几种植物生长调节剂对马铃薯试管苗生长的影响
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摘要
试管苗作为马铃薯良种繁育的核心材料,其快繁壮苗是马铃薯种薯生产的基础环节。只有在有限时间内培育出根系发达、茎杆粗壮、有效茎节数多的试管苗,才更有利于新品种优质试管薯的诱导,对促进新品种在生产中尽快推广应用有着极其重要的现实意义。
本试验采用青海省农林科学院作物所马铃薯研究室育成的2个马铃薯品种,高淀粉型品种青薯2号和食品加工型品种青薯6号,研究了植物生长调节剂对马铃薯试管苗生长的影响,建立不同品种试管苗的优化培养体系,为马铃薯快繁提供理论依据,主要试验结论如下:
1.采用五因素(2,4-D、6-BA、NAA、KT、GA)四水平正交试验设计,研究了生长调节剂种类和浓度对两个品种试管苗株高、叶片数的影响。结果表明:不同生长调节剂和浓度对试管苗株高、叶片数有显著影响,说明不同品种的遗传基础不同,因此对于不同品种试管苗的培育应采用不同的培养基。
2.差异显著性检验表明:2,4-D对试管苗株高的作用最显著,5种激素对试管苗株高的影响顺序为:2,4-D>NAA >6-BA> GA> KT,适合株高生长的最佳培养基为MS基本培养基中附加2,4-D0.5 mg/L,6-BA1.0 mg/L,KT0.9 mg/L,GA6.5 mg/L;NAA对试管苗叶片生长的作用最显著,5种激素对试管苗叶片生长的影响顺序为:NAA > KT>6-BA> 2,4-D>GA,适合叶片生长的最佳培养基为MS基本培养基中附加NAA0.1mg/L,6-BA1.0 mg/L,KT0.1 mg/L,GA3.0 mg/L。
3.筛选出了两个品种试管苗培养基的最佳生长调节剂配方:适合青薯2号试管苗生长的最佳培养基为MS基本培养基添加2,4-D0.5 mg/L,6-BA1.0mg/L,KT0.9 mg/L,GA6.5 mg/L;适合青薯6号试管苗生长的最佳培养基为MS基本培养基添加2,4-D2.5mg/L,6-BA2.0mg/L,KT0.1 mg/L,GA10.0 mg/L。
4.运用饱和D最优设计方法,建立了不同植物生长调节剂、不同外植体数对试管苗生长的数学模型。最佳培养方案:试管苗最佳的外植体个数为7—9个,2,4-c最佳浓度为1.32mg/L—2.514mg/L;6-BA最佳浓度1.05mg/L—1.95mg/L;NAA最佳浓度0.165mg/L—0.335mg/L;KT最佳浓度0.35mg/L—0.65 mg/L;GA最佳浓度3.5mg/L—6.5mg/L.
As basic stock of potato breeding and multiplication, to obtain vigorous seedlings in vitro was a crucial step. How to obtain vigorous seedlings with health roots, thick stems and more functional nodes would be beneficial for inducing micro tubers. It was also a practical mean to boost popularization of new varieties.
Two varieties breed by laboratory of potato, institute of crop, agricultural academy of Qinghai province were applied to this test that are Qingshu 2 which was the high starch variety and Qingshu 6 which was the processing food varieties. In order to provide theoretical basis for rapid propagation, the effects of plant growth regulators on growth of in vitro potato platelet was studied. And the optimized culture system of different varieties of in vitro potato platelet was established. The major experimental conclusions were as follows:
1. The effects of kinds and concentration of plant growth regulators on plant height and leaf number of two varieties in vitro potato platelet was studied by orthogonal test design L16(45). The five-factor were 2,4-D,6-BA,NAA,KT and GA. The result showed that significant effect of different kinds and concentration of plant growth regulators on plant height and leaf number, which meant genetic background among varieties tested were different, and separate medium should be adopted for various varieties.
2. The test of the significance of difference was showed that 2,4-D had the most prominent effect on plant height followed by NAA, 6-BA, GA and KT. The best medium for lengthwise growth was MS with 2,4-D0.5 mg/L, 6-BA1.0 mg/L, KT0.9 mg/L and GA6.5 mg/L; NAA had the most prominent effect on leaf numbers followed by KT, 6-BA, 2,4-D and GA. The best medium for leaf growth was MS with NAA0.1mg/L, 6-BA1.0 mg/L, KT0.1 mg/L and GA3.0 mg/L.
3. It was screened out the optimum formula of plant growth regulators for culture medium of two varieties in vitro potato platelet. The best medium for growth of qingshu 2 was MS with 2,4-D0.5 mg/L, 6-BA1.0mg/L,KT0.9 mg/L and GA6.5 mg/L the growth; The best medium for growth of qingshu 6 was MS with 2,4-D2.5mg/L,6-BA2.0mg/L,KT0.1 mg/L and GA10.0 mg/L.
4. The mathematical model of different plant growth regulators and different explants on growth of in vitro potato platelet were established by D-saturating optimum design. Those were the optimum formula: the best explants number was 7—9; the best concentration of 2,4-D was 1.32mg/L—2.514mg/L; the best concentration of 6-BA was 1.05mg/L—1.95mg/L;the best concentration of NAA was 0.165mg/L—0.335mg/L; the best concentration of KT was 0.35mg/L—0.65mg/L,and the best concentration of GA was 3.5mg/L—6.5mg/L.
本试验采用青海省农林科学院作物所马铃薯研究室育成的2个马铃薯品种,高淀粉型品种青薯2号和食品加工型品种青薯6号,研究了植物生长调节剂对马铃薯试管苗生长的影响,建立不同品种试管苗的优化培养体系,为马铃薯快繁提供理论依据,主要试验结论如下:
1.采用五因素(2,4-D、6-BA、NAA、KT、GA)四水平正交试验设计,研究了生长调节剂种类和浓度对两个品种试管苗株高、叶片数的影响。结果表明:不同生长调节剂和浓度对试管苗株高、叶片数有显著影响,说明不同品种的遗传基础不同,因此对于不同品种试管苗的培育应采用不同的培养基。
2.差异显著性检验表明:2,4-D对试管苗株高的作用最显著,5种激素对试管苗株高的影响顺序为:2,4-D>NAA >6-BA> GA> KT,适合株高生长的最佳培养基为MS基本培养基中附加2,4-D0.5 mg/L,6-BA1.0 mg/L,KT0.9 mg/L,GA6.5 mg/L;NAA对试管苗叶片生长的作用最显著,5种激素对试管苗叶片生长的影响顺序为:NAA > KT>6-BA> 2,4-D>GA,适合叶片生长的最佳培养基为MS基本培养基中附加NAA0.1mg/L,6-BA1.0 mg/L,KT0.1 mg/L,GA3.0 mg/L。
3.筛选出了两个品种试管苗培养基的最佳生长调节剂配方:适合青薯2号试管苗生长的最佳培养基为MS基本培养基添加2,4-D0.5 mg/L,6-BA1.0mg/L,KT0.9 mg/L,GA6.5 mg/L;适合青薯6号试管苗生长的最佳培养基为MS基本培养基添加2,4-D2.5mg/L,6-BA2.0mg/L,KT0.1 mg/L,GA10.0 mg/L。
4.运用饱和D最优设计方法,建立了不同植物生长调节剂、不同外植体数对试管苗生长的数学模型。最佳培养方案:试管苗最佳的外植体个数为7—9个,2,4-c最佳浓度为1.32mg/L—2.514mg/L;6-BA最佳浓度1.05mg/L—1.95mg/L;NAA最佳浓度0.165mg/L—0.335mg/L;KT最佳浓度0.35mg/L—0.65 mg/L;GA最佳浓度3.5mg/L—6.5mg/L.
As basic stock of potato breeding and multiplication, to obtain vigorous seedlings in vitro was a crucial step. How to obtain vigorous seedlings with health roots, thick stems and more functional nodes would be beneficial for inducing micro tubers. It was also a practical mean to boost popularization of new varieties.
Two varieties breed by laboratory of potato, institute of crop, agricultural academy of Qinghai province were applied to this test that are Qingshu 2 which was the high starch variety and Qingshu 6 which was the processing food varieties. In order to provide theoretical basis for rapid propagation, the effects of plant growth regulators on growth of in vitro potato platelet was studied. And the optimized culture system of different varieties of in vitro potato platelet was established. The major experimental conclusions were as follows:
1. The effects of kinds and concentration of plant growth regulators on plant height and leaf number of two varieties in vitro potato platelet was studied by orthogonal test design L16(45). The five-factor were 2,4-D,6-BA,NAA,KT and GA. The result showed that significant effect of different kinds and concentration of plant growth regulators on plant height and leaf number, which meant genetic background among varieties tested were different, and separate medium should be adopted for various varieties.
2. The test of the significance of difference was showed that 2,4-D had the most prominent effect on plant height followed by NAA, 6-BA, GA and KT. The best medium for lengthwise growth was MS with 2,4-D0.5 mg/L, 6-BA1.0 mg/L, KT0.9 mg/L and GA6.5 mg/L; NAA had the most prominent effect on leaf numbers followed by KT, 6-BA, 2,4-D and GA. The best medium for leaf growth was MS with NAA0.1mg/L, 6-BA1.0 mg/L, KT0.1 mg/L and GA3.0 mg/L.
3. It was screened out the optimum formula of plant growth regulators for culture medium of two varieties in vitro potato platelet. The best medium for growth of qingshu 2 was MS with 2,4-D0.5 mg/L, 6-BA1.0mg/L,KT0.9 mg/L and GA6.5 mg/L the growth; The best medium for growth of qingshu 6 was MS with 2,4-D2.5mg/L,6-BA2.0mg/L,KT0.1 mg/L and GA10.0 mg/L.
4. The mathematical model of different plant growth regulators and different explants on growth of in vitro potato platelet were established by D-saturating optimum design. Those were the optimum formula: the best explants number was 7—9; the best concentration of 2,4-D was 1.32mg/L—2.514mg/L; the best concentration of 6-BA was 1.05mg/L—1.95mg/L;the best concentration of NAA was 0.165mg/L—0.335mg/L; the best concentration of KT was 0.35mg/L—0.65mg/L,and the best concentration of GA was 3.5mg/L—6.5mg/L.
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