早期静脉注射特布他林对大鼠急性肺损伤影响
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摘要
目的观察早期静脉注射特布他林注射液对大鼠急性肺损伤(ALI)的影响。
方法将40只SD大鼠随机分为4组：生理盐水对照组(A组)、ALI组(B组)、ALI+特布他林治疗组(C组)、ALI+特布他林+哇巴因干预组(D组),每组10只。每组大鼠分别以10%水合氯醛0.35 ml/100 g腹腔注射麻醉。麻醉成功后,A组大鼠采用尾静脉注射生理盐水1 ml/kg,1h内注完；B组大鼠采用尾静脉注射脂多糖(LPS)5 mg/kg(5 mg LPS融于1 ml生理盐水中),分4次给药,1 h内注完；C、D 2组大鼠采用尾静脉注射LPS 5 mg/kg(5 mg LPS融于1 ml生理盐水中),分4次给药,1 h内注射完后,再通过微量泵静脉注射浓度为10-4mol/L硫酸特布他林注射液0.5 ml/100 g体质量，1 h内注射完。然后将大鼠头部抬高45°,光源照射颈部直视下行气管插管,插管成功后,A、B、C 3组气管内滴注林格氏液1ml/kg,D组气管内滴注含哇巴因10-3mol/L的林格氏液1 ml/kg.造模后6 h,观察各组大鼠肺组织病理改变、并行肺组织病理学评分,测定各组大鼠的肺湿干重比(W/D),肺组织Na+-K+-ATP酶的活性,及大鼠肺泡灌洗液中的细胞数、中性粒细胞百分比及蛋白质含量。
结果A组肺组织病理学评分值、W/D值、肺泡灌洗液中细胞计数、中性粒细胞百分比、蛋白质含量(2.6±0.8、3.92±0.46、4.08±0.76×108、16.11±3.63%、0.17±0.07 g/L)均明显低于B、C、D 3组(分别为：12.2±3.2、5.12±0.48、10.67±1.84×108、47.2±6.03%、0.39±0.07 g/L；8.4±1.3、4.49±0.34、8.84±1.43×108、34.55±7.34%、0.28±0.06 g/L；11.3±1.7、4.98±0.31、10.67±1.61×108、44.93±7.51%、0.37±0.06 g/L；均P<0.05),B、D 2组上述指标均明显高于C组(均P<0.05),B组上述指标与D组比较,差异无统计学意义(P>0.05)。A组肺组织Na+-K+-ATP酶活性(18.188±2.081 U/mgprot)明显高于B、C、D 3组(分别为11.235±1.730 U/mgprot；14.211±1.658 U/mgprot；11.400±1.798 U/mgprot；均P<0.05),B、D 2组明显低于C组(均P<0.05),B组与D组比较,差异无统计学意义(P>0.05)。
结论早期静脉注射特布他林可能通过上调肺组织Na+-K+-ATP酶的活性来促进肺泡上皮细胞对液体重吸收,从而控制大鼠急性肺损伤的进展。
Objective To observe the effect of intravenous injection of terbutaline early on rats with acute lung injury.
Metheds 40 SD rats were randomly assigned into 4 groups:control group(group A), ALI group(group B), ALI+terbutaline treatment group(group C) and rats with acute lung injury, NS ALI+terbutaline+ouabain intervention group (group D),10 in every group. The rats of every group were respectively anesthetized by intraperitoneal injection of 10% Chloral Hydrate (0.35 ml/100 g). After being anaesthetized successfully, the rats in group A were injected 1 ml/kg NS by the tails vein within 1 hour; the rats in group B were injected 5 mg/kg LPS (which dissolved in 1 ml/5 mg of NS) by the tails vein in 4 times within 1 hour; the rats in group C and D were injected 5 mg/kg LPS (which dissolved in 1 ml/5 mg of NS) by the tails vein in 4 times within 1 hour, after then, the rats were injected 0.5 ml/100 g terbutaline sulfate injection (10-3 mol/L) by micro-pump within 1 hour. Then elevated the rats'head to 45 degrees, the tracheal intubation was performed under lights illuminating the rats'neck, after intubated successfully,1 ml/kg Ringer's solution was dripped into trachea in group A, B and C, and the group D with 1 ml/kg Ringer's solution which contain 10-3 mol/L ouabain. After 6 hours of establishing the model of acute lung injury in rats, pathomorphologic changes were observed, the pathomorphologic scores of lung injury were calculated, and the wet to dry weight ratio (W/D), and the activity of Na+-K+-ATPase, and bronchoalveolar lavage fluid (BALF) cells count, the percentage neutrophilicgranulocyte (PMN), BALF concentration of protein were determined.
Results The pathomorphologic scores of lung injury, the wet to dry weight ratio (W/D), bronchoalveolar lavage fluid (BALF) cells count, the percentage neutrophilicgranulocyte (PMN), BALF concentration of protein in group A (2.6±0.8、3.92±0.46、4.08±0.76×108、16.11±3.63%、0.17±0.07 g/L) were significantly lower than that in group B, C, D (12.2±3.2、5.12±0.48、10.67±1.84×108、47.2±6.03%、0.39±0.07 g/L; 8.4±1.3、4.49±0.34、8.84±1.43×108、 34.55±7.34%、0.28±0.06 g/L; 11.3±1.7、4.98±0.31、10.67±1.61×108、44.93±7.51%、0.37±0.06 g/L; all P<0.05), the above mentioned parameters in group B and D were higher than those in group C(all P<0.05), but there were no significant differences between group B and D(P>0.05), the activity of Na+-K+-ATPase in group A (18.188±2.081 U/mgprot)were significantly lower than that in group B, C, D (11.235±1.730 U/mgprot; 14.211±1.658 U/mgprot; 11.400±1.798 U/mgprot; all P<0.05), the activity of Na+-K+-ATPase in group B and D were higher than those in group C (all P<0.05), but there were no significant differences between group B and D (P>0.05).
Conclusion The intravenous injection of terbutaline early can promote alveolar epithelial fluid reabsorption in rats with ALI via the upregulation of the activity of Na+-K+-ATPase in lung,and the effect can control the further development of ALI.
方法将40只SD大鼠随机分为4组：生理盐水对照组(A组)、ALI组(B组)、ALI+特布他林治疗组(C组)、ALI+特布他林+哇巴因干预组(D组),每组10只。每组大鼠分别以10%水合氯醛0.35 ml/100 g腹腔注射麻醉。麻醉成功后,A组大鼠采用尾静脉注射生理盐水1 ml/kg,1h内注完；B组大鼠采用尾静脉注射脂多糖(LPS)5 mg/kg(5 mg LPS融于1 ml生理盐水中),分4次给药,1 h内注完；C、D 2组大鼠采用尾静脉注射LPS 5 mg/kg(5 mg LPS融于1 ml生理盐水中),分4次给药,1 h内注射完后,再通过微量泵静脉注射浓度为10-4mol/L硫酸特布他林注射液0.5 ml/100 g体质量，1 h内注射完。然后将大鼠头部抬高45°,光源照射颈部直视下行气管插管,插管成功后,A、B、C 3组气管内滴注林格氏液1ml/kg,D组气管内滴注含哇巴因10-3mol/L的林格氏液1 ml/kg.造模后6 h,观察各组大鼠肺组织病理改变、并行肺组织病理学评分,测定各组大鼠的肺湿干重比(W/D),肺组织Na+-K+-ATP酶的活性,及大鼠肺泡灌洗液中的细胞数、中性粒细胞百分比及蛋白质含量。
结果A组肺组织病理学评分值、W/D值、肺泡灌洗液中细胞计数、中性粒细胞百分比、蛋白质含量(2.6±0.8、3.92±0.46、4.08±0.76×108、16.11±3.63%、0.17±0.07 g/L)均明显低于B、C、D 3组(分别为：12.2±3.2、5.12±0.48、10.67±1.84×108、47.2±6.03%、0.39±0.07 g/L；8.4±1.3、4.49±0.34、8.84±1.43×108、34.55±7.34%、0.28±0.06 g/L；11.3±1.7、4.98±0.31、10.67±1.61×108、44.93±7.51%、0.37±0.06 g/L；均P<0.05),B、D 2组上述指标均明显高于C组(均P<0.05),B组上述指标与D组比较,差异无统计学意义(P>0.05)。A组肺组织Na+-K+-ATP酶活性(18.188±2.081 U/mgprot)明显高于B、C、D 3组(分别为11.235±1.730 U/mgprot；14.211±1.658 U/mgprot；11.400±1.798 U/mgprot；均P<0.05),B、D 2组明显低于C组(均P<0.05),B组与D组比较,差异无统计学意义(P>0.05)。
结论早期静脉注射特布他林可能通过上调肺组织Na+-K+-ATP酶的活性来促进肺泡上皮细胞对液体重吸收,从而控制大鼠急性肺损伤的进展。
Objective To observe the effect of intravenous injection of terbutaline early on rats with acute lung injury.
Metheds 40 SD rats were randomly assigned into 4 groups:control group(group A), ALI group(group B), ALI+terbutaline treatment group(group C) and rats with acute lung injury, NS ALI+terbutaline+ouabain intervention group (group D),10 in every group. The rats of every group were respectively anesthetized by intraperitoneal injection of 10% Chloral Hydrate (0.35 ml/100 g). After being anaesthetized successfully, the rats in group A were injected 1 ml/kg NS by the tails vein within 1 hour; the rats in group B were injected 5 mg/kg LPS (which dissolved in 1 ml/5 mg of NS) by the tails vein in 4 times within 1 hour; the rats in group C and D were injected 5 mg/kg LPS (which dissolved in 1 ml/5 mg of NS) by the tails vein in 4 times within 1 hour, after then, the rats were injected 0.5 ml/100 g terbutaline sulfate injection (10-3 mol/L) by micro-pump within 1 hour. Then elevated the rats'head to 45 degrees, the tracheal intubation was performed under lights illuminating the rats'neck, after intubated successfully,1 ml/kg Ringer's solution was dripped into trachea in group A, B and C, and the group D with 1 ml/kg Ringer's solution which contain 10-3 mol/L ouabain. After 6 hours of establishing the model of acute lung injury in rats, pathomorphologic changes were observed, the pathomorphologic scores of lung injury were calculated, and the wet to dry weight ratio (W/D), and the activity of Na+-K+-ATPase, and bronchoalveolar lavage fluid (BALF) cells count, the percentage neutrophilicgranulocyte (PMN), BALF concentration of protein were determined.
Results The pathomorphologic scores of lung injury, the wet to dry weight ratio (W/D), bronchoalveolar lavage fluid (BALF) cells count, the percentage neutrophilicgranulocyte (PMN), BALF concentration of protein in group A (2.6±0.8、3.92±0.46、4.08±0.76×108、16.11±3.63%、0.17±0.07 g/L) were significantly lower than that in group B, C, D (12.2±3.2、5.12±0.48、10.67±1.84×108、47.2±6.03%、0.39±0.07 g/L; 8.4±1.3、4.49±0.34、8.84±1.43×108、 34.55±7.34%、0.28±0.06 g/L; 11.3±1.7、4.98±0.31、10.67±1.61×108、44.93±7.51%、0.37±0.06 g/L; all P<0.05), the above mentioned parameters in group B and D were higher than those in group C(all P<0.05), but there were no significant differences between group B and D(P>0.05), the activity of Na+-K+-ATPase in group A (18.188±2.081 U/mgprot)were significantly lower than that in group B, C, D (11.235±1.730 U/mgprot; 14.211±1.658 U/mgprot; 11.400±1.798 U/mgprot; all P<0.05), the activity of Na+-K+-ATPase in group B and D were higher than those in group C (all P<0.05), but there were no significant differences between group B and D (P>0.05).
Conclusion The intravenous injection of terbutaline early can promote alveolar epithelial fluid reabsorption in rats with ALI via the upregulation of the activity of Na+-K+-ATPase in lung,and the effect can control the further development of ALI.
引文
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