对虾白斑综合症病毒性质和蛋白激酶wsv083的研究
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摘要
对虾白斑综合症病毒(white spot syndrome virus, WSSV)是一种杆状DNA病毒，具有囊膜和被膜，DNA为双链环状。WSSV是目前对虾的最主要的病害，对对虾养殖业造成了巨大危害。所以对WSSV的性质和感染机制等研究对于对虾养殖业意义重大。
本研究的主要内容如下：
①测定WSSV粒子的质量和其基本组成；
②探讨微生物在感染了WSSV的螯虾死亡过程中所起的作用；
③研究了WSSV囊膜表面的蛋白的种类；
④对WSSV的一个蛋白激酶wsv083进行了初步研究。
结果如下：
1、通过先计算出WSSV基因组的质量，再测定WSSV的基因组DNA占整个WSSV粒子质量的比例的思路，计算出了一个完整WSSV粒子的质量。进一步测定得到WSSV悬液的每个OD600相当于3.34×108/l的病毒浓度。另外，WSSV各组分在总重量中的比例经测定如下：基因组DNA占10.17%，核衣壳中的蛋白占43.07%，囊膜中的脂类含量约为22%，囊膜中的蛋白质含量约为24.76%；另外还测定了四种主要膜蛋白的相对比例。
2、为了研究微生物在感染了WSSV的克氏原螯虾(Procambarus clarkii)的死亡过程中的作用，对染毒螯虾注射抗生素，发现其死亡率与对照组相比有明显下降。平板计数结果显示染毒螯虾的血淋巴中微生物数量有明显增加。分析表明主要是嗜水气单胞菌（Aeromonas hydrophila）、弗劳地柠檬酸杆菌（Citrobacter freundii）、嗜麦芽窄食单胞菌（Stenotrophomonasmaltophilia）和琼氏不动杆菌（Acinetobacter junii）等条件致病菌。而抗生素对WSSV的增殖没有显著影响。研究结果表明螯虾在感染了WSSV后血细胞的消失导致体内病原微生物的数量大幅增加，从而加速了螯虾的死亡速度。
3、利用生物素标记转移技术对牛血清白蛋白（BSA）和凡纳滨对虾的鳃细胞膜蛋白进行标记，研究其与对虾白斑综合症病毒（WSSV）之间的相互作用。发现分子量在50-56KD之间的两种蛋白质位于WSSV的表面，并且与鳃细胞膜蛋白具有特异性的相互作用。根据分子量推测可能是病毒的两种膜蛋白，VP52A和VP56。
4、把wsv083在大肠杆菌中进行表达和纯化，与纯化的VP28和VP19置于激酶缓冲液中看能否催化磷酸化。研究结果表明wsv083并不具备这个功能。另外还发现wsv083存在苏氨酸和酪氨酸的磷酸化，但没有丝氨酸的磷酸化。而wsv083的突变体则没有磷酸化。结果表明wsv083可以催化自身磷酸化。但其具体功能还需进一步研究。
Shrimp white spot syndrome virus (white spot syndrome virus, WSSV) is arod-shaped DNA virus, with the envelop and the tegument, DNA as double chainring. WSSV is currently the main diseases of prawn aquaculture, caused massiveharm. Therefore, the study on properties and infection mechanism of WSSV is ofgreat significance for prawn aquaculture.
The main content of this study are as follows:
①determination of WSSV particle mass and its basic components;
②investigate the role of the microbial infection in the death of crayfishinfected with WSSV;
③the identification of the WSSV particle surface protein;
④a WSSV protein kinase wsv083were studied.
The results are as follows:
First the mass of WSSV genome was calculated, and the proportion of thegenomic DNA accounted for total WSSV particle virus mass was also measured,finally calculate the mass of a complete WSSV particle. The relative proportionsof the major envelop protein were also studied. WSSV suspension for each of theOD600was equivalent to3.34×108/L of the virus concentration.In addition,WSSV components in total weight in proportion through the determination wereas follows: the genomic DNA accounted for10.17%, nucleocapsid protein is43.07%, envelop lipid content is22%, envelop protein content is24.76%.
To study the role of microorganism in the process of death of theProcambarus clarkia infected with WSSV(white spot syndrome virus), themortality rate of Procambarus clarkia injected with antibiotics decreasedsignificantly compared with the control group. Plate count showed that thehemolymph of Procambarus clarkia infected with WSSV have significantly increased the number of microorganisms. Analysis showed that they wereAeromonas hydrophila, Citrobacter freundii, Stenotrophomonas maltophilia andAcinetobacter junii. All of them were opportunistic pathogen. Antibiotic had nosignificant effect on the WSSV’s proliferation.The results showed that thedisappearance of the blood cells of Procambarus clarkia infected with WSSV invivo led to a substantial increase in the number of pathogenic microorganisms,thereby accelerating the rate of Procambarus clarkia deaths.
Using the biotin label transfer technique, we found that the biotin label wastransferred from BSA or gill member protein of Litopenaeus vannamei to theenvelop protein of WSSV. Western blotting showed that two proteins whosemolecular weight among50-55KD were labeled with biotin. It was concludedthat the two proteins was location on the surface of the WSSV viron and couldinteract with the gill member protein of Litopenaeus vannamei. They wereestimated to be VP52A and VP56.
VP28、VP19and wsv083were expressed in E. coli and purified. They wereput in kinase buffer to learn if wsv083can catalyze VP28and VP19’sphosphorylation. Results showed that wsv083cann’t. We also found that therewere threonine and tyrosine phosphorylation but no serine phosphorylation inwsv083. And wsv083mutant was not phosphorylated. It was concluded thatwsv083catalyze autophosphorylation. But its specific function needs furtherstudy.
本研究的主要内容如下：
①测定WSSV粒子的质量和其基本组成；
②探讨微生物在感染了WSSV的螯虾死亡过程中所起的作用；
③研究了WSSV囊膜表面的蛋白的种类；
④对WSSV的一个蛋白激酶wsv083进行了初步研究。
结果如下：
1、通过先计算出WSSV基因组的质量，再测定WSSV的基因组DNA占整个WSSV粒子质量的比例的思路，计算出了一个完整WSSV粒子的质量。进一步测定得到WSSV悬液的每个OD600相当于3.34×108/l的病毒浓度。另外，WSSV各组分在总重量中的比例经测定如下：基因组DNA占10.17%，核衣壳中的蛋白占43.07%，囊膜中的脂类含量约为22%，囊膜中的蛋白质含量约为24.76%；另外还测定了四种主要膜蛋白的相对比例。
2、为了研究微生物在感染了WSSV的克氏原螯虾(Procambarus clarkii)的死亡过程中的作用，对染毒螯虾注射抗生素，发现其死亡率与对照组相比有明显下降。平板计数结果显示染毒螯虾的血淋巴中微生物数量有明显增加。分析表明主要是嗜水气单胞菌（Aeromonas hydrophila）、弗劳地柠檬酸杆菌（Citrobacter freundii）、嗜麦芽窄食单胞菌（Stenotrophomonasmaltophilia）和琼氏不动杆菌（Acinetobacter junii）等条件致病菌。而抗生素对WSSV的增殖没有显著影响。研究结果表明螯虾在感染了WSSV后血细胞的消失导致体内病原微生物的数量大幅增加，从而加速了螯虾的死亡速度。
3、利用生物素标记转移技术对牛血清白蛋白（BSA）和凡纳滨对虾的鳃细胞膜蛋白进行标记，研究其与对虾白斑综合症病毒（WSSV）之间的相互作用。发现分子量在50-56KD之间的两种蛋白质位于WSSV的表面，并且与鳃细胞膜蛋白具有特异性的相互作用。根据分子量推测可能是病毒的两种膜蛋白，VP52A和VP56。
4、把wsv083在大肠杆菌中进行表达和纯化，与纯化的VP28和VP19置于激酶缓冲液中看能否催化磷酸化。研究结果表明wsv083并不具备这个功能。另外还发现wsv083存在苏氨酸和酪氨酸的磷酸化，但没有丝氨酸的磷酸化。而wsv083的突变体则没有磷酸化。结果表明wsv083可以催化自身磷酸化。但其具体功能还需进一步研究。
Shrimp white spot syndrome virus (white spot syndrome virus, WSSV) is arod-shaped DNA virus, with the envelop and the tegument, DNA as double chainring. WSSV is currently the main diseases of prawn aquaculture, caused massiveharm. Therefore, the study on properties and infection mechanism of WSSV is ofgreat significance for prawn aquaculture.
The main content of this study are as follows:
①determination of WSSV particle mass and its basic components;
②investigate the role of the microbial infection in the death of crayfishinfected with WSSV;
③the identification of the WSSV particle surface protein;
④a WSSV protein kinase wsv083were studied.
The results are as follows:
First the mass of WSSV genome was calculated, and the proportion of thegenomic DNA accounted for total WSSV particle virus mass was also measured,finally calculate the mass of a complete WSSV particle. The relative proportionsof the major envelop protein were also studied. WSSV suspension for each of theOD600was equivalent to3.34×108/L of the virus concentration.In addition,WSSV components in total weight in proportion through the determination wereas follows: the genomic DNA accounted for10.17%, nucleocapsid protein is43.07%, envelop lipid content is22%, envelop protein content is24.76%.
To study the role of microorganism in the process of death of theProcambarus clarkia infected with WSSV(white spot syndrome virus), themortality rate of Procambarus clarkia injected with antibiotics decreasedsignificantly compared with the control group. Plate count showed that thehemolymph of Procambarus clarkia infected with WSSV have significantly increased the number of microorganisms. Analysis showed that they wereAeromonas hydrophila, Citrobacter freundii, Stenotrophomonas maltophilia andAcinetobacter junii. All of them were opportunistic pathogen. Antibiotic had nosignificant effect on the WSSV’s proliferation.The results showed that thedisappearance of the blood cells of Procambarus clarkia infected with WSSV invivo led to a substantial increase in the number of pathogenic microorganisms,thereby accelerating the rate of Procambarus clarkia deaths.
Using the biotin label transfer technique, we found that the biotin label wastransferred from BSA or gill member protein of Litopenaeus vannamei to theenvelop protein of WSSV. Western blotting showed that two proteins whosemolecular weight among50-55KD were labeled with biotin. It was concludedthat the two proteins was location on the surface of the WSSV viron and couldinteract with the gill member protein of Litopenaeus vannamei. They wereestimated to be VP52A and VP56.
VP28、VP19and wsv083were expressed in E. coli and purified. They wereput in kinase buffer to learn if wsv083can catalyze VP28and VP19’sphosphorylation. Results showed that wsv083cann’t. We also found that therewere threonine and tyrosine phosphorylation but no serine phosphorylation inwsv083. And wsv083mutant was not phosphorylated. It was concluded thatwsv083catalyze autophosphorylation. But its specific function needs furtherstudy.
引文
1、Lightner D V. A handbook of pathology and diagnostic procedures fordiseases of penaeid shrimp [M]. Louisiana, USA; Baton Rouge,1996.
2、Chou H Y, Huang C Y, Wang C H, et al. Pathogenicity of a baculovirusinfection causing white spot syndrome in cultured penaeid shrimp in Taiwan [J].Dis. Aquat. Org.,1995,23:165-173.
3、 Wang C H, Lo C F, Leu J H, et al. Purification and genomic analysis ofbaculovirus associated with spot syndrome (WSBV) of Penaeus monodon [J].Dis. Aquat. Org.,1995,23:239-242.
4、郭银汗，林诗发，杨小强等．福州地区白斑病病毒的超微结构[J].中国病毒学，2000，15:277-284.
5、陈细法，陈平，吴定虎等．养殖对虾一种新杆状病毒的研究[J].中国科学，1997，27:415-420.
6、Inouye K, Miwa S, Oseko N, et al. Mass mortalities of cultured kurumashrimp Penaeus japonicus in Japan in1993: electron microscopic evidence of thecausative virus [J]. Fish Pathol.,1994，29:149-158.
7、Wongteerasupara C, Vickers J E, Sriurairatana S, et al. A non-occludedbaculovirus that occurs in cells of ectoderm and mesodermal origin and causeshigh mortalities in the black tiger prawn Penaeus monodon [J]. Dis. Aquat. Org.,1995,21:69-77.
8、Chou H Y, Huang C Y, Wang C H, et al. Pathogenicity of a baculovirusinfection causing white spot syndrome in cultured penaeid shrimp in Taiwan [J].Dis. Aquat. Org,1995,23:165-173.
9、雷质文，黄捷，史成银等．白斑综合症病毒(WSSV)的宿主调查[J].海洋与湖泊，2002，33:250-258.
10、薛清刚，王文兴．对虾疾病的研究现状与我国虾病泛滥的成因分析[J].海洋科学，1995，1:20-22.
11、Momoyama K, Hiraoka M, Nakano H, et al. Mass mortalities of culturedkuruma shrimp, Penaeus japonicus, in Japan in1993: histopathological study [J].Fish Pathol.,1994,29:141-148.
12、Flegel T W. Major viral diseases of the black tiger prawn (Penaeusmonodon) in Thailand [J]. World J. Microb. Biot.,1997,13:433-442.
13、Kanchanaphum P, Wongteerasupaya C, Sitidilokratana N, et al.Experimental transmission of white spot syndrome virus (WSSV) from crabs toshrimp Penaeus monodon [J]. Dis. Aquat. Organ.,1998,34:1-7.
14、魏静，陆承平，黄捷等．用对虾的致病病毒人工感染克氏原螯虾[J].南京农业大学学报，1998，21:78-82.
15、朱建中，陆承平．对虾白斑综合征病毒在动物模型体内增殖特性研究[J].水产学报，2001，25:47-51.
16、黄灿华，石正丽，张建红，等．原位杂交研究对虾白斑综合症病毒在虾体内感染过程[J].病毒学报，2000，3:242-246.
17、吴友吕，王方国，洪健．长毛对虾杆状病毒的研究[J].电子显微镜学报，1994，13:356.
18、国际翔，王丽霞，李文清等．对虾杆状病毒感染寄主细胞的超微结构观察[J].海洋科学，1994,6:38-42.
19、张立人，张建红，陈隶华等．东方对虾杆状病毒在宿主细胞内的装配[J].电子显微学报，1994,13:354.
20、Durand S. Ultrastructure and morphogeniesis of white spot bacilliformbaculovirus (WSBV)[J]. Dis. Aquat. Org.,1997,29:205-211.
21、周宸，曾志南，陈木．对虾杆状病毒组织病理研究[C].第二捷全国人工养殖对虾疾病综合防治和环境管理学术研讨会论文集,中国科学技术协会学会部编．青岛:青岛海洋大学出版社.1996,15-18.
22、Lo C F, Leu J H, Ho C H, et al. Detection of baculovirus associated withwhite spot syndrome (WSBV) in penaeid shrimps using polymerase chainreaction [J]. Dis. Aquat. Org,1996,25:133-141.
23、吕玲，何建国，邓敏等．核酸探针原位杂交检测白斑综合症病毒的组织特异性[J].热带海洋，2000，19:86-91.
24、Poulos B T, Mari J, Bonami J R, et al. Use of nono-radioactively labeledDNA probes for the detection of a baculovirus from Penaeus monodon(PmSNPV=MBV) by in situ hybridization on fixed tissue [J]. J. Virol. Methods,1994,49:187-193.
25、Wongteerasupays C, Wongwisansri S, Boonsaeng V, et al. DNA fragmentof Penaeus monodon baculovirus PmNOB II gives positive in situ hybridizationwith white-spot viral infections in six penaeid shrimp species [J]. Aquaculture,1996,143:23-32.
26、Yang F, Wang W, Chen R Z, et al. A simple and efficient method forpurification of prawn baculovirus DNA [J]. J. Virol. Methods,1997,67:1-4.
27、Huang C H, Zhang L R, Zhang J H, et al. Purification and characterizationof white spot syndrome virus (WSSV) produced in an alternate host: crayfish,Cambarus clarkii [J]. Virus Res.,2001,76:115-125.
28、van Hulten M C W, Witteveldt J, Peters S, et al. The white spot syndromevirus DNA genome sequence [J]. Virology,2001,286:7-22.
29、Xie, X., Li, H., Xu, L, and Yang, F.2005. A simple and efficient methodfor purification of intact white spot syndrome virus (WSSV) viral particles.Virus Res.108:63-67.
30、黄倢、杨丛海、于佳等．T-E染色法用于对虾爆发性流行病的现场快速诊断[J].海洋科学1995，1：29-33
31、战文斌，王远红，铃木信一等．白斑病病毒在日本对虾体内的感染增殖[J].水产学报，1999,23(3):278-282.
32、黄捷，于佳，王秀华等．单克隆抗体酶联免疫技术检测对虾皮下及造血器官组织坏死病的病原及其传播途径[J].海洋水产研究,1995,16(1):40-50.
33、汪岷，戴继勋，张士璀等．用ELISA检测中国对虾的白斑综合征病毒(WSSV)[J].海洋学报,2000,22(2):132-136.
34、Nunan LM, Lightner D V. Development of a non-radioactive gene probe byPCR for detection of white spot syndrome virus (WSSV)[J]. J. Virol. Methods.,1997,63:193-201.
35、Lo C F, Leu J H, Ho C H, et al. Detection of baculovirus associated whitespot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction [J].Dis. Aquat. Org.,1996,25:133-141.
36、Wang W, He J, Yang F, et al. Detection of prawn white spot baculovirus bypolymerase chain reaction [J]. Acta Oceanologica sinica.,1997,18:591-598.
37、Hsu H C, Lo C F, Lin S C, et al. Studies on effective PCR screeningstrategies for white spot syndrome virus (WSSV) detection in Penaeus monodonbrooders [J]. Dis. Aquat. Org.,1999,39:13-19.
38、Kiatpathomchai W, Boonsaeng V, Tassanakajon A, et al. A non-stop,single-tube, semi-nested PCR technique for grading the severity of white spotsyndrome virus infections in Penaeus monodon [J]. Dis. Aquat. Org.,2001,47:235-239.
39、吕玲，何建国，谢数涛等．白斑综合征杆状病毒(WSBV)PCR检测方法的改进及应用[J].热带海洋,2000,19:90-95.
40、Tsai J M, Shiau L J, Lee H H, et al. Simultaneous detection of white spotsyndrome virus (WSSV) and Taura syndrome virus (TSV) by multiplex reversetranscription-polymerase chain reaction (RT-PCR) in pacific white shrimpPenaeus vannamei [J]. Dis. Aquat. Org.,2002,50:9-12.
41、Tang K F J, Lightner D V. Quantification of white spot syndrome virusDNA through a competitive polymerase chain reaction [J]. Aquaculture,2000,189:11-21.
42、徐丽美，王纬，杨丰．对虾白斑杆状病毒(WSBV)定量PCR检测技术研究[J].高技术通讯,2001,1:14-16.
43、王忠发，邵俊斌，沃健儿等．Taqman实时荧光定量PCR快速检测白斑综合症病毒的方法研究[J].中国卫生检验杂志,2005,15:663-665.
44、徐丽美，杨丰．利用定量PCR方法研究对虾白斑杆状病毒感染与发病的关系[J].高技术通讯,2001,12:9-11.
45、雷质文，史成银，黄倢等． PCR法制备地高辛标记探针斑点杂交检测白斑综合症病毒(WSSV)[J].青岛海洋大学学报，2001,31（2）：201-204
46、朱建中，夏晓勤，陆承平等．斑点杂交检测对虾白斑综合症病毒青岛株在螯虾体内的动态分布[J].中国病毒学2001,16（1）：92-95
47、Lo C F, Heu H C, Tsai M F, et al. Specific genomic DNA fragment analysisof different geographical clinical samples of shrimp white spot syndrome virus[J]. Dis. Aquat. Org.,1999,35:175-185.
48、Yang F, He J, Lin X H, et al. Complete genome sequence of the shrimpwhite spot bacilliform virus [J]. J. Virol.,2001,75:11811-11820.
49、Marks H, Goldbach R W, Vlak J M, et al. Genetic variation among isolatesof White spot syndrome virus [J]. Arch. Virol.,2003,149:673-697.
50、Mayo M A. A summary of taxonomic changes recently approved by ICTV[J]. Arch. Virol.,2002,147:1655-1656.
51、Lan Y, Xu X, Yang F, et al. Transcriptional profile of shrimp white spotsyndrome virus (WSSV) genes with DNA microarray [J]. Arch. Virol.,2006, inpress.
52、Lan Y S, Lu W, Xu X. Genomic instability of prawn white spot bacilliformvirus (WSBV) and its association to virus virulence [J]. Virus Res.,2002,90:269-274.
53、吴常嵩，杨丰．不同年份对虾白斑综合征病毒基因组差异的微阵列分析[J].高技术通讯，2006，16:210-203.
54、Tsai J. M., Wang H. C., Leu J. H., et al. Identification of the nucleocapsid,tegument, and envelope proteins of the shrimp white spot syndrome virusvirion[J]. Journal of virology,2006,80(6):3021-3029.
55、van Hulten M C W, Westenberg M, Goodall S D, et al. Identification of twomajor virion protein genes of white spot syndrome virus of shrimp [J]. Virology,2000,266:227-236.
56、van Hulten M C W, Reijns M, Vermeesch A M G, et al. Identification ofVP19and VP15of white spot syndrome virus (WSSV) and glycosylation statusof the WSSV major structural proteins [J]. J. Gen. Virol.,200283:257-265.
57、Xie X, Xu L, Yang F. Proteomic analysis of the major envelope andnucleocapsid proteins of white spot syndrome virus (WSSV)[J].Journal ofVirology,2006,80(21):10615-10623
58、van Hulten M. C., Witteveldt J., Snippe M., et al. White spot syndromevirus envelope protein VP28is involved in the systemic infection of shrimp[J].Virology,2001,285(2):228-233.
59、Witteveldt J., Cifuentes C. C., Vlak J. M., et al. Protection of Penaeusmonodon against white spot syndrome virus by oral vaccination[J]. Journal ofvirology,2004,78(4):2057-2061.
60、Yi G., Wang Z., Qi Y., et al. Vp28of shrimp white spot syndrome virus isinvolved in the attachment and penetration into shrimp cells[J]. Journal ofbiochemistry and molecular biology,2004,37(6):726-734.
61、van Hulten M. C., Goldbach R. W., Vlak J. M. Three functionally divergedmajor structural proteins of white spot syndrome virus evolved by geneduplication[J]. The Journal of general virology,2000,81(Pt10):2525-2529.
62、van Hulten M. C., Reijns M., Vermeesch A. M., et al. Identification ofVP19and VP15of white spot syndrome virus (WSSV) and glycosylation statusof the WSSV major structural proteins[J]. The Journal of general virology,2002,83(Pt1):257-265.
63、Zhang X., Huang C., Xu X., et al. Transcription and identification of anenvelope protein gene (p22) from shrimp white spot syndrome virus[J]. TheJournal of general virology,2002,83(Pt2):471-477.
64、Xie, X., and Yang, F.2005. Interaction of white spot syndrome virus VP26protein with actin. Virology336:93-99.
65、Qiang Wan, Limei Xu, Feng Yang.VP26of White Spot Syndrome VirusFunctions as a Linker Protein between the Envelope and Nucleocapsid ofVirions by Binding with VP51.[J]. Journal of Virology,2008,82(24):12598–12601
66、Chang Y. S., Liu W. J., Chou T. L., et al. Characterization of white spotsyndrome virus envelope protein VP51A and its interaction with viral tegumentprotein VP26[J]. Journal of virology,2008,82(24):12555-12564.
67、van Hulten M C W, Goldbach R W, Vlak J M. Three functionally divergedmajor structural proteins of white spot syndrome virus evolved by geneduplication [J]. J. Gen. Virol.,2000,81:2525-2529.
68、Xie, X., and Yang, F.2006. White spot syndrome virus VP24interacts withVP28and is involved in virus infection. J. Gen. Virol.87:1903-1908
69、Chen J., Li Z., Hew C. L. Characterization of a novel envelope proteinWSV010of shrimp whitespot syndrome virus and its interaction with a majorviral structural protein VP24[J]. Virology,2007,364(1):208-213.
70、Jie Z., Xu L., Yang F. The C-terminal region of envelope protein VP38from white spot syndrome virus is indispensable for interaction with VP24[J].Archives of virology,2008,153(11):2103-2106.
71、Shih H H. Neutralization of white spot syndrome virus by monoclonalantibodies against viral envelope proteins [J]. Taiwania,2004,49:159-165.
72、刘庆慧，黄倢，贾佩峤等．WSSV具蛋白酶活性肽段的检测[J].中国水产科学2001,11（6）：531-536
73、Zhang X, Xu X, Hew C L. The structure and function of a gene encoding abasic peptide from prawn white spot syndrome virus [J]. Virus Res.2001,79:137-144.
74、Chen L L, Leu J H, Huang C J, et al. Identification of a nucleocapsidprotein (VP35) gene of shrimp white spot syndrome virus and characterization ofthe motif important for targeting VP35to the nuclei of transfected insect cells [J].Virology,2002,293:44-53.
75、Leu J H, Tsai J M, Wang H C., et al. The unique stacked rings in thenucleocapsid of the white spot syndrome virus virion are formed by the majorstructural protein VP664, the largest viral structural protein ever found [J]. JVirol.,2005,79:140-149.
76、Tsai J. M., Wang H. C., Leu J. H., et al. Genomic and proteomic analysis ofthirty-nine structural proteins of shrimp white spot syndrome virus[J]. Journal ofvirology,2004,78(20):11360-11370.
77、Li L, Lin S, Yang F. Characterization of an envelope protein (VP110) ofwhite spot syndrome virus [J]. J. Gen. Virol.,2006,87:1909-1915.
78、Tsai J. M., Wang H. C., Leu J. H., et al. Genomic and proteomic analysis ofthirty-nine structural proteins of shrimp white spot syndrome virus[J]. Journal ofvirology,2004,78(20):11360-11370.
79、Huang C., Zhang L., Zhang J., et al. Purification and characterization ofWhite Spot syndrome virus (WSSV) produced in an alternate host: crayfish,Cambarus clarkii[J]. Virus research,2001,76(2):115-125.
80、Tsai J. M., Wang H. C., Leu J. H., et al. Identification of the nucleocapsid,tegument, and envelope proteins of the shrimp white spot syndrome virusvirion[J]. Journal of virology,2006,80(6):3021-3029.
81、Lan Y., Xu X., Yang F., et al. Transcriptional profile of shrimp white spotsyndrome virus (WSSV)genes with DNA microarray[J]. Archives of virology,2006,151(9):1723-1733.
82、Huang C., Zhang X., Lin Q., et al. Proteomic analysis of shrimp white spotsyndrome viral proteins and characterization of a novel envelope proteinVP466[J]. Mol Cell Proteomics,2002,1(3):223-231.
83、Tsai J. M., Wang H. C., Leu J. H., et al. Identification of the nucleocapsid,tegument, and envelopeproteins of the shrimp white spot syndrome virusvirion[J]. Journal of virology,2006,80(6):3021-3029.
84、Li H., Zhu Y., Xie X., et al. Identification of a novel envelope protein(VP187) gene fromshrimp white spot syndrome virus[J]. Virus research,2006,115(1):76-84.
85、Li Z., Lin Q., Chen J., et al. Shotgun identification of the structuralproteome of shrimp white spot syndrome virus and iTRAQ differentiation
86、Tsai J. M., Wang H. C., Leu J. H., et al. Genomic and proteomic analysis ofthirty-nine structural proteins of shrimp white spot syndrome virus[J]. Journal ofvirology,2004,78(20):11360-11370.
87、Li L., Lin S., Yang F. Characterization of an envelope protein (VP110) ofWhite spotsyndrome virus[J]. The Journal of general virology,2006,87(Pt7):1909-1915.
88、Li L, Xie X, Yang F. Identification and characterization of a prawn whitespot syndrome virus gene that encodes an envelope protein VP31[J].Virology,2005,340(1):125-132.
89、Li L. J., Yuan J. F., Cai C. A., et al. Multiple envelope proteins are involvedin white spot syndrome virus (WSSV) infection in crayfish[J]. Archives ofvirology,2006,151(7):1309-1317.
90、Huang C., Zhang X., Lin Q., et al. Characterization of a novel envelopeprotein (VP281) of shrimp white spot syndrome virus by mass spectrometry[J].The Journal of general virology,2002,83(Pt10):2385-2392.
91、Wu W., Wang L., Zhang X. Identification of white spot syndrome virus(WSSV) envelope proteins involved in shrimp infection[J]. Virology,2005,332(2):578-583.
92、Liang Y., Huang J., Song X. L., et al. Four viral proteins of white spotsyndrome virus (WSSV) that attach to shrimp cell membranes[J]. Diseases ofaquatic organisms,2005,66(1):81-85.
93、Li Q, Chen Y, Yang F. Identification of a collagen-like protein gene fromwhite spot syndrome virus [J]. Arch. Virol.,2004,149:215-223.
94、Tsai M F, Lo C F, van Hulten M C W, et al. Transcriptional analysis of theribonucleotide reductase genes of shrimp white spot syndrome virus [J].Virology,2000,277:92-99.
95、van Hulten M C W, Tsai M F, Schipper C A, et al. Analysis of a genomicsegment of white spot syndrome virus of shrimp containing ribonucleotidereductase genes and repeat regions [J]. J. Gen. Virol.,2000,81:307-316.
96、Tsai M F, Yu H T, Tzeng H F, et al. Identification and characterization of ashrimp white spot syndrome virus (WSSV) gene that encodes a novel chimericpolypeptide of cellular-type thymidine kinase and thymidylate kinase [J].Virology,2000,277:100-110.
97、Tzeng H F, Chang Z F, Peng S E, et al. Chimeric polypeptide of thymidinekinase and thymidylate kinase of shrimp white spot syndrome virus: thymidinekinase activity of the recombinant protein expressed in a baculovirus/insect cellsystem [J]. Virology,2002,299:248-255.
98、Chen L L, Wang H C, Huang C J, et al. Transcriptional analysis of the DNApolymerase gene of shrimp white spot syndrome virus [J]. Virology,2002,301:136-147.
99、Witteveldt J, van Hulten M C W, Vlak J M. Identification and phylogeny ofa non-specific endonuclease gene of white spot syndrome virus of shrimp [J].Virus Genes,2001,23:331-337.
100、Li L, Lin S, Yang F. Functional identification of the non-specific nucleasefrom white spot syndrome virus [J]. Virology,2005,337:399-406.
101、van Hulten M C W, Vlak J M. Identification and phylogeny of a proteinkinase gene of white spot syndrome virus [J]. Virus Genes,2001,22:201-207.
102、Liu W J, Yu H T, Peng S E, et al. Cloning, characterization, andphylogenetic analysis of a shrimp white spot syndrome virus gene that encodes aprotein kinase [J]. Virology,2001,289:362-377.
103、Li F，Li M，Ke W，Ji Y，Bian X, et al. Identification of the irnmediate-early genes of white spot syndrome virus.Virology2009,385(1):267-274.
104、Li Q, Pan D, Zhang J H, et al.. Identification of the thymidylate synthasewithin the genome of white spot syndrome virus [J]. J. Gen. Virol.,2004,85:2035-2044.
105、丁琦，徐丽美，杨丰．对虾白斑综合征病毒非结构蛋白ICP11的研究[J].厦门大学学报：自然科学版,2009,6:879-883.
106、Liu Y., Wu J., Song J., et al. Identification of a novel nonstructural protein,VP9, from white spot syndrome virus: its structure reveals a ferredoxin fold withspecific metal binding sites[J]. Journal of virology,2006,80(21):10419-10427.
107、Wang H. C., Wang H. C., Kou G. H., et al. Identification of icp11, themost highly expressed gene of shrimp white spot syndrome virus (WSSV)[J].Diseases of aquatic organisms,2007,74(3):179-189.
108、Wang H. C., Wang H. C., Ko T. P., et al. White spot syndrome virusprotein ICP11: A histone-binding DNA mimic that disrupts nucleosomeassembly[J]. Proceedings of the National Academy of Sciences of the UnitedStates of America,2008,105(52):20758-20763.
109、Granja C B, Aranguren L F, Vidal O M, Aragón L,Salazar M. Doeshyperthermia increase apoptosis in white spot syndrome virus (WSSV) infectedLitopenaeus vannamei?[J] Dis Aquat Org,2003,54:73—78
110、冯守明，杨先乐，李军等．凡纳滨对虾白斑综合征血液病理研究[J].水产学报2006，30(1):108-112
111、李继秋，谭北平，麦康森．白斑综合征病毒与凡纳滨对虾肠道菌群区系之间关系的初步研究[J].上海水产大学学报，2006，15(1):109-113
112、陈细法，吴定虎，黄槐等．对虾病毒病病毒与细菌合并感染的病理特点和诊断价值[J].病毒学报1997，13(2):146～150.
113、周勤，郭光远．弗劳地枸橼酸杆菌引起的猕猴腹泻[J].上海实验动物科学2004，24(1):39-40
114、陈翠珍，张晓君，房海等．中华绒螯蟹病原弗氏柠檬酸杆菌的鉴定[J].中国人兽共患病杂志2006，22(2):136-141
115、李华，邢殿楼，白国福等．弗氏柠檬酸杆菌对河蟹致病性的研究[J].水生生物学报2005，25（3）：217-223
116、冯迎春，颜其贵．从山羊鼻内腺瘤病例中分离出嗜麦芽窄食单胞菌的报告[J].畜牧与兽医2009，41（12）：81-83
117、耿毅，汪开毓，陈德芳等.斑点叉尾鮰一株致病菌的分离、鉴定与系统发育分析[J].微生物学报，2006，46(4):28-31
118、耿毅，汪开毓，黄小丽等．一种斑点叉尾鮰急性暴发性细菌性传染病初报[J].科学养鱼，2005，(3):51-52
119、周永灿，朱伟华．卵形鲳大规模个体病原及其防治[J].海洋科学，2001，(4):40-44.
120、黄斌，陈世峰．黄缘闭壳龟囊肿病的研究[J].淡水渔业，2002，(5):44-46
121、Harris N B, Rogers D G. Septicemia associated with Stenotrophomonasmaltophilia in a West African dwarf crocodile (Osteolaemus tetraspis subsp.tetraspis)[J]. Journal of Veterinary Diagnostic Investigation,2001,13(3):255-258
122、陈翠珍，房海，张晓君．石鲽病原琼氏不动杆菌形态型Ⅰ的鉴定[J].微生物学通报，2005，32(3)：34-39
123、陈翠珍，张晓君，房海等．锦鲤中琼氏不动杆菌的分离与鉴定[J].水生态学杂志，2009，2（1）：86-90
124、王祥红，李会荣，张晓华等.中国对虾成虾肠道微生物区系[J].青岛海洋大学学报，2000，30(3):493～498
125、Aoki T.Motile Aeromonads(AeromonashydroPhila).In CABIntemationa]，Fish Disease and disorders:Volume3;viral，baeterial and Fungalinfeetions (eds.Woo PTK and Bruno DW)，1999，427-453
126、Zhang X B, Huang C, Xu X, et al. Transcription and identification of anenvelope protein gene (p22)from shrimp white spot syndrome virus[J]. TheJournal of general virology,2002,83(2):471-477.
127、Huang C, Zhang X, Lin Q, et al. Proteomic analysis of shrimp white spotsyndrome viral proteins and characterization of a novel envelope proteinVP466[J].Mol Cell Proteomics,2002,1(3):223-231.
128、Faoud T Ishmael, Michael A Trakselis,Stephen J Benkovic.Protein-Protein Interactions in the Bacteriophage T4Replisome[J].The Journalof Biological Chemistry,2003,278(5):3145-3152.
129、Linda D Kosturko, Michael J Maggipinto, George Korza, et al.Heterogeneous Nuclear Ribonucleoprotein (hnRNP) E1Binds to hnRNP A2andInhibits Translation of A2Response Element mRNAs[J].Molecular Biology ofthe Cell,2006,17:3521-3533.
130、Yves Laumonnier, Tatiana Syrovets, Ladislva Burysek,et al. Identificationof the annexin A2heterotetramer as a receptor for the plasmin-induced signalingin human peripheral monocytes[J].Blood,2006,107:3342-3349.
131、Kristen E Neely, Ahmed H Hassan, Christine E Brown, et al. TranscriptionActivator Interactions with Multiple SWI/SNF Subunits[J].Molecular andCellular Biology,2002,22(6):1615-1625.
132、Pierre-Olivier Mari, Bogdan I Florea, Strphan P Persengiev,et al. Dynamicassembly of end-joining complexes requires interaction between Ku70/80andXRCC4[J].Proceedings of the National Academy of Sciences of the UnitedStates of America,2006,106:18597-18602.
133、梁艳，黄倢，易志刚等．对虾组织膜蛋白制备方法及其多肽组成[J].中国水产科学,2005,12(3):348-351.
134、Zhang X B, Huang C, Xu X, et al. Identification and localization of aprawn white spot syndrome virus gene that encodes an envelope protein[J].Journal of General Virology,2002,83:1069-1074.
2、Chou H Y, Huang C Y, Wang C H, et al. Pathogenicity of a baculovirusinfection causing white spot syndrome in cultured penaeid shrimp in Taiwan [J].Dis. Aquat. Org.,1995,23:165-173.
3、 Wang C H, Lo C F, Leu J H, et al. Purification and genomic analysis ofbaculovirus associated with spot syndrome (WSBV) of Penaeus monodon [J].Dis. Aquat. Org.,1995,23:239-242.
4、郭银汗，林诗发，杨小强等．福州地区白斑病病毒的超微结构[J].中国病毒学，2000，15:277-284.
5、陈细法，陈平，吴定虎等．养殖对虾一种新杆状病毒的研究[J].中国科学，1997，27:415-420.
6、Inouye K, Miwa S, Oseko N, et al. Mass mortalities of cultured kurumashrimp Penaeus japonicus in Japan in1993: electron microscopic evidence of thecausative virus [J]. Fish Pathol.,1994，29:149-158.
7、Wongteerasupara C, Vickers J E, Sriurairatana S, et al. A non-occludedbaculovirus that occurs in cells of ectoderm and mesodermal origin and causeshigh mortalities in the black tiger prawn Penaeus monodon [J]. Dis. Aquat. Org.,1995,21:69-77.
8、Chou H Y, Huang C Y, Wang C H, et al. Pathogenicity of a baculovirusinfection causing white spot syndrome in cultured penaeid shrimp in Taiwan [J].Dis. Aquat. Org,1995,23:165-173.
9、雷质文，黄捷，史成银等．白斑综合症病毒(WSSV)的宿主调查[J].海洋与湖泊，2002，33:250-258.
10、薛清刚，王文兴．对虾疾病的研究现状与我国虾病泛滥的成因分析[J].海洋科学，1995，1:20-22.
11、Momoyama K, Hiraoka M, Nakano H, et al. Mass mortalities of culturedkuruma shrimp, Penaeus japonicus, in Japan in1993: histopathological study [J].Fish Pathol.,1994,29:141-148.
12、Flegel T W. Major viral diseases of the black tiger prawn (Penaeusmonodon) in Thailand [J]. World J. Microb. Biot.,1997,13:433-442.
13、Kanchanaphum P, Wongteerasupaya C, Sitidilokratana N, et al.Experimental transmission of white spot syndrome virus (WSSV) from crabs toshrimp Penaeus monodon [J]. Dis. Aquat. Organ.,1998,34:1-7.
14、魏静，陆承平，黄捷等．用对虾的致病病毒人工感染克氏原螯虾[J].南京农业大学学报，1998，21:78-82.
15、朱建中，陆承平．对虾白斑综合征病毒在动物模型体内增殖特性研究[J].水产学报，2001，25:47-51.
16、黄灿华，石正丽，张建红，等．原位杂交研究对虾白斑综合症病毒在虾体内感染过程[J].病毒学报，2000，3:242-246.
17、吴友吕，王方国，洪健．长毛对虾杆状病毒的研究[J].电子显微镜学报，1994，13:356.
18、国际翔，王丽霞，李文清等．对虾杆状病毒感染寄主细胞的超微结构观察[J].海洋科学，1994,6:38-42.
19、张立人，张建红，陈隶华等．东方对虾杆状病毒在宿主细胞内的装配[J].电子显微学报，1994,13:354.
20、Durand S. Ultrastructure and morphogeniesis of white spot bacilliformbaculovirus (WSBV)[J]. Dis. Aquat. Org.,1997,29:205-211.
21、周宸，曾志南，陈木．对虾杆状病毒组织病理研究[C].第二捷全国人工养殖对虾疾病综合防治和环境管理学术研讨会论文集,中国科学技术协会学会部编．青岛:青岛海洋大学出版社.1996,15-18.
22、Lo C F, Leu J H, Ho C H, et al. Detection of baculovirus associated withwhite spot syndrome (WSBV) in penaeid shrimps using polymerase chainreaction [J]. Dis. Aquat. Org,1996,25:133-141.
23、吕玲，何建国，邓敏等．核酸探针原位杂交检测白斑综合症病毒的组织特异性[J].热带海洋，2000，19:86-91.
24、Poulos B T, Mari J, Bonami J R, et al. Use of nono-radioactively labeledDNA probes for the detection of a baculovirus from Penaeus monodon(PmSNPV=MBV) by in situ hybridization on fixed tissue [J]. J. Virol. Methods,1994,49:187-193.
25、Wongteerasupays C, Wongwisansri S, Boonsaeng V, et al. DNA fragmentof Penaeus monodon baculovirus PmNOB II gives positive in situ hybridizationwith white-spot viral infections in six penaeid shrimp species [J]. Aquaculture,1996,143:23-32.
26、Yang F, Wang W, Chen R Z, et al. A simple and efficient method forpurification of prawn baculovirus DNA [J]. J. Virol. Methods,1997,67:1-4.
27、Huang C H, Zhang L R, Zhang J H, et al. Purification and characterizationof white spot syndrome virus (WSSV) produced in an alternate host: crayfish,Cambarus clarkii [J]. Virus Res.,2001,76:115-125.
28、van Hulten M C W, Witteveldt J, Peters S, et al. The white spot syndromevirus DNA genome sequence [J]. Virology,2001,286:7-22.
29、Xie, X., Li, H., Xu, L, and Yang, F.2005. A simple and efficient methodfor purification of intact white spot syndrome virus (WSSV) viral particles.Virus Res.108:63-67.
30、黄倢、杨丛海、于佳等．T-E染色法用于对虾爆发性流行病的现场快速诊断[J].海洋科学1995，1：29-33
31、战文斌，王远红，铃木信一等．白斑病病毒在日本对虾体内的感染增殖[J].水产学报，1999,23(3):278-282.
32、黄捷，于佳，王秀华等．单克隆抗体酶联免疫技术检测对虾皮下及造血器官组织坏死病的病原及其传播途径[J].海洋水产研究,1995,16(1):40-50.
33、汪岷，戴继勋，张士璀等．用ELISA检测中国对虾的白斑综合征病毒(WSSV)[J].海洋学报,2000,22(2):132-136.
34、Nunan LM, Lightner D V. Development of a non-radioactive gene probe byPCR for detection of white spot syndrome virus (WSSV)[J]. J. Virol. Methods.,1997,63:193-201.
35、Lo C F, Leu J H, Ho C H, et al. Detection of baculovirus associated whitespot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction [J].Dis. Aquat. Org.,1996,25:133-141.
36、Wang W, He J, Yang F, et al. Detection of prawn white spot baculovirus bypolymerase chain reaction [J]. Acta Oceanologica sinica.,1997,18:591-598.
37、Hsu H C, Lo C F, Lin S C, et al. Studies on effective PCR screeningstrategies for white spot syndrome virus (WSSV) detection in Penaeus monodonbrooders [J]. Dis. Aquat. Org.,1999,39:13-19.
38、Kiatpathomchai W, Boonsaeng V, Tassanakajon A, et al. A non-stop,single-tube, semi-nested PCR technique for grading the severity of white spotsyndrome virus infections in Penaeus monodon [J]. Dis. Aquat. Org.,2001,47:235-239.
39、吕玲，何建国，谢数涛等．白斑综合征杆状病毒(WSBV)PCR检测方法的改进及应用[J].热带海洋,2000,19:90-95.
40、Tsai J M, Shiau L J, Lee H H, et al. Simultaneous detection of white spotsyndrome virus (WSSV) and Taura syndrome virus (TSV) by multiplex reversetranscription-polymerase chain reaction (RT-PCR) in pacific white shrimpPenaeus vannamei [J]. Dis. Aquat. Org.,2002,50:9-12.
41、Tang K F J, Lightner D V. Quantification of white spot syndrome virusDNA through a competitive polymerase chain reaction [J]. Aquaculture,2000,189:11-21.
42、徐丽美，王纬，杨丰．对虾白斑杆状病毒(WSBV)定量PCR检测技术研究[J].高技术通讯,2001,1:14-16.
43、王忠发，邵俊斌，沃健儿等．Taqman实时荧光定量PCR快速检测白斑综合症病毒的方法研究[J].中国卫生检验杂志,2005,15:663-665.
44、徐丽美，杨丰．利用定量PCR方法研究对虾白斑杆状病毒感染与发病的关系[J].高技术通讯,2001,12:9-11.
45、雷质文，史成银，黄倢等． PCR法制备地高辛标记探针斑点杂交检测白斑综合症病毒(WSSV)[J].青岛海洋大学学报，2001,31（2）：201-204
46、朱建中，夏晓勤，陆承平等．斑点杂交检测对虾白斑综合症病毒青岛株在螯虾体内的动态分布[J].中国病毒学2001,16（1）：92-95
47、Lo C F, Heu H C, Tsai M F, et al. Specific genomic DNA fragment analysisof different geographical clinical samples of shrimp white spot syndrome virus[J]. Dis. Aquat. Org.,1999,35:175-185.
48、Yang F, He J, Lin X H, et al. Complete genome sequence of the shrimpwhite spot bacilliform virus [J]. J. Virol.,2001,75:11811-11820.
49、Marks H, Goldbach R W, Vlak J M, et al. Genetic variation among isolatesof White spot syndrome virus [J]. Arch. Virol.,2003,149:673-697.
50、Mayo M A. A summary of taxonomic changes recently approved by ICTV[J]. Arch. Virol.,2002,147:1655-1656.
51、Lan Y, Xu X, Yang F, et al. Transcriptional profile of shrimp white spotsyndrome virus (WSSV) genes with DNA microarray [J]. Arch. Virol.,2006, inpress.
52、Lan Y S, Lu W, Xu X. Genomic instability of prawn white spot bacilliformvirus (WSBV) and its association to virus virulence [J]. Virus Res.,2002,90:269-274.
53、吴常嵩，杨丰．不同年份对虾白斑综合征病毒基因组差异的微阵列分析[J].高技术通讯，2006，16:210-203.
54、Tsai J. M., Wang H. C., Leu J. H., et al. Identification of the nucleocapsid,tegument, and envelope proteins of the shrimp white spot syndrome virusvirion[J]. Journal of virology,2006,80(6):3021-3029.
55、van Hulten M C W, Westenberg M, Goodall S D, et al. Identification of twomajor virion protein genes of white spot syndrome virus of shrimp [J]. Virology,2000,266:227-236.
56、van Hulten M C W, Reijns M, Vermeesch A M G, et al. Identification ofVP19and VP15of white spot syndrome virus (WSSV) and glycosylation statusof the WSSV major structural proteins [J]. J. Gen. Virol.,200283:257-265.
57、Xie X, Xu L, Yang F. Proteomic analysis of the major envelope andnucleocapsid proteins of white spot syndrome virus (WSSV)[J].Journal ofVirology,2006,80(21):10615-10623
58、van Hulten M. C., Witteveldt J., Snippe M., et al. White spot syndromevirus envelope protein VP28is involved in the systemic infection of shrimp[J].Virology,2001,285(2):228-233.
59、Witteveldt J., Cifuentes C. C., Vlak J. M., et al. Protection of Penaeusmonodon against white spot syndrome virus by oral vaccination[J]. Journal ofvirology,2004,78(4):2057-2061.
60、Yi G., Wang Z., Qi Y., et al. Vp28of shrimp white spot syndrome virus isinvolved in the attachment and penetration into shrimp cells[J]. Journal ofbiochemistry and molecular biology,2004,37(6):726-734.
61、van Hulten M. C., Goldbach R. W., Vlak J. M. Three functionally divergedmajor structural proteins of white spot syndrome virus evolved by geneduplication[J]. The Journal of general virology,2000,81(Pt10):2525-2529.
62、van Hulten M. C., Reijns M., Vermeesch A. M., et al. Identification ofVP19and VP15of white spot syndrome virus (WSSV) and glycosylation statusof the WSSV major structural proteins[J]. The Journal of general virology,2002,83(Pt1):257-265.
63、Zhang X., Huang C., Xu X., et al. Transcription and identification of anenvelope protein gene (p22) from shrimp white spot syndrome virus[J]. TheJournal of general virology,2002,83(Pt2):471-477.
64、Xie, X., and Yang, F.2005. Interaction of white spot syndrome virus VP26protein with actin. Virology336:93-99.
65、Qiang Wan, Limei Xu, Feng Yang.VP26of White Spot Syndrome VirusFunctions as a Linker Protein between the Envelope and Nucleocapsid ofVirions by Binding with VP51.[J]. Journal of Virology,2008,82(24):12598–12601
66、Chang Y. S., Liu W. J., Chou T. L., et al. Characterization of white spotsyndrome virus envelope protein VP51A and its interaction with viral tegumentprotein VP26[J]. Journal of virology,2008,82(24):12555-12564.
67、van Hulten M C W, Goldbach R W, Vlak J M. Three functionally divergedmajor structural proteins of white spot syndrome virus evolved by geneduplication [J]. J. Gen. Virol.,2000,81:2525-2529.
68、Xie, X., and Yang, F.2006. White spot syndrome virus VP24interacts withVP28and is involved in virus infection. J. Gen. Virol.87:1903-1908
69、Chen J., Li Z., Hew C. L. Characterization of a novel envelope proteinWSV010of shrimp whitespot syndrome virus and its interaction with a majorviral structural protein VP24[J]. Virology,2007,364(1):208-213.
70、Jie Z., Xu L., Yang F. The C-terminal region of envelope protein VP38from white spot syndrome virus is indispensable for interaction with VP24[J].Archives of virology,2008,153(11):2103-2106.
71、Shih H H. Neutralization of white spot syndrome virus by monoclonalantibodies against viral envelope proteins [J]. Taiwania,2004,49:159-165.
72、刘庆慧，黄倢，贾佩峤等．WSSV具蛋白酶活性肽段的检测[J].中国水产科学2001,11（6）：531-536
73、Zhang X, Xu X, Hew C L. The structure and function of a gene encoding abasic peptide from prawn white spot syndrome virus [J]. Virus Res.2001,79:137-144.
74、Chen L L, Leu J H, Huang C J, et al. Identification of a nucleocapsidprotein (VP35) gene of shrimp white spot syndrome virus and characterization ofthe motif important for targeting VP35to the nuclei of transfected insect cells [J].Virology,2002,293:44-53.
75、Leu J H, Tsai J M, Wang H C., et al. The unique stacked rings in thenucleocapsid of the white spot syndrome virus virion are formed by the majorstructural protein VP664, the largest viral structural protein ever found [J]. JVirol.,2005,79:140-149.
76、Tsai J. M., Wang H. C., Leu J. H., et al. Genomic and proteomic analysis ofthirty-nine structural proteins of shrimp white spot syndrome virus[J]. Journal ofvirology,2004,78(20):11360-11370.
77、Li L, Lin S, Yang F. Characterization of an envelope protein (VP110) ofwhite spot syndrome virus [J]. J. Gen. Virol.,2006,87:1909-1915.
78、Tsai J. M., Wang H. C., Leu J. H., et al. Genomic and proteomic analysis ofthirty-nine structural proteins of shrimp white spot syndrome virus[J]. Journal ofvirology,2004,78(20):11360-11370.
79、Huang C., Zhang L., Zhang J., et al. Purification and characterization ofWhite Spot syndrome virus (WSSV) produced in an alternate host: crayfish,Cambarus clarkii[J]. Virus research,2001,76(2):115-125.
80、Tsai J. M., Wang H. C., Leu J. H., et al. Identification of the nucleocapsid,tegument, and envelope proteins of the shrimp white spot syndrome virusvirion[J]. Journal of virology,2006,80(6):3021-3029.
81、Lan Y., Xu X., Yang F., et al. Transcriptional profile of shrimp white spotsyndrome virus (WSSV)genes with DNA microarray[J]. Archives of virology,2006,151(9):1723-1733.
82、Huang C., Zhang X., Lin Q., et al. Proteomic analysis of shrimp white spotsyndrome viral proteins and characterization of a novel envelope proteinVP466[J]. Mol Cell Proteomics,2002,1(3):223-231.
83、Tsai J. M., Wang H. C., Leu J. H., et al. Identification of the nucleocapsid,tegument, and envelopeproteins of the shrimp white spot syndrome virusvirion[J]. Journal of virology,2006,80(6):3021-3029.
84、Li H., Zhu Y., Xie X., et al. Identification of a novel envelope protein(VP187) gene fromshrimp white spot syndrome virus[J]. Virus research,2006,115(1):76-84.
85、Li Z., Lin Q., Chen J., et al. Shotgun identification of the structuralproteome of shrimp white spot syndrome virus and iTRAQ differentiation
86、Tsai J. M., Wang H. C., Leu J. H., et al. Genomic and proteomic analysis ofthirty-nine structural proteins of shrimp white spot syndrome virus[J]. Journal ofvirology,2004,78(20):11360-11370.
87、Li L., Lin S., Yang F. Characterization of an envelope protein (VP110) ofWhite spotsyndrome virus[J]. The Journal of general virology,2006,87(Pt7):1909-1915.
88、Li L, Xie X, Yang F. Identification and characterization of a prawn whitespot syndrome virus gene that encodes an envelope protein VP31[J].Virology,2005,340(1):125-132.
89、Li L. J., Yuan J. F., Cai C. A., et al. Multiple envelope proteins are involvedin white spot syndrome virus (WSSV) infection in crayfish[J]. Archives ofvirology,2006,151(7):1309-1317.
90、Huang C., Zhang X., Lin Q., et al. Characterization of a novel envelopeprotein (VP281) of shrimp white spot syndrome virus by mass spectrometry[J].The Journal of general virology,2002,83(Pt10):2385-2392.
91、Wu W., Wang L., Zhang X. Identification of white spot syndrome virus(WSSV) envelope proteins involved in shrimp infection[J]. Virology,2005,332(2):578-583.
92、Liang Y., Huang J., Song X. L., et al. Four viral proteins of white spotsyndrome virus (WSSV) that attach to shrimp cell membranes[J]. Diseases ofaquatic organisms,2005,66(1):81-85.
93、Li Q, Chen Y, Yang F. Identification of a collagen-like protein gene fromwhite spot syndrome virus [J]. Arch. Virol.,2004,149:215-223.
94、Tsai M F, Lo C F, van Hulten M C W, et al. Transcriptional analysis of theribonucleotide reductase genes of shrimp white spot syndrome virus [J].Virology,2000,277:92-99.
95、van Hulten M C W, Tsai M F, Schipper C A, et al. Analysis of a genomicsegment of white spot syndrome virus of shrimp containing ribonucleotidereductase genes and repeat regions [J]. J. Gen. Virol.,2000,81:307-316.
96、Tsai M F, Yu H T, Tzeng H F, et al. Identification and characterization of ashrimp white spot syndrome virus (WSSV) gene that encodes a novel chimericpolypeptide of cellular-type thymidine kinase and thymidylate kinase [J].Virology,2000,277:100-110.
97、Tzeng H F, Chang Z F, Peng S E, et al. Chimeric polypeptide of thymidinekinase and thymidylate kinase of shrimp white spot syndrome virus: thymidinekinase activity of the recombinant protein expressed in a baculovirus/insect cellsystem [J]. Virology,2002,299:248-255.
98、Chen L L, Wang H C, Huang C J, et al. Transcriptional analysis of the DNApolymerase gene of shrimp white spot syndrome virus [J]. Virology,2002,301:136-147.
99、Witteveldt J, van Hulten M C W, Vlak J M. Identification and phylogeny ofa non-specific endonuclease gene of white spot syndrome virus of shrimp [J].Virus Genes,2001,23:331-337.
100、Li L, Lin S, Yang F. Functional identification of the non-specific nucleasefrom white spot syndrome virus [J]. Virology,2005,337:399-406.
101、van Hulten M C W, Vlak J M. Identification and phylogeny of a proteinkinase gene of white spot syndrome virus [J]. Virus Genes,2001,22:201-207.
102、Liu W J, Yu H T, Peng S E, et al. Cloning, characterization, andphylogenetic analysis of a shrimp white spot syndrome virus gene that encodes aprotein kinase [J]. Virology,2001,289:362-377.
103、Li F，Li M，Ke W，Ji Y，Bian X, et al. Identification of the irnmediate-early genes of white spot syndrome virus.Virology2009,385(1):267-274.
104、Li Q, Pan D, Zhang J H, et al.. Identification of the thymidylate synthasewithin the genome of white spot syndrome virus [J]. J. Gen. Virol.,2004,85:2035-2044.
105、丁琦，徐丽美，杨丰．对虾白斑综合征病毒非结构蛋白ICP11的研究[J].厦门大学学报：自然科学版,2009,6:879-883.
106、Liu Y., Wu J., Song J., et al. Identification of a novel nonstructural protein,VP9, from white spot syndrome virus: its structure reveals a ferredoxin fold withspecific metal binding sites[J]. Journal of virology,2006,80(21):10419-10427.
107、Wang H. C., Wang H. C., Kou G. H., et al. Identification of icp11, themost highly expressed gene of shrimp white spot syndrome virus (WSSV)[J].Diseases of aquatic organisms,2007,74(3):179-189.
108、Wang H. C., Wang H. C., Ko T. P., et al. White spot syndrome virusprotein ICP11: A histone-binding DNA mimic that disrupts nucleosomeassembly[J]. Proceedings of the National Academy of Sciences of the UnitedStates of America,2008,105(52):20758-20763.
109、Granja C B, Aranguren L F, Vidal O M, Aragón L,Salazar M. Doeshyperthermia increase apoptosis in white spot syndrome virus (WSSV) infectedLitopenaeus vannamei?[J] Dis Aquat Org,2003,54:73—78
110、冯守明，杨先乐，李军等．凡纳滨对虾白斑综合征血液病理研究[J].水产学报2006，30(1):108-112
111、李继秋，谭北平，麦康森．白斑综合征病毒与凡纳滨对虾肠道菌群区系之间关系的初步研究[J].上海水产大学学报，2006，15(1):109-113
112、陈细法，吴定虎，黄槐等．对虾病毒病病毒与细菌合并感染的病理特点和诊断价值[J].病毒学报1997，13(2):146～150.
113、周勤，郭光远．弗劳地枸橼酸杆菌引起的猕猴腹泻[J].上海实验动物科学2004，24(1):39-40
114、陈翠珍，张晓君，房海等．中华绒螯蟹病原弗氏柠檬酸杆菌的鉴定[J].中国人兽共患病杂志2006，22(2):136-141
115、李华，邢殿楼，白国福等．弗氏柠檬酸杆菌对河蟹致病性的研究[J].水生生物学报2005，25（3）：217-223
116、冯迎春，颜其贵．从山羊鼻内腺瘤病例中分离出嗜麦芽窄食单胞菌的报告[J].畜牧与兽医2009，41（12）：81-83
117、耿毅，汪开毓，陈德芳等.斑点叉尾鮰一株致病菌的分离、鉴定与系统发育分析[J].微生物学报，2006，46(4):28-31
118、耿毅，汪开毓，黄小丽等．一种斑点叉尾鮰急性暴发性细菌性传染病初报[J].科学养鱼，2005，(3):51-52
119、周永灿，朱伟华．卵形鲳大规模个体病原及其防治[J].海洋科学，2001，(4):40-44.
120、黄斌，陈世峰．黄缘闭壳龟囊肿病的研究[J].淡水渔业，2002，(5):44-46
121、Harris N B, Rogers D G. Septicemia associated with Stenotrophomonasmaltophilia in a West African dwarf crocodile (Osteolaemus tetraspis subsp.tetraspis)[J]. Journal of Veterinary Diagnostic Investigation,2001,13(3):255-258
122、陈翠珍，房海，张晓君．石鲽病原琼氏不动杆菌形态型Ⅰ的鉴定[J].微生物学通报，2005，32(3)：34-39
123、陈翠珍，张晓君，房海等．锦鲤中琼氏不动杆菌的分离与鉴定[J].水生态学杂志，2009，2（1）：86-90
124、王祥红，李会荣，张晓华等.中国对虾成虾肠道微生物区系[J].青岛海洋大学学报，2000，30(3):493～498
125、Aoki T.Motile Aeromonads(AeromonashydroPhila).In CABIntemationa]，Fish Disease and disorders:Volume3;viral，baeterial and Fungalinfeetions (eds.Woo PTK and Bruno DW)，1999，427-453
126、Zhang X B, Huang C, Xu X, et al. Transcription and identification of anenvelope protein gene (p22)from shrimp white spot syndrome virus[J]. TheJournal of general virology,2002,83(2):471-477.
127、Huang C, Zhang X, Lin Q, et al. Proteomic analysis of shrimp white spotsyndrome viral proteins and characterization of a novel envelope proteinVP466[J].Mol Cell Proteomics,2002,1(3):223-231.
128、Faoud T Ishmael, Michael A Trakselis,Stephen J Benkovic.Protein-Protein Interactions in the Bacteriophage T4Replisome[J].The Journalof Biological Chemistry,2003,278(5):3145-3152.
129、Linda D Kosturko, Michael J Maggipinto, George Korza, et al.Heterogeneous Nuclear Ribonucleoprotein (hnRNP) E1Binds to hnRNP A2andInhibits Translation of A2Response Element mRNAs[J].Molecular Biology ofthe Cell,2006,17:3521-3533.
130、Yves Laumonnier, Tatiana Syrovets, Ladislva Burysek,et al. Identificationof the annexin A2heterotetramer as a receptor for the plasmin-induced signalingin human peripheral monocytes[J].Blood,2006,107:3342-3349.
131、Kristen E Neely, Ahmed H Hassan, Christine E Brown, et al. TranscriptionActivator Interactions with Multiple SWI/SNF Subunits[J].Molecular andCellular Biology,2002,22(6):1615-1625.
132、Pierre-Olivier Mari, Bogdan I Florea, Strphan P Persengiev,et al. Dynamicassembly of end-joining complexes requires interaction between Ku70/80andXRCC4[J].Proceedings of the National Academy of Sciences of the UnitedStates of America,2006,106:18597-18602.
133、梁艳，黄倢，易志刚等．对虾组织膜蛋白制备方法及其多肽组成[J].中国水产科学,2005,12(3):348-351.
134、Zhang X B, Huang C, Xu X, et al. Identification and localization of aprawn white spot syndrome virus gene that encodes an envelope protein[J].Journal of General Virology,2002,83:1069-1074.