不同光照条件下NO_3~-胁迫对黄瓜幼苗生理生化特性的影响
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摘要
冬春季节日光温室蔬菜栽培条件下,低温、弱光及盐渍化构成影响蔬菜产量和品质的主要逆境因子,且往往是同时发生的。目前有关盐胁迫与光照影响植株生长的研究已有很多报道,但多是单因素研究,对盐胁迫与光照两个因子同时作用的研究报道很少,且盐胁迫多以NaCl处理为条件,而设施次生盐渍土的盐分组成特点和滨海、内陆盐土不同,其阴离子主要是NO_3~-,阳离子则以Ca~2+为主。本试验以我国北方日光温室主栽蔬菜作物黄瓜为研究对象,将硝酸盐胁迫与光照结合起来研究其对设施黄瓜幼苗生理生化特性的影响,为设施黄瓜生产提供理论指导。主要结果如下:
1 NO_3~-胁迫处理抑制了黄瓜幼苗的生长,增加了黄瓜幼苗光合色素的含量、降低了净光合速率(Pn)和气孔导度(Gs)。与高光照相比,低光照下幼苗生长抑制、净光合速率和气孔导度下降程度减轻,而光合色素的增加量升高。
2 NO_3~-胁迫处理使黄瓜幼苗保护酶SOD活性升高,叶片中POD、CAT、APX活性降低,而根中POD和APX活性升高,与高光照下的剧烈变化相比,低光照下的变化更平缓,低光照降低了NO_3~-胁迫处理与对照处理之间保护酶的活性差距。
3 NO_3~-胁迫处理使GS、GOGAT、GDH活性降低,与高光照相比,低光照下的降低幅度减小,低光照减轻了NO_3~-胁迫对氮代谢相关酶的负面效应。
Under the condition of solar glasshouse vegetable production in winter-spring season, low temperature, poor light and salt stresses constitute the main adverse circumstances factor of influencing vegetable yield and quality,and usually occur simultaneously. Currently there are many studies about the influences of salt stresses and light in plants,but most of them regarded single factor, there are fewer investigations about the two factor of salt stresses and light, and most of the research regarded NaCl as means of researching, but secondary salinization soil in the protected farmland is greatly different from seaside or inland, the main cation and anion are Ca~2+ and NO_3~-. In this research cucumber was selected as experimental material to study the physiological and biochemical of cucumber by the way of banding salt stresses and light together, in order to establish theory basis to cucumber production. The main results obtained were presented as follows:
1 NO_3~- stress inhibited cucumber seedlings growth, increased photosynthesis pigment content, decreased net photosynthetic rate (Pn) and stomatal conductance (Gs). Compare to high light intensity, low light intensity alleviate cucumber seedlings growth inhibition and the decrease of net photosynthetic rate and stomatal conductance and promoted the increment of photosynthesis pigment content
2 NO_3~- stress increased cucumber seedlings SOD, decreased POD、CAT、APX of leaves, but increased POD、APX of root, Compare to the acuity change of high light intensity, low light intensity was more calm, low light intensity reduce the difference between NO_3~- stress and comparison.
3 NO_3~- stress decreased cucumber seedlings GS、GOGAT、GDH, compare to high light, low light intensity alleviate the harm affection of NO_3~- stress to the nitrogen metabolism in cucumber seedlings.
1 NO_3~-胁迫处理抑制了黄瓜幼苗的生长,增加了黄瓜幼苗光合色素的含量、降低了净光合速率(Pn)和气孔导度(Gs)。与高光照相比,低光照下幼苗生长抑制、净光合速率和气孔导度下降程度减轻,而光合色素的增加量升高。
2 NO_3~-胁迫处理使黄瓜幼苗保护酶SOD活性升高,叶片中POD、CAT、APX活性降低,而根中POD和APX活性升高,与高光照下的剧烈变化相比,低光照下的变化更平缓,低光照降低了NO_3~-胁迫处理与对照处理之间保护酶的活性差距。
3 NO_3~-胁迫处理使GS、GOGAT、GDH活性降低,与高光照相比,低光照下的降低幅度减小,低光照减轻了NO_3~-胁迫对氮代谢相关酶的负面效应。
Under the condition of solar glasshouse vegetable production in winter-spring season, low temperature, poor light and salt stresses constitute the main adverse circumstances factor of influencing vegetable yield and quality,and usually occur simultaneously. Currently there are many studies about the influences of salt stresses and light in plants,but most of them regarded single factor, there are fewer investigations about the two factor of salt stresses and light, and most of the research regarded NaCl as means of researching, but secondary salinization soil in the protected farmland is greatly different from seaside or inland, the main cation and anion are Ca~2+ and NO_3~-. In this research cucumber was selected as experimental material to study the physiological and biochemical of cucumber by the way of banding salt stresses and light together, in order to establish theory basis to cucumber production. The main results obtained were presented as follows:
1 NO_3~- stress inhibited cucumber seedlings growth, increased photosynthesis pigment content, decreased net photosynthetic rate (Pn) and stomatal conductance (Gs). Compare to high light intensity, low light intensity alleviate cucumber seedlings growth inhibition and the decrease of net photosynthetic rate and stomatal conductance and promoted the increment of photosynthesis pigment content
2 NO_3~- stress increased cucumber seedlings SOD, decreased POD、CAT、APX of leaves, but increased POD、APX of root, Compare to the acuity change of high light intensity, low light intensity was more calm, low light intensity reduce the difference between NO_3~- stress and comparison.
3 NO_3~- stress decreased cucumber seedlings GS、GOGAT、GDH, compare to high light, low light intensity alleviate the harm affection of NO_3~- stress to the nitrogen metabolism in cucumber seedlings.
引文
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