摘要
淫羊藿(Herba Epimedii)始载于《神农本草经》,味苦、辛,性温,归肝、肾经;具有补肾阳、强筋骨、祛风湿等功效,是药用历史悠久的传统中药。05版药典中收载了五个淫羊藿品种。其中,巫山淫羊藿与药典记载的其他品种相比容易鉴别:叶形狭长,长可达宽的6倍,叶中脉维管束为7个,叶片质地硬脆,现有大面积栽培基地,是淫羊藿目前药用的主流品种之一。
鉴于目前巫山淫羊藿药材运用广泛、疗效显著而其淫羊藿苷的含量远远达不到药典标准的情况下,有必要对目前研究较少的巫山淫羊藿化学成分进行研究,选择适合作为其质量控制指标的化学成分,并且期望以所分离得出的化合物单体作为物质基础,开展淫羊藿炮制品的相关研究。
此外,历代关于淫羊藿酒制品的记载有很多,十余部书籍及文献均有所提及淫羊藿酒制品,仅次于羊油制品,是古代淫羊藿药用的第二大炮制品,历代关于淫羊藿酒制品的运用比较广泛。但是以酒为辅料炮制,近代已经很少应用,目前已基本上被淘汰。酒制淫羊藿究竟有无道理,临床有无实效,应该经过研究证实之后再作肯定或否定的结论,必须持慎重态度,不要轻易抛弃前人的宝贵经验。因此,有必要对淫羊藿酒制品进行研究,再决定取舍。
同时,关于淫羊藿炮制的相关原理目前探讨较少,有必要进行深一步研究。
基于以上原因,我们主要从以下几个方面进行了研究:
1巫山淫羊藿化学成分的研究:采用溶剂法对巫山淫羊藿药材进行提取,使用多种色谱分离法和现代波谱分析方法进行化合物的分离和鉴定,从中分离得到11个化合物,鉴定了其中的8个,其中黄酮类有6个,分别为:朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、淫羊藿次苷Ⅰ、淫羊藿次苷Ⅱ,另外两个为β-谷甾醇、胡萝卜苷。其中朝藿定A为首次从巫山淫羊藿药材中分离得到。朝藿定C的含量在巫山淫羊藿药材中含量较高,甚至高于淫羊藿苷,并且在结构上淫羊藿苷多一个鼠李糖,二者结构十分相似,推测朝藿定C、淫羊藿苷在药效活性上可能有相似之处,可以考虑把朝藿定C作为评价巫山淫羊藿药材及饮片质量的指标之一。此外,首次对巫山淫羊藿叶中挥发油成份进行GC/MS分析。可知:巫山淫羊藿叶中的挥发油成分主要有酸类,酮类,烷类,醇类,炔类,其他化合物等组成。
2采用现代科学分析技术进行淫羊藿酒制品价值和意义的探索:首先结合药典规定的两个指标(淫羊藿苷、总黄酮)筛选出最佳酒溶媒为传统常用的绍兴黄酒,再参考古今文献中各种关于淫羊藿酒制品的炮制方法,结合现代相关炮制规范,用同一溶媒模拟炮制两个品种淫羊藿(巫山淫羊藿、淫羊藿)的多种酒制品:酒煮品、酒浸品、酒润品、酒蒸品、酒炙品、酒油共制品、同时炮制清炒品、目前运用广泛的羊脂油制品作为对照比较。然后,结合现代淫羊藿相关药理研究成果及实验中化学分离结果选用科学指标(朝藿定C、淫羊藿苷、总黄酮、多糖),对炮制后所得的各种炮制品饮片中指标成分的含量与生品饮片进行比较,进一步比较各炮制饮片与生品饮片水煎液中有效成分的溶出情况,综合分析可知:虽然淫羊藿品种不同所呈现出来的变化规律不同,但是酒制淫羊藿(酒蒸品、酒炙品、酒油共制品)与其他炮制品相比都显示出了优势,多个指标的评价均优于现行的油炙品。所得结果证明历代以淫羊藿酒制品用药确实有一定的科学道理,淫羊藿酒制品具有一定的意义和价值,现代抛弃淫羊藿酒制品的做法有待商榷,前人的宝贵经验应该继承下来,酒制淫羊藿具有恢复使用的意义。本课题初步筛选的几种酒制品(酒炙品、酒蒸品、酒油共制品)还有待深入探讨,最终期望确定一种酒制品,为淫羊藿临床运用增加一种炮制品,提高淫羊藿的药用价值。
3淫羊藿炮制品指纹图谱初探:利用HPLC梯度法初步建立了巫山淫羊藿及淫羊藿生品和多种炮制品黄酮类成分的指纹图谱。再对照巫山淫羊藿化学成分分析时得到的多个黄酮类单体,参考相关文献,指认出多个共有峰:朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、大叶淫羊藿苷F、箭藿苷B、次苷Ⅱ,占总面积的75%以上。比较发现:两个品种淫羊藿(巫山淫羊藿、淫羊藿)的指纹图谱有相似的地方-具有多个共同的峰,但是峰面积差别较大,其他多个非共有峰差别也较大,即不同品种之间黄酮类成分的组成和含量差异较大,所以应该针对不同品种淫羊藿建立相应的HPLC指纹图谱。此外,同法炮制来自同一产地同一品种,所得到的三个批次相同炮制品饮片的指纹图谱,通过相似度软件分析可知整体相似度在99%以上,说明同一产地的淫羊藿质量比较稳定,所用炮制工艺稳定性和重现性较好。此外,巫山淫羊藿、淫羊藿各炮制品与各自生品饮片相比,炮制前后多个共有峰的峰面积变化规律不完全相同,两种淫羊藿相同炮制品饮片间峰面积变化规律也不一。淫羊藿化学成分在炮制过程中的变化规律有待进一步深入研究。
对于多来源的中药,其内在成分的组成和含量存在明显差异,且炮制过程中化学成分的变化情况也不同,使得炮制所得饮片中及水煎液中主要成分的变化规律也有差别,指纹图谱峰数、共有峰峰面积等都有所不同,药典对不同品种、不同炮制饮片采用同一各质量标准似有不妥,建议药典针对不同品种、不同炮制饮片建立相应的质量标准,保证饮片质量的稳定、可控,提高中医临床的疗效。
As a group of traditional chinese herbs owning a long history and first recorded in Shennong's Canon of Herbal Materia Medica, Epimedium (Herba Epimedii) are characterized by bitter and acrid in taste and cold in nature, having significant effects on tonifying the liver and kidney、strengthening the bones and muscles、expelling wind and removing dampness. 5 varieties of Epimediums has been recorded in Chinese Pharmacopoeia (2005 Edition). By comparison to others, E.wushanense T.S.Ying is relatively easy to be distinguished: With hard and crispy texture of leaves, it has narrow-long blade profile, whose length is as long as 6 times than the width, and 7 vascular bundles in its midrib. Meanwhile, with broad cultivation planting bases, this one is used as the mainstream variety in clinical practice.
However E.wushanense T.S.Ying is widely in use and has significant medical effects, its content of Icariin is far below the Pharmacopoeia standard. Under such circumstances, it is of great necessity to study its chemical ingredients, which has not been appropriately studied yet. Through this research, it is expected to choose some proper chemical compositions as its quality control index and to isolated such compounds as its material foundation, on the basis of which to carry on studies on processed Epimedium.
In addition, famous rare books in past dynasties have many records about wine processed E.wushanense T.S.Ying which was mentioned in dozens of books and literatures used extensively as a medicine, only second to the one processed by mutton oil. But this class of E.wushanense T.S.Ying products was rarely used any longer and was replaced in principle. To avoid abandoning the valuable experience of our predecessors, it should be after considerate research on the wine processed E.wushanense T.S.Ying before making the conclusion whether it is reasonable to reject this wine processing technology and having clinical effects or not. On the other hand, because of the little research on the the processing mechanism of Epimedium, it is of great importance to do further study on it.
Therefore, based on the reasons mentioned above, this research was carried out from the following aspects:
1. studies on the chemical constituents of E.wushanense T.S.Ying: proper solvent method was applied in the extraction of E.wushanense T.S.Ying, whose chemical components was isolated and identified by integrating several chromatographic separation methods and modern spectral analysis methods, and 11 chemical compounds were obtained, among which 8 compounds was recognized. And 6 out of these 8 were flavonoids, which are Epimedin A isolated from E.wushanense T.S.Ying for the first time、Epimedin B、Epimedin C、Icariin、IcarisideⅠand IcarisideⅡ, besides the 6 mentioned above, the other 2 wereβ-sitosterol and daucosterol. The content of Epimedin C in E.wushanense T.S.Ying is quite high, even higher than that of Icariin, and with similar chemical structure, Epimedium C has just one more rhamnose than Icariin. Therefore, with so many similarities to each other, it is suggested that Epimedin C might have similar medical effects to Icariin, and from this point of view, the content of Epimedin C should be adopted as one of the quality indicating indexes. Meanwhile the essential oil in leaves of E.wushanense T.S.Ying was analyzed by GC/MS, and the result showed it consisted of acids、ketones、alkanes、alcohols、alkyne and other compounds.
2. The value and importance of wine processed E.wushanense T.S.Ying was explored by applying modern scientific analysis technology. Firstly, the yellow rice wine made in Shaoshing was selected as best menstruum by integrating the two indexes which were icariin and total flavonoids set by Chinese Pharmacopeia; secondly, by referring to the ancient and modern literatures and integrating the relevant modern processing specifications, several wine processed products of E.wushanense T.S.Ying and E.brevicornu Maxim. were produced with the yellow rice wine, such as: wine-boiled product、wine-soaked product、wine-moistened product、wine- steamed product、wine-stir-fried product、wine-mutton -oil processed product. At the same time, the simple stir-fried product and the mutton oil processed product were used as the comparison group. Then, scientific indexes, namely Epimedin C、Icariin、total flavorins and polysaccharide, were chosen by combination with relevant pharmacological results. After content determination of the selected indexes, comparison was made between the decoction pieces of crude epimedium and that of the processed epimedium, furthermore the dissolution cases was also under investigation between them. Through Multifactorial Analysis, conclusion has been made: although variation law of different species of Epimedium differed to each other, wine processed Epimedium (including wine- steamed product、wine-stir-fried product、wine-mutton -oil processed product ) had displayed many advantages over the rest, even better than the mutton -oil processed product which is broadly being use in clinic. All the results showed that wine processed Epimedium was reasonable and of great value for medical usage, which accounted for its popularity in past dynasties, so it remained to be discussed whether this process method should be discarded or not. The primarily selected wine processed products need deeper study till the best one is determined which will raise the medical valuation of Epimedium and add one more processed product to the clinical practice. 3. the fingerprints of the processed epimedium was primarily explored by following steps: firstly, the fingerprints of flavonoids in E.wushanense T.S.Ying、crude epimedium and several varieties of other processed products was primarily established through HPLC gradient methods. Then, most of the common peaks, such as:Epimedin A、Epimedin B、Epimedin C、Icariin、Ikarisoside F、SagittatosideB and IcariinⅡ, in the fingerprints was recognized which accounts for nearly more than 75% of the gross peak area. By comparison: the fingerprints of E.wushanense T.S.Ying and E.brevicornu Maxim. had lots of peaks in common, but there were big differences between the peak area of both the peaks in common and peaks that were not in common, which indicated that the content of the flavonoids in different species differed greatly and corresponding HPLC fingerprints should be established respectively. In addition, the similarity of the fingerprints of 3 different batches of same decoction pieces from the same habitat which were processed by identical method was above 99%. And the result showed that decoction pieces of epimedium from the same habitat had stable quality, and the processing technology had excellent stability and reproducibility. Meanwhile, by comparing to the decoction pieces of the crude herbs, the peak area variation law of the processec products of E.wushanense T.S.Ying and E.brevicornu Maxim.was not completely the same, which also varied between the two species processed by the same way. It is also expected that further study should be made to illuminate variation mechanism of the chemical compositions in epimedium during the processing .
we founded that there was obvious difference in the content and components-rate of change- discipline after precessed, and so was in water. So we advised that it shoud be established corresponding quality standard for several-origins Chinese Traditio and Medicine, in Chinese Pharmacopoeia (2005 edition) ,to ensure the quality stable and controllable, more to progress the clinical effect of Chinese Tradition and Medicine.
引文
[1] 徐国钧等主编.常用中药材品种整理和质量研究.南方协作组.第一册.福建科技,1994: 760-766
[2] 肖培根主编.新编中药志.第二册.化学工业出版社,2002:324-325
[3] 梁海锐,阎文玫.国产淫羊藿属叶表面特征的研究.西北植物学报,1992,12(2):142-148
[4] 李小洪等.陕西产淫羊藿根茎组织构造的研究及HPLC法测定淫羊藿苷的含量.西安医科大学学报,1997,18(4):479-480
[5] 梁海锐等.11种淫羊藿药材性状检索.中药材,1989,12(5):20-21
[6] 阎文玫等.淫羊藿的商品鉴定.北京中医药大学学报,1997,20(5):41-43
[7] 何顺志,郭宝林.贵州产淫羊藿属药用植物花粉形态的扫描电镜观察.中国中药杂志.1997,22(5):268-270
[8] 纪育.中国化学会志.1936,(4):312
[9] 桥本理作.实验动物学志.1987,14(2,3):195
[10] 刘宝庆.中草药.1980,(5):201
[11] 郭宝林,余竞光,肖培根.淫羊藿化学成分的研究.中国中药杂志,1996,21(5):290~292
[12] 崔东滨,徐雅娟等.朝鲜淫羊藿的化学成分研究.中国中药杂志,1993,18(11):683~684
[13] 李文魁,肖培根等.朝鲜淫羊藿的化学成分.天然产物研究与开发,1994,6(3):4~7
[14] 李文魁,肖培根等.朝鲜淫羊藿的化学成分(Ⅱ).天然产物研究与开发,1994,6(4):12
[15] 李文魁,张如意,肖培根.朝藿素B和朝藿素C的结构.药学学报,1994,29(11):835~839
[16] Li Wenkui et al.Chin Chem Lett,1994;5(11):945
[17] Li Wenkui et al.Phytochemistry,1995,38(3):807
[18] Li Wenkui et al.Phytochemistry.1995,38(1):263
[19] 李文魁等.高等学校化学学报.1995;16(2):230
[20] Yukio Ogihara et al.Chem Pharm Bμll.1995;43(4):703
[21] Li Wenkui et al.Phytoehemistry.1995;39(1):231
[22] 李文魁,张如意,肖培根.朝鲜淫羊藿化学成分的研究.中草药,1995,26(9):453~455
[23] 李文魁,肖培根等.朝鲜淫羊藿的化学成分(Ⅲ).中国药学杂志,1995,30(8):455~457
[24] Sun Pengyue et al.Chem Pharm Bμll.1995;44(2):446.
[25] 孙朋悦,陈英杰等.朝鲜淫羊藿化学成分研究.沈阳药科大学学报,1995,12(3):234
[26] Li Wenkui et al.Chin Chem Lett.1996;7(1):23
[27] 孙朋悦,陈英杰等.朝藿甙甲和朝藿甙乙的结构鉴定.药学学报,1996,31(8):602~606
[28] 李文魁,潘景歧,吕木坚等.朝藿甙A的结构.药学学报,1996,31(6):441~445
[29] 孙朋悦,徐颖等.朝鲜淫羊藿的化学成分Ⅱ.中国药物化学杂志,1998,8(4):281~284
[30] 孙朋悦,徐颖等.朝鲜淫羊藿的化学成分(Ⅰ).中国药物化学杂志,1998,8(2):122~125
[31] 孙朋悦,文晔,徐颖等.朝鲜淫羊藿的化学成分.药学学报,1998,33(12):911~919
[32] Li Wenkui et al.Phytochemistry.1996;43(2):527
[33] 李也实,刘永漋.柔毛淫羊藿化学成分研究.中草药,1992,23(1):8~11
[34] 桥本理作.实验动物学志 1987,14(2,3):195
[35] 刘宝庆.中草药,1980,(5):201
[36] Huang Yμlin et al.J Nat Prod.1993,56(2):275
[37] 吴勤丽,赵炎青,李珠莲.箭叶淫羊藿化学成分研究.中草药,1995,26(9):451~452
[38] Chen Chienchin et al.J Nat Prod.1996;59(4):412
[39] Huang Yulin et al.J Nat Prod.1993;56(2):275
[40] 徐凯建,顾风云等.淫羊藿叶中挥发油成分的气相色谱/质谱分析.中成药 1997,19(9):34
[41] 高子范,杨宗智,马克昌等.淫羊藿注射液对试管内鸡胚股骨生长的促进作用.中西医结合杂志,1985,5(3):172
[42] 李青南,吴铁等.淫羊藿提取液对去睾丸大鼠骨代谢的影响.中草药,1993,24(12):637
[43] 李青南,廖进民,吴铁等.淫羊藿提取液防治激素所致大鼠骨质疏松的实验研究.中国药学杂志,1996,31(8):467~470
[44] 马涛,崔燎,吴铁.中国骨质疏松杂志,2002,8(1):55~58
[45] 程庆,师锁柱,廖洪军.淫羊藿对 5/6 肾功能大鼠细胞免疫功能的影响.中国中西医结合杂志,1993,13(特):28
[46] 程庆,陈香美,师锁柱.淫羊藿对慢性肾衰大鼠免疫病理及细胞外基质的影响.中华内科杂志,1994,33(2):83
[47] 陈香美,廖洪军,李宁红等.慢性肾衰大鼠 IL—2 基因表达的改变及淫羊藿的调节作用.解放军医学杂志,1995,20(5):353~354
[48] 吴东方,马俊玲,周健等.淫羊藿提取液对庆大霉素急性肾损伤的影响.中国医院药学杂志,2002,22(5):270~271
[49] 孟宪丽,张艺,骆永珍等. 淫羊藿复合脂质体对正常及“阳虚”小鼠的 免疫调节作用.中国实验临床免疫学杂志,1995,7(4):38~40
[50] 刘佳,吴克枫.淫羊藿煎汁对小鼠免疫功能的影响.贵阳医学院学报,1996,21(1):17~18
[51] 张玲娟,沈自尹,蔡定芳.淫羊藿对大鼠下丘脑-垂体-肾上腺-胸腺轴抑制模型的影响.中医杂志,1996,37(10):620
[52] 刘佳,吴克枫等.淫羊藿对小鼠免疫功能的调节作用.卫生研究,1997,26(6):398~400
[53] 杨静玉,于庆海,李爽等.沈阳药科大学学报,1998,15(2):94
[54] 汪年松,唐令诠,竺艳娟,等.淫羊藿对血液透析 HCV 感染者细胞免疫功能的作用.上海免疫学杂志,2001,21(2):115~116
[55] 陈玮,邹焰,姜东.人参、淫羊藿增强小鼠免疫功能的协同作用.遵义医学院学报,2001,24(2):124~125
[56] 孙奕,王景明,骆永珍.淫羊藿对小鼠 MPS 吞噬功能和红细胞免疫粘附功能的影响及相关性研究.中国免疫学杂志,2002,18(2):114~116
[57] 曾靖等,淫羊藿水提取液局部麻醉作用的研究,赣南医学院学报,2004,24(1):1
[58] 李良东等,淫羊藿水提取液镇痛作用的研究,赣南医学院学报,2004,24(1):14
[59] 许青媛.淫羊藿对大鼠性腺功能的影响.中药药理与临床,1996,16(2):22
[60] 罗曼.淫羊藿对荷兰豚鼠发育的影响.中国野生植物资源,1993,17(1):38~39
[61] 曾南,孟宪丽,张艺.淫羊藿有效成分抗氧化作用研究.中国中药杂志,1997,22(1):46
[62] 李芳芳,李思,吕占军,等.淫羊藿苷对大鼠卵泡颗粒细胞和肾上腺皮质细胞分泌功能的影响.中国中药杂志,1997,22(8):499
[63] 边小丽,王苑桃等.淫羊藿多糖及水提液清除 O-2作用研究.西北药学杂,1999,14(2):79
[64] 王秋玲,林林等.中药淫羊藿诱导 HL-60 细胞程序死亡.天津医药 1997,25(11):652~653
[65] 曾南,孟宪丽,张艺.淫羊藿有效成分抗氧化作用研究.中国中药杂,1997,22(1):46
[66] 边小丽,王苑桃等.淫羊藿多糖及水提液清除 O-2作用研究.西北药学杂,1999,14(2):79
[67] 王秋玲,林林等.中药淫羊藿诱导 HL-60 细胞程序死亡.天津医药,1997,25(11):652~653
[68] 刘江波,邢善田.淫羊藿黄酮对抗小鼠 D-半乳糖衰老模型的研究.中药药理与临床,1990,6(2):18
[69] 高其铭,袁秉祥,赵苏玉等.淫羊藿总黄酮对家兔血小板功能及小鼠镇静、催眠及耐力作用的影响.西北药学杂志,1992,7(3):15
[70] 高其铭,袁秉祥,赵苏玉等.淫羊藿总黄酮对家兔血液流变学指标的影响.西安医科大学学报,1992,13(3):223
[71] 刘崇铭,王敏等.淫羊藿对脑血流与脑缺血的作用.沈阳药学院学报,1995,12(3):192
[72] 孟宪丽,张艺,李建亚等.淫羊藿总黄酮对大鼠卵泡颗粒细胞的影响.中国药理与临床,1998,14(4):10~11
[73] 许碧连,吴铁,崔燎等.淫羊藿总黄酮与雌激素合用对去卵巢大鼠骨质疏松的影响.中国药理学通报,2004,20(2):218~222
[74] 张逸凡,于庆海.淫羊藿总黄酮的抗炎作用.沈阳药科大学学报,1999,16(2):122
[75] 邹浩军,明亮.黄酮类化合物抗炎免疫及抗衰老药理研究进展.安徽卫生职业技术学院学报,2003,2(6):49
[76] 王敏,刘崇铭等.淫羊藿苷扩张脑血管作用的研究.沈阳药学院学报,1991,8(4):272
[77] 王敏,刘崇铭等.淫羊藿苷扩张脑血管作用的研究.沈阳药学院学报,1993,8(3):185
[78] 熊跃斌,周楚华.淫羊藿及菟丝子提取物对雄性生殖功能的影响.中国药学杂志,1994,29(2):89~91
[79] 李书桐,李铁军,郑钦岳等.淫羊藿苷对小鼠体外脾脏细胞增殖及产生集落刺激因子样活性的影响.第二军医大学学报,1995,16(4):340
[80] 崔正言,张玲,赵勇等.淫羊藿苷协同细胞因子及增强抗癌效应细胞活性的研究.中国肿瘤生物治疗杂志,1995,2(4):353
[81] 赵勇,崔正言,张玲等.淫羊藿苷协同诱生 IL-2、3、6 作用的研究.中国免疫学杂志,1996,12(1):43~45
[82] 赵勇,崔正言,张玲.淫羊藿甙对 HL-60 细胞诱导分化作用的研究,中华血液学杂志,1996,17(2):98
[83] 李晓冰,金东庆,郭良君等.淫羊藿甙在辐射小鼠免疫学功能恢复中作用的研究.中国辐射卫生,2002,11(3):171
[84] 林新,李文魁,罗崇念等.淫羊藿次苷β对三种肿瘤细胞株增殖的抑制作用.中国药理学与毒理学杂志,1997,11(3):183
[85] 丁雁,邢善田,周金黄.淫羊藿多糖促进小鼠 T 和 B 细 胞 3H-TdR 参入和诱生干扰素作用的研究.中国免疫学杂志,1985,1(6):42~46
[86] 王天然,邢善田,周金黄.淫羊藿多糖对初次、再次体液免疫应答的作用.中药药理与临床.1989,5(1):11~13
[87] 王天然,邢善田,周金黄.淫羊藿多糖和淫羊藿甙对抑制性 T 细胞的作用.中国免疫学杂志,1986,2(2):74~75
[88] 王鲁男,潘兴瑜,李淑华,等.淫羊藿多糖等四种药物对人 IL-2 活性影响的研究—— 小鼠胸腺细胞 MTT 比色分析法.锦州医学院学报,1990,11(2):39
[89] 王天然.淫羊藿多糖对小鼠巨噬细胞吞噬功能和淋巴细胞自发细胞毒作用的影响.中药药理与临床,1992,8(2):31
[90] 王鲁男,李淑华,潘兴瑜.淫羊藿多糖对人 IL-2 产生的调节作用.中国实验临床免疫学杂志,1993,5(3):41~44
[91] 丁雁.淫羊藿多糖致小鼠胸腺缩小的免疫药理机理研究.中国免疫学杂志 1993,9(3):185
[92] 丁雁等.淫羊藿和甙的胸腺免疫药理作用.中国药理学通报 1993,9(5):342~345
[93] 雷敩.雷公炮炙论.江苏科技出版社,1985:33
[94] 王衮.历代中医珍本集成(九)博济方.上海三联书店,1990:27
[95] 王怀隐.太平圣惠方.人民卫生出版社,1958:173
[96] 王怀隐.太平圣惠方.人民卫生出版社,1958:181
[97] 苏轼,沈括.苏沈良方.人民卫生出版社,1956:55
[98] 窦材.扁鹊心书.中医古籍出版社,1992:111
[99] 太医院.圣济总录.人民卫生出版社,1962:3022
[100] 赵佶.圣济总录.人民卫生出版社,1962:3018
[101] 唐慎微.重修政和经史证类备用本草.人民卫生出版社,1957:206
[102] 宋 太平惠民和剂局.太平惠民和剂局方.人民卫生出版社,1959:165
[103] 危亦林.世医得效方.上海科学技术出版社,1964:126
[104] 沙图穆苏.瑞竹堂经验方.上海卫生出版社,1957:8
[105] 朱震亨.丹溪心法.上海科技出版社,1959:204
[106] 朱橚.普济方.人民卫生出版社,1959:3306
[107] 龚廷贤.寿世保元.辽宁科学技术出版社,1997:145
[108] 陈嘉谟.本草蒙筌.人民卫生出版社,1988:196
[109] 张洁.仁术便览.人民卫生出版社,1985:307
[110] 张璐.本经逢源.上海科技出版社,1959:38
[111] 赵学敏.本草纲目拾遗.人民卫生出版社,1983:248
[112] 严西亭,施澹宁,洪缉庵.清·得配本草.中国中医药出版社,1979:36
[113] 林珮琴.类证治裁.上海中医药大学出版社,1997:384
[114] 屠道和.本草汇.上海科学技术出版社,2000:21
[115] 卫生部药典委员会.中华人民共和国药典 2005 版一部.人民卫生出版社,2004:229
[116] 卫生部药典委员会.中华人民共和国药典 2000 版一部.人民卫生出版社,2000:276
[117] 卫生部药典委员会.中华人民共和国药典 1995 版一部.人民卫生出版社,1995:287
[118] 卫生部药典委员会.中华人民共和国药典 1990 版一部.人民卫生出版社,1990:288
[119] 卫生部药典委员会.中华人民共和国药典 1985 版一部.人民卫生出版社,1985:288
[120] 卫生部药政管理局.全国中药炮制规范 1988 年版.人民卫生出版社,1988:245
[121] 牛锐,刘汇波.淫羊藿炮制前后对小鼠血浆睾酮及附性器官的影响.中国中药杂志,1989,14(9):18
[122] 崔学义,马英丽,孔琪,等.淫羊藿炮制工艺的研究.中国中药杂志,1996,21(7):408~409
[123] 徐世明,王远清,朱朝德.中药淫羊藿的质量研究-羊脂油炮制前后淫羊藿苷的含量比较.中草药,1985,(10):10
[124] 陈惠玲,王建科,张丽丽,等.粗毛淫羊藿及其不同炮制品中总黄酮的含量.中国中药杂志,2000,25(4):239~241
[125] 邹桂欣,姚燕.淫羊藿炮制前后多糖含量比较.中成药,1995,17(8):19
[126] 邹桂欣,翟宁.淫羊藿炮制前后锌锰铜含量比较.微量元素与健康研究,1996,13(1):35
[127] 陈惠玲,王建科,任永全,等.粗毛淫羊藿及其不同炮制品中 10 种微量元素的含量.中国中药杂志,2001,26(3):159~161
[128] 徐世明,王远清,朱朝德. 中药淫羊藿的质量研究-羊脂油炮制前后淫羊藿苷的含量比较. 中成药研究,1985(2):16~17
[129] 牛锐.淫羊藿炮制前后对小鼠血浆睾酮及附性器官影响.中国中药杂志,1989,14(9):18
[130] 崔学义,马英丽,孔琪等.淫羊藿炮制工艺的研究.中国中药杂志,1996,21(7):408
[131] 何伟.阳起石炮制前后 Cu、Mn、Zn 3 种元素含量的比较.全国第一届矿物药学术会议论文集.中国药学会,1989,8:143~146
[132] 卫生部药典委员会.中华人民共和国药典 2005 版一部.人民卫生出版社,2004:229
[133] 郭宝林,肖培根.四川产淫羊藿属植物的黄酮类成分分析.华西药学杂,2001,11(2):75
[134] 张艺,肖崇厚,孟宪丽等.8 种川产商品淫羊藿总黄酮的含量测定及资源利用.中国中药杂志,1995,20(4):201
[135] 施大文.淫羊藿质量考察分析.药学通报,1984,(11):57
[136] 徐世明,王远清,朱朝德. 中药淫羊藿的质量研究-羊脂油炮制前后淫羊藿苷的含量比较. 中草药,1985,(10):10
[137] 清·顾光重辑·神农本草经,人民卫生出版社.1955
[138] 宋·唐慎微:重修政和经史证类备用本草,人民卫生出版社,1957:206
[139] 明·卢之顾等:本草乘雅半偈,人民卫生出版社,1986:322
[140] 明·李时珍:本草纲目·人民卫生出版社,1957
[141] 清·黄宫绣:本草求真,上海科技出版社,1959:26
[142] 清·凌 鱼:本草害利,中医古籍出版社,1982:98
[143] 刘玉田.精子活力低下症的治疗方法.中医药信息,1987,(3):14
[144] 中国人民解放军 234 医院冠心病防治组.沈阳药学院学报,1977,(8):55
[145] 朱松年.生精育康胶囊的制备与临床应用.沈阳药学院学报,1975,(7):92
[146] 南京中医学院科研资料选编.1980,(1):43
[147] 浙江中医研究所.仙灵脾治疗高血压病 115 例的临床观察.浙江中医药,1977,3(6):11
[148] 张伯纳.中医治疗慢性乙型肝炎 36 例.中华内科杂志,1965,13(6):501
[149] 郭玉清.阳痿辨证新见.山东医学院学报,1978,(3):68
[150] 杜雨茂.心痛宁方治疗冠心病稳定型劳累性心绞痛 42 例.陕西中医,1987,(9):391
[151] 胡晓晨.肾病骨病的治疗经验.中医杂志,1988,(12):38
[152] 黄海波.“增精丸”治疗男性不育症 376 例临床观察.江西中医药,1989,(2):10
[153] 刘明汉.活血化瘀法治疗男性病举例.湖南医药杂志,1983,(1):9
[154] 龚朝晖.“四二五合方”治疗 36 例精子异常临床报告.江西中医药,1989:(6):14
[155] 高子范.慢性盆腔炎的辨证与治疗.中西医结合杂志,1985,5(3):172
[156] 陈树森.补肾活血法治疗排卵性障碍不孕经验.中国医药学报,1987:(11):21
[157] 王文键.中医药治疗慢性肾小球肾炎进展.中医杂志,1986,(4):32
[158] 张云如.补肾疏肝方治疗男性不育症 65 例.中医杂志,1988,(2):28
[159] 李海旺.淫羊藿制剂治疗神经衰弱 228 例疗效介绍.中药药理与临床,1982,23(11):70
[160] 陈仲雄.女性更年期综合症中医治疗进展.四川中医,1987,(11):21
[161] 郭建武.补肾疗法治疗妇女更年期综合症.陕西中医,1988,(2):21
[162] 钟达锦.中医药治疗室性早搏近况.中华血液学杂志,1988,(6):354
[163] 龚力.自拟调经汤治疗围绝经期宫血 56 例.中医杂志,1985,(1):65
[164] 恩施地区防治慢性气管炎办公室.湖北卫生,1972,(7):15
[165] 李昌.辨证治疗勃起障碍 54 例.浙江中医学院学报,1988,(3):35
[166] 广州市传染病院.新医药通讯(广州卫生局)1975,(6):12
[167] 郑州中药制药厂.中草药通讯,1972,(2):28
[168] 王寿卿.液化汤治疗精液不液化 55 例.中医杂志,1966,(4):30
[169] 重庆制药八厂.中草药通讯,1978,(6):15
[170] 刘伯岭.常见男性性功能障碍的诊治.新中医,1973(2):11
[171] 汪承柏.慢性肝炎辩证论治降酶的初步探讨.中西医结合杂志,1982,2(4):201
[172] 王永兴.试论黄酒的药用.浙江中医学院学报.1995,19(6):8
[173] 谢广发,戴军,陈尚卫等.科学认识黄酒的保健养生功能.中国酿造.2004,130(1):30
[1] 李文魁等. 万山淫羊藿的化学成分. 中国药学杂志,1996,31(6):332-334
[2] 李也实,刘永隆. 柔毛淫羊藿化学成分研究. 中草药,1992 ,23(1):8-11
[3] 邱峰等. 淫羊藿苷在大鼠体内的代谢. 药学学报, 1999,34(3):222-226
[4] 孙朋悦等. 东北淫羊藿活性成分的研究. 沈阳延科大学学报,1995,12(4):266-269
[5] 韩冰等. 黔岭淫羊藿化学成分的研究Ⅱ. 中国药学杂志,2002,37(10):740-741
[6] 李遇伯,孟繁浩,鹿秀梅,李发美,淫羊藿化学成分的研究,中国中药杂志,2005,30(8):586
[7] PU Cong-zhu (浦从珠) ,SU Ke-man (苏克曼) . Mass-Spect romet ry Fascicule of Analytical Chemistry Handbook ( Theninth fascicule ,the second edition) (分析化学手册.第九分册:质谱分册) [M] . Beijing (北京) :Chemical Indust ry Press(化学工业出版社) ,2000.
[8] 徐艳春,魏璐雪.华黄芪化学成分的研究.中国中药杂志,1995,20(5):296-297
[9] 国家药典委员会·中华人民共和国药典( 2005年版,一部)·北京:化学工业出版社, 2005,198
[1] 龚千峰 .中药炮制学 2003 年版.中国中医药出版社 2003:167
[2] 卫生部药典委员会.中华人民共和国药典 2005 版一部.人民卫生出版社,2005:229
[3] 卫生部药典委员会.中华人民共和国药典 2005 版一部.人民卫生出版社,2005:附录 27
[4] 龚千峰 .中药炮制学 2003 年版.中国中医药出版社 2003:90
[5] 龚千峰 .中药炮制学 2003 年版.中国中医药出版社 2003:290
[6] 龚千峰 .中药炮制学 2003 年版.中国中医药出版社 2003:307
[7] 朱永智、陈旭.不同品种、产地淫羊藿多糖的含量测定.天津中医药 2004,2(21):68
[8] 陈鸿英,朱永智,吴乃居,孙燕.朝鲜淫羊藿多糖的含量测定.中草药.2003,9:810
[9] 陈建芬、郭辉、马瑞彬.淫羊藿多糖的精制实验研究.时珍国医国药.2006:17(7):1241
[10] 孙玉书,毕力夫,苏秀兰.多糖及多糖类药物研究概况.内蒙古医学院学报.2006:28(1):75
[11] 梁惠花、刘晓河、王志宝、郭春燕.雷公藤多糖的提取和含量测定方法研究.中国现代应用药学杂志.2004,21(1):8
[1] 蔡宝昌,张志杰等.百合药材 GC 指纹图谱的建立.中华中医药学会第五届中药炮制学术会议论文集 2005
[2] 丁安伟,陈毓.小蓟及小蓟炭指纹图谱的初步研究.中华中医药学会第五届中药炮制学术会议论文集 2005
[3] 李丽,张村,刘元艳等.防风饮片 HPLC 指纹图谱的研究.中华中医药学会第五届中药炮制学术会议论文集 2005
[4] 张学兰,李慧芬,于雷.栀子药材及其饮片 HPLC 指纹图谱研究.中华中医药学会第五届中药炮制学术会议论文集 2005
[5] 杨云,闻永举,赵娟.酒黄芩 HPLC 指纹图谱研究.中华中医药学会第五届中药炮制学术会议论文集 2005
[6] 裴利宽.淫羊藿药 HPLC、IR 指纹图谱法鉴别和质量研究.中国协和医科大学论文.2006,5
[7] 易中宏,郑一敏,傅善全等.巫山淫羊藿不同部位的高校液相色谱指纹图谱测定.时珍国医国药,2005,16(5):377-378
[8] 黄朝瑜,赵陆华,梅玲华等.淫羊藿药材 HPLC 指纹图谱的研究,中国天然药,2003,9 (3):146-148