短季棉群体的SOD活性研究
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摘要
棉花是我国的重要经济作物之一,也是关系到国计民生的重要的战略物资之一,它关系到我国数千万人的就业。但是我国人多地少,粮棉争地矛盾突出,解决这一矛盾的一个重要途径就是短季棉的推广。短季棉往往存在着早熟早衰的问题,其严重影响棉花的产量和质量。研究表明,超氧化物歧化酶(SOD)是在短季棉早熟不早衰复杂调控过程中一个很重要的酶类,是消除超氧阴离子的第一道防线。因此,在短季棉群体中对SOD进行研究具有重要意义,可以为短季棉的生化辅助育种提供理论依据。
本研究以早熟早衰中棉所10×早熟不早衰的辽4086为亲本建立的F2：6/F2：7群体为试验材料,分别从分子标记,蛋白,转录和田间性状的角度去研究SOD活性与早熟早衰和早熟不早衰的关系。开发了一个SOD活性的新STS标记,鉴定了以早熟早衰的中棉所10x早熟不早衰的辽4086为亲本建立的后代群体的早衰特性,研究了不同时期SOD同工酶的变化。主要研究结果如下：
1)、首先测定了本群体246个株系的SOD酶的活性,筛选出两组SOD酶活性差异较大的材料；提取了全部株系的DNA,然后用以SOD的EST序列设计的引物对两组SOD酶活性差异较大的材料的DNA进行扩增。在两组材料中,SOD酶活性大的材料扩增出587bp的片段,SOD酶活性小的扩增出842bp的片段；利用本群体对此标记验证表明,在全部的材料中,有56份扩增出587bp的片段,有190份扩增出842bp的片段,扩增出587bp片段材料的SOD酶活性的平均值约为840.476(unit/gFW)以上,但也有少量SOD活性低的材料扩增出短片段；扩增出842bp片段的SOD酶活性的平均值大约为690.276(unit/gFW),但也有少量SOD活性在840.476(unit/gFW)以上的材料扩增出长片段。说明此标记可用来判断短季棉SOD酶活性的高低。
2)、依据SOD酶活性变化的总规律,在2009年7月25日(Ⅰ),2009年8月10日(Ⅱ)及2009年8月25日(Ⅲ)分别对本群体的材料进行取样。测定酶活性,提取RNA,然后进行同工酶电泳和RT-PCR。结果表明,无论是在转录水平和翻译水平,SOD的表达量都在Ⅱ时期达到最高,说明SOD酶活性的变化趋势与它的基因表达量变化趋势是一致的。其中,Cu/Zn-SOD基因在构成总SOD酶活性中起着最大作用,有报道指出Cu/Zn-SOD活性可占到SOD总活性的86%。系统进化分析结果表明,在不同的植物中,棉花与葡萄的SOD基因相似性高,相同种类的SOD基因之间相似性高；在棉花中,这种趋势更为明显,且在相同组织中的各种SOD基因较为相似。
3)、对本群体田间调查的结果进行分析,结果表明：各个数量之间的交互作用关系是非常复杂的,既有相辅相成,也有互相制约,某一个性状的变化往往也会导致另外一些性状的变化。SOD与各性状(除整齐度外)都呈极显著的正相关,说明SOD对各性状所起的作用都为正效应。
Cotton is one of an import ecnomomic crops in china and it is strategic goods which have close relationship with our country's economy and people's life. It can influence tens millions of people's job in our country. However, our country has a large number of people while only has limited land, the contradictory between cotton and grains is very severe. An import way to solve this problem is promoting the short-seasoned cotton, but it always has the problem of early-decrepit which has negative influence on the yield and quality of cotton.SOD is an import enzyme in the complex process of short-seasoned cotton not early-decrepit and is the first line to get rid of ROS.So it is very important to carry some research on SOD, which can provide support to bio-chemical breeding.
This research is based on the population which is the descendant of ZhongmiansuolO and Liao4086, to probe the relationship between earliness with decrepit and earliness but not decrepit from the perspectives of molecular marker, protein, transcription and field characteristics.Develope a new SOD marker, detect the characteristics of this population and the change of SOD enzyme of different time. The main results are:
1)First determinated all of the 246 lines SOD activities and choose 2 groups:one is with high SOD activity and the other is with low SOD activity.Then uses the premiers which are desighed according to the EST of SOD to amplify their DNA. The results are:the group with high SOD activity have short fragment and the other group have long fragment. Then use all of the lines to check the reliability of this marker, we found that there are 56 lines are short fragments and 190 lines are long fragments. The average SOD activity of those lines which are 587bp is 840.476(unit/gFW)and the other is 690.276(unit/gFW). So this marker can be used to detect the SOD activity of short-seasoned cotton.
2) According to the regulation of the SOD activity in short-seasoned cotton, took the samples at July 25th 2009, August 10th 2009 and August 25th 2009. Determinated their activities, extracted RNA, and then used them to carry the further analysis.The results show that no matter at translation level or trascriptive level, the expression of SOD is highest atⅡ,the same as the change of SOD activity. Cu/Zn-SOD play a dominate role in the formation of the total SOD activity. Cotton SOD gene is very similar with grapes',and the sme type SOD is very same as each other and SODs in the same organ is very similar.
3) According to the analysis of field investigation results, the relationships among all the characteristics are complicated; they promote each other and limited each other, one's change often bring changes to others. SOD has predominated relationship with cotton field characteristics, this shows that SOD has positive influence on cotton field characteristics.
本研究以早熟早衰中棉所10×早熟不早衰的辽4086为亲本建立的F2：6/F2：7群体为试验材料,分别从分子标记,蛋白,转录和田间性状的角度去研究SOD活性与早熟早衰和早熟不早衰的关系。开发了一个SOD活性的新STS标记,鉴定了以早熟早衰的中棉所10x早熟不早衰的辽4086为亲本建立的后代群体的早衰特性,研究了不同时期SOD同工酶的变化。主要研究结果如下：
1)、首先测定了本群体246个株系的SOD酶的活性,筛选出两组SOD酶活性差异较大的材料；提取了全部株系的DNA,然后用以SOD的EST序列设计的引物对两组SOD酶活性差异较大的材料的DNA进行扩增。在两组材料中,SOD酶活性大的材料扩增出587bp的片段,SOD酶活性小的扩增出842bp的片段；利用本群体对此标记验证表明,在全部的材料中,有56份扩增出587bp的片段,有190份扩增出842bp的片段,扩增出587bp片段材料的SOD酶活性的平均值约为840.476(unit/gFW)以上,但也有少量SOD活性低的材料扩增出短片段；扩增出842bp片段的SOD酶活性的平均值大约为690.276(unit/gFW),但也有少量SOD活性在840.476(unit/gFW)以上的材料扩增出长片段。说明此标记可用来判断短季棉SOD酶活性的高低。
2)、依据SOD酶活性变化的总规律,在2009年7月25日(Ⅰ),2009年8月10日(Ⅱ)及2009年8月25日(Ⅲ)分别对本群体的材料进行取样。测定酶活性,提取RNA,然后进行同工酶电泳和RT-PCR。结果表明,无论是在转录水平和翻译水平,SOD的表达量都在Ⅱ时期达到最高,说明SOD酶活性的变化趋势与它的基因表达量变化趋势是一致的。其中,Cu/Zn-SOD基因在构成总SOD酶活性中起着最大作用,有报道指出Cu/Zn-SOD活性可占到SOD总活性的86%。系统进化分析结果表明,在不同的植物中,棉花与葡萄的SOD基因相似性高,相同种类的SOD基因之间相似性高；在棉花中,这种趋势更为明显,且在相同组织中的各种SOD基因较为相似。
3)、对本群体田间调查的结果进行分析,结果表明：各个数量之间的交互作用关系是非常复杂的,既有相辅相成,也有互相制约,某一个性状的变化往往也会导致另外一些性状的变化。SOD与各性状(除整齐度外)都呈极显著的正相关,说明SOD对各性状所起的作用都为正效应。
Cotton is one of an import ecnomomic crops in china and it is strategic goods which have close relationship with our country's economy and people's life. It can influence tens millions of people's job in our country. However, our country has a large number of people while only has limited land, the contradictory between cotton and grains is very severe. An import way to solve this problem is promoting the short-seasoned cotton, but it always has the problem of early-decrepit which has negative influence on the yield and quality of cotton.SOD is an import enzyme in the complex process of short-seasoned cotton not early-decrepit and is the first line to get rid of ROS.So it is very important to carry some research on SOD, which can provide support to bio-chemical breeding.
This research is based on the population which is the descendant of ZhongmiansuolO and Liao4086, to probe the relationship between earliness with decrepit and earliness but not decrepit from the perspectives of molecular marker, protein, transcription and field characteristics.Develope a new SOD marker, detect the characteristics of this population and the change of SOD enzyme of different time. The main results are:
1)First determinated all of the 246 lines SOD activities and choose 2 groups:one is with high SOD activity and the other is with low SOD activity.Then uses the premiers which are desighed according to the EST of SOD to amplify their DNA. The results are:the group with high SOD activity have short fragment and the other group have long fragment. Then use all of the lines to check the reliability of this marker, we found that there are 56 lines are short fragments and 190 lines are long fragments. The average SOD activity of those lines which are 587bp is 840.476(unit/gFW)and the other is 690.276(unit/gFW). So this marker can be used to detect the SOD activity of short-seasoned cotton.
2) According to the regulation of the SOD activity in short-seasoned cotton, took the samples at July 25th 2009, August 10th 2009 and August 25th 2009. Determinated their activities, extracted RNA, and then used them to carry the further analysis.The results show that no matter at translation level or trascriptive level, the expression of SOD is highest atⅡ,the same as the change of SOD activity. Cu/Zn-SOD play a dominate role in the formation of the total SOD activity. Cotton SOD gene is very similar with grapes',and the sme type SOD is very same as each other and SODs in the same organ is very similar.
3) According to the analysis of field investigation results, the relationships among all the characteristics are complicated; they promote each other and limited each other, one's change often bring changes to others. SOD has predominated relationship with cotton field characteristics, this shows that SOD has positive influence on cotton field characteristics.
引文
[1]陈新华,郭宝林,赵静,杨俊霞.休眠期内甜樱桃不同品种枝条的抗寒性[J].河北农业大学学报,2009,6(32)：37-40
[2]程光宇,王峰,陶明煊,陆长梅,吴国荣,常福辰.聚丙烯酰胺梯度凝胶电泳和均一凝胶电泳在分离及鉴定植物SOD同工酶中的比较研究[J].南京师大学报(自然科学版),2008,1001-4616(2008)04-0097-07
[3]崔瑞峰,杜娟.甘蓝幼苗感染黑腐病后SOD、POD活性的变化[J].2009,34(5)：35—37,40
[4]陈锦云,林祥永,兰志斌等.超氧化物歧化酶防治烟草气候斑点病效果初报.福建农业科技,2006,(5)：13
[5]陈忠宁,毛宗万,唐雯霞.铜锌超氧化物歧化酶和结构、机理及其模拟研究进展.化学通报,1999,6：1-8
[6]程光宇,魏锦城,邹玉珍,吴国荣.拘祀果实Cu/Zn-SOD的纯化及其性质的研究.南京师范大学学报(自然科学版),1991,14(2)：82-92
[7]崔京日,黄世臣.水分胁迫及接种VAM对山杏叶片SOD活性和MDA含量变化的影响[J].延边大学农学学报,2009,31(2)：84-88
[8]戴鑫,张边江,章琦,毛善国.盐胁迫下不同浓度的锰处理对小麦幼苗生长及SOD.POD的影响[J].安徽农业科学.2009,37(27)：13000-13001
[9]耿军义,张香云,崔瑞敏等.杂交棉冀棉18号一代、二代的SOD和POD活性及生理生化机制研究[J].华北农学报,2002,17(4)：96-99
[10]高学曾,许金芳等.玉米正常粒和败育粒淀粉磷酸化酶、过氧化酶活必及其同工短季棉早熟不早衰生化机制及遗传研究酶的比较[J].植物生理学通讯,2007,(4)：30-35
[11]高俊杰,秦爱国,于贤昌.低温胁迫下嫁接对黄瓜叶片SOD和CAT基因表达与活性变化的影响.应用生态学报,2009,20(1)：213-217
[12]葛知男.陆地棉早熟性的生理特点及其遗传研究[J].江苏农业科学,1993,(1)：21-24
[13]郭海军,董志强,林永增等.黄萎病对棉花叶片SOD、POD酶活性和光合特性的影响[J].中国农业科学,2006,25(6)：40-46
[14]贺鹏,朱列书,钟波.航天诱变对烤烟大田期SOD活性的影响[J].中国农学通报,2008,24(5)：123-125。
[15]黄绍兴,阎隆飞.植物发育过程中特异基因的表达[J].植物生理学通讯,1995,31(30)：161-166
[16]李大辉.铅污染对菱幼苗SOD、POD及叶细胞亚显微结构的影响。中国环境科学,2009,29(2)：136-141
[17]李筠,邓西平,郭尚洙,田中净.转铜/锌超氧化物歧化酶和抗坏血酸过氧化物酶基因甘薯的耐旱性,植物生理与分子生物学学报,2006,32(4)：451-457
[18]龙书生,曹远林,李亚玲,康振生.小麦抗条锈病过敏性坏死反应中的活性氧代谢[J].西北农林 科技大学学报(自然科学版),2009,11：125-130
[19]林庆斌,丁杨,王海波,张昭其,毛小云,乐学义.盐胁迫下SOD模拟物对水稻幼苗生物量及SOD活性的影响[J].土壤通报.2009,5：1163-1166
[20]罗广华,王爱国.植物SOD的凝胶电泳及活性显示[J].植物生理学通讯,1983,(6)：44-45.
[21]罗广华,王爱国.植物SOD同工酶活性显示的某些干扰[J].植物生理学通讯,1993,29(2)：119-122.
[22]吉文成,周有耀.棉花早熟性与纤维性状的遗传分析[J].北京农业大学学报,1994,20(4)：389-396
[23]蒋选利,李振岐,康振生.过氧化物酶与植物抗病性研究进展.西北农林科技大学学报(自然科学版),2001,29(6)：124-129
[24]姜瑞云,王宏,黄祯茂等.浅谈棉花早衰[J].中国棉花,1996,23(3)：7-8
[25]刘凤权,王金生.水杨酸对水稻叶片抗氧化酶系的影响[J].农业生物技术学报,2001,9(4)：396-399
[26]马淑娟,喻树迅,范术丽,宋美珍.转棉花叶绿体Cu/Zn-SOD基因烟草的获得及其功能的初步验证分子植物育种,2007,Vol.5,No.3,319-323
[27]潘家驹,阂留芳,吴振衡二陆地棉若干性状之间的遗传相关及遗传力的初步研究[J].江苏农业科学,1979,(4)：1-7
[28]潘家驹.作物育种学总论[M].中国农业出版社,1998
[29]潘家驹.棉花育种学[M].中国农业出版社,1995,p.273-295
[30]潘哲超,陈建斌,范静华,周惠萍,邱世刚,叶漪,果志华.稻瘟菌粗毒素对水稻防御性相关酶系的诱导.云南农业大学学报,2008,1004-390X(2008)02-0162-05
[31]秦水琼,贾士荣.植物抗氧化逆境的基因下程.农业生物技术学报,1997,5(1)：15-22
[32]盛良全,郑晓云,门向阳,肖厚荣,厦炳乐,刘少民.生命体中的超氧化物歧化酶.医学基础与药学研究,2002,48(6)：48-53
[33]石春海,吴建国,樊龙江,朱军,吴平.不同环境条件下稻米透明度的发育遗传研究分析[J].遗传学报,2002,29(1)：56-61
[34]汤学军,王康.激动素和抗坏血酸保护受冷害苗细胞膜和促进SOD合成的效应[J].植物学报,1993,(增刊)：45-49
[35]唐薇,李维江,张冬梅等.干旱对转基因抗虫棉苗期叶片POD、MDA和光合速率的影响[J].中国棉花,2002,29(2)：23-24
[36]陶均,李玲.高等植物脱落酸生物合成的酶调控.植物学通报,2002,19(6)：675-683
[37]田志刚,张淑芳,丁智明.短季棉株型性状与经济性状关系初探[J].中国棉花,1999,26(2)：18-19
[38]田志刚,郝笑琦,张淑芳.短季棉早熟性状及产量构成因素的遗传分析和选择策略[J],华北农学报,1993,8(1)：18-22
[39]田蓄华.早熟陆地棉主要性状的遗传率及遗传进度的研究[J].遗传,1983,5(1)：15～16
[40]万永吉,郑文教,方煜,王铮敏,邱瑾.重金属铬(Ⅲ)胁迫对红树植物秋茄幼苗SOD、POD活性 及其同工酶的影响,厦门大学学报,2008,0438-0479(2008)04-0571-04)
[41]魏玉香,李永红,谷茂,刘晓辉.鸡冠花幼苗热胁迫耐性与其SOD之间的关联.西北植物学报,2008,28(10)：2055-2061
[42]王兆龙,曹卫星.细胞分裂素对植物基因表达的调节[J].植物生理学通讯,2000,36(1)：82-88
[43]王国山,顾恒兰,毕淑兰.短季棉主要性状遗传分析与改良[J].沈阳农业大学学报,1998,29(1)：6-1
[44]王国印.陆地棉数量性状遗传研究与进展[J].中国棉花,1994,21(3)：5-7
[45]王建华,刘鸿先,徐同等.超氧化物岐化酶SOD在植物逆境和衰老生理中的作用[J].植物生理学通讯,1959,25(1)：1-7
[46]王忠军,陈爱民,王沛政等.陆地棉主要经济性状的配合力和遗传力分析[J].新疆农业大学学报,1999,22(一)：69-72
[47]王莹,戴继勋.裙带菜同工酶酶谱特征及其遗传多样性的研究.遗传学报,1999,26(3)269～273
[48]王雅平,刘伊强,施磊等.小麦对赤霉病抗性不同品种的50D活性[J].植物生短季棉早熟不早衰生化机制及遗传研究理学报,1993,19(4)：353-358
[49]王淼,李秋荣,付士磊,董百丽.外源一氧化氮对干旱胁迫下杨树光合作用的影响,应用生态学报2005,16(2)：218～222
[50]王璞,陈咖.植物激素脱落酸的信号转导[J].植物生理学通讯,2000,36(2)：165-169
[51]温伟庆,陈友吾.银杏雌雄株过氧化物酶和过氧化氢酶活性差异研究.福建林业科技,2002,29(2)：34-39
[52]许长成,邹琦.大豆叶片旱促衰老及其与膜脂过氧化的关系[J].作物学报,1993,19(4)：360-364
[53]许长成,赵世杰,邹奇.植物组织内丙二醛的分离与鉴定[J1.植物生理学通讯,1992,28(4)：189-198
[54]汤章城.植物对水分胁迫的反应和适应性n.植物对干旱的反应和适应性.植物生理学通讯,1983,(4)：1-7
[55]王关林,方宏绮.植物基因工程(第二版).北京：科学出版社,2002：527-528
[56]文才艺,吴元华,李浩戈等.接种PVYN后烟草叶片SOD活性和MDA含量的变化.中国烟草科学,1999,(1)：12-14
[57]徐德利,宋平.大麦感染黄花叶病后POD、SOD活性变化规律的初步研究[J]。南京师大学报(自然科学版),2008,31(2)：101-104
[58]孙天国,沙伟.盐胁迫对甜瓜种子萌发及幼苗SOD.POD活性的影响[J].安徽农业科学,2009,37(24)：11509-11510,11513
[59]闰新甫.全球转基因作物种植概况.世界农业,2001,4：22-3
[60]杨答,邹国林,朱汝番.鸭血超氧化物歧化酶的纯化及性质研究.生物化学杂志.1989,5(2)：169
[61]姚广,高辉远,王未未,张立涛,部建雯,铅胁迫对玉米幼苗叶片光系统功能及光合作用的影响.生态学报,2009,29(3)：1162～1169.
[62]叶子弘,朱军.陆地棉开花成铃性状的遗传研究-不同发育阶段的遗传规律[J].遗传学报,2000,27(9)：800-809
[63]叶子弘,朱军.陆地棉开花成铃性状的遗传研究—开花规律及其影响因素[J],浙江大学学报浓业与生命科学版),2001,27(1)：62～68
[64]覃鹏,刘飞虎,梁雪妮.超氧化物歧化酶与植物抗逆性.黑龙江农业科学,2002,(1)：31-34
[65]殷剑美,武耀廷,朱协习等.陆地棉产量与品质性状的主基因与多基因遗传分析[J].棉花学报,2003,25(2)：67-72
[66]余玲,丁勤,马翎健,宋喜悦,何蓓如.1B/1R与非1B/1R类型小麦雄性不育系SOD活性比较研究宁夏农学院学报,2003 1004-5260(2003)02-0001-02
[67]余叔文,汤章城.植物生理与分子生物学(第二版).北京：科学出版社,1999.722-723.
[68]喻树迅,范术丽,原日红,余学科,巩万奎.清除活性氧酶类对棉花早熟不早衰特性的遗传影响.棉花学报,1999,11(2)：100-105
[69]喻树迅,黄祯茂,姜瑞云,原日红,聂先舟,徐竹生,徐久玮.不同短季棉品种衰老过程生长机理的研究.作物学报,1994,20(5)：629-635
[70]喻树迅,张存信,黄祯茂.短季棉优质高产新技术[M].中国农业出版社,1998,PP.13-52
[71]喻树迅,张存信.中国短季棉概论[M].中国农业出版社,2003,PP.1-93
[72]喻树迅,范术丽,原日红等.清除活性氧酶类对棉花早熟不早衰持性的遗传影响[J].棉花学报,1999,11(2)：100-105
[73]喻树迅,袁有禄.数量性状遗传研究的新进展[J].棉花学报,2002,14(3)：150-154
[74]喻树迅,黄祯茂,姜瑞云等.不同短季棉品种衰老过程生化机理的研究[J].作物学报,1994,20(5)：629-636
[75]喻树迅,黄祯茂,姜瑞云等.短季棉中棉所16高产稳产生化[J1.中国农业科学,1992,25(5)：24-30
[76]喻树迅,黄祯茂,姜瑞云等.短季棉种子叶荧光动力学及50D酶活性的研究[J].中国农业科学,1993,26(3)：x4-20
[77]喻树迅,宋美珍,范术丽,原日红.短季棉早熟不早衰生化辅助育种技术研究[J].中国农业科学,2005,38(4)：664-670.
[78]喻树迅,黄祯茂.我国短季棉生产现状与发展前景[J].中国棉花,1989,16(2)：6-8
[79]喻树迅,黄祯茂.短季棉在我国农业生产中的地位[J].中国棉花,1991,18(3)：7-8
[80]喻树迅,黄祯茂.短季棉品种早熟性构成因素的遗传分析[J].中国农业科学,1990,23(6)：48-54
[81]喻树迅,宋美珍,范术丽,原日红.短季棉早熟不早衰生化辅助育种技术研究[J].中国农业科学,2005,38(4)：664-670.
[82]喻树迅.我国短季棉生产现状与发展前景[J].中国棉花,1989,16：(2)：6-8
[83]朱玉贤,李毅.现代分子生物学.北京：高等教育出版社,1997,11-12
[84]邹国林,罗时文,荣俊等.小白菜叶绿体铜锌超氧化物歧化酶的纯化和某些性质的研究.生物化学与生物物理学报,1989,21(1)：51
[85]邹国林,罗时文.一种小白菜叶细胞溶质铜锌超氧化物歧化酶的纯化和性质的研究.生物化学杂志,1989,5(2)：182
[86]赵会杰,刘华山等.小麦胞质不育系花粉败育与活性氧代谢关系的研究[J].作物学报,1996,22(3)：365-365
[87]赵伦一,陈舜文,徐世安.陆地棉早熟性的指标性状的遗传力估计[J].遗传学报,1974,1(1)：107-115
[88]赵伦一.陆地棉16种经济性状在不同选择强度下的遗传进度[J].遗传学报,1978,5(4)：315-321
[89]赵春江,康书江,王纪华等.植物内源激素对小麦叶片衰老的调控机理研究.华北农学报,2000,15(2)：53-56
[90]詹嘉红,蓝宗辉.外源ABA对低温胁迫水稻过氧化物酶同工酶的影响.生物技术,2003,13(1.7)7-9
[91]张建奎,宗学凤,王俊义,高东迎,余国东,石有明.温光敏核雄性不育小麦花药中保护酶活性的变化.麦类作物学报,2001,21(4)：26～30
[92]张建奎,何立人,李正伟等.温光型不育小麦C49S育性与花药SOD活性的关系[J],西南农业大学学报,1997,19(1)：56-581
[93]张海娜,李小娟,李存东,肖凯.过量表达小麦超氧化物歧化酶(SOD)基因对烟草耐盐能力的影响,作物学报,2008,34(8)：1403-1408
[94]覃鹏,孔治有,刘叶菊,刘飞虎.水分胁迫对烟草转SOD基因品系光合特性的影响,江苏农业学报,2004,20(2)：91～94
[95]Athanassios Molassiotis, Georgia Tanou, Grigorios Diamantidis, Angelos Patakas. Ioannis Therios Effects of 4-month Fe deficiency exposure on Fe reduction mechanism, photosynthetic gas exchange, chlorophyll fluorescence and antioxidant defense in two peach rootstocks differing in Fe deficiency tolerance. Journal of Plant Physiology,2006,163:176-185
[96]Bhatnagar-Mathur P, et al.Differential antioxidative responses in transgenic peanut bear no relationship to their superior transpiration efficiency under drought stress. J Plant Physiol, doi:10.1016/j.jplph.2009.01.001
[97]Chang-Hyo Goh, Seonghoe Jang, Sera Jung,AEHo-Seung, Kim Hong-Gyu, Kang Youn-il Park, Hyeun-Jong Bae, Choon-Hwan Lee Gynheung. An Rice photla mutation reduces plant growth by affecting photosynthetic responses to light during early seedling growt. Plant Mol Biol,2009, 69:605-619
[98]Chao Wang.,Song He Zhang, Pei Fang Wang, Jun Hou, Wen Jing Zhang, Wei Li, Zhi Ping Lin. The effect of excess Zn on mineral nutrition and antioxidative response in rapeseed seedlings.Chemosphere.2009,75:1468-1476
[99]Guo L-H, Wu X-L, Gong M. Roles of glutathione reductase and superoxide dismutase in heat-shockinduced cross adaptation in maize seedlings. Plant Physiol Commun,2005,41(4): 429-432
[100]HaihuaWang, TaoFeng, XixuPeng, MingliYan, XinkeTang.Up-regulation of chloroplastic antioxidant capacity is involved in alleviation of nickel toxicityof Zea mays L. by exogenous salicylic acid. Ecotoxicology and EnvironmentalSafety,2009,72:1354-1362
[101]Hee Jin Kim, Naohiro Kato,Sunran Kim, Barbara Triplett. Cu/Zn superoxide dismutases in developing cotton fibers:evidence for an extracellular form. Planta,2008,228:281-292
[102]H. Ozaki, M. Ikeuchi, T. Ogawa, H. Fukuzawa, K. Sonoik. Large-Scale analysis of chlorophyll fluorescence kinetics in Synechocystis identification of the factors involved in the modulation of photosystem stoichiometry, Plant Cell Physiol,2007,48:451-458.
[103]Islam MM, et al. Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells.2009, J Plant Physio., doi:10.1016/j.jplph.2009.04.002
[104]Jaromir Michalowicza, MalgorzataPosmykb. WirgiliuszDuda Chlorophenols induce lipid peroxidationand change antioxidant parameters in the leaves of wheat. Journal of PlantPhysiology,2009,166:559-568
[105]Jiang, L, Yang H.Prometryne-induced oxidative stress and impact on antioxidant enzymes in wheat. Ecotoxicol. Environ. Saf.,doi:10.1016/j.ecoenv.2009.04.025
[106]Khalid Amaria,Pedro Di'az-Vivancos,Vincente Palla's,Man'a Amelia,Sa'nchez-Pina,Jose' Antonio Herna'ndez. Oxidative stress induction by Prunus necrotic ringspot virus infection in apricot seeds. Physiologia Plantarum,2007,131:302-310.
[107]LAI Qi-xian, BAO Zhi-yil,ZHU Zhu-jun, QIAN Qiong-qiu, MAO Bi-zeng. Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified gerbera. Journal of Zhejiang University,SCIENCE B ISSN 1673-1581 (Print); ISSN 1862-1783
[108]Lixiong He, Yusuke Ban, Hiromichi Inoue, Narumi Matsuda, Jihong Liu. Takaya Moriguchi Enhancement of spermidine content and antioxidant capacity in transgenic pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis. Phytochemistry,2008,69:2133-2141
[109]Lin RZ, WangXR, Luo Y.Effectofsoilcadmiumongrowth, oxidative stress and antioxidant enzymes systemin wheat seedlings(Triticumaestivum L.). Chemosphere 2007,69:89-94.
[110]Manju Shri, Smita Kumar, Debasis Chakrabarty, Prabodh Kumar Trivedi, Shekhar Mallick, Prashant Misra, Devesh Shukla, Seema Mishra, Sudhakar Srivastava, Rudra D. Tripathi. Rakesh Tuli Effect of arsenic on growth, oxidative stress, and antioxidant system in rice seedlings. Ecotoxicology and Environmental Safety,2009,72:1102-1110
[111]Marta Vallino, Elena Martino, Francesca Boella, Claude Murat, Marco Chiapello, Silvia Perotto. Cu,Zn superoxide dismutase and zinc stress in themetal-tolerant Ericoidmy corrhizal fungus Oidiodendronmaius Zn FEMS.Microbiol Lett,2009,293:48-57
[112]McKersie, B.D, Bowley, S.R, and Jones. K.S.Winter survival of transgenic alfalfa overexpressing superoxide dismutase. Plant Physiol,1999,119:839-848.
[113]Menq Jiau Tseng, Cheng-Wei Liu, Jinn-Chin Yiu. Enhanced tolerance to sulfur dioxide and salt stress of transgenic Chinese cabbage plants expressing both superoxide dismutase and catalase in chloroplasts. Plant Physiology and Biochemistry,2007,45:822-833
[114]Miller AF. Superoxide dismutases:active sites that save, but a protein that kills. Curr Opin Chem Biol.2004,8:162-168
[115]Mieko Higuchi, Hiroshi Ozaki, Minami Matsui, Kintake Sonoike. A T-DNA insertion mutant of AtHMA1 gene encoding a Cu transporting ATPase inArabidopsis thaliana has a defect in the water-water cycle of photosynthesis.2008, Journal of Photochemistry and Photobiology, 94:205-213
[116]M.M. Posmyk, R. Kontek, K.M. Janas. Antioxidant enzymes activity and phenolic compounds contentin red cabbage seedlings exposed to copper stress. Ecotoxicology and Environmental Safety,2009,72:596-602
[117]Nadia M, Semchuk, Oleh V. Lushchak, Jon Falk, Karin Krupinska, Volodymyr I, Lushchak. Inactivation of genes, encoding tocopherol biosynthetic pathway enzymes,results in oxidative stress in outdoor grown Arabidopsis thalia.Plant Physiology and Biochemistry,2009,47:384-390
[118]Nicole Geissler, Sayed Hussin, Hans-Werner Koyro. Elevated atmospheric CO2 concentration ameliorates effects of NaCl salinity on photosynthesis and leaf structureof Aster tripolium L. Journal of Experimental Botany,2009, Vol.60, No.1, pp.137-151
[119]P. Diaz-Vivancos, M. Rubio, V. Mesonero, P. M. Periago, A. Ros Barcelo, P. Marti'nez-Go'mez, J. A. Herna'ndez. The apoplastic antioxidant system in Prunus:response to long-term plum pox virus infection. Journal of Experimental Botany,2006, Vol.57, No.14,3813-3824
[120]Peidong Jiang,Xiaoquan Zhang, Yunguo Zhu,Wei Zhu, Haiyan Xie, Xuede Wang. Metabolism of reactive oxygen species in cotton cytoplasmic male sterility and its restoration. Plant Cell Rep, 2007,26:1627-1634
[121]Ping He, Mitsuru Osaki, Masako Takebe, Takuro Shinano and Jun Wasaki. Endogenous hormones and expression of senescence-related genes in different senescenttypes of maize. Journal of Experimental Botany,2005, Vol.56, No.414,1117-1128
[122]Pitcher, L.H, Zilinskas, B.A. Overexpression of copper/zinc superoxide dismutase in the cytosol of transgenic tobacco confers partial resistance to ozone-induced foliar necrosis. Plant Physiol. 1996,110:583-588.
[123]Rajesh Kumar, Tewari Praveen,Kumar Soohyun, Kim Eun-Joo, Hahn Kee-Yoeup Paek. Nitric oxide retards xanthine oxidase-mediated superoxide anion generation in Phalaenopsis flower:an implication of NO in the senescence and oxidative stress regulation Plant Cell Rep,2009, 28:267-279
[124]Ramanjulu Sunkar, Avnish Kapoor, and Jian-Kang Zhu. Posttranscriptional Induction of Two Cu/Zn Superoxide Dismutase Genes in Arabidopsis Is Mediated by Downregulation of miR398 and Important for Oxidative Stress Tolerance. The Plant Cell,2006, Vol.18,2051-2065
[125]Sandalio LM, DalurzoHC, Go'mezM.,Romero-PuertasMC. Cadmium-induced changes in the growth and oxidative metabolism of pea plants. JExpBot,2001,52:2115-26
[126]Sen Gupta, A, Webb, R.P, Holaday, A.S, and Allen, R.D. Over-expression of superoxide dismutase protects plants from oxidative stress:Induction of ascorbate peroxidase in superoxide dismutase-overexpressing plants. Plant Physiol.1993,103:1067-1073.
[127]Songbi Chen, Natan Gollop, Bruria Heuer. Proteomic analysis of salt-stressed tomato (Solanumlycopersicum) seedlings:effect of genotype and exogenousapplication of glycinebetaine. Journal of Experimental Botany,2009, Vol.60, No.7, pp.2005-2019
[128]Stepien P, Klobus G. Antioxidant defense in the leaves of C3 and C4 plants under salinity stress, Physiologia Plantarum,2005,125:31-40
[129]S.Srivalli, R. Khanna-Chopra. Delayed wheat flag leaf senescence due to removal of spikelets is associated with increased activities of leaf antioxidant enzymes, reduced glutathione/oxidized glutathione ratio and oxidative damage to mitochondrial proteins, Plant Physiol. Biochem,2009, doi:10.1016/j.plaphy.2009.03.015
[130]T. Srinivasan, K. Raja Rajesh Kumar, G. Meur, P. B.Kirti. Heterologous expression of Arabidopsis NPR1 (AtNPR1)enhances oxidative stress tolerance in transgenic tobacco plants, Biotechnol Lett,2009,DOI 10.1007/s10529-009-0022-5
[131]Van Breusegem, F, Slooten, L, Stassart, J.M.,Moens, T, Botterman, J, Van Montagu, M and Inze, D. Overproduction of Arabidopsis thaliana FeSOD confers oxidative stress tolerance to transgenic maize. Plant Cell Physiol1999,40:515-523.
[132]Vivek Pandey, Vivek Dixit, Radhey Shyam. Chromium effect on ROS generation and detoxification in pea (Pisum sativum) leaf chloroplasts. Protoplasma,2009,236:85-95
[133]Wen-Bin Wang, Yun-Hee Kim, Haeng-Soon Lee, Ki-Yong Kim, Xi-Ping Deng, Sang-Soo Kwak. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiology and Biochemistry,2009,47:570-577
[134]Xing-Shun Song, Yan-Jie Wanga, Wei-Hua Maoa, Kai Shia, Yan-Hong Zhoua,Salvador Nogue' sd, Jing-Quan Yu.Effects of cucumber mosaic virus infection on electrontransport and antioxidant system in chloroplasts and mitochondria of cucumber and tomato leaves. Physiologia Plantarum,2009,135:246-257
[135]Xu Xiaojing, Zhou Yijun, Wei Shanjun, Ren Dongtao, Yang Min, Bu Huahu, Kang Mingming,Wang Junli, Feng Jinchao. Molecular Cloning and Expression of a Cu/Zn-Containing Superoxide Dismutase from Thellungiella halophila. Mol. Cells OT,2009,423-428
[136]Ya-Jun Cao, Qiang Wei,Yang Liao, He-Ling, Song Xian Li, Cheng-Bin Xiang, Ben-Ke Kuai. Ectopic overexpression of AtHDG11 in tall fescue resulted in enhanced tolerance to drought and salt stress, Plant Cell Rep,2009,28:579-588
[137]YanR, GaoS,YangW, CaoM, WangS, ChenF. Nickel toxicity induced antioxidant enzyme and phenylalanine ammonia-lyaseactivitiesin Jatropha curcas L. cotyledons. Plant Soil Environ, 2008,54:294-300.
[138]Yasemin Ekmekc, Deniz Tanyolac. Beycan Ayhan Effects of cadmium on antioxidant enzyme and photosynthetic activities in leaves of two maize cultivars. Journal of Plant Physiology,2008, 165:600-611
[139]YMING ZHANG, JIALI TAN, ZHENFEI GUO, SHAOYUN LU,SUIAN HE, WEI SHU, BIYAN ZHOU. Increased abscisic acid levels in transgenic tobaccoover-expressing 9 cis-epoxycarotenoid dioxygenase influence H2O2 and NO production and antioxidant defences,Plant, Cell and Environment,2009,32:509-519
[140]Yuqin Ke, Guoqiang Han, Huaqin He, Jiaxu Li. Differential regulation of proteins and phosphoproteins in rice under drought stress. Biochemical and Biophysical Research Communications,2009,379:133-138
[141]Zhao F, ZhangH. Salt and paraquat stress tolerance results from co-expression of the Suaeda salsa glutathione S-transferase and catalase in transgenic rice. Chemosphere,2006,86:349-57
[142]Zhou Y.H,Yu,J.Q,Mao,W.H,Huang,L.F,Song,X.S,Nogue's.Genotypic variation on Rubisco expression, photosynthetic electron flow and antioxidant metabolism in the chloroplasts of chill-expose dcucumberplants. PlantCell Physiol,2006,47:192-199
[2]程光宇,王峰,陶明煊,陆长梅,吴国荣,常福辰.聚丙烯酰胺梯度凝胶电泳和均一凝胶电泳在分离及鉴定植物SOD同工酶中的比较研究[J].南京师大学报(自然科学版),2008,1001-4616(2008)04-0097-07
[3]崔瑞峰,杜娟.甘蓝幼苗感染黑腐病后SOD、POD活性的变化[J].2009,34(5)：35—37,40
[4]陈锦云,林祥永,兰志斌等.超氧化物歧化酶防治烟草气候斑点病效果初报.福建农业科技,2006,(5)：13
[5]陈忠宁,毛宗万,唐雯霞.铜锌超氧化物歧化酶和结构、机理及其模拟研究进展.化学通报,1999,6：1-8
[6]程光宇,魏锦城,邹玉珍,吴国荣.拘祀果实Cu/Zn-SOD的纯化及其性质的研究.南京师范大学学报(自然科学版),1991,14(2)：82-92
[7]崔京日,黄世臣.水分胁迫及接种VAM对山杏叶片SOD活性和MDA含量变化的影响[J].延边大学农学学报,2009,31(2)：84-88
[8]戴鑫,张边江,章琦,毛善国.盐胁迫下不同浓度的锰处理对小麦幼苗生长及SOD.POD的影响[J].安徽农业科学.2009,37(27)：13000-13001
[9]耿军义,张香云,崔瑞敏等.杂交棉冀棉18号一代、二代的SOD和POD活性及生理生化机制研究[J].华北农学报,2002,17(4)：96-99
[10]高学曾,许金芳等.玉米正常粒和败育粒淀粉磷酸化酶、过氧化酶活必及其同工短季棉早熟不早衰生化机制及遗传研究酶的比较[J].植物生理学通讯,2007,(4)：30-35
[11]高俊杰,秦爱国,于贤昌.低温胁迫下嫁接对黄瓜叶片SOD和CAT基因表达与活性变化的影响.应用生态学报,2009,20(1)：213-217
[12]葛知男.陆地棉早熟性的生理特点及其遗传研究[J].江苏农业科学,1993,(1)：21-24
[13]郭海军,董志强,林永增等.黄萎病对棉花叶片SOD、POD酶活性和光合特性的影响[J].中国农业科学,2006,25(6)：40-46
[14]贺鹏,朱列书,钟波.航天诱变对烤烟大田期SOD活性的影响[J].中国农学通报,2008,24(5)：123-125。
[15]黄绍兴,阎隆飞.植物发育过程中特异基因的表达[J].植物生理学通讯,1995,31(30)：161-166
[16]李大辉.铅污染对菱幼苗SOD、POD及叶细胞亚显微结构的影响。中国环境科学,2009,29(2)：136-141
[17]李筠,邓西平,郭尚洙,田中净.转铜/锌超氧化物歧化酶和抗坏血酸过氧化物酶基因甘薯的耐旱性,植物生理与分子生物学学报,2006,32(4)：451-457
[18]龙书生,曹远林,李亚玲,康振生.小麦抗条锈病过敏性坏死反应中的活性氧代谢[J].西北农林 科技大学学报(自然科学版),2009,11：125-130
[19]林庆斌,丁杨,王海波,张昭其,毛小云,乐学义.盐胁迫下SOD模拟物对水稻幼苗生物量及SOD活性的影响[J].土壤通报.2009,5：1163-1166
[20]罗广华,王爱国.植物SOD的凝胶电泳及活性显示[J].植物生理学通讯,1983,(6)：44-45.
[21]罗广华,王爱国.植物SOD同工酶活性显示的某些干扰[J].植物生理学通讯,1993,29(2)：119-122.
[22]吉文成,周有耀.棉花早熟性与纤维性状的遗传分析[J].北京农业大学学报,1994,20(4)：389-396
[23]蒋选利,李振岐,康振生.过氧化物酶与植物抗病性研究进展.西北农林科技大学学报(自然科学版),2001,29(6)：124-129
[24]姜瑞云,王宏,黄祯茂等.浅谈棉花早衰[J].中国棉花,1996,23(3)：7-8
[25]刘凤权,王金生.水杨酸对水稻叶片抗氧化酶系的影响[J].农业生物技术学报,2001,9(4)：396-399
[26]马淑娟,喻树迅,范术丽,宋美珍.转棉花叶绿体Cu/Zn-SOD基因烟草的获得及其功能的初步验证分子植物育种,2007,Vol.5,No.3,319-323
[27]潘家驹,阂留芳,吴振衡二陆地棉若干性状之间的遗传相关及遗传力的初步研究[J].江苏农业科学,1979,(4)：1-7
[28]潘家驹.作物育种学总论[M].中国农业出版社,1998
[29]潘家驹.棉花育种学[M].中国农业出版社,1995,p.273-295
[30]潘哲超,陈建斌,范静华,周惠萍,邱世刚,叶漪,果志华.稻瘟菌粗毒素对水稻防御性相关酶系的诱导.云南农业大学学报,2008,1004-390X(2008)02-0162-05
[31]秦水琼,贾士荣.植物抗氧化逆境的基因下程.农业生物技术学报,1997,5(1)：15-22
[32]盛良全,郑晓云,门向阳,肖厚荣,厦炳乐,刘少民.生命体中的超氧化物歧化酶.医学基础与药学研究,2002,48(6)：48-53
[33]石春海,吴建国,樊龙江,朱军,吴平.不同环境条件下稻米透明度的发育遗传研究分析[J].遗传学报,2002,29(1)：56-61
[34]汤学军,王康.激动素和抗坏血酸保护受冷害苗细胞膜和促进SOD合成的效应[J].植物学报,1993,(增刊)：45-49
[35]唐薇,李维江,张冬梅等.干旱对转基因抗虫棉苗期叶片POD、MDA和光合速率的影响[J].中国棉花,2002,29(2)：23-24
[36]陶均,李玲.高等植物脱落酸生物合成的酶调控.植物学通报,2002,19(6)：675-683
[37]田志刚,张淑芳,丁智明.短季棉株型性状与经济性状关系初探[J].中国棉花,1999,26(2)：18-19
[38]田志刚,郝笑琦,张淑芳.短季棉早熟性状及产量构成因素的遗传分析和选择策略[J],华北农学报,1993,8(1)：18-22
[39]田蓄华.早熟陆地棉主要性状的遗传率及遗传进度的研究[J].遗传,1983,5(1)：15～16
[40]万永吉,郑文教,方煜,王铮敏,邱瑾.重金属铬(Ⅲ)胁迫对红树植物秋茄幼苗SOD、POD活性 及其同工酶的影响,厦门大学学报,2008,0438-0479(2008)04-0571-04)
[41]魏玉香,李永红,谷茂,刘晓辉.鸡冠花幼苗热胁迫耐性与其SOD之间的关联.西北植物学报,2008,28(10)：2055-2061
[42]王兆龙,曹卫星.细胞分裂素对植物基因表达的调节[J].植物生理学通讯,2000,36(1)：82-88
[43]王国山,顾恒兰,毕淑兰.短季棉主要性状遗传分析与改良[J].沈阳农业大学学报,1998,29(1)：6-1
[44]王国印.陆地棉数量性状遗传研究与进展[J].中国棉花,1994,21(3)：5-7
[45]王建华,刘鸿先,徐同等.超氧化物岐化酶SOD在植物逆境和衰老生理中的作用[J].植物生理学通讯,1959,25(1)：1-7
[46]王忠军,陈爱民,王沛政等.陆地棉主要经济性状的配合力和遗传力分析[J].新疆农业大学学报,1999,22(一)：69-72
[47]王莹,戴继勋.裙带菜同工酶酶谱特征及其遗传多样性的研究.遗传学报,1999,26(3)269～273
[48]王雅平,刘伊强,施磊等.小麦对赤霉病抗性不同品种的50D活性[J].植物生短季棉早熟不早衰生化机制及遗传研究理学报,1993,19(4)：353-358
[49]王淼,李秋荣,付士磊,董百丽.外源一氧化氮对干旱胁迫下杨树光合作用的影响,应用生态学报2005,16(2)：218～222
[50]王璞,陈咖.植物激素脱落酸的信号转导[J].植物生理学通讯,2000,36(2)：165-169
[51]温伟庆,陈友吾.银杏雌雄株过氧化物酶和过氧化氢酶活性差异研究.福建林业科技,2002,29(2)：34-39
[52]许长成,邹琦.大豆叶片旱促衰老及其与膜脂过氧化的关系[J].作物学报,1993,19(4)：360-364
[53]许长成,赵世杰,邹奇.植物组织内丙二醛的分离与鉴定[J1.植物生理学通讯,1992,28(4)：189-198
[54]汤章城.植物对水分胁迫的反应和适应性n.植物对干旱的反应和适应性.植物生理学通讯,1983,(4)：1-7
[55]王关林,方宏绮.植物基因工程(第二版).北京：科学出版社,2002：527-528
[56]文才艺,吴元华,李浩戈等.接种PVYN后烟草叶片SOD活性和MDA含量的变化.中国烟草科学,1999,(1)：12-14
[57]徐德利,宋平.大麦感染黄花叶病后POD、SOD活性变化规律的初步研究[J]。南京师大学报(自然科学版),2008,31(2)：101-104
[58]孙天国,沙伟.盐胁迫对甜瓜种子萌发及幼苗SOD.POD活性的影响[J].安徽农业科学,2009,37(24)：11509-11510,11513
[59]闰新甫.全球转基因作物种植概况.世界农业,2001,4：22-3
[60]杨答,邹国林,朱汝番.鸭血超氧化物歧化酶的纯化及性质研究.生物化学杂志.1989,5(2)：169
[61]姚广,高辉远,王未未,张立涛,部建雯,铅胁迫对玉米幼苗叶片光系统功能及光合作用的影响.生态学报,2009,29(3)：1162～1169.
[62]叶子弘,朱军.陆地棉开花成铃性状的遗传研究-不同发育阶段的遗传规律[J].遗传学报,2000,27(9)：800-809
[63]叶子弘,朱军.陆地棉开花成铃性状的遗传研究—开花规律及其影响因素[J],浙江大学学报浓业与生命科学版),2001,27(1)：62～68
[64]覃鹏,刘飞虎,梁雪妮.超氧化物歧化酶与植物抗逆性.黑龙江农业科学,2002,(1)：31-34
[65]殷剑美,武耀廷,朱协习等.陆地棉产量与品质性状的主基因与多基因遗传分析[J].棉花学报,2003,25(2)：67-72
[66]余玲,丁勤,马翎健,宋喜悦,何蓓如.1B/1R与非1B/1R类型小麦雄性不育系SOD活性比较研究宁夏农学院学报,2003 1004-5260(2003)02-0001-02
[67]余叔文,汤章城.植物生理与分子生物学(第二版).北京：科学出版社,1999.722-723.
[68]喻树迅,范术丽,原日红,余学科,巩万奎.清除活性氧酶类对棉花早熟不早衰特性的遗传影响.棉花学报,1999,11(2)：100-105
[69]喻树迅,黄祯茂,姜瑞云,原日红,聂先舟,徐竹生,徐久玮.不同短季棉品种衰老过程生长机理的研究.作物学报,1994,20(5)：629-635
[70]喻树迅,张存信,黄祯茂.短季棉优质高产新技术[M].中国农业出版社,1998,PP.13-52
[71]喻树迅,张存信.中国短季棉概论[M].中国农业出版社,2003,PP.1-93
[72]喻树迅,范术丽,原日红等.清除活性氧酶类对棉花早熟不早衰持性的遗传影响[J].棉花学报,1999,11(2)：100-105
[73]喻树迅,袁有禄.数量性状遗传研究的新进展[J].棉花学报,2002,14(3)：150-154
[74]喻树迅,黄祯茂,姜瑞云等.不同短季棉品种衰老过程生化机理的研究[J].作物学报,1994,20(5)：629-636
[75]喻树迅,黄祯茂,姜瑞云等.短季棉中棉所16高产稳产生化[J1.中国农业科学,1992,25(5)：24-30
[76]喻树迅,黄祯茂,姜瑞云等.短季棉种子叶荧光动力学及50D酶活性的研究[J].中国农业科学,1993,26(3)：x4-20
[77]喻树迅,宋美珍,范术丽,原日红.短季棉早熟不早衰生化辅助育种技术研究[J].中国农业科学,2005,38(4)：664-670.
[78]喻树迅,黄祯茂.我国短季棉生产现状与发展前景[J].中国棉花,1989,16(2)：6-8
[79]喻树迅,黄祯茂.短季棉在我国农业生产中的地位[J].中国棉花,1991,18(3)：7-8
[80]喻树迅,黄祯茂.短季棉品种早熟性构成因素的遗传分析[J].中国农业科学,1990,23(6)：48-54
[81]喻树迅,宋美珍,范术丽,原日红.短季棉早熟不早衰生化辅助育种技术研究[J].中国农业科学,2005,38(4)：664-670.
[82]喻树迅.我国短季棉生产现状与发展前景[J].中国棉花,1989,16：(2)：6-8
[83]朱玉贤,李毅.现代分子生物学.北京：高等教育出版社,1997,11-12
[84]邹国林,罗时文,荣俊等.小白菜叶绿体铜锌超氧化物歧化酶的纯化和某些性质的研究.生物化学与生物物理学报,1989,21(1)：51
[85]邹国林,罗时文.一种小白菜叶细胞溶质铜锌超氧化物歧化酶的纯化和性质的研究.生物化学杂志,1989,5(2)：182
[86]赵会杰,刘华山等.小麦胞质不育系花粉败育与活性氧代谢关系的研究[J].作物学报,1996,22(3)：365-365
[87]赵伦一,陈舜文,徐世安.陆地棉早熟性的指标性状的遗传力估计[J].遗传学报,1974,1(1)：107-115
[88]赵伦一.陆地棉16种经济性状在不同选择强度下的遗传进度[J].遗传学报,1978,5(4)：315-321
[89]赵春江,康书江,王纪华等.植物内源激素对小麦叶片衰老的调控机理研究.华北农学报,2000,15(2)：53-56
[90]詹嘉红,蓝宗辉.外源ABA对低温胁迫水稻过氧化物酶同工酶的影响.生物技术,2003,13(1.7)7-9
[91]张建奎,宗学凤,王俊义,高东迎,余国东,石有明.温光敏核雄性不育小麦花药中保护酶活性的变化.麦类作物学报,2001,21(4)：26～30
[92]张建奎,何立人,李正伟等.温光型不育小麦C49S育性与花药SOD活性的关系[J],西南农业大学学报,1997,19(1)：56-581
[93]张海娜,李小娟,李存东,肖凯.过量表达小麦超氧化物歧化酶(SOD)基因对烟草耐盐能力的影响,作物学报,2008,34(8)：1403-1408
[94]覃鹏,孔治有,刘叶菊,刘飞虎.水分胁迫对烟草转SOD基因品系光合特性的影响,江苏农业学报,2004,20(2)：91～94
[95]Athanassios Molassiotis, Georgia Tanou, Grigorios Diamantidis, Angelos Patakas. Ioannis Therios Effects of 4-month Fe deficiency exposure on Fe reduction mechanism, photosynthetic gas exchange, chlorophyll fluorescence and antioxidant defense in two peach rootstocks differing in Fe deficiency tolerance. Journal of Plant Physiology,2006,163:176-185
[96]Bhatnagar-Mathur P, et al.Differential antioxidative responses in transgenic peanut bear no relationship to their superior transpiration efficiency under drought stress. J Plant Physiol, doi:10.1016/j.jplph.2009.01.001
[97]Chang-Hyo Goh, Seonghoe Jang, Sera Jung,AEHo-Seung, Kim Hong-Gyu, Kang Youn-il Park, Hyeun-Jong Bae, Choon-Hwan Lee Gynheung. An Rice photla mutation reduces plant growth by affecting photosynthetic responses to light during early seedling growt. Plant Mol Biol,2009, 69:605-619
[98]Chao Wang.,Song He Zhang, Pei Fang Wang, Jun Hou, Wen Jing Zhang, Wei Li, Zhi Ping Lin. The effect of excess Zn on mineral nutrition and antioxidative response in rapeseed seedlings.Chemosphere.2009,75:1468-1476
[99]Guo L-H, Wu X-L, Gong M. Roles of glutathione reductase and superoxide dismutase in heat-shockinduced cross adaptation in maize seedlings. Plant Physiol Commun,2005,41(4): 429-432
[100]HaihuaWang, TaoFeng, XixuPeng, MingliYan, XinkeTang.Up-regulation of chloroplastic antioxidant capacity is involved in alleviation of nickel toxicityof Zea mays L. by exogenous salicylic acid. Ecotoxicology and EnvironmentalSafety,2009,72:1354-1362
[101]Hee Jin Kim, Naohiro Kato,Sunran Kim, Barbara Triplett. Cu/Zn superoxide dismutases in developing cotton fibers:evidence for an extracellular form. Planta,2008,228:281-292
[102]H. Ozaki, M. Ikeuchi, T. Ogawa, H. Fukuzawa, K. Sonoik. Large-Scale analysis of chlorophyll fluorescence kinetics in Synechocystis identification of the factors involved in the modulation of photosystem stoichiometry, Plant Cell Physiol,2007,48:451-458.
[103]Islam MM, et al. Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells.2009, J Plant Physio., doi:10.1016/j.jplph.2009.04.002
[104]Jaromir Michalowicza, MalgorzataPosmykb. WirgiliuszDuda Chlorophenols induce lipid peroxidationand change antioxidant parameters in the leaves of wheat. Journal of PlantPhysiology,2009,166:559-568
[105]Jiang, L, Yang H.Prometryne-induced oxidative stress and impact on antioxidant enzymes in wheat. Ecotoxicol. Environ. Saf.,doi:10.1016/j.ecoenv.2009.04.025
[106]Khalid Amaria,Pedro Di'az-Vivancos,Vincente Palla's,Man'a Amelia,Sa'nchez-Pina,Jose' Antonio Herna'ndez. Oxidative stress induction by Prunus necrotic ringspot virus infection in apricot seeds. Physiologia Plantarum,2007,131:302-310.
[107]LAI Qi-xian, BAO Zhi-yil,ZHU Zhu-jun, QIAN Qiong-qiu, MAO Bi-zeng. Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified gerbera. Journal of Zhejiang University,SCIENCE B ISSN 1673-1581 (Print); ISSN 1862-1783
[108]Lixiong He, Yusuke Ban, Hiromichi Inoue, Narumi Matsuda, Jihong Liu. Takaya Moriguchi Enhancement of spermidine content and antioxidant capacity in transgenic pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis. Phytochemistry,2008,69:2133-2141
[109]Lin RZ, WangXR, Luo Y.Effectofsoilcadmiumongrowth, oxidative stress and antioxidant enzymes systemin wheat seedlings(Triticumaestivum L.). Chemosphere 2007,69:89-94.
[110]Manju Shri, Smita Kumar, Debasis Chakrabarty, Prabodh Kumar Trivedi, Shekhar Mallick, Prashant Misra, Devesh Shukla, Seema Mishra, Sudhakar Srivastava, Rudra D. Tripathi. Rakesh Tuli Effect of arsenic on growth, oxidative stress, and antioxidant system in rice seedlings. Ecotoxicology and Environmental Safety,2009,72:1102-1110
[111]Marta Vallino, Elena Martino, Francesca Boella, Claude Murat, Marco Chiapello, Silvia Perotto. Cu,Zn superoxide dismutase and zinc stress in themetal-tolerant Ericoidmy corrhizal fungus Oidiodendronmaius Zn FEMS.Microbiol Lett,2009,293:48-57
[112]McKersie, B.D, Bowley, S.R, and Jones. K.S.Winter survival of transgenic alfalfa overexpressing superoxide dismutase. Plant Physiol,1999,119:839-848.
[113]Menq Jiau Tseng, Cheng-Wei Liu, Jinn-Chin Yiu. Enhanced tolerance to sulfur dioxide and salt stress of transgenic Chinese cabbage plants expressing both superoxide dismutase and catalase in chloroplasts. Plant Physiology and Biochemistry,2007,45:822-833
[114]Miller AF. Superoxide dismutases:active sites that save, but a protein that kills. Curr Opin Chem Biol.2004,8:162-168
[115]Mieko Higuchi, Hiroshi Ozaki, Minami Matsui, Kintake Sonoike. A T-DNA insertion mutant of AtHMA1 gene encoding a Cu transporting ATPase inArabidopsis thaliana has a defect in the water-water cycle of photosynthesis.2008, Journal of Photochemistry and Photobiology, 94:205-213
[116]M.M. Posmyk, R. Kontek, K.M. Janas. Antioxidant enzymes activity and phenolic compounds contentin red cabbage seedlings exposed to copper stress. Ecotoxicology and Environmental Safety,2009,72:596-602
[117]Nadia M, Semchuk, Oleh V. Lushchak, Jon Falk, Karin Krupinska, Volodymyr I, Lushchak. Inactivation of genes, encoding tocopherol biosynthetic pathway enzymes,results in oxidative stress in outdoor grown Arabidopsis thalia.Plant Physiology and Biochemistry,2009,47:384-390
[118]Nicole Geissler, Sayed Hussin, Hans-Werner Koyro. Elevated atmospheric CO2 concentration ameliorates effects of NaCl salinity on photosynthesis and leaf structureof Aster tripolium L. Journal of Experimental Botany,2009, Vol.60, No.1, pp.137-151
[119]P. Diaz-Vivancos, M. Rubio, V. Mesonero, P. M. Periago, A. Ros Barcelo, P. Marti'nez-Go'mez, J. A. Herna'ndez. The apoplastic antioxidant system in Prunus:response to long-term plum pox virus infection. Journal of Experimental Botany,2006, Vol.57, No.14,3813-3824
[120]Peidong Jiang,Xiaoquan Zhang, Yunguo Zhu,Wei Zhu, Haiyan Xie, Xuede Wang. Metabolism of reactive oxygen species in cotton cytoplasmic male sterility and its restoration. Plant Cell Rep, 2007,26:1627-1634
[121]Ping He, Mitsuru Osaki, Masako Takebe, Takuro Shinano and Jun Wasaki. Endogenous hormones and expression of senescence-related genes in different senescenttypes of maize. Journal of Experimental Botany,2005, Vol.56, No.414,1117-1128
[122]Pitcher, L.H, Zilinskas, B.A. Overexpression of copper/zinc superoxide dismutase in the cytosol of transgenic tobacco confers partial resistance to ozone-induced foliar necrosis. Plant Physiol. 1996,110:583-588.
[123]Rajesh Kumar, Tewari Praveen,Kumar Soohyun, Kim Eun-Joo, Hahn Kee-Yoeup Paek. Nitric oxide retards xanthine oxidase-mediated superoxide anion generation in Phalaenopsis flower:an implication of NO in the senescence and oxidative stress regulation Plant Cell Rep,2009, 28:267-279
[124]Ramanjulu Sunkar, Avnish Kapoor, and Jian-Kang Zhu. Posttranscriptional Induction of Two Cu/Zn Superoxide Dismutase Genes in Arabidopsis Is Mediated by Downregulation of miR398 and Important for Oxidative Stress Tolerance. The Plant Cell,2006, Vol.18,2051-2065
[125]Sandalio LM, DalurzoHC, Go'mezM.,Romero-PuertasMC. Cadmium-induced changes in the growth and oxidative metabolism of pea plants. JExpBot,2001,52:2115-26
[126]Sen Gupta, A, Webb, R.P, Holaday, A.S, and Allen, R.D. Over-expression of superoxide dismutase protects plants from oxidative stress:Induction of ascorbate peroxidase in superoxide dismutase-overexpressing plants. Plant Physiol.1993,103:1067-1073.
[127]Songbi Chen, Natan Gollop, Bruria Heuer. Proteomic analysis of salt-stressed tomato (Solanumlycopersicum) seedlings:effect of genotype and exogenousapplication of glycinebetaine. Journal of Experimental Botany,2009, Vol.60, No.7, pp.2005-2019
[128]Stepien P, Klobus G. Antioxidant defense in the leaves of C3 and C4 plants under salinity stress, Physiologia Plantarum,2005,125:31-40
[129]S.Srivalli, R. Khanna-Chopra. Delayed wheat flag leaf senescence due to removal of spikelets is associated with increased activities of leaf antioxidant enzymes, reduced glutathione/oxidized glutathione ratio and oxidative damage to mitochondrial proteins, Plant Physiol. Biochem,2009, doi:10.1016/j.plaphy.2009.03.015
[130]T. Srinivasan, K. Raja Rajesh Kumar, G. Meur, P. B.Kirti. Heterologous expression of Arabidopsis NPR1 (AtNPR1)enhances oxidative stress tolerance in transgenic tobacco plants, Biotechnol Lett,2009,DOI 10.1007/s10529-009-0022-5
[131]Van Breusegem, F, Slooten, L, Stassart, J.M.,Moens, T, Botterman, J, Van Montagu, M and Inze, D. Overproduction of Arabidopsis thaliana FeSOD confers oxidative stress tolerance to transgenic maize. Plant Cell Physiol1999,40:515-523.
[132]Vivek Pandey, Vivek Dixit, Radhey Shyam. Chromium effect on ROS generation and detoxification in pea (Pisum sativum) leaf chloroplasts. Protoplasma,2009,236:85-95
[133]Wen-Bin Wang, Yun-Hee Kim, Haeng-Soon Lee, Ki-Yong Kim, Xi-Ping Deng, Sang-Soo Kwak. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiology and Biochemistry,2009,47:570-577
[134]Xing-Shun Song, Yan-Jie Wanga, Wei-Hua Maoa, Kai Shia, Yan-Hong Zhoua,Salvador Nogue' sd, Jing-Quan Yu.Effects of cucumber mosaic virus infection on electrontransport and antioxidant system in chloroplasts and mitochondria of cucumber and tomato leaves. Physiologia Plantarum,2009,135:246-257
[135]Xu Xiaojing, Zhou Yijun, Wei Shanjun, Ren Dongtao, Yang Min, Bu Huahu, Kang Mingming,Wang Junli, Feng Jinchao. Molecular Cloning and Expression of a Cu/Zn-Containing Superoxide Dismutase from Thellungiella halophila. Mol. Cells OT,2009,423-428
[136]Ya-Jun Cao, Qiang Wei,Yang Liao, He-Ling, Song Xian Li, Cheng-Bin Xiang, Ben-Ke Kuai. Ectopic overexpression of AtHDG11 in tall fescue resulted in enhanced tolerance to drought and salt stress, Plant Cell Rep,2009,28:579-588
[137]YanR, GaoS,YangW, CaoM, WangS, ChenF. Nickel toxicity induced antioxidant enzyme and phenylalanine ammonia-lyaseactivitiesin Jatropha curcas L. cotyledons. Plant Soil Environ, 2008,54:294-300.
[138]Yasemin Ekmekc, Deniz Tanyolac. Beycan Ayhan Effects of cadmium on antioxidant enzyme and photosynthetic activities in leaves of two maize cultivars. Journal of Plant Physiology,2008, 165:600-611
[139]YMING ZHANG, JIALI TAN, ZHENFEI GUO, SHAOYUN LU,SUIAN HE, WEI SHU, BIYAN ZHOU. Increased abscisic acid levels in transgenic tobaccoover-expressing 9 cis-epoxycarotenoid dioxygenase influence H2O2 and NO production and antioxidant defences,Plant, Cell and Environment,2009,32:509-519
[140]Yuqin Ke, Guoqiang Han, Huaqin He, Jiaxu Li. Differential regulation of proteins and phosphoproteins in rice under drought stress. Biochemical and Biophysical Research Communications,2009,379:133-138
[141]Zhao F, ZhangH. Salt and paraquat stress tolerance results from co-expression of the Suaeda salsa glutathione S-transferase and catalase in transgenic rice. Chemosphere,2006,86:349-57
[142]Zhou Y.H,Yu,J.Q,Mao,W.H,Huang,L.F,Song,X.S,Nogue's.Genotypic variation on Rubisco expression, photosynthetic electron flow and antioxidant metabolism in the chloroplasts of chill-expose dcucumberplants. PlantCell Physiol,2006,47:192-199