中华哲水蚤种群遗传学研究
摘要
中华哲水蚤(Calanus sinicus)是西北太平洋陆架区的特征种,也是我国近海重要浮游桡足类之一。其在中国近海生态系统中的作用与地位与大西洋中的飞马哲水蚤(C. finmarchicus)相当。基于中华哲水蚤在海洋生态系统物流和能流中的重要地位,故被称为海洋浮游动物的“关键种”。半个世纪以来,在中华哲水蚤生物学、生态学等研究领域已积累了丰富的资料。但是,作为我国近海浮游动物的关键种,对中华哲水蚤的种群发生、增长及其补充过程和机制、生活史策略、生态对策等重大生物学和生态学问题我们仍知之甚少,许多问题诸如种群地理分化、渡夏机制等仍然悬而未决。研究中华哲水蚤的种群遗传学,是海洋生态系统动力学研究的一个重要组成部分,对于探索生态系统物质的输入和输出以及系统内部能量的运转机制具有重要意义,也是合理利用生物资源和资源保护的重要基础研究,也是研究全球环境变化对海洋生产力和产量影响的重要内容。
本文在中华哲水蚤线粒体COI基因片断序列的基础上,分析了中华哲水蚤的遗传多样性,遗传结构和特点,基因流模式;探讨不同地理种群(渤海,黄海,东海,台湾海峡和粤东沿岸)之间遗传差异以及在不同环境条件下种群分化的遗传学证据,探讨其生态策略等生态适应和生态进化等问题,为中华哲水蚤的种群补充、种群动力学研究以及地理分化提供科学依据。主要结果如下:
1. 2002年5月份在青岛胶州湾海域采获的中华哲水蚤的mtCOI基因片段长度为709 bp (国际基因库,索引号AY665663),其碱基组成A、C、G、T含量分别为24.26%、19.18%、22.43%和34.13%;C+G含量为41.61%,A+T为58.39%。与国际基因库登录(索引号AF332769)的中华哲水蚤(日本内海)mtCOI(633bp)比较,差异仅有3个位点,两者一致性(identity)达99.53%。
Calanus sinicus is a characteristic copepod widely distributed in Northwest Pacific continental shelf because it dominates the mesozooplankton in the shelf ecosystem, also components a major copepod in China seas for its eggs, larvae and adults provide a wide size spectrum of food to the commercial pelagic fish stocks. Owing to it plays a most important role in the material and energy flows in ecosystem of China seas, which corresponds to an other planktonic copepod Calanus finmarchicus inhabited in northern Atlantic, Calanus sinicus is named“key species”in China seas, especially in the Bohai Sea, the Yellow Sea and the East China Sea. Although Much biological and ecological research contribution (e.g. Feeding, fecundity, development, growth, diel vertical migration, spatio-temporal distribution, seasonal life cycle, etc.) of Calanus sinicus has been well documented in previous field and laboratory investigations in the past about fifty years, little research field has been dealed with the maintenance mechanism of Calanus sinicus population dynamics, especial in population genetic diversity in China seas. We have little knowledge on the significant problems as follow as life strategy, ecological adaptation and ecological evolution, population geodiversity and differentiation, as well as oversummering of Calanus sinicus. Studies on the population genetics of Calanus sinicus is an important research field in marine ecosystem dynamic project. Population genetic analysis may yield statistical rather than deterministic conclusions about population dynamic phenomena. Thus, the destination of a particular plankton may not be predictable, but how frequently a dispersal pathway is used (i.e. estimation of the number of migrating individuals) may be established using population genetic statistics
本文在中华哲水蚤线粒体COI基因片断序列的基础上,分析了中华哲水蚤的遗传多样性,遗传结构和特点,基因流模式;探讨不同地理种群(渤海,黄海,东海,台湾海峡和粤东沿岸)之间遗传差异以及在不同环境条件下种群分化的遗传学证据,探讨其生态策略等生态适应和生态进化等问题,为中华哲水蚤的种群补充、种群动力学研究以及地理分化提供科学依据。主要结果如下:
1. 2002年5月份在青岛胶州湾海域采获的中华哲水蚤的mtCOI基因片段长度为709 bp (国际基因库,索引号AY665663),其碱基组成A、C、G、T含量分别为24.26%、19.18%、22.43%和34.13%;C+G含量为41.61%,A+T为58.39%。与国际基因库登录(索引号AF332769)的中华哲水蚤(日本内海)mtCOI(633bp)比较,差异仅有3个位点,两者一致性(identity)达99.53%。
Calanus sinicus is a characteristic copepod widely distributed in Northwest Pacific continental shelf because it dominates the mesozooplankton in the shelf ecosystem, also components a major copepod in China seas for its eggs, larvae and adults provide a wide size spectrum of food to the commercial pelagic fish stocks. Owing to it plays a most important role in the material and energy flows in ecosystem of China seas, which corresponds to an other planktonic copepod Calanus finmarchicus inhabited in northern Atlantic, Calanus sinicus is named“key species”in China seas, especially in the Bohai Sea, the Yellow Sea and the East China Sea. Although Much biological and ecological research contribution (e.g. Feeding, fecundity, development, growth, diel vertical migration, spatio-temporal distribution, seasonal life cycle, etc.) of Calanus sinicus has been well documented in previous field and laboratory investigations in the past about fifty years, little research field has been dealed with the maintenance mechanism of Calanus sinicus population dynamics, especial in population genetic diversity in China seas. We have little knowledge on the significant problems as follow as life strategy, ecological adaptation and ecological evolution, population geodiversity and differentiation, as well as oversummering of Calanus sinicus. Studies on the population genetics of Calanus sinicus is an important research field in marine ecosystem dynamic project. Population genetic analysis may yield statistical rather than deterministic conclusions about population dynamic phenomena. Thus, the destination of a particular plankton may not be predictable, but how frequently a dispersal pathway is used (i.e. estimation of the number of migrating individuals) may be established using population genetic statistics
引文
1. 毕洪生,孙 松,高尚武,张光涛. 渤海浮游动物群落生态特点 Ⅱ: 桡足类数量分布及变动 [J]. 生态学报, 2001,21(2): 177～185
2. 曹文清. 厦门港海区两种常见纺锤水蚤染色体研究 [J].厦门大学学报(自然科学版), 1994a,33(增刊): 125～128
3. 曹文清,张跃军. 火腿许水蚤染色体组型的初步研究 [J]. 厦门大学学报(自然科学版), 1994b,33(6): 853～856
4. 曹文清,杨 明,谭树华,林元烧,郭东晖. 中华哲水蚤不同地理种群苹果酸脱氢酶(MDH)的比较 [J]. 海洋科学, 2002,26(8): 18～19
5. 陈清潮. 中华哲水蚤的繁殖、性比率和个体大小的研究 [J]. 海洋与湖沼, 1964, 6(3): 272～288
6. 陈世杰. 桡足类的功能适应与生态对策 [J]. 福建水产, 1994, 4: 30～35
7. 柴心玉,钱树本,张 庆. 长江口及济州岛邻近海域叶绿素-a分布及其与水团、跃层的相关性 [J]. 青岛海洋大学学报,1991,21(2): 69～81
8. 陈晓丹,王真良. 北黄海中华哲水蚤各期幼体昼夜垂直移动的初步研究 [J]. 海洋学报, 1991, 13(2): 247～253
9. 赫崇本,汪园祥,雷宗友,徐 斯. 黄海冷水团的形成及其性质的初步探讨 [J]. 海洋与湖沼,1959, 2(1): 11～15
10. 黄加祺,郑重. 温度和盐度对厦门港几种桡足类存活率的影响 [J]. 海洋与湖沼,1986,17(2):161～167
11. 黄加祺,李少菁,陈亚萍. 福建罗源湾浮游动物的种类组成和数量分布 [J]. 厦门大学学报(自然科学版),1989,28(福建省罗源湾海域综合调查研究专辑):85～95
12. 黄加祺,李少菁,陈 钢. 台湾海峡及其邻近海域中华哲水蚤的分布和繁殖 [J]. 海洋科学集刊,2002,44:95～100
13. 管秉贤. 黄海冷水团的水温变化及环流特征的初步分析 [J]. 海洋与湖沼,1963,5(4):225～281
14. 管秉贤. 东海海流结构及涡旋特征概述 [J]. 海洋科学集刊,1986,27: 1～21
15. 高天翔,任一平,张秀梅,渡边精一. 日本绒螯蟹线粒体DNA序列研究 II. 16S rRNA [J]. 青岛海洋大学学报. 2000, 30(3): 482～486
16. 高天翔,张秀梅,张军生. 齿突斜纹蟹线粒体DNA中12S rRNA基因序列的研究 [J]. 大连水产学院学报,2000,15(2): 98～102
17. 高天翔,吉崎悟朗,渡边精一. 中华绒螯蟹线粒体DNA 12S rRNA基因片段的序列研究 [J]. 中国水产科学,2000,7(3): 1～4
18. 福建海洋研究所. 1988. 台湾海峡中、北部海洋综合调查研究报告 [M]. 科学出版社
19. 李少菁. 福建沿海太平洋哲镖蚤 (Calanus pacificus Brodsky) 的比较形态研究 [J]. 厦门大学学报 (自然科学版),1963,10(1): 57～81
20. 李少菁. 厦门几种海洋浮游桡足类的食性与饵料成分的初步研究 [J]. 厦门大学学报 (自然科学版),1964,11(3): 93～109
21. 李少菁,陈 峰,王桂忠. 厦门海区浮游桡足类卵形态与孵化率的研究 [J]. 厦门大学学报(自然科学版),1989,28(4): 538～543
22. 李永明,赵玉琪等著. 2000. 实用分子生物学方法手册 [M]. 科学出版社
23. 林景宏,陈瑞祥. 东山湾浮游桡足类的分布 [J]. 海洋通报,1992,11(4):41～46
24. 林元烧,李 松. 厦门港中华哲水蚤 (Calanus sinicus Brodsky) 生活周期的初步研究 [J]. 厦门大学学报 (自然科学版), 1984, 23: 111～117
25. 林元烧,李 松. 厦门港中华哲水蚤产卵量的初步研究 [J]. 厦门大学学报 (自然科学版), 1986,25(1): 108～112
26. 林元烧,曹文清,姚津津. 厦门港中华哲水蚤染色体组型[J]. 厦门大学学报(自然科学版),2000,39(6):826～830
27. 林元烧,曹文清,方旅平,刘茜茜,李少菁. 中华假磷虾线粒体DNA COI基因片段序列分析 [J]. 厦门大学学报(自然科学版),2004,43(6): 842～846
28. 林元烧,方旅平,曹文清,李少菁. 中华哲水蚤线粒体 COI 序列分析 [J]. 厦门大学学报(自然科学版),2005,44(1): 90～93
29. 刘文臣,王 荣,吉 鹏. 东海颗粒有机碳的研究 [J]. 海洋与湖沼,1997,28:39～43
30. 毛汉礼,甘子钧,蓝淑芳. 长江冲淡水及其混合问题的初步研究 [J]. 海洋与湖沼, 1963, 5(3): 183～205
31. 毛 连 菊 , 张 从 尧 , 赵 文 . 二 种 桡 足 类 的 染 色 体 组 型 分 析 [J]. 中 国 水 产 科学,2002,9(2):129～132
32. 门荣新,杨官品,沈锡权,廖梅杰,管晓菁. 胶州湾浮游桡足类18S 核糖体RNA基因序列变异分析 [J]. 中国海洋大学学报(自然科学版),2005,35(3):392～396
33. 沈嘉瑞,白雪娥. 烟台鲐鱼产卵场桡足类的研究 [J]. 动物学报, 1956, 8(2): 177～182
34. 沈国英,施并章. 海洋生态学(修订版)[M]. 厦门:厦门大学出版社,1990
35. 苏纪兰,黄大吉. 黄海冷水团的环流结构 [J]. 海洋与湖沼,1995,26(5): 1～7
36. 苏纪兰,袁业立. 2005. 中国近海水文 [M]. 北京: 海洋出版社
37. 苏育嵩. 黄东海地理环境概括、环流系统与中心渔船 [M]. 山东海洋学院学报,1986,16(1):12～27
38. 孙 松, 王 荣, 张光涛,杨 波,吉 鹏,张 芳. 黄海中华哲水蚤度夏机制初探 [J]. 海洋与湖沼, 2002 (浮游动物研究专辑): 92～99
39. 孙乃恩,孙东旭,朱德煦. 分子遗传学 [M]. 南京大学出版社,1990.pp.7～9
40. 唐启升,苏纪兰等. 2000. 中国海洋生态系统动力学研究 I:关键科学问题于研究发展战略 [M]. 科学出版社
41. 谭树华,林元烧,曹文清,陈钢,杨明. 黄、东海中华哲水蚤种群遗传的初步研究 I: 等位酶分析 [J]. 厦门大学学报(自然科学版),2003, 42(1): 87～91
42. 谭树华,曹文清,林元烧,李少菁,郭东晖. 黄、东海精致真刺水蚤种群遗传结构研究 [J]. 海洋科学,2004,28(4): 29～33
43. 徐兆礼,崔雪森,陈卫忠. 东海浮游桡足类的种类组成及优势种 [J]. 水产学报,2004,28(1): 35～40
44. 王桂忠,李少菁. 厦门港海区真刺唇角水蚤不同季节种群的同工酶分析 [J]. 海洋与湖沼. 1992, 23(4):657～662
45. 王 荣,张 经,黄大吉,孟田湘,金显仕. 2000. 东、黄海生态系统的基本特征. 引自:唐启升,苏纪兰等著. 中国海洋生态系统动力学研究 I: 关键科学问题与研究发展战略 [M]. 科学出版社,2000. pp. 34～42
46. 王 荣,陈亚瞿. 浮游动物种群的补充. 引自:唐启升,苏纪兰等著. 中国海洋生态系统动力学研究 I: 关键科学问题与研究发展战略 [M]. 科学出版社,2000. pp. 107～109
47. 王 荣,陈亚瞿,王 克,左 涛. 东海中华哲水蚤的年产量估算 [J]. 生态学报,2003, 23(6):1213～1215
48. 王新刚, 孙 松, 蒲新明, 张芳. 黄海冷水团区域中华哲水蚤的体长、体重、元素组成和代谢特征 [J]. 海洋与湖沼, 2002, (浮游动物研究专辑), pp. 45～50
49. 王雄进,康洁生,李 松. 厦门港中华哲水蚤体长、体重和碳、氮、氢含量的季节变化 [J]. 台湾海峡,1988,7(2): 173～179
50. 王雄进,康洁生,李 松. 厦门港中华哲水蚤的体长、体重、化学组成和呼吸率的研究 [J]. 甲壳动物学论文集,1990,2: 53～64
51. 王中仁. 1996. 植物等位酶分析 [M]. 科学出版社
52. 杨纪明. 渤海中华哲水蚤摄食的初步研究 [J]. 海洋与湖沼. 1997, 28(4): 376～382
53. ------. 渤海中华哲水蚤桡足幼体摄食的初步研究 [J]. 海洋水产研究, 1998,19(2): 1～4
54. 张 芳,孙 松. 中华哲水蚤生态学研究进展 [J]. 海洋科学, 2001, 25(11): 16～19
55. 张光涛,孙 松,张 芬,刘桂梅. 度夏过程中温度对中华哲水蚤生殖的伤害作用 [J]. 海洋与湖沼,2002,(浮游动物研究专辑): 85～90
56. 张秀梅,高天翔,王溪宏. 冲绳日本绒螯蟹线粒体 DNA 12S rRNA 序列的研究 [J]. 湛江海洋大学学报, 2000, 20(2): 11～15
57. 郑元甲,陈雪忠,程家骅,王云龙,沈新强,陈卫忠,李长松等. 东海大陆架生物资源与环境 [M]. 上海科学技术出版社,2003. pp.169～205
58. 郑重,郑执中. 十年来我国浮游动物的研究 [J]. 海洋与湖沼,1959,2(1): 214～222
59. 郑重. 厦门海洋浮游动物的初步研究 [J]. 厦门大学学报(自然科学版),1955,5: 1～16
60. 郑重,李少菁,连光山. 1992. 海洋桡足类生物学 [M]. 厦门:厦门大学出版社
61. 周美玉,林元烧,方旅平,郑连明,刘迟迟,曹文清. 甲醛溶液保存的中华哲水蚤基因组 DNA 提取和 PCR 扩增 [J]. 厦门大学学报(自然科学版),2005,44(3): 403～406
62. 祖元刚,孙 梅,康 乐. 生态适应与生态进化 [M]. 北京:高等教育出版社,施普林格出版社,2000
63. Battaglia B, Bisol PM and V Varotto. Genetic variability in marine and brackish populations of Tisbe holothuriae (Copepoda; Harpactacoida) [J]. Archaeological Zoology and Experimental Genetics. 1978.119:251-264
64. Bucklin A, Caudill CC and M Guarnieri. 1998. Population genetics and phylogeny of planktonic copepods. In: Molecular Approaches to the Study of the Ocean [M]. Edited by Keith E. Cooksey. Chapman and Hall (London). pp. 303-317
65. Bucklin A and TC Lajeunesse. Molecular genetic variation of Calanus pacificus (Copepod: Calanoida): preliminary evaluation of genetic structure and subspecific differentiation based on mtDNA sequence [C]. California Cooperative Oceanic Fisheries Investigation Reports, 1994, 25:45-51
66. Bucklin A and PH Wiebe. Low mitochondrial diversity and small effective population sizes of the copepods, Calanus finmarchicus and Nannocalanus minor: possible impact of climatic variation during recent glaciation [J]. J. Heredity, 1998, 89:383: 392
67. Bucklin A, Smolenack SB, Bentley AM and PH Wiebe. Gene flow patterns of the euphausiid, Meganyctiphanes norvegica, in the N Atlantic based on DNA sequences for mitochondrial cytochondrial cytochrome oxidase I and bytochrome b [J]. J. Plankton Res., 1997, 19: 1763-1791
68. Bucklin A. Methods for population genetic analysis of zooplankton [M]. ICES Zooplankton Methodology Manual. Acadimic Press. 2000
69. Bucklin A and Marcus NH. Genetic differentiation of populations of the planktonic copepod Labidocera aestiva [J]. Mar. Biol., 1985, 84: 219-224
70. Bucklin A, Reinecker MM and CNK Mooers. Genetic tracers of zooplankton transport in coastal filaments of the US west coast [J]. J. Geophys. Res., 1989, 94: 8277-8288
71. Bucklin A. Population genetic responses of the planktonic copepod Metredia pacifica to acoastal eddy in the California Current [J]. J. Geophys. Res., 1991, 96: 14799-14808
72. Bucklin A, Frost BW and TD Kocher. DNA sequence variation of the mitochondrial 16S rRNA in Calanus (Copepoda; Calanoida): intraspecific and interspecific patterns [J]. Molec. Mar. Biol. Biotech., 1992. 1: 397-407
73. Bucklin A, Frost BW and TD Kocher. Molecular systematics of seven species of Calanus and three species of Metridia species (Calanodia:Copepoda) [J]. Mar. Biol., 1995, 121:655-664
74. Bucklin A, Sundt R and G Dahle. The population genetics of Calanus finmarchicus in the North Atlantic. Proceedings of an ICES planning workshop for a TransAtlantic Study of Calanus finmarchicus [J]. Ophelia,1996, 44: 29-45.
75. Bucklin A and TD Kocher. Source regions for recruitment of Calanus finmarchicus to Georges Bank: evidence from molecular population genetic analysis of mtDNA [J]. Deep-Sea Research II. 1996, 43(7-8):1665-1681
76. Bucklin A, LaJeunesse TC, Curry E, Wallinga J and K Garrison. Molecular genetic diversity of the copepod, Nannocalanus minor: genetic evidence of species and population structure in the N. Atlantic Ocean [J]. J. Marine Research, 1996, 54: 285-310
77. Bucklin A, Bentley AM and SP Franzen. Distribution and relative abundance of the copepods, Pseudocalanus moultoni and P. newmani, on Georges Bank based on molecular identification of sibling species [J]. Mar. Biol, 1998, 132: 97-106
78. Bucklin A, Wiebe PH, Smolenack SB, Copley NJ and ME Clarke. Integrated biochemical, molecular genetic, and bioacoustical analysis of mesoscale variability of the euphausiid Nematoscelis difficilis in the California Current [J]. Deep-Sea Res., 2002, 49: 437-462
79. Bucklin A, Frost BW, Bradford-Grieve J, Allen LD and NJ Copley. Molecular systematic and phylogenetic assessment of 34 Calanoid copepod species of the Calanidae and Clausocalanidae [J]. Marine Biology, 2003, 142: 333-343.
80. Burton RS and SG Swisher. Population structure of the intertidal copepod Tigriopus californicus as revealed by field manipulation of allele frequencies [J]. Ocealogia., 1984. 65: 108-111
81. Burton RS. Evolutionary consequences of restricted gene flow among natural populations of the copepod, Tigriopus californicus [J]. Bull. Mar. Sci., 1986, 39: 526-535
82. Burton RS and BN Lee. Nuclear and mitochondrial gene genealogies and allozyme polymorphism across a major phylogeographic break in the copepod Tigriopus californicus [C]. Proc. Natl. Acad. Sci. USA, 1994, 91: 5197-5201
83. Caudill CC. 1995. Molecular evidence of population genetic differentiation and sibling species in Acartia tonsa (Copepoda: Calanoida) [T]. MA Thesis, Department of Zoology, University of New Hampshire, Durham, NH.
84. Clary DO and DR Wolstenholme. The mitochondrial DNA molecule of Drosophila yakuba: nucleotide sequence, gene organization and genetic code [J]. J. Mol. Evol., 1985, 22:252-271.
85. Chen QC. The marine zooplankton of Hong Kong [C]. In: Morton, BS and Tseng CK (eds), Proceedings of the First International Marine biological Workshop: The Marine Flora and Fauna of Hong Kong and Southern China. Hong Kong University Press, Hong Kong, pp.789-799
86. Chiba T. On the distribution of the plankton in the Eastern China Sea and Yellow Sea [J]. Bull. Japan Soc. Sci. Fisher., 1952, 17(7): 183-190
87. Corkett CJ and IA McLaren. 1978. The biology of Pseudocalanus [J]. Advances in Marine Biology, vol. 15. pp.1-233
88. Edmands S. Phylogeography of the intertidal copepod Tigriopus californicus reveals substantially reduced population differentiation at northern latitudes [J]. Molecular Ecol. 2001, 10: 1743-1750
89. Ekman S. 1953. Zoogeography of the sea [M]. London. pp. 321-324.
90. Enomoto Y. Studies on the food base in the Yellow and the East China Sea III. Notes on some dominant zooplankton [J]. Bull. Japan Soc. Sci. Fisher., 1963, 29(1): 7-13.
91. Folmer O, Black M, Hoen W, Lutz R and R Vrijenhoek. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates [J]. Molec. Mar. Biol. Biotech.,1994,3:294～299.
92. Humes AG. How many copepods? [J]. Hydrobiologia, 1994, 292/293: 1-7
93. Goswami U and Goswami SC. Karyological studies in the genus Acartia (copopoda) [J]. Current Science, 1973, 42(7): 242-243
94. ---------------------------------------. Karyology of the genus Labidocera Lubbock from the Laccadive Sea (Lakshadweep) [J]. Indian Journal of Marine Sciences, 1979, 8:259-261
95. ---------------------------------------. Chromosomal aberrations found in Paracalanus aculeatus (Gesbrecht) at the time of solar eclipse [J]. Mahasagar Bulletin of the National Institute of Oceanography, 1982, 15(1):59-62
96. --------------------------------------. A note on chromosomes of Pontellopsis herdmani and Pontella princes (Copepoda) from the Laccadive Sea [J]. Mahasagar Bulletin of the National Institute of Oceanography, 1984, 17(2):129-132
97. Guarnieri M. 1996. A preliminary study of genetic variation within and among populations of Diaptomus leptopus (Copepoda: Calanoida): a DNA sequence analysis of the mitochondrial cytochrome oxidase I gene [T]. MA Thesis, Department of Biology, Concordia University, Montreal, Quebec.
98. Han XF, Wang R and JP Wang. Digestive gut structure and activity of protease, amylase, and alkaline phosphatase in Calanus sinicus during summer in the Yellow Sea and the East China Sea [J]. J. Exp. Mar. Biol. Ecol., 2002, 270: 131-146
99. Hill SR, Allen LD and A Bucklin. Multiplexed species-specific PCR protocol to discriminate four N. Atlantic Calanus species, with an mtCOI gene tree for ten Calanus species [J]. Mar. Biol., 2001, 139: 279-287
100. Hirose E, Toda H, Saito Y and H Watanabe. Formation of the multiple-layered fertilization envelope ini the embryo of Calanus sinicus Brodsky (Copepoda: Calanoida) [J]. J. Crust. Biol. 1992, 12(2): 186-192
101. Hudson RR, Slatkin, M and WP Maddison. Estimation of levels of gene flow from DNA sequence data [J]. Genetics, 1992, 132: 583-589
102. Huang C, Uye S and T Onbe. Geographic distribution, seasonal life cycle, biomass and production of a planktonic copepod Calanus sinicus in the Inland Sea of Japan and its neighboring Pacific Ocean [J]. J. Plankton Res., 1993, 15: 1229-1246
103. Hulsemann K. Calanus sinicus Brodsky and C. jashnovi, nom. Nov (Copepoda: Calanoida) of the North-west Pacific Ocean: a comparison, with notes on the integumental pore pattern in Calanus s. str. [J]. Invertevr. Taxon, 1994, 8:1461-1482
104. Hwang JS, Chen QC and CK Wong. Taxonomic composition and grazing rate of calanoid copepods in coastal waters of northern Taiwan [J]. Crustaceana, 1998, 71: 378-389
105. Hwang JS, Chen QC and CK Wong. Taxonomic composition, density and biomass of free-living copepods in the coastal waters of southwestern Taiwan [J]. Crustaceana, 2003, 76: 193-206
106. Hwang JS and CK Wong. The China coastal current as a driving force for transporting Calanus sinicus (Copepoda: Calanoida) from its population centers to waters off Taiwan and Hong Kong during the winter northeast monsoom period [J]. J Plankton Res., 2005, 27(2): 205-210
107. Imabayashi H and T Endo. Distribution o near-bottom plankton in the Ohta River estuary, Hiroshima Bay, in relation to salinity [J]. Bulletin of the Plankton Society of Japan, 1986,33: 113-123
108. Jarman SN, Elliott NG, Nicol S and A McMinn. Molecular phylogenetics of circumglobal Euphausia species (Euphausiacea: Crustacea) [J]. Can. J. Fish. Aquat. Sci., 2000, 57 (Suppl. 3): 51-58
109. Kann LM and Wishner K. Genetic population structure of the copepod Calanus finmarchicus in the gulf of Maine: allozyme and amplified mitochondrial DNA variation [J]. Mar. Biol. 1996. 125: 65-75
110. Lan YC, Shih CT, Lee MA and HZ Shieh. Spring distribution of copepods in relation to water masses in the Northern Taiwan Strait [J]. Zoological Studies, 2004, 43(2): 332-343
111. Lazzaretto I and A Libertini. Karyological investigations of two populations of Tigriopus (Copepoda, Harpacticoida) from the Kerguelen region [J]. Curstacean Biology, 1985, 5(2):330-333
112. Lee CE. Global phylogeography of a cryptic copepod species complex and reproductive isolation between genetically proximate “populations” [J]. Evolution, 2000, 54(6): 2014-2027
113. Li C, Sun S, Wang R and X wang. Feeding and respiration rates of a planktonic copepod (Calanus sinicus) oversummering in Yellow Sea Cold Bottom Waters [J]. Mar. Biol., 2004, 145: 149-157
114. Lindeque PK, Harris RP, Jones MB and GR Smerdon. Simple molecular method to distinguish the identity of Calanus pecies (Copepoda:Calanoida) at any development stage[J]. Mar. Biol., 1999, 133:91-96.
115. Manning CA & A Bucklin. Multivariate ananysis of the copepod community of near-shore waters in the western Gulf of Maine [J]. Mar. Ecol. Progr. Ser., 2005, 292: 233-249
116. Marshall SM and Orr AP. 1955. The biology of a marine copepod, Calanus finmarchicus (Gunnerus) [M]. London: Oliver & Boyd.
117. Mauchline J, Blaxter JHS, Southward AJ and PA Tyler. 1998. The biology of Calanoid Copepods [M]. Advances in Marine Biology, vol. 33. pp.1-701.
118. Machida RJ, Miya MU, Nishida M and S Nishida. Complete mitochondrial DNA sequence of Tigriopus japonicus (Crustacea:Copepoda) [J]. Marine Biotechnolology, 2002, 4: 406-417.
119. Mori T. 1937. The pelagic copepods from the neighbouring waters of Japan [M]. Tokyo. pp. 150.
120. Nei M and S Kumar. 2000. Molecular evolution and phylogenetics [M]. Oxford University Press, Inc. [吕宝忠,钟 杨,高莉萍等译. 2002. 分子进化与系统发育 [M].高等教育出版社]
121. Roff DA and P Bentzen. The statistical analysis of mitochondrial DNA polymorphisms: X2 and the problem of small samples [J]. Mol. Biol. Evol., 1989, 6: 539-545
122. Page RDM and EC Holmes. 2000. Molecular evolution, a phylogenetic approach [M]. Blackwell Sc. Press, Inc.
123. Pu XM, Sun S, Yang B, Ji P, Zhang YS and F Zhang. The combined effects of temperature and food supply on Calanus sinicus in the southern Yellow Sea in summer [J]. J Plankton Res., 2004, 26(9): 1049-1057
124. Pu XM, Sun S, Yang B, Zhang GT and F Zhang. Life history strategies of Calanus sinicus in the southern Yellow Sea in summer [J]. J. Plankton Res., 2004, 26(9): 1059-1068
125. Schizas NV, Chandler GT and BC Coull. Differential survival of three mitochondrial lineages of a marine benthic copepod exposed to a pesticide mixture [J]. Environ. Sci. Tech.,2001, 35(3): 535-538
126. Sevigny JM and Odense P. Comparison of isoenzyme systems of Calanoid copepods by use of ultrathin agarose gel isoelectric focusing techniques [J]. Comp. Biochem. Physiol., 1985,80B: 455-461
127. Sevigny JM and IA McLaren. Protein polymorphism in six species of the genus Calanus [J]. Hydrobiologia, 1988, 167/168: 275-284
128. Sevigny JM, McLaren IA and BW Frost. Discrimination among and variation within species of Pseudocalanus Based on the GPI locus [J]. Mar. Biol., 1989, 102: 321-327
129. Sproston NG. Preliminary survey of the plankton of the Chu-San region, with a review of the relevant literature [J]. Sinensia, 1949, 20: 151-154.
130. Toda H and E Hirose. SEM and TEM observations on egg membranes of the two types of Calanus sinicus Eggs [J]. Proceedings of the fourth Intl. Conference on Copepoda; Bull. Plankton Soc. Japan, Spec.Vol. (1991): 613-617
131. Uye S. Temperature-dependent development and growth of Calanus sinicus (Copepoda: Calanoida) in the laboratory [J]. Hydrobiologia, 1988,167/168: 285-293
132. -------. Why does Calanus sinicus prosper in the shelf ecosystem of the Northwest Pacific Ocean? [J]. ICES Journal Marine Science, 2000, 57 (6): 1850-1855
133. Wang R, Zou T and K Wang. The Yellow Sea cold bottom water-an oversummering site for Calanus sinicus (Copepoda, Crustacea) [J]. J. Plankton res., 2003, 25, 169-183
134. Wong CK, Hwang JS and QC Chen. Taxonomic composition and grazing impact of calanoid copepods in coastal waters near nuclear power plants in northern Taiwan [J]. Zool. Stud., 1998, 37:330-339
2. 曹文清. 厦门港海区两种常见纺锤水蚤染色体研究 [J].厦门大学学报(自然科学版), 1994a,33(增刊): 125～128
3. 曹文清,张跃军. 火腿许水蚤染色体组型的初步研究 [J]. 厦门大学学报(自然科学版), 1994b,33(6): 853～856
4. 曹文清,杨 明,谭树华,林元烧,郭东晖. 中华哲水蚤不同地理种群苹果酸脱氢酶(MDH)的比较 [J]. 海洋科学, 2002,26(8): 18～19
5. 陈清潮. 中华哲水蚤的繁殖、性比率和个体大小的研究 [J]. 海洋与湖沼, 1964, 6(3): 272～288
6. 陈世杰. 桡足类的功能适应与生态对策 [J]. 福建水产, 1994, 4: 30～35
7. 柴心玉,钱树本,张 庆. 长江口及济州岛邻近海域叶绿素-a分布及其与水团、跃层的相关性 [J]. 青岛海洋大学学报,1991,21(2): 69～81
8. 陈晓丹,王真良. 北黄海中华哲水蚤各期幼体昼夜垂直移动的初步研究 [J]. 海洋学报, 1991, 13(2): 247～253
9. 赫崇本,汪园祥,雷宗友,徐 斯. 黄海冷水团的形成及其性质的初步探讨 [J]. 海洋与湖沼,1959, 2(1): 11～15
10. 黄加祺,郑重. 温度和盐度对厦门港几种桡足类存活率的影响 [J]. 海洋与湖沼,1986,17(2):161～167
11. 黄加祺,李少菁,陈亚萍. 福建罗源湾浮游动物的种类组成和数量分布 [J]. 厦门大学学报(自然科学版),1989,28(福建省罗源湾海域综合调查研究专辑):85～95
12. 黄加祺,李少菁,陈 钢. 台湾海峡及其邻近海域中华哲水蚤的分布和繁殖 [J]. 海洋科学集刊,2002,44:95～100
13. 管秉贤. 黄海冷水团的水温变化及环流特征的初步分析 [J]. 海洋与湖沼,1963,5(4):225～281
14. 管秉贤. 东海海流结构及涡旋特征概述 [J]. 海洋科学集刊,1986,27: 1～21
15. 高天翔,任一平,张秀梅,渡边精一. 日本绒螯蟹线粒体DNA序列研究 II. 16S rRNA [J]. 青岛海洋大学学报. 2000, 30(3): 482～486
16. 高天翔,张秀梅,张军生. 齿突斜纹蟹线粒体DNA中12S rRNA基因序列的研究 [J]. 大连水产学院学报,2000,15(2): 98～102
17. 高天翔,吉崎悟朗,渡边精一. 中华绒螯蟹线粒体DNA 12S rRNA基因片段的序列研究 [J]. 中国水产科学,2000,7(3): 1～4
18. 福建海洋研究所. 1988. 台湾海峡中、北部海洋综合调查研究报告 [M]. 科学出版社
19. 李少菁. 福建沿海太平洋哲镖蚤 (Calanus pacificus Brodsky) 的比较形态研究 [J]. 厦门大学学报 (自然科学版),1963,10(1): 57～81
20. 李少菁. 厦门几种海洋浮游桡足类的食性与饵料成分的初步研究 [J]. 厦门大学学报 (自然科学版),1964,11(3): 93～109
21. 李少菁,陈 峰,王桂忠. 厦门海区浮游桡足类卵形态与孵化率的研究 [J]. 厦门大学学报(自然科学版),1989,28(4): 538～543
22. 李永明,赵玉琪等著. 2000. 实用分子生物学方法手册 [M]. 科学出版社
23. 林景宏,陈瑞祥. 东山湾浮游桡足类的分布 [J]. 海洋通报,1992,11(4):41～46
24. 林元烧,李 松. 厦门港中华哲水蚤 (Calanus sinicus Brodsky) 生活周期的初步研究 [J]. 厦门大学学报 (自然科学版), 1984, 23: 111～117
25. 林元烧,李 松. 厦门港中华哲水蚤产卵量的初步研究 [J]. 厦门大学学报 (自然科学版), 1986,25(1): 108～112
26. 林元烧,曹文清,姚津津. 厦门港中华哲水蚤染色体组型[J]. 厦门大学学报(自然科学版),2000,39(6):826～830
27. 林元烧,曹文清,方旅平,刘茜茜,李少菁. 中华假磷虾线粒体DNA COI基因片段序列分析 [J]. 厦门大学学报(自然科学版),2004,43(6): 842～846
28. 林元烧,方旅平,曹文清,李少菁. 中华哲水蚤线粒体 COI 序列分析 [J]. 厦门大学学报(自然科学版),2005,44(1): 90～93
29. 刘文臣,王 荣,吉 鹏. 东海颗粒有机碳的研究 [J]. 海洋与湖沼,1997,28:39～43
30. 毛汉礼,甘子钧,蓝淑芳. 长江冲淡水及其混合问题的初步研究 [J]. 海洋与湖沼, 1963, 5(3): 183～205
31. 毛 连 菊 , 张 从 尧 , 赵 文 . 二 种 桡 足 类 的 染 色 体 组 型 分 析 [J]. 中 国 水 产 科学,2002,9(2):129～132
32. 门荣新,杨官品,沈锡权,廖梅杰,管晓菁. 胶州湾浮游桡足类18S 核糖体RNA基因序列变异分析 [J]. 中国海洋大学学报(自然科学版),2005,35(3):392～396
33. 沈嘉瑞,白雪娥. 烟台鲐鱼产卵场桡足类的研究 [J]. 动物学报, 1956, 8(2): 177～182
34. 沈国英,施并章. 海洋生态学(修订版)[M]. 厦门:厦门大学出版社,1990
35. 苏纪兰,黄大吉. 黄海冷水团的环流结构 [J]. 海洋与湖沼,1995,26(5): 1～7
36. 苏纪兰,袁业立. 2005. 中国近海水文 [M]. 北京: 海洋出版社
37. 苏育嵩. 黄东海地理环境概括、环流系统与中心渔船 [M]. 山东海洋学院学报,1986,16(1):12～27
38. 孙 松, 王 荣, 张光涛,杨 波,吉 鹏,张 芳. 黄海中华哲水蚤度夏机制初探 [J]. 海洋与湖沼, 2002 (浮游动物研究专辑): 92～99
39. 孙乃恩,孙东旭,朱德煦. 分子遗传学 [M]. 南京大学出版社,1990.pp.7～9
40. 唐启升,苏纪兰等. 2000. 中国海洋生态系统动力学研究 I:关键科学问题于研究发展战略 [M]. 科学出版社
41. 谭树华,林元烧,曹文清,陈钢,杨明. 黄、东海中华哲水蚤种群遗传的初步研究 I: 等位酶分析 [J]. 厦门大学学报(自然科学版),2003, 42(1): 87～91
42. 谭树华,曹文清,林元烧,李少菁,郭东晖. 黄、东海精致真刺水蚤种群遗传结构研究 [J]. 海洋科学,2004,28(4): 29～33
43. 徐兆礼,崔雪森,陈卫忠. 东海浮游桡足类的种类组成及优势种 [J]. 水产学报,2004,28(1): 35～40
44. 王桂忠,李少菁. 厦门港海区真刺唇角水蚤不同季节种群的同工酶分析 [J]. 海洋与湖沼. 1992, 23(4):657～662
45. 王 荣,张 经,黄大吉,孟田湘,金显仕. 2000. 东、黄海生态系统的基本特征. 引自:唐启升,苏纪兰等著. 中国海洋生态系统动力学研究 I: 关键科学问题与研究发展战略 [M]. 科学出版社,2000. pp. 34～42
46. 王 荣,陈亚瞿. 浮游动物种群的补充. 引自:唐启升,苏纪兰等著. 中国海洋生态系统动力学研究 I: 关键科学问题与研究发展战略 [M]. 科学出版社,2000. pp. 107～109
47. 王 荣,陈亚瞿,王 克,左 涛. 东海中华哲水蚤的年产量估算 [J]. 生态学报,2003, 23(6):1213～1215
48. 王新刚, 孙 松, 蒲新明, 张芳. 黄海冷水团区域中华哲水蚤的体长、体重、元素组成和代谢特征 [J]. 海洋与湖沼, 2002, (浮游动物研究专辑), pp. 45～50
49. 王雄进,康洁生,李 松. 厦门港中华哲水蚤体长、体重和碳、氮、氢含量的季节变化 [J]. 台湾海峡,1988,7(2): 173～179
50. 王雄进,康洁生,李 松. 厦门港中华哲水蚤的体长、体重、化学组成和呼吸率的研究 [J]. 甲壳动物学论文集,1990,2: 53～64
51. 王中仁. 1996. 植物等位酶分析 [M]. 科学出版社
52. 杨纪明. 渤海中华哲水蚤摄食的初步研究 [J]. 海洋与湖沼. 1997, 28(4): 376～382
53. ------. 渤海中华哲水蚤桡足幼体摄食的初步研究 [J]. 海洋水产研究, 1998,19(2): 1～4
54. 张 芳,孙 松. 中华哲水蚤生态学研究进展 [J]. 海洋科学, 2001, 25(11): 16～19
55. 张光涛,孙 松,张 芬,刘桂梅. 度夏过程中温度对中华哲水蚤生殖的伤害作用 [J]. 海洋与湖沼,2002,(浮游动物研究专辑): 85～90
56. 张秀梅,高天翔,王溪宏. 冲绳日本绒螯蟹线粒体 DNA 12S rRNA 序列的研究 [J]. 湛江海洋大学学报, 2000, 20(2): 11～15
57. 郑元甲,陈雪忠,程家骅,王云龙,沈新强,陈卫忠,李长松等. 东海大陆架生物资源与环境 [M]. 上海科学技术出版社,2003. pp.169～205
58. 郑重,郑执中. 十年来我国浮游动物的研究 [J]. 海洋与湖沼,1959,2(1): 214～222
59. 郑重. 厦门海洋浮游动物的初步研究 [J]. 厦门大学学报(自然科学版),1955,5: 1～16
60. 郑重,李少菁,连光山. 1992. 海洋桡足类生物学 [M]. 厦门:厦门大学出版社
61. 周美玉,林元烧,方旅平,郑连明,刘迟迟,曹文清. 甲醛溶液保存的中华哲水蚤基因组 DNA 提取和 PCR 扩增 [J]. 厦门大学学报(自然科学版),2005,44(3): 403～406
62. 祖元刚,孙 梅,康 乐. 生态适应与生态进化 [M]. 北京:高等教育出版社,施普林格出版社,2000
63. Battaglia B, Bisol PM and V Varotto. Genetic variability in marine and brackish populations of Tisbe holothuriae (Copepoda; Harpactacoida) [J]. Archaeological Zoology and Experimental Genetics. 1978.119:251-264
64. Bucklin A, Caudill CC and M Guarnieri. 1998. Population genetics and phylogeny of planktonic copepods. In: Molecular Approaches to the Study of the Ocean [M]. Edited by Keith E. Cooksey. Chapman and Hall (London). pp. 303-317
65. Bucklin A and TC Lajeunesse. Molecular genetic variation of Calanus pacificus (Copepod: Calanoida): preliminary evaluation of genetic structure and subspecific differentiation based on mtDNA sequence [C]. California Cooperative Oceanic Fisheries Investigation Reports, 1994, 25:45-51
66. Bucklin A and PH Wiebe. Low mitochondrial diversity and small effective population sizes of the copepods, Calanus finmarchicus and Nannocalanus minor: possible impact of climatic variation during recent glaciation [J]. J. Heredity, 1998, 89:383: 392
67. Bucklin A, Smolenack SB, Bentley AM and PH Wiebe. Gene flow patterns of the euphausiid, Meganyctiphanes norvegica, in the N Atlantic based on DNA sequences for mitochondrial cytochondrial cytochrome oxidase I and bytochrome b [J]. J. Plankton Res., 1997, 19: 1763-1791
68. Bucklin A. Methods for population genetic analysis of zooplankton [M]. ICES Zooplankton Methodology Manual. Acadimic Press. 2000
69. Bucklin A and Marcus NH. Genetic differentiation of populations of the planktonic copepod Labidocera aestiva [J]. Mar. Biol., 1985, 84: 219-224
70. Bucklin A, Reinecker MM and CNK Mooers. Genetic tracers of zooplankton transport in coastal filaments of the US west coast [J]. J. Geophys. Res., 1989, 94: 8277-8288
71. Bucklin A. Population genetic responses of the planktonic copepod Metredia pacifica to acoastal eddy in the California Current [J]. J. Geophys. Res., 1991, 96: 14799-14808
72. Bucklin A, Frost BW and TD Kocher. DNA sequence variation of the mitochondrial 16S rRNA in Calanus (Copepoda; Calanoida): intraspecific and interspecific patterns [J]. Molec. Mar. Biol. Biotech., 1992. 1: 397-407
73. Bucklin A, Frost BW and TD Kocher. Molecular systematics of seven species of Calanus and three species of Metridia species (Calanodia:Copepoda) [J]. Mar. Biol., 1995, 121:655-664
74. Bucklin A, Sundt R and G Dahle. The population genetics of Calanus finmarchicus in the North Atlantic. Proceedings of an ICES planning workshop for a TransAtlantic Study of Calanus finmarchicus [J]. Ophelia,1996, 44: 29-45.
75. Bucklin A and TD Kocher. Source regions for recruitment of Calanus finmarchicus to Georges Bank: evidence from molecular population genetic analysis of mtDNA [J]. Deep-Sea Research II. 1996, 43(7-8):1665-1681
76. Bucklin A, LaJeunesse TC, Curry E, Wallinga J and K Garrison. Molecular genetic diversity of the copepod, Nannocalanus minor: genetic evidence of species and population structure in the N. Atlantic Ocean [J]. J. Marine Research, 1996, 54: 285-310
77. Bucklin A, Bentley AM and SP Franzen. Distribution and relative abundance of the copepods, Pseudocalanus moultoni and P. newmani, on Georges Bank based on molecular identification of sibling species [J]. Mar. Biol, 1998, 132: 97-106
78. Bucklin A, Wiebe PH, Smolenack SB, Copley NJ and ME Clarke. Integrated biochemical, molecular genetic, and bioacoustical analysis of mesoscale variability of the euphausiid Nematoscelis difficilis in the California Current [J]. Deep-Sea Res., 2002, 49: 437-462
79. Bucklin A, Frost BW, Bradford-Grieve J, Allen LD and NJ Copley. Molecular systematic and phylogenetic assessment of 34 Calanoid copepod species of the Calanidae and Clausocalanidae [J]. Marine Biology, 2003, 142: 333-343.
80. Burton RS and SG Swisher. Population structure of the intertidal copepod Tigriopus californicus as revealed by field manipulation of allele frequencies [J]. Ocealogia., 1984. 65: 108-111
81. Burton RS. Evolutionary consequences of restricted gene flow among natural populations of the copepod, Tigriopus californicus [J]. Bull. Mar. Sci., 1986, 39: 526-535
82. Burton RS and BN Lee. Nuclear and mitochondrial gene genealogies and allozyme polymorphism across a major phylogeographic break in the copepod Tigriopus californicus [C]. Proc. Natl. Acad. Sci. USA, 1994, 91: 5197-5201
83. Caudill CC. 1995. Molecular evidence of population genetic differentiation and sibling species in Acartia tonsa (Copepoda: Calanoida) [T]. MA Thesis, Department of Zoology, University of New Hampshire, Durham, NH.
84. Clary DO and DR Wolstenholme. The mitochondrial DNA molecule of Drosophila yakuba: nucleotide sequence, gene organization and genetic code [J]. J. Mol. Evol., 1985, 22:252-271.
85. Chen QC. The marine zooplankton of Hong Kong [C]. In: Morton, BS and Tseng CK (eds), Proceedings of the First International Marine biological Workshop: The Marine Flora and Fauna of Hong Kong and Southern China. Hong Kong University Press, Hong Kong, pp.789-799
86. Chiba T. On the distribution of the plankton in the Eastern China Sea and Yellow Sea [J]. Bull. Japan Soc. Sci. Fisher., 1952, 17(7): 183-190
87. Corkett CJ and IA McLaren. 1978. The biology of Pseudocalanus [J]. Advances in Marine Biology, vol. 15. pp.1-233
88. Edmands S. Phylogeography of the intertidal copepod Tigriopus californicus reveals substantially reduced population differentiation at northern latitudes [J]. Molecular Ecol. 2001, 10: 1743-1750
89. Ekman S. 1953. Zoogeography of the sea [M]. London. pp. 321-324.
90. Enomoto Y. Studies on the food base in the Yellow and the East China Sea III. Notes on some dominant zooplankton [J]. Bull. Japan Soc. Sci. Fisher., 1963, 29(1): 7-13.
91. Folmer O, Black M, Hoen W, Lutz R and R Vrijenhoek. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates [J]. Molec. Mar. Biol. Biotech.,1994,3:294～299.
92. Humes AG. How many copepods? [J]. Hydrobiologia, 1994, 292/293: 1-7
93. Goswami U and Goswami SC. Karyological studies in the genus Acartia (copopoda) [J]. Current Science, 1973, 42(7): 242-243
94. ---------------------------------------. Karyology of the genus Labidocera Lubbock from the Laccadive Sea (Lakshadweep) [J]. Indian Journal of Marine Sciences, 1979, 8:259-261
95. ---------------------------------------. Chromosomal aberrations found in Paracalanus aculeatus (Gesbrecht) at the time of solar eclipse [J]. Mahasagar Bulletin of the National Institute of Oceanography, 1982, 15(1):59-62
96. --------------------------------------. A note on chromosomes of Pontellopsis herdmani and Pontella princes (Copepoda) from the Laccadive Sea [J]. Mahasagar Bulletin of the National Institute of Oceanography, 1984, 17(2):129-132
97. Guarnieri M. 1996. A preliminary study of genetic variation within and among populations of Diaptomus leptopus (Copepoda: Calanoida): a DNA sequence analysis of the mitochondrial cytochrome oxidase I gene [T]. MA Thesis, Department of Biology, Concordia University, Montreal, Quebec.
98. Han XF, Wang R and JP Wang. Digestive gut structure and activity of protease, amylase, and alkaline phosphatase in Calanus sinicus during summer in the Yellow Sea and the East China Sea [J]. J. Exp. Mar. Biol. Ecol., 2002, 270: 131-146
99. Hill SR, Allen LD and A Bucklin. Multiplexed species-specific PCR protocol to discriminate four N. Atlantic Calanus species, with an mtCOI gene tree for ten Calanus species [J]. Mar. Biol., 2001, 139: 279-287
100. Hirose E, Toda H, Saito Y and H Watanabe. Formation of the multiple-layered fertilization envelope ini the embryo of Calanus sinicus Brodsky (Copepoda: Calanoida) [J]. J. Crust. Biol. 1992, 12(2): 186-192
101. Hudson RR, Slatkin, M and WP Maddison. Estimation of levels of gene flow from DNA sequence data [J]. Genetics, 1992, 132: 583-589
102. Huang C, Uye S and T Onbe. Geographic distribution, seasonal life cycle, biomass and production of a planktonic copepod Calanus sinicus in the Inland Sea of Japan and its neighboring Pacific Ocean [J]. J. Plankton Res., 1993, 15: 1229-1246
103. Hulsemann K. Calanus sinicus Brodsky and C. jashnovi, nom. Nov (Copepoda: Calanoida) of the North-west Pacific Ocean: a comparison, with notes on the integumental pore pattern in Calanus s. str. [J]. Invertevr. Taxon, 1994, 8:1461-1482
104. Hwang JS, Chen QC and CK Wong. Taxonomic composition and grazing rate of calanoid copepods in coastal waters of northern Taiwan [J]. Crustaceana, 1998, 71: 378-389
105. Hwang JS, Chen QC and CK Wong. Taxonomic composition, density and biomass of free-living copepods in the coastal waters of southwestern Taiwan [J]. Crustaceana, 2003, 76: 193-206
106. Hwang JS and CK Wong. The China coastal current as a driving force for transporting Calanus sinicus (Copepoda: Calanoida) from its population centers to waters off Taiwan and Hong Kong during the winter northeast monsoom period [J]. J Plankton Res., 2005, 27(2): 205-210
107. Imabayashi H and T Endo. Distribution o near-bottom plankton in the Ohta River estuary, Hiroshima Bay, in relation to salinity [J]. Bulletin of the Plankton Society of Japan, 1986,33: 113-123
108. Jarman SN, Elliott NG, Nicol S and A McMinn. Molecular phylogenetics of circumglobal Euphausia species (Euphausiacea: Crustacea) [J]. Can. J. Fish. Aquat. Sci., 2000, 57 (Suppl. 3): 51-58
109. Kann LM and Wishner K. Genetic population structure of the copepod Calanus finmarchicus in the gulf of Maine: allozyme and amplified mitochondrial DNA variation [J]. Mar. Biol. 1996. 125: 65-75
110. Lan YC, Shih CT, Lee MA and HZ Shieh. Spring distribution of copepods in relation to water masses in the Northern Taiwan Strait [J]. Zoological Studies, 2004, 43(2): 332-343
111. Lazzaretto I and A Libertini. Karyological investigations of two populations of Tigriopus (Copepoda, Harpacticoida) from the Kerguelen region [J]. Curstacean Biology, 1985, 5(2):330-333
112. Lee CE. Global phylogeography of a cryptic copepod species complex and reproductive isolation between genetically proximate “populations” [J]. Evolution, 2000, 54(6): 2014-2027
113. Li C, Sun S, Wang R and X wang. Feeding and respiration rates of a planktonic copepod (Calanus sinicus) oversummering in Yellow Sea Cold Bottom Waters [J]. Mar. Biol., 2004, 145: 149-157
114. Lindeque PK, Harris RP, Jones MB and GR Smerdon. Simple molecular method to distinguish the identity of Calanus pecies (Copepoda:Calanoida) at any development stage[J]. Mar. Biol., 1999, 133:91-96.
115. Manning CA & A Bucklin. Multivariate ananysis of the copepod community of near-shore waters in the western Gulf of Maine [J]. Mar. Ecol. Progr. Ser., 2005, 292: 233-249
116. Marshall SM and Orr AP. 1955. The biology of a marine copepod, Calanus finmarchicus (Gunnerus) [M]. London: Oliver & Boyd.
117. Mauchline J, Blaxter JHS, Southward AJ and PA Tyler. 1998. The biology of Calanoid Copepods [M]. Advances in Marine Biology, vol. 33. pp.1-701.
118. Machida RJ, Miya MU, Nishida M and S Nishida. Complete mitochondrial DNA sequence of Tigriopus japonicus (Crustacea:Copepoda) [J]. Marine Biotechnolology, 2002, 4: 406-417.
119. Mori T. 1937. The pelagic copepods from the neighbouring waters of Japan [M]. Tokyo. pp. 150.
120. Nei M and S Kumar. 2000. Molecular evolution and phylogenetics [M]. Oxford University Press, Inc. [吕宝忠,钟 杨,高莉萍等译. 2002. 分子进化与系统发育 [M].高等教育出版社]
121. Roff DA and P Bentzen. The statistical analysis of mitochondrial DNA polymorphisms: X2 and the problem of small samples [J]. Mol. Biol. Evol., 1989, 6: 539-545
122. Page RDM and EC Holmes. 2000. Molecular evolution, a phylogenetic approach [M]. Blackwell Sc. Press, Inc.
123. Pu XM, Sun S, Yang B, Ji P, Zhang YS and F Zhang. The combined effects of temperature and food supply on Calanus sinicus in the southern Yellow Sea in summer [J]. J Plankton Res., 2004, 26(9): 1049-1057
124. Pu XM, Sun S, Yang B, Zhang GT and F Zhang. Life history strategies of Calanus sinicus in the southern Yellow Sea in summer [J]. J. Plankton Res., 2004, 26(9): 1059-1068
125. Schizas NV, Chandler GT and BC Coull. Differential survival of three mitochondrial lineages of a marine benthic copepod exposed to a pesticide mixture [J]. Environ. Sci. Tech.,2001, 35(3): 535-538
126. Sevigny JM and Odense P. Comparison of isoenzyme systems of Calanoid copepods by use of ultrathin agarose gel isoelectric focusing techniques [J]. Comp. Biochem. Physiol., 1985,80B: 455-461
127. Sevigny JM and IA McLaren. Protein polymorphism in six species of the genus Calanus [J]. Hydrobiologia, 1988, 167/168: 275-284
128. Sevigny JM, McLaren IA and BW Frost. Discrimination among and variation within species of Pseudocalanus Based on the GPI locus [J]. Mar. Biol., 1989, 102: 321-327
129. Sproston NG. Preliminary survey of the plankton of the Chu-San region, with a review of the relevant literature [J]. Sinensia, 1949, 20: 151-154.
130. Toda H and E Hirose. SEM and TEM observations on egg membranes of the two types of Calanus sinicus Eggs [J]. Proceedings of the fourth Intl. Conference on Copepoda; Bull. Plankton Soc. Japan, Spec.Vol. (1991): 613-617
131. Uye S. Temperature-dependent development and growth of Calanus sinicus (Copepoda: Calanoida) in the laboratory [J]. Hydrobiologia, 1988,167/168: 285-293
132. -------. Why does Calanus sinicus prosper in the shelf ecosystem of the Northwest Pacific Ocean? [J]. ICES Journal Marine Science, 2000, 57 (6): 1850-1855
133. Wang R, Zou T and K Wang. The Yellow Sea cold bottom water-an oversummering site for Calanus sinicus (Copepoda, Crustacea) [J]. J. Plankton res., 2003, 25, 169-183
134. Wong CK, Hwang JS and QC Chen. Taxonomic composition and grazing impact of calanoid copepods in coastal waters near nuclear power plants in northern Taiwan [J]. Zool. Stud., 1998, 37:330-339