决明子萌发过程中抗氧化活性变化规律研究
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摘要
决明子为豆科(Leguminosae)植物决明属(Cassia)的决明(Cassia obtusifolia L.)或小决明(Cassia tora L.)干燥成熟的种子,是常用的中药材。决明子含有蒽醌类、吡喃酮类、脂肪酸类、多糖类等化学成分。现代研究证明,决明子具有降压明目、调脂、保肝、抗氧化等作用,是保健食品、降脂减肥制剂和化妆品的原料。近年来,国内外对决明子的研究主要集中在决明子的化学成分、药理作用和临床应用、主要成分的提取分离以及含量测定等方面,同时对决明子的抗氧化活性也作了初步的研究。而关于决明子在萌发过程中抗氧化活性的动态变化规律还未见报道。
本文以决明(Cassia obtusifolia L.)干燥种子和不同萌发期决明子苗的干粉为材料,采用超声波提取法提取醇溶成分和水溶成分,通过醇溶成分和水溶成分总抗氧化活性、还原力、清除羟自由基活性、清除过氧化氢活性和清除DPPH自由基活性的测定,研究了决明子萌发过程中抗氧化活性的变化规律;采用苯酚—硫酸法测定萌发过程中决明子苗水溶性多糖的含量,分析水溶性多糖含量的变化与水提物抗氧化活性的变化间的相关性。研究结果对决明子的进一步合理开发和有效利用提供理论指导。论文取得如下主要结论:
(1)决明子种子本身和不同萌发期的决明子苗干粉醇提物和水提物均具有抗氧化活性。
(2)在决明子萌发过程中,醇提物的总抗氧化活性呈先下降后上升再下降的趋势,还原力、清除DPPH自由基活性、清除羟自由基活性均呈先上升后下降的趋势。在种子萌发后第7天,醇提物的抗氧化活性达到最强且均高于干燥种子。
(3)萌发后第7天的决明子苗醇提物的总抗氧化活性、还原力、清除羟自由基活性和清除过氧化氢活性明显弱于人工合成抗氧化剂BHT;清除DPPH自由基活性强于人工合成抗氧化剂BHT,清除DPPH自由基的IC_(50)(半抑制浓度)为0.80 mg/ml。
(4)在决明子萌发过程中,水提物的总抗氧化活性和清除DPPH自由基活性呈下降趋势,还原力呈先下降后上升再下降的趋势,清除羟自由基活性呈先上升后下降的趋势。
(5)在决明子萌发过程中,水提物多糖含量的变化与总抗氧化活性的变化、清除羟自由基活性的变化和清除过氧化氢的活性变化呈极显著正相关,与还原力变化呈负相关。
Cassia seed is the dry mature seed of Cassia obtusifolia L. or Cassia tora L. belonging to leguminosae Jue Ming genera (Cassia).. It is a common Chinese herbal medicine. It contains anthraquinones, pyranoid ketone, fatty acid, polysaccharides and so on. Modern research proved, Cassia seed has step-down and improving eyesight, adjusting lipid, protecting liver antioxidant effects and so forth. It is raw materials of health food, cosmetics, lipid-lowering and reducing weight drug. In recent years, the researches of Cassia seed at home and abroad are mainly concentrated in the chemical constituents,pharmacological functions, clinical applications, main component content of extraction and separation and so on, and at the same time the antioxidant activity also was made a preliminary study. Its antioxidant activity of dynamic change regulation in the process of germination has not been reported.
Dry powder of Cassia seeds and their seedlings at different germination stages as the material. Ethanol-soluble components and water soluble components were extracted by ultrasonic method. The changes of antioxidant activity in the process of germination were evaluated by total antioxidant activity, reducing power, removing·OH activity, removing H_2O_2 activity and DPPH·clearance activity. Phenol - sulfuric acid methods was used to determine the content of water-soluble polysaccharide of Cassia seed seedling in the process of germination, meanwhile correlation was analyzed between the changes in contents of water-soluble polysaccharide and the changes in antioxidant activity of water extracts. And with synthetic antioxidants BHT ( butylated hydroxytoluene ) as controls, the potential of extracts of Cassia seed seedling as raw material of natural antioxidant was evaluated at multi-angles, as Cassia seed rational development and efficient use of available theoretical basis. Main findings were as follows:
(1) Ethanol extracts and water extracts from dry powder of Cassia seeds and their seedlings at different germination stages all have antioxidant activity.
(2) In the process of germination, ethanol extracts of total antioxidant activity was decreased and then increased again before declining trend; reducing power, DPPH·clearance activity, removing·OH activity increased first and then decreased. On the 7th days after seed germination, ethanol extracts antioxidant activity was higher than dry seeds.
(3) Ethanol extracts on the 7th days after seed germination were weak in synthetic antioxidants BHT at Total antioxidant activity, reducing power, removing·OH activity and removing H_2O_2 activity. Its DPPH·clearance activity was stronger than BHT, and its IC_(50) (50% inhibiting concentration) of removing DPPH·was 0.80 mg/ml.
(4) In the process of germination, water extract of the total antioxidant activity and the DPPH·clearance activity decreased; reducing power was decreased and then increased again before declining trend, removing·OH activity was first increased and then decreased.
(5) In the process of germination, the changes of polysaccharide contents and the changes of total antioxidant activity, removing·OH activity, removing H_2O_2 activity and DPPH·clearance activity were obvious positively correlated. The changes of polysaccharide contents were negatively related to the changes of reducing power.
本文以决明(Cassia obtusifolia L.)干燥种子和不同萌发期决明子苗的干粉为材料,采用超声波提取法提取醇溶成分和水溶成分,通过醇溶成分和水溶成分总抗氧化活性、还原力、清除羟自由基活性、清除过氧化氢活性和清除DPPH自由基活性的测定,研究了决明子萌发过程中抗氧化活性的变化规律;采用苯酚—硫酸法测定萌发过程中决明子苗水溶性多糖的含量,分析水溶性多糖含量的变化与水提物抗氧化活性的变化间的相关性。研究结果对决明子的进一步合理开发和有效利用提供理论指导。论文取得如下主要结论:
(1)决明子种子本身和不同萌发期的决明子苗干粉醇提物和水提物均具有抗氧化活性。
(2)在决明子萌发过程中,醇提物的总抗氧化活性呈先下降后上升再下降的趋势,还原力、清除DPPH自由基活性、清除羟自由基活性均呈先上升后下降的趋势。在种子萌发后第7天,醇提物的抗氧化活性达到最强且均高于干燥种子。
(3)萌发后第7天的决明子苗醇提物的总抗氧化活性、还原力、清除羟自由基活性和清除过氧化氢活性明显弱于人工合成抗氧化剂BHT;清除DPPH自由基活性强于人工合成抗氧化剂BHT,清除DPPH自由基的IC_(50)(半抑制浓度)为0.80 mg/ml。
(4)在决明子萌发过程中,水提物的总抗氧化活性和清除DPPH自由基活性呈下降趋势,还原力呈先下降后上升再下降的趋势,清除羟自由基活性呈先上升后下降的趋势。
(5)在决明子萌发过程中,水提物多糖含量的变化与总抗氧化活性的变化、清除羟自由基活性的变化和清除过氧化氢的活性变化呈极显著正相关,与还原力变化呈负相关。
Cassia seed is the dry mature seed of Cassia obtusifolia L. or Cassia tora L. belonging to leguminosae Jue Ming genera (Cassia).. It is a common Chinese herbal medicine. It contains anthraquinones, pyranoid ketone, fatty acid, polysaccharides and so on. Modern research proved, Cassia seed has step-down and improving eyesight, adjusting lipid, protecting liver antioxidant effects and so forth. It is raw materials of health food, cosmetics, lipid-lowering and reducing weight drug. In recent years, the researches of Cassia seed at home and abroad are mainly concentrated in the chemical constituents,pharmacological functions, clinical applications, main component content of extraction and separation and so on, and at the same time the antioxidant activity also was made a preliminary study. Its antioxidant activity of dynamic change regulation in the process of germination has not been reported.
Dry powder of Cassia seeds and their seedlings at different germination stages as the material. Ethanol-soluble components and water soluble components were extracted by ultrasonic method. The changes of antioxidant activity in the process of germination were evaluated by total antioxidant activity, reducing power, removing·OH activity, removing H_2O_2 activity and DPPH·clearance activity. Phenol - sulfuric acid methods was used to determine the content of water-soluble polysaccharide of Cassia seed seedling in the process of germination, meanwhile correlation was analyzed between the changes in contents of water-soluble polysaccharide and the changes in antioxidant activity of water extracts. And with synthetic antioxidants BHT ( butylated hydroxytoluene ) as controls, the potential of extracts of Cassia seed seedling as raw material of natural antioxidant was evaluated at multi-angles, as Cassia seed rational development and efficient use of available theoretical basis. Main findings were as follows:
(1) Ethanol extracts and water extracts from dry powder of Cassia seeds and their seedlings at different germination stages all have antioxidant activity.
(2) In the process of germination, ethanol extracts of total antioxidant activity was decreased and then increased again before declining trend; reducing power, DPPH·clearance activity, removing·OH activity increased first and then decreased. On the 7th days after seed germination, ethanol extracts antioxidant activity was higher than dry seeds.
(3) Ethanol extracts on the 7th days after seed germination were weak in synthetic antioxidants BHT at Total antioxidant activity, reducing power, removing·OH activity and removing H_2O_2 activity. Its DPPH·clearance activity was stronger than BHT, and its IC_(50) (50% inhibiting concentration) of removing DPPH·was 0.80 mg/ml.
(4) In the process of germination, water extract of the total antioxidant activity and the DPPH·clearance activity decreased; reducing power was decreased and then increased again before declining trend, removing·OH activity was first increased and then decreased.
(5) In the process of germination, the changes of polysaccharide contents and the changes of total antioxidant activity, removing·OH activity, removing H_2O_2 activity and DPPH·clearance activity were obvious positively correlated. The changes of polysaccharide contents were negatively related to the changes of reducing power.
引文
陈燕芬,王燕玲,陈丽娟.2007.决明子的微波提取与含量测定.中国中药杂志,32(2):160-161
邓泽元,刘娟,余迎利,罗明祖.2002.决明子水溶性多糖提取的研究.食品科学,23(1):72-75
邓泽元,余迎利.2005.决明子中脂类的GC测定.食品科学,26(2):162-165
杜玉,娄红祥.2006.天然植物抗氧化剂的作用机制研究概况.中药材,29(7):739-743
樊青玲,张耀兮,李天才.2009.藜叶中黄酮类化合物体外抗氧化活性研究.天然产物研究与开发,21(4):672-675
高艳青,刘俊.2008.决明子的药理作用及其研究进展.中国药业,17(8):63-64
贾振宝,丁霄霖.2005.决明子提取物的体外抗氧化实验研究.食品与机械,22(6):44-45+54
郭威.2008.决明子鉴别及其主要有效成分含量测定的研究.[硕士学位论文].山东:山东大学
郭晓强,颜军,邬晓勇,徐光域,范春华,苟小军.2007.决明子水溶性多糖的纯化及抗氧化活性研究.食品科学,28(8):205-208
郭巧生.中国药用植物种子原色图鉴.2009.北京:中国农业出版社:139
韩立强,刘建华,杨国宇,王月影,朱河水,鲁维飞.2007.不同提取方法对决明子蒽醌类成分及抗氧化活性的影响.江苏农业科学,(6):255-258
何家庆,王兴,陈淑芳,常莉.2009.中药决明子萌发过程中生理生化变化的研究.中国中药杂志,34(8):1038-1040
侯长军,张平平,霍丹群.2007.决明子的应用研究进展.海峡药学,19(7):7-9
贾宝秀,齐永秀,张波,苗发陈.2008.流动注射化学发光法测定决明子中总蒽醌含量.泰山医学院学报,29(11):868-871
贾振宝,丁霄霖.2006.决明子中蒽醌化合物的提取工艺研究.食品与机械22(3):41-43
姜绍通,潘丽军,郑志.1998.速溶保健决明茶的研制.食品工业科技,(6):42-43
金鸣,蔡亚欣,李金荣,赵辉.1996.邻二氮菲-Fe2+氧化法检测H_2O_2/Fe2+产生的羟自由基.生物化学与生物物理进展,23(6):553-555
靳丹虹,梁芳慧,李敬筠,张寒琦.2007.微波辅助提取-HPLC测定决明子中的5种蒽醌类化合物.分子科学学报,23(6):429-433
李勇,孔令青,高洪,严玉霖.2008.自由基与疾病研究进展.动物医学进展,29(4):85-88
李磊,王岳飞,梁燕,杨贤强.2008.天然抗氧化物质的保健功能及抗氧化活性研究进展茶叶,34(2):70-74
李文,殷小杰,张村,李丽,王岚,肖永庆.2009.生、炒决明子水提物抗氧化及对一氧化氮、内皮素影响的比较研究.中国中药杂志,34(18):2383-2385
李玉美.2007.中药决明子的研究现状及综合利用.海峡药学,19(11):62-64
黎海彬,方昆阳,李续娥.2007.中药决明子蒽醌类成分含量测定的研究.食品科学, 28(7):427-429
黎海彬,方昆阳,吕翠婷.2007.决明子中蒽醌类成分超声波提取的研究.食品科学, 28(9):239-242
励娜,杨荣平,张小梅,龚桥,王宾豪.2007.决明子不同清炒品中蒽醌类成分的含量测定.海峡药学,19(8):42-43
刘斌,巩鸿霞,肖学凤,叶贵福.2010.决明子化学成分及药理作用研究进展.药物评价研究,33(4):312-315
刘安军,杨永久,张国蓉,曹东旭,王童.2006,生、炒决明子抗氧化作用的研究与比较.食品科技,(12):181-184
刘晓涵,陈永刚,林励,庄满贤,方晓娟.2009.蒽酮硫酸法与苯酚硫酸法测定枸杞子中多糖含量的比较.食品科技,34(9):270-272
刘娟,邓泽元,于化泓.2006.决明子水溶性多糖的抗氧化作用.食品科学,27(5):61-63
吕翠婷,黎海彬,李续娥,郭宝江.2006.中药决明子的研究进展.食品科技,(8):295-298
唐文婷,蒲传奋,陈实公.2008.决明子中蒽醌的乙醇浸提条件优化.食品工业,(1):16-18
王琴,蒋林,黄艳文,温其标.2005.超声波强化提取决明子活性成分.食品工业科术, 26(8):126-127+131
王琴,丁更强,黄艳文.2005.决明子山楂叶保健软糖的工艺研究.现代食品科技,21(2):91-93
王清华,纪玲,丛保忠,宋伟静.1996.高效液相色谱法测定决明子中大黄酚的含量.中医药学报,20(5):48-49
王会,郭立,谢文磊.2006.抗氧化剂抗氧化活性的测定方法(一).食品与发酵工业,32(3):92-98
王会,郭立,谢文磊.2006.抗氧化剂抗氧化活性的测定方法(二).食品与发酵工业, 32(4):98-102
吴银生.2002.决明子中蒽醌类成分的定量测定.江苏药学与临床研究,(1):24-25
谢亚.2007.中药决明子有效成分提取分离及结构的电喷雾串联质谱(ESI?MSn)研究.[硕士学位论文].天津:天津药学华南师范大学
肖培根.新编中药志.2001.北京:化学工业出版社:235
徐清萍,钟桂芳,孟君.2007.抗氧化剂抗氧化方法研究进展.食品工程,(2):23-25
晏马成,澄郭.2000.氧化损伤性疾病和中药的抗氧化治疗.时珍国医国药,11(8):765-766
杨黎燕.2003.决明子功效成分-蒽醌、多糖的提取及分析方法研究.[硕士学位论文].西安:西北大学
杨黎燕,郎惠云,王会玲,赵新法.2003.三氯化钛-分光光度法测定决明子蒽醌.中国油脂, 28(12):71-73
张名位,郭宝江.2001.果蔬抗氧化作用研究进展.华南师范大学学报(自然科学版).(4):115-121
张启伟,阴健,张俊.1996.生、炒决明子及其煎剂中活性成分比较.中草药,27(2):79-81
张自萍,黄文波,廖国玲,王玉炯.2007.枸杞子提取液抗氧化活性的研究.西北植物学报,27(5):859-863
张小梅,杨荣平,励娜,王宾豪,梁旭明.2007.决明子中蒽醌类成分的含量测定.时珍国医国药,18(1):97-98
张秋菊.1997.氧化损伤性疾病与抗氧化中药的研究.安徽中医学院学报,16(2):60-62
郑德勇,安鑫南.2004.植物抗氧化剂的研究概况与发展趋势.林产业化学与工业,24(3):113-118
郑德勇,安鑫南.2004.植物抗氧化剂研究展望.福建林学院学报,24(1):88-91
周小理,宋鑫莉,周一鸣,唐文.2009.苦荞萌发期抗氧化活性变化规律的研究.食品工业, (5):8-11
钟先锋,邓泽元,黄桂东.2004.决明子有效成分的提取.南昌大学学报(理科版),28(1):96-98
朱润芝,李京敬,谢超,郜尽,胡建军,袁运生,韩伟,俞雁.2010.过氧化作用与肝脏疾病.世界华人消化杂志,18(11):1134-1140
卓勇贤,郑仁兵,肖琳,李忠,唐浩国.2004.决明子酸豆奶的研制.食品科技,(11):64-66
曾军,石国荣.天然产物抗氧化活性的测定方法和原理.2008.安徽农学通报,22(14):35-36
左玉,谢文磊,王会.2006.生物抗氧化剂抗氧化作用的研究进展.食品与发酵工业,32(1):62-67
Asadi S, Ahmadiani A, Esmaeili MA, Sonboli A, Ansari N, Khodagholi F. 2010. In vitro antioxidant activities and an investigation of neuroprotection by six Salvia species from Iran: A comparative study. Food and Chemical Toxicology, 48(5): 1341-1349
Beevi S S, Mangamoori L N, Gowda B B. 2009. Polyphenolics and Antioxidant properties of Raphanus sativus L. Natural Product Research :1-30
Benzie I F F, Strain J J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1):70-76
Jia Z B, Tao F, Guo L, Jun T G, Ding X L. 2007. Antioxidant properties of extracts from juemingzi (Cassia tora L.) evaluated in vitro. Swiss Society of Food Science and Technology, 40:1072-1077
Kumaran A, Karunakaran R J. 2007. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. Lwt-Food Science and Technology, 40(2):344-352
Sui M W, Wong M M, Seligmann O, Wagner H. 1989. Anthraquinone glycosides from the seeds of Cassia tora. Phytochemistry, 28(1): 211-214
Wei Q, Ma X, Zhao Z, Zhang S, Liu S. 2010. Antioxidant activities and chemical profiles of pyroligneous acids from walnut shell. Journal of Analytical and Applied Pyrolysis, 88(2):149-154
Yen G C, Duh P D, Chuang D Y. 2000. Antioxidant activity of anthraquinones and anthrone. Food Chemistry, 70(4):437-441
邓泽元,刘娟,余迎利,罗明祖.2002.决明子水溶性多糖提取的研究.食品科学,23(1):72-75
邓泽元,余迎利.2005.决明子中脂类的GC测定.食品科学,26(2):162-165
杜玉,娄红祥.2006.天然植物抗氧化剂的作用机制研究概况.中药材,29(7):739-743
樊青玲,张耀兮,李天才.2009.藜叶中黄酮类化合物体外抗氧化活性研究.天然产物研究与开发,21(4):672-675
高艳青,刘俊.2008.决明子的药理作用及其研究进展.中国药业,17(8):63-64
贾振宝,丁霄霖.2005.决明子提取物的体外抗氧化实验研究.食品与机械,22(6):44-45+54
郭威.2008.决明子鉴别及其主要有效成分含量测定的研究.[硕士学位论文].山东:山东大学
郭晓强,颜军,邬晓勇,徐光域,范春华,苟小军.2007.决明子水溶性多糖的纯化及抗氧化活性研究.食品科学,28(8):205-208
郭巧生.中国药用植物种子原色图鉴.2009.北京:中国农业出版社:139
韩立强,刘建华,杨国宇,王月影,朱河水,鲁维飞.2007.不同提取方法对决明子蒽醌类成分及抗氧化活性的影响.江苏农业科学,(6):255-258
何家庆,王兴,陈淑芳,常莉.2009.中药决明子萌发过程中生理生化变化的研究.中国中药杂志,34(8):1038-1040
侯长军,张平平,霍丹群.2007.决明子的应用研究进展.海峡药学,19(7):7-9
贾宝秀,齐永秀,张波,苗发陈.2008.流动注射化学发光法测定决明子中总蒽醌含量.泰山医学院学报,29(11):868-871
贾振宝,丁霄霖.2006.决明子中蒽醌化合物的提取工艺研究.食品与机械22(3):41-43
姜绍通,潘丽军,郑志.1998.速溶保健决明茶的研制.食品工业科技,(6):42-43
金鸣,蔡亚欣,李金荣,赵辉.1996.邻二氮菲-Fe2+氧化法检测H_2O_2/Fe2+产生的羟自由基.生物化学与生物物理进展,23(6):553-555
靳丹虹,梁芳慧,李敬筠,张寒琦.2007.微波辅助提取-HPLC测定决明子中的5种蒽醌类化合物.分子科学学报,23(6):429-433
李勇,孔令青,高洪,严玉霖.2008.自由基与疾病研究进展.动物医学进展,29(4):85-88
李磊,王岳飞,梁燕,杨贤强.2008.天然抗氧化物质的保健功能及抗氧化活性研究进展茶叶,34(2):70-74
李文,殷小杰,张村,李丽,王岚,肖永庆.2009.生、炒决明子水提物抗氧化及对一氧化氮、内皮素影响的比较研究.中国中药杂志,34(18):2383-2385
李玉美.2007.中药决明子的研究现状及综合利用.海峡药学,19(11):62-64
黎海彬,方昆阳,李续娥.2007.中药决明子蒽醌类成分含量测定的研究.食品科学, 28(7):427-429
黎海彬,方昆阳,吕翠婷.2007.决明子中蒽醌类成分超声波提取的研究.食品科学, 28(9):239-242
励娜,杨荣平,张小梅,龚桥,王宾豪.2007.决明子不同清炒品中蒽醌类成分的含量测定.海峡药学,19(8):42-43
刘斌,巩鸿霞,肖学凤,叶贵福.2010.决明子化学成分及药理作用研究进展.药物评价研究,33(4):312-315
刘安军,杨永久,张国蓉,曹东旭,王童.2006,生、炒决明子抗氧化作用的研究与比较.食品科技,(12):181-184
刘晓涵,陈永刚,林励,庄满贤,方晓娟.2009.蒽酮硫酸法与苯酚硫酸法测定枸杞子中多糖含量的比较.食品科技,34(9):270-272
刘娟,邓泽元,于化泓.2006.决明子水溶性多糖的抗氧化作用.食品科学,27(5):61-63
吕翠婷,黎海彬,李续娥,郭宝江.2006.中药决明子的研究进展.食品科技,(8):295-298
唐文婷,蒲传奋,陈实公.2008.决明子中蒽醌的乙醇浸提条件优化.食品工业,(1):16-18
王琴,蒋林,黄艳文,温其标.2005.超声波强化提取决明子活性成分.食品工业科术, 26(8):126-127+131
王琴,丁更强,黄艳文.2005.决明子山楂叶保健软糖的工艺研究.现代食品科技,21(2):91-93
王清华,纪玲,丛保忠,宋伟静.1996.高效液相色谱法测定决明子中大黄酚的含量.中医药学报,20(5):48-49
王会,郭立,谢文磊.2006.抗氧化剂抗氧化活性的测定方法(一).食品与发酵工业,32(3):92-98
王会,郭立,谢文磊.2006.抗氧化剂抗氧化活性的测定方法(二).食品与发酵工业, 32(4):98-102
吴银生.2002.决明子中蒽醌类成分的定量测定.江苏药学与临床研究,(1):24-25
谢亚.2007.中药决明子有效成分提取分离及结构的电喷雾串联质谱(ESI?MSn)研究.[硕士学位论文].天津:天津药学华南师范大学
肖培根.新编中药志.2001.北京:化学工业出版社:235
徐清萍,钟桂芳,孟君.2007.抗氧化剂抗氧化方法研究进展.食品工程,(2):23-25
晏马成,澄郭.2000.氧化损伤性疾病和中药的抗氧化治疗.时珍国医国药,11(8):765-766
杨黎燕.2003.决明子功效成分-蒽醌、多糖的提取及分析方法研究.[硕士学位论文].西安:西北大学
杨黎燕,郎惠云,王会玲,赵新法.2003.三氯化钛-分光光度法测定决明子蒽醌.中国油脂, 28(12):71-73
张名位,郭宝江.2001.果蔬抗氧化作用研究进展.华南师范大学学报(自然科学版).(4):115-121
张启伟,阴健,张俊.1996.生、炒决明子及其煎剂中活性成分比较.中草药,27(2):79-81
张自萍,黄文波,廖国玲,王玉炯.2007.枸杞子提取液抗氧化活性的研究.西北植物学报,27(5):859-863
张小梅,杨荣平,励娜,王宾豪,梁旭明.2007.决明子中蒽醌类成分的含量测定.时珍国医国药,18(1):97-98
张秋菊.1997.氧化损伤性疾病与抗氧化中药的研究.安徽中医学院学报,16(2):60-62
郑德勇,安鑫南.2004.植物抗氧化剂的研究概况与发展趋势.林产业化学与工业,24(3):113-118
郑德勇,安鑫南.2004.植物抗氧化剂研究展望.福建林学院学报,24(1):88-91
周小理,宋鑫莉,周一鸣,唐文.2009.苦荞萌发期抗氧化活性变化规律的研究.食品工业, (5):8-11
钟先锋,邓泽元,黄桂东.2004.决明子有效成分的提取.南昌大学学报(理科版),28(1):96-98
朱润芝,李京敬,谢超,郜尽,胡建军,袁运生,韩伟,俞雁.2010.过氧化作用与肝脏疾病.世界华人消化杂志,18(11):1134-1140
卓勇贤,郑仁兵,肖琳,李忠,唐浩国.2004.决明子酸豆奶的研制.食品科技,(11):64-66
曾军,石国荣.天然产物抗氧化活性的测定方法和原理.2008.安徽农学通报,22(14):35-36
左玉,谢文磊,王会.2006.生物抗氧化剂抗氧化作用的研究进展.食品与发酵工业,32(1):62-67
Asadi S, Ahmadiani A, Esmaeili MA, Sonboli A, Ansari N, Khodagholi F. 2010. In vitro antioxidant activities and an investigation of neuroprotection by six Salvia species from Iran: A comparative study. Food and Chemical Toxicology, 48(5): 1341-1349
Beevi S S, Mangamoori L N, Gowda B B. 2009. Polyphenolics and Antioxidant properties of Raphanus sativus L. Natural Product Research :1-30
Benzie I F F, Strain J J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1):70-76
Jia Z B, Tao F, Guo L, Jun T G, Ding X L. 2007. Antioxidant properties of extracts from juemingzi (Cassia tora L.) evaluated in vitro. Swiss Society of Food Science and Technology, 40:1072-1077
Kumaran A, Karunakaran R J. 2007. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. Lwt-Food Science and Technology, 40(2):344-352
Sui M W, Wong M M, Seligmann O, Wagner H. 1989. Anthraquinone glycosides from the seeds of Cassia tora. Phytochemistry, 28(1): 211-214
Wei Q, Ma X, Zhao Z, Zhang S, Liu S. 2010. Antioxidant activities and chemical profiles of pyroligneous acids from walnut shell. Journal of Analytical and Applied Pyrolysis, 88(2):149-154
Yen G C, Duh P D, Chuang D Y. 2000. Antioxidant activity of anthraquinones and anthrone. Food Chemistry, 70(4):437-441