量组0h有微量残留。
This study was designed to establish a rapid, sensitive microbiological method in testing residues of CLS in various tissues of domestic animals.
The test organism used in this study was Bodetella Brochiseptica ATCC4617. Tissue samples of muscle, liver and kidney were collected and a standard regression curve for each tissue were made in accordance with the-dose theory. Computing quantity of CLS in samples based on the regression curve for the tissues. The detect limits of this test were 0.15 u g/g for the muscle tissue and 0.2 u g/g for the liver and kidney tissue that were lower than MRL of the EEC and the China Ministry of Agriculture .These regression curves for muscle, liver and kidney were satisfying linearity in the range of 0.025 u g/ml~6.4 u g/ml(r=0.9970,0.9994,0.9990; n=9,9,9). When CLS concentration was 0.05 u g/ml,0.2 u g/ml and 0.8 u g/ml respective, within-day coefficient of variation was 5.3%,3.6% and 3.4% respectively, between-day coefficient of variation was 4.3%, 2.4% and 3.0% respectively, the trial result indicate this method had good repetition. The mean recovery of muscle analysts at fortified level of 0.15 ug/g, 0.6 u g/g and 3.6 u g/g was 62.0%, 79.0% and 90.9% respectively, the coefficient of variation was 13.7%, 11.4% and 5.6% respectively ; the mean recovery of liver analysts at fortified level of 0.20 u g/g, 1 u g/g and 4 u g/g was 64.5%, 80.2% and 93.4% respectively, the coefficient of variation was 13.8%, 5.1% and 6.3% respectively ; The mean recovery of kidney analysts at fortified level of 0.20 u g/g, 1 u g/g and 4 u g/g was 65.0%, 81.8% and 95.9% respectively, the coefficient of variation was 14.1%, 7.2% and 6.0% respectively.
This study first tested the sensitivity of Bodetella Brochiseptica ATCC4617 to some drugs and the results showed that Bodetella Brochiseptica ATCC4617 had high specificity to CLS, but chloramphenicol and kanamycin maybe intefered with detecting result of the CLS and caused a false positive test, so the identification of CLS demand to link with some methods such as HPLC.
CLS was administered at dose levels of 60mg/L and 300mg/L in chicken in the drinking water. CLS activity was not detected in these samples for muscle tissue, could be detected trace amount of CLS in liver and kidney tissues in 300mg/L group at Oh.
该方法以支气管炎博代特氏菌(Bordetella bronchiseptica)ATCC4617作为检测菌株，应用一剂量法设计原理。用PBS提取肌肉组织和用5％硫酸提取肝脏和肾脏制备肌肉、肝脏和肾脏组织标准曲线，并以此标准曲线为基础计算待测样品中硫酸粘杆菌素的残留量。检测限肌肉组织为0.15μg／g、肝脏和肾脏组织均为0.20μg／g，低于我国农业部和欧盟规定的最高残留限量。在药物浓度为0.05μg／ml、0.2gg／ml和0.8μg／ml时日内变异系数分别为5.3％、3.6％和3.4％，日间变异系数分别为4.3％、2.4％和3.0％，说明本方法重复性好。肌肉、肝脏和肾脏组织标准曲线在0.025～6.4μg／ml范围内线性关系良好(r=0.9970，0.9994，0.9990：n=9，9，9)，肌肉在O.15μg／g、0.60μg／g及3.6μg／g添加水平下平均回收率分别为62.0％、79.0％和90.9％，变异系数为13.7％、11.4％和5.6％；肝脏在0.2μg／g、1μg／g及4μg／g添加水平下平均回收率分别为64.5％、80.2％和93.4％，变异系数为13.8％、5.1％和6.3％；肾脏在0.2μg／g、1μg／g及4μg／g添加水平下平均回收率分别为65.0％、81.8％和95.9％，变异系数为14.1％、7.2％和6.0％。
本实验首次研究了支气管炎博代特氏菌ATCC4617对多种抗菌药物的药敏实验，实验结果表明：该方法具有很高的特异性，但氯霉素和卡那霉素等药物有可能干扰检测结果的准确性或出现假阳性结果，可结合高效液相色谱等方法进行确证性实验。
以高、低(300mg／L、60mg／L按效价计)两个剂量给鸡饮水给药。肌肉高、低剂量组均未检测出硫酸粘杆菌素残留，肝脏和肾脏低剂量组未检测出硫酸粘杆菌素残留，高剂量组0h有微量残留。
This study was designed to establish a rapid, sensitive microbiological method in testing residues of CLS in various tissues of domestic animals.
The test organism used in this study was Bodetella Brochiseptica ATCC4617. Tissue samples of muscle, liver and kidney were collected and a standard regression curve for each tissue were made in accordance with the-dose theory. Computing quantity of CLS in samples based on the regression curve for the tissues. The detect limits of this test were 0.15 u g/g for the muscle tissue and 0.2 u g/g for the liver and kidney tissue that were lower than MRL of the EEC and the China Ministry of Agriculture .These regression curves for muscle, liver and kidney were satisfying linearity in the range of 0.025 u g/ml~6.4 u g/ml(r=0.9970,0.9994,0.9990; n=9,9,9). When CLS concentration was 0.05 u g/ml,0.2 u g/ml and 0.8 u g/ml respective, within-day coefficient of variation was 5.3%,3.6% and 3.4% respectively, between-day coefficient of variation was 4.3%, 2.4% and 3.0% respectively, the trial result indicate this method had good repetition. The mean recovery of muscle analysts at fortified level of 0.15 ug/g, 0.6 u g/g and 3.6 u g/g was 62.0%, 79.0% and 90.9% respectively, the coefficient of variation was 13.7%, 11.4% and 5.6% respectively ; the mean recovery of liver analysts at fortified level of 0.20 u g/g, 1 u g/g and 4 u g/g was 64.5%, 80.2% and 93.4% respectively, the coefficient of variation was 13.8%, 5.1% and 6.3% respectively ; The mean recovery of kidney analysts at fortified level of 0.20 u g/g, 1 u g/g and 4 u g/g was 65.0%, 81.8% and 95.9% respectively, the coefficient of variation was 14.1%, 7.2% and 6.0% respectively.
This study first tested the sensitivity of Bodetella Brochiseptica ATCC4617 to some drugs and the results showed that Bodetella Brochiseptica ATCC4617 had high specificity to CLS, but chloramphenicol and kanamycin maybe intefered with detecting result of the CLS and caused a false positive test, so the identification of CLS demand to link with some methods such as HPLC.
CLS was administered at dose levels of 60mg/L and 300mg/L in chicken in the drinking water. CLS activity was not detected in these samples for muscle tissue, could be detected trace amount of CLS in liver and kidney tissues in 300mg/L group at Oh.
引文
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